LOCUS       DAFELO010000001       114040 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFELO010000001
VERSION     DAFELO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 114040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.001"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(68..778)
                     /locus_tag="LP452_000001"
     CDS             complement(68..778)
                     /locus_tag="LP452_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL2012769.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(775..2247)
                     /locus_tag="LP452_000002"
     CDS             complement(775..2247)
                     /locus_tag="LP452_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2012770.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2385..2858
                     /locus_tag="LP452_000003"
     CDS             2385..2858
                     /locus_tag="LP452_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2012771.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(3011..3562)
                     /locus_tag="LP452_000004"
     CDS             complement(3011..3562)
                     /locus_tag="LP452_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2012772.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3667..4197
                     /locus_tag="LP452_000005"
     CDS             3667..4197
                     /locus_tag="LP452_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL2012773.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4680..5123)
                     /locus_tag="LP452_000006"
     CDS             complement(4680..5123)
                     /locus_tag="LP452_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2012774.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5139..6299)
                     /locus_tag="LP452_000007"
     CDS             complement(5139..6299)
                     /locus_tag="LP452_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2012775.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6806..8506
                     /locus_tag="LP452_000008"
     CDS             6806..8506
                     /locus_tag="LP452_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012776.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8608..9441)
                     /locus_tag="LP452_000009"
     CDS             complement(8608..9441)
                     /locus_tag="LP452_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL2012777.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9745..11910)
                     /locus_tag="LP452_000010"
     CDS             complement(9745..11910)
                     /locus_tag="LP452_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2012778.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12170..14464
                     /locus_tag="LP452_000011"
     CDS             12170..14464
                     /locus_tag="LP452_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL2012779.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14457..15122
                     /gene="pgmB"
                     /locus_tag="LP452_000012"
     CDS             14457..15122
                     /gene="pgmB"
                     /locus_tag="LP452_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL2012780.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15115..16122
                     /locus_tag="LP452_000013"
     CDS             15115..16122
                     /locus_tag="LP452_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2012781.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16144..17163
                     /locus_tag="LP452_000014"
     CDS             16144..17163
                     /locus_tag="LP452_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2012782.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17444..18811)
                     /gene="nhaC"
                     /locus_tag="LP452_000015"
     CDS             complement(17444..18811)
                     /gene="nhaC"
                     /locus_tag="LP452_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL2012783.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18920..20341)
                     /locus_tag="LP452_000016"
     CDS             complement(18920..20341)
                     /locus_tag="LP452_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2012784.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20584..22461)
                     /gene="tdc"
                     /locus_tag="LP452_000017"
     CDS             complement(20584..22461)
                     /gene="tdc"
                     /locus_tag="LP452_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL2012785.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22752..24008)
                     /locus_tag="LP452_000018"
     CDS             complement(22752..24008)
                     /locus_tag="LP452_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL2012786.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24120..24325)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24588..25889
                     /locus_tag="LP452_000019"
     CDS             24588..25889
                     /locus_tag="LP452_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2012787.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25958..26557)
                     /locus_tag="LP452_000020"
     CDS             complement(25958..26557)
                     /locus_tag="LP452_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2012788.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26811..27692
                     /locus_tag="LP452_000021"
     CDS             26811..27692
                     /locus_tag="LP452_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2012789.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27705..29315
                     /locus_tag="LP452_000022"
     CDS             27705..29315
                     /locus_tag="LP452_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL2012790.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29392..30051)
                     /locus_tag="LP452_000023"
     CDS             complement(29392..30051)
                     /locus_tag="LP452_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2012791.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30334..30999)
                     /locus_tag="LP452_000024"
     CDS             complement(30334..30999)
                     /locus_tag="LP452_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL2012792.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31146..31751)
                     /gene="dhaL"
                     /locus_tag="LP452_000025"
     CDS             complement(31146..31751)
                     /gene="dhaL"
                     /locus_tag="LP452_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL2012793.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31779..32789)
                     /gene="dhaK"
                     /locus_tag="LP452_000026"
     CDS             complement(31779..32789)
                     /gene="dhaK"
                     /locus_tag="LP452_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL2012794.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32764..33210)
                     /gene="dhaM"
                     /locus_tag="LP452_000027"
     CDS             complement(32764..33210)
                     /gene="dhaM"
                     /locus_tag="LP452_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL2012795.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33313..33633
                     /locus_tag="LP452_000028"
     CDS             33313..33633
                     /locus_tag="LP452_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL2012796.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33745..34614)
                     /locus_tag="LP452_000029"
     CDS             complement(33745..34614)
                     /locus_tag="LP452_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2012797.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34710..36014)
                     /locus_tag="LP452_000030"
     CDS             complement(34710..36014)
                     /locus_tag="LP452_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2012798.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(36011..36520)
                     /locus_tag="LP452_000031"
     CDS             complement(36011..36520)
                     /locus_tag="LP452_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012799.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36695..38830)
                     /locus_tag="LP452_000032"
     CDS             complement(36695..38830)
                     /locus_tag="LP452_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2012800.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38913..40568)
                     /locus_tag="LP452_000033"
     CDS             complement(38913..40568)
                     /locus_tag="LP452_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2012801.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40570..40884)
                     /locus_tag="LP452_000034"
     CDS             complement(40570..40884)
                     /locus_tag="LP452_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2012802.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41054..41314
                     /locus_tag="LP452_000035"
     CDS             41054..41314
                     /locus_tag="LP452_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL2012803.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41328..41642
                     /locus_tag="LP452_000036"
     CDS             41328..41642
                     /locus_tag="LP452_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL2012804.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42372..42926)
                     /locus_tag="LP452_000037"
     CDS             complement(42372..42926)
                     /locus_tag="LP452_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL2012805.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43052..44981)
                     /locus_tag="LP452_000038"
                     /pseudo
     CDS             complement(43052..44981)
                     /locus_tag="LP452_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45287..46003)
                     /locus_tag="LP452_000039"
     CDS             complement(45287..46003)
                     /locus_tag="LP452_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL2012806.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(46004..47827)
                     /gene="glpO"
                     /locus_tag="LP452_000040"
     CDS             complement(46004..47827)
                     /gene="glpO"
                     /locus_tag="LP452_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL2012807.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47841..49337)
                     /gene="glpK"
                     /locus_tag="LP452_000041"
     CDS             complement(47841..49337)
                     /gene="glpK"
                     /locus_tag="LP452_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL2012808.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49473..50933)
                     /locus_tag="LP452_000042"
     CDS             complement(49473..50933)
                     /locus_tag="LP452_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2012809.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50923..52218)
                     /locus_tag="LP452_000043"
     CDS             complement(50923..52218)
                     /locus_tag="LP452_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2012810.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52550..53227)
                     /locus_tag="LP452_000044"
     CDS             complement(52550..53227)
                     /locus_tag="LP452_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL2012811.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53257..54348)
                     /locus_tag="LP452_000045"
     CDS             complement(53257..54348)
                     /locus_tag="LP452_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL2012812.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54449..54883)
                     /locus_tag="LP452_000046"
     CDS             complement(54449..54883)
                     /locus_tag="LP452_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2012813.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55467..56924)
                     /gene="ascB"
                     /locus_tag="LP452_000047"
     CDS             complement(55467..56924)
                     /gene="ascB"
                     /locus_tag="LP452_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2012814.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56921..58816)
                     /locus_tag="LP452_000048"
     CDS             complement(56921..58816)
                     /locus_tag="LP452_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2012815.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58874..60649)
                     /locus_tag="LP452_000049"
     CDS             complement(58874..60649)
                     /locus_tag="LP452_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2012816.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60699..62195)
                     /locus_tag="LP452_000050"
     CDS             complement(60699..62195)
                     /locus_tag="LP452_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2012817.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62389..64011)
                     /locus_tag="LP452_000051"
     CDS             complement(62389..64011)
                     /locus_tag="LP452_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL2012818.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64339..65652)
                     /locus_tag="LP452_000052"
     CDS             complement(64339..65652)
                     /locus_tag="LP452_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL2012819.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65897..66121)
                     /locus_tag="LP452_000053"
     CDS             complement(65897..66121)
                     /locus_tag="LP452_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012820.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66141..66563)
                     /locus_tag="LP452_000054"
     CDS             complement(66141..66563)
                     /locus_tag="LP452_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL2012821.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66706..67416)
                     /locus_tag="LP452_000055"
     CDS             complement(66706..67416)
                     /locus_tag="LP452_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012822.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67434..67931)
                     /locus_tag="LP452_000056"
     CDS             complement(67434..67931)
                     /locus_tag="LP452_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2012823.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67968..68420)
                     /locus_tag="LP452_000057"
     CDS             complement(67968..68420)
                     /locus_tag="LP452_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2012824.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68555..69160)
                     /locus_tag="LP452_000058"
     CDS             complement(68555..69160)
                     /locus_tag="LP452_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2012825.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69610..70230)
                     /locus_tag="LP452_000059"
     CDS             complement(69610..70230)
                     /locus_tag="LP452_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2012826.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70232..71101)
                     /locus_tag="LP452_000060"
     CDS             complement(70232..71101)
                     /locus_tag="LP452_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL2012827.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71124..71531)
                     /locus_tag="LP452_000061"
     CDS             complement(71124..71531)
                     /locus_tag="LP452_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL2012828.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71550..72839)
                     /locus_tag="LP452_000062"
     CDS             complement(71550..72839)
                     /locus_tag="LP452_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2012829.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72866..73168)
                     /locus_tag="LP452_000063"
     CDS             complement(72866..73168)
                     /locus_tag="LP452_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2012830.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73193..75286)
                     /locus_tag="LP452_000064"
     CDS             complement(73193..75286)
                     /locus_tag="LP452_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2012831.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75775..76539)
                     /locus_tag="LP452_000065"
     CDS             complement(75775..76539)
                     /locus_tag="LP452_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL2012832.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76650..77519)
                     /gene="mdcB"
                     /locus_tag="LP452_000066"
     CDS             complement(76650..77519)
                     /gene="mdcB"
                     /locus_tag="LP452_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL2012833.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77544..78620)
                     /locus_tag="LP452_000067"
     CDS             complement(77544..78620)
                     /locus_tag="LP452_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL2012834.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78644..79237)
                     /locus_tag="LP452_000068"
     CDS             complement(78644..79237)
                     /locus_tag="LP452_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL2012835.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79241..80917)
                     /gene="mdcD"
                     /locus_tag="LP452_000069"
     CDS             complement(79241..80917)
                     /gene="mdcD"
                     /locus_tag="LP452_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL2012836.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80907..81206)
                     /locus_tag="LP452_000070"
     CDS             complement(80907..81206)
                     /locus_tag="LP452_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL2012837.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81308..82954)
                     /gene="mdcA"
                     /locus_tag="LP452_000071"
     CDS             complement(81308..82954)
                     /gene="mdcA"
                     /locus_tag="LP452_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL2012838.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(82961..83872)
                     /locus_tag="LP452_000072"
     CDS             complement(82961..83872)
                     /locus_tag="LP452_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL2012839.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            83992..84849
                     /locus_tag="LP452_000073"
     CDS             83992..84849
                     /locus_tag="LP452_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2012840.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(85036..85398)
                     /locus_tag="LP452_000074"
     CDS             complement(85036..85398)
                     /locus_tag="LP452_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL2012841.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            85744..87183
                     /locus_tag="LP452_000075"
     CDS             85744..87183
                     /locus_tag="LP452_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2012842.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87190..89736)
                     /locus_tag="LP452_000076"
     CDS             complement(87190..89736)
                     /locus_tag="LP452_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL2012843.1"
                     /translation="MVQIDGRRCIVNYQTSTEGLSQEERQKRLTDQGYNEIKARQQTK
                     RWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEESL
                     NGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVEES
                     ILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKINQ
                     ALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIALVV
                     SMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNEMT
                     VVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKIEA
                     KIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARKGQ
                     RVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKMIT
                     GDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLRIV
                     KALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTIAK
                     AVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTITL
                     SYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQ
                     QTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQ
                     QLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89855..91186)
                     /locus_tag="LP452_000077"
     CDS             complement(89855..91186)
                     /locus_tag="LP452_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2012844.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91152..92630)
                     /locus_tag="LP452_000078"
     CDS             complement(91152..92630)
                     /locus_tag="LP452_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2012845.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(92935..94770)
                     /gene="tdc"
                     /locus_tag="LP452_000079"
     CDS             complement(92935..94770)
                     /gene="tdc"
                     /locus_tag="LP452_000079"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL2012846.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            95240..96667
                     /locus_tag="LP452_000080"
     CDS             95240..96667
                     /locus_tag="LP452_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2012847.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(96759..97124)
                     /locus_tag="LP452_000081"
     CDS             complement(96759..97124)
                     /locus_tag="LP452_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL2012848.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(97121..97486)
                     /locus_tag="LP452_000082"
     CDS             complement(97121..97486)
                     /locus_tag="LP452_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL2012849.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(97678..97926)
                     /locus_tag="LP452_000083"
     CDS             complement(97678..97926)
                     /locus_tag="LP452_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012850.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            97925..98053
                     /locus_tag="LP452_000084"
     CDS             97925..98053
                     /locus_tag="LP452_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL2012851.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            98110..99804
                     /locus_tag="LP452_000085"
     CDS             98110..99804
                     /locus_tag="LP452_000085"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL2012852.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            99808..99927
                     /locus_tag="LP452_000086"
                     /pseudo
     CDS             99808..99927
                     /locus_tag="LP452_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            99904..100200
                     /locus_tag="LP452_000087"
     CDS             99904..100200
                     /locus_tag="LP452_000087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012853.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            100578..101711
                     /locus_tag="LP452_000088"
     CDS             100578..101711
                     /locus_tag="LP452_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL2012854.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            101742..102062
                     /locus_tag="LP452_000089"
     CDS             101742..102062
                     /locus_tag="LP452_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL2012855.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(102091..102555)
                     /locus_tag="LP452_000090"
     CDS             complement(102091..102555)
                     /locus_tag="LP452_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2012856.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(102687..103941)
                     /locus_tag="LP452_000091"
                     /pseudo
     CDS             complement(102687..103941)
                     /locus_tag="LP452_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(103970..104287)
                     /locus_tag="LP452_000092"
     CDS             complement(103970..104287)
                     /locus_tag="LP452_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2012857.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(104313..104633)
                     /locus_tag="LP452_000093"
     CDS             complement(104313..104633)
                     /locus_tag="LP452_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2012858.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(105097..106512)
                     /locus_tag="LP452_000094"
     CDS             complement(105097..106512)
                     /locus_tag="LP452_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2012859.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(106529..107845)
                     /locus_tag="LP452_000095"
     CDS             complement(106529..107845)
                     /locus_tag="LP452_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2012860.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(107995..108693)
                     /locus_tag="LP452_000096"
     CDS             complement(107995..108693)
                     /locus_tag="LP452_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL2012861.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(108982..109938)
                     /locus_tag="LP452_000097"
     CDS             complement(108982..109938)
                     /locus_tag="LP452_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2012862.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(110051..110884)
                     /gene="msrA"
                     /locus_tag="LP452_000098"
     CDS             complement(110051..110884)
                     /gene="msrA"
                     /locus_tag="LP452_000098"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2012863.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(111059..112687)
                     /locus_tag="LP452_000099"
     CDS             complement(111059..112687)
                     /locus_tag="LP452_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012864.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(112674..114008)
                     /locus_tag="LP452_000100"
     CDS             complement(112674..114008)
                     /locus_tag="LP452_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012865.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        33564 a        23811 c        19633 g        37032 t
ORIGIN      
        1 atctgattgt tcttactgct tgatgcagag cagctttttc acaacctcct acatggtgtt
       61 caaaatatta ttcgcctttt gcttctttca ctaattttgc gaatgcttgt tgttggaatt
      121 cagcagctaa ggctttcgta cgttgttctt gctctgcttt gctcattttt ggattttcag
      181 tcattgcttt taccataccg tcttgaacat aagcttttgc ttcactttcc atcgtacttt
      241 gctgtttctt cactaattca gctaacttgg ctgcttggtc cgtacttaat tctacccatg
      301 tctctgggat tttgaatgta ttcactcggc tgtcatattc gtgattgtta tcagcaatat
      361 tgaaccagaa tttatacatg tcgtttttgt attcccaata agtagttttc gtcacttttt
      421 cagcatcgcc gtctgtcttt tctactttat ttgtttcgtt atccgtacca gttgcttttg
      481 gtttttcttg tttttcattt gttcggtaaa gatacacttt ttcttttcca ttccctaaag
      541 ctttatacaa aagcatatct gctccttggg aatcagcact ggaaacaatt ttttctgttt
      601 ttgtttccgt tactttttcc attccaaagt gattggacaa attagcgacg atcaaactta
      661 gtgaagcgac gaagattagt ccgaaaatca aagataaaaa ggtttgccat gtttttttag
      721 cgaacacgtt cgttaaagca aaagcaagca agctgacaat caaaactcca ataatcatgc
      781 attatccccc gctttcatta gaacaggttc ttttttcttc aataaaaagg caataataaa
      841 gccaagaatc ccaaagatga tcgctacgat aaacgcggca tggtaacctg acagtgtggc
      901 attgattgcc tgatctttat aagctaatgg cgtattatcc aatacagatt tgcttgggat
      961 atttgctttt gtcacattcg tcaacacact gatcaaaata gctgtaccaa tcgagcttgc
     1021 gatttgacgg aatgtattat ttaccgctgt cccatgactg atcaagttat ttggcaatgc
     1081 attcatacca gaagtcgtta ccggcatcat gaccatggag atcccaaaca tacggatcgc
     1141 ataaaggatc acgatatata aaactggagt cgtttttgtc aagaaagcaa atggtgcagt
     1201 tgccgctgtc aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt atttatcgaa
     1261 aatcgcaccc gtcacaggca tcatagcccc catgatcaat gcacccggca acaacatcaa
     1321 gccagaatgg aatgctgatt ctccacgaat attttgaata tacaatggta cgaccatttc
     1381 tgcccctacc atcgccatat ttgttacacc agctaaaaca gctgcaatcg tgaatgtaga
     1441 tgatttgaat acacgaagtt ccaagaatgg atgttccaaa tgtaattgac gccatgagaa
     1501 caaggcgatg aaaatcacac caacaactaa gaaaccaatt actttcaagc ttccccagcc
     1561 gtcatctcct acactagaaa atccgtaaag taaactaccg aatccaatcg ttgataaaat
     1621 agcggataaa atatcaatgc taggatttga caatggaata acacttttca ttaggaagaa
     1681 tgctaaaacc aagacaagaa caacgattgg cagtaccata ccaaacaagt cacgccaatg
     1741 gtagttatca atgatataac cagataatgt agggcctaaa gcaggagcaa gtccaataac
     1801 gatccctgac atccccattg ctgatcctct tttttctggc gggaaaatag atagcatgat
     1861 attttgcaat aatggcatcg atactcccac accgcaagct tggatcaaac gaccgattag
     1921 taatgtacta aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa agatcgtcat
     1981 ggcagaaaga tataaatttc ttgaactgaa tttattgatc agccatgctg ttattgggat
     2041 catgatcccg ttgactagca agaaaccagt cgttaaccat tgcacatcag acgctgaaat
     2101 atcgaaagct ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat tcaaaacggt
     2161 acaaaatgta ccaattaata agacaacaac taaaagtgtc cgattatatg gttttccgta
     2221 aatatctacg ggttggttgt tactcatatt tctccttctt tctgaatttt ttctgaaact
     2281 actagaatac tttagtccta aattcgctat ttgtcaacct acttgacaca taagttaata
     2341 cggatataat taagggaagc aagaggacaa aaggagtgac aaaaatggtt ggggcttccc
     2401 ttaaacgttt gttggaaaat gatcaattgg ttgttggcat cagtgaatta agtgatatcg
     2461 taggtgtgtc tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt cgttcgatcg
     2521 aagctgatgc gaattgtcct agaaaatatc gtttacctac agtcataaaa gtagaactga
     2581 tcaaaaaata tttagatgac ggatttactt tgtcaaaagc tgcagaaaag gcagaaatcc
     2641 gccaaaagaa aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa gatatcgaat
     2701 tgatcgacga acgctatacg attttacaaa tcggtaactt caaagaagag aatgctaaga
     2761 tgtacatcat tcatgacaac gagacagacg aattaactta caagctcctt cctgtcgatg
     2821 aaaaagctga ctacgaacag ttgttaaaag aactctaaac gccactaatc tcctaagtac
     2881 caagcttttt aataagtaga tgctacataa atacttgaaa aaaggcctgt ttgaacataa
     2941 gaatatccga acggtgatct gcgtttcttt catatagaaa ggaattctcc atcactgttc
     3001 ggatattttt ttattttttt cggttacttt cttctaatac atctgttagc caatttgcat
     3061 attctgtttc ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa ttttcatgtt
     3121 tttctttttc agtaggaaag atcgtttttt ttcgctctaa tagatgaagt cgtttttctt
     3181 catgaaggtc acgctgctgc tgaataattt ctactaatgc cggatcattc acgtctttga
     3241 cgaaatacaa tttcagaaca aattcgtctc gattaaccgg tagttcattg gtgggtgtat
     3301 gaatccactc tgccaattgt tccttccctt cttctgttaa ttcataaact ttcttttcca
     3361 atttttcacc tgtgatctct acttgatgct gaatgattcc ttgctcttcc atttttgcaa
     3421 gttcaggata aatctgacta tgttttgctt gccaaaattc tcctatttca tttttgaacg
     3481 attgattcaa ttcatatcct gtcatcttct tcttacttaa taaccctaac aaaatatatc
     3541 gaagtgtatt ttttcttgcc atcagttttc cctccttgac aaatctttaa atccattcta
     3601 tcataaatga gtaatcgaaa acatgtagtt tattacatgt tttaatttat aaggagatgc
     3661 ttaaacatga aagaatttaa agaattggaa gattttattg gaactcactt tatctatacg
     3721 tatgacaatg gttgggaata tgaatggtat gccaaaaatg accatactgt tgattaccgt
     3781 atccatggcg gaatggtcaa aggccgttgg gtaaaagacc aagaagctga tatcgttaaa
     3841 ttgacggacg gcgtattcaa aatcacttgg acagagccaa cagggacaga cgtggcattg
     3901 gactttatgc caaatgaaaa gaaacttcac ggaatgatct ttttccctaa atgggttgaa
     3961 gaacatccag aaatcactgt tactttccaa aacgaacata tcgcagaaat ggaagcagct
     4021 cgtgaaaaat atgaaactta tccaaaatta cttgttcctg aatttgctac gatcacttat
     4081 atgggtgatg caggtataga caatgaagat gttattagcg aagaaccata cgatggaatg
     4141 ccagatgata tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt aaaataaggt
     4201 tgtgaaagag aggttgtgaa atcagcgttc tgacctgagt attagagaaa gaaacggaaa
     4261 aatagttcct catgttttgc cgttttttga gctaataccg agggtcagct gattgaacac
     4321 cgtttatcaa gaggttgtga aatcagcgtt atgctttgag taataagaac gctagttaga
     4381 aaatagcttt tcatattttc taggctagtg ggcttattac cgagaagcag ccgtttgaac
     4441 accgtttatc aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaatgg
     4501 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     4561 caccgtttat caaagattgt aacaactgca tattcacaca gttaaattag tgacttcaaa
     4621 atgaaataat gctcccagca aaaaattagg ctgggagcat tatttcgtta ttctgttcat
     4681 caaaaatcag cgttttgatt ccatttcatc acaagttcct cctcatttac tgcttcatct
     4741 ttttgctgtg tttgaattgt aaaaccttgt gaaagataga aatgaatagc tgattgattt
     4801 ttttgataga ctgtcaaagt cagtgtttta ttggtttctt ttattttttt cattagttgc
     4861 gtaccgattc tttgttgccg gtattccctt ttcacaaaga ttcctgcgat aaactgatca
     4921 atcaccccag caaaaccaat aatttcttga tctatttctg ctaagtaaat cactgcatct
     4981 ggtaaagctt tcctaacaga ttctacttga ttctcccaat aagttgatgg aatgaattca
     5041 tgagaatcca agttagcatg cagccaaatc gatagaacag attccagttc tttggcagac
     5101 aattttgttg ttgtgcggat cattttttct ctccttactt attcattgat tttaaaataa
     5161 aaaccatttt tttgaagctc ttttacaagt tttcgcccct caaaaaagta tatcttctct
     5221 tgttttactt ttctaatacc aaaagaaaca gcaactttag gatcatcagc tgaaacgatt
     5281 cgataagttc caccaggcaa ttgatccagc ttgactttcc catgatggtc gctttgtact
     5341 gaaaacggca caccatttct agtaagtgtt tttttcttat gtttcaaaat gaatgaacta
     5401 ccatttaccg gtttccctga cgaatcacta ataataaatg aaaaatcaag cactttttta
     5461 cttaatctat actgatagat tagtcgtccg ataaacacta acagtatgac tacagctatt
     5521 atcgttcctg caagaataaa ccattgccgc atggttcttt cctgatttcc ttttccttct
     5581 tcttttttta ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg
     5641 ttgatcatat acggcgtaca tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt
     5701 agaacagacg tgtcatctgg cagtaccact tgtatatttt taacttgata agccaatttc
     5761 tcccccaaaa cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat
     5821 aaccgtcgat taggtaaacc gctgtgagca gagatcactg aatgcgtcga atttccacca
     5881 agcgggaaag aggttccttg caagacgcct gctccatttt caaggatcct accattgagc
     5941 cgatcataaa gtgaaatatt taattggatc ttaggtatat tgatacttcc gatcagccat
     6001 tcctctaatt ggctggatgt tgtactttca tttctgttct tttcattgaa aggatcagta
     6061 ccaggattga atccttggtc agttaatcgt tggtttttct cctccatttt ctttcgctgc
     6121 acttcaattt cagctgcggt ccgtttttgt acttgttcga tacgtttttg atcaataaaa
     6181 ctatttaaag aatccacata aaatggatat aaagcgatca gtaaccctac gacgatacat
     6241 attaataaaa acaaacgaaa caaaagtcga cgagactgtt tttgcttact ctgcttcaca
     6301 tgtgtcctcc cttgcaagtt tttttgttct ttcttttatt atatgggttc aggaaaaaaa
     6361 tagaaaatga aagccgatga ggttgtgaaa aagctgtcct gcatcaagta ataagtacag
     6421 cccaaaaaaa tagctactca tattttttgt tgggttgggc ttattaccgc agatgcaagc
     6481 ttttgaacac cgtttaagga agaggttgtg aaaactgcgt tatgctttga gtaataagag
     6541 cgctagtcag aaaatagctt ctcatatttt ctaggctagt gagcttatta ccgagaagca
     6601 gctgattgaa caccgtttac cgagaggtca tgaaaaagct actttgtttc gagtattaag
     6661 aaaaattctg tttcaaaaca aggagatgca acaaaactta tgtcgcatcc tctctcctta
     6721 gaactttcac ttatgttttt cttgtaaaat tcataaatat tttcatgaat tttctaattt
     6781 cttgcctacc tctcgcatag gatatatgat gaatttatct aaagtttgca aaggagttcg
     6841 acaattgaaa aaatatggcc catttttagc aatttttttc attgctttgg catttctcat
     6901 gcctcattta ttcacacata aactaatttt gggtgctgat gccccattcc attacaatcg
     6961 gttctatgat gccgcgcaac aaattaaata tggggatttc cattattttc cttcccttta
     7021 tggattccag caaagtggca ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt
     7081 acaaggattg atcgtgttgc ttgcaggtag ctggtttcat tatcagattc tttcaaattt
     7141 cattctttat cttatttcag gattttctat gctgatttta ctgagatatg tcaatgttcg
     7201 aaattgggta agtgtaccag tagccgtttt attcatgagt acgtattcta ttcaatactg
     7261 gtctatcaac caaggattca ctagctgggg gacttgtttt tatcctttat gtatgatccc
     7321 tattgctgat ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg
     7381 acttatgaca cagatccatt tactgtcttc gatcatgtta gctttgatgt atatcccttt
     7441 ttatctttat tactttttta accagactga aaaaggaaaa gcactgcttc aactgattaa
     7501 gtcggtcttg ctttattttg ttctgacagc aaatatttgg gcaggattag ctattgttta
     7561 cagtgggaat aagattcttc ctccctttgt taacagagaa atgagtcaaa aggcgataga
     7621 tctggatggc agttattggc tccattaccc taaggccttt cctatattga tattatcagg
     7681 ttttttgctt tttattttct ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta
     7741 cacaagtctt gttttctttt ttctttctac tagcttgatt ccatggacga cattgatcga
     7801 aaaaaatgtg ccttttatta gtttgatcca gtttccattt cgatttttcg tgccatttac
     7861 gctattgctt ctactttacc ttgccctttc actgaaccag tggtctgaac aaaagtggtt
     7921 ccgtctttta agtatatttg gcattcttat tttttcagca cagacgatcc agaacttata
     7981 ccagcatctg gaaaagtggg aaaatgaaac atttgtcagt cgacatactt atctattcga
     8041 tacgccggaa gaagcaagga aaacattctt cagcaaagat atgtctacca gcttgttcgt
     8101 tttccaaaaa accacacctg actatctgcc aatctataag gagacaaaag acaataaata
     8161 cgaccggtat aacgaagaaa tactagaaaa cgaaacaact tttataaaaa cacatccagg
     8221 cggtcttttg acaatcaagt ggaaaaacac tggggacaaa actgttcata tccctgttat
     8281 cgtctacgat cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac
     8341 agatatcggg accccaatcg tgaagcagca aaaaggaatc aacgagctca cgcttcatta
     8401 tcaaacgcca atctatttct atttcattct ttctcttact ttgattggct ggtttacctt
     8461 gctatgttta tttatttatc atagatacaa gcttttacgt gcttaggcaa aagggataaa
     8521 tacatccgat caaaaaactc tccaaacggt ataatcgttt ggagagtttt attttaatca
     8581 gtttttttat gttatatgtt atatttttta ttcgttcaca tctaattcga tttccacccc
     8641 gtagtgatca ctgatgattg gactatcgat cccatcaaaa atccgttgat attttttaac
     8701 ctgcattcct tcagggacga aaataaaatc tatacgcaat ttttctgtat tttgttccca
     8761 accatctatt ttcttttcca cagtagctgc ttttcctttt tcattcgcga caacatacga
     8821 atcttgaaga agaaggcaac tttctcttac agtataataa ccttgagagt ctactggatt
     8881 attgaaatca cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt
     8941 tttccactca aaataaaagc cacttttttt atctgtccac caagaaaagt ggcagctcga
     9001 gacaaaaatg tcctgatcat ctatctttgt tttcccaatc aagatcttgc gtgtctgata
     9061 atcttctttc ttttgaaatt gggagacggt aaggacctga ctttccaatg tttttttcgt
     9121 aagcagtgca tttccttctt catatttatc ataaccgatg tggctcattt cccaactcca
     9181 aaaatactct tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa aattatcttt
     9241 acggataggt acttgattct ttaccggaca gaacttcatt agcttcgcag gttctacttc
     9301 ttcagattcc agtaattggt tgatttcttg taaagcaatc acttcataat cttctaacag
     9361 gatcttatct gctaattctt gcaatttttt ctctggttct ggctcaagcc agctatgcgt
     9421 gttgagtgtc atgaatttca ttggttttct ccttgtcttc atttcgaaac aaaagggttg
     9481 tgacaaaatt tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa ctcctcagta
     9541 ataagctcaa tggaccagga gctatttttt gtttggctgt ttttatcact tggtgcaggg
     9601 cagctttttc acgacctctc cactaaacag tattcaaact gctgcttctc ggtaataagc
     9661 ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatg acttgatgca
     9721 ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt taccagctgt
     9781 cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa atacgatcat
     9841 gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag ctaatttcgt
     9901 tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct gattcatttg
     9961 gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt ccagcccaat
    10021 tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa caacttctct
    10081 tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata ccggatcttt
    10141 tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt gttctatacc
    10201 taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg gctctggaat
    10261 ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg cttttggtcc
    10321 ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg ctcgtttcca
    10381 ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc gagtcataca
    10441 agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct ggaccacttg
    10501 ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt cattgtcgta
    10561 atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat ttgctaagaa
    10621 atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat ctccacctag
    10681 acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa atgaatggac
    10741 ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg aataaatgac
    10801 atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta cacctgttag
    10861 aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct tatcaggatc
    10921 gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac caatcatttg
    10981 tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt gcgacatatc
    11041 ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg gatcttgccc
    11101 aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc caagctgtgt
    11161 atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta aacgttctaa
    11221 tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg caatccataa
    11281 accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa tcaatgccac
    11341 gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga aaaatgacaa
    11401 agcatccggc aattttctat aattatagta cttattataa gccattgccc cgacaaatcc
    11461 agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata cacttgtaaa
    11521 aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc
    11581 aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta aaataaatga
    11641 gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc ctattgctaa
    11701 agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa ttactccgcc
    11761 cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc ctatactgat
    11821 catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc caaatttttg
    11881 ccaaaactca aaactaaata tctttttcat tccgtttagc tcctttacgc aatcggtttc
    11941 gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt tatttttctg
    12001 tctataccat tctataaaac ctataatacg tgtgttccct tctataattt tctcctaaaa
    12061 atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga cttgtgcaat
    12121 cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa tgaaacagat
    12181 caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag ataaaaaaaa
    12241 tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga tgcgcgggaa
    12301 ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg gtgtctggta
    12361 tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact ttggaaaagt
    12421 gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc tgatcgactt
    12481 ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg gtgtattatc
    12541 tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg aacgttttct
    12601 gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc ttgaaggtag
    12661 cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag acagcaacta
    12721 tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg gctatttgac
    12781 gactaaaact attccaaaca actttgggat tgaacaattt actgttactg cagcaatgcg
    12841 ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt tagcattaca
    12901 tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa aagaaattat
    12961 cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg tctcagaatt
    13021 aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg cttgggcaga
    13081 acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac aaggtattcg
    13141 attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat tgaatatcgg
    13201 ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata cagaagcgta
    13261 tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc tgctgaaata
    13321 tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat taaaaggcgc
    13381 gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg aaatcacatt
    13441 cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata cgaattacac
    13501 cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt
    13561 ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc acggtgtgac
    13621 cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata cgatcgcagc
    13681 ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag ctgctatcac
    13741 tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt acttcccagt
    13801 tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag atctcatgcc
    13861 tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt gggacaaaat
    13921 ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt tcttcaatga
    13981 tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca
    14041 tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag gaatggaaag
    14101 caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt acaataacga
    14161 cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga ttgtccaagg
    14221 atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc tgccaaaaac
    14281 ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca tctcagtcac
    14341 agatgaacaa gttactttca agttattaga aggttctcct ctagaaattt acgtctatga
    14401 acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg aagcaaatgt
    14461 ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa ttccattatc
    14521 gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca ttcaacgaac
    14581 agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat ggcggaaaag
    14641 aacactcttt tacaaaggaa gaatttgcag aattagcgaa aagaaaaaac gattattatt
    14701 tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg cctttactgg
    14761 attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa aacgggccat
    14821 tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac ccggcagaag
    14881 caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct gtcggactaa
    14941 aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc atcctctcaa
    15001 gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag atcccgcttg
    15061 tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtggcat gaccatggat
    15121 aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat cacattgaag
    15181 aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca aatcttcgca
    15241 ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt
    15301 ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag gatccgggat
    15361 gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat ccacggtggc
    15421 acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc atcatcaata
    15481 ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat cactgctacg
    15541 atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag tagccttgtt
    15601 gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa tggaacaaga
    15661 gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt agatcaagaa
    15721 aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc
    15781 ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt atttcttttg
    15841 catcatccta ggctttccaa aacttcctta caattattcg agaagcattc tggtcgccaa
    15901 atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg attcgaagct
    15961 gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat cgaaccgcaa
    16021 gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca tcctggtcaa
    16081 gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa aggtgttttt
    16141 atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc
    16201 tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa agtccgaaaa
    16261 attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg aaaacgcatt
    16321 tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat cggtaaccca
    16381 ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga tatgactaca
    16441 gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg tatgcattta
    16501 caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatac tgtcattgat
    16561 tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca tcatgagaac
    16621 gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac agaatttttg
    16681 atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga aagtttgtat
    16741 ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc
    16801 agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac
    16861 gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat gcagttggcg
    16921 caaatgtatg gctatgtcat tccagagaca ctctccgtca ttagcttcaa caactcgatc
    16981 ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc cgaattagga
    17041 cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag tggcgtacgt
    17101 ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga aaacccatac
    17161 taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt tttatgtaaa
    17221 agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga caaaagtaaa
    17281 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
    17341 ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt cttcttattt
    17401 cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt tttacacgtc
    17461 gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc gagaaaaagg
    17521 cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat gtgccgctga
    17581 ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt cgtgtcaaat
    17641 aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct ggaagaatga
    17701 tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag gcagtaaacc
    17761 cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg gtatcaacga
    17821 tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa ctagcagaac
    17881 ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga tcaggtgtat
    17941 ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa cttttctcat
    18001 ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg atagccggaa
    18061 tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa ggagagatcc
    18121 aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg gctgaatgac
    18181 tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg aaatccgtat
    18241 aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct gccaagttgg
    18301 tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg actgcgcctg
    18361 tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca attccaatcg
    18421 tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa acaagaaatg
    18481 ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata atcgtaggga
    18541 tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa atcaggattg
    18601 gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc catgtgaact
    18661 ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg atatgtggcg
    18721 agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc aatacagcga
    18781 tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa atgaaaaggc
    18841 cctgccatga aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc
    18901 ggctgaaaga cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag
    18961 aatctttctt gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg
    19021 ctactaataa tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga
    19081 tagagaagat cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc
    19141 ggtaaggacg ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca
    19201 aaatgtacac gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac
    19261 ttacgtcatc cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta
    19321 gaacgacatt tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa
    19381 ggaatccttc acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata
    19441 attgtaaaag cacaccaata aataccatga agctgaagat atttccgatc acttctggta
    19501 agcctaggat ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc
    19561 catctggtac gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga
    19621 tcaaagcgat aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt
    19681 aaacagaact catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg
    19741 aatcaatatg ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt
    19801 ttgagactag tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg
    19861 ggatatatac ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca
    19921 agttcaagat cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat
    19981 tattgccaag ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga
    20041 ccattaccat acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt
    20101 tgctgcccag tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg
    20161 aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat
    20221 aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca
    20281 gtgccattgt cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca
    20341 ccgttgacac tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc
    20401 aataaacgaa acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg
    20461 tttcttcaaa gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt
    20521 cagatccaaa catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct
    20581 tgcttatttt gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg
    20641 aagagctgct ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg
    20701 tgtcattacg ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga
    20761 gaatcctagt tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc
    20821 agtatgtgaa gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc
    20881 atatacgtca tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa
    20941 tacatagtct accatattga aatctggata agttaatgga tgaacttcga tttctttgtc
    21001 tcctacttta aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga
    21061 tgcacccatt aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca
    21121 aacgctggct gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc
    21181 tgggatatct gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat
    21241 gcggatatct ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg
    21301 gtcaatcgtt acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa
    21361 gatataatct ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc
    21421 aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc
    21481 agcatctacg tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac
    21541 gattttatcg ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc
    21601 taggattggt gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc
    21661 catgcgatag ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat
    21721 gtcggcagct tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt
    21781 ttctaaatgt tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac
    21841 gctgtctaac agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt
    21901 tccagcaact agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg
    21961 ggcataccaa agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc
    22021 atctttatag ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt
    22081 tgcaggagaa gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc
    22141 tagtaaagat ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg
    22201 ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt
    22261 attcacagtg tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc
    22321 ttgaggcatg tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc
    22381 tttatagatt tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc
    22441 tttcgacaat gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag
    22501 ccctacatta cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat
    22561 attttaatat gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga
    22621 gggaatttac aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat
    22681 tttaggatgg gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa
    22741 cttactaaat tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata
    22801 accacaaact taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg
    22861 cggtcaccgt taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat
    22921 ggttcaattt ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca
    22981 acgttcggca ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt
    23041 ccagagaaca atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca
    23101 aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct
    23161 acttttttcg ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat
    23221 ttcactacat cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt
    23281 ttcttcggat caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc
    23341 ttcagcatca atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg
    23401 attaggtcta atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc
    23461 acatcgtatg ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac
    23521 tctgtaaatg agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg
    23581 ttgatgttga agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac
    23641 cagtcgtagt tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga
    23701 ttggaaagtg cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt
    23761 ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga
    23821 tggcctgcca attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc
    23881 gaatcaccag ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg
    23941 cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata
    24001 atattcattt tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt
    24061 gtaattaatt atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc
    24121 caagggacga aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct
    24181 cgaacattta acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct
    24241 ttaaagcatg tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact
    24301 acttcgtttc gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt
    24361 ttctttgtca acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc
    24421 cgctctaatt ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat
    24481 tttttctgca atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa
    24541 taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc
    24601 aaaaaattat tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt
    24661 tttttgtcat ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag
    24721 aaaaagcaaa agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg
    24781 ctcaaagcta cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat
    24841 ctactgaaac acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact
    24901 ggtctgatcc tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa
    24961 aagccttcca ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat
    25021 tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt
    25081 tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa
    25141 aaaaaccatt ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg
    25201 gttttcctag ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga
    25261 aagataaaga agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga
    25321 aacatcctga tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg
    25381 gagttttagg ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag
    25441 agattaaaga agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt
    25501 cccatacatg gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca
    25561 atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc
    25621 catttggtgc agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt
    25681 acttgaagag tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg
    25741 gacaattagg tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc
    25801 aatctgattt agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct
    25861 atgacaaaga agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga
    25921 actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc
    25981 ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg
    26041 gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt
    26101 tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac
    26161 tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt
    26221 atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt
    26281 cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt
    26341 cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc
    26401 caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg
    26461 tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt
    26521 tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta
    26581 aacttttata atatatatag ttcaaattta ttctgaggtt tattataact atctattgag
    26641 aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa
    26701 tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct ttttattaaa
    26761 ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa
    26821 tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct
    26881 ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat
    26941 ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt
    27001 ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag
    27061 gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc
    27121 ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc
    27181 ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg
    27241 ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt
    27301 tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc
    27361 tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtt gtgatcatac
    27421 gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga
    27481 ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg
    27541 actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga
    27601 ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg
    27661 aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt
    27721 cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat
    27781 ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt
    27841 ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg
    27901 tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg
    27961 aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc
    28021 aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac
    28081 gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa
    28141 tatcgatcag ttgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt
    28201 ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg
    28261 ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg
    28321 gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc
    28381 ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt
    28441 atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag
    28501 atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt
    28561 tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca
    28621 cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag
    28681 aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact
    28741 ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca attgctagca
    28801 tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa
    28861 tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt gctatattgg
    28921 caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga
    28981 aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag
    29041 tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt
    29101 atatctactt agcttactcc tttgcactca attactttgg cggaattttg gatttaccag
    29161 aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg
    29221 atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta
    29281 ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc
    29341 tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc
    29401 ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt
    29461 ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc
    29521 aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc
    29581 aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg
    29641 ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata
    29701 atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag
    29761 tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc
    29821 gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt
    29881 catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc
    29941 tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac
    30001 taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc
    30061 tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca actgataatt
    30121 ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct
    30181 ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt
    30241 ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa
    30301 ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt
    30361 ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat
    30421 ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt
    30481 caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag
    30541 ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc
    30601 tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt
    30661 ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca
    30721 ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt
    30781 ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag
    30841 tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt
    30901 gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg
    30961 tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat
    31021 cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc
    31081 tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat
    31141 tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca
    31201 gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta
    31261 gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct
    31321 gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct
    31381 gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct
    31441 tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg
    31501 tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat
    31561 gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct
    31621 cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat
    31681 tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg
    31741 gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca
    31801 ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga
    31861 ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct
    31921 ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc
    31981 ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa
    32041 atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag
    32101 cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct
    32161 ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc
    32221 tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca
    32281 tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac
    32341 gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc
    32401 atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg
    32461 atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga
    32521 gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac
    32581 ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct
    32641 ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt
    32701 tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc
    32761 aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc
    32821 atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac
    32881 cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa
    32941 taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac
    33001 tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa
    33061 aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac
    33121 tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac
    33181 atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt
    33241 ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag
    33301 aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct
    33361 tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag
    33421 gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt
    33481 gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg
    33541 atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc
    33601 acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa
    33661 agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta
    33721 ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc
    33781 gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt
    33841 tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt
    33901 gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa
    33961 actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct
    34021 gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt
    34081 tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat
    34141 cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt
    34201 ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat
    34261 tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt
    34321 tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat
    34381 tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt
    34441 catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc
    34501 tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga
    34561 aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac
    34621 tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca
    34681 ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat
    34741 gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt
    34801 atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat
    34861 tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt
    34921 tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct
    34981 tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt
    35041 gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa
    35101 ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa
    35161 cgttttctgt gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc
    35221 tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct
    35281 tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc
    35341 gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg
    35401 atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt
    35461 tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa
    35521 ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc
    35581 aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt
    35641 tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt
    35701 gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc
    35761 gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg
    35821 atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg
    35881 tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa
    35941 gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg
    36001 gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg
    36061 tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg
    36121 ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat
    36181 cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca
    36241 atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat
    36301 acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc
    36361 tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag
    36421 ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca
    36481 ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt
    36541 tgttacgacg taggagttta ccatacattt catttctttt cacataaagt gttcctaaat
    36601 ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt
    36661 ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa
    36721 tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc
    36781 catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca
    36841 aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg
    36901 tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca
    36961 aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt
    37021 cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt
    37081 tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga
    37141 tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa
    37201 agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca
    37261 atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg
    37321 ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat
    37381 caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt
    37441 caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat
    37501 attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa
    37561 tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca
    37621 cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt
    37681 tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt
    37741 cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg
    37801 gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca
    37861 aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac
    37921 cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat
    37981 ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg
    38041 atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa
    38101 tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt
    38161 gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct
    38221 tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta
    38281 cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt
    38341 taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat
    38401 tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat
    38461 ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta
    38521 caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt
    38581 ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg
    38641 aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt
    38701 ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt
    38761 ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg
    38821 tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt
    38881 ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca
    38941 ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac
    39001 tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat
    39061 tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg
    39121 gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt
    39181 ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca
    39241 atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt
    39301 gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt
    39361 gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc
    39421 tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag
    39481 cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct
    39541 gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt
    39601 ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct
    39661 tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt
    39721 acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct
    39781 actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca
    39841 ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt
    39901 cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact
    39961 ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga
    40021 atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc
    40081 atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat
    40141 gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct
    40201 gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg
    40261 tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag
    40321 atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct
    40381 ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag
    40441 ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc
    40501 attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg
    40561 attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac
    40621 attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc
    40681 tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg
    40741 taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac
    40801 atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt
    40861 tattgattga aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca
    40921 tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt
    40981 cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag
    41041 aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga
    41101 gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat
    41161 cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga
    41221 taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact
    41281 agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt
    41341 tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa
    41401 aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc
    41461 tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca
    41521 atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac
    41581 cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt
    41641 agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa
    41701 gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta
    41761 ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg
    41821 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta
    41881 agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata
    41941 ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt
    42001 cgagtattaa gaaaaatttt gtaaaaatgg atttctacat ttttgcataa atttggctta
    42061 ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg
    42121 acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc
    42181 taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta
    42241 actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct
    42301 tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc
    42361 acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa
    42421 gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat
    42481 tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca
    42541 taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac
    42601 ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat
    42661 gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc
    42721 cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa
    42781 cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga
    42841 taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct
    42901 ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt
    42961 ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca
    43021 gacactgata ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc
    43081 tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc
    43141 gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt
    43201 aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt
    43261 ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta
    43321 acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt
    43381 cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg
    43441 tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat
    43501 ccattttatg agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga
    43561 tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga
    43621 tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc
    43681 tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca
    43741 ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg
    43801 atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg
    43861 acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc
    43921 ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct
    43981 ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat
    44041 ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat
    44101 ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg
    44161 atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata
    44221 gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac
    44281 tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta
    44341 tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc
    44401 gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga
    44461 tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg
    44521 cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg
    44581 tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag
    44641 ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa
    44701 tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc
    44761 agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag
    44821 cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc
    44881 atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg
    44941 atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa
    45001 gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct
    45061 taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc
    45121 gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac
    45181 cagctacact cacaaaacat accccattct tctaggtctt atttattttc cacttaacta
    45241 tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg
    45301 aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat
    45361 atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac
    45421 caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg
    45481 ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa
    45541 aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg
    45601 gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg
    45661 ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta
    45721 aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca
    45781 tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag
    45841 ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt
    45901 tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg
    45961 tcccaataaa ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta
    46021 gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct
    46081 cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat
    46141 caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg
    46201 ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac
    46261 caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa
    46321 aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta
    46381 attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt
    46441 ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca
    46501 atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca
    46561 gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt
    46621 catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt
    46681 tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc
    46741 cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg
    46801 cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca
    46861 catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt
    46921 aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc
    46981 gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat
    47041 ctacaactaa atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat
    47101 tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga
    47161 tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat
    47221 catataaaaa gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct
    47281 ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg
    47341 cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca
    47401 gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg
    47461 ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa
    47521 gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa
    47581 ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa
    47641 gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt
    47701 tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga
    47761 tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg
    47821 aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa
    47881 ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct
    47941 tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa
    48001 aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag
    48061 atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat
    48121 acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa
    48181 gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca
    48241 aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga
    48301 acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct
    48361 ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata
    48421 cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa
    48481 ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca
    48541 taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg
    48601 tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa
    48661 ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa
    48721 tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt ccgactggca
    48781 ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg
    48841 atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca
    48901 agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg
    48961 tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg
    49021 gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt
    49081 gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg
    49141 atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc
    49201 acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt
    49261 cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata
    49321 tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc
    49381 ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt
    49441 tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct
    49501 attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca
    49561 cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca
    49621 taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa
    49681 gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata
    49741 taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta
    49801 tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac
    49861 tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag
    49921 acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct
    49981 actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc
    50041 cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga
    50101 taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt
    50161 tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt
    50221 gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta
    50281 cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct
    50341 gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt
    50401 tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca
    50461 taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat
    50521 ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg
    50581 atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg
    50641 agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca
    50701 tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc
    50761 atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt
    50821 tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc
    50881 aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt
    50941 attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc
    51001 atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag
    51061 aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc
    51121 aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta
    51181 tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc
    51241 taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg
    51301 tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga
    51361 aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata
    51421 aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa
    51481 tgttaattgt tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag
    51541 atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga
    51601 cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg
    51661 ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg
    51721 gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc
    51781 aataattgca attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat
    51841 ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga
    51901 accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt
    51961 tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt
    52021 cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat
    52081 caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc
    52141 acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc
    52201 gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct
    52261 caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc
    52321 agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg
    52381 acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga
    52441 aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg
    52501 agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta
    52561 atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa
    52621 gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt
    52681 tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct
    52741 cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat
    52801 ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc
    52861 gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc
    52921 cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta
    52981 aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt
    53041 catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa
    53101 aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc
    53161 acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata
    53221 ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa
    53281 attatataat gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac
    53341 aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg
    53401 ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg
    53461 gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag
    53521 aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac
    53581 atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa
    53641 acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt
    53701 tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata
    53761 acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa
    53821 ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat
    53881 cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat
    53941 tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg
    54001 ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc
    54061 ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc
    54121 atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc
    54181 agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt
    54241 gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt
    54301 caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc
    54361 cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg
    54421 tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat
    54481 tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat
    54541 gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat
    54601 aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt
    54661 caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg
    54721 gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg
    54781 tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa
    54841 agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg
    54901 cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc
    54961 ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga
    55021 caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt
    55081 atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
    55141 gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca
    55201 agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg
    55261 ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc
    55321 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt
    55381 gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt
    55441 ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt
    55501 ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct
    55561 tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata
    55621 tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc
    55681 atctgctcaa tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct
    55741 tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac
    55801 ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat
    55861 tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct
    55921 tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa
    55981 tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct
    56041 ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta
    56101 aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa
    56161 tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca
    56221 tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc
    56281 gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt
    56341 tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg
    56401 aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca
    56461 tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc
    56521 ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt
    56581 gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta
    56641 aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg
    56701 tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc
    56761 gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac
    56821 acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc
    56881 gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac
    56941 ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac
    57001 tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca
    57061 aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt
    57121 gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc
    57181 cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt
    57241 ttacagaact gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc
    57301 cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag
    57361 tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa
    57421 aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc
    57481 cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg
    57541 ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg
    57601 gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg
    57661 tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc
    57721 caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac
    57781 gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa
    57841 tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat
    57901 aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa
    57961 ctaataaaat aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa
    58021 tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta
    58081 aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc
    58141 gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa
    58201 taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata
    58261 taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt
    58321 cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc
    58381 ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta
    58441 aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt
    58501 gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc
    58561 ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct
    58621 tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat
    58681 tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta
    58741 cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag
    58801 aaaacggttt ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt
    58861 gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt
    58921 ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa
    58981 gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc
    59041 acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt
    59101 gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat
    59161 caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat
    59221 aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact
    59281 caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt
    59341 gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt
    59401 gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta
    59461 tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc
    59521 tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa
    59581 tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg
    59641 catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca
    59701 ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct
    59761 tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat
    59821 tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt
    59881 ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt
    59941 cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg
    60001 aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga
    60061 tattggaaaa aattgtactg atttgttgag gcaaccttgc gtgttggatc accgtttctg
    60121 ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt
    60181 ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt
    60241 ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac
    60301 tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc
    60361 tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa
    60421 ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga
    60481 tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag
    60541 ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt
    60601 gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac
    60661 ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat
    60721 tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact atcttttttc
    60781 aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc
    60841 ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca
    60901 atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac
    60961 tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg
    61021 ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa
    61081 cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag
    61141 ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag
    61201 acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt
    61261 tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa
    61321 gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc
    61381 agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag
    61441 ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg
    61501 gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca
    61561 atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata
    61621 cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt
    61681 tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc
    61741 ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg
    61801 atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt
    61861 ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct
    61921 tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc
    61981 gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct
    62041 tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta
    62101 ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc
    62161 cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa
    62221 tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga
    62281 agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata acaaatacat
    62341 aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc
    62401 caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg
    62461 ataatttgac acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact
    62521 ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac
    62581 atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct
    62641 acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc
    62701 ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa
    62761 cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc
    62821 cttgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat
    62881 attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc
    62941 taactcttct ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa
    63001 catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg
    63061 aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca
    63121 tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt
    63181 gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct
    63241 tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt
    63301 catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt
    63361 aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat
    63421 acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg
    63481 aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata
    63541 ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt
    63601 aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt
    63661 atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc
    63721 catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat
    63781 cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt
    63841 cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc
    63901 caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact
    63961 tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct
    64021 cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa
    64081 attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa
    64141 acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg
    64201 ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa
    64261 aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac
    64321 gctgatttca caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa
    64381 gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat
    64441 tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc
    64501 gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa
    64561 agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac
    64621 aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc
    64681 tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac
    64741 aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt
    64801 ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa
    64861 agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg
    64921 aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg
    64981 tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt
    65041 tgtagctgcc atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt
    65101 tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac
    65161 taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa
    65221 tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat
    65281 ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc
    65341 taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt
    65401 gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca
    65461 gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc
    65521 acctaataca gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc
    65581 tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat
    65641 tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt
    65701 tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa
    65761 aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa
    65821 gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc
    65881 attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt
    65941 tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga
    66001 ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct
    66061 tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca
    66121 ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg
    66181 ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt
    66241 tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt
    66301 ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct
    66361 aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca
    66421 gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca
    66481 taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt
    66541 tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact
    66601 attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca
    66661 aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc
    66721 tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt
    66781 ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc
    66841 aatgaccagc accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca
    66901 gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc
    66961 cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa
    67021 atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa
    67081 ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt
    67141 tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt
    67201 aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta
    67261 atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt
    67321 tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac
    67381 ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat
    67441 aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg
    67501 ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa
    67561 acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt
    67621 aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct
    67681 aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc
    67741 gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct
    67801 gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta
    67861 tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct
    67921 gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt
    67981 ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa
    68041 agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag
    68101 tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt
    68161 ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat
    68221 ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct
    68281 tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa
    68341 tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg
    68401 tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct
    68461 ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag
    68521 taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt
    68581 tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt
    68641 ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac
    68701 cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt
    68761 gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt
    68821 catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag
    68881 gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga
    68941 tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt
    69001 ttatttcttc gaaagtttct tttcctattt cactgattag ttccaatgaa atcgtccgga
    69061 tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa
    69121 gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag
    69181 caaatgtatg actatgtaat caagtataga agactggttt aattacggca aataaatata
    69241 atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt
    69301 ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca
    69361 aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc
    69421 tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca
    69481 atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa
    69541 cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc
    69601 atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta
    69661 atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc
    69721 atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt
    69781 cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg
    69841 atctgattca cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag
    69901 gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg
    69961 cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc
    70021 aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa
    70081 attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata
    70141 catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact
    70201 accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt
    70261 ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg
    70321 gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta
    70381 ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga
    70441 tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact agggggacat
    70501 caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt
    70561 gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt
    70621 tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt
    70681 ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt
    70741 ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca
    70801 tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt
    70861 ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta
    70921 acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac
    70981 ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag
    71041 ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca
    71101 tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt
    71161 tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga
    71221 gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc
    71281 gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat
    71341 tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta
    71401 gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc
    71461 gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa
    71521 aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc
    71581 caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgatgaat
    71641 cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga
    71701 gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct
    71761 gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg
    71821 ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca
    71881 gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact
    71941 aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca
    72001 gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata
    72061 tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg
    72121 atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa
    72181 tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc
    72241 ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca
    72301 tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa
    72361 caagctccga taaaatcacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag
    72421 ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac
    72481 ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt
    72541 gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa
    72601 gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt
    72661 ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg
    72721 ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat
    72781 gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg
    72841 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
    72901 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
    72961 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
    73021 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
    73081 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
    73141 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
    73201 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
    73261 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
    73321 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
    73381 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
    73441 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
    73501 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
    73561 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
    73621 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
    73681 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
    73741 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
    73801 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
    73861 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
    73921 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
    73981 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
    74041 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
    74101 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
    74161 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
    74221 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
    74281 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
    74341 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
    74401 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
    74461 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
    74521 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
    74581 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
    74641 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
    74701 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
    74761 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
    74821 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
    74881 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
    74941 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
    75001 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
    75061 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
    75121 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
    75181 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
    75241 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
    75301 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
    75361 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
    75421 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
    75481 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
    75541 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
    75601 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
    75661 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
    75721 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
    75781 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
    75841 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
    75901 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
    75961 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
    76021 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
    76081 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
    76141 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
    76201 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
    76261 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
    76321 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
    76381 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
    76441 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
    76501 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
    76561 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
    76621 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
    76681 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
    76741 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
    76801 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
    76861 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
    76921 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
    76981 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
    77041 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
    77101 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
    77161 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
    77221 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
    77281 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
    77341 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
    77401 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
    77461 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
    77521 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
    77581 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
    77641 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
    77701 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
    77761 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
    77821 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
    77881 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
    77941 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
    78001 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
    78061 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
    78121 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
    78181 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
    78241 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
    78301 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
    78361 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
    78421 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
    78481 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
    78541 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
    78601 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
    78661 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
    78721 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
    78781 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
    78841 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
    78901 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
    78961 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
    79021 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
    79081 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
    79141 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
    79201 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
    79261 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
    79321 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
    79381 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
    79441 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
    79501 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
    79561 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
    79621 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
    79681 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
    79741 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
    79801 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
    79861 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
    79921 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
    79981 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
    80041 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
    80101 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
    80161 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
    80221 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
    80281 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
    80341 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
    80401 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
    80461 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
    80521 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
    80581 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
    80641 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
    80701 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
    80761 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
    80821 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
    80881 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
    80941 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
    81001 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
    81061 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
    81121 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
    81181 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
    81241 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
    81301 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
    81361 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
    81421 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
    81481 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
    81541 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
    81601 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
    81661 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
    81721 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
    81781 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
    81841 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
    81901 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
    81961 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
    82021 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
    82081 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
    82141 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
    82201 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
    82261 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
    82321 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
    82381 cgacaacttc gttgacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
    82441 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
    82501 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
    82561 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
    82621 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
    82681 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
    82741 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
    82801 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    82861 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    82921 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    82981 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    83041 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    83101 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    83161 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    83221 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    83281 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    83341 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    83401 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    83461 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    83521 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    83581 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    83641 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    83701 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    83761 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    83821 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    83881 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    83941 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    84001 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    84061 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    84121 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    84181 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    84241 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    84301 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    84361 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    84421 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    84481 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    84541 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    84601 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    84661 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    84721 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    84781 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    84841 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    84901 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    84961 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    85021 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    85081 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    85141 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    85201 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    85261 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    85321 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    85381 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    85441 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    85501 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    85561 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    85621 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    85681 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    85741 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    85801 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    85861 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    85921 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    85981 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    86041 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    86101 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    86161 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    86221 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    86281 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    86341 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    86401 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    86461 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    86521 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    86581 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    86641 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    86701 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    86761 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    86821 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    86881 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    86941 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    87001 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    87061 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    87121 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    87181 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    87241 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    87301 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    87361 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    87421 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    87481 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    87541 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    87601 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    87661 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    87721 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    87781 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    87841 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    87901 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    87961 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    88021 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    88081 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    88141 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    88201 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    88261 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    88321 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    88381 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    88441 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    88501 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    88561 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    88621 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    88681 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    88741 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    88801 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    88861 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    88921 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    88981 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    89041 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    89101 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    89161 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    89221 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    89281 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    89341 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    89401 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    89461 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    89521 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    89581 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    89641 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    89701 gtttacaata catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa
    89761 ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt
    89821 ctgaaataat tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc
    89881 ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg
    89941 tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat
    90001 ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca
    90061 agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa
    90121 acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg
    90181 taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca
    90241 aacttttttc ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg
    90301 ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat
    90361 ggatcacttg cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa
    90421 caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac
    90481 agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga
    90541 tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa
    90601 agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg
    90661 tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga
    90721 tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga
    90781 ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc
    90841 agaaatggat atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa
    90901 gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat
    90961 cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac
    91021 cataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa
    91081 ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact
    91141 ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt
    91201 tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata
    91261 cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat
    91321 gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg
    91381 acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc
    91441 aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata
    91501 cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc
    91561 agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg
    91621 gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta
    91681 gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca
    91741 ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg
    91801 caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca
    91861 tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat
    91921 actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt
    91981 tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta
    92041 gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca
    92101 actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct
    92161 aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg
    92221 atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc
    92281 acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca
    92341 ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca
    92401 ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag
    92461 atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc
    92521 cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt
    92581 ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct
    92641 tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac
    92701 aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta
    92761 gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag
    92821 aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa
    92881 ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg
    92941 gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa
    93001 aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg
    93061 ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc
    93121 accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc
    93181 gccatccata taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa
    93241 atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa
    93301 tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct
    93361 gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa
    93421 tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc
    93481 caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc
    93541 gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata
    93601 agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct
    93661 gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc
    93721 aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga
    93781 acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt
    93841 taacctttct cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt
    93901 tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt
    93961 tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag caaccatctg
    94021 atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg
    94081 gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac
    94141 ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa
    94201 attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc
    94261 taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc
    94321 atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc
    94381 tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt
    94441 ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg
    94501 tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac
    94561 ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc
    94621 tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc
    94681 atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat
    94741 aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat
    94801 gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa
    94861 tttttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    94921 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    94981 atagataacc taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat tcactaaaca
    95041 tatttttatt tttttatatt gttaaaaatt cgcaatttat tttttaatag aattatctgt
    95101 tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa
    95161 ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat
    95221 acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct
    95281 gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct
    95341 cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga
    95401 agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca
    95461 tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg
    95521 tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat
    95581 ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg
    95641 gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct
    95701 taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga
    95761 acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga
    95821 aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc
    95881 tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt
    95941 cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt
    96001 tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga
    96061 agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt
    96121 tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc
    96181 gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt
    96241 ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag
    96301 ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt
    96361 gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac
    96421 aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga
    96481 ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca
    96541 gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga
    96601 aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa
    96661 caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt
    96721 tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg
    96781 ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct
    96841 agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca
    96901 agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg
    96961 ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt
    97021 gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt
    97081 ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc
    97141 tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga
    97201 taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga
    97261 tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc agttttcttg
    97321 tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa
    97381 gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa
    97441 tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact
    97501 ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta
    97561 aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct
    97621 ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta
    97681 gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct
    97741 aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac
    97801 aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat
    97861 ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt
    97921 tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc
    97981 tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt
    98041 cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga agacattgga
    98101 ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca
    98161 gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca
    98221 ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg
    98281 aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt
    98341 aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag
    98401 ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac
    98461 aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat
    98521 aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa
    98581 ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac
    98641 ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg
    98701 cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc
    98761 aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa
    98821 gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca
    98881 ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa
    98941 gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca
    99001 gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca
    99061 gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa
    99121 ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat
    99181 ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca
    99241 tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctatcgtc
    99301 gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat
    99361 ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga
    99421 aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat
    99481 attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg
    99541 ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct
    99601 aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc
    99661 tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt
    99721 cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt
    99781 gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta
    99841 ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg
    99901 cagatgcaag cttttgaaca ccgtttacca agaggttgtg aaaaagctgc cctgcatcaa
    99961 gtaataagaa cacccaagct aaaactgctt cttatgtttt tgacttggtt ggacttatta
   100021 ccgcagatgc aagcttttga acaccattta ccaagaggtt atgaaaaagc tgccctgcat
   100081 caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta
   100141 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag
   100201 tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac
   100261 ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt
   100321 ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat
   100381 acaactattt caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca
   100441 aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt
   100501 aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc
   100561 tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca
   100621 gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa
   100681 cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag
   100741 ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa
   100801 cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt
   100861 tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg
   100921 ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa
   100981 gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa
   101041 aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag
   101101 taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa
   101161 aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc
   101221 tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag
   101281 ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag
   101341 gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg
   101401 gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag
   101461 taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg
   101521 atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa
   101581 tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag
   101641 aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt
   101701 tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa
   101761 atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc
   101821 gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga
   101881 ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct
   101941 atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga
   102001 acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat
   102061 gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg
   102121 agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata
   102181 aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt
   102241 tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa
   102301 cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc
   102361 cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc
   102421 cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt
   102481 tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca
   102541 gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca
   102601 agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa
   102661 tcacttgttc tccgtttttt gattgatcaa gcatctaaag cttcttttct tctttcttgg
   102721 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
   102781 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
   102841 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
   102901 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
   102961 ataggtaacc caaacatcgt tggttcatta atattgaagg acacctggcg caatcgacaa
   103021 gtttgttaca accatatatg gtttgttttt acgtcctact aaataaatag ctaacagtaa
   103081 acctaaagtt gcacctgttc ctcctaagtt gacaaagctg tcaaagaaag gtttgttcac
   103141 gataaacggt gcgctatgcc cagctgcgat tgcattaacg tttgcttcaa tggctggcgc
   103201 attgattgtt tgcatcaaag gatcgaccat attcgctccg tgtaaaccaa agaaccataa
   103261 gaatggtgtg ataaatgcaa gcagtaaggc agatggataa gagtttgcaa gtcccataaa
   103321 tggttcttgg accactgtat agaaagaacc tacgatatca tcaacaccga atgcagcaaa
   103381 aattgcagca actaaagcaa acgcagagat agtgatcatt gctggtagta atgaagcaaa
   103441 tgcttttgca actgccggtg gcacaccatc aggcattttg atgatcaatt tttcatttcc
   103501 gcttaaacgt acaaagattt ctgttgaaat aatcccaaca ataatagcaa taaacaaacc
   103561 gtttgagctc atacctaatg ctccgcctaa agcgaagaaa gagcctaaag aaatcacacc
   103621 tgcggaaaca ccgtcttttc ggtagccgtt agctaaatga taagcaagca agaaagcaat
   103681 caagacagaa agaatggcaa atgtaccgtt ccaagcagca gccccgaatc ctttccatga
   103741 ttcaccacca aaaattgagt tcatcaattc ttggaacccg ggaatcggta agttattgat
   103801 cattactgct aatgctccta agatcattaa cggcatactc accataaatg agtcccgaat
   103861 cgcaactaaa tgccgttgtg caccaatttt tgatgctaca ggtataaaat gtttctccat
   103921 aaatgcaaca aatttatcca taaatcttcc ctcttttctt tactgtagat tacgcgttca
   103981 atcgacgata cagatcaatg atctcttttg ctaaatctgt aaaagctata gatgtcatca
   104041 gatgatcttg actgtggacc gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt
   104101 gtgtcagcat ctctgtttga gagtgatgtg cttggactaa agattcctct gcatctttga
   104161 ttttttcatc tgctaaagtg aaatcacctt ttttcgcagc agcaatcgct tccattgcat
   104221 cgctttttgc atttccgcca aacatgatca gacccatgat ggcttctaga tttttttgat
   104281 cttccattta ttgtatcctc cgataatttt tattatttgt ttattagttt ttctgcttgg
   104341 tcgagtactt tttcaccatt catcatgcca tagtcagcca tattgattac atctaaagga
   104401 atgcctttag gtgctagctt ttcagcaaat tgattttgca taaagcgtac ttgcggtcct
   104461 aaaagtaaaa cgtctacatt tttagattct agttgattat ccgcatctga agcagataca
   104521 gcaaagatgt ctgcttccat tccttttgct tctgctgcct tttgcatttt tgtgactagg
   104581 agacttgtac tcattccagc tgaacataca agcatgattg ttttcttttc cattttaaac
   104641 acgttccttt ctatacttac acttgtatat aagcaagtag cgtgccaact ttaaaagcgc
   104701 tttaaaaagt tgatatcatc acatttaagg tctataccaa gttgcataca cactaaaaaa
   104761 gaaagcgcac actaaacaca caaattaatt tttattacct tttttctttt aacgtgcaca
   104821 aaaaaagttg tgacacaaaa cttgtcacaa cttttttcaa ataaacggta ttcaaaagct
   104881 tgcatctgcg gtaataagtc caaccaagtt aaaaacataa gatgttgttt ttactttgct
   104941 attcttatta cttgatgcag gacagctttt tcacaacctc tcaataaacg gtgttcaatc
   105001 agctgaccct gggtattagc tcaaaacgct ggattcacaa cctcttttat ttcaaataac
   105061 cagtaaatac aatgaacatc tacaaaaaac ttctatttat ttatagattt cgtcaggcaa
   105121 atccaattct ctcttatcaa tcaaagtctg ataccacgaa gcagattttt tcaatgttct
   105181 gcttcgttca tcgttcaatt cgattcgaac taatccatat cgattcttaa atgcattcat
   105241 tggtgacaca cagtcagtga atgcccataa catatagcct tcgcaatttg cgccttcttc
   105301 tgttgctttg atcaagtaat gcaaatgctg tccgataaat tcgatacggt aatcatcttg
   105361 cacgattcct ttctcatcca tatactgttc ttcatattct cttcccatac cgttttctgt
   105421 tatcagccaa gggatattat gataattttc ttttagatac atggctatat catacatgat
   105481 tttcggatag atttcccatc ccctggaatt attcattttc ttcccaggta aatcaaagac
   105541 ttcataatat cgttcaggat ggaaaggtgt ctcttcattc cattgataac gaggggcgcg
   105601 aacgcgttta ggataatatt gattgattcc aaggaaatct actgtatgat gctgaatcgt
   105661 ttctaaatcc tctgttgcag gatcgaaata aatcgattgt ttttgacaca actccaggaa
   105721 ctcttctgtg tagtatcctt ttaccatcgg atcaaaaaat actcgattat aaaacaaatc
   105781 atacatataa gcagcttttt ggtcttctgg agaattggat cgagcataga ccatttcagg
   105841 atttaaaata cagccaatcc ttccaccata atttccgctg cggtaaatct ctactgcttt
   105901 agccgttgct aaaactttat gataattcca catcatccac ttttttgtgt tttgttcatg
   105961 cggccagcga acagcatcca aatagcaacg cgtttgggga acgattggtt cgttgaatgt
   106021 aaaccagttt tttaccctat caccgtaacg atcaaaggca atttgagcat acttagcata
   106081 aaaatcaact acttttcttg aagaccagcc atcatatttt tccaaaagaa aagctgggac
   106141 ttcataatgt tccaaacaaa tcatcggttc tacgtctgct tcgatcaaag agtcgatcat
   106201 ttcacttata tgctgagcat attcttcatc tactatacag ttctcataat cagtaaaaaa
   106261 acgagcccaa ttgatagaag tccgataatg tgtcagatgg atggatttca tcagatccac
   106321 atcctgctgg tattgttcca taaaattagt tgctacggct gggccatacc cattatgcca
   106381 aacaaaatgt tcatttttat accagctatc tataaacgaa tcctgtcctt ctttttttcc
   106441 tttccaacct tccgtttgcc aagcagaaga tgcagcacca agaataaaat tttttggaat
   106501 agtaattttc atgatttcct cctgtttttt agatcccatc attttccaca cgatttgcta
   106561 cttttacaaa tggcagatag atcaaaatag aaacaataat acaaacaagt tgcgtaataa
   106621 ctgcaccgat atttcctgct gttgccagat aagcattgac gatcggcggc attggccaag
   106681 ggaccattac aactgctttc gccgcaaatc ctaaagcggt tgcaaaataa ccaatcgttc
   106741 cagtgataag aggcgttaag ataaacggaa tagctaaaat tggattcagc atgatcggca
   106801 taccaaaaat aactggttca ttgatgttga agatcccagg agcaagggat aacttggtga
   106861 tttgtttcat atcttcccgt tttccaacta acaatactgc aatcagtaaa ccgatcgtga
   106921 ctccagaccc gcccatactc atatacatat cccaaaatgg catagtaata atgttcggta
   106981 cttcttttcc agcttcaaaa gcttccgtat ttacagcaat tgccgctaaa agcaacggtt
   107041 cccgtatcgg cttcaccatt tgatttccat ggataccaat cacccaaaag acttgcgcaa
   107101 taaacatcaa tagcaaaata cccggtaacc cttgtacgac accttctaat gggcgttgta
   107161 cgacattgta aatgatatca taaaggtaca ttcctgtaag ttctttgatg ataaatcccg
   107221 cagccgcgat acccgataca gtcagaatcg tcgggaataa agcagaaaaa ctagcagaaa
   107281 cattcgttgg cacggtatca ggcattctca gctgtagttt cttctgtttc ctcaaccagc
   107341 aataaagttc aatagaaagg atggagacca acattcccaa aaataaccct ttcgtatctg
   107401 tatattgttt agccaatacg ttttctacta taacagtctg attttcattc actaattcat
   107461 aaacagttgg attaacagat aaataagaca tcactgcgat gataccaggg aatacaccat
   107521 ccactttatt cagcttggct aattcgatcc ctatcagaaa aacagcatag atggtcaaga
   107581 aacttagtgt gacatagctg atggcagatg cgataggctt taattcttct aaaaaacgca
   107641 agaaacttat ctgtgctaag ccattttccg agtcaaagac cattgctgaa aacaatgtgc
   107701 caaaagcacc cgtaataatt accggaatca atgcagcaaa tgcgtttttt atcaccatga
   107761 tataccttaa attattgatt ctcgttgcca cgttccctaa aacattggct aatttatcca
   107821 taaaactcat tccagatccc ctcattcctg cttcttctcc ttgacgtcat ctctttttgt
   107881 ctattagtca gcgcttacat ttcgatgaac agaaactatt tactcctctt ttttaccaaa
   107941 tataaaacaa aaattcatct gatgtattta tcataaaata catcagatga actgtcaatt
   108001 agtttctttt tctcctatta ttttctgtaa ttctctccga tttattgcca tatggacagt
   108061 cattgcatca aacgcagcaa gtgcattccg cctttcgatg gcctgaacga tctctgtatg
   108121 aatatcgatc gtctcgattt tcagatgctg gatatcataa tttttattaa acaaagcaat
   108181 tgattcatta atgattggga taatatgcag caaggctacg tttccgctag cttctgcaat
   108241 catcgtatga aaatcgatgt ctaattgaac atgttcttgt gtgccttctc gaaaagaagt
   108301 ttcaatcttt tgcgttaatt ttttcatttg gacgatttga ttcttcgttg catattccgc
   108361 tgccaaacct gccatcttag gctccaataa atatctcagt tcaaataagt ctcgtatcat
   108421 ccgatcctga tcttcaatca aagaaaaacc gagtgggtca ttggcaatcc ctcgcttatc
   108481 actgacaaat gtccccgctc cttgccgaac ttcaaggata ttcctggaag ctagagcccg
   108541 cacagcttct cgcaccgtgc ttcgccccac ctctaaataa tcggacaatt catattcatt
   108601 cggcagttta tctcctactt gcaactgcat ttcctttaaa tatctaagga tcttgtcagt
   108661 agttacttct actagggttt tattttcttc caaaccacat acctcctttt catctgatgt
   108721 attttttcgt aattattata acacgtttga atcatctgat gattttttgt atacaaaatt
   108781 gattaatccg ttcatagaca tacaaacgaa gaagatattt accataaaaa agactaggat
   108841 aaaattcact cctagccttc gatcattttt attttgaacg acaaaaaata tgatgttcaa
   108901 taacagacga tcttcattcg attcttgatg tcatcttttg ttttttttaa tatatggata
   108961 aaaagtttga ttacatcgtt tctattctag aatttggtcg attctcaatt cttcttctgg
   109021 tgagaaatct aaattatcca tcattcgaac actttcttgt aactgactta accgacttgc
   109081 tccgaccagt acacttgtaa cagatttttg tcgcaaattc catgcaaggg ccatttgcgc
   109141 taatgattgt cctcgtgata cggcgattgt ttgcagtgct ttgatttttt ccagagttga
   109201 tccaacttgt tcttctgaaa ggaatggtat ttccttacga tgtgctctag aatcttcagg
   109261 aataccatgt agataacgat ttgtcagcaa cccttgagca agcggactga acgtgattgc
   109321 ccctagccct tcttcttcta atactggaga tagatcgtct tctagtagtg cacgagaaaa
   109381 catattgtag cgcatctgat gaataatgaa cggtgcttcc tttcttttta aaatttcagt
   109441 catttttttt gtatcttctc cattatagtt ggatatacca atatataatg cttttccttg
   109501 tcttacaagt tgcatcaaag cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg
   109561 atgatgatag aagatgtcaa cataatctag ccccattctt tggagacttt ggtcacaact
   109621 tgaaatgatg gatttacgag agccccattc cccatatggc ccttcccaca tatgataacc
   109681 tgctttgcta gaaataatca gttcatcacg gtaggcttga aaatcttctc taaaaatacg
   109741 cccaaagttt ttctctgcag ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg
   109801 ggtaattccc atgtcgaatg cgcctcttaa aatctctctt tgattatgga taggatcata
   109861 atcaccaaaa ttttgccata atccgagaga taaaatcggt aatttcaaac ctgaatgacc
   109921 ggatttacga tattccattt tttcataacg tgcactatct gcaatataca atttttctcc
   109981 tcctcatcaa atctttttaa aacgctttct tttttctatt tttagtttac tacattttaa
   110041 agaaaaggag ttaatcaaaa tgattttttc gtatattttt ttgccatttg ttttttactc
   110101 gttgatagat cagaaattga ttacgtgctt gctggatttt tttataacgt ttcggttgtt
   110161 ttttgtaaaa ttgctgatga tagttttctg ctggccaaaa cgtaccggct ggttcgattt
   110221 ttgtgacgat tggttggtgg aaagttcctg aactgtctag tttctgtttt gattgttccg
   110281 ctaattcttt ttgtcgttcg ccttgataaa aaatcactgg acgatattgc ttccctctat
   110341 cttgaaattg tccaaatgta tctgtgggat cagtaatttg ccaatagatt gtcagcaact
   110401 cgctataact tatttctctt gtgtcataaa tgatctccac agcttccaca tgacctgtgt
   110461 aaccaccact tacttgatca taatttggtt tttcaacatg tccgcccgtg tatcctgaca
   110521 aaacagatag gatccctttt ttcgattcaa acggctcgac catacaccag aaacatcccc
   110581 ctgcaaatac tgcacgttct tgatagtcag catcttgcat ggcatgcttg gaaaagtcat
   110641 atttttgctc cttatcaaat ccttccgtaa tcaataaata aaagtccatt acatcaggcg
   110701 tcagattcat tcggatagct aatggacgta gtgttgcttc tagttttgat aactgttctt
   110761 taacggatat attttctctt atgtcattct ttgtctgaat caatactttt cgttcccaat
   110821 ctcgtgtatt cggattcaaa atcagattat aaagtgtttg aatgaatatt tgttcctcat
   110881 ccatagattc tcctcctttc cgaagaagac aagctagtga ctttagctgt aatttatcat
   110941 aaagtgagga aataagcgat tacttcatcc ccctacagat ttttcgtata aaaaagagag
   111001 aatccttatc gacagattct cctttttaat caatttattt tataaatgaa aagttaattt
   111061 accttttatg gaaaactatt ttaaaaaata aacataaata tgcagttaat gtaacaagat
   111121 aagaaataat agtcgttact gtataacttg tgcttacttt aatatcagca actcctttga
   111181 ctccgcttat ttgtaccaat ccattagcat tcacctgctc ctttacaggt tgatgattta
   111241 tttctacatt cgttcctaaa taagcatata caggaagtgt gattacttgg ctcttctcat
   111301 tagttactct atatatgata gaactaccag ttgttttata aggaaggttt acccattgac
   111361 tgccaatctt tgcttcgtgt tttattatat gctccttatt ctccaatgaa gcgataggag
   111421 aataatcata taaaaaataa tccattcctt tagtggtata cgcctctact gtttctttgt
   111481 ttacaacaat atgtcccggc tgagaaatta ttttttgatt gaaatttttt ttactagaaa
   111541 cagttaaagt gattaataac aaaacagtca taaataaata aaatagttct cttgtcttat
   111601 tgtatcttct atttttgaaa aatatcatcg ttaatatcag agaagagaat attatttgca
   111661 agcttaagat ccgccaagga aattgtaaca atttggctgg cgtattttgc agaagatacc
   111721 atggcaccaa actagttgta gctagccaag tcaaacacgt accataataa atcttttttt
   111781 ctatgtttgt taatttttta aataaaatca ggggaataca taaagataac agaccaacta
   111841 gtcccattga gtaagtagtt aagtcgttgt tcatagcttc agtcagacta tcgaacacat
   111901 tagatgcatg agcatataaa tttgtcacgc taggtttgtt gattttttgg tagagcattt
   111961 gttcaaacat cggataccaa aaagcactcg tcagaaccaa tgtcattcct gccgcactaa
   112021 ataatttttt aaatatagca ataaaatctg cattcttgaa acctttagaa accaaacgaa
   112081 taaaaatgat aattaatgct gtactcatta atgttgatag tacatggctg tacactagca
   112141 aactcatact caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt
   112201 gataagcata aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg
   112261 aacgatagta gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa
   112321 atgccgcttt tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac
   112381 acaagttcaa taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat
   112441 ataacattac agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag
   112501 tataattgaa tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc
   112561 gagataagtg aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga
   112621 tgaaagaata tatactacta actataatta atccactcaa tattactact tttttattta
   112681 ttttcattct tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta
   112741 ggtgcaaatc gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat
   112801 aaagccatga gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca
   112861 tttacatcca cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt
   112921 acttttacct gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg
   112981 cttgccactg tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat
   113041 tctcctactt ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg
   113101 gcgtaatcgt ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg
   113161 ataacaatat gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata
   113221 gacgtgacga gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc
   113281 atagtctttt tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct
   113341 aagatacgcc aaggaaactg gagcaagctt atcggtgttt tttgaaacaa aaaccatgga
   113401 aataaattag ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt
   113461 ttctccattt ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc
   113521 atagtgtatg ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt
   113581 gcttctagct gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc
   113641 aacataggat accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa
   113701 ttcacgacca caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata
   113761 atcatacaag caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt
   113821 agacttatac tgacagataa agcgatgact tttttttctc tattgaatat caatccataa
   113881 gcataatata aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga
   113941 taatagatat caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca
   114001 gcatctatct ttttaaaaaa cttttctcca taataaaaac
//
LOCUS       DAFELO010000002       106869 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFELO010000002
VERSION     DAFELO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 106869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.002"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(834..3983)
                     /locus_tag="LP452_000101"
     CDS             complement(834..3983)
                     /locus_tag="LP452_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL2012866.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3997..5241)
                     /locus_tag="LP452_000102"
     CDS             complement(3997..5241)
                     /locus_tag="LP452_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL2012867.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5234..6826)
                     /locus_tag="LP452_000103"
     CDS             complement(5234..6826)
                     /locus_tag="LP452_000103"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL2012868.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7335..8060)
                     /locus_tag="LP452_000104"
     CDS             complement(7335..8060)
                     /locus_tag="LP452_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012869.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8214..8540)
                     /locus_tag="LP452_000105"
     CDS             complement(8214..8540)
                     /locus_tag="LP452_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012870.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8577..8942)
                     /locus_tag="LP452_000106"
     CDS             complement(8577..8942)
                     /locus_tag="LP452_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL2012871.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8979..9194)
                     /locus_tag="LP452_000107"
     CDS             complement(8979..9194)
                     /locus_tag="LP452_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2012872.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9323..9658
                     /locus_tag="LP452_000108"
     CDS             9323..9658
                     /locus_tag="LP452_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2012873.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9734..11731)
                     /locus_tag="LP452_000109"
     CDS             complement(9734..11731)
                     /locus_tag="LP452_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012874.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11829..12104)
                     /locus_tag="LP452_000110"
     CDS             complement(11829..12104)
                     /locus_tag="LP452_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2012875.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12336..13070
                     /locus_tag="LP452_000111"
     CDS             12336..13070
                     /locus_tag="LP452_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2012876.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            13109..14251
                     /locus_tag="LP452_000112"
     CDS             13109..14251
                     /locus_tag="LP452_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2012877.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14346..15737)
                     /gene="sufB"
                     /locus_tag="LP452_000113"
     CDS             complement(14346..15737)
                     /gene="sufB"
                     /locus_tag="LP452_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL2012878.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15742..16212)
                     /locus_tag="LP452_000114"
     CDS             complement(15742..16212)
                     /locus_tag="LP452_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL2012879.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16199..17434)
                     /locus_tag="LP452_000115"
     CDS             complement(16199..17434)
                     /locus_tag="LP452_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2012880.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17431..18717)
                     /gene="sufD"
                     /locus_tag="LP452_000116"
     CDS             complement(17431..18717)
                     /gene="sufD"
                     /locus_tag="LP452_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL2012881.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18730..19503)
                     /gene="sufC"
                     /locus_tag="LP452_000117"
     CDS             complement(18730..19503)
                     /gene="sufC"
                     /locus_tag="LP452_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL2012882.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19700..20548)
                     /locus_tag="LP452_000118"
     CDS             complement(19700..20548)
                     /locus_tag="LP452_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL2012883.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20572..21258)
                     /locus_tag="LP452_000119"
     CDS             complement(20572..21258)
                     /locus_tag="LP452_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2012884.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21251..22285)
                     /locus_tag="LP452_000120"
     CDS             complement(21251..22285)
                     /locus_tag="LP452_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2012885.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(22348..22604)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22680..23018)
                     /locus_tag="LP452_000121"
     CDS             complement(22680..23018)
                     /locus_tag="LP452_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL2012886.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(23020..23376)
                     /locus_tag="LP452_000122"
     CDS             complement(23020..23376)
                     /locus_tag="LP452_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL2012887.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23382..24569)
                     /locus_tag="LP452_000123"
     CDS             complement(23382..24569)
                     /locus_tag="LP452_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2012888.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24760..25605)
                     /locus_tag="LP452_000124"
     CDS             complement(24760..25605)
                     /locus_tag="LP452_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2012889.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25839..26804
                     /locus_tag="LP452_000125"
     CDS             25839..26804
                     /locus_tag="LP452_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL2012890.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            27181..28224
                     /locus_tag="LP452_000126"
     CDS             27181..28224
                     /locus_tag="LP452_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL2012891.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(28286..28750)
                     /locus_tag="LP452_000127"
     CDS             complement(28286..28750)
                     /locus_tag="LP452_000127"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL2012892.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28996..29943
                     /locus_tag="LP452_000128"
     CDS             28996..29943
                     /locus_tag="LP452_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2012893.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(30154..32226)
                     /locus_tag="LP452_000129"
     CDS             complement(30154..32226)
                     /locus_tag="LP452_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2012894.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(32159..32536)
                     /locus_tag="LP452_000130"
     CDS             complement(32159..32536)
                     /locus_tag="LP452_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL2012895.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(32665..33243)
                     /locus_tag="LP452_000131"
     CDS             complement(32665..33243)
                     /locus_tag="LP452_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2012896.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            33375..34667
                     /locus_tag="LP452_000132"
     CDS             33375..34667
                     /locus_tag="LP452_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2012897.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(34727..35845)
                     /locus_tag="LP452_000133"
     CDS             complement(34727..35845)
                     /locus_tag="LP452_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL2012898.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35859..37280)
                     /gene="arcD"
                     /locus_tag="LP452_000134"
     CDS             complement(35859..37280)
                     /gene="arcD"
                     /locus_tag="LP452_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL2012899.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(37526..38647)
                     /gene="mnmA"
                     /locus_tag="LP452_000135"
     CDS             complement(37526..38647)
                     /gene="mnmA"
                     /locus_tag="LP452_000135"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL2012900.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            39051..39650
                     /locus_tag="LP452_000136"
     CDS             39051..39650
                     /locus_tag="LP452_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2012901.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39788..41074)
                     /gene="efmA"
                     /locus_tag="LP452_000137"
     CDS             complement(39788..41074)
                     /gene="efmA"
                     /locus_tag="LP452_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL2012902.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(41345..41689)
                     /locus_tag="LP452_000138"
     CDS             complement(41345..41689)
                     /locus_tag="LP452_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL2012903.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41826..42971)
                     /locus_tag="LP452_000139"
     CDS             complement(41826..42971)
                     /locus_tag="LP452_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2012904.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(43151..44125)
                     /locus_tag="LP452_000140"
     CDS             complement(43151..44125)
                     /locus_tag="LP452_000140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL2012905.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(44205..45080)
                     /locus_tag="LP452_000141"
     CDS             complement(44205..45080)
                     /locus_tag="LP452_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL2012906.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            45210..47117
                     /locus_tag="LP452_000142"
     CDS             45210..47117
                     /locus_tag="LP452_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL2012907.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(47658..50936)
                     /locus_tag="LP452_000143"
     CDS             complement(47658..50936)
                     /locus_tag="LP452_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL2012908.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(51207..51752)
                     /locus_tag="LP452_000144"
     CDS             complement(51207..51752)
                     /locus_tag="LP452_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2012909.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(51764..52456)
                     /locus_tag="LP452_000145"
     CDS             complement(51764..52456)
                     /locus_tag="LP452_000145"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL2012910.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(52475..52759)
                     /locus_tag="LP452_000146"
     CDS             complement(52475..52759)
                     /locus_tag="LP452_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012911.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(52772..53335)
                     /locus_tag="LP452_000147"
     CDS             complement(52772..53335)
                     /locus_tag="LP452_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2012912.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            53563..54225
                     /locus_tag="LP452_000148"
     CDS             53563..54225
                     /locus_tag="LP452_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL2012913.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            54349..55545
                     /locus_tag="LP452_000149"
     CDS             54349..55545
                     /locus_tag="LP452_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL2012914.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(55646..57022)
                     /locus_tag="LP452_000150"
     CDS             complement(55646..57022)
                     /locus_tag="LP452_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2012915.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(57059..57925)
                     /locus_tag="LP452_000151"
     CDS             complement(57059..57925)
                     /locus_tag="LP452_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL2012916.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(58014..59039)
                     /locus_tag="LP452_000152"
     CDS             complement(58014..59039)
                     /locus_tag="LP452_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2012917.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(59057..59731)
                     /locus_tag="LP452_000153"
     CDS             complement(59057..59731)
                     /locus_tag="LP452_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2012918.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(59915..60808)
                     /locus_tag="LP452_000154"
     CDS             complement(59915..60808)
                     /locus_tag="LP452_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2012919.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(60882..62579)
                     /locus_tag="LP452_000155"
     CDS             complement(60882..62579)
                     /locus_tag="LP452_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2012920.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(62572..63885)
                     /locus_tag="LP452_000156"
     CDS             complement(62572..63885)
                     /locus_tag="LP452_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2012921.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(63885..67403)
                     /locus_tag="LP452_000157"
     CDS             complement(63885..67403)
                     /locus_tag="LP452_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL2012922.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(67696..68154)
                     /locus_tag="LP452_000158"
     CDS             complement(67696..68154)
                     /locus_tag="LP452_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2012923.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(68452..68970)
                     /locus_tag="LP452_000159"
     CDS             complement(68452..68970)
                     /locus_tag="LP452_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL2012924.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(68972..69589)
                     /locus_tag="LP452_000160"
     CDS             complement(68972..69589)
                     /locus_tag="LP452_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL2012925.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(69586..70440)
                     /locus_tag="LP452_000161"
     CDS             complement(69586..70440)
                     /locus_tag="LP452_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2012926.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(70437..71348)
                     /locus_tag="LP452_000162"
     CDS             complement(70437..71348)
                     /locus_tag="LP452_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2012927.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(71389..72723)
                     /locus_tag="LP452_000163"
     CDS             complement(71389..72723)
                     /locus_tag="LP452_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2012928.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            72931..73761
                     /locus_tag="LP452_000164"
     CDS             72931..73761
                     /locus_tag="LP452_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL2012929.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(74005..74323)
                     /locus_tag="LP452_000165"
                     /pseudo
     CDS             complement(74005..74323)
                     /locus_tag="LP452_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(74445..74639)
                     /locus_tag="LP452_000166"
     CDS             complement(74445..74639)
                     /locus_tag="LP452_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012930.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(75144..75329)
                     /locus_tag="LP452_000167"
     CDS             complement(75144..75329)
                     /locus_tag="LP452_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012931.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(76179..76520)
                     /locus_tag="LP452_000168"
     CDS             complement(76179..76520)
                     /locus_tag="LP452_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2012932.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(76520..76750)
                     /locus_tag="LP452_000169"
     CDS             complement(76520..76750)
                     /locus_tag="LP452_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2012933.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            77037..77375
                     /locus_tag="LP452_000170"
     CDS             77037..77375
                     /locus_tag="LP452_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012934.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(77580..77819)
                     /locus_tag="LP452_000171"
     CDS             complement(77580..77819)
                     /locus_tag="LP452_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL2012935.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(77795..78142)
                     /locus_tag="LP452_000172"
     CDS             complement(77795..78142)
                     /locus_tag="LP452_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL2012936.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(78874..79455)
                     /locus_tag="LP452_000173"
     CDS             complement(78874..79455)
                     /locus_tag="LP452_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2012937.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
     gene            complement(79638..80264)
                     /locus_tag="LP452_000174"
     CDS             complement(79638..80264)
                     /locus_tag="LP452_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2012938.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(80286..81617)
                     /locus_tag="LP452_000175"
     CDS             complement(80286..81617)
                     /locus_tag="LP452_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL2012939.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(81772..82173)
                     /locus_tag="LP452_000176"
     CDS             complement(81772..82173)
                     /locus_tag="LP452_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL2012940.1"
                     /translation="MKKAYETAMINHGGRNGEVEEPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVFEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(82259..82465)
                     /locus_tag="LP452_000177"
     CDS             complement(82259..82465)
                     /locus_tag="LP452_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012941.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(82560..84062)
                     /locus_tag="LP452_000178"
     CDS             complement(82560..84062)
                     /locus_tag="LP452_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL2012942.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(84080..84535)
                     /gene="argR"
                     /locus_tag="LP452_000179"
     CDS             complement(84080..84535)
                     /gene="argR"
                     /locus_tag="LP452_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL2012943.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(84633..85250)
                     /locus_tag="LP452_000180"
     CDS             complement(84633..85250)
                     /locus_tag="LP452_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL2012944.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            85471..86844
                     /locus_tag="LP452_000181"
     CDS             85471..86844
                     /locus_tag="LP452_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2012945.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            86919..88031
                     /locus_tag="LP452_000182"
     CDS             86919..88031
                     /locus_tag="LP452_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL2012946.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(88103..89740)
                     /locus_tag="LP452_000183"
     CDS             complement(88103..89740)
                     /locus_tag="LP452_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL2012947.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(89908..90516)
                     /locus_tag="LP452_000184"
     CDS             complement(89908..90516)
                     /locus_tag="LP452_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2012948.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(90901..92052)
                     /gene="queG"
                     /locus_tag="LP452_000185"
     CDS             complement(90901..92052)
                     /gene="queG"
                     /locus_tag="LP452_000185"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL2012949.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(92071..92823)
                     /locus_tag="LP452_000186"
     CDS             complement(92071..92823)
                     /locus_tag="LP452_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012950.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(92964..93914)
                     /locus_tag="LP452_000187"
     CDS             complement(92964..93914)
                     /locus_tag="LP452_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL2012951.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(93915..94754)
                     /locus_tag="LP452_000188"
     CDS             complement(93915..94754)
                     /locus_tag="LP452_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2012952.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(94751..95497)
                     /locus_tag="LP452_000189"
     CDS             complement(94751..95497)
                     /locus_tag="LP452_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2012953.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            95735..97105
                     /locus_tag="LP452_000190"
     CDS             95735..97105
                     /locus_tag="LP452_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2012954.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(97160..98056)
                     /locus_tag="LP452_000191"
     CDS             complement(97160..98056)
                     /locus_tag="LP452_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2012955.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(98170..98340)
                     /locus_tag="LP452_000192"
     CDS             complement(98170..98340)
                     /locus_tag="LP452_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012956.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(98337..99014)
                     /locus_tag="LP452_000193"
     CDS             complement(98337..99014)
                     /locus_tag="LP452_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2012957.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(99105..100010)
                     /locus_tag="LP452_000194"
     CDS             complement(99105..100010)
                     /locus_tag="LP452_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2012958.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            100185..101639
                     /locus_tag="LP452_000195"
     CDS             100185..101639
                     /locus_tag="LP452_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2012959.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            101655..102686
                     /locus_tag="LP452_000196"
     CDS             101655..102686
                     /locus_tag="LP452_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2012960.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            102693..103880
                     /locus_tag="LP452_000197"
     CDS             102693..103880
                     /locus_tag="LP452_000197"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL2012961.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            104036..105418
                     /locus_tag="LP452_000198"
     CDS             104036..105418
                     /locus_tag="LP452_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2012962.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            105390..105776
                     /locus_tag="LP452_000199"
     CDS             105390..105776
                     /locus_tag="LP452_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012963.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(105976..106644)
                     /locus_tag="LP452_000200"
     CDS             complement(105976..106644)
                     /locus_tag="LP452_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL2012964.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        30615 a        22286 c        18827 g        35141 t
ORIGIN      
        1 ggctatggaa aggcaaggag cctttatttt tttgttcaaa aattcgaaac gaagtagata
       61 aaaagctgtt ccaagctcga gttacgagtt tggaacagct tttgtctaca gtctgaggac
      121 taatacaggt agtcctcttt tttgttattt tgatattcta aatcattttt tattggatta
      181 ttcccttgtt cgatatagac ccaaattgat ctatcaataa ttatcaaact gagtcatata
      241 gagacaaaaa tttcaaaaat gacctgttaa atggtcatcg aatatcattt aaaatcaata
      301 cgacggagtc agacacggag tcaaaacaag cccgctgaga gtaaaaaata tatacagtcc
      361 cataggtaaa gtgccacagg actgtatatt tttttatttg agattacctg aaatgattcc
      421 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
      481 ataaactatt ttgggttaca tggtatctga aacagctaaa accttgtcat tacagcaaaa
      541 aatcacttta tctccgtttc atttcagata catttcactt aagcggattc caaaaaaaca
      601 aaagaaaaag gaacccctag ttagcctatg ctttttattg gttcctctca aaacacggtc
      661 atataacaac ggggtggggt aaatgaatga aaaaggtgga ggaaacatta ttcaatagct
      721 gtcttaaata cttcgttata aaatgtgaac aaataaaaga catctatccc tattttctac
      781 acttccactt tactaccatc tttgaatcgt acaattttct taactcttct cgtttacctc
      841 aattcatttt caagtggaat cagctcatca tctaacattt tacgccatgc tcgcttcatt
      901 tcttgtgggt atttaaatgg cttcgcttct ggatggttgg ctttatatcc ttcgtaatcc
      961 ttactgttga taattgcttt catattagga attggatctt cacctggcat atattgcaga
     1021 ttagaagcat gtaattcttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa
     1081 tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attactatct
     1141 aaagtcccca catcaaacgc atttaaaatt tgtcggtaaa cttctttgac tccttctgtt
     1201 ttattcaatt tagccaaagc tttttcaatt tcttctcgtg catctgaact attcgccgca
     1261 taagttccta gtaattgatc aatataagta gaatcaatat catacaactt cgtggtattt
     1321 tcactataaa attcgatcgt ggaaaaatct tctccctctt tttctggttc ttcctctatt
     1381 aatgaaccct tcacggtctc gtacacgcca atttgttctt ccaaaagttg aatttgattg
     1441 ttcaaggttg aagatttcac catgtcatca ttgtaatcgt cataagtcac taacgattca
     1501 taagcacgat tcaattcttg gaatgctttg acgtactcga tacggatttc aagtggcgta
     1561 tgttcatcaa tggattccat ggatgaaacc tcttttaact taccatatgc ttgcttgaat
     1621 ctttttttgg attcctgata acttggataa atcaaacccg attcttcttt tgcttctgaa
     1681 tacagttttg tcgcatcctc gacattcttc tccattgtgg ctggctttcg gaaagtaaca
     1741 attagtcctt ttgttttttc tggatacgta cggttcgtac gtgaaaaagc ttggatcaat
     1801 cctgcatact ctagattacg atccacaaat agggtttgaa tcgtaggtgc gtcaaaacct
     1861 gtcaatagac gatccactac gatcactaaa tcaatttgtt tcccaaattg tttgaattcc
     1921 gctctttttc gtgccaaacg attattgatg tctccgttat aacgctcaat gtctgctaat
     1981 gaccaggaag tattataata ctgattatat tcctcaataa ttttttgcat ttcttcttgt
     2041 tggacacttg aatctcggct gttttcttct aatgaatagg taatagcgat tcgtggaaaa
     2101 tcaggatcct ccatcgttct tccttcacga atgggctgat taggaaactc ttttgttaac
     2161 cagtcaggtt ctttcgtcat ttctttgatc gctcgataat actttttggc catgtcaatg
     2221 gaactcgtgg ttaagatcgc agacttcgtt ggacggccat ttcgaaaatc aaattttgta
     2281 taggcattgt ctggacggaa cattttatga atgacttttt ggatatgttc atctgcttca
     2341 aaaacagtag ggtccaaata actttctttt ttgacaggct ccatttgatc aatcatacga
     2401 ttgatctgtt ccggtgaata ctctgcatat gtttctactt cttttaattt ccgatagatt
     2461 ttattctcta atgaagtcgg ctccacggtg ttttcatgct ctacttgaaa tcctaaaacc
     2521 gagccatctt ctaatgcatt cttgatggta taggtatgca agacctctcc atactgatca
     2581 tgggtcgtac gtgccaattg ccctttggct tgtttccgat tctcagggaa aatcggtgta
     2641 cctgtaaagc caaaccaagt ggatttcgga aacatttttt tgatttcttc catattttca
     2701 gcacttaacg ctcgatgaca ctcatctact acaaacacga tatgttgccc cattaatttt
     2761 tggaagcgat tggttccttt tttctcttct tgtttttttg catatttcaa cgccgcatct
     2821 agcttttgac gggttgtaat aatcacaaca tttgcattgg catctgccaa cagagtttca
     2881 cttaactctt tggcacttcc tgttcccacg atcaagctat tcgctttggc gttcccggaa
     2941 gaaatcccag tattaaattc cgaagcaaat ttcgtaaact ctgtggtggt ttgattatct
     3001 aaatctttgc ggtccactaa catgatcgtc cgatcgatcc ccgattttcg tgctaaaagc
     3061 ttagtagata caaaactagt caacgttttt cctgaaccag tcgcatgcca cacatatcct
     3121 gattgatgtt tatttgcagc aataaacaag gcttgaatcg catgtacttg ataaggatgt
     3181 aacaccatga ggattttatt gtcttgatct tcactgacaa tcgtataatc cgcaatcaag
     3241 cgatgagcat ccgggatatt tagcacttgt ttgcaaaact catataagtt ttctaccttt
     3301 ttattatctt tcgttcgcca actaaataaa aacttaggat gcaaatcctt tggtaaggca
     3361 tttgcaaaat accgagtggt ttgttcattg gaaaccacaa acaactgtaa ggtcgaaaag
     3421 atattgttcc gaaacatacc ttcttctgca tattttttga tttggttaaa cgcctgataa
     3481 aaactatctt ttgcggttac ttgtttcagc tcgatttgga caatcggtaa tccattgatc
     3541 agtaaggtga cgtcaaaacg tcgatcccgt gcttctatgt tacttccacg ttttgcaatt
     3601 tgatggacca cttcataggt agagattcct ccaccaatgt cttgattaga ataaagaatg
     3661 agcgagacag aacccagttg cggatcttcc cgttcaatcg tgatccgagc cattccattt
     3721 tcacctttta accatttcgc agcatcaaag ggggttttcg ttcgtagtaa caattctgtt
     3781 ttgatcgttt caaattcttt atcggtcaaa ggaaaatcat tcagttctgc ttgattattt
     3841 gagatgattt tttggcgtaa attgacccaa agatcttctt ctgattttag atcgggacga
     3901 tacgtccact gattatgccc ttcccccaat acacggatca actgttcttc gacttccgct
     3961 tcctcacggt gactaatttt catttatctt ccctccctaa acaaacatct tttgtaagaa
     4021 gccttttttc gtttccttca gcaaatctaa cttacgttga tgaagagcga tcgtgtcgtc
     4081 gagttgtttg aagaaagcgc cgattttttg ttgttcctct ttattcctag taaaaggaaa
     4141 cttgatatta gcgaaattct catagaataa gtattctcgt acgcttcctt cagtgttgcg
     4201 cttgacctct ttgataaatg ttggtgactt gataaactga agtatgaact cattatctaa
     4261 gtcctcgctc attttcacaa ctacataaag agagctaaca atgaccgtca taccaagatt
     4321 gttaaatgca attgatccta cgtttactct tgctggatta taggcgaatt catttggttc
     4381 aacgaattta taatttgttt tttctaaatc atccaaccta ctaccatcaa actgttctgt
     4441 ttgagaaatt aaacctgctt tattacttac cgaataggca gggaagtcta aatctttcgt
     4501 gttctttttt ccagtcttct tactaaactc tttgaactta cgctgttccc aatcttccgt
     4561 aaatccagga aaccgaattt ctggcacttt tgctccgttt ttagggaaca ttttttgtaa
     4621 gaatcctttt ttcgtttcct ttaataaatc tagcttacgt tgttgaagag cgatagtgtc
     4681 gtccagttgt ttgaagaagt tcccaatttt cttttgttct tctaatattg gaaattgtac
     4741 tatccatttt ttcacttctg acattggtac gagatttgta attgctgaac ctggattagc
     4801 tcctgtgagt tttctttgca taaaatctgc acgaataaat tgaataaaat attctgaatc
     4861 tgtgatttct ttatttattc taagtctaat caaagcctga ttgaatacac ccggttttat
     4921 ttgctttggt actcttgaaa ttctcccaat tgttccagcg ccactcatga taaaatcgcc
     4981 tggttctaat ttaaatttga ttaattcatt atattttttt tcagtaattt tatatcttgt
     5041 ttcgtaatta tcataaatag cattttgttg ttcatagaca acaaaattgc tattagatac
     5101 aaagaattct ttctttaagg cactcccaaa tggtcccctc cttactccat cttttttatc
     5161 aagtaagcat tcaaacttac gctgttccca agcttccgta aatcctggga aacgaatttc
     5221 tggttgtata tcattactca tgtttgaaca cctccagcgc accttgtaac cacgcttcat
     5281 tttcaggtgt tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct
     5341 gttcttgtcg tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt
     5401 cttcaaacgt atctacatag cgtggaatat ttaagttata gtcattttct ttgatttcat
     5461 caaagtttgc caaatgagcg tatttctcga catcttttct ttcagcatag gtttcgagga
     5521 ttttttgaat gttttcttcc gataatttat tctgattttt tcctttgaca aattctcgac
     5581 tagcgtcaat gaataatacg tctcgagttt ggcggttctt tttcaataca atcaccgtcg
     5641 ttggaatcga tgtcccgaag aacaagttcg caggcatacc gatcacagca taaatactgc
     5701 cgtcttctag taatttttga cgaatgaccc cttctgcagc gccacggaat aaaactccgt
     5761 gtggcaagac gatggccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa
     5821 acgcaaaatc tgctttggat tttggcgcta attttccata acgattaaac cgagaatcgt
     5881 ctaagaaggt cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat
     5941 caaatgtgta aggttcatct gttggccaat ccttattcaa cgtatcccca ttgcggagat
     6001 tcatttcttc ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg
     6061 ttgtatttaa ttcttgccca tgatatttaa cgttatctgg gtgagttaag tagttacgca
     6121 catttaacat caatgaacct gaacccatgg ttggatcaaa aacactaaag aatcgtcgtt
     6181 ctttttgatc caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa
     6241 attctccggc tttcttccct gcttctgaag caaattgact gatcagaaat tcataagcat
     6301 ccccgatcac gtccccatcg tgacctaaaa cttccacgtc attcaatttc ttaatgactt
     6361 cggtaatcgt cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca
     6421 catcatcaaa taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt
     6481 ttagatcatt caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat
     6541 actctggcga aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg
     6601 attcttcatc tgaaagtaat tcgcgataca acatcgtttg tttgcttacc gtatcatatt
     6661 cctctaagga ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt
     6721 tatagaaaat cagtcccaat aaataatttt tatattctga cgcatccatt ttgctacgga
     6781 gattatctgc cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct
     6841 atctcttatt taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag
     6901 caaaaaaaaa agaaagaaac ttattcttgc taataaacta cgcgaaagga gtctaacgtt
     6961 gtttgatata acttagactg atcgttaact tattttttat atttctacat agttatcttt
     7021 agaaccattt cagatacata tgaaacaatt acatatgatt ttttcataaa aaaacagtac
     7081 tgtctagtag tataaaatat acaaatttct taaaaatttt ttcactaaaa tcactacctg
     7141 aaacagtcta aaacacttat atatcaacgt ttaatgaaac tatctccgtt tcatttcaga
     7201 tacaaataaa tatagtcggg ctccaaatcc gaaaaaaaat acccccagta gcttatccta
     7261 agctactgga gatgtataag tacggtcata taacaacggg gtagggtaaa taaatgaaaa
     7321 agatggaaga atcattattc aataactttt ttaaatactt cgttgtaatc agttacctgt
     7381 ccgttcttaa ataccacaga ataatctttt ttcccatttt caagtaaccg aagtggaata
     7441 tctttaccaa ctttctctgt cactgcatta ctgatacctg ttagattagc taataaatct
     7501 gataaagatt gagcatctgt agaaatctct gtatcagtat ttatcgtgat tcctttatct
     7561 tcatttacaa gtttccaagt aggtggtata gctgtgtatg tttctacaaa tgaattaagt
     7621 ttttcattaa gtgacttctc ttttatattt ccatctaaaa actctgtata tatggcttga
     7681 gctatactaa aacaaagctg gccttgttct aataatttta catcattagt tagtaaagct
     7741 gtgttcacgt tatcaatcgc ttcaagaaga gtatctttgg tgtccctaac agctttaggt
     7801 gtgccttctg gaatttccct acttgatacc tcgtccattt tatcttccat aaacccctta
     7861 aaatcatatc tttctcccaa atccatagaa gtttttgaga aagctttcat ttctgagtct
     7921 atttcttcaa taatatttaa catgtctgat acatattgtt taggctgatt cgtttttaat
     7981 ctatatgtgg taaatatctc aattcctcca attatcaata aaattgccaa acaaatccat
     8041 aataattttt tctttttcat ttttcctcct atacactgct gttataacag tgactaatgt
     8101 aaagattttc ccagcaatca tcagactatt atttacaggg aatcaaaaaa actccttagt
     8161 aagcttacac ctttaggagt tcctgtttga acggtatgca taatacattt aaatcaattt
     8221 aacattaaca cctcagtata tggtatctct acatatacac ggtctttttt tggatagtac
     8281 tcgacattta tattgatagc acttccattg tctaacgtag ctttagcccg gtacgtgtta
     8341 tcaccattat catgagtgat cgtcacatca tcacatgtcc gatttaagtt atactgttct
     8401 tgtaaaatct gagttaccaa tggtacagat gacttctcta gtccagatga acccgtcatt
     8461 cttgaaaaaa ttatacctat aatagccaat acaacgatac ctataatgac aaacttctta
     8521 ttcatattta ctttattcaa ctttttatct cctatctcca aaatgatttt ctctcatcag
     8581 taactcattg acatccccaa tagtacacca aagatggctc cagctacccc tgctatcatc
     8641 aaaatcgtac ccatttgttc atctttctct cgatataagt aacccataat aacagcaaca
     8701 gcaccaaata ccacaggaat aaatgctaat gatactacca tagaaatcca accaataatc
     8761 aaatacagct tccctgtctc ttgtccatgt acgatttgtt ccttcatttc attaattgtc
     8821 tgctcagtac ttttctttac tgtgtctgtt ccggttgtct catctgtttc ttgtaatgct
     8881 ccgcaaaact ggcaatactt actttcattt gttatttctt taccacattt tctacaatac
     8941 atgtcttctc ctcctaaaat cattcaattg cttattagct aactgtttac tttattaaat
     9001 acatcagaaa acagctcatt tattggtaca cttaatatct cagaaagcat tttcatttga
     9061 tagggtttag gaatcgttct ccctgtttgc cacgaactta cacattgctg tgtaacattc
     9121 atttttttgg ctaactcagt ttgggaaagt cctttttctt ttctgtatcg gctgagatta
     9181 cttacttttt tcatgattct cccctcctgt tttattatat acaacaatat gatgtatttc
     9241 aactcataaa atatataaat acttcaaatg tcattacaaa tacaacaact tgttgtactc
     9301 tactgagaag gggggtgtat taatgaaaca gaagcaatat gcaaaggtca ttagagaaaa
     9361 gcgaaaagca ctcgggtgga cacaaaaaga attagctaaa aaaatcttct ctacacaaca
     9421 ggcagtagca agatgggaaa attctgtaac agaacctaac ctcgatagct taacagctct
     9481 ttctagagcc ctaggaaccc ctgtaagcca ttttctagac aatgttgaca tagattatga
     9541 ggaagagttt ttggctctct atcgctctct tagtactgaa gatgcaattc gaacaattga
     9601 ctatatgaag ctattaaaaa ggcaagaaaa tgaacgcaat cagctattga aagattgaaa
     9661 aataaataac ccaccgaact ttaacggtgg gtgtattgag atgaaataag attaagagct
     9721 atggataacc tgtctagttt aatataattg ggtctcttct attccttact ctccgtccat
     9781 catctcttgt tacacttgta aattgataaa taggaactaa ttcaactcct gataatatca
     9841 attcttttat tacttcatct atccctgccc ctttcgctag atatactaaa aaactactgc
     9901 ttgctttcac attgtatagt ttcttaatat ccttagaagc tcgttcaaga gtgaatcctt
     9961 gtttaacaaa gtagcctctg ttttctatta aagcattttt cacgctgtta tccctgacat
    10021 ttcgtaggcg ttcacaaaca gtgttaataa gttctttttc tgtcattaga tatgtccctt
    10081 gccacgataa gccctgtaaa acttcatgtg cttcatctaa aatcaattgt ttttccttgc
    10141 taagttctga tcgggaattt tgaggaaata ctcttttagc ctctttttcg ttaaaacata
    10201 tcttaaccca ttcataagaa aaatattgat atgtaaaatg aaatttttcc agaagtggaa
    10261 ggatatcttc attcaatttt tgaatcacag aagttgcatt aaaaacgtct aatcgataga
    10321 cattgcttct cttatgttta aagtttccta acgctttctc ttcctttaac ctatttaggt
    10381 attcgagcat cttcaaagga acatcttcat catctaattt ttttatcaat cctataaaag
    10441 tcataagtga tagtaactta ttacatttat ctatgtcata agttttacca tagtaagaac
    10501 tcattttttt agctaattgt tcccccgaca aaatacaagt cacactttcc ttactatcat
    10561 caaaaaaatt aacatcagaa agcaagacat catagacacg tcgcatttcc tctagataat
    10621 tccttattgt cctgaaaagt ttgtctttca ttgattcaag agtgtcaaaa agtacttttg
    10681 attcctcttt caattttcga aaatgtgtag gatacaagtc taggttctct aatagccaat
    10741 taaatgtttc tacccgactg tctttcttta aaattagcat cacaactcca ataaaatttt
    10801 cactgatatc acaattactg gaatgacaat aaaaaatgga atgcccagtt ttactggtaa
    10861 aaatactagc agagggatta tgatcgttgt gaaacagaca tcttaagttt ttacttgact
    10921 taagtaattt attcatatca atggttaaca atctttcata aatctcatta aaagacatgt
    10981 cggaggaatc aaacaggcag tctttaaacc actgtttcat ttcttctttg tcgtcatttt
    11041 ttatcaactt aacaaaatcg gtttcattga ctagttcata ctctctccct gaatcacttt
    11101 gacaattttt agaacatgtc atggtaaaat caatcggaag ctcagtaata tctaacatgt
    11161 tgtcttcatt aattattatc actgcatctg acttatatcc tccaaaaaac attctattcg
    11221 aagcatttac attgtcatca cacccgggga tgttcttttt caagtattcg taaacagcaa
    11281 ccattttacc atagtcagta attgcttctt ttaatagaaa gacaatcttg tatttttcta
    11341 gattttcaga gtaccgaata cttttttgta tcgcaaacgc agaattatga actaaattga
    11401 tagataaaac cttttctatg cttaagtatc tactatcatc tttactcaaa tctactacct
    11461 tccccttctc atctttaaca gtgttatcga catccagaat aattactctg gtttccacaa
    11521 aactctcctt gtcattgcct ttattctttg tcttgcttgg aataaatgat tgtgatgaat
    11581 ttagtatctc tacaaactca tctcttgtaa cttctctata gttatctaaa cacctatctc
    11641 ttacatcagt tttttctttt tttgtaactg gcttctctaa taatttttct tgatctacat
    11701 aaattggaat attaatcatt gatgtttcca tttgtgtttc ctccttataa aattctgtta
    11761 aagactaaaa catacagata ataaaaacaa actctttatt actatatgtt ttagtcttat
    11821 ttgtttactc aacctttata ccgttccatg aaactatcaa tatcagattt tttaaatcta
    11881 atgaccgtat caatttttat aacaggtaac ccgtattgag aaacatattt tctaatcgtt
    11941 cctactgata cacctagata ttcacttgcc tgctgatagt ccatatactc tagagtgact
    12001 ccagaaataa tgtcttctag cataggcttt agcactgtaa ttatctcttt cacttgttgt
    12061 tcaacttgta tagaaacaat gctatcaatg atttgtagag acatatttcc ccctcctttc
    12121 gtgattgctt gaacaaattt tctaagaatt atcttcgtat tttttgctca aaataaaagt
    12181 attttgttat taaccgatta atcaaacaaa aaatgattac cagttcatcg acatacacat
    12241 aatacctcga taaaaaaggg gtgtcaacag ctttgtaaaa tttatttaca acaccccttt
    12301 tttgaggtat actaatttta ggaggtgtgg tcaatatgat aaaacgtcca ccaataaatt
    12361 atttagaacg aaaaaagata ctcggaacaa aaataaaagc tataagaaag agtaaaaaat
    12421 taacacaacc agcatttggt cttatgatta acaacggtca attaattgac aaaaaaacta
    12481 tctacgaatg ggagaaggga acctatttac caataccaga gcgattatcc cgaattgctg
    12541 atttgggaaa tatgagtata gaggagttag tttgtggcaa tgtagaagaa tatattttgg
    12601 gaattattct ttatagagat agcattgtac tagatggtat aacatttcca gataaaaacc
    12661 tgttccagca tcttcgtcaa cagttcccac cagtacactc taatttagat acatggctgg
    12721 atagatattc caaattagaa ccagagatgc aagaatttat tgctaacaag acttgtaata
    12781 aagttaaaaa tgaaaaaatt agtcttttca atatcttaaa aatagaagaa ttattcatca
    12841 atgctattgt agaagaattt gacaataaca ttcttttcct cactagcagt atagaagaac
    12901 tactagaaag aatggtagat gaatggctac ctattcaatt gaaagacatg tcttatcccg
    12961 aagaagctgt gagagaaatt actgataata taaacaagct agaacaaacg atttcatcaa
    13021 ttgggaaaaa atacactaag aaaaaaatga agggaggtga taccatctag tggtaagtac
    13081 tccttgtaaa ctaagaggag atgatttaat ggcaaaaata tcaaagtaca aaaagaaaaa
    13141 cggtcaagtg gcgtggatgt ataaaggaca cattgccacc gatccacgaa ctggtgaaaa
    13201 aataaatact acgagaagag gttttagtag caaacaagaa gctcaaaaag attatgacga
    13261 gtacagacat caaatattat acggtgttaa aaagaaagca cctgatatga catttgaaga
    13321 cctgtataat gaatggataa ctcatcagcg taccagtgtt aaagcttcaa caatcgcaat
    13381 ctcagtaaga tacgcaaata atcaaatact tccagcattt ggtaagctta aactatcgaa
    13441 tatctctgtc ccatattgtc aaaaagtggt agatgaatgg cacagtaaat atgaaagtta
    13501 cgattatatg agaaaacaga ctgcacaaat tttacgctac ggggttgcaa tgcaatatat
    13561 tgacaataat cctatggaaa aaacacttct tccacgaaaa aaagagtatg agaaaaatcg
    13621 taaattttac tcaaaagagg agcttaataa tttactggat gcttttaaag attttggtaa
    13681 catgaaacaa tacgccttct tcagattatt ggcatacaca ggtatgagaa agagtgaagt
    13741 actcgcttta cagtggaaag acatagacac gtttaataaa gaactacatg tcaataaaac
    13801 tcttgctgta gatgaatttg gtaaagttat aattcaatct ccgaaaacaa gagcttctag
    13861 acgagttatc tctttagata ctgagactct ttctatcctc aacaactgga aattacagca
    13921 aaaagaagag tatttaaaac ttggatacaa tactagctct aaagagcaac atgtttttac
    13981 aacagttaaa aacactctat acattccaaa cactgttaat gattggttaa gatatatttt
    14041 gaaaaaatat aacttgcctc gtattacacc tcatgggttt agacatacac atgctagttt
    14101 attattagaa gcaggagaat ctgtcaaggt agttcaacaa cgattagggc atgaaaatag
    14161 taaagtgaca ttagacatct atgcacatat tacaaataat gcacctaaga aaacaggtca
    14221 agactttgct gatatgatgg ctcatcagta aaagagctgg tcaaaatctg gtcatttcta
    14281 gtatacaggc aaaacaaaaa agctagaacc cttatatatc aagggttcta gctcctataa
    14341 caggtttacc caacactgcc ttccatctca taactgatca atcgattgag ttcgactgca
    14401 tattccattg gaagttcttt ggtgaatggt tcaacaaagc ccatgacgat catttcagtt
    14461 gcttcagatt cggtcaggcc tcggctcatc agatagtaga gttgttcttc tgagattttt
    14521 gagactttgg cttcgtgttc taaagagact tggctgttgt ggatctcatt gaatgggatc
    14581 gtatcggatt tcgaccattc gtccatgatg atcgtgtcac attcgatatg agagatcgaa
    14641 cccgcactgt ttttggcaaa ggttacttgt cctcgatagt ttacttctcc gccgtctttt
    14701 gaaattgatt tagagacgat agagctagat gtatttggtg cattgtggat catttttgca
    14761 cccgtatctt ggatttggtt tgctcctgca aaggcgatgg agagcatggt gcctcgtgca
    14821 ccttttccat ctaagtaaac acttgggtat ttcatcgttg tttttgcacc taggtttccg
    14881 tcgatccatt caacggttgc gccttcgtat gcttttgctc gtttggttac taagttgtac
    14941 acattgtctg accagttttg gatcgttgtg taacggcaat acgcatcttt tcttgtaaag
    15001 atttctacga tggcggcatg caggctgtta cttgaataag taggtgccgt acaaccttcg
    15061 acatagtgga tgcttgctcc ttcgtcaaca atgatcagcg tacgttcgaa ttgccccgtg
    15121 ttttccgcat tgatccggaa gtacgtttgt aatggaacat cgactttgac gccttttggc
    15181 acgtaaataa aggtcccacc tgaccatacg gctgaattca gcgctgctaa tttgttgtcg
    15241 gttggcggta caagttttga aaagtactct ttgaataatt cagggtattc tttcaaagca
    15301 gagtcagtgt cagtaaagac gattcctaac ttttcgaatt catctttcat attatggtac
    15361 acgacttctg attcatattg tgcggaagca ccggctagat aagctcgttc agcttctggt
    15421 ataccaatct tttcaaatgt atctttgatt ttttctggta cgtcttccca gtcacgagcg
    15481 ggtttgtcac ttggtttttg gtaatatttg atggcatcga aatcgatgtc tgaaaggtcg
    15541 gggccccatg tttgcattgg cattttgtta aatgtttcta gagatttcag acggaattca
    15601 agcatccatt ctggttcgcc tttgattctt gacatctcac ggatcacttc ttctgtcaag
    15661 ccttctcctg tactgaaaac cggttcgaca tcatcgtgaa acccgaattt atattcttct
    15721 aattctggta cgcccatgtt ctcactcctc ttccttgtgg taatgatgga aagctgcggt
    15781 accatcgttt tccattgctt ttcctagtgc tttccaggca agtgtcgcac atttgatccg
    15841 agcgggaaat ttagctacac caccaagcat agcagcatcg cctaattggt cgggatcttt
    15901 gacttctttt ccttggacaa gctgtagaaa gtctacagtt aaatgttctg cttcttctat
    15961 tgattttccg atgacggcgt ctgtcatcat cgaggcactt gccgtactga tggagcagcc
    16021 actgccgtca aaggcgatat ttttgatgat tccgtctttt acatcgactt gcaattcgat
    16081 cacatctcca catgtggggt tgttcaattc gatcttgctt gtcgagtcag tcaagcttcc
    16141 acgatgatgc ggatgggaag aatgatccaa gatcacttga cggtataaat tgtctaattt
    16201 agatagtccc atgttggaaa aactcctttg ttgccatgat tgcttcaact aaacggtcgg
    16261 cgtcttgcgt tgtgttgtag aaataaaagc tcgcgcgagc agtcgctgat acttcaaggt
    16321 acttcaatag cggttgcgca caatgatgac ctgctctgac ggctacccct tccatgtcta
    16381 aagctgtcgc gacatcatgg ggatgtaacc catccaaatt gaatgcaacg actcctgtcc
    16441 gaaaattcgg gtcttgtgga ccataaacag tcaatccatc aattgcttgt aattttggca
    16501 acacataagc ggctagttct tcttcatatc gatggatatt gtccataccg agtttttgca
    16561 aataatcgat tgcatggcct aatgcgatag cgccggcgat atttggtgtc cctgcttcga
    16621 atttccaagg tagttctttc catgtactgt cgtataattc gacaaaatcg atcatttcgc
    16681 caccaaattc aacaggttcc atttcttcta accattttcg tttaccatat aaaacaccaa
    16741 tacctgttgg tccgcacatt ttgtgtccgc tgaaagcaaa gaaatcacag tctagtgctt
    16801 ggacatcaat ggccatgtgc ggggcagatt gagcaccatc tactacgatg accccgccgt
    16861 tttcatggac aagttcagct aattcttgaa caggattgaa gacacctaag acattggata
    16921 catgagcaat ggagacgatt ttggttttgt ccgtaatctt tttcgctgca tcctccatat
    16981 ccaggaagcc ttcttctgtt agttcgatat atttcaagac agcacctgtt cgttttgcca
    17041 actgctgcca cgggataaca ttagaatgat gttccatgta agagagcact atctcgtctc
    17101 ctgctttgat atagcggtcg ccgaaggttc tggctaccca attcaagctt gtcgtcgtac
    17161 cacgagtaaa cagcacttct gctgtttctt ttgcatggat gaacgcccgt actttttctc
    17221 ttgcttgttc ataatcatgg gtcgcgcgtt ctgctaacgt atggacaccg cgatgaacat
    17281 tggcgttatc gtgttcgtaa tagtacgcta atgcctggag tacttgtttt ggcttttggg
    17341 tcgtcgctgc attatccaga taaactaaag gttcatcatt gacgtcttga aataagatag
    17401 gaaaatcatt gcgtagcttt ttagcatcga tcatccattc aacttccctt cgattacttc
    17461 gactaattct ttccgaactt gttctacagg gattgctgtt aagacagaac cgaggaatcc
    17521 tcgaatcact aagcgctctg cttcttcttt atgcaatcct ctactcatca gatagtacat
    17581 ttcttctgga tcaactcgtc ctacactggc agcatgtcca gcagttactt cgttttcatc
    17641 gatcaaaaga ataggattcg catcaccacg tgctttatca gaaagcatca agacacgact
    17701 ttcttgttgg gcatctgcac cttttgcccc tttcaagatg tggccgatac cattaaaggt
    17761 caatgttcct ttttctcgga tcactccgtg ttgcaaaata tgcccgatcg tatgaggggc
    17821 tttattggtt actcgtgtat cgattccttg gatttgtctg cccgagctga ttgccacgat
    17881 tttgacttct gcgtgtgcac cttctcctgc taaatctgag tcaaagtcag cgatcacatg
    17941 accgtcattc atgacaccaa ttgcccagtc gacagaagca tctcgtaaaa tgtgcccacg
    18001 gcgattcata taagaagtaa tattttctcc taattgatct acggctgaat atttgatttt
    18061 tgaaccagct ttagcgatga cttccacgat gatattacct gatgattttg ctacttgttc
    18121 tttggttgtc tggaaacgtt ccagataaga aaattcacta tgttcatctg ccacgatcaa
    18181 cacatgcttg aagaagtgct catcggatgc gccatcttga atgaataagg actcgagtgg
    18241 ttcatcgatc acgacatttt taggcacata caggaaaata ccgctgttca taaaagctac
    18301 atggagtgca gtcaattggt cttcgttcat gtttactgct tttttcatat aatattcttg
    18361 gactagttct ggatattcga tcattgcagt gaacaagtcg gtaaaaatga ctcctttttc
    18421 tgctaattcc acaggcagtt gttcaaaaat cgtccatgaa ccttgttgga caagcacagg
    18481 attgtctttc attgcatcaa atgccggaat cgtgcctgtt tcactgatgg tttgtgtttc
    18541 tttcacatca aataacgacc agcgatggaa tttcacccga tcgatatgcg gcaatggcag
    18601 ttcatctgct ttcgctagtg cgtcttgacg gaaggttctc atccaatcag gttcgccttt
    18661 acttaaagaa aaatcattta ccgcgtcaag ataatcgagc cagttttttt ctttcattct
    18721 tgctccccct tacgcttctt cttctttata gtcgattcct aattctttac tgataccagc
    18781 gtagccttct gcttccagac gtttagctag gtcagcattc cctgtcatga ctactcggcc
    18841 ttccatcatg atgtgaacga catcaggtgt gatatagttc aataatcgtt gatagtgggt
    18901 gatgatcaat gcaccgaaat tgtctccgcg catttcattt actcctttag caacgacttt
    18961 caaagcatcg atatccagac ctgaatcgat ttcgtctaaa attgcaaatg taggttctaa
    19021 catcaacaat tgcaggatct cattccgttt cttttctccg ccagaaaaac cttcgttcag
    19081 ataacgttca gccatttctt ctggcatatt tagtaaagcc attttttcat ctaacttttt
    19141 caaaaatgcc ataacagaaa ttttgtcgtt ttcatcacgt ttcgcattga tcgctgcacg
    19201 catgaattcc gcattcgtga tacctggtat ctcacttgga tattgcatag ctaaaaataa
    19261 gccaagtcgc gcacgttcat ctacttccaa gtctaaaaca tttttcccgt caaacaaaat
    19321 ttcgccttct gttacttcat agtttggatt gcccatgata gcggcagata aggtagattt
    19381 tcctgtgccg ttaggtccca tgatggcatg gatttctccg gttttcattg ttaggtttac
    19441 gcctttaagg atttcttttt cctcgatcga cacatgtaga ttttttattt ctaagacaga
    19501 catgcattat acccccaaat tctctttcgt tctcactcat tttagactaa ttgagaatga
    19561 agcgcaatga ccttccttac taaaatcaaa tatcttttat ttattattaa acaattttga
    19621 tttctaaatt agaataggta taaaaaagat gagaaataag ccattacaga cttatttctc
    19681 atcttttttg ttccatacct tattttgcat cagcctttac tggtttaaca gagccgcccc
    19741 atttgtctgt gatccattct tggacttctt tgcggtgaag cacttcaatc agttttttga
    19801 ttttgtcact gtcttcatca cctttacgag tcgcaataat attcacgtaa ggagagttgt
    19861 ccttttcaag caatacggca tcttctaatg gattcaaccc tgcgccgtaa gcgaagtttg
    19921 cgttgattgc aactaggtca ccttcatcat tttggtacgt agaagctaaa agtgatgggt
    19981 cgatcgtatg gttgaacttc aagttcttag ggttttcttc aatatcatcg aatgttgctg
    20041 tttccagatc tactccatct ttgactttga ctaagccagc atcttgtaga attgtgatga
    20101 tgcgtcccca gtctgattca gaatttgaag taatgattgt tgcaccatct tttaattcgg
    20161 aaacatcttt gactttttta gagtacaagc ccattggttc gatgtggaca gcaccggcat
    20221 tgacaaagtc atatcctttt tctttgattt ctttgttcaa ataaggaata tgctggaagt
    20281 aattggcatc aatgtcgcct tcttccaaag attggttcgg taatacatag tcatcgaatt
    20341 tgacgatttc aagatcgatt ccttcatctt tcaataatgg tttcacatgt tccaaaatct
    20401 ctgcgtgcgg tgtcggtgaa gcgccgactt tcaatgtcgt gtcgtctgct ttatttgctg
    20461 aatctttgct gtcgctgttg ttaccgcatg ctgctaatcc tacagtcagc aaaagcgctg
    20521 ctgcaaatcc aaaaattttc tttttcatat cctacatccc cctatatcct attatctttt
    20581 gtctgttttc ttagttaaga aatcaccgat tccttgaacg ataaatacga ttagtaaaat
    20641 aattaaagtc gcaacgaatg tcactgttag attaccacgc tggtagcctt cctgccaagc
    20701 aagacctcca agtccacctg cgccgatcgc gccggccatc gcagtaaatc cgatcatcga
    20761 gatcgttgta actgttaatc cagaaatcaa tgcgggaata ctttcaggta tcaagacctt
    20821 ccagatgatt tcccagtaac ttgctcccat tgcatctgct gcttccagta caccagggct
    20881 gacttcacga aaagcaatct ctactagacg ggcataaaat ggtgctgcgg ataaaacaag
    20941 agctggaatg gctgcttgcg cacctagcat agtgcctacg attgctttag taaatggaat
    21001 gatcaaaacc atcaggatca taaatggcgt tgaccggaaa atattactga tgatcgatac
    21061 gattccataa atgattcgag catacggttt gtttttgcgt ccaatagagt aaaggatcaa
    21121 tcccaaaact aatccgatga tcgtcactaa gaccattgag attaatgtca tatatagtgt
    21181 atcaaggata gcctgattca tcgtttccgg gttgatttgt ttaaaatcta gatatgtttc
    21241 tgaaaagctt ttatccacga tggatcacct caattcctac tccttgttct ttgatgaaca
    21301 cttctgcttg atctaattct ttggtttcac cagtcatcaa gacagtcaat gaaccaaagg
    21361 aaccttcttt cacttgtttg atctttccat acacaacgtt gacatcgatg ttatagcgtc
    21421 gaatcgcttg ggaaatgact ggctcgttgg catttctttc gctaaaggtc aaagttgcca
    21481 aacggccgct tggattttct ttgatgagtt cagctaatag ttctgtcgaa tcagcttctg
    21541 gttcgatgtc ttgttgtaca aagcgtttgg ttacttcttt tttcggttgt ctgaagacag
    21601 tcaatacgtc tccttcttct acgactttcc ccaactccat cacagcgact ttgttgcaga
    21661 ttttgcggat cacattcatt tcatgtgtga tcaagacgat ggtcaaattc aatttcttat
    21721 tgatcgctaa caataagtct aatacttctt ctgttgtttg cgggtccaat gcgctagtag
    21781 cctcatcaca aagcaggatc tcaggatcat ttgccaatgc tcgtgcgata ccgacgcgtt
    21841 gtttctgtcc gccggaaagt tgtgaaggat atgcttgtcc gcgtccttct aacccgacta
    21901 aacgcaacag ttcttctgcc cgttctttac gtttgctctt tggtacgcca gctaattcca
    21961 gcggcagctg gatattttca agtacagttc ttgaccataa caggttgaaa tgctggaaga
    22021 tcataccgat tttctttcgg aatgtacgta attctttgtt ttttaatttt gtaatatctt
    22081 gatcgtttac gatcacttcg ccgctcgtgg gaagctcaag tccattcagg agacggacta
    22141 atgtactttt tcctgcgcca gaatagccga cgattccata aatatcccct ttttcaacgt
    22201 ttagtgaaac gtcattgacg gcttggatgt ttccgttttt cccattgaat gtctttttga
    22261 cattcgttag ttggattaat gacatgccat tcacatcttt ctcttatgat ttttcaaaca
    22321 aaaaagcctt cgccctcttg ccttaagcaa aaggacgaaa gcttgaactt ccgtgttacc
    22381 accttgattt accgttactt cgcaataaca gcctcttcca gtacttggac ttagcttctt
    22441 tccgtatact gtcgcgctgt aacgggcact cccgtagtaa gtttgcttac gctcgcttac
    22501 tctgctctaa gaccatcttc catctcattt tcgttaccca ttttcagcaa acagggctct
    22561 cttcaaacgt ccgtgacatg tactcttctt ttcaaagctt tctcgtttca tataggattg
    22621 cttatgatta tagcaactgg aagaatcagt gtcaactttt ttcaagtgat tttctgattt
    22681 tataattcgt caaattcttt tggatcaaca tctttgaaac ttaagatcca cgcattcatt
    22741 tcatctgaat cattcaagat attgatatct tcaccgattt tatcattaac agaagaaact
    22801 gtacccgtta gcgggctggc aaattcattg accgcttttt ccgcttcaac ttcgatcaga
    22861 ggttcatctt tttttagcga ttgtcctgct tttggcagat tggcaaacgt gatgtctcca
    22921 agttcttctt gcgcttcatt tgtcaaacca acacagtatt cttttccatt aaataagatc
    22981 caaaggttgt cctttttctt caaatgcttg cctgccattt tatatcagtt ctccttcata
    23041 aattttttca ttgaatccaa ttgcaacaag ctggtcctct cgtaagatca gtggtcgctt
    23101 gatcagcata ccatctgtcg ataagacttg gctcgctttt gaagccgtaa actcgcctac
    23161 ttgatctttc agtccaagtt cccgatattt catcccgctt gtgttgaaca ggcgacggat
    23221 tggtacttga ctcgtttcta gccaagtctc aatcgttgag gccttcggta cttctttgat
    23281 catatcaacg gtttgataac taacgccatg ctggtctaac cacgctttgg cttttttgca
    23341 tgttgaacat ttcgggtacc agtaaaaggt gtacaatttt cttacctcct tgaagatctt
    23401 gcaggtgctt cattttggta tctcatcgat aatatcaacc cgataccgat catgttccct
    23461 aaaatcgagg atcctccctg actaatgaaa ggcaatggga tccctgttaa aggaagtaat
    23521 ccgatatttg ctccaatatt ctcaaagaca tggaacagca gcatcatgat caaaccgctt
    23581 gcgatatagg cgtaaaattc attattcgta tcgaaacaca cccgaatcat acgatagatt
    23641 aaaataaaat acaagaaaat gacgaatgca ccaccgataa agccgaagtt ttcaccgata
    23701 acggtaaaaa tcatatcgga ctcacgaaca ggcacataga tattgcttac gttgaatcct
    23761 ttaccaaaca agccaccggt accaattgcc aaaagtcctt gggccggctg ataactcatt
    23821 cctgacgtat catggaaagg gtccagccat gaatcaatac gtgcaaactg ataagatttg
    23881 aatccaactt tataaagcaa gtctcttccg ccttcagtcg taaccaagaa gatcgttcct
    23941 gctccaacga ctactgccaa gattactaca ggtgcgatga tgcgccaaga gattccagac
    24001 atcaggaaaa tcccgccaaa gatcgcaaga aagacaagca tcgttccaaa gtcatcttgg
    24061 gccatgatca atgctaaaac cggtattgtc acgatcaaga gcttcccaat cagccaaaag
    24121 tccgatttca tcgtccgttc ttttacttgc gtattatgtt tagttacgat cagtgccatc
    24181 atcaagatgt acgcaatctt catcaactct gctggctgga aagtaaacgc accgaatcga
    24241 aaccagtttt tagaccctgt tgaagcagct aaattctgat catagaattt gagcaagagt
    24301 cccattacca ctaaccccaa accataaagg taaggagtca gtttccagat ccatttggaa
    24361 ttcaaatgca tgatgacaac gattgctgcc gctccaacta aataccagac aacttgcatc
    24421 cctacgcctt tcaagactgc tggattgttc ggatcatggc taagtgctac atataatgag
    24481 agcagaccaa tcaaacataa cagaaagact ggtaaaatca cgccataatc aatacgatta
    24541 tcgttcgaag cttttaattt tttttccatg tttcatccct tttcttaaaa accatactgc
    24601 tgattattat agcggtccag ttagataatg gaaagtcttt ccatagacat ttgggaaaaa
    24661 ttaactttcc gtttgtgcag ttgcttgagc aaatgcagta caaaagccta ctgcttatct
    24721 ttagtgtatc atagaacttt ttttgataca tgcaattatc taccagctga gattctcctc
    24781 gctgtgagaa gtttctgtgc tttcattgta tcgaaggatc tcttcggcaa tctctagcag
    24841 ctgatcagaa accagattgg ttcgttggat gaaataaaaa tatcgatgga aattcaacgg
    24901 agtcattggc acattttctg gaacagagcg cttagacaaa atcgagcgac cgattccttc
    24961 cttcagcaac gcaacaatta catcgttact tttcacgatc atcttcttat cttggatatt
    25021 catctcttca aaataacgct gcgtgtaatg aaaaacacca gagtttgctt ctcttacaag
    25081 ccacaatttg ctttttgggt ccccagcgat gactagctgg tcttccatca aagagtatct
    25141 ttgtacgccg gaagtctcta atggcttttc gataaaacca aaatcggatt catgtttcgc
    25201 tacttgttct aatgattcat gagaattcgt gatttcgatc tcaaaatgca ctttaggaaa
    25261 ccttttgatc agatgtttca tcaactgagg caaaagatag acagcaaagg tgtgggaagc
    25321 aacgatccga catacttctt cttcattttg ttcattgagc aatttttgct gagtttcttc
    25381 ccattcttct aacaaaacca gtgtccgttc atataataag tcggcttgct tagttggaag
    25441 catctctttt cttccgttac gttgaaaaag atcgatattc aattcccgct ccaactgttt
    25501 gatttgattc gagactgctg gttgagataa aaacagtact tctgctgctc tggaaaaatt
    25561 tcttgtttca tatactgctc gaaaagtcgt taataactta aacatggcac cacttccata
    25621 actttttatg ataattacta taaaaactat ttgatttaaa tatacaaaaa accatctata
    25681 atagaaatgt acaacaaaac agttgctgat ccaactagta acttagatga agacaatcag
    25741 ctgtctgctt gctcgtaatc atacattcct attcatttta caatgtattc gttcatcttg
    25801 gtactaaaat ctattaaata aaaaggagaa tattatatat gaagaacttt ttgaaaatta
    25861 ttccgatccc gatctgcggg ttgatccttg ggttagcttc ccttggaaat ctgtttaaag
    25921 actatcacca tgttgcttta ggcaatctaa ctggcggtat ctctatgatt cttatgatat
    25981 tcatttttgt taaattgttt atcttattcg aacacacgaa acaaacgttg catgatccaa
    26041 tcatcgcctc cgtttcacca acttttacga tgtcactcat ggttatttgt acttattttg
    26101 tttcatatga aagtattgca ccaattgtca aattcatttg gctgtgcgct gttatcttcc
    26161 aagtgatcct tgtcttttat ttcaattatc accatgttat caaagctgat ctttcaattg
    26221 aagcaattta tccaagctgg tttatcattt acgttggatt tggggtcatc actgtaactg
    26281 ccgggaactt cttcccactt attggaaaaa ttttcttttg gatttcattg atctgctatg
    26341 cgttcttatt gccgatcatc atcaaacgtg tctttttcgt aagaaatatg gctcatccga
    26401 cacgtccgct gattacgatc gttgcagcac ctggttctct ttgcctaaca ggttatctta
    26461 aaaactttgc tgaacctaat ttttatcttg tcgtagcatt gttcgcttta tctcaagtat
    26521 tgtatttcgt tgtgctgtct atgcttccac gtttgttcaa gcttcgtttt tatccaagct
    26581 atgcagcctt tacattcccg cttgttatct ctgcaacagc tatatttact actgttcatt
    26641 acttcaacag tttaggtatc tatagtagcg tattggattt cttaaaagtt gcggaatgtc
    26701 tgtttgcaag tgtagttgtc ttctacgttc ttgctcatta cgtacgtttc ttaattaaag
    26761 aacatagcaa acatggaaaa tttgtttcta aaaaaatcgc ttaataatcg attcaatagg
    26821 ttgtgaaaaa gctgttctgc atcaagtaat aagaacggcc aaacaaaaac agctgttcat
    26881 gtttttgtac tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaccaag
    26941 aggtcgtaaa agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct
    27001 caaatttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac accgtttatt
    27061 cgaaacaaga ggtcgtacaa gacttttgtc acgacctctt gttcttttgt caaaatactg
    27121 gtttagtgct actatgttcg agtatgcttt tagtatttat tgacgaaagg aaccgatcaa
    27181 atgaaatcat tgtcacatcc aataaaatct gtaaaggatt cagcggtccg cgaaatctta
    27241 cccggtttac tgctttcttt tgccatcgct gccttcagca ggcttcttgc tatttatctg
    27301 ccacagttag gtggagcgac tattgctatt ttattgggta ttttattagg aaatactttt
    27361 tttaaacagc ctgttctagc aatcggcaca aaatttgctg aaagcaaact attagaattc
    27421 tctgtttttt tactaggtgc aactgtcaca gctcaaacga ttgctcaaat tggtgtaaaa
    27481 ggaattgttt ttgtgttact gcaaatgtca gtcactatca tcggtgctta cttgattggt
    27541 aaaaaactgc aattcagcga tacttcttct ttgatgatgg ctggaggaaa cgctgtttgt
    27601 ggttcttctg ctattgcttc aatcgcccct tccatccatg cgaaagagga agaaaaagga
    27661 cagatcatca cactagtcaa tctactgggt actgtgatga tgttgactct cccttttctt
    27721 ggattacagt tgtttggtgg aaatctcatt gaaaaaagtg cacttttggg tggtacactc
    27781 caatctgttg gtcaagtagt cgctggggca agcttacttg acaattctgt tgtcgaattc
    27841 gccatgctat ttaagattct acgtattatg atgctcgttg ttgttgtcta tctgtttgaa
    27901 aaattcatgc acaagaaaca accagctctg tttgaaacgc caactgatac gcagaaaaaa
    27961 cgaaaattac cgcttccttg gtatgtatta gggtttgtga tcttttgttt ggtcaacagc
    28021 ttgttttctt tacctgtagt tattgaccaa tcggcacatt ttatcagcag ctggtttgag
    28081 atcactgctt tagcggcaat tggtctgcgt ttagactttg caaaattcat gaaagaaggt
    28141 acacgcttct tatcttatgg attagctgtc gggcttctcc aaacagtgat ggctttacta
    28201 tttatttggc tgatcaaagt ttagaatgaa catgaaaaaa cagttgtgat tctcgcatgg
    28261 aacaatgtcc gaatcacaac tgtttttaat tttgcagata tccttctaac aacaacaatt
    28321 ggcgtttgat ttcaacccca tctattgcgt ttcccatata tcctgtcagt tttccatttt
    28381 gcccgatcac tcgatgacaa ggaataacga ttggcaatgg attggttcga tttgcctgcc
    28441 caatcgctcg cacagctttc ggactatcaa cagcaacggc tacttcttta tacgttcgga
    28501 tttctccata aggaatggtc gacaaagctt tccagacatt ttgctgaaaa ggcgtacctc
    28561 tctttataga taaaggaaca tcaaattctt ttctttttcc cgcaaaatat tcttcaaact
    28621 gttcttgtgt gcgcgttaaa attcgctgat tcgctgattc gctttctgaa attggctgaa
    28681 aagatacagc agtcaatccg gattcatctg catctaacca taaaaagccg ataggtgtgt
    28741 gtattttcat tttgtttgct cctagtcaat agactggaac ttcctccttt ttctctatcc
    28801 aaaactactt gccaactaat aaaaattgtt cttaaaagtt tgataaaccg aggaaacatg
    28861 gtaaactaaa tacgttagtt tacaaaaaga atacctgttc ttttacgtaa aatcaaattt
    28921 taaaggtgtt atttaaggtt tattaaattt tgaatgtaaa ccatatataa acatacgatt
    28981 gaaaggatag aaacgatgaa agtatcaatt gttgtcccat gttttaatga agaaaaaact
    29041 gttcctcttt tttttgatga ggttgaaaaa ttcaaaggtg cccatgagtt cgaatatttg
    29101 tttattgacg acggatcaaa agacggtacg ttagcagcaa tcaagaagct agcctcagct
    29161 tatccttatg tccgctttgt ctcattttcc agaaattttg ggaaagaagc ggctctgtac
    29221 gctggattgc aagcttctac aggagattta gtgaccgtga tggatgttga tctgcaagat
    29281 ccgcccgaac tgcttcctga gatgatcaac cggatcacta cttctgatat cgattgtgtc
    29341 ggcacccggc gttcgactcg tgaaggcgag cctgcgatac gcagtttctt tgcgaaaaaa
    29401 ttctaccagt tgatcaataa aatcagcgat acagaaatgg tcgatggcgc tcgtgatttc
    29461 cgcttgatga ctcgtcaaat ggtggatgcc gtcctagaat taacagaata caaccgattt
    29521 tcaaaaggat tatttagctg ggtcggattt aaaacagaat atatttctta tgaaaaccgc
    29581 gaacgtgtgg ctggcgaaac atcttggtcc ttctggaaat tattcaacta ctcgattgat
    29641 ggtatcgtga acttctcgga tgcaccgtta acgattgctt catttatcgg cgctttgtct
    29701 tgtgtcggtt ctggtattgc aattttgttc attattttgc gggcattgtt gtttggtgat
    29761 cctacatcag gatggccttc catggtctcg atcttcctgt tcattggtgg aatccaattg
    29821 ttatgtcttg gaatcatcgg gaaatatatt gggaaaatct tcttagaaac gaaacagcgt
    29881 cctgtttata tcataagaga aacagaaaaa gtgaagccaa atgaattgaa gcctcaccaa
    29941 taatatacgt caaatgaagt aaacattttt ttaaatgaaa aaggactgtg acaaaagtct
    30001 tgtcacagtc cttttttccg aataaatggt gttccagaag tagcttcttc ggaaataagc
    30061 cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    30121 gaccacttct atcacaacct ctttcatatc ttattacttc ttcattgctg cttttagtgc
    30181 tgctgctaat gggctttctt ctggttcttc tgcttgtgca tattttttca gcaagcgtcg
    30241 ttcttcatgt ttggtcattt tcttcttctg ctctttttta tcaagcaatt tttctgtcgt
    30301 tccatcgtat ttacaacgga aataagcacc gtttttacct tcgatgattt ccattttccg
    30361 gtgacattgc gggcatcgat gattagagac ttttggatct tttcttctgc ggtagttgca
    30421 atctgaattg gtgcagacat agattttgcc gtcacgtgtg tttttctcgc gcagattggc
    30481 cccacattct gggcatttct tttgggtaat cgagaaatct tgatattctt gtttgcttga
    30541 tttgatctca ttcaccaagc gttttgtttc tttttcgatt ccattcaaga aggttcctgc
    30601 ttgtttcttt cctgcagcaa tagcttcaag gtctttttcc cagctttctg tcaaatctgg
    30661 tgttactaaa gagggattta ccaatactaa taattgctta cctttaggag atacttgcag
    30721 tccttgaggg gtacgttcca tcagttcaga tttgattagt ttctcgataa tctctgcacg
    30781 cgtagctggg gttcccagac tgtatttttc catttgacca agcaagcttg cttctgacaa
    30841 tggtttaggc ggtgcagata gttctttttt gatggcgaaa tcgggattga ttctcattcc
    30901 tttttcccat ttgactgttt ccgtctcagt ttcagtttcg cctttccatc ctgcttggac
    30961 gacacgattt tgccgaaaaa tgaatcgttc tttaccaaat tcaaccgtaa cttttgtttg
    31021 gctgacttta tggggttgag caaacagtcc taaaaaacga gagacaatca tttgatagat
    31081 ttttcgctct tcattactta atttttcata acgtgctctg ttttcagttg gcaaaagtgc
    31141 atggtgatcg gtcactttac tgtcttggaa tacttttgtc tgttggacga tggctccgtt
    31201 tttcaaatac tgcttgacct caggagcaaa gtcgctgact gcttgcaatc gttctttcat
    31261 cgtacttttc atgtcatttg tcagatattt gctgtctgta cgcggataag taacgatctt
    31321 atgggtctca tacaaacttt gtacaagact taatgttttc tttgcagaat agccatagcg
    31381 ttgattggct tctctttgga tctctgttaa atcataaggc aatggcgcag attccgtttt
    31441 taatttttct tggacttctg tgacagttcc tttgcttgta ctgatcaatt gaacaagttt
    31501 ttgtgcttct tcttgtgttt ttaaagcaaa ttgatttttt tgaagcattt tggcttgttc
    31561 ttttccagct tgcaacgtga tcgtgaaata cgtttgtgga cggaactgct cgattttctt
    31621 ttcttggtct ctcactaatg ctagtgtcgg tgtctgcaca cggcctgctg ataaattatc
    31681 cgcgtacttc acagttaagg ctctggtcac attcaatcct accagccagt ccgcttttgc
    31741 ccgggcaaga gcagattcat acaatgcatc atattctttc gcaggtctta gttgcttgaa
    31801 tccagttttg attgctttat ctgtttgaga agagatccat aaccgcttca ctggtttatt
    31861 gaaatgcaca tactctaaga tccagcgagc aacaagttcc ccttctcttc cggcatctgt
    31921 cgcaatgact gcttctgaga catcttttcg ctgagccagc tgcttgattg cttttaattg
    31981 atgtcctgtt ttaggaagtg gtttgattcc tagtttttta gggatcattg gcaacgtgtc
    32041 catttgccag ctctgccatt ccttattcag atcttccggc attttcaatc ctaataaatg
    32101 accaagggcc caagtaacga tcaccttggg tccttcataa taatttttgt gcttttgatt
    32161 agcaccaagc accttgctta ggtctttcgc aacgcttggt ttttccgcta gaattagttg
    32221 tttcataaaa ttcctttctg ctttctactg tgatgggctg atgatttttc aacaacgcaa
    32281 gtcccttgta ttgctgtaaa tcatcgtcac actcttcaca ccagcgctca tctccgtcat
    32341 tccaaaaggc gctgagaaag ttactttttg cattgatata ttcataattt tgatggattt
    32401 cgttccattg tttttcgtac gcttgtcgtg cttcttcgaa agactcgaat gtttcttctt
    32461 cttcaatatc ttcttcccag tcttcaaaaa accaccaagg ctcattgtcc ccgaacatgg
    32521 taattacttg atacatactc gtgctccttc tttcagcttt tctagactca tcttagtcac
    32581 ttttcaagaa agttgcaaga attttctctt ataaaagggt catttaatga acatcttaaa
    32641 attgttacaa atgaatctga ttgtttaagg caaagtagcg ataaagttat tgatcatcca
    32701 aaaatagctg tcttctggat cgattgttgg ttcttttgta aagtatccta aaaaacgtaa
    32761 ggaaagataa ccgtggatca tactacgaag ttccctgctt cgatgaactt tttcttcttt
    32821 cgttaattga aaagcctcaa atatctgcaa aatgatctga ttcgtttcat ggattccatc
    32881 taataaaaca gcttcattgg tatgcggtac actgatcagt aactcataca ctgcttgatt
    32941 tgaaaaagca aaggatcgat aagtttctgc atagatctgc agtgcttcct gaccactttt
    33001 tcccacaaga gcccgtcgca gctggtcatt aagttcctgc agaagtttcg ctgttagcgc
    33061 tgttttgaca tcacgaatat ttttgaaatg gttatacagc gagggagcct tgatatccaa
    33121 gcgtttggct aaatgctgaa aggttatttg ctgaacatcc atctcttctg ctagttctcg
    33181 aaagcagtcg atgattttct cttgagacag atttcttttt ttcatttcca tgtttcctct
    33241 cataaaataa atccttcact ctttcagttt accacgaaaa agaaagagaa aaaagagact
    33301 ttcattgata aactacatat tgtagtttat aataaagagc tgtagatgaa aattttcaga
    33361 aagaaggagt ctaattggct gcaagaataa aggaaaaatt atttaggaaa agagaaactt
    33421 gggaacaaaa cttgtttgtc ttatggtttg gaacatttat ggctggtatc ggatttagtt
    33481 tagtcatgcc ttttatgtca ctttacatag atacgttagg taattatagt acgtcacaat
    33541 tgaacttttg gagcggattg actttttcat ctactttctt ggtgactacg cttatctctc
    33601 cttggtgggg aaaattagcc gaccaaaaag gaagaaaatt gatgctgttg cgtgcttcat
    33661 taggaatggc aatcgtcatt tctttgatgg gttgcgtgac tagtgtgtat caattagtcg
    33721 cattgcgtct gcttcaaggg attttttctg gttatatcag taatgccact gcccttgtcg
    33781 caactgggac gccaaaagaa aaaagtggtc aagttcttgg aaccttagca actggttctg
    33841 ttacaggaac attacttgga ccgcttcttg gcgggatctc tgcttcagcg tttggctatc
    33901 gccctacctt tttcattaca gggatcatct tatttttcgt cttcttgttg agtctgtttt
    33961 tcgtccatga aaaatttgtc ccagtgaaaa aagaaaacat ggtttctgcc aaacagattt
    34021 tcaaagactt gaaatatcca cacgtcgtca tcggtatgtt cgtcacaacg atgatcatcc
    34081 aagcatccaa caattccatt agtccaatca tcagtctgta cattcgccaa ttgctgcatg
    34141 gacatgggaa tgttacgttg atcagcggaa tcattgcttc gatacctggg attgccactt
    34201 tgatagccgc tccaagattt ggtcgcttag gtgacaagat tgggagtgaa agaatacttg
    34261 cgattggttt gggttttgcg attctggttt atatcccaat ggcattcgtc acgaatgttt
    34321 ggcaattagc tgccttacgt tttttgattg gtatctctga tgcttgtctg ctccctgctg
    34381 tccaagcatt gatcacaaaa tattcgcctc atcaagctgc tggacggatc ttcagttata
    34441 accaatcttt ccaagcaact ggtaacgtag tagggcctat gatcggctcc agtgtttcca
    34501 gtgcatttgg ttatcgtggc gtctttcttt cgacttctct tcttgttttg gtcaacttct
    34561 tgcttgtacg acacaataca caagagatcc atcagcaaga aactgcagaa gaaaaagctt
    34621 caggagtctc catagcttca gggcactctc cttctcatag acaataagcg taaatgaaaa
    34681 acactgcttg aacagttgtc acagactgtc cttgcagtgt ttttgtttag gcctgttttt
    34741 tcaagaactc agataagagg aataatccct tttccaaggt ttctttttta caacagtaac
    34801 ctagtcgggc atgcttcggt aaatcaaagg cacttcctgg tactaatagt actcctgttt
    34861 cttctaataa attgatacaa aatgtctggt catcttcttc gatggctaat ttaatgaaag
    34921 aagtagagac gtaattggga gcgacaagtt caactttcgg ttcattggct acccattgtt
    34981 ttaaaatcac aaggttctct gtaatgatct ttttgtttcg cgccaatatt ttttctttat
    35041 tcttcaatgc atgtacagca agatcatctg acaggactcc gccgcaaatc atcgtgtagt
    35101 ctcgatactt tcgaaaaact tctgccaact caggtccagt agctgtccag ccaatacgaa
    35161 tacctggaat ggagtaggtt ttggataggg aattggttgc tatacctttt tcatagacat
    35221 ctacgatcga caagaattca cctttatccg ttaaaggcgc atatacttca tctatcaata
    35281 catatgcatc tactgtacga gcaatttctg cgattttttc taaagttttc cgatctagca
    35341 atgtgcctgt aggattgttt gcactattca aacagatgat ttttgtttca ggtttgacta
    35401 aggcttctag ctgttctaaa tcaaactgcc aatcttcttc ttccttcaaa tgaacaaagt
    35461 caacagcggc acctaaagat ttggggatgt catataattg ctgataagta ggaagcatcg
    35521 aaacaacatg gtctcctgct tccactaacg cgtatagagc taaaagatta gcaccagtcg
    35581 ctccattcgt ttgtaagatg ttttccggat cgacggtttg ataaagagaa gcgacttctt
    35641 gtttgaaaac atctgatcct tcgatccagc catagtccaa tggttcgttt ttgcgttttt
    35701 cgaaaaattt acttacgcta gtcccatcta agccaacgag ttcgtctaat gtcaatgctt
    35761 cgatcgagct ttgtgcaata tcatataccg ctttcttttc ccatttattt agccattctt
    35821 ctacaccgaa atttgcgatt ttcatgtttc tttccccctt atatcttgat ccagcctaca
    35881 gcaagcaacc cgattgaaag cagtgcggcc acacaaatca gcccaatcat tactttttct
    35941 tttcccgtaa tttgatgttt ggtttcacga caagcgatcc agtagaagca aaaaccagga
    36001 agataggcaa tactacataa aaggacataa gaaaatcctg ctaaaatgat gcccatcaat
    36061 tcaaaaacta cagcaatccc accgatcact acttgttttt tgattccttc ttttaaagaa
    36121 aactggactt gatagatccc gacaagtaga taacaaatca aaattgccgc tgtacataaa
    36181 gaataggcaa attgataggc ataatcagta actaagaaag tcaataagaa tagattagtc
    36241 aagattgcag taacaactaa cgaataagtt ggggcatttt ttttattcgt ttttccccaa
    36301 cctttcggca acagaccgtc tcgagccatc aacatcgttg tttctgctgg gagcatcgtc
    36361 caagacaacc aagaaccaat gatcgaaata atcaagccga tattgatcag cgctgctccc
    36421 caatttccca cgattccttt taaaatattt gccatagcag gttgtgaaat agttgctaac
    36481 tcttcttgag ataagacgcc gtatggcaag atcgaagcaa ggatatagat aaacaataaa
    36541 caaagcagac caatgatcga tgcttgctgg gcttctgaac gtttcttcgc gcggttggct
    36601 aaaacacttg cgccttcgat ccctacaaag acccacatca taaccatcaa acaaccttta
    36661 atctgcaacc agatcgttcc ttgctgccct gttccattca ctagattatc agatacgtta
    36721 ccccaaaaat cagcagtgaa tatattcact ttaaatgcga taaatacaaa tattaaaaat
    36781 aggaataatg gcactaattt acagatcgtc acgattgtat tgatgacgct cgctccttca
    36841 actccattgt ttactaataa agtcagaccc caaataaaaa tacttgcaaa taaaatagaa
    36901 ggaacatttt gtccaccttt gaatatcggg aaaaattctc ccatggcgct catcagcatc
    36961 gttgcaaaag caacatttcc cagccacgca gacaaccagt aaccccaacc actgatgaag
    37021 ccacctaatt taccaaatga agcttccgcg taaccgaaaa taccaccatc tagatctgga
    37081 cgcttttctc ctaaatgatt cagcgataat accaaagcta aaataccaat cccaacgatt
    37141 atccatgaaa ggatcgctgg ccctggagct gcagaactag ccagatcact tgtgatccca
    37201 aacacaccgc ttccgattga cgaactgaca accagagcaa ccagtcccca tttgctaata
    37261 cccgtcgatt tcttttccat ttccatcacc ctcacttata aaattgaaca aatcacttgc
    37321 aaaagattcc cttctatttt gcaactgctc ttgcttaaca aaaagcaact ttattcatat
    37381 tattggatat tatgaacttt ttacacaagt atttcaagta taaaatgaat aaaaattaat
    37441 tttattgcaa aaaaacagct atgctatcca aaagaaaagg tgtttctttt gttagccata
    37501 gctgttttga ttcatttaat atcgcttaaa cgtattgtaa aacttttgtt tcttgatatg
    37561 cgtggtcgat caaaccacca cccagacatt ccattccatc ataaaataca actgcttgtc
    37621 caggagtgat tgcacgtaca ggttcgtcga atacgacttc tgcacgatta tcatccaata
    37681 agcgaactgt cacaggaacg tcttgctgac gataacggaa tttcgctgta cacttgaatt
    37741 cttttggcat tggttcattc gtcgtaaagt ggatttcact tgcatccaag cttgtggcat
    37801 acaatgcagg atgatggaac ccttgcccca catataatgt gtttgtcgct agatcttttc
    37861 ctacaacaaa ccagggttct tgcgaatctc ctccgccacc gatccctagt ccttgacgtt
    37921 gaccaatcgt ataatacatc aagccagcat gttgtccttt gacttcacca tcaagagtga
    37981 ccatatttcc tttttttgct ggcagatagt tgctcaaaaa ttgtttgaag ttcttttcac
    38041 caataaagca gattcccgtt gaatcttttt tcttagcagt cgcaaggcct gcgcgttcag
    38101 caattgcccg tacttctgat ttttccatgc cgcctagagg gaacatcgtt tttgaaagct
    38161 gttcttgtga taattggcta aggaagtaag tttgatcttt attattgtct actccacgaa
    38221 gcatgtggac cacgccgttt tcatcgcgag tgacttgcgc ataatgtcca gtagctacat
    38281 aatcggcgcc aagatccatt gcataatcca agaatgcttt gaatttgatt tctttgttgc
    38341 acatcacgtc tgggttaggt gtacgtcctg cgcggtattc ggctaggaaa tattcaaata
    38401 cacgatccca atattctttt tcaaaattca cagagtaata aggaatcccg atttgagcag
    38461 ctactttcgc tacatcctta taatcttccg tggccgtgca tacaccgttt tcgtccgtgt
    38521 catcccaatt tttcatgaat attccgatca cgtcgtatcc ttgttccttt aacaaaagtg
    38581 ctgttacaga tgagtcgaca ccgccgctca tccctacgac aacgcgtgtt ttgctgttgt
    38641 ctgtcatgat atcaccatca tttcaattag attctgaaac atctacgagt tgaaggtcgc
    38701 aacttttcag tattttacat tatacgtcat atttctgaaa atttcatccc ttaattatca
    38761 agtcgtaaaa atactgttct actggaccta ttcagtacac aaacatgata gtcaaccaaa
    38821 aaatcactca tcttttgagc attcgatata aaaaattggc atactcataa aaagggccag
    38881 cctttcggca ataaggaata attttataaa ttgtgaaaaa ctatttttca aaattgcttc
    38941 ttatcgctta aagctgcacg tctcatttac aaccttttct ttttagatgt tatgagttgc
    39001 tcagataagt gattttgttt actctaagaa aaaagggagg caaaagatac atgaattact
    39061 ttatcagtga tatgcacttt ttccatgaac acttattagg aaaaaacgac tttgctccgc
    39121 ggctctttgc aaatgtggat gaaatgaacc aacagttgat cactagctgg aatgcagtaa
    39181 taaaagaaac cgaccatgtt tatcatctag gcgaccttgc tatgcatccg caatatgaaa
    39241 aagcaaatgc agaaattttg gaactggtca ctcaactgca aggaacaatc cacttgatca
    39301 aagggaacca tgacagtcgc gcttttttcc gttatttaga aaaaaatgat cctaggctta
    39361 ctgacggaag tcccaaattt tatttttatg atgtaggtgc catcgttaaa ttcgaccatc
    39421 aacaatatta cttgactcat tatccgatgt tgctcgggcc taacgacact atccgcaatc
    39481 ttcatggaca tatccatcat tacagcgtac ctatcgagaa cgatgtcaat gtaggcgtag
    39541 acgcaccgga aagagaactc ttaaaggaaa aactaccatt tggcactccg ttatctgagc
    39601 aacaaatcaa agaaatctat gaagcaaaga ggaagtacga taggtcgtga aaaaactgcc
    39661 ctatatcaag tattaaacaa ataaagataa ttactcatat ttttcatttg agtaggctta
    39721 ttgccacagg aacattagga tcagaaatgg agttgtaaca agacttctgt cacaactcca
    39781 ttccatacta ttcgtctttt tgctcatggt gttcttttac cagttttttc tgtaattctt
    39841 tatcataatt tcgggcagct gacgttaaaa ataaaacgat tccgcataat aaaatcccga
    39901 ttccggaaaa caagaacatt ttttctaccc ctaaacgatc agctaatggt cctgcaaata
    39961 ataatccaat tggacctgcc aggttcatca aagagttgaa gttccctaag acacgaccta
    40021 aaacattcga ttcataactc tgctggatca ttgccatcag cagtgtattg aaatatggcg
    40081 ttgcacaacc tgctcctgca ttcaagataa gaaagtacaa gaacccttga ctatcgtttg
    40141 gaaccaatcc gcttgcaccg atcgtcaggc ccataatgat gtaggctata ataataggtt
    40201 tcattcggtc tttccatgtt cctataaaac tgatcagtgc accgcctaag agcattccga
    40261 ctgcgtagac tacttccact gctcccgcct gtccaacgga accaccaaag taatctagcg
    40321 tcatcaatgg atacagactg attgctggca tgaacaacag catcacaaat gcaccattga
    40381 tcgttatgta ccataaccct ttattatcca tcaattgctg cagcccaaat tttgcatctt
    40441 taaaaatagt aagcggaact tccaatattt caggagaaac ttttgggatc ttcacaaaaa
    40501 tcaataagcc gacaccaaaa actgccccca atacatctaa taaaataagg taattgacag
    40561 gaacgactga aaacaaagcc gcacctagag ctggcgcaat gatgaaattc gctgattgga
    40621 ccattcctaa ctgtccattc gtccttgtta tgtgagaaga tggaacgatt gtcggcataa
    40681 tcgactggat cgttggcatt tggaatgttt gtgcgaccga acgcataaac aaagacacaa
    40741 aaactagcca aatcgggaag gattctgaaa tcgttccgac tactgctaaa ataagcgcga
    40801 aaatcgctac aataatatcc gtgacaatca aaagagcttt tttatcccag cgatccacta
    40861 gcggtcctac aaatgggctt agtaaaacca tgggaatcat tcctagtaaa gtagcaaaac
    40921 tcaaaattgt cgccgaaccg gtttctcttg tcaaatacca gatgatcgca tattggacga
    40981 ccatactagt gattcctgac aaaaactgtc ccgataaaaa gaacaaataa tttctcttcc
    41041 aattcgttaa aacgaccgac tgttcatttt ccataaacat ccctcttttc tttttcattt
    41101 tgtatagcgt ttgcacaaca atcattatac gggattcttt tttcaaaaaa tacctttttc
    41161 cctaaataac aatcgtttca atttaaattt ctttacttag aaacaataaa tcagcaaaat
    41221 attaaaaata acaacatgtg ttaaatttaa aaataaaaaa gacaactaat ttgcgtcgtc
    41281 cttacaaagt tagtcatcta acaataggcc gtttcaaaac tagttgtctt tttatatcca
    41341 cagatcattc gactttttcg aataacccac ggctaatcat cccatccatc agaaaataaa
    41401 atttatcttg gctcattttc tgttttggat ctttgaaaat attgactgct ttcaatccat
    41461 ttgctgtagt gatcgacatt ttcaatgttt gaatatcttt tgatacagtg atttttaatt
    41521 tgaatccttc tttgctttgc ggcgtggcat ctaatggtcg gatcaattga taattgcctc
    41581 cgtttgtttc tgtaaatcca ttatcgcgca atgtatatct ttttgcatca ggatgcaaac
    41641 gatagctaat tggtgcgccc agtaccgttg ctgttgtttc aaatgccatg attttcacct
    41701 cgataaaatt agtttctctt tattataaaa gtttatcctg atttttacta ggtttttcag
    41761 ttaagttccc taaaaaatga aggcgttctc tcagacatct gttcatataa gaatgacttc
    41821 actagttatt ttttgacagc acgaactaat tgatcaagga aatactggat gtcgtcttcg
    41881 gtatttccca aaccaaagct gatacggatg gattctttta gtatcggtgt atcttctcca
    41941 tacattgctt ctaatacgtg tgaaggctcg atatttcctg ctgtacaagc tgaaccagta
    42001 gaaatagcga ccccattaag atcaagcttc atcagtagca aatcatttgc ttcgccaatt
    42061 aagcgaagat tcaacacatg agggagttta ttcgttaaat caccatttac ttgataaggg
    42121 atttcgtttg cttctaatgt atcaagaatc aattgctgga atcctttata tttgttgtta
    42181 cgtgcttcac gttcttcagg tgacagcagt tcaactgctt ttgccatacc ccagatccct
    42241 gctaaatttt ctgtacctgc acgccgcttt tcttcctgct ccccgcctaa aagcaatgca
    42301 gggatattta ctccgtctcg tttatataag aaaccgacgc cttttggccc attgatttta
    42361 tgagcagaga tacttaataa atcgattcct aattcttctg gatggatcgt ttggtttccg
    42421 tacgcttgta ccgcatctgt atggaaataa gctggatgat ccgctaaaag ttcaccgatt
    42481 tctttgattg gcatcaaatt tccgatttca ttgttcccgt acatcacaga gaccaagatc
    42541 gtatcttctc gtaacgcttt tttgacctct tcgatcgaca gattcccttt ttcatctaca
    42601 ggcagatatg taacgtcaaa cccctttgtc tctaaatatt tcatggtatt aagaacagca
    42661 ggatgttcga tagctgtagt gattagatgt ctgccttctt tttgacgcga gaacgctgtt
    42721 tctaagatag ctgtattatc cccttctgtt cccccactgt taaagatgat ctcgtgaggt
    42781 tttgcttgca gactgtctgc aatgatttgt cttgctgttt ccaatttttc atgcgcgtgg
    42841 cgaccaaatc catgtatgct tgaaggattt ccaaacgtgt cttgcatgat tgccgtcatc
    42901 gcttcaatta cttgtggatg catgggggta gttgccgcat gatccagata aatagttttc
    42961 acttcattca tcccttccaa attattgcta ggcacattct agcataaaat agcgaacaaa
    43021 ccaaagaaga gctcattttt gtctagagga cgtttcatgg gcatagaaaa aaggagctgt
    43081 gacaaaagtc ttgtcgcagc tccttgaagc tgctcacttc tgtcacaact tctttccctc
    43141 ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc atcggtgtat
    43201 tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg attgttaagt
    43261 tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata tctttgattg
    43321 gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac gcacaggcat
    43381 atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc gttgctcctg
    43441 tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg atcacataac
    43501 caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg ttcaaatatt
    43561 ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact acgatgtcat
    43621 ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta aataaattat
    43681 ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa gtcagtactc
    43741 gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc ggttcatgag
    43801 gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca ttgatcgttt
    43861 tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag taatcattga
    43921 caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt tcttcgatat
    43981 tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat tctgttccga
    44041 acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa atcttcaacg
    44101 tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat tcttcgatag
    44161 tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg aagtgaaact
    44221 gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt gtcgtgtact
    44281 ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac ccaatgattg
    44341 ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc ttcacgtata
    44401 cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc atgattgcca
    44461 ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt aggaagtcct
    44521 agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc cgtatttttc
    44581 ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata ggcaaggaga
    44641 actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt cttatacctc
    44701 aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc tgaccttttt
    44761 tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa ctcgattggc
    44821 tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt atattttgct
    44881 ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg atcttcaaaa
    44941 accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt gctggctaat
    45001 tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg attcagttgg
    45061 gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca gtttgattgt
    45121 tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa gataatgggc
    45181 acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa caagatttct
    45241 atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat aaaccagcta
    45301 caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga gaaacagacg
    45361 atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc cttgcatatt
    45421 atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg cttcaaccta
    45481 taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa tttccagaat
    45541 ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat atgaagaatg
    45601 cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat aagacgtatt
    45661 atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat atggctgttc
    45721 agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg gaacaagcag
    45781 tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac gcagattaca
    45841 gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat aaactggact
    45901 tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc gtaactgatc
    45961 cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg ttattcaaaa
    46021 gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa gaattccgcc
    46081 aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg cctcagaaaa
    46141 aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat tactacggaa
    46201 aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa aaaatgatcg
    46261 ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca aaagaaaaag
    46321 ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag atcccagcat
    46381 tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt tcaaatactc
    46441 ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa ccagtggatc
    46501 gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca tttcggaatg
    46561 tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa caatcaagct
    46621 ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat gcatttgata
    46681 ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca gaagaagatc
    46741 ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt ctgccttttg
    46801 ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat gcaggcggtc
    46861 taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag gaattctttg
    46921 tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa cttttactgc
    46981 aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa aaccttgacg
    47041 aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg ccagaaaaaa
    47101 gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa taagaaggaa
    47161 tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg aagaagttac
    47221 ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag tagtaagaaa
    47281 aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac cgagaaactg
    47341 gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg agtattagag
    47401 aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgagggtca
    47461 gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat caagtaataa
    47521 gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga aggaagtgag
    47581 acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt tttacatcat
    47641 aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac tacaccgatt
    47701 aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt ttttgggagt
    47761 ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga aggttttcca
    47821 tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt gtcatttccc
    47881 gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac agattctgta
    47941 tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc tagcgtatat
    48001 tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc acttgactgt
    48061 gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac aggcgttcct
    48121 tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat gatatttcct
    48181 tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt ttgttcattt
    48241 ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga agacggtgag
    48301 gtttctccgt caaaaatagc acctacagat aatggaagac catccactct ttccttttct
    48361 gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact tccattttca
    48421 tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc gatccaatct
    48481 tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt ccaatagtag
    48541 ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg ctctctcatc
    48601 tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt gtttcctgct
    48661 gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact gctattgacg
    48721 ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa agacaaacta
    48781 ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc attaccgtta
    48841 ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt tttgatgatt
    48901 acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc atctgcatta
    48961 tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt ggccgcaaat
    49021 ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt cttgactaag
    49081 ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg gattcctgta
    49141 tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga aatctgtaat
    49201 cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca gtagtgactg
    49261 aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt gttttctgga
    49321 tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc ttttggaatc
    49381 gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg cccagcatat
    49441 gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt tcctgcatct
    49501 gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc cactgtatga
    49561 agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg atagttttta
    49621 tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt ctgataatca
    49681 aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc tcctgttgct
    49741 ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt atgatcttgg
    49801 tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg agtaggtctt
    49861 tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc ttctaccttg
    49921 atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc tgaagcgtat
    49981 aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc aggatcgtaa
    50041 taatatgcct ctaaaccagt attcgttttt ttcatctcta cgtccagttt agtaatcgag
    50101 ttcgtggccg gaaacagatt cccatgaccg tttccgaata tattataaga acctgtttct
    50161 ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac actcccagga
    50221 gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt ccccaatcca
    50281 agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc cgagtcccag
    50341 acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc ctgcccaaac
    50401 aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg ggcattcgta
    50461 taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc tttggggatt
    50521 gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc gccgttattc
    50581 gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact agcacttgtg
    50641 ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg cttttttgtc
    50701 ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc catatcttca
    50761 ctagttcttg gtatttcctc cattgttgac atcccttcaa tgttcacgtg tgcattttct
    50821 tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa aaccggggta
    50881 ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt taacaagtgt
    50941 attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt tatttttact
    51001 gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat ataacttaag
    51061 ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa aataacgtat
    51121 aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat tttgtgcttt
    51181 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggactcaaaa
    51241 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc tttgaaaata
    51301 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
    51361 catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat gtctagatct
    51421 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
    51481 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
    51541 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
    51601 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
    51661 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
    51721 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
    51781 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
    51841 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
    51901 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
    51961 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
    52021 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
    52081 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct gggttgtagc
    52141 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
    52201 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
    52261 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
    52321 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
    52381 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
    52441 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
    52501 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
    52561 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
    52621 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
    52681 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
    52741 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
    52801 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
    52861 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
    52921 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt gcaaaacctg
    52981 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
    53041 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
    53101 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca agaagcagac
    53161 gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc agttctctct
    53221 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
    53281 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
    53341 ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt tctcaaaaac
    53401 tccttattag tttaccatag ataaacctta gattttctct aaatatagaa aatccttctt
    53461 tagtccgata ctctttatag tagtccgtat gctaaaataa gtatgtaatc gaataacgaa
    53521 aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa acgaaattgg
    53581 ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag tttttctttt
    53641 gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt tggttcgcgc
    53701 aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga aactcactat
    53761 gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat gaatcaaaca
    53821 aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact taaagcaatc
    53881 gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa
    53941 ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa tctagttgat
    54001 ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca gacctatgaa
    54061 aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc acaagattat
    54121 caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt tgcgaaacag
    54181 agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt ttgttcataa
    54241 aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt ctaaagtaga
    54301 gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat ggatcataaa
    54361 ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt attgaacaac
    54421 ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat tgatcagctc
    54481 caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg ccaagaacaa
    54541 gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat cagctacgga
    54601 tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca tgtgcaagca
    54661 caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct ccaagaagcc
    54721 aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc
    54781 aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag
    54841 atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct gatgttagaa
    54901 cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat cgctgctgga
    54961 gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa aagagcagaa
    55021 gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt cctagatcgt
    55081 ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca gcaagcacca
    55141 caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca agcatctatc
    55201 gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa
    55261 aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt attgaagaaa
    55321 aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga acgtggaatc
    55381 gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat ccaagagaca
    55441 ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac
    55501 atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac ctttcgcttg
    55561 ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt ttttcttatc
    55621 tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt ccatagccat
    55681 agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc catgtcacag
    55741 tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc aatagcggaa
    55801 cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc aacttcagta
    55861 agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt tccgctaata
    55921 cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca taagtcgctt
    55981 ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt aagccatcct
    56041 tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt accgtcagta
    56101 gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct acatacctca
    56161 ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt cctgtcaaga
    56221 tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg attcctcttg
    56281 gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca tcaaatccca
    56341 agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca aaaggcatga
    56401 atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac aacgcgatga
    56461 taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg gcttcaagcg
    56521 acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg tatgtaccat
    56581 tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa
    56641 aaccttgcat atagccagac catccggaag caacagagga aaccgctagt aaaaattccc
    56701 cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca tacagatagg
    56761 catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt agtccagaca
    56821 aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca gcattttgat
    56881 tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca attccaagca
    56941 tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg agattgatct
    57001 ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa aacagcgatt
    57061 aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg caaccaacaa
    57121 taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc ccacctaaag
    57181 tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca agtgatttga
    57241 ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc atgtttttcc
    57301 acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct aagatcattc
    57361 cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg acgatgatgg
    57421 tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta ccaaaatcaa
    57481 gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc aaatacgcac
    57541 cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt tgtgtccatt
    57601 catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca gtagacaatg
    57661 gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt tgtccaacac
    57721 tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt acaggactaa
    57781 caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct agtgtttgtt
    57841 gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat gccaacatag
    57901 ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg ataagaaact
    57961 tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat tttctaacgt
    58021 ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc ttttccttca
    58081 gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc taaataaagt
    58141 cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa tcccttatca
    58201 aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac tgaaaggctg
    58261 actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg cgtatatttg
    58321 atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc ggtcaaaaca
    58381 gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata atttgcctga
    58441 ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag gtagtcccgc
    58501 gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc gatttttgat
    58561 aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat cgaacgaact
    58621 agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc aatataatct
    58681 ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc acggatcagc
    58741 tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact caaataattt
    58801 tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct cttgctcaag
    58861 taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa ataagcaatt
    58921 gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt caatgcaata
    58981 aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa aaaattcatc
    59041 ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt tcgatatatc
    59101 cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact gtcaatgtat
    59161 tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc cgacttaaga
    59221 tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg ctcaaatcaa
    59281 tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg ccattgtggc
    59341 tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat gcattgattt
    59401 ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc cccatgttca
    59461 tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc ggtactttcg
    59521 atacttcacg tattttttga caccaataat agccatccaa aacgggaaga ttaatatcta
    59581 gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg aaatcggtcg
    59641 ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt cgaatgatct
    59701 cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt cttcttgcta
    59761 ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat gtcattttag
    59821 caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt ccaagcagct
    59881 taaatttatt atgttattct taataattgg tctattatct gttatttttc tttcgttgtt
    59941 tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact agcaatatac
    60001 ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct atctttgaag
    60061 agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga gtgttcacgg
    60121 tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt gctaatgtat
    60181 ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc atccgaccat
    60241 acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta aagagttttt
    60301 tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct ccagttggaa
    60361 aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc gtttcatcaa
    60421 ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata tgttgttcgt
    60481 aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga tctttgactt
    60541 gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt tcatgcactg
    60601 ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga taattctcga
    60661 tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat gtaaaaactg
    60721 cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg atttgggttt
    60781 gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa aaaactgcag
    60841 caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc agacttcatt
    60901 ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat tagtaagaat
    60961 gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt cggtactctt
    61021 tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct tccataagag
    61081 atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc tggcgccatg
    61141 gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta tccatttcca
    61201 tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc tgctcctgat
    61261 tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga acggtcttca
    61321 ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc atatttttca
    61381 aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc ataatcaaat
    61441 tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc cgcttctagt
    61501 tctgtggaaa ccttcaaatt atatgtgatc gttaaatctc ctccacgtgc atctgctaat
    61561 ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt cacgacaaac
    61621 ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt ctcgccggct
    61681 cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc tgtgattttg
    61741 taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata aggaataggt
    61801 gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga aatcgtttct
    61861 tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc tacattcatc
    61921 tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt attgtatgtg
    61981 tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat cactggcatc
    62041 ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt ttctaagaaa
    62101 agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt agagaatacc
    62161 gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata ttttaaaaac
    62221 caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt aagaccgtct
    62281 tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc cgttacgtca
    62341 aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga aaccaatccg
    62401 ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt aatcgtcact
    62461 tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc gaaaatccct
    62521 attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt tttattcatt
    62581 tttttctccc cgttcttttt ttattaataa aacagcacct aacaataaaa gaattcctag
    62641 taaagaaatc aacgttcttg cttcattcgt ttgcgggaat acggaccatt ttcctgattg
    62701 acctggattg cttggaccac tcggctcttt cggtttgtcc gttgtttctt ttacggtctc
    62761 gttataaaca cgtggtgttg cggattcata ggcagagact ggaactttcc cggattctaa
    62821 ttcagccatc tcctgtccgt taactactac atattgtggt tggccttttg catccaccca
    62881 aaattcaggg atgatgacct cgattctctt acttgcttca tccatctcgt aaccttccac
    62941 accttttaat tcttcaaaat aataggtgcc agaaggcaag aaacgttgtc ctgtcgtaac
    63001 aaggccctct ttatccgaaa tgaatttgct tacatttttg tcattcaaag gatcattgtc
    63061 tgctggtttg atccattgat ttttcaagtc attagcagga gccatatcta aataatattt
    63121 ccccccttct atcatctgat atagagcaaa aacagcacct tctaatggtt ttcctttttc
    63181 agttgttcct ctttgacgtc catacttgaa gaagtatgga tctcttagat aaccgacatt
    63241 tttaggatag atatgaatct cttgcagttc ctcggttgat tctgtcgggt gataaattgg
    63301 caaaatagca gcaattggga tagctgtctg atcgatatca acatccaatc caacttctgg
    63361 atcaatcgat tcttcgaaaa tcaaataaac tgcatcccga tcttggtttt tcttaggtgc
    63421 tttgatacgt gcaatcccgt ctcctaatcc gccaaccgta tctttcccag ttgttaccga
    63481 agtccctatt tggacaagcc tttcttctgt gattagctga cggatattgt ttctgttcgt
    63541 ttcagcgatt tgctggacga attctttagg agtcatgttt tcagcagctt ttttttcatg
    63601 ataataatcg gtcatgtcgt aaatggcaaa tgtcgctcca tttagtggca ccgtctgtga
    63661 aatgatatct gctgtttccc ctgcttcctc agagatagcc aagccatcat tttcatggta
    63721 ttcaaattga tcgttataat cgatatctcg aatcatccgt ttatgcaaca cgaattccac
    63781 gacatcttcc cctgattctt ctgccgacat cgcatgtgga agaaggactg ctgcaagcgg
    63841 tagtaaaaca agtaattgaa gcatcaaaca caacagcttt ttcattacct tgctccctcc
    63901 tttttagatt gtctgacaaa caaaccatag gctgctcctg caagcgccat gaagaatgca
    63961 gcaagcagga tatagaataa tcgtccggat cctcctgtag caggcaacgg attttcagga
    64021 tcgttcccga cactaagctg taatctgatt cgatttgtct ccaaaagttc atattctaac
    64081 tcttgctgta gatcaggcgt ctctgcttct tcgtttggat aatacgaaat ctcaactttt
    64141 agtgtacccg cttcttttcc tggaactacc cgatcatttt ctatgtcagg ttcttctcga
    64201 taactaattt ctaaaataaa gtaacctgga agttttctat agctgtcagg tgacttcact
    64261 tccttgatcc gatagacccc ttccaacaag tctgaagcat tgaataggga agaatcgttt
    64321 ggatctggtg ccaagcctcc ctcgatgatt tcatactctc ctgcactatt ttgtttctca
    64381 attgaaaatt ctgcgccttc aagacgtcgg ctattcatac tgtcaatttt caaaagttcc
    64441 aaatcgattg gcgctagttc atcttctaag gttacttgta gtacaccatc gatgatttca
    64501 tagccttccg gcaagggagc agtttcgtct tcgatgaccg tatcttcaat gataaactta
    64561 ccttcttcat tcagtgcaaa ctggaacact tgatcagata gccgataccc atttggtgcg
    64621 acagattccg agacctcata ttcaccaata gacagatgca gaggttggtc gtaatttaaa
    64681 tcgacatact tttctactcc tacggcacga gaatagaagt atccgatccc aggcaaacgt
    64741 ttcgattctt ctggttctaa cggattacca gtgtatgctc gatacagcgg gaaggaagaa
    64801 tcccccactt tcctgatatc gaaacctgca ccatctaatg tatggccttc atggtctaat
    64861 tttctaactc ggatctcccg ataatcaaac gtattgtaag cagtgaatgc tacccataaa
    64921 cgatcctctt catctgttgt atacgtgaga tattgccaat tttcttcacc tgtttgcttg
    64981 agttggatag tccctatagc attatcgtaa agtcgcaaga tccatgtgtc attttcatca
    65041 ggttctttga aaccttctgg gttctggctc tcaatgattt tgtattctgc atagttggta
    65101 gtttcttcac tatccttata aacaaatgaa tctggctctc cgattggatg aataactttc
    65161 tcaccatcca cgattacata tttcccgtct tcatctactg aatagaacgt gagtctccct
    65221 ttctcttcct ctacttcgtt cacaccagaa agccctttcg ctactggata tccgatttcc
    65281 agtccgttac catctgtttc ataaagttca aactctgcgc cattgattgg catattactg
    65341 ccttcaatta gtttattgat attcatgtgg attggcttga attcatttcg catttccagt
    65401 ttgacataca actcatcata ctctgtgcct tctatgttag gctcttgtac aacagaaagc
    65461 ttgtgatatt ctccttcata ataggaattc ggatctttac cttccggtcc gccgactctc
    65521 gaaccagatg tatattcatc tacaagctcg tagtgaccta acatctcatc ttcgttggct
    65581 gcatattttt tgatttggaa ttgatacgtc tcatctacta actgataacg tggtggtcca
    65641 gcgatctctc tgatccaaaa tatgtcgctg actgctccag tttcatctcc taaaatcggc
    65701 atcttcgttt caaagatcgc tattccgtcc gtggtagttt tacttgtttc gattggttcg
    65761 gcttccccac tctctggatt ttggtcatat aatgcaaatt cgactccagc caacggctcg
    65821 ccattttgac ccttcttgct gacttggaaa ttacggcctt tatgttcgta acgaatggaa
    65881 gaagatggga aatacatccg tccagtagtg atatcattct cataatcctc caggttctga
    65941 atataggcca tattgttcac tgtgcaaaaa tttccgtctt gagctcccgc atcaagtttt
    66001 acttggtact tgatcctgac catttcccct tcacctaaag aaatcggaga aaatttataa
    66061 gcttggtttt cccgtccgac atacgttgtc ggagtcgcca ctacttctgg tggggcttgt
    66121 ccatgacgtt cttcatccca tatttctggc tcttggtact tcatgccgtt ttctcctgga
    66181 tctagatggt aaaactgaat atctgccgat ctgccagctc caaatggtga gacattaaaa
    66241 ccagtttcat cataaagtgt ggcttgctgg atcgttctct tgaaggtaga agagatatcc
    66301 tccaaggctg taacaatgtc attcttgttg ttcgcattgt aatatcgcgg tgttacgcca
    66361 tcttcttcta aatcagcaat atttttcaat acatttcgtc caatagcatc tagtcgttgt
    66421 ctcccgctaa cgtttccatc gattcctaaa ccaatggaga agatacttgg cgggttctct
    66481 tgatacttgt ttttcagcca atgatgataa gcaactgtgt tgatcgcaga tgacttttct
    66541 tgcgaagtag gactgccatt cgtattgtta ttagaagaac catcaccaac ccagtgagtc
    66601 gctttattat ctccttcttt tacctctcca taacgatagt gtacatttac ttcatcttct
    66661 ggacggttaa agtcagatgc ataggtaact ggatgagtaa accctgcatt agaagtattc
    66721 ccagaaaact cctcaaaatt tctgaacaaa acgtcattat cttcagcaat cttatcttcc
    66781 atcacagtcc agttgctcca agctccatta gtgctcgtac gataacttga ctgaatcggt
    66841 gcataactga aggtcggtgt cccatctccc acaacgatta ggattttttc tggatttcta
    66901 ttttctccgc cattgtcttt atacaatgtt tcatagccat ttttaagccc tagtgtaagc
    66961 ggagttcctc cgattggtgt acgatttaat gctgtattct cacttcccgt caaacgattg
    67021 atatcatttg tcaatggaaa gatgtccgaa ctgatttgct catggttatt gatagctgta
    67081 ctattatggt aaaaattcac cataccgatt cgaatatcca gactcgggtc agagagcaga
    67141 tctttactca tttcgtttac tgcctcaatc aacgcagcat ttttagtttg actattggtc
    67201 ccagcggtta attcactcat actcgctgac ttatctagaa caaaaacgat atcgattgga
    67261 ctgatttcct ttttctcgcc cccgatgaca tctagataga cattgtaaag ggttgttgca
    67321 tcttcaggat gtagatcaga tgctgtgctg cttggcagaa caggttctac ccattttttg
    67381 atgtacgccc cgttataatc caagtaactg ttttcaaaat tccgttcatt tccccaaagc
    67441 tgaacggttt gagctggaac gtttctatct tcttcgcctg cagttgatcc attattagta
    67501 gcatagtcgt aatttcgcca gttcgctccc ccgtttgtat aatcactatc atcgatcaag
    67561 ttctctccgt catatggcga gcctttgtct ccttgatagc gttcattgct ttgtgtccaa
    67621 tttcctaaca cttttgtata gtcattcgtt gtaaacttca tctattcttt catcaaccac
    67681 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    67741 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    67801 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    67861 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    67921 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    67981 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    68041 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    68101 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    68161 atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta
    68221 attataattt aatttaattt taattttaaa tcaaatgata tcctttttag ttaaaaaaca
    68281 ccaattatac aacatttatt attaaaatta aattataaaa ttttataacg ttctcattta
    68341 acaaaactta agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa
    68401 ataaagcaca aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac
    68461 ggcagcgcat aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg
    68521 ataatgtaaa agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa
    68581 tattcctgca actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt
    68641 atgtcctgaa ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt
    68701 tttttcataa ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa
    68761 atagatcaaa ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac
    68821 taaaacagca gcaaggagca accaaataaa accaccatta gagatattac tgataaattt
    68881 caaaaaattg atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact
    68941 atcatcgatt ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt
    69001 gcgtacgatg gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag
    69061 caaaatagat tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat
    69121 agaaaagcgc gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt
    69181 ctttttgttc cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca
    69241 aaaacaacgt agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct
    69301 gataatcgat caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa
    69361 aagagaagtt ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt
    69421 ttttgtaatg ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag
    69481 gcaagacgca caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga
    69541 tttctaagaa ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc
    69601 tcctgtcaaa tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt
    69661 cgggatcaag acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt
    69721 ctgaaaaact tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag
    69781 aggacctaag aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc
    69841 aggtttcgtc aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat
    69901 ttcagcagct tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat
    69961 attgaacgca ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc
    70021 gatcttggag aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt
    70081 agaaagaacc aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata
    70141 agcaataaaa gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt
    70201 atttcttgca tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac
    70261 gattttttct gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga
    70321 agtaaacacc atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac
    70381 ataacttagt attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat
    70441 ttgccgtcac ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc
    70501 agaaccaaca cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa
    70561 gctacgaacg ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg
    70621 cctgttccat ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta
    70681 gaggtgatca catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac
    70741 tttccccaaa cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt
    70801 tgtaatgtag ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc
    70861 aacgcaggtt tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag
    70921 cccagaaatg tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag
    70981 actgctactc ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct
    71041 ttgatttttt gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca
    71101 ttgaagatga cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg
    71161 aacattcttt tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg
    71221 gtgaaactac caataactga aaaaagcata ggccctagca taaataccaa gaacccgata
    71281 ataaaaggcg agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa
    71341 cgcttcaaat tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg
    71401 cttgactaag ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt
    71461 tcctaaaaga atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa
    71521 aggaactggt gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt
    71581 tcctttatcc gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat
    71641 gatatcaaaa cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat
    71701 gaattcataa gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc
    71761 accccacgac caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc
    71821 aaaatcatcc ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat
    71881 ggctactaat ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt
    71941 attgatcgta tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac
    72001 tttattttta gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc
    72061 tgcgtttagc gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt
    72121 aaattctttt ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt
    72181 tgtcggtaga tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat
    72241 gacttgtgta tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc
    72301 ccaaagctgc ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt
    72361 tgcttgctgt gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg
    72421 tgcatttcca gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata
    72481 cttataattt acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat
    72541 agcttctttt cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc
    72601 acttgttttt tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc
    72661 gcatcctcct aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt
    72721 catattttac ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt
    72781 tgttctgaaa ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata
    72841 ctttatataa ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa
    72901 tgtattaaaa atagattagg aggaattttg atgtttagac accagaaaga attgcagttt
    72961 gaagcaaaac cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc
    73021 ggccaatatg gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga
    73081 ggagaagaaa aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg
    73141 gaaatgttgg cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa
    73201 gcagcgatgc aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac
    73261 gcgatcgtag ctggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt
    73321 gcctatatca ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa
    73381 tcacaaggaa gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa
    73441 gatatgctgt ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca
    73501 gtgaaagaat tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac
    73561 aaagaacgaa aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt
    73621 gaaggttctt gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa
    73681 gaacctaaga tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg
    73741 acacctccat tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg
    73801 ctttgtgagc aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt
    73861 ctctagagct ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt
    73921 atgttcaagc catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac
    73981 tccttagccc ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc
    74041 atgtttttct tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt
    74101 cacatagtat ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca
    74161 atctcttgat gatctcctga atattggctc gatattgatt ataaattatt tttcttctat
    74221 actttggtgt gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc
    74281 cctttattgc tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa
    74341 ttaggtctaa agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt
    74401 tcagaaaaaa atgaaatgaa aaagatctct tacggcaatc tattttattt ttttgatttt
    74461 ttataaatgg aaaatgcttc aagaagtcca agaaataaag ccgtataaaa acaagaacct
    74521 atgaaatctt tatttattag atattcaaca gaaatcccga taacactagc caacaatata
    74581 tttatcagaa gtgtaaaata aggatgcttt ttatagaata gaactagtgt tgttttcata
    74641 gactctcctt tcttcatatt tccaattaat cacaaattca cggcattact agaaaaatta
    74701 aaggggcgca gaagtggaaa aatactcttc gactttccct acaaaccagg ccatcttgtg
    74761 tttaataact ataccaaatc taaggtacct ctaaatctaa attccatttg tggcggtgac
    74821 ctccaatttt taactcttct atctcccatc cagtatacat aaccttgata cttcgaccac
    74881 ttatatatcg aacacacaaa tacctatcca ttgcaggagt actacctaac ttagttgcta
    74941 cttgcatcta aataacagct gaaaagatag gacttactct tagtaaagta tctgaactat
    75001 taatttttaa actatcattt ttatcctcaa ctacatgcac atctgttgca acttcacttt
    75061 ctgtctcatc cagataaatg attaaggaat cgggaattgc aatatcttca gctgacgctg
    75121 gaatagttga gctaaattag tcatcatatc tttattataa aataaccaac agcaaaaaat
    75181 acaatgaaaa acaaacttgg aactaatgta tcattaataa atgttgaaaa attcagcaac
    75241 tcattactgt atgattttag cagcccaacc aacatatacc ctataaaagc taataagatg
    75301 gttaggaatc ctttagaatt tttactcatt catagacact tccttaaata acttttatta
    75361 tagaaggtat tgtaaataat aatattgaca aaaagtgact caaaataaaa aaaaacttac
    75421 attatactgg aatgaactgt attgatagtg tacctaaaag ctctactgac ataagatcgt
    75481 gtaatcggta aagtaatttt attaattata cgggatgtaa tcaattagag aataaactaa
    75541 tcataaaagg tacttttaca ttcataattg tcgctttgag attctacttt ggttcactat
    75601 ctgacccaat tgcacatttt cgatcattac aagtattggt gtcgttagaa caactaaata
    75661 aaaactagcg gtagattttt cagaatctat gctaatttct gtatggatgt tccagtgatg
    75721 gcaaatttct cttttcattg tttttcttgt cgcatgatat gcctttttgt gttattcagt
    75781 taaactatag aaattctttc ctagacagat cttgatccca aattttacgt ttttgagcat
    75841 cgatatagtt taaaatgaca ttactcatat ctaaagagtt tagatttttt aggactaaaa
    75901 ctgtcagttg ttctttttct tctttctgtt aaagtattgt cgcgactttt cgtatacgtt
    75961 aagatatgtc gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact tgaggaaatc
    76021 acctcaatca ttcgtttaaa atgctatata ttctttagtt taagatacgg aaattaatgc
    76081 catcgaccac actagtagaa tgaacgacat ttacttggcc tttttttaat ttcgttatgt
    76141 ttagcatata aaaatattat agagtttact tacaatgctt aaaacatata ctgtataatc
    76201 tgatcaattt tagccatgtc ttgcaatggt aagttttcta tttttttgag ttttcgttcc
    76261 ttaaaatcaa gagattttaa ttgtgtaata attacttgtc cttttatatc tagctcttca
    76321 ggtaatgagt aacgtgtcgg taaattcttt atagtggaag taattggaca tatgaccgaa
    76381 aacattgttt tacggttaaa ttcataacga gagacgacta gtcctggtcg tcttttttga
    76441 atttcttttc ctacggaggg atcaaaatca atccatacaa tatcaccttt tttaggaata
    76501 taaattcttt cttctgacac tataaaattt ctctcccttc aggagttaaa tcttcccatt
    76561 cgtctttctc ataatactcg gcctcttccc caccactaaa tggatcttca atttttggaa
    76621 ctaacgtaat tgttccatca tcagaataca caacgatata ttcttggttt tctgaaggtt
    76681 tctttccgtt atcagaaggt aaagttacca ctacagaact tccttgtaat ctagattttg
    76741 taacaagcat tatcatcact cccttaataa aagtatacta tataacacta agtaacgcaa
    76801 aattcccctt tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat
    76861 aaatagaaat aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa
    76921 cattgaacct atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca
    76981 attttgcaga taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc
    77041 ccgattataa acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt
    77101 tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa
    77161 tagaaacatt ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga
    77221 tcgtagctat ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata
    77281 aatggcaacc tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt
    77341 tgaattttag attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata
    77401 aaaaagactc gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg
    77461 tgaccctata gatgcagtat atgcagctta tgagcatttc ttctgttctc tttgtttttg
    77521 ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt
    77581 tataccttct ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa
    77641 atatctgtta cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg
    77701 attggtgcga ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca
    77761 tcctgatggc tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata
    77821 aatttgtctt tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt
    77881 gatgaaaggt ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt
    77941 ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt
    78001 ttcttaacgt cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct
    78061 gcttcggttg tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat
    78121 caaaaaaata acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc
    78181 aacaagaagt tcgcaccaac aaaacacttt caatttttgt agaactaaaa aagttgtgac
    78241 aagtgacaaa ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa
    78301 tattcaacaa acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg
    78361 gcaggcttac gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa
    78421 atttatgcaa aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag
    78481 cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    78541 cccaaccaaa cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc
    78601 agggcagctt tttcacaacc tctccactaa acgatgttca atcagctaac cttcggtatt
    78661 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
    78721 tcaaagcgct gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa
    78781 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    78841 gaagctgacc acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa
    78901 tctttctttg accaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa
    78961 caataagata cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc
    79021 atcggacagt ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg
    79081 attgactaat tctagtagca acagacggta ttcttcatcc actgtactca actcaataat
    79141 caaattacct acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca
    79201 gtccaacatt tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa
    79261 caattctgtc tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt
    79321 tgaagaaaag taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg
    79381 gattgacgtt tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt
    79441 ttcctttgtt ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta
    79501 aattcatcat tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag
    79561 ccctcactat aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga
    79621 caaaagcaat tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga
    79681 tctgcaataa agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca
    79741 attgcatctg tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa
    79801 tcgctacaca agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc
    79861 aattggagcg ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc
    79921 gtattttcat ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc
    79981 actgattcat attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct
    80041 gcaccgccaa attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt
    80101 ttgtatcctt tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat
    80161 ttttctaaga tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact
    80221 tgcgggtcag tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc
    80281 gttatttatt ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga
    80341 tctctcaata acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact
    80401 tcatgagaat cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact
    80461 aaaactttga atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc
    80521 atatacttga ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct
    80581 acattggata aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt
    80641 gttcgtttat tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata
    80701 taagtgaact gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta
    80761 cgaaggtatt catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct
    80821 aatccaagtg atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg
    80881 attgttactt cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct
    80941 acttgatcta cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa
    81001 atcatttgcg cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg
    81061 acaactttag atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc
    81121 ccgatgatca ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc
    81181 aagtagcgac ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg
    81241 atgaaaatcc gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga
    81301 tggttagaaa gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc
    81361 atatgataat ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct
    81421 gtaaagtcga ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg
    81481 ttgatacatg ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct
    81541 tttgaagcta acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt
    81601 tgatattttt ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata
    81661 tgataaccga taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga
    81721 caaaaagcgg tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat
    81781 cgttacttct acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc
    81841 atcttctgca attttttggg cttcttcttc gctgatgcca tcgatatgca cttcgaatac
    81901 tacaccgatc tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt
    81961 ggactctgct gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga
    82021 agcataacct gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg
    82081 agtgattttc atatgcatcg agccatttgg ttcctcgact tctccattgc gcccgccatg
    82141 gttgatcatt gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt
    82201 gctacttggc tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt
    82261 attcgaatac cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg
    82321 tgaacaacag cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta
    82381 tatgattgat tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta
    82441 aaaaaataac catattcgtt accatttttt cctccttttg ctggtctccc taccagcact
    82501 cgatttaacc ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt
    82561 tattctgctg ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact
    82621 aacgccaact tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca
    82681 tgtaagctta aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga
    82741 ataccttctt cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc
    82801 atttcttcta atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg
    82861 atttcttgta cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct
    82921 tttcttttta attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc
    82981 agaaaatagt tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct
    83041 ttcccaagac ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa
    83101 cgaacttgga tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa
    83161 ttcgtcacga ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc
    83221 aaattatttc gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct
    83281 gtatcgaaaa tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc
    83341 atcgcttctt tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa
    83401 aattgggaac ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca
    83461 ccagcaccat ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt
    83521 gttccaatcc cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct
    83581 gctttttcta cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa
    83641 gcaatatctt ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa
    83701 tagggaagat ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga
    83761 gtggacataa acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt
    83821 tctttcagac tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga
    83881 ccaatcccaa tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag
    83941 tttgctagtc cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct
    84001 tcaactggta atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc
    84061 atataaaagt cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg
    84121 cttcttcttc tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa
    84181 cttcagggta agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag
    84241 tcaatacgat aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg
    84301 tacgcaaacg ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta
    84361 ttttgttttc attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg
    84421 cttgagtcac atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa
    84481 taggttgttc tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag
    84541 acacctctca atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt
    84601 tttactttta ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa
    84661 atcgtcttca ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt
    84721 tctttagaag ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt
    84781 gctggttctt cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc
    84841 tttttgaata ctacttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct
    84901 tcttgtgcta tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa
    84961 tgttcttttt tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc
    85021 agtggataga cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg
    85081 cgtattgcat agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc
    85141 gggtcgccga tccctctgat gctaaagaat gattttttag gaacctctag tatttgcggg
    85201 ctttttttca tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct
    85261 ccgcgaaatt ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg
    85321 tgttgacact atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa
    85381 cacgtttcgg cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt
    85441 tttttacgaa attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta
    85501 cctgcagtaa ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg
    85561 aatgtaactt tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg
    85621 gtaacaacga tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat
    85681 cgcaatttta gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata
    85741 atgatcgatt tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt
    85801 gcagcgacag gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc
    85861 aatcgacgag gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt
    85921 ggccccactt atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt
    85981 cttgtgcccg ttttgattct ttctttgatt atcggctggt ttgctattcc cgagatggca
    86041 gtacaaaaaa gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt
    86101 ggaagtctaa catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc
    86161 ggcggtattg gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc
    86221 cgactgtcta ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat
    86281 cagtttttat tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga
    86341 agtagtcctt ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt
    86401 tctccttttt ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg
    86461 ctgattcttt ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg
    86521 ggtgtattga ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat
    86581 atgatgacca ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg
    86641 ttcaatacct tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc
    86701 aattacgtcc agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa
    86761 gcagcattag gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat
    86821 ctgttctttg ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt
    86881 ttgtgtgaaa atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg
    86941 gtggcgggat cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg
    87001 ctttatttga tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt
    87061 ggttgtcgaa acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt
    87121 tccctaaact tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac
    87181 ttttgcttcg acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag
    87241 aagaagcgcc agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac
    87301 ttccgttatt gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa
    87361 aagcatattt agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg
    87421 caaaagtcac tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat
    87481 acacagctga tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat
    87541 taggctatgc tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt
    87601 ataagatgag tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt
    87661 tcatggatgg aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg
    87721 ctcctactag cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc
    87781 cagatcagat aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag
    87841 atttttcccc atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc
    87901 taggctcttc tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa
    87961 atactggtca ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa
    88021 atccatcata gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt
    88081 acttcttgaa aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt
    88141 ttgcttcatc tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat
    88201 caagtcgttg tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg
    88261 gtataggtaa atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt
    88321 gcacgattct ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat
    88381 atccagtatt caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt
    88441 gatagtcttc cctaagagga tagacacgga aaggattagc aaatggttca atgactagct
    88501 catctagctg tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg
    88561 caccaaaaga cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat
    88621 ctttcatgat ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg
    88681 tcgcataacg tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga
    88741 ctttttctcc tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa
    88801 tggccggatc atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag
    88861 tagcaaaacc attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt
    88921 gaccttccgt atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata
    88981 cagccatcgt ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa
    89041 taaagccatt gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc
    89101 caaagtaacg taatcctagc acaaccgctg tattatggag agcatcaaac aaaatgagtc
    89161 catttggaaa tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat
    89221 caaaggaaac agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca
    89281 gcaaataaga atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag
    89341 aaaattcttc agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt
    89401 gatacacagc ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa
    89461 agatccgccg agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc
    89521 taacatcagc tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg
    89581 ttcctggact cccttttgct agttgataag ctgtcttcct atcaaaaact tcatgaacat
    89641 tattaccgta aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac
    89701 ttgtgatttc gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa
    89761 acaaaaaaac gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt
    89821 gggaagcaaa cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct
    89881 cggtttcatc attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt
    89941 agcagcatcc catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt
    90001 ggcagctgaa tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg
    90061 tccgttagta gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga
    90121 gcttgaagtt ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac
    90181 aggtgcaacg tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt
    90241 tgttttgttt tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc
    90301 aacataaatg cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga
    90361 gttgtctcct gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata
    90421 aacttcatca gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa
    90481 agttgtacct aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt
    90541 ttttattctt tcgacgctat taactttatc atttagaaat atttcatagg taaaagaaac
    90601 atgacattta atgacaaaat aacgaattat ttaatatttg taacatttaa acaaatattt
    90661 ttttgataca aaaccataaa agccttgttg tataagcaaa gtaatcgttt ttctatgttt
    90721 tttttatttt tgaatgaaaa acgcagattc tgtaacattc actgcaataa tccataaaaa
    90781 aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa aaatacaaaa
    90841 aaaccagtaa ttgttacaac aattactgga caaaaacttt gaataatgtt aactattttt
    90901 ttattttaac tttctccgca ataatttctg ggcatgttcc aattcgttta taacgacttc
    90961 ttctgtttct ttagctaata tttcatctaa tacatcaagc catttttccg gttcttttgt
    91021 tcctagacgt ccaatagccc aagctgctgt tcctctgatg acagggcgga catctgttaa
    91081 acattctgaa atctcaggca atgccgttcg atcacctaga ttagctaaag caatcaatgc
    91141 gtttctctgc aaaggtttct tcccacgcca tgaaccagct aaatgcccaa attgctgttt
    91201 gaagtcttta ttagacatcg aaagcattgg ctttaatttt ggatatattt cgtctacttt
    91261 tggttccatc tcttcatgaa aatgaaaatc ttttccttga ttgtaagggc agactaactg
    91321 gcagatatca catccataaa tgacatttcg cattttttta cgatattctt ccggcatcat
    91381 cccttttgtc tgagtttgat aagacaaaca tcgctgggca ttcattcttc cgtcaccaag
    91441 caatgctttt gtcggacaag cagtcacgca gcgcatacag tcaccgcaac caaattctcc
    91501 tggtttatca ggctcaaata caatattagt aacgatctca ccaagataga caaatgaacc
    91561 gaactcttca gtgatcaaca atccgtttcg tccaataaat cctaaaccag ctcgctgtgc
    91621 tacagcgaca tcgatcagtt ctccagtatc tacttgcgga gctagacgcc attcctcttc
    91681 tttttgccat ttttctgctc ttgtttggat gaattcgatc aatcggttca aacggtctct
    91741 gagtatatca tggtaatcga tcccccaaga agcacgagca aactgcccgc gtttttcatc
    91801 cctaggcact tcttcatgga tcctagtagg atacgcaagt gcaatggaaa tgatcgattt
    91861 cggctgatca aatgtcattt ctggataaag acgctcatcg atatttggat gttcaaagcc
    91921 agaagtatgg ccagctgctt tttgttcttc caacgattct tttaatgaat caaaaggttc
    91981 cgccgtggtg aacccgattt tgtcaattcc taatcgtttg ctttcttgta tgatttcttc
    92041 ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg tataacccat aatatagttt
    92101 ttcttttttc agctggacaa actggatctg ctgatcggca ttcttctttt cttccatcca
    92161 tttttttctt gcagcaaata cttcccaata atttgttgaa attaattttc ttttgttatc
    92221 taatgcatta aaaaaattca atgaaactgg ttttgtactt aacagttcat ggagccttac
    92281 ctcttcccat ggcgaaaact cagacagatc gcgggaatac aatacttctt catagagatt
    92341 tttttgctgt gtgtacgtga atgtttgcgg gattttccca ggcctagcaa tttctactag
    92401 acgattataa aacatattca ttaatttgct gttggcaata aattgacttt tataataggc
    92461 cgactggatc tttttgatta ctccttctgc tttgatgact gaaaataatg caggccatgc
    92521 tttgacggtt atttcgtcgt tttgtttaac taatacgcca ggtgtgaagc cgactggaga
    92581 aaggaacaca aaataaccga taccggtatt ttcatctaaa ggctgaaatt catcaaacaa
    92641 ccatttgcct atgtacagct cttcatctgg ttctgttgtc tcattttgat ttgctatgaa
    92701 taatttagct aattctgctt caagaaattg aaggtcttga tccttgtcgg tattttcctg
    92761 ctggctgatc aatcgaaaga cttcttcttt aatcgtatat ttatctttac gtgtaaaatt
    92821 catagccaca ccttccttcc ttactctaac tataacggaa tttcttcaaa aaaggtatct
    92881 gtacgacgaa aaacagcata attttttttc atatctctta taaaaaatgc aaggtctgct
    92941 cctcaagcta gaccttgcac ttttcagctt tcttttgttt gtgacaaacc ttcatggatc
    93001 gtttcaatat tccatttcat catctgatag taagaatctc cttgttctcc ttcttttgct
    93061 atggaatcag tgaacagttt atcatagatt ttcaaacccg tttcttttgc tacccgttcc
    93121 atgcttcttg agtctacact ggtttccacg aataaaaccg gtacttcttt tgctcttatc
    93181 tgatcaatga tcgctttcat ttgatcaggc gtaccttgac tttctgtatt gatctcccat
    93241 atataagctg ctggcaaatc atatgctcgt gaaaaatact tgaatgcccc ttcacttgtt
    93301 actaataatt ttttgttctc ttcgatagaa gcaaaacttt ccttcgcttg tgtatctaat
    93361 tcttttagtt tttccacata tgcttctgca ttttttttat agagtgtcgc attttctgca
    93421 tctttttcag agaatatacg tgcgatttcc tccacatatt ttattccgtt agataggtct
    93481 agccaagcat ggggatctgc ttttgtatgt tcttcaccgc tagttaaata aagaggttct
    93541 acatttttgc taactgcaaa ataatctttt ccttcttctt ttttagccgt ttccatcaag
    93601 ttatcgaacc agctgttacc tgtttcaaga ttcaaaccgt tgtataaaat aacatctgca
    93661 tcgcttgcct ttttgatgtc ttctggtagt acttcatatt catgcggatc tgttccgact
    93721 ggtacgatac tgtggatatc tatttgatct gttccgacct cttttgccat gtccgccaag
    93781 atcgaattgg tagccacgac tgacaacttt tctttacttc cttcatccgc ttggtcagaa
    93841 gcctgctttc cgcaaccggc taataccaag aaactaactg ccaagattaa aaagagtgat
    93901 ttttttgttt tcatctatct gcttcctttc tgaaataaaa ttccttttgt tggagaaaat
    93961 aaaaaagcaa ggataaaaaa tgcggtacat gctaacacga tcgtagctcc tgatggccaa
    94021 ttgtaactat aactgaaaaa cacaccgaca actgcactta gcataccgat tcctgcagct
    94081 gtaatgatca ttttcagtaa atggttcgtt agcaagtaag ccgtggcggc tggtgtgatc
    94141 aacatggcaa tcaccaagat tgttcctact gtctgtaaac tgactacagc cactaatgtc
    94201 aaaaagaaca tcaaaagata atggatcagc gatgtgttca gcccataagc ttttgccatt
    94261 gtcggatcga atgatgtgat cttcaattct ttataaaaga aaaagacaaa tatcaagacg
    94321 attccactaa cgagtgctgt cagcaacaaa tcgctttccc taacagctaa gacattccca
    94381 aataaaatat gatacaaatc tgtcgcactc tgggcatatg aaatcaagat aatccctaat
    94441 gcaaaaaagg agctaaagac aattccaatc gctgtatcat ttttcaatcg gctccgctga
    94501 gtcacgaaac caatcactgc tgctgttagc atcccaaaag cagtggctcc aaagatgtaa
    94561 ctaaaaccaa acatataaga aatggctaca cctggaagaa ctgcgtgaga gatagcgtct
    94621 cccatcaaag acatccctcg taaaataata aaagaaccga tgactccaga agcaagtcct
    94681 acaagcatgg aagtgaccaa agcgtattgc aggaattgat aattcattaa tccatcaata
    94741 aaggattgga tcattttttc tctcctcctt ttactaaatg tctgatgatc tcaccatagg
    94801 ccaattgaag gttcttcgat gtaaagactt cttgtactgg accggaagcg atcagctgtt
    94861 tatttaaaag aataacctca tcgaaatatt cttctacttt atgaagatca tgatggacga
    94921 tcaaaatcgt ttttcctgaa ttcttcaatt cctgcaagat gtcaaagatc tttcgttcac
    94981 ttaacgcatc aatccctacg aatggttcat ctaaaaagat ccattctgct ccttgggcta
    95041 gagctcttgc aataaaaact ctctgaagct gtccacccga tagttcgctg atctgtcttt
    95101 tggcatattc ttccatccct acttttttca aagcttgtct tgcacgttct ttttcttttt
    95161 tcccaggtct ttgtccaatt cgtaaagatg gatatgttcc taaaagtaca acgcctaata
    95221 catcaattgg aaaggaaaga tccaattcac ttcgctgttc cacgtaggct atttttttac
    95281 ggactgagtt taccgactgt ccagaaaagg tgactttacc agtagtagct ggtatcaact
    95341 ctagcaaaga tttcataaat gttgattttc ctgcaccgtt tggtccgata ataccggtaa
    95401 ttttttgtgg atttatcgtc aagttgatag ttttcaaaac agttttccct tgataagcgg
    95461 ctgaaaggtc ttgaattaca atggccgtac gttccaaatc ttacactcct ttttaggttt
    95521 acctaacttt ttatttaaat atagtttaac taaaatggaa agcattttca agtagaatga
    95581 tgaaaaagtt tttttcgaat tttcctcgca ttttatgaaa attttctgta gaatcacatc
    95641 aagagttcaa taaggctaac ctaataatga aaaacatgtg ttgaaacgtt tgattatttt
    95701 ttcttgttga ctcaacaacg aaaggagttt ttgtatggaa gaagaaaagt taactcgaag
    95761 tcttagtgcc cgtcatatcc aaatgatcgc actaggtgga acgatcggtg tcggtctgtt
    95821 tatgggtgcc tcctcaacca tacgttggac agggccctca gtgatgggag cctatgcgat
    95881 tgcaggattc tttttgtatt tgattatgcg tgctttaggc gaaatgcttt acgttgatcc
    95941 ttcgactggt tcttttgcaa attacgcgag cgagtacatc catcctgtgg ctggctactt
    96001 gaccgcttgg agtaatattt ttcaatatat tgtcgtagga ataagtgaag tgattgctgt
    96061 aggatcttat atgaattatt ggtggcctga tttgccggca attattcccg gaatcattgt
    96121 cgtcttgttt cttatgctgg caaatcttat ttctgtcaag gcgtttggcg aattagaatt
    96181 ttggttttcg atcatcaaag tcataacgat cattttgatg atcattgcag gtcttggggt
    96241 aatcttattt ggatttggta atcatggtca ggcagtaggg atttcaaatc tttggaaaaa
    96301 tggtggattc ttcactggag gcgtcaaagg atttttcttt gcattatcga tcgttgttgc
    96361 ttcctatcaa ggaatcgaac tgatcggaat gactgctggt gaagcagaga atcctaaaaa
    96421 gacgatcatt gaagctgtcc agtccactat cggtcgaatc ttgatttttt atattggtgc
    96481 tatttttatc attgtgagta tctatccgtg gaatcaattg agtgagattg gttcaccttt
    96541 tgtacaaact ttttctaaag tcggcattac atttgctgca ggactgatta attttgtcgt
    96601 gattacagcg gcattatcag gttgcaattc agggattttc agcgcaagtc gtatggtcta
    96661 tacacttgcc ataaatggaa agatgtctaa aaaatttctg aaactatctc gtcacggtgc
    96721 gcctttttat cctgtcgtag caatctcagg tggaatctta attggtctga tcttaaatta
    96781 tcttctacca atgttcatca accagtcaga aaatattttc gttttcgtat acagctcaag
    96841 tattttgcct ggtatggttc cttgggtagt gattttgatc agcgagattc gcttcaaaaa
    96901 gaagcacgca gataaaatgc atgatcatcc ttttaaaatg ccactatctc cttatacgaa
    96961 ttatgtgaca cttgcttttt tagctatcgt attgattttc atgtttatca atcctgaaac
    97021 gagaatctca ttgattattg gactcgtttt cttgctttac atgacgatcc attactttgt
    97081 acgcgaaaaa agaaccggaa aataaaaaaa gaacgaagct tcaccttagt ggaagcttcg
    97141 ttctttttta ttgcttttat caattcaatt ttagcgtctc accgggagtc aggaaataat
    97201 tctccatgct catcccattc agcgacaaga tcgtttctac tggtacgcca gttcgttcgg
    97261 ctatttgatt tggaccttct ccttccaaaa ctacggttgt cgcagcctgt gaagtcgcgc
    97321 tgttggtatt agtttcagag gaatctgttg tgtcttgtgt tgttcgagct tgcatttcag
    97381 aagcttctac cgactctgct gtctcagaat cagaagatgt tgtgtcttgt gtgcttgttg
    97441 actctgcttg aagagaggcg ctagtctgca aataaatatc ggaaatctta gcgacatgat
    97501 tcgtataatt agccgataat tgattgatca aagtatctaa gtctacaccg tctttatttt
    97561 ctgctgcctg atacgctgca cgtaattttc tgatcccttc gtcggagatt gcttgaagct
    97621 cacgtgcttt ttggttagca actgctgata aaccagctat tccctcttga tttccctgaa
    97681 tttctgtctg gtattcttca atcaatgcag gcgtcttttc ttcgatcttt ttcgaataat
    97741 catttacgat tgcttgaagt tgctctggca aaccatttgc tgttttttct tcagaagttt
    97801 gtgtctcttt tgttttagtt gatgactcac ttgtttgttt cgtctctgtt ttctttgtcg
    97861 ttgtttccgt tatggtcgta cttttcgctt ctgacgaatt ttttccatga aaatatagac
    97921 ccgcaactcc tcctgctatg atcaaaaaca cggcaactgc tgccccaatc atgatcatcc
    97981 gcttacgagc tgcctgtttt tgctgtcttc ttcgggtcat ccgaacattt tctccccact
    98041 cattttcatc atacattttt ttcaccacca atttttatgt ataatagcgt tatcaaacgc
    98101 ttcatattct tattgtagca aataattgtc gctgtgcaca tttttatctt tgttatttat
    98161 aagaaaattt caccagttga gatgattcca agattgtccg tcgatttctc cttgcagctg
    98221 cattgttcgt tcgtctatct cagccatcag tttctttatt tcatataaga gcgcacggtc
    98281 ttgatattct gatttttcgc ttaattcatc taattgttgg atcagccaat catttgtcat
    98341 aatgaagttc ctttcatttc ttgcagtgta aaggtcaagt cttcgatctg ctcttttgcc
    98401 tcagtcagtg cttctacgat atatgcttgt ttttcagatt cttcttcctg cattttctga
    98461 ttttcctgta ctctttgaat aaaccagtca atccgctgag tcagcgatgg tttgccttcc
    98521 aatgagagat actgctgata agcaattagc ttctcccaac gtttttcctg ggataaatac
    98581 ataaattgtg ctaaaacgaa tggcggaaga taggtcatat ggaatttatt tgcgattcgc
    98641 tgatacggcc tcaaaaattc ggaaatcgta tattcttctt ttcctccagc ttggtattct
    98701 ttttccgcca cacctgttgt caatacgatt cctagttctt tcccttttaa tacatgtgct
    98761 gctcgttcca agacattatc ttgccaaatt tttaaatgag caggagaact ataccagtaa
    98821 aacggaaact ggaaaatgat ccgatcatgc tgggccaaga gctgtagttc agtctctata
    98881 tctatttttc cgtctggata acttgattcc aaatgatgcc aagtagctgg taaaccagca
    98941 gaagcttctt tgaaaaattg ctgatttaaa gattcttcga tcgttggatg gctaatgatg
    99001 ataagtgtac gcatcccttt tcctcctttt tagtcttcgc tttacagtgt agcataaccg
    99061 ccacaagttt cttttgccga tgcttgtcat tttaaccaga ctaatcaggt tgtccaagaa
    99121 actgtggttt gtttttccaa ctgttttttc aaggtatcca aagcaatatt cttaaaattt
    99181 ttgtttttca tacgtttacg atagttcgta gccgtttctc cggtactctt tttgaacatt
    99241 cggctaaata cgactagact attgaaccct acttgatagg caatactttc cacggattca
    99301 tccgtcagag aaagaagaaa cgcccctcga ttgatccgta ccccgatcaa atattccttg
    99361 atactggtac ccatatgatt tttgaatact cgcgacaaat ggctccgatt gaccgctaaa
    99421 tgctgggcga tttcctcaac agacaatttt tcagcataat gtgcctcgat gtatttgact
    99481 gtttcaacag ctaaagagga atgaaccttt tgtacaccac cgctgacctt tccgccatct
    99541 tttaaaagga ctgataaaaa acggtaggtt aatgctgtta actgtaattc attttccaag
    99601 gtttcttccg tatctttcat gcattctaaa ataatgtcga tatatttttg tatgtcagaa
    99661 gagaccatcg tgtattggtc ttctctgaaa agcgactgcc gaatgatttc ttctgcagca
    99721 ctcccaccaa atgaaagcca gcaatacatc cacggatctt ctccatctga taagtaaaac
    99781 gtcgagtctc ctggacgaat caagaacaga tcaccttttt tcaattggaa ttgttgattt
    99841 ttcacatgat aagatccttt gccttccatc acgacatgca ggatgtaatc attgcgaata
    99901 gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac cacaaaaatc aaaatacaat
    99961 tctttttcta cttgttgatg ttcctttttt aaaaaaatcg attgctccac gtctatccct
   100021 ccttactcct ttagtataat gcaacaaaaa gttaaataaa agtcacagaa atatattaaa
   100081 aaggaagcga tttcttgtta agtttaagtc attaaaagat ctttgttatt cgttgtaacc
   100141 ggtttactta gtattatata ttgaacagga gaggaataca gattatgaaa agcaaaatga
   100201 tccaacgtgt gtcctacgct ttaggcgctt ttggacacga cacattctat gttacattaa
   100261 gcacttattt tatggtattc gtgacaagcc agatgttcgc aggaacagat aaggcaacga
   100321 atgcaaaaat gattgctaca gttacaagct tagttgtagg tatccgctta gttgaaatta
   100381 tttttgatcc tttgattggt ggcatcatcg ataatactcg tacaaaatac ggtaaattca
   100441 aaccatggtt agttatcggc ggcgttgtta gttccgtact cttggtagca atctttacca
   100501 atttctttgg cttagctact cataatacga ctttatttat catattattt gcaatcgttt
   100561 tcatcttatt ggatagtttt tattcattta aggatatcgc tttttggtca atgattccag
   100621 ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac gtttgctcgt ttcggctctt
   100681 ctctaggtgc aaatggtact actctagtcg ttgtccctat cgttactttc tttacctatc
   100741 ttgttactca taaacatatt gaaggcgctt caggctggtt ctggttcggt gtgattgctg
   100801 ctgttatttc aggcggtact gcgattatca ctgccttggg cacaaaagaa gtcgatactg
   100861 cgatccgtca gcaaaatgaa aaagctggga tcaaagatgt gttcaaagca gttgctcaaa
   100921 atgatcagtt gatgtggctt gcccttgctt atatcgctta tgcgattgcc aatgcagcaa
   100981 caactgccgt attattctac ttcttcaaat atgtacttgg ccaaccaact gaattttgga
   101041 tcgttggtct agttgctgcc ttacttggtt tagtagctgt tccactattc ccaatgctta
   101101 cacgcgtcat ctctcgtcgc tacgtttacc ttggagggat cgcaatgatg atttcttctt
   101161 actttttctt tacgatcgca ggttcaagtt tgccaatcgt tatcatcggg ctagtattgt
   101221 tctacttccc tcaacaattg attttcttat ccgctttgat gacgatcaca gactctgttg
   101281 aatatggtca atggaaaaat ggtatccgta acgaagctgt tactctatca attcgccctt
   101341 tactagataa aatcgctggt gctttgtcta acgggatcgt agggtttgtc gctatcgctt
   101401 ctgggatgac tggtaatgct actgctgctg atatcactgc tcatggcgta acgactttca
   101461 aagcttatgc attctatgct cctgctgctt tgatgatttt ctccgctatt atttttgctt
   101521 ggaagattac attgacagag aaaaaacatg cacaaatcgt tgagaaattg gaaaacaaat
   101581 tggctccagc tgatacaatg gaagatgaac ttcacgatgc agtagatcct tcccattaag
   101641 aaaggatctt gaaaatgaag attacagaaa aagattttgg ccaaggttat cacttgataa
   101701 ccttggagaa caaaaacaag ctagcacttt ctatctctga tttaggtgct agaatcgtca
   101761 gtttaaaatc aaatgatcga gaattagttt tgggatttga caccgctgaa gaatatatcg
   101821 aaaaagatcc gtatatcggt gcttctatcg gtcgcaccgc agggagaatc gaaaatggcc
   101881 acttttctct caatggaaaa acttaccaat tagccacaga tccaaaaact ggacataatc
   101941 tgcatggtgg tgctcctgga tttgaattaa aaaaatggtc ttatgtcatt ttgaatggcg
   102001 aaaatgaagc ttctgtcatt tttactacta caagtcccga cggagaacat ggttttccag
   102061 gtacaatgga tgtggaaatc cgctatactt tgacaaaaga taatatctgg cgagtgacct
   102121 ctcgcggcac cagtgaccaa gatactttat tcaatccgac aaatcatgtt tacttcaact
   102181 tgacgggaga tgcttctcag tcaatcgatc aacatgagct ttggctcaat agcgaagctt
   102241 atgctccttt gcgaacagat agtattccta taggggtaaa agaaaatgct gcaggaagtg
   102301 ctttcgattt tcaaatacct aaaaaattag cgtccgtttt tgctagtgat ttggatcaaa
   102361 aaaatcttgt tgacggaatc gatcatccat ttttcttaaa agaaaccggc ttgggtaaag
   102421 aagccgctcg cttgactagc cccgataaaa gaatccaagt agatattgct acagatgctt
   102481 ctagtgtggt tattttcact gcaaatttcg gcacagaaac accagaaatg cgcaaccgaa
   102541 aactggcaca tcacggtggc atcacatttg agactcaaac agctccaggt gcagagcgct
   102601 tctcagcatt tggttcgatt catttaaaag ccggatcagt atttgaaaca gtgacagaat
   102661 tcaaaatcaa aacaagaaag gaataactca tcatggaaat caaaagaagc ttaaacacaa
   102721 aatttgccga aacattcgga cctaaaaaaa ctacacaata cttctcacca ggacgcatca
   102781 atttgattgg tgaacacaca gattacaatg gaggccatgt cttccccgct tccatcactt
   102841 atggaacgca aggtgtagca gcacctcgag acgataataa aatattagtt tattccacaa
   102901 actttgacga tatcggtgtc attagtttta ctttagatga gctagaatat gataaaaaag
   102961 caaactgggc aaattacgta aaaggaatgg tccttaaatt aaaagaagct ggacactcaa
   103021 tcgatcatgg gtttgagtta ttagtggaag gaacaatccc taatggtgca ggactttcca
   103081 gtagcgcttc attagaatta ttagtaggtg ttgtattaga agatctgttt gatttaaaaa
   103141 cagatcgctt ggaactagtc caaacaggta aaaaagtaga aaatgaattc atcggtgtca
   103201 attcaggtat catggatcag tttgccatcg gcttcggtga gctagatcat gcaatcttat
   103261 tagatacaaa tacgctgaaa tacgaaatgg ttccagtgaa actagatggt tatgcaatcg
   103321 tcatcatgaa tacgaataag cggcgggaat tggcagattc aaaatacaat gaacgccgta
   103381 gcgaatgcga agaagccttg gcacgtttgc aaaaaacttt gcctatctct tctttaggag
   103441 acttagatga agaagaattt gaatcaacta ttgaccaaat cggcgatgat acattgatac
   103501 gcagagctcg ccatgccgtt tctgaaaatc agcggacttt gaaggcaaaa gctgaattag
   103561 aagctgggaa cttagaagca tttggtcaac tgttaaatga ttctcaccac tctcttcgct
   103621 atgattatga agtaactggg atcgaattag atacactagt agatgctgct caaaaacaag
   103681 aaggtgtctt aggtgcacga atgacaggag ctggatttgg tggctgtgcg attgctcttg
   103741 tcaaagaaga aaatatacct gccttcaaaa acaatgtcta tgatgaatac atgaaagttg
   103801 tcggctatgc tccggaattt tatgttgcac acattggaaa tgggacaaca aagatcgatc
   103861 cagaagctgt tcatgtttga taaaaaaatc aacgatctca gctaaaaaca aatagataaa
   103921 aataacctct cagcaatttc caaatgtgag ccttttgctc gtatttgaaa attgctgttt
   103981 tttattgaca cggaaagaga acgtgtgttc taattgtgtt gaagaggtga ttgttatgtg
   104041 ggcgaatcaa aatccggtat ttgtttatga aaaagaacct tcaagagata ttttatgtat
   104101 tgactgcaaa tctttttacg cctccgtaga gtgcgttgaa aaggatcttg atccccttac
   104161 aactaaatta gtagtcatgt cctatccgtc cgattcgaca gaaacacggg gtagcggatt
   104221 aatcttggct tccagtcctg cagcaaaaaa agcttacggc atcaccaata tcagccgtgc
   104281 cagagatctt cccttcccct atccaagtga tttatatatt gttgccccaa ggatggccta
   104341 ttatatggaa aagaacagag aaatcaattc tatctataaa aaatatgcag acgaagcgaa
   104401 ccatcatgtc tattccgtcg atgaaagctt ccttgatgta acaaatagca tggcactctt
   104461 tggtaaaaca aatgcgtacg acatggctaa actgatccat aaagatgtgt ttgagcagac
   104521 cggaatctac acgactatag gaattggcga taacccgcta cttgctaaac tagccttaga
   104581 caatggctca aaagacggaa agcaaatgtt tgctgaatgg cgttatgaag atgtaccaga
   104641 aaagttatgg aaaatctcag atatcactga cttttgggga atcggcaaac gaacagcaaa
   104701 acgtctgaaa caaatgggta tacattctgt ttatgattta gctcacgcgg attattatca
   104761 cttgaaaagc aagctagggg tcatcggtac tcagctttat gcccatgctt ggggcatcga
   104821 tcgaagtttt ttgggacaag aatatacacc aaaatcaaaa agtatcggaa atagtcaaat
   104881 cctagataga gattatacaa gacgagaaga aatcgagatc gttatcaaag aaatggccga
   104941 ccaagtcggc actcgcttga gacgatctgc agcaaaaaca caagtagtca gcttatgggt
   105001 aggatactct ttaggctatc ttgatccaaa tggcaagacc gggtttcacc agcaaatgaa
   105061 aatcgaagcg acaaatgcta gcagagatct agcagatgct ttattgacca ttttccacca
   105121 gcattaccac taccaggata ttcggcgtgt aggcgtgaac tgttccgacc tcgtatacgc
   105181 gacaggttta cagcttaacc tttttgaaga tcctgaaaag cagataaatg aagcgaaagt
   105241 tgattttgtt gtcgatacga ttagaaaaaa atacggcttt caatcaatca tccatgcgca
   105301 tagtctcttg caaggaggac gcgccatcgc acgtagttct ctcgttggcg ggcatgcagg
   105361 tggtatgagt ggattagaag gtggaaaaga tgagacggac agcaaaaaaa ttcattgact
   105421 ataacgagta tcgagatcgg ccatttggat taaaatgggg gactgctttt gcgatggaag
   105481 aactagtcaa cggtatcaaa aaaaatgaag aagaagcacg caaacagcct cttcctaaac
   105541 aacaaatgac attgcaagaa atcgatagta tactgacaga agccttttta tctcgtaaag
   105601 cagtaatcat tcagctcaat ttaaaagatg aatttggaag actcttggat gaggtcgaag
   105661 ggcacttcat tggtgaagcc tatgaagatt actttatgat tgaccatcag ccgatattgt
   105721 gggaagacgt acggtatatt gagttaaagg aaacaaaaaa atggtttgtg acatgagagg
   105781 ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta
   105841 aatttttgtg gaattttggc ttatttttcg aagaagcaac ttctggaaca acgtatatta
   105901 gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg cgctgatttc
   105961 acaacctctg tctatctatc catctattcg gtaagattca tgcatgacgt cttctttatt
   106021 tagtatgtag agcccttttt cttctgacca ttcaacataa atcagctgtt ttacatctgc
   106081 atagccttct ttcttcaagt tctccataag ccatgcttca gtcaattgat tggactgaag
   106141 cactttatgc tgaatttgcc cttcatctac aagcaggatc gataaagctt tgtcttcatc
   106201 tgattttttc aatacactca tttgaccggc attctctagg ataacatgtt tggcattttc
   106261 aagaaaaaag cattcttgct gtcttaagat tgctcgcaac tgctccatct cgatatggtt
   106321 atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt agaacagatg gctcgccttt
   106381 gacccaacga ttgacctttt catttttttg gacgatacgt tcgataccat aaatcattac
   106441 tgcccaaagc gcaatggcaa ataggacatg ccctacatgg acattgtcat cataggttga
   106501 ttcttctaag atcccaccta aaacaagcgt atagactaaa tcaaacggtg tgatttctga
   106561 catggatttc ttcccaagca aacgtaccac taataataaa ccgatcaacc cgaacaccag
   106621 tttcattgta attgaaaaaa tcattttttc tccccctaac tatctgttaa ttaaattata
   106681 tatgctttct cacgaacacg catggaatat acaaggaaga aatgtatcct tttactaaaa
   106741 aaaggactgt gacactgtag tgacccccaa aagttagact tttttggagt ggagaaatcc
   106801 actccttttt tttatgctct aaaatgatat ttttacacaa ccgttacggt tgttttacga
   106861 aattggacg
//
LOCUS       DAFELO010000003        99414 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFELO010000003
VERSION     DAFELO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.003"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            10..588
                     /locus_tag="LP452_000201"
     CDS             10..588
                     /locus_tag="LP452_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012965.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LP452_000202"
     tRNA            complement(828..911)
                     /locus_tag="LP452_000202"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LP452_000203"
     CDS             966..1145
                     /locus_tag="LP452_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012966.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LP452_000204"
     CDS             1294..2511
                     /locus_tag="LP452_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2012967.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LP452_000205"
     CDS             2695..3531
                     /locus_tag="LP452_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2012968.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LP452_000206"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LP452_000206"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL2012969.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="LP452_000207"
     CDS             4976..6034
                     /locus_tag="LP452_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL2012970.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6038..7072
                     /locus_tag="LP452_000208"
     CDS             6038..7072
                     /locus_tag="LP452_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL2012971.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7174..7890)
                     /locus_tag="LP452_000209"
     CDS             complement(7174..7890)
                     /locus_tag="LP452_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2012972.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="LP452_000210"
     CDS             complement(7859..8644)
                     /locus_tag="LP452_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL2012973.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="LP452_000211"
     CDS             complement(8635..9384)
                     /locus_tag="LP452_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2012974.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="LP452_000212"
     CDS             9682..10206
                     /locus_tag="LP452_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL2012975.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="LP452_000213"
     CDS             10222..11721
                     /locus_tag="LP452_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL2012976.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="LP452_000214"
     CDS             complement(11809..12411)
                     /locus_tag="LP452_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2012977.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="LP452_000215"
     CDS             12515..13471
                     /locus_tag="LP452_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL2012978.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="LP452_000216"
     CDS             complement(13776..14732)
                     /locus_tag="LP452_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2012979.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="LP452_000217"
     CDS             14906..15466
                     /locus_tag="LP452_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012980.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="LP452_000218"
     CDS             15459..16283
                     /locus_tag="LP452_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL2012981.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIARYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="LP452_000219"
     CDS             16293..16910
                     /locus_tag="LP452_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2012982.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="LP452_000220"
     CDS             16928..17611
                     /locus_tag="LP452_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL2012983.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="LP452_000221"
     CDS             18294..19577
                     /locus_tag="LP452_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2012984.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="LP452_000222"
     CDS             19593..20480
                     /locus_tag="LP452_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2012985.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="LP452_000223"
     CDS             20497..21333
                     /locus_tag="LP452_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2012986.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="LP452_000224"
     CDS             21336..21959
                     /locus_tag="LP452_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012987.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="LP452_000225"
     CDS             21956..23248
                     /locus_tag="LP452_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2012988.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LP452_000226"
     CDS             23820..24401
                     /locus_tag="LP452_000226"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL2012989.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LP452_000227"
     CDS             24404..25711
                     /locus_tag="LP452_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL2012990.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LP452_000228"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LP452_000228"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL2012991.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LP452_000229"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LP452_000229"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL2012992.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LP452_000230"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LP452_000230"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL2012993.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LP452_000231"
     CDS             complement(29220..29597)
                     /locus_tag="LP452_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2012994.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LP452_000232"
     CDS             29853..30581
                     /locus_tag="LP452_000232"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL2012995.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LP452_000233"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LP452_000233"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL2012996.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LP452_000234"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LP452_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL2012997.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LP452_000235"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LP452_000235"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL2012998.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LP452_000236"
     CDS             33716..35155
                     /locus_tag="LP452_000236"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL2012999.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LP452_000237"
     CDS             35178..36230
                     /locus_tag="LP452_000237"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL2013000.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LP452_000238"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LP452_000238"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL2013001.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LP452_000239"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LP452_000239"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL2013002.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LP452_000240"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LP452_000240"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL2013003.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LP452_000241"
     CDS             39749..40663
                     /locus_tag="LP452_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2013004.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="LP452_000242"
     CDS             complement(40796..42361)
                     /locus_tag="LP452_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2013005.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="LP452_000243"
     CDS             complement(42354..43805)
                     /locus_tag="LP452_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2013006.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="LP452_000244"
     CDS             complement(43819..44835)
                     /locus_tag="LP452_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2013007.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="LP452_000245"
     CDS             45055..46143
                     /locus_tag="LP452_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL2013008.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="LP452_000246"
     CDS             46197..47723
                     /locus_tag="LP452_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL2013009.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="LP452_000247"
     CDS             47725..48930
                     /locus_tag="LP452_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013010.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="LP452_000248"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="LP452_000248"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL2013011.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="LP452_000249"
     CDS             50428..51852
                     /locus_tag="LP452_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL2013012.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="LP452_000250"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="LP452_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL2013013.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="LP452_000251"
     CDS             53425..54402
                     /locus_tag="LP452_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL2013014.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54427..56070
                     /locus_tag="LP452_000252"
     CDS             54427..56070
                     /locus_tag="LP452_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL2013015.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56078..57484
                     /gene="lpdA"
                     /locus_tag="LP452_000253"
     CDS             56078..57484
                     /gene="lpdA"
                     /locus_tag="LP452_000253"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL2013016.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEEFVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57646..57921
                     /locus_tag="LP452_000254"
     CDS             57646..57921
                     /locus_tag="LP452_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL2013017.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57927..58694
                     /locus_tag="LP452_000255"
     CDS             57927..58694
                     /locus_tag="LP452_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL2013018.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58862..60691
                     /gene="typA"
                     /locus_tag="LP452_000256"
     CDS             58862..60691
                     /gene="typA"
                     /locus_tag="LP452_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL2013019.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60952..62724
                     /gene="recQ"
                     /locus_tag="LP452_000257"
     CDS             60952..62724
                     /gene="recQ"
                     /locus_tag="LP452_000257"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL2013020.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62838..63140
                     /locus_tag="LP452_000258"
     CDS             62838..63140
                     /locus_tag="LP452_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL2013021.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63143..64306
                     /locus_tag="LP452_000259"
     CDS             63143..64306
                     /locus_tag="LP452_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2013022.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64332..67760
                     /locus_tag="LP452_000260"
     CDS             64332..67760
                     /locus_tag="LP452_000260"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL2013023.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67781..68905
                     /locus_tag="LP452_000261"
     CDS             67781..68905
                     /locus_tag="LP452_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013024.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68895..69332
                     /locus_tag="LP452_000262"
     CDS             68895..69332
                     /locus_tag="LP452_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2013025.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69316..69654
                     /locus_tag="LP452_000263"
     CDS             69316..69654
                     /locus_tag="LP452_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL2013026.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69773..70333
                     /gene="rsmD"
                     /locus_tag="LP452_000264"
     CDS             69773..70333
                     /gene="rsmD"
                     /locus_tag="LP452_000264"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL2013027.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70330..70821
                     /gene="coaD"
                     /locus_tag="LP452_000265"
     CDS             70330..70821
                     /gene="coaD"
                     /locus_tag="LP452_000265"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL2013028.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70811..71854
                     /locus_tag="LP452_000266"
     CDS             70811..71854
                     /locus_tag="LP452_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL2013029.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72013..72741
                     /locus_tag="LP452_000267"
     CDS             72013..72741
                     /locus_tag="LP452_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL2013030.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72857..73102
                     /locus_tag="LP452_000268"
     CDS             72857..73102
                     /locus_tag="LP452_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2013031.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73099..73317
                     /locus_tag="LP452_000269"
     CDS             73099..73317
                     /locus_tag="LP452_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL2013032.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73453..73992
                     /locus_tag="LP452_000270"
     CDS             73453..73992
                     /locus_tag="LP452_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL2013033.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73985..76258
                     /locus_tag="LP452_000271"
     CDS             73985..76258
                     /locus_tag="LP452_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL2013034.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76296..77342
                     /gene="holA"
                     /locus_tag="LP452_000272"
     CDS             76296..77342
                     /gene="holA"
                     /locus_tag="LP452_000272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL2013035.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77431..77685)
                     /locus_tag="LP452_000273"
     CDS             complement(77431..77685)
                     /locus_tag="LP452_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL2013036.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78085..78354)
                     /locus_tag="LP452_000274"
     CDS             complement(78085..78354)
                     /locus_tag="LP452_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013037.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78824..79279)
                     /locus_tag="LP452_000275"
     CDS             complement(78824..79279)
                     /locus_tag="LP452_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL2013038.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79417..81345)
                     /locus_tag="LP452_000276"
     CDS             complement(79417..81345)
                     /locus_tag="LP452_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL2013039.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81542..82375)
                     /locus_tag="LP452_000277"
     CDS             complement(81542..82375)
                     /locus_tag="LP452_000277"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL2013040.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82567..83271)
                     /locus_tag="LP452_000278"
     CDS             complement(82567..83271)
                     /locus_tag="LP452_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2013041.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83294..83920)
                     /locus_tag="LP452_000279"
     CDS             complement(83294..83920)
                     /locus_tag="LP452_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2013042.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84246..86621
                     /locus_tag="LP452_000280"
     CDS             84246..86621
                     /locus_tag="LP452_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL2013043.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86645..87136)
                     /locus_tag="LP452_000281"
     CDS             complement(86645..87136)
                     /locus_tag="LP452_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL2013044.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87323..88243
                     /locus_tag="LP452_000282"
     CDS             87323..88243
                     /locus_tag="LP452_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2013045.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88397..88741
                     /locus_tag="LP452_000283"
     CDS             88397..88741
                     /locus_tag="LP452_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL2013046.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88931..89431)
                     /locus_tag="LP452_000284"
     CDS             complement(88931..89431)
                     /locus_tag="LP452_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2013047.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89959..90042
                     /locus_tag="LP452_000285"
     tRNA            89959..90042
                     /locus_tag="LP452_000285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89993..89995,aa:Leu,seq:cag)
     gene            complement(90366..91004)
                     /locus_tag="LP452_000286"
     CDS             complement(90366..91004)
                     /locus_tag="LP452_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2013048.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            91339..91773
                     /locus_tag="LP452_000287"
     CDS             91339..91773
                     /locus_tag="LP452_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2013049.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            91796..92116
                     /locus_tag="LP452_000288"
     CDS             91796..92116
                     /locus_tag="LP452_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013050.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            92257..92448
                     /locus_tag="LP452_000289"
     CDS             92257..92448
                     /locus_tag="LP452_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013051.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(92519..92776)
                     /locus_tag="LP452_000290"
     CDS             complement(92519..92776)
                     /locus_tag="LP452_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL2013052.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            92934..93197
                     /locus_tag="LP452_000291"
     CDS             92934..93197
                     /locus_tag="LP452_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013053.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            93221..93409
                     /locus_tag="LP452_000292"
     CDS             93221..93409
                     /locus_tag="LP452_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013054.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(93492..93677)
                     /locus_tag="LP452_000293"
     CDS             complement(93492..93677)
                     /locus_tag="LP452_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013055.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            93892..94263
                     /locus_tag="LP452_000294"
     CDS             93892..94263
                     /locus_tag="LP452_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2013056.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(94413..94485)
                     /locus_tag="LP452_000295"
     tRNA            complement(94413..94485)
                     /locus_tag="LP452_000295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94450..94452),aa:Gln,seq:ctg)
     gene            complement(94531..94959)
                     /locus_tag="LP452_000296"
     CDS             complement(94531..94959)
                     /locus_tag="LP452_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013057.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(95019..95777)
                     /locus_tag="LP452_000297"
     CDS             complement(95019..95777)
                     /locus_tag="LP452_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL2013058.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            96072..96269
                     /locus_tag="LP452_000298"
     CDS             96072..96269
                     /locus_tag="LP452_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013059.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            96375..96857
                     /locus_tag="LP452_000299"
     CDS             96375..96857
                     /locus_tag="LP452_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013060.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(96915..97511)
                     /locus_tag="LP452_000300"
     CDS             complement(96915..97511)
                     /locus_tag="LP452_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2013061.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            97898..99364
                     /locus_tag="LP452_000301"
     CDS             97898..99364
                     /locus_tag="LP452_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL2013062.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        32262 a        17025 c        21142 g        28985 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaatcgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagttact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgccgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgctc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgtagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa
    54481 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    54541 gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat
    54601 gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca
    54661 ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct
    54721 aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc
    54781 ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg
    54841 ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac
    54901 aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca
    54961 gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat
    55021 agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca
    55081 ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct
    55141 gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa
    55201 ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca
    55261 ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa
    55321 agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt
    55381 gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca
    55441 gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa
    55501 cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg
    55561 aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca
    55621 aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac
    55681 ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac
    55741 gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc
    55801 aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc
    55861 atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta
    55921 aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac
    55981 cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct
    56041 gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt
    56101 agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc
    56161 tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt
    56221 gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc
    56281 actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac
    56341 acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt
    56401 aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact
    56461 acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac
    56521 aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac
    56581 tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat
    56641 tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg
    56701 ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt
    56761 caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg
    56821 tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaattcgtta ctgctgatta
    56881 tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg
    56941 tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc
    57001 taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta
    57061 tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc
    57121 aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga
    57181 agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg
    57241 ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa
    57301 cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc
    57361 tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc
    57421 atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat
    57481 ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga
    57541 attatcaata atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac
    57601 ttcctatgct acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag
    57661 tatccacttg atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa
    57721 gatctggaac gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag
    57781 cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa
    57841 aaagcttccg gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa
    57901 actttgagaa tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc
    57961 ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat
    58021 caaatcgaac ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa
    58081 aaatccattc ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat
    58141 tatcggatct cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg
    58201 tgattgaagg ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc
    58261 ttggtttcat ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg
    58321 tgtataaaaa tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac
    58381 ttggtgtgaa tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa
    58441 gcagcatggg gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag
    58501 gaaaccacat tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct
    58561 tattggaaga gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa
    58621 aaagggaata ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga
    58681 aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag
    58741 attaattttc tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata
    58801 attagcagtc gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac
    58861 attgaattat agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa
    58921 aacaacatta gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt
    58981 gcaagaacgt gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc
    59041 caaaaacaca gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca
    59101 cgcggacttc ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt
    59161 cgtagatgcg tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga
    59221 acaacatatc acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga
    59281 gcatgtcgta gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt
    59341 agactttcct gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc
    59401 agcagatcaa gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc
    59461 tcctgtcgat aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga
    59521 ttatgtaggt cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca
    59581 agtagcatta attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg
    59641 tttctttgga ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt
    59701 ctcaggtatg gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc
    59761 gttacctatc cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc
    59821 accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat
    59881 ggcagaactt caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg
    59941 gacagtttct ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga
    60001 gggatacgaa ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa
    60061 atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat
    60121 cgagtccttg agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca
    60181 aatccgtttg acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct
    60241 ttcaatgaca cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct
    60301 tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc
    60361 aacgacttat tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac
    60421 agaagtatat gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa
    60481 catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt
    60541 catcaaaaaa ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga
    60601 atattgcgaa gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc
    60661 tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc
    60721 ttgttataaa aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc
    60781 tttgtcaatg gacataaatt cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa
    60841 acttatttct agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac
    60901 ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac
    60961 ttattaaaac aatactttgg ctatgatgag tttagacctg gtcaaaaaga aattatccag
    61021 aaagtcattg atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc
    61081 tgttaccagc ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc
    61141 tctttgatga aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc
    61201 aatagctcgt tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag
    61261 taccgtttac tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg
    61321 aagcgatgga acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg
    61381 catgatttcc gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt
    61441 cctgtaattg ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga
    61501 cttttaaaaa ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga
    61561 tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac
    61621 aaaaatcaat ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat
    61681 ttgcttaaaa aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag
    61741 cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat
    61801 gcatttggta tgggtagaaa taaaagcaat gtccgttttg ttatccatta tcaaattcct
    61861 ggctctctag aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt
    61921 gaagccatcc tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag
    61981 tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa
    62041 tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc
    62101 agtccgtgca accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca
    62161 gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg
    62221 ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat
    62281 ttatctacct atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa
    62341 tatttgatat cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta
    62401 acagaaagag ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag
    62461 aaagtccagc agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg
    62521 cgaacggatc tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca
    62581 ttgaaagaaa tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga
    62641 gttggccaaa gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg
    62701 gactctaacg ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg
    62761 gttaaatctg ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga
    62821 atgggaggta agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg
    62881 atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac
    62941 aatgcaaagc cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg
    63001 gttttgctgt ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc
    63061 tagaacgaga attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag
    63121 aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta
    63181 tagcatcttg attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc
    63241 tacgtcgtat ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag
    63301 tattttttgg gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt
    63361 tttgaaaaac cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat
    63421 tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt
    63481 ctcgatccaa cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt
    63541 atctaaaaga caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc
    63601 aatggttatc ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt
    63661 tatctttttg attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt
    63721 gattgtagga gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac
    63781 aaacggaaag atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca
    63841 aaatcctttc tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat
    63901 gttcaacgga ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt
    63961 acaagaagca caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat
    64021 ggggattttg atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat
    64081 ccgttcaaaa gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat
    64141 ccaagtattt gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt
    64201 cccattttta agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt
    64261 cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta
    64321 ggagtggttc gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat
    64381 ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat
    64441 attccgtaca ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga
    64501 ttgatgcgta tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg
    64561 ctattcatcc gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag
    64621 aagaaggcat catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa
    64681 tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac
    64741 caatcgcttc tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga
    64801 tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg
    64861 caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag
    64921 tgtatgttga aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata
    64981 cgcatggaaa cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa
    65041 aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc
    65101 aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc
    65161 ttgttgaagg agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata
    65221 cgatcacaga aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag
    65281 gtttggatct gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt
    65341 cagccattca atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag
    65401 ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg
    65461 cttacgcggg ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc
    65521 atggtgctac ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa
    65581 tccgtggagt gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc
    65641 aatcaggaaa tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc
    65701 gactacgaga ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt
    65761 ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc
    65821 taatcacaag aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg
    65881 ctttggtcga atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag
    65941 acgcccatca aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac
    66001 gtttgactgg agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat
    66061 ttgatgtggc ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt
    66121 ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt
    66181 ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg
    66241 atgtatttcg aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag
    66301 ctgtacggga tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg
    66361 atccaagtcg ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac
    66421 aactgggtgc gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag
    66481 cttatcgttt gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc
    66541 atgataccag cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca
    66601 tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct
    66661 tgtattatgc actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga
    66721 aattaaacca ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga
    66781 atgctcctga aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac
    66841 agcaacaggc aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc
    66901 atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg
    66961 gagatatggc actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag
    67021 gcgaaacact aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac
    67081 ccgttggcgg atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga
    67141 atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg
    67201 cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc
    67261 aagtcttttt ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca
    67321 caccaacatt tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg
    67381 gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg
    67441 tactgttctt caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca
    67501 tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa
    67561 cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg
    67621 atacgttgat gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg
    67681 gagaagtcgc acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga
    67741 ttgaagtgac tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt
    67801 ttggctgatt ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc
    67861 aaaacttgaa gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa
    67921 ataccaggag cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct
    67981 ggaaaaggat tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag
    68041 atattatgaa aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt
    68101 cacaactgtt aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat
    68161 gacgatgcaa gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca
    68221 tgatacagaa gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc
    68281 ttttgataac gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc
    68341 agttgtgtat ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga
    68401 aggccttcca cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa
    68461 agaacgaaaa agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata
    68521 caatcaaaca cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag
    68581 gcctgaggaa atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc
    68641 tcaaacgaca gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca
    68701 aaaattagaa aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt
    68761 tttatattgg tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc
    68821 tgtaggcatt gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga
    68881 aagtagtgat agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc
    68941 aagtcgctga aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt
    69001 caatggaaga atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag
    69061 catttgaaaa tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag
    69121 ctgttcgaaa agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata
    69181 gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt
    69241 ctaatgaaat caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat
    69301 gcggaggtaa ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg
    69361 ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg
    69421 cattatgtct caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa
    69481 gaaacagaag aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga
    69541 ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa
    69601 ggtttcgaaa ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct
    69661 cttatgagca gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc
    69721 caagtatttt tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat
    69781 aatatcagga aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc
    69841 aactacagat aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga
    69901 gacagtattg gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg
    69961 ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa
    70021 catcgaaatt acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc
    70081 aatcgaacgg ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata
    70141 tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa
    70201 aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg
    70261 attccatcaa ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga
    70321 gggaaatcaa tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac
    70381 ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc
    70441 aatacttcaa agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct
    70501 gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca
    70561 gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa
    70621 tatgaacgag agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc
    70681 ttattagcaa aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac
    70741 tttggcggag atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag
    70801 cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat
    70861 gttgatcatt ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac
    70921 gatcgatctg aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt
    70981 ctctcttacc tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat
    71041 gacccctttt gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga
    71101 atataatcaa atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc
    71161 gttaaaactt gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt
    71221 agagaaaaca tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga
    71281 cgggaaaagc tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg
    71341 acaaacaatc gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct
    71401 gatcgagtta ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga
    71461 gatcgaatca gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg
    71521 attgatgttc actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa
    71581 gcaaattgca ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat
    71641 cgataaaaaa gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga
    71701 tacgatttca gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc
    71761 aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca
    71821 agaagcgctt gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg
    71881 acacgagttc tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac
    71941 aaaagaaaaa attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt
    72001 cttcaatttt aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga
    72061 acaatgatag aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg
    72121 tctatgatag ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt
    72181 tatagagaaa cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga
    72241 gaagatccat cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac
    72301 accgatatca aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg
    72361 gtctatgatg tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc
    72421 aacttttctg cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag
    72481 ctgccagaac atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc
    72541 gagcctccca aaataaatat caatacagcc actccatcag aacttcagca gattcctggg
    72601 atcggatcag tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag
    72661 aaagtggaag acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa
    72721 aacttcgtta caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg
    72781 ctaatggata ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt
    72841 ttggatagga aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt
    72901 ctatctccaa aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa
    72961 cagtcaagct ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt
    73021 atttattctc attgggacga atgatctagc agagcataaa caactttcta aagaaacgta
    73081 ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct
    73141 ttctttatca cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg
    73201 atcaaagaat atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg
    73261 atttttatac ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg
    73321 atgacggact tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt
    73381 atatttaaag aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga
    73441 ggaatataaa acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt
    73501 actgataagt cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta
    73561 ctgttatctt tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa
    73621 aaaaggatca ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat
    73681 gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca
    73741 ctcctacagt gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat
    73801 tttccatgtt tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat
    73861 ctccatgact atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct
    73921 gtacaccaag ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga
    73981 aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac
    74041 gtcgatgatt atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc
    74101 cggaattttt ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt
    74161 gtgtagcata acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa
    74221 aacaacatct gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt
    74281 gacaatggaa ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa
    74341 cacagaagca gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc
    74401 tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc
    74461 ttacttcagg aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac
    74521 catgcaaaat cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt
    74581 ccaatctgaa tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa
    74641 agatgcagga ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt
    74701 cagtctatcg ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg
    74761 tatcggatgg ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt
    74821 ttctattgca ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt
    74881 gctagtcaaa ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg
    74941 gcttctttta ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt
    75001 catgagtttt ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc
    75061 tcacgtcctg tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc
    75121 tattttaggt agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc
    75181 tggtgtgtta tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgttgat
    75241 agaagaggga ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat
    75301 tataggacgt ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat
    75361 ggataggttg aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat
    75421 ttttttgact ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca
    75481 aggagacagt atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg
    75541 aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga
    75601 aagaaatatc gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt
    75661 gacacacgac gatacggatc atgtgggaga attacccacg ttatctcagc attttactat
    75721 caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc
    75781 tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg
    75841 ttattatgat ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc
    75901 gattggtcta tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca
    75961 agcaatggaa aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt
    76021 aggtcatcac ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa
    76081 acatgggatt atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt
    76141 ggagacacta gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac
    76201 ctatcgctgg catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta
    76261 attctcatct gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag
    76321 cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag
    76381 aaaagtttct ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg
    76441 aagatgactt aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag
    76501 ctgaagcaga gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt
    76561 attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg
    76621 cttatttgaa agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt
    76681 tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc
    76741 aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa
    76801 tcaaaatgga acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca
    76861 aaatgatgag agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg
    76921 taagagaagt agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc
    76981 aatatatttt aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc
    77041 aaggggaaga aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac
    77101 agacaaaatt tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa
    77161 tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc
    77221 tggtacagtt gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg
    77281 aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat
    77341 aagaagaaag acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac
    77401 aaataaaaaa atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga
    77461 tttgtcgcgg ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc
    77521 tttaacagct tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc
    77581 aaattttttg attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt
    77641 ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga
    77701 taacttaagt tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc
    77761 agcgtgcttt gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt
    77821 ttttttcagt aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga
    77881 ggcaatagcg tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg
    77941 aatgtttggt actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa
    78001 agggatacga ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa
    78061 ccagtatcct ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc
    78121 gatatttttt atcaacattt tttttatgac caactggttc cgacggttcc atttgccact
    78181 tacttccagc acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc
    78241 tttaagcagg ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat
    78301 tggctgttct tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt
    78361 cttcctttct tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt
    78421 tgaagtggat gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc
    78481 agaagagcca aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct
    78541 aaattattac aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc
    78601 gttttaatag gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa
    78661 tagataagct aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat
    78721 gaaatacttt aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta
    78781 aattgggggt gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa
    78841 atacttgtaa gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc
    78901 ttaagaagac aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag
    78961 aagcaatcaa tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag
    79021 ctacccagcc aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg
    79081 ttgcgatagg gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc
    79141 cttgaagcca agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag
    79201 ctgccgctat catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta
    79261 aaaaaagcca actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt
    79321 ttttagaaag ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt
    79381 tcataaaata aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc
    79441 tttgtatagt ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct
    79501 tagtaaacgc aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc
    79561 aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat
    79621 cgaaaaaata atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta
    79681 gacaaattta tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc
    79741 tgtcccttgc cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac
    79801 tccatcacca accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt
    79861 tttttcttcc ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc
    79921 ctttgctgtg ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa
    79981 gtaggaaatt acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat
    80041 taactgctga tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat
    80101 catttccgca atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac
    80161 tttccattgc gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg
    80221 taagtattgg atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag
    80281 tggatgcgtc gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc
    80341 aaagaaaaag ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa
    80401 ggcaatcgct tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc
    80461 attttttgca gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc
    80521 acaaggagaa gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca
    80581 acctaggaaa aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac
    80641 ataattattt tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc
    80701 ttctactaat tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat
    80761 cgtgatggga tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc
    80821 tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa
    80881 aaagccatct atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag
    80941 ttgagaaaca tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg
    81001 ttgaagattc atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa
    81061 tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa
    81121 acaggcacca atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca
    81181 caattctttc aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata
    81241 aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa
    81301 aaggcagccg attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc
    81361 ttgtcaaaac aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga
    81421 acgataatgg ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatgatt
    81481 attattaaag aaaataaaaa accagaacat ttctcaaata agagaagtgt tctggttttt
    81541 tctacattgc aataaaccaa taaccaatca aaatgatacc aaggataata cgataccagc
    81601 caaaagcggt aaagtcgttt ttcttcaaat agcttagtaa gaatttgata gctaagacgg
    81661 aaaccacaaa agatacgaat gttcctacaa gaagaacaac agtttcttcc attttgaatg
    81721 aattcccttc agctaaatat ttgacgatct tcaagccgct tgcgccaaac atgacaggaa
    81781 tccctaagaa aaatgaaaac tctgtagcga caaaacgcga agcaccaatc aagatagatc
    81841 ctaggatcgt tgcgccagaa cgggatgttc cgggaacaag tgccaagact tggaataacc
    81901 caacaagaag agctgcttgg tacgtaaagt cattcagaga agtccaacga ggtgttttgt
    81961 ttttgttgcg tttttcgaca ataataaagg caataccgta aacgatcaaa acaatggcaa
    82021 ctggtaagaa cttgtggaaa tttgcttcta accaatcatc caaaggcaaa ccaatgatga
    82081 tggctggtat gcaagcaacg atcactttaa accaaagtgt ccacgtatct tttttttcta
    82141 ccgttgattt acttggagaa aatgggttta acttgtgaaa ataaagaacg acgacagcca
    82201 agatcgcccc taattggata acgacattga acatcgacat aaattgtgaa ctggcatcca
    82261 gtttgatgaa ttcatctgct aggatcaaat gaccagtact actgatgggc agccattccg
    82321 ttattccttc aataataccg agaatcactg ctttgataat atttgcaaaa aacataaatt
    82381 ccttccttcc caattaagat acatcttata gtataccgtt attttttgag aaacccaatc
    82441 tgttttttaa tattaagaaa aacttgagaa ataaagcaga taagtacacg aagagaaaaa
    82501 agaagacaaa gccctcgttc agactttgcc ttttatgatt ctctggtaaa aattaaaata
    82561 tccccattat agaaacgagt cgcgactatg tgttttcccg ttatcgatat aagacgtatc
    82621 attgatgccg tggatagaaa atcgaccgtt tgcataagtg acctttgtta cgctgccatt
    82681 taaaagccca gcacgaacag gctgtttagg atcgatcaga ttaagcaaaa aggcgatcgt
    82741 caaaccatga gatacgacta aaacatttcc accaccattt ttttcgcagt gatgggcgat
    82801 atcttcaaat cctgtccata cacggtctcg caatgtttca taagtttccg cccacccagc
    82861 ggtatctaag ttgtatacag cattagcaat ttgttcaaaa gaaacttctg tggtaaacat
    82921 ttcatcatat gttttaaaat tcaatacacg agggatcaca ccccacattt ccatgtcata
    82981 tccaccttca agagacccga aacaccattc tctaatcctt ttttcaataa aataaggaat
    83041 ttcttttccc ttagaatgtt ctcctagaat aatacgggca gtttcgattg cacgaccact
    83101 gtcactagaa taagcaaatg aaaagtcgac attttttaac ccgcgaccta aataatgaat
    83161 gccttcttgt ccttgctttg tgaggggtgt atcacaccag ccttgtactc gttcaattgt
    83221 attgaacatc gtttttccgt gtcgtacgat ataaagtgta gtttctgtca ttttattctc
    83281 ctcctaagtc gttttaacga ttgaaaaaag gatttgtcct tttttcctgt tcaatcgtcg
    83341 ttgcatcgcc atgtcctgga tagactgcaa attcatcagg taatacaaac agctgggtcc
    83401 gaatactatg aagtaattgt tctaaattcc ctgtatacag atcggttcta ccgatacttc
    83461 ctctaaacag ggcgtcgcca gaaacgacaa aatcatcaaa aataaagctg acgcttccta
    83521 tagaatgccc tggggtaggg acgacgcgaa aagacatgtt tcctaaagta taagttttca
    83581 tttcaaattc atattctgca ggcgagacaa taacatcagc aatatcatca tgtctaccga
    83641 gtccagaaag attcaaaatc ggatcaccta gccatttttg ctctagtggg ctgacataaa
    83701 ctgggatttg ataatacgcg cgtaaaggtt ccactgcacc gatatgatcg tagtgagtat
    83761 gggtaagcaa aatcgctacc ggctgttggt ttgttttttt gatttcttgt ttgattcgtt
    83821 ccgcatcttc tccagggtca atgatcaaaa gtgcttcttc gttgtagacc aaataacagt
    83881 tttcttcaat cattcctgtt tttaatcttt cgatatgcat gtgcattcct ccttgatagc
    83941 caaaacttct ttcatccatt agtataacgc attcattgct aggaaacaaa aacaggtact
    84001 tatttctctt tacttatctg aattatcaga ctatatgaaa agtatttgga tattctaaag
    84061 agaaggaaag taagttttca tagcatttta atatgtcctt gttacattag attcatccaa
    84121 aaatccaaat tctatatcct atatccctgc ctaatcagca gggtgttttt ttatgtttgg
    84181 aaagagagct ctttttgcta aactaggttt tttcaggtaa tcttagagag aaagaggtgg
    84241 tggaaatgat caaacagtca aaaatatctg tgcggcactt agtagaattt attttgcgac
    84301 gtggaagtat cgacaatcgt aaaaaaagta accatactgc attagaagga gcgagaatcc
    84361 accgcaagct ccaaaaagat gcgggagaaa actatgaaaa agaagtcttt ttaaaaacaa
    84421 cagttcaatt agatgaatat tacctgatcg ttgaaggccg tgcagacggt attttcaaaa
    84481 gagacggtat ttattatata gatgaaatca aaacctccga accacgattt gaagatttag
    84541 aaccagaaca agtggaactt ttctttcatc aagcaagggt gtatgcctat atctatagtc
    84601 aagaacatga tttacaagaa ataaatctgc aattgactta cttccagacg gcagaagaag
    84661 tgatcacgag aaaggttgaa catcagacaa gagagcagtt agaaactttt tttaaaaagc
    84721 tgactgaaga ttataaagaa tggcttgttt ttcaagaaaa ctggcgcact gtacgcaatg
    84781 cctctttgat ggctttgaaa ttcccccatg atgagtatag gaaaggtcag cgtgaactag
    84841 cggtagctgc ttataaaacg atccgtacga aacagaaatt gtttgtagaa gcacctaccg
    84901 gaacaggaaa aacaatctct acgttattcc ccgctttgaa agctgtaggg gaagaagagg
    84961 gagaaaagat cttttatcta acggctaaaa caataactag acaagtagct gaagatgcta
    85021 tgacagcatt aaaagataca ggagctgaag taaaaagtgt cactttgacg gcgaaagaca
    85081 agatctgctt tttagatgag acgacctgta atccagatca gtgcccatat gccaatggtt
    85141 attacaatcg gattaatgaa ggtttatggg atcttttgaa tcatgagaat cagatcactc
    85201 gtgaagtcat cgaaacttat gcaagaaagc atacactttg tcctttcgaa ctatcattag
    85261 atgtcagtat ctggtgtgac gtaatcattg gggattacaa ttatttattt gatccaactg
    85321 tttatttaag acgttttttt gaagatgaaa aaaacgagga ctatctgttt ttgattgacg
    85381 aagcacataa tttggtgaat cgttcccgag agatgtattc ggcagaacta tcttatgaaa
    85441 aaacgaaacg aagtaaggaa gctataccaa aggaattcaa aaaacttcat cgccgtttca
    85501 acaaactatt aaaagaattt gattcgatcc gagaaatagc aaaagaagat cattgggatt
    85561 atcatcatca aaaagcaccg gctgaatctt tagtaaaggc aggttatcaa ttaagcgaaa
    85621 aaattaaaga atggctggca gaatttcctg agcacccatc acaagaacaa ttgcttcctt
    85681 attattttga tttgcttcat tttttgaagg tcagtgaatt ttatgatgat cactacgaaa
    85741 caacagtcga aaaaacgtat cgcgatctga tagtaaaaga gttttgtatt gatccaagct
    85801 tgtttcttga gcagtcgttg gataaaggaa aaagcagtat tcttttttct gctagttttt
    85861 caccactctc ttattatcag gaaacactgg ggggacaaaa aagtctggct tacaaactgc
    85921 cgagtccttt cccagaagga aaccagcaag ttttcatcgc aaattaccta gagacgactt
    85981 atcgaaaaag ggaacacagt ctggcaaaat tagtagagac gattcatgct tttgctgatg
    86041 gaaaggtcgg gaattatttc gttttctttc cttcttatca atatttagat cttgcggtgg
    86101 aagcattcag acagcaatat cccgacagca atgtgctaat ccaagaaacg gacatgaatg
    86161 aggaagaacg cgaacgattt ttaacaaaat ttcaaacaaa tcccgctgaa acattgattg
    86221 gattttgcgt acttggcggg attttttcag aaggaatcga tttgagaggg gaccgattga
    86281 tcggcagtat gatcgtaggt gtaggtttgc cgcagatgaa tcatgagcag gaactgatca
    86341 aatcatatta tgatgagaag gaacaccttg gatttgctta cgcttatcag cttcctggaa
    86401 tgaacaaagt cttgcaagct ggtggtcgag tgattcgtgg catggcggat caaggaatcg
    86461 ttgtattggc agatcaacga ttttctagtt tttcttatcg acgtttattc ccacaacatt
    86521 ggcaaacagc aagagaagta cgaagtgttg gggaattgga agaaggaata cagcgttttt
    86581 ggttatcaaa agaagcagta aatcaaaaaa acgaggaatg acagacgtca gcctcgtttt
    86641 ttgattaacg atagttagtg aattgcaact cgattggtaa gtcaagttta cgtaataaag
    86701 caatcacagc ttgtagatca tcacggttct tacctgtcac tctaatctga tcttcttgga
    86761 tttgtgcttt tactttcagt ttttcatttt tgatagctgt agtgattttc ttcgcgtttt
    86821 cacgatcgat tccgttgacc aaatcggcag tttgtcgagc tttgccacct agagcgtgtt
    86881 ctgattcgga aaaatggata tttttgattg gcacatttct tttgatcagc ttaccaaata
    86941 atacatcttt gatctgttcg attttgaaat catcatcgct caatacaact agattgctgt
    87001 tctctaattt gatttctact gtagaatcct tgaagtcaaa gcgattggac aattcttttg
    87061 ttgtttgctg aatcgcattt tttacttctt ctgtattgac ttctgataca atgtcgaagc
    87121 tagcatcttt tgccatagga accctcctga tatacatata aatatttacc atacatctat
    87181 cttatcatag gattagggca gattcttacc tgtttgctaa aattcttctc atttgagttc
    87241 gaagtatttt attttaatag ataaactgtt attcttacat ttggaatacc aaagtagggg
    87301 agaggtgctt gtgagtaaaa ttatgactgc gtatcataaa aatgaaagat taaagaaaat
    87361 agtggttatt ttgataaccg gattgaccgc tgcggtcgct ttaaattatt ttttgattcc
    87421 agccaatgtc ttttctgcag ggatgaatgg gatcgctcaa atcatttcct cgcttttata
    87481 tgaatggttt catatcaaga tagacacagg aacatttatt tttcttttga atatccctgt
    87541 gtttgtatta gggtttctca aagtaggaaa acgagcaacg atccttagtt ttgtgaatgt
    87601 tgtatgtgta tcggtgatga cgatgctgct tccagtcgga cagatcactg ataatatttt
    87661 gatgaatgcc ctagtaggcg gtgttttatt aggtcttggc gtaggattat ctttaaaaat
    87721 gggctttaca actggtggga tggatatcat ctctcttgta ctttcacaaa caactggaaa
    87781 aacagtcgga aactacatgc tgattctaaa tggtatcatt gtagtcatcg caggcttctt
    87841 ctttaactgg gaaagtgcct tgtatacgat tatttccatt tatgcaatga ctcaagtaat
    87901 tgatgccatc cacacgagtc atcaaaaagt aacagcaatg atcgttaccg tcaagccaga
    87961 agaggtaaca gaagaactag caaaacggat ggtacgtgga atgacattac tgccgtctgt
    88021 cggtggctat tcaaaaaaac aagggaacat gatcatgatg gtcatcaccc gctacgaaat
    88081 gtatgatctt gatcagatcg ttcatgaaat cgacgacgat gcatttatca atattttacc
    88141 aactcattca gtatttggcc ggtttgcaaa tgaaaacgaa caaaaaatgt atcgaacaac
    88201 tggcgtattg ccagaaataa aaaatcgttc acaaaaaaaa tgaggaatca ggtttatttt
    88261 agaataaatt taatctatta catctaataa gaacattttt ataaatttaa acgagaatct
    88321 tacttctaaa ttctcattta caagcagcta aaaaaagagt atgataaata aaaatcgtgt
    88381 taaggagtgt cgtacgatga gcgaagcaaa ccaagaacgg tctggacaag aaatagaact
    88441 aatcaaagaa cgtattcttg ctgccttgga aatggtgatt gatcctgaat taggaatcga
    88501 catcgtcaat ttaggcttga tttatgatat agaattcaat cctgaaaatg gagaaacagt
    88561 cattaagatg actctaacaa ctatgggctg tccattagca gatattttaa cagaatctat
    88621 ccatgacgca ttaaaagaag tgccagaagt aacgaaagca gaagtcaaac tagtctggta
    88681 tccagcatgg acgacagata agatgagccg ttatgcacgg attgcgttgg ggatacgata
    88741 gaaatcttgg ctgactagct atgagaagca aataggaaaa acaaaaaagc cttttatcct
    88801 ttctagaaga aaagagataa aaggtttctt tgtttataaa attttgtctg ctatattata
    88861 agttaattaa ataaatgtgt tctattgata aaggacattc aaatcccttt ccttttggaa
    88921 tccttctttt ttacatcagt ttgattttcg tatagtattc tttctcatct tcacgataaa
    88981 tcgcagcccc atttttttgt tgtgctttaa gactgccaag atttttttta tttacagaca
    89041 aaagtgcctc atcaatgccg attttttttg cctcttgtag tactaatgct aatccttgtg
    89101 tggcatatcc ttttttacga taattagggg agatgccgta accaatatgg ccagcgccgt
    89161 ttcttaatgt ttcgttcagt ttatggcgca aattaaaaat tcctacatac tcatgttcat
    89221 cactttctaa aataaagacg gttgcgggaa cgtaactctc aggaagatcg attcctaaag
    89281 acgttttttc gcattttttt acataatcta aaaattcagc aaatgtatag ccataagcat
    89341 tgttgataaa tccactctca tttggtccaa agctagtttg tagctgataa gctttcttta
    89401 aatctgcttc ccatagtttg atcagtttca tcgtattcac ctctttaagt aaccttattc
    89461 atttgttttc tactatagca ctattgttgt aaatagccat gcctattttt atttattgtg
    89521 tcatatcgta ttaagaatca tcgaacaata caaatatgaa ttacaataaa aaaattattt
    89581 gaagttcgtt cattcgttac ttatgatctc tagaagaaag aaaacgtgaa ttacttcagc
    89641 gtctagaatt ccagaaggat agtaatcaac agaagaaata atcaatatcc aaatgaaaga
    89701 agatagctga atcgagaaaa agaggaaaaa agaaatgaag aaaaaaataa aatagttact
    89761 aatcccagag tcgttcgttt aacattgaaa tcgatcatat ggataaaaca gattttttca
    89821 ccaaccctca atagatgatg gagttgctcg gatgaattat ttgtgtagat ggcataaggg
    89881 agggcgtaca gaaatgaaaa agaacaaaaa tatgttcttg acaaaaaaca catgtaatcc
    89941 taaactaaaa gatgtgatgc ggctatggcg gaattggcag acgcgctagc ttcaggcgct
    90001 agtgagggtg acctcgtgga agttcgagtc ttcttagccg cataatttgt ttttttataa
    90061 agagaaggta cacctggagc gatgagaaag aaaataacat attatctaaa agaatatagg
    90121 aaaaatttta aacaacggaa ccagattcaa ataaatcaaa aaaaatttgt gtgaaggtgg
    90181 gacagtttaa aacctaatag attgattcaa atagtagatt tatagattct gatacaagaa
    90241 atactggcaa acgatagtta aggaataaag actaaactta aactcaaaaa gaggtcatgc
    90301 gaaagaataa atcgcatgac ctcttactat attttgaatg tgtttaaacg ttcaaataat
    90361 gaatcttagt accaaccgtt tgcttcccag aatgctttcg ccgcttccca agaaccataa
    90421 cgtgaagtaa catattgttc tgctactctt tcttggtttg cagcagagta gtcaccattt
    90481 aagtaagaag aatctaattg gtaacggccg atgtaacgtc cgttcgttgc tgtgtaagaa
    90541 ccgcttgatt ctttttgtgc gatccattct tttgcagagc ttgtacttgc tggagtagca
    90601 gcagtttctg ttacagcagg agcaggttgt gtttcagcaa caggcgctgc ttgtgtttcc
    90661 gtttgaaccg tttcttgtac aacaggttgt tcagcttgaa caggttcagc ttggactggt
    90721 gcttcttgaa cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt
    90781 gttaattgtt gtcctgagta gatcaggtta atatcagaaa ttgaatttga ttctgcaatg
    90841 gcattgatca aggagttatc accaacgtat ttttgagaaa tcgttgatag agtatcacct
    90901 gattgaacgg tataagcttc gtccgcatga gcagtcgttc ccatgaagaa tgcagcgccg
    90961 gcagccaaag ttgttccaaa aagtaaagtt ttaagtgatg tcatgaaaaa tctcctttat
    91021 tcgttaaaaa gttttatttg ttgtgttcaa cgaggaatac tttaacacgt gaaaatattt
    91081 cataggtaaa agaagcatga caatttatga caaatccacg ttttttgtaa cagaatgatg
    91141 ttcgctcgat gtgaaaaatg atgaggaatt tcacataatg cagaaataat gggattagaa
    91201 caggtgttct gatgagaaaa gaaatgtata aataacttga aagaaattgc ttttgttcat
    91261 cattttattt acatattgcg tatatttttt gtatttttgc ttgagatgtt acaataaaag
    91321 aaaaaggagg cggttccaat gagtaaaaga taccagaaca tcatggtagc agttgacgga
    91381 tcaagacaat cgatcaaagc gttcgctgaa gcccttgact tagcaaagga caatgaagcc
    91441 cacttattta tcgtgtcaat catcaacaaa gttgagctga cccatagtgc ctatgccttt
    91501 tcaaaaatct atgcagatga aaaacaaaga acagaagtag ccatgctgaa aaaaatcaat
    91561 gatgccaaag agtacggcat tgctgagatc cacgcaatag ttgaaactgg agatccaaga
    91621 aatttgattg ccaatgtcat ccctggacaa gaaaatattg atttgatcgt tatgggagcg
    91681 acaggaaaag gtgctataca acaagcatta gttggatcga ccgcttctta tgtcgtatca
    91741 catgcccctt gcagtgtgtt ggtcgtgaag taatgagata agggaggaat agaaaatggt
    91801 tcgtcctcaa gaagtccgtg cgccaaaaga aaaaatagaa attttagcaa ttattgaaga
    91861 cggcacacaa acaaagaagg gatactcgat cgctttagta aagtggaaag gaaaaaaagg
    91921 tgttgctctg agatgggatg gagataacca acaagataaa ggattccctg tcacagctaa
    91981 cggttatcat ccagcgtggt ttgttctgcc tgataaattt atggaattat acagctatga
    92041 ttatgcaaga gccgcagcat caatcaaagc attagataaa ttaacagaag agcagaatga
    92101 aatggaggaa aaataaaaaa gacgaaccaa tcagccttta gaaaactgat tagttcgagg
    92161 agtaaaaatg aaaaaaggtt atttgggatg aaactagaat aatgcgtata acttaaataa
    92221 aacttaaagc aataaaatgg caagagggtg aggcaaatgg aagcaggaaa agtattacag
    92281 gaagtaaaag cactatgtgc agataaaaaa tatgaagaag cgaaaagcct gatcgaaaag
    92341 aacaaagaaa atttggaaga aaagtacccg atcgcacaag aatttataga attaaaacaa
    92401 ggaacgatcc ttcaccgatt caaacatttt tttggtataa cagaatagaa aaaacgatta
    92461 aagatcggca gaaatttgct gcatctttaa tcgttttctt tttaaacatg cgagggattc
    92521 aattctgatt cttatataaa aaatagatcc aaataataag aaaaattact gaaagcgtca
    92581 attcgatgat gtgtgttatt aatacagacg ttgtttccca atgcctaaaa gaaaaaattt
    92641 gataagtaaa atatacagca ggtaaagagg ccagtaaaac tgaaaacttc ttttttgtcc
    92701 attttatgta atagaaaaga atgcttatca taataaaaaa aggaatataa aaaacaaatt
    92761 gaccgatcgc gatcatacta ttgcctccga taaataaagt ccatgataca aaataacact
    92821 ttgtgcgaaa tggcgcaagc agttttgtgg atttatccgg taaaatctgt atcgattttg
    92881 aaattagagg agaaacttgc tataatcaaa gtgaaagaag gagtgggatt ttcatggatc
    92941 atgtagtgtt agaaatcgat ttgcatttga tcaataatat tcgggtgatt tactatgttg
    93001 taagtaacag tgtcgaacaa cgtgtactga cgaataaaat aaatggtatt ttagcaaaga
    93061 aagacgttca ccgattcaat aatggtgaag gaagttatta tagtatccca gtagaaaaaa
    93121 ttatttatac gacagtcaaa gtcagagaag atctagatac caaaaaagca tatgaaccta
    93181 tttttacaac ttattgattg acagcagatg gaggaaaaaa atgaatccat atgaacaact
    93241 tattcaagat ttgatcgatg gaaaaatcga aaagattgat gtaaaaagag aagaattaat
    93301 ggcttttcga gaagcgtggc tgaagttgga gaatcgtaaa tatgtggtgg gaaaagcctc
    93361 actgaacggc aatgtgactt atcattacga tccgacacgt ttattttagt atgaagtatt
    93421 cagaaaagaa ccaaaggagg acgatgcagc agcattgtcc tcctttgatt ttacaaaagt
    93481 gaaagtttta cttatttgaa tagattttta aatttgtttt tgacactgtc gatatttaca
    93541 tcttttaaaa gattttgggc tttctcttta taaggtccta attgttcttt atgttcttcg
    93601 ataaatcgtt ttgcgccatc gaaattgccg ttcttaatca tttccttgac tttttgaaat
    93661 aattcttctt gattcatttt gaacacatcc tttgctatca acatgatttt ttacatggac
    93721 aaaacgagaa aaactgattt atccacacgt caataatatc aaataacgat catttttact
    93781 aaaaaaacac tcgattctcg cttctaaagt aggagaaaaa tctccaactt gggacggatg
    93841 gaatttcttt aactgaccca tatactgaat atataacaag gaggtagaaa gatgaaagtg
    93901 attaaatatt tgattggact ggcggcgatt agtggtattt tgctatttgg actgaaatat
    93961 tatactgaag gaagttatgg agaaatccct ggtatacttg atcagctaaa tccattagtt
    94021 gaaaaaggag aggtctatgt aaagacacaa aaaccagagg aagtaaatga atatggtaca
    94081 gcaaggtatg tgcagaaagc tgctgatgcc aatgggaaag aacgaacggt tgaatttaat
    94141 ggtctttctg tgttaaaaga aaatcattat ttgaaaatta gcaataaagg agcacacata
    94201 gaaacgtatg aggaagttcc tagagaaaaa gttccggaga aagcattagc aattattggg
    94261 taaatcagtt tttgtattaa gaatacgccc gattttatta ttcataattt aattatgtaa
    94321 actaaataca agtagtaaaa ataatacaat aatccccctg caaaaagatt gttcttttac
    94381 agggggttca ttttttcagc aagcacatca aattgggatg gcagggttcg aacctacaat
    94441 tgcttgattc agagtcaagt gccttaccag tttggctaca tcccatttat tcaacaaaat
    94501 aatcatagca ctttcctaaa aaaacgcaag ttatttttct tgatcggata gttgttgctt
    94561 tactcgtaaa aggcggcgta ctagtttctt ttctaatcca gcaggaaggg caatcagttc
    94621 tgctactgac aagacggtgt ccaataactg atattctttg acaggttcat tcataatatg
    94681 atagatgttt gctaaacgtt caatatattc aaagcgagta tactctgttc gagcaattag
    94741 aaatttcaat aaatcggtgg ctttctcgta ctcttgatct gcgtaataaa caagagattg
    94801 acgatacaat ttgatttcca gttcattgag ggtagcaggt ctgcgacgct cgttccactc
    94861 attaatagag actttacctt gaacatttcc cttgcagtca cggacaaaaa taatcggaat
    94921 ttcatttgga tccatttttt tatgtgtgaa tttttccatt atctacgctc ccttcaaagg
    94981 tttggtatct tgagtataag ggatttttgt ttgaattact agcttttctc caaagttagc
    95041 atctgtccac ccattgcagt gaaaatcgcc gaaaaatcgt tgatcggtac tgctcgattt
    95101 tttacaccat agggatcgtt gacataaata ttttctttgt catatccagt gactacacaa
    95161 gcatgaacaa gaggagtgac tcttacttct ccattatttg tggtccaagt cataaaatca
    95221 gtttcatctg ggacttcaaa attgatagtc acagaagtcc agacaggatt tcctttagat
    95281 agtgcttttt tgatatcatc gagttgagca tcggtttttg gaaaagtgtg ataatccgtt
    95341 tggacgattt gctgagcaag ggaggcaact gggggaacat agacgaccat agcccagtct
    95401 ccgccactga tatcaccaac aaatccttca ttaggatcgc catgagtccc atctgcattg
    95461 aaaacaggga cgtaatccaa ttgctcagct aactggtttt tagttgtttc ataaccataa
    95521 aaagataaaa gcatagaaag cgaagtgact tcacagccat tttctaaaga aggagaatca
    95581 aactggctct ctaatggaac atctagtaaa acttctgctt tctcttttgt ctttttcgtt
    95641 ttttgtgttg aacttttttg tgcagtgttc attgtatgtt ctggagaatg gatatgtagg
    95701 taataacttg taccgcttaa taaaagagca gctgtgcccc ctagaaacaa tagttttttg
    95761 aacgtttttt tcttcatttg aaatctccct tttcagctgt ttatctttcc tattataaat
    95821 gtatttttat tggaaatgga agtattatat tttaatatgt aggaaataat tagtttttaa
    95881 ggaatatgtt ttaatttttt gaaaaaaatt cttctaaata caacagctgt tttaattgtg
    95941 aaaacgaaaa cagtacgcta taatgcctgt aaggttgatt tttaactgta aataaaaatc
    96001 aatgagttaa atctccaaag aaatagcagt ggtaatgtgg agaaaagttc cagcagttta
    96061 ggaggatttt gatgacggaa gaaatgatta atttaggaga acaatatgca tgcaaaccaa
    96121 ttggttttac aaaaacggtc attggtgaag ttgtatcaaa aatgacaaac tgcgccgtgg
    96181 tcaaagttgc ccaatgtgct gcagaggacc aagaattatt agatgaaaaa gcaagcatgg
    96241 ttgttgcaaa atatgataca tttgaataaa gaaaacaatt ggacgtgcgg cgtgctgcaa
    96301 gtccaatttc tttgagaagg taaatcagta ttcttttcct attttctgga gaagaaaaag
    96361 cgaggcgagt tgtcatggta acattcttta cggttctatt gtttttattc gttgttgtga
    96421 tgtttttatt tctttggctg gttcgaaaag aaattattta tcggactgtg cgaaatcgat
    96481 gggtctactt ggtgataccc ttcttagtgt cactagtcat ttggtataca ctcatcagtc
    96541 atcctacagc agatgaattg gcaaaaggga tcttgtcagc aatgattttt attagttttt
    96601 tgttggacag tagaggaatc acagaagaag gacttgtttt gaatagcttt gacaaaaaag
    96661 gtgttccttt ttctgagatc aataaaattg tattatatca gccaaaaggt tctaaaatcg
    96721 taaaaatgaa ttttttccga aacggatggc gaggaccgat gctgaaattt tctgcatcat
    96781 tagaagaatt agttccattt ttgtcccaac gcttaaacga tgaggcagag attgatatta
    96841 tgattgatcc agaataaaaa cagaggttgc gacaagatgt ttgtcacaac ctctgttttt
    96901 tatttcaaat aaaattatcc aaatacgcgg aagcgatcgg catcgttcat gtattctttt
    96961 tccccagccg gtgtttctgg tgaaccgaat actaattgcg aacgtagttt ccagtttgaa
    97021 gggatattcc attcttttgc aactgcttcg tcaataactg ggttgtaatg ttgcaagttt
    97081 gcgcccaatc ctttatcaac taatgctacc caagtattag ctgttgcgat tccgtttgat
    97141 tgttctgacc aatctgggaa gttgtctgca tataattcaa attgttcttg tagacctttt
    97201 accacgtcag tatctttgaa gaataatact gtaccataac catttttgaa accagctaat
    97261 ttgttttgtg tatttgggaa agcttctgct ggagtcaatg ggcgaagtgc ttcttcagtg
    97321 atttcccaaa gtttttcatg tgcatctccg aaaaggataa ctgcgcgtgt tgattgagca
    97381 ttgaaagcag ttgggctttc tttaattgct tctttgatca aagttgttaa ttcttcatta
    97441 gaaagtgaaa cattacgtcc tagatgatag attgaacggc ggtttttcaa agtgtcagta
    97501 aaagttgtca taataaaatt tctcctttga tttgcgtttt gcattttttt aattgattac
    97561 gaaatgaagt atagcacctt acaaaaagta agtaaacaga tatgctcaca aaaaataagc
    97621 gaagttcaaa aaacaatttt aataagaaaa atcgttatgt aaggattttc ttagttatcg
    97681 ctttcattgt tgtgttttag gaatttttgc ttatttgaaa aaaacagctg atatataatt
    97741 attaaatctc tatcttcttt tttttatgat attgccatta aaaattactt gcttttatag
    97801 aaaattagtt tatatttctt aaataagttt tttagttaaa tttttcggta aagatgtata
    97861 atattttctg aaaatttaaa gaattggggt atagattatg gaaaaacagc aagacaaggg
    97921 cttcttcggg cagccgaagg gattgtcaac tttgttcttc actgagatgt gggaacgatt
    97981 tagttattac ggtatgcgtg cattacttat ctattatatg tacgatacag ttgccaacgg
    98041 gggacttgga ttaccacgag caacagcatt agctatcatg tcgatctacg gatctttgat
    98101 ctacatgtct agtatcgtcg gcggatgggt atctgaccga gtgttaggag caaaacgcac
    98161 tgtattcttc ggtggagttt tcattatgct tggccacatc gtcttggctt taccatttgg
    98221 tgtaagtaca ttgtttgttt cgatggcatt gattattatc ggaaccgcct ttttgaaacc
    98281 aaatgtttct ggcatggtcg gacatcttta ttcaaagact gacttgcgaa gagatgcagg
    98341 attttcgatt ttttacatgg gtgtcaactt aggggcatta ttagcaccac taatcgttgg
    98401 tacaatcggt caagaatata actatcattt agggttctct ttagctgcta ttgggatgtt
    98461 ctttggatta ttacaataca tcatccaagg acgcaagaca ttagacggag tgggtacaac
    98521 accgccgaat cctttgacaa ctgaagaacg taaaggattc atccgtaact tgatcatcgc
    98581 acttgttgtt atcgctgcag tattcggcgg ggcacaacta actggtcatc tgacaattga
    98641 cttttttgtc aatacaatca gtgtattggg gattatctta ccgttgtatt actttatcaa
    98701 aatgctgact tcaaaagatg taactgctga agaaaaacca agagtatggg cttatatccc
    98761 attattcctt gcagcaatca ttttctggtc attacaagaa caaggttcat ctatcttagc
    98821 tttatttgct agtgaacgta cacaatctac gctatttggt gtaacgatcc aagcaagctg
    98881 gtatcaatca ttgaatccaa ttttcatctt tattctgaca ccagtattcg taacgatgtg
    98941 gacgaaactt ggtgaacgtc aaccatctac agtagtgaaa ttctctcttg gacttttatt
    99001 tgcaggatta tcgtacttgc tattgatgtt gccgggtatg ctttatggta caagtggaaa
    99061 agtaagtcca ttgtggttgt tgggaagttt cttcatcgtg gttattgctg aattgtgtct
    99121 ttcaccagtt ggtttgtctg tgacaacaaa attagcacca cgagcatttg aatcacaaac
    99181 aatcgctatt tggttcttag ctgatgcttc ttcacaagca atcaatgcac aaattgcacg
    99241 cttctacaca ccgggaacag aatctgctta cttcggtatt atcggtgcag tagcaatcgt
    99301 cggtggtatc ttgctattta ctgtgaaaga accaatcaaa aaactaatgg gctctattca
    99361 ttaatcaatg aacataaaaa caggaattgc ttatttgcag cagttcctgt tttt
//
LOCUS       DAFELO010000004        93470 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFELO010000004
VERSION     DAFELO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.004"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            85..972
                     /locus_tag="LP452_000302"
     CDS             85..972
                     /locus_tag="LP452_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2013063.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="LP452_000303"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="LP452_000303"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL2013064.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="LP452_000304"
     CDS             2274..2720
                     /locus_tag="LP452_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2013065.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="LP452_000305"
     CDS             2993..4345
                     /locus_tag="LP452_000305"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL2013066.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="LP452_000306"
     CDS             4516..4803
                     /locus_tag="LP452_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013067.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="LP452_000307"
     CDS             5029..5472
                     /locus_tag="LP452_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2013068.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="LP452_000308"
     CDS             complement(5478..6662)
                     /locus_tag="LP452_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2013069.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGCAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="LP452_000309"
     CDS             6930..7628
                     /locus_tag="LP452_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL2013070.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="LP452_000310"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="LP452_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL2013071.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="LP452_000311"
     CDS             complement(8438..8971)
                     /locus_tag="LP452_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013072.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="LP452_000312"
     CDS             complement(9268..11649)
                     /locus_tag="LP452_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL2013073.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LP452_000313"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LP452_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL2013074.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="LP452_000314"
     CDS             12350..12889
                     /locus_tag="LP452_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL2013075.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="LP452_000315"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="LP452_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL2013076.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="LP452_000316"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LP452_000316"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="LP452_000317"
     CDS             13996..15162
                     /locus_tag="LP452_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL2013077.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="LP452_000318"
     CDS             15208..16704
                     /locus_tag="LP452_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL2013078.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="LP452_000319"
     CDS             16785..17468
                     /locus_tag="LP452_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2013079.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="LP452_000320"
     CDS             18113..19303
                     /locus_tag="LP452_000320"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL2013080.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="LP452_000321"
     CDS             19405..20358
                     /locus_tag="LP452_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL2013081.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="LP452_000322"
     CDS             20427..21776
                     /locus_tag="LP452_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2013082.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVRKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVSAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="LP452_000323"
     CDS             complement(21959..22282)
                     /locus_tag="LP452_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013083.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="LP452_000324"
     CDS             22411..23574
                     /locus_tag="LP452_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2013084.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="LP452_000325"
     CDS             23644..24993
                     /locus_tag="LP452_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2013085.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="LP452_000326"
     CDS             complement(25038..25469)
                     /locus_tag="LP452_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2013086.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="LP452_000327"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="LP452_000327"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL2013087.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29308
                     /locus_tag="LP452_000328"
                     /pseudo
     CDS             28699..29308
                     /locus_tag="LP452_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
     gene            29430..30578
                     /locus_tag="LP452_000329"
     CDS             29430..30578
                     /locus_tag="LP452_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL2013088.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30616..31029)
                     /locus_tag="LP452_000330"
     CDS             complement(30616..31029)
                     /locus_tag="LP452_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL2013089.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31108..31539
                     /locus_tag="LP452_000331"
     CDS             31108..31539
                     /locus_tag="LP452_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL2013090.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31638..31871)
                     /locus_tag="LP452_000332"
     CDS             complement(31638..31871)
                     /locus_tag="LP452_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL2013091.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32054..33724
                     /locus_tag="LP452_000333"
     CDS             32054..33724
                     /locus_tag="LP452_000333"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL2013092.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33871..34257
                     /locus_tag="LP452_000334"
     CDS             33871..34257
                     /locus_tag="LP452_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013093.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34244..34942
                     /locus_tag="LP452_000335"
     CDS             34244..34942
                     /locus_tag="LP452_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2013094.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34947..35411
                     /locus_tag="LP452_000336"
     CDS             34947..35411
                     /locus_tag="LP452_000336"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL2013095.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35408..36319
                     /locus_tag="LP452_000337"
     CDS             35408..36319
                     /locus_tag="LP452_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2013096.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36554..37093
                     /gene="pyrR"
                     /locus_tag="LP452_000338"
     CDS             36554..37093
                     /gene="pyrR"
                     /locus_tag="LP452_000338"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL2013097.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37107..38390
                     /locus_tag="LP452_000339"
     CDS             37107..38390
                     /locus_tag="LP452_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL2013098.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38403..39329
                     /locus_tag="LP452_000340"
     CDS             38403..39329
                     /locus_tag="LP452_000340"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL2013099.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39348..40628
                     /locus_tag="LP452_000341"
     CDS             39348..40628
                     /locus_tag="LP452_000341"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL2013100.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40633..41709
                     /gene="carA"
                     /locus_tag="LP452_000342"
     CDS             40633..41709
                     /gene="carA"
                     /locus_tag="LP452_000342"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL2013101.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41714..44899
                     /gene="carB"
                     /locus_tag="LP452_000343"
     CDS             41714..44899
                     /gene="carB"
                     /locus_tag="LP452_000343"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL2013102.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44953..45729
                     /locus_tag="LP452_000344"
     CDS             44953..45729
                     /locus_tag="LP452_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL2013103.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45726..46658
                     /locus_tag="LP452_000345"
     CDS             45726..46658
                     /locus_tag="LP452_000345"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL2013104.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46655..47371
                     /gene="pyrF"
                     /locus_tag="LP452_000346"
     CDS             46655..47371
                     /gene="pyrF"
                     /locus_tag="LP452_000346"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL2013105.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47378..48007
                     /locus_tag="LP452_000347"
     CDS             47378..48007
                     /locus_tag="LP452_000347"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL2013106.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47995..48309
                     /locus_tag="LP452_000348"
     CDS             47995..48309
                     /locus_tag="LP452_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013107.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48347..48988
                     /locus_tag="LP452_000349"
     CDS             48347..48988
                     /locus_tag="LP452_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2013108.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49031..49723
                     /locus_tag="LP452_000350"
     CDS             49031..49723
                     /locus_tag="LP452_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL2013109.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49720..50622
                     /locus_tag="LP452_000351"
     CDS             49720..50622
                     /locus_tag="LP452_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2013110.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50780..52486)
                     /gene="efbA"
                     /locus_tag="LP452_000352"
     CDS             complement(50780..52486)
                     /gene="efbA"
                     /locus_tag="LP452_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL2013111.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52676..53299
                     /locus_tag="LP452_000353"
     CDS             52676..53299
                     /locus_tag="LP452_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013112.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53749..54798
                     /locus_tag="LP452_000354"
     CDS             53749..54798
                     /locus_tag="LP452_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2013113.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54795..55676
                     /locus_tag="LP452_000355"
     CDS             54795..55676
                     /locus_tag="LP452_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013114.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55679..56449
                     /locus_tag="LP452_000356"
     CDS             55679..56449
                     /locus_tag="LP452_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2013115.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56728..57582
                     /gene="aroE"
                     /locus_tag="LP452_000357"
     CDS             56728..57582
                     /gene="aroE"
                     /locus_tag="LP452_000357"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL2013116.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57760..58923
                     /locus_tag="LP452_000358"
     CDS             57760..58923
                     /locus_tag="LP452_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2013117.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59040..59450
                     /locus_tag="LP452_000359"
     CDS             59040..59450
                     /locus_tag="LP452_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2013118.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59624..61852
                     /gene="topB"
                     /locus_tag="LP452_000360"
     CDS             59624..61852
                     /gene="topB"
                     /locus_tag="LP452_000360"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2013119.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGNLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62023..62307
                     /locus_tag="LP452_000361"
     CDS             62023..62307
                     /locus_tag="LP452_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013120.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62467..62703
                     /locus_tag="LP452_000362"
     CDS             62467..62703
                     /locus_tag="LP452_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2013121.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62700..63098
                     /locus_tag="LP452_000363"
     CDS             62700..63098
                     /locus_tag="LP452_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL2013122.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63765..64709)
                     /locus_tag="LP452_000364"
     CDS             complement(63765..64709)
                     /locus_tag="LP452_000364"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL2013123.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(65005..65949)
                     /locus_tag="LP452_000365"
     CDS             complement(65005..65949)
                     /locus_tag="LP452_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013124.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66170..67384
                     /locus_tag="LP452_000366"
     CDS             66170..67384
                     /locus_tag="LP452_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2013125.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67406..69421
                     /locus_tag="LP452_000367"
     CDS             67406..69421
                     /locus_tag="LP452_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL2013126.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69504..70877)
                     /locus_tag="LP452_000368"
     CDS             complement(69504..70877)
                     /locus_tag="LP452_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL2013127.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70903..71187)
                     /locus_tag="LP452_000369"
     CDS             complement(70903..71187)
                     /locus_tag="LP452_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2013128.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71189..73204)
                     /locus_tag="LP452_000370"
     CDS             complement(71189..73204)
                     /locus_tag="LP452_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2013129.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73383..73688)
                     /locus_tag="LP452_000371"
     CDS             complement(73383..73688)
                     /locus_tag="LP452_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL2013130.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73777..74808)
                     /locus_tag="LP452_000372"
     CDS             complement(73777..74808)
                     /locus_tag="LP452_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2013131.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75015..75890
                     /locus_tag="LP452_000373"
     CDS             75015..75890
                     /locus_tag="LP452_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2013132.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76042..76884)
                     /locus_tag="LP452_000374"
     CDS             complement(76042..76884)
                     /locus_tag="LP452_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2013133.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76976..78853)
                     /locus_tag="LP452_000375"
     CDS             complement(76976..78853)
                     /locus_tag="LP452_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2013134.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78850..80271)
                     /locus_tag="LP452_000376"
     CDS             complement(78850..80271)
                     /locus_tag="LP452_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2013135.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80274..83663)
                     /locus_tag="LP452_000377"
     CDS             complement(80274..83663)
                     /locus_tag="LP452_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL2013136.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83920..85312
                     /locus_tag="LP452_000378"
                     /pseudo
     CDS             83920..85312
                     /locus_tag="LP452_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(85385..86032)
                     /locus_tag="LP452_000379"
     CDS             complement(85385..86032)
                     /locus_tag="LP452_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL2013137.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86040..86726)
                     /locus_tag="LP452_000380"
     CDS             complement(86040..86726)
                     /locus_tag="LP452_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013138.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86732..87805)
                     /locus_tag="LP452_000381"
     CDS             complement(86732..87805)
                     /locus_tag="LP452_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013139.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88027..88701
                     /locus_tag="LP452_000382"
     CDS             88027..88701
                     /locus_tag="LP452_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2013140.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88706..90046
                     /locus_tag="LP452_000383"
     CDS             88706..90046
                     /locus_tag="LP452_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2013141.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90195..91181)
                     /locus_tag="LP452_000384"
     CDS             complement(90195..91181)
                     /locus_tag="LP452_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL2013142.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91905..92936
                     /locus_tag="LP452_000385"
     CDS             91905..92936
                     /locus_tag="LP452_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2013143.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93045..>93470
                     /locus_tag="LP452_000386"
     CDS             93045..>93470
                     /locus_tag="LP452_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL2013144.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        29896 a        16580 c        18970 g        28024 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcacaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtccgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 gcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctgggttc cttggctggg
    28801 gtttattcca ttaggattca ttagtttaac aattatccat atcacagtaa ttattgctgc
    28861 agtagtatta ggcccaaaag atggcatggt gattggcttg ttttggggaa tcgctacaat
    28921 tgttcgtgcc tatgccatgc caacaacgcc atttgataca ttagtgttca cgaatccgat
    28981 tatctcggta gttcccagag tacttgttgg cttagtagca ggcttggttt tccactggat
    29041 ttatcaaaaa aataaatcaa taacgatcag ctcagccttt gcaggggtac tgggttcatt
    29101 ggtcaataca gtcttagtgt taggattcat gggattattt tatacaggtg caacggcaga
    29161 tgcctatggt gtagactctg ctcttttatt taaaacctta gcaggggttg cagcaatcaa
    29221 tgggatacca gaagcaatag gtgcgggaat cataacacca ttactagcaa aagcgttatt
    29281 tgcagcaaca ccgttgaaac cagaataatg accatctttt aatagtaata ggacgcacga
    29341 tagatagaaa aaggaagaga ggagaggaaa acaatcgtcc tctccatctt tttacctgag
    29401 caacaagcaa actagaaggg gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac
    29461 aaataccgca acagcggaca aaaaaaggag tactagcggg tatcttagga ttggttacga
    29521 atctgatctt gtttgtctct aagctaatga tcgggctttt ttcaggaagt gtctccatta
    29581 tggctgatgc agtgaacagt ctatcagata ctgcttcatc catattgact ttgatcggat
    29641 ttaaaattgc tgcaaagcca gcagatcagg aacatcctta tggacacgaa cgcttcgaat
    29701 atatcagtgg gttatttgtt tcgatcatca ttacttatgt aggatttcaa tttttagagt
    29761 cttctgctga aaaaatcatc catccagaaa atgtctcttt aacacccatc gtcttttttg
    29821 tcctaatcat ttccatcttt ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc
    29881 aatccattga ttctgaaaca ttgagagcta cagcaacaga tagttataat gatgtcttta
    29941 ctacactggc tgtattgctt tcagcagcaa tcgaatgggt aactggatgg cgtattgatg
    30001 gatatatagg ttttgcttta gcattatata tcctttatag tggattgacg atgcttcgaa
    30061 attttgtata cgaactatta ggaagtcgcc caacgccagc agagattgaa gaaatggaag
    30121 agcatttaaa tgcttgcgat gatatacttg gctatcatga tttattggtg cataactatg
    30181 gacccaataa acgctttgca tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg
    30241 tggctcataa gattattgat ctgatagaaa aagactttaa aaagtctttg gatgtggaac
    30301 ttgtctgtca cttggatcca atgcctgttg ataacgaaaa atactataat atcttgcggc
    30361 agttaaagca aattcttctt gaaacaggta cagggcttaa tattcatgat tttagagtaa
    30421 ttcatcaagg aaaaacgctg caatttgatg tagtggtccc tgagaaattc cagtttgttg
    30481 atacagaact agaaagaatc ttacgtagtc aaatagaaga aaaaatcgga gattaccaat
    30541 tggaaattac gtttgatcac aattatcttt tatattaaat gagaaactat aaaaagactc
    30601 cttaaagttt ttaatttaaa gagtcttttt ttcttttaat tgtttatgga gagcctgacg
    30661 tgcaagatta tctgctcctt tatttttgtt ttcgggcaac cattgcagga tcaataaaga
    30721 aaattcggat agaagttctt ggattctttg aaggtaggga aggaagagct cgttcttcgt
    30781 tgcgtttttg tcgaccgtag aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc
    30841 attctgtaag tcgtttttct ttagaaattc taaagttttt aaaaagactt cgaactctgc
    30901 ttgatggttt gtccctatag aaagcgggat agatagctgt tcttgtttac cttgatagac
    30961 aatcaagatt ccacctccac taggaccagg gttgcctttt gtagaggcat caatgtatac
    31021 ttttaacata aaaagagatt ctcctttttg ttcaaactaa tgttatggta agattatatc
    31081 attactgaat cggtgagagg ttagagaatg aaaaaaaata ttcgatggca gcctgaactg
    31141 gcacatacga tcatctattg gtcattgacc ttgatcattc tgttttttag tctgattctt
    31201 tctttagaaa acacaggtcc ttattggaaa agcaattttg ttttaggtat ttttcttgtt
    31261 tttctatttc taggtttgcg gcgttctttt attttaaaat caaactctgt gattatacgt
    31321 tatgctgctt tttggaaaga ccgagaaata tctgtcggac agattcgatc tttgacgcca
    31381 actaatactg gggtgcaaat gatgttgcaa gatgagaaag ccccatctgt tttcatgatg
    31441 cgaaaaaaag atcaagtagt cctttttgat cattttgaaa aaaacggacc caaatcagaa
    31501 tcaattcctt atccttatac gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa
    31561 aaaacgaatt tttacaggaa aagaaaagag tgagacgtac ggttcatccc gtttagcgtc
    31621 tcactctatt tgtttatcta ttcatgcata ctagtatcgg tagtctgaac gttgcctttc
    31681 ttgacatggg cagcttgtgt tcctcgattc ccttcgatga tctcaaattc gaccacttga
    31741 ttttcttcca aagttttgaa tccctcttct tcaatcgcag taaaatgtac aaaaatttct
    31801 tctgtgtcat cataactgat aaagccgtat ccttttttgt tgtcaaacca ttttacaatt
    31861 cctgtggtca aagcactcac gacctttcta aagtctgatc atagccttca ttataaagag
    31921 tatttataag tacgtcaaat aaattaaaaa cctgtaattt tatgtttttt ttctgaataa
    31981 aaaaaggtat aattggcata tcttaacaaa gaagatattt tgttgaataa agttcgtgaa
    32041 aaggggtaat caaatgaaga atgatctgca aatttcacaa gaaacagagc tagaaccgat
    32101 tgttgaagtg gctagtaaat tagatcttaa ggaagacgat ttggaactgt atgggaaata
    32161 taaggcgaaa atcaattttt ctggaatcaa tcgatcaaag gataacgctg aggggcattt
    32221 gatcttagtt acttccatca atccaacacc agctggcgaa ggaaagtcaa cgatcactgt
    32281 agggttagga gacgcgctca ataaaatcaa taaaaaatct gttattgcat tgcgagaacc
    32341 ctcacttggt cctgtgatgg gaatcaaagg tggtgctgca ggcggaggat atgcacaggt
    32401 attgcctatg gaagatatca acttgcattt tactggtgac atgcatgcta tcacaacagc
    32461 taataatgca ttatctgctc ttttagataa tcatattcat caaggaaatg aattgaacat
    32521 cgatgcccgc cgagtgattt ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa
    32581 agtaattgtt ggcttgggtg ggccaatcca aggtgttcca agagaagatg ggtttgatat
    32641 cacagttgcc agcgaaatca tggctatctt gtgtcttgcg gcagaccttg aagatttaaa
    32701 ggctcgtctt gcaaggatcg ttgtcggcta tacatttgac cgtcagcctg tgactgctgg
    32761 agacttgaaa gcagaaggcg ctctggcatt actgctgaaa gatgcaatca agccgaatct
    32821 tgtacaaaca atttacggaa caccagcttt tgtccacggg ggaccgtttg ccaatatcgc
    32881 tcatggctgc aatagtattt tagctaccaa gacagcattg agattagcag attacgttgt
    32941 cacagaggca ggatttggtg cagatcttgg cggagagaaa tttttagata tcaaagtacc
    33001 taacttgaaa aaagcgccgg atgctgtggt gattgtcgcc actgtccgtg cattgaaaat
    33061 gcatggcgga atgaaaaaag atgaacttaa aaatgagaat cttgatgctt tgaaagtagg
    33121 ttttgcaaat ttaaagcgcc atatacgtaa tatggaacaa tatcagctac ctgtgatcgt
    33181 cgcaatcaat gaattcgtaa cagatacaga caatgaactc acactgttgg agcacttgtg
    33241 tgaaaatcag gggattcttg ccaagagagc gagtgtatgg gcagatggag cagaaggcgg
    33301 cgttgattta gcaaaagctg tagtacgatt gattgataga aaagaagcta attacaagcc
    33361 attataccgc ctagaagaaa cgatacaaga aaagacagaa acaatcgtca agaaaattta
    33421 cggaggaaat ggtgttgttt tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa
    33481 gaatggatgg gacaaactgc cgatttgtat ggcaaaaaca cagtattcat tttctgatga
    33541 tccttcactg ctaggtgcac cggaagattt caccatcacg atccgcgaaa tcatcccaaa
    33601 attaggggca ggatttttag tagccttgac tggagatgtt atgacgatgc ctggtttgcc
    33661 taaaaaacct gctgcattga atatggacgt gacaaatgac ggagaagtgt tgggattgtt
    33721 ttaagcttga ttataatcta ccatcgaagg agctgtattt tgactacagc ttcttttttt
    33781 agacgatagg gaattaattt tgtttacact aggatcattc tttttcttta ttgtattttg
    33841 agctatacta aagcataata ggagggtatg atgaaaacga catttataag aaaaaccggt
    33901 ttttacggta tgggtagctc gattcagcta agaatcaaca atgaaaaacg cagtgttctc
    33961 agtcatgaac aaaagataac gcttgatctg gaagtgccct ttacgatgca ggtcaccttt
    34021 tattggctaa aaagccccgt ttatacagtg aaagaagcaa agcctgctta tgtgatcaca
    34081 atgaattact tgattcttca aatctatccg tttttatttc tagcaactgg gttgggggcc
    34141 gttttcctac aaaatttatt tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt
    34201 ttctatataa aaaaccgggc atatttgatc aaggaggaaa attatggcaa actctgatct
    34261 ttttttaatc agtttcaatc gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg
    34321 aaatatggga tttaccgaac ttgtcagacg cctagctcaa agaaaacaat taatgatcag
    34381 aaaatacgaa gatgatcttt tacagcttgc tcagttaagg aatgcgatcg tccatgatcg
    34441 gatcgcagtc gattttgtca ttgcagagcc aaatgattgg acagtcaagc gaatccagct
    34501 gatcgaacaa gaactgattc agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac
    34561 aggttttgaa caagatcttc cgttacttga actgttaaag atagtagcag aaaaaaggta
    34621 ttcacaattt ccgttataca ataaaggaaa atttgtagca ttgattactt tacgtaatat
    34681 cgggttttgg ctagcaaaag aaagccaaaa aggtccggtt gatttaacaa ataaacgagc
    34741 gttggatttg atcatccaaa acggaaagta cacaaattat cattttgttc cagctaaaac
    34801 acatatttac gaggttgaag cgatgtttcg tgagcaaggg actttggaag ccgtacttat
    34861 cactaaagat gggaatcccg atggtaatct gctcgggatc gtacgacctc gtgatattta
    34921 taaacaaata gaaaaggatt gattttttgt taattattta ttggatattc agcgctttgc
    34981 tagtaggagt ggatcagttg gtcaaatggt ggatcactga taatctagcg cttggagaga
    35041 cgaaaaatct tataccaggt attttgtcac tgaatcatat tcgaaacact ggagccgcat
    35101 ggagtatgct agaagggaag atgtggttct ttactatagt gacactcatt gcagtcgttg
    35161 tgattctcac attgatgatt aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat
    35221 tgagtttgat tctagctggt gcgattggca attttatcga ccgtattcgt cttggctatg
    35281 tagtagatat gttccagaca gattttatga gctttccgat ttttaacgta gcagatgtga
    35341 cacttgtatg tggagtgatt tgtatgttaa tttatattat tttagatgaa aaggatcaaa
    35401 gaaaaaaatg acagaactta aaacgacgat tcaccatgaa tctggtagga tcgataaagt
    35461 attgactaat ttattcaccg attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg
    35521 agcggtttct gtcaatggac agccagtcaa agcaaattac aaagtaaaac aaggagacga
    35581 gattactgtc ttgatccccg aaccagaaac attagatgtg gtcccagaaa atatcccgtt
    35641 gacgatcgtt tacgaagatg aagatgtagc ggtgataaac aaacctcaag gaatggtcgt
    35701 tcatccttca gcaggtcata cgcatggcac aatggtcaat gcgctgatgt accatatcaa
    35761 ggatctttct acgatcaacg gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga
    35821 tacttccgga ctgctaatgg ttgccaaaaa tgaccaggca cacgaatcat tagctcagca
    35881 attaaaagat aagacctctt tgcgcaaata tgttgcttta gtccatggag agattcctca
    35941 cgaaaaagga accatcaatg cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc
    36001 gataagagaa gatgggaagt cggctgtcac acattttact gtattggaac gtttcgaagg
    36061 atttactctg gttgaattaa aactagaaac cggaagaaca caccagatcc gtgtgcatat
    36121 gaagtatatc ggttatccgt tagctggaga tccagtatac ggaccgaaga aaactttgaa
    36181 aggcaatggt cagtttctcc atgcgcaaat gttaggtttt acgcacccga ctacaaacga
    36241 aaagatgatc tttgaagcac ctcttccaga agtttttgaa aaaactttag aaaaattaag
    36301 aaaaaacatt gcattttaat ttagataacg gtatagtaat taacaacgaa agcataaaac
    36361 ttaatagaat tcctttaagt ttagtcccgt gaggctaaga agggtgggag caaggatacg
    36421 tatggtgcgc cataagtaga ttgctcattg acatagtata gctatcagtg tatcagtata
    36481 cgctcagtct aaacattcct cctgtcctct tagggcagga gtttttttat gcaaaaaagt
    36541 agggggaatg aaaatgcaag caaaagaagt agtagatcag gtaaccatga agcgagcatt
    36601 gactcgaatt acttatgaga ttatcgagag aaaccatagt atccaagata tcgtattggt
    36661 aggtatcaaa acacgaggaa tctacattgc atcaagaatt gccgaacgcc tgaagcagct
    36721 agaagatatc gatattcctg taggagaact ggatatcact ttataccgag atgacaaaaa
    36781 agaaaatcca gaagaaccgg aacttcattc ttctgacatc ccagtttcct tagaaggaaa
    36841 agaagttatt ttgattgatg atgttttgta tacaggacga acgattcgag cagcaatgga
    36901 cgcagtaatg gattttggca gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg
    36961 gcatcgagaa ttgccaatcc gtgcagatta tgttgggaag aacattccaa catctcgggc
    37021 ggaagagatt ttagtagaga tgcaagagct tgatggacaa gatcgcatca tgattttgaa
    37081 agaggaagat tagaggagag agaaacatgt cggagaaaaa aggatttcat aatgatgacg
    37141 tggtgttaga tatccacgat cgcccaaccc caggacattg ggtaggatta agtttacaac
    37201 acttgttcac gatgttcgga gcaaccgtgt tagtccccat cttagtaggt atcgatccag
    37261 gtattgcgct agtcagttca ggactaggta cattggtgta cttatttact actaaaggaa
    37321 agatacctgc gtacttagga agcagcttcg ctttcattgc agcgatgcag cttttgatga
    37381 aaacagatgg ttatccagcg attgcacaag gagcagtcac aactggatta gtctatttga
    37441 ttgttgcgct gatcatcaaa aagatcggtt cagcatggct ggataaaatc ttgcctccaa
    37501 tcgtcgtcgg tccagtcatc atggttatcg ggttgggact tgcggcaaat gcagcaacaa
    37561 atgctatgta taatcagggc acgtatgatt tcaagtatat tgctgtagcg ctgatcacat
    37621 taggattaac aattttcttc aacatgtggc taagaggttt tctcggattg attccgattt
    37681 tactaggtat tattagcgga tacatcgtag ccttgctgtt tggaatcgtg gatactaagc
    37741 caatcgtaga cgctgcctgg tttgctgttc ctaactttga gattcccttt gtacaatatg
    37801 aaccaaagct atatcttgga gcaatcacaa caatggcacc catcgctttt gtgacgatga
    37861 cagagcatat cggccatctt atggtattga ataaactaac taaacgtaat ttctttgaag
    37921 aaccgggtct tcataagacg ttgacaggtg atggattagc acaaatcgtg gcagcctttg
    37981 tcggaggtcc tcctgtaaca agttacggtg aaaacattgg tgttctggct atcacacgtg
    38041 tccatagcgt gtttgtgatc ggtggagctg cagtatttgc agtcatttta ggattcgtag
    38101 gaaaattaag cgctttgatt ttaagtatcc ctggaccagt catctcagga atcagtttta
    38161 tcttatttgg agtaattgcc gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt
    38221 ttgataaaaa gaaaaatctg ttgattgctt cagttatctt agtagtagga attggcggac
    38281 tcgtctttga agtaggcaca tttacattgt cggcaatggc attagcaact gtacttggca
    38341 tcattttaaa tcttatctta ccagaaactt cacgaagcga agaaaactaa ggaggaaaca
    38401 ttatgatcat caaatcagaa cgtatcagct taaagcattt attgaccgtt gaggcactta
    38461 ctgatcaaga agttatgggc ttgatccgca gagggcagga attcaagcaa ggtgctaatt
    38521 ggactccgca aaagcgccag tactttgcta caaacctctt tttcgaaaat agtactcgca
    38581 cacataaaag cttcgatgta gcggagaaaa aactagggct agaagtgatt gaatttgaag
    38641 caagcacaag ctctgtccaa aaaggggaaa cactatacga tacagtactg acgatgtcag
    38701 cattaggcgt agacgtagcg gttatccgtc atggtgatga gaactattat gacgaactga
    38761 tacaaagtaa aacaatccag tgttcgatta tcaatggagg agacggaagc ggacagcatc
    38821 caacccaatg cttgcttgat ttgatgacca tttatgaaga atttggtcat tttgaaggtc
    38881 tgaaagttgc catcgtaggt gatatcactc actctcgcgt agctaaatcc aatatgcaga
    38941 tgttaaaacg tctaggcgca caagtattct tctcaggtcc tagagaatgg tacgacgatg
    39001 aattcgaagt gtacggacat tatatgccat tggatgaact actagatcaa gttgatgtca
    39061 tgatgatgct tcgggtgcaa cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag
    39121 gctaccatca ggaacatggg ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa
    39181 tcattatgca cccagcacca gttaaccgtg atgtagaatt agctgattca ttagtggaag
    39241 gattacagtc gcggatcgtt cagcaaatga gcaatggagt attcgtcaga atggcaatct
    39301 tagaagcagt gctaaatgga aaagcgtaat tataggagga tgaaaaaatg aaaacactca
    39361 ttaaaaacgg tcagatcaat acacgaaaaa atatgacgac accagcagaa atttggatag
    39421 aagatgggcg aatcaaagca atcggtacag gattctcaga agccgaattt gatgaagtgt
    39481 ttgatgcaaa gggacaattg atcacacctg gtttagtgga cgtccatgtt catttgagag
    39541 agccaggatt tacatacaag gagacgatcg aagctgggac tcgctcagct gctcgtggcg
    39601 gtttcactac tgtatgcgcg atgccgaatt taaatcctgt acctgatacg gcagaaaagc
    39661 tgagacaagt atatgacatc attagaaaag atgcagttgt gaaggtattg cagtatgcgc
    39721 caatcacaga aaatctgcga agtgagaaat tagttgatca agaagcactg attgaagaag
    39781 gtgcttttgc tttcacaaat gacggtgtag gtgtccaaac agctgggacg atgtatctgg
    39841 ctatgaaaga agctgcaaaa aacaataaag ccctagtagc ccatacagaa gatgaatctt
    39901 tattatttgg cggcgttatg cacgctggca agaaggcaga agaacttggt cttcccggga
    39961 tcttaagcgt aaccgagtct tctcaaattg ctagagatct gttgctagct gaagcaactg
    40021 gtgttcatta ccatgtttgc catgtttcta ctaaagaaag tgtccgtgta atcagagacg
    40081 caaaaaaagc aggaatccac gtaacagcag aagtttcacc ccatcatctt attttgatcg
    40141 acgaagatat tcccgaagat ttcggctttt ggaaaatgaa cccgccgcta agaggacggg
    40201 aggacagaga agctttgatc gaaggcttgc ttgacggtac gatcgattgc atcgctacag
    40261 accatgcccc ccatggatta gaagaaaaaa gccaaagttt catgaaatca ccatttggta
    40321 tcgtaggaag cgaaacagcg tttcaactga tctatactca ttttgtagaa actggtcgat
    40381 tcacgttgga acaagtgatc aattggttgg cagttaaacc ggctgagatt tttggtttga
    40441 atgcaggaac gctaacggtt ggtgcccccg ctgatgtagc agtttttgat attacacaaa
    40501 cttgcacgat cgataaagaa gactttttat caaaagggga gaatacacct tttatcggct
    40561 ggaaagtaaa aggcgaaaca caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg
    40621 gagaataaaa gtatggaacg tttgcttata ttagaagatg gaacagtttt cgaaggaaag
    40681 gcttttggtg cagatgctag tgtcttcgga gaaatcgttt ttacaacaag tatgactggt
    40741 tatcaagaaa ctatcactga tcaaagtttt aacggtcaaa tcattacatt tacctatcct
    40801 atggtgggta attatggggt aaatagagat gattatgaat cgatttctcc aacatgcaaa
    40861 ggagtagtag taaaagaaca tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta
    40921 gattcatttt tgaaacaaaa aggaattcct ggaatatcag gtatcgatac gcgcgccctt
    40981 actcgcaaaa tccgtgaagt cggaacgatg aaagctagta tagttgatgc tggagattcc
    41041 ttcgaacatg catttgatca gctgaaagca accgttctag caaccaatca agtgcagcaa
    41101 gtctcaacta acaaacctta tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac
    41161 ttcggcttga aacatagtat tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta
    41221 cttccctata acacagatgc cgagacgatt ttagcgctat caccagacgg agtcatgctg
    41281 acgaatggac ctggtgatcc aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc
    41341 caaggaaaag taccgatttt tggtatttgt ctcggtcacc agttatttgc tttagccaac
    41401 ggtgcagata cctataaaat gaaattcgga catcgaggat taaatcaccc ggtacgagaa
    41461 gtagcgaccg gaagaattga ttttacttct caaaatcatg gctatgcagt cgatgaagcc
    41521 acgattgatc cagaaaaact catcgtaact catgtagaag taaatgacgg aacagtagaa
    41581 ggagtcaaac acagagatta tcccgctttc agtgtgcagt ttcatccaga tgctgcccct
    41641 ggtccgcatg atgcactaca tttatttgat gaatttatgg aaatgatgga cgcaggaaag
    41701 gagcagtaaa cagatgccga aacgtacaga tatcaagaaa attatggtga ttggatctgg
    41761 tccgatcatc attggtcaag ccgcagaatt tgactacgca ggaacacaag cctgccttgc
    41821 cttaaaagaa gaaggatatg aagtggtttt agtaaattct aatcctgcga cgatcatgac
    41881 ggataaagaa gtggcagatc aagtatatat cgaaccgatc acacttgaat ttgtatcaag
    41941 gattcttcga aaagagcgtc cagatgctct ccttccaact ttaggcggac agacaggcct
    42001 caatatggca atggagctgg cagcatcagg tattttagaa gaattgaaca tagaacttct
    42061 aggaacaaag ctttctgcga tcgatcaagc agaagatcgt gatctattca aacagttgat
    42121 gaaagaattg aatcagccaa tccccgagtc tgaaatcgtc aatacagtag aagaagctgt
    42181 agattttgca aataaaatcg gctatcctgt catcgttcgt ccagccttta ctttaggcgg
    42241 aactggtgga ggtatgtgtg aaaatgaaga agaattacgt atcgtatcag aaaatggatt
    42301 aagtctttcc cccgctaccc aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat
    42361 tgaatatgaa gtgatgagag attcggctga taatgcaatc gttgtctgca atatggaaaa
    42421 ttttgacccg gtgggtatcc atacaggtga ttctatcgta tttgccccta gtcaaacatt
    42481 gtctgaccat gaatatcaaa tgcttcgtga tgcttctctt tcgattattc gagcacttaa
    42541 gatcgaggga ggctgtaatg tccaactcgc tcttgatcca catagtttta attattatgt
    42601 gattgaagtc aatccgcgtg tttctcgttc atctgcgtta gcaagtaagg cgactggcta
    42661 tccgatcgct aagctagctg ctaaaatagc gcttggatta acattagacg aaatgaaaaa
    42721 tcctgttaca ggaacgactt ttgcagaatt tgaacctgca ttagattatg ttgtagctaa
    42781 aattcctcgc tggccgtttg ataagtttga aaacggagaa cggcttttag gcacgcagat
    42841 gaaagcaact ggtgaagtga tggctatcgg tcggaatatc gaagagtctt tattgaaagc
    42901 tgtgcgttct ttagaaatcg gtacacacca tatcgaactt cctgagttgg cagacatcac
    42961 ggacgatgac ctaatccaaa aagtggttcg agcacaagat gatcgcctgt tttatgtagc
    43021 tgaagctatt agaagagggt acaccataga agaattagcg gatttgacaa aaatcgattt
    43081 attcttttta gataaactgt tgcatatcgt agaaatcgaa cagcagttga aacagcagcc
    43141 gttggatgac aagatattga aaacagcgaa acaaaacggt tttacagaca aaaaagtggc
    43201 ggagctgtgg gaaataaacg aagaagccgt tcgccagatg agaaaagaaa atgatttgat
    43261 tcctgtatat aagatggttg acacttgtgc agcggaattc gaatcaaaaa caccttattt
    43321 ttatagttcc tatgaatatg aaaatgaaag ttcacgatca gaaaagccct caattctagt
    43381 actgggatct ggtcctatcc gtatcggaca aggggttgaa tttgactatg cgactgtgca
    43441 ttctgtcaaa gcaatccagc aggcaggata cgaagcaatc atcatgaaca gcaatcctga
    43501 aacagtttcg actgactttt ctatttcgga taaattatat tttgagccat tgaccttcga
    43561 agatgtgatg aatgtgatcg agctagaaca gccgatagga gtaatcgttc aatttggtgg
    43621 tcagacagca atcaacttag ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac
    43681 aacgatcgaa gacttggatc gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga
    43741 gttggatatt cctcaacctt taggggatac tgctacaagt aaagaagaag ccgtggttat
    43801 cgcttcgaga attggttatc cagttcttgt tcgtccaagc tatgtattag gcggacgtgc
    43861 gatggaaatc gttgaaaacc agcgagattt agaagattac atggaacatg ctgtgaaagc
    43921 atctccagaa catccagtac tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga
    43981 tgcgatttgt gacggtgaaa cagtactcat cccagggatc atggagcata ttgagcgtgc
    44041 tggtgtccat tcaggggatt caatggctgt gtatccacct caaacattat ctgaatcgat
    44101 caagaaaacg atcgaagatt acacgatccg cttagcatta ggtttgaatt gtgtcgggat
    44161 gatgaatatc cagtttgtca tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg
    44221 agcaagtcgg actgttccat tcttaagtaa ggtgacagga attccaatgg cacaaattgc
    44281 gacgaaagct attttgggag agaaactaaa gacacttggt tatgaaaacg gactttatcc
    44341 agaaacaaaa gaagttcatg ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt
    44401 tgatacttac ttagggcctg aaatgaaatc gactggagaa gtaatgggat ctgacagaca
    44461 attagacaaa gctttgtaca aggcattcga agcttctgga ttacacttgc ctgaatatgg
    44521 agccgtatta tttacgattg cggacgaaac aaaagaagaa gcactagaat tagctaaacg
    44581 ttttacagaa attggctata gtctgatagg aacgaaacat acagctgctt atttcgaaaa
    44641 aaatggtctg gtcgttgaaa cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt
    44701 agtcgactta ataagagatg gtaaaacaca agtggttgtc aatacgattg ataaagatcg
    44761 gggaaatgct tctaaagacg gtttcatcat ccgaagagaa gcagttgaac atggtattcc
    44821 attgtttact tctttagaca cagcagatgc gatcgttcga gtgatggaat cccgtgcttt
    44881 ttcaactaac cctatttaac ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc
    44941 aggaggggac agatgaaaca agaaatgatg gaaatcgttc agcaaaaaca gctggcacca
    45001 aagatttttg aaatgacatt gaaaggtgat ctagtaaaag aaatgcagca accaggacag
    45061 ttcttgcata ttctcgtgcc gcgtgcagac ttgctattac gccgaccgat tagcttgaat
    45121 catatcgacc acgaaacaaa tacttgccgt atcatttatc gggtggaagg tgaggggact
    45181 agagtcttct ctactctttc tgccggagac catttagatg tcatgggtcc tttaggcaat
    45241 ggttttgatt tgtccaatct tcatacagga gacgaagtat ttataatcgg tggaggaatc
    45301 ggtgtccctc ctctatatga gttatcaaaa cagttagtcg aaaaaggaat taagccaacg
    45361 cattttttag gttttgccac acatgaagtc gtttattatg aaaaagaatt taatcgtcta
    45421 ggagaaacaa gaatcgctac tgacgatgga acatacggta tgcacggaaa tgtcggtaat
    45481 ttattgttag gaacgaagac ggaacctaca gctgtttttg catgtggttc aaacgggtta
    45541 ttgaagacag tggaacagct gttttcttcg catcaaaatg tccagttatc attagaatcc
    45601 cgcatggcat gtgggatagg ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa
    45661 acaggaacaa aaagtgtgaa agtttgtgac gaaggaccga tttttaaaat aggagaggta
    45721 attatatgaa tgagaatcct ttagcggtca aacttcctgg cctagaatta aaaaatccta
    45781 tcatgccggc aagcggctgt ttcggttttg gcaaagaata cggaaaatac tatgatttga
    45841 atcagcttgg atcgatcatg gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca
    45901 cgccaagagt ggcggaaaca ccatctggca tgttgaatgc aattggtttg caaaatcctg
    45961 gtctagacgt tgttatgcat aaacttcttc cagaattaga ggagtacaat gagttaccca
    46021 tcatcgcaaa tgttgctggt gcttgtgaag aagactatgt agaagtttgt agcaaaattg
    46081 gcgaagcacc aaatgtgaaa gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg
    46141 gcggaattgc ttttggaaca gattctgatg ttgcttttca attaacacaa gcagtaaaga
    46201 aagtttcttc tgttcctgtc tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa
    46261 tcgctcaggc aatcgaagct ggaggggcag atgggtttac gatgatcaat actcttcttg
    46321 gtatgcggat tgaccttaag acaagaaaac cgattttagc caatcaaaca ggtggactct
    46381 ccggaccagc aatcaagcct gtcgctattc gtttgattca ccaagtagca agtatatcca
    46441 gccttccaat cattgggatg ggcggcgtgc agacagtgga tgatgttctt gagatgttta
    46501 tggctggagc aagtgctgta gctataggga ccgcgaattt caccgatccg tatatctgtc
    46561 ctaaactaat taaagagctc cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa
    46621 gattaatcaa agaagtgaga gaggaacgtg aacgatgaat caactagacc aacgaccaat
    46681 tattgcatta gatttttcaa ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa
    46741 agaagaaaaa ttgtttgtga aaatcggaat ggaattgttc taccaagaag gacctgaaat
    46801 cgtacgttat ctaaaagagg ctggtcataa tgtattttta gatctgaaac ttcacgatat
    46861 tccgaatacg gtcgaaaaag caatgcgggg attggcaaag cttggtgttg atctcacctg
    46921 tgttcatgcc gctggcggta ttcgtatgat ggaggcggca atgcgaggat tagaagaggg
    46981 gactccagaa ggtggaaaac gtccgctact tctagctatc actcagctta cttctacaag
    47041 tgaagaagaa atgcatgccg atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca
    47101 ttatgctagc tgtgccaaaa aagcaggatt agatggtgtg gtttcttctg cttgggaagt
    47161 agaagcgatc aaagaaatgg caggagacga atttgtttgc ttaactccgg gtataagacc
    47221 agaaggaaca gtcgcaggag atcagacaag agtagtcaca ccttctcagg caaaaaagat
    47281 cggctcaact tttatcgtta taggtcgacc aatcactcag tcaactaatc cttataaagc
    47341 atatcaaacg attcaaacag aatggagcta acctaaaatg aatgttgaac aaagtatcgc
    47401 aaaagactta ttagaaattg aagcagtatt tttaaatcca agtaatcctt ttacatgggc
    47461 aagtggaatc aaaagcccaa tttactgtga taaccgaatc acgatgagct atccaaaagt
    47521 ccgaaaagag atcgcaaaag gattagcaag caaaatcaaa gaagcttacc ctgaagtaca
    47581 agtgattgct ggaactgcaa cagcaggaat ccctcatgcc gcatgggtag cggaaatact
    47641 ggatttgcca atggtctata ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat
    47701 cgaaggacgt atatttgaag gtcagaagat ggttgtgatc gaggatttga tttctacagg
    47761 cggaagtgtc ttggaagcag ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt
    47821 tgcggctatc tttacgtatg agttgccaaa aggaaaagca aatttcgaaa aagccgaaat
    47881 ccctcttatg acgttgacca attattctgt attgattgaa gcagctttag aagatcgtta
    47941 tatcgatgag caagaattaa ctctgctaaa agaatggaaa aaagatcctg aaaattggca
    48001 gatttaagcg cgcttgatga agatagagaa atccgatttg tgtctccaga atcatcaatt
    48061 ggaatagcaa aaatccaaaa actgattgcg agaagcatct tttcatcagg tatgaaaaga
    48121 aacttctatc gtgatcagtt tttgattttt ttaaaattca tttttctaag actggttagc
    48181 agaaagaaga gaataatctc ttctcttttt tctattatat atagattttc tctatttttt
    48241 ggtaagggag ctgtttcaaa aatggtaaag agcggaaatg ttgcgaatat cagttattta
    48301 atgatatgat attcggaaaa agaaatctca aagggaggta acttttatgg ggttgaatga
    48361 atttatttta ggattgaaca atttagagac gatcacaagg gcacctggat tttttaaata
    48421 tacagagcat acagttgcag cacattcgta tcgtgtagca tctattgcgc aagttctagg
    48481 agacattgaa gaagcgaatg gacaaccgat cgattggaaa ctgctctatg aaaaatcatt
    48541 gaatcatgac tacacagagc gttttattgg tgatatcaaa acgccggtga aatacgcaag
    48601 caaagagtta agagggatgc tgcaaacagt tgaagaaaag atgacggacg aattcatcaa
    48661 ccaagagatt ccagctgagt tccaagaagt ttatcgccgt cggttgttcg aaggaaaaga
    48721 tgaaaccgta gaaggagaac tattagccat ttcagataag gttgaccttc tttatgaatc
    48781 ttttgatgaa atcatcaaaa ataacccgga aaaagtttat aaggagatgt tcattgaggc
    48841 agtgactacg attaaaagct ttgaacatcg gccttctgtt cagtatttct ttaaagagat
    48901 atttccggaa ctattgaacc aggaatttta tggaaaagag gcgtttttag aaaccgtctc
    48961 aacgattgta aagaaagaag aaaaataaaa atgatatgag atgcaggggg aagtatagtt
    49021 gaaaaagaat atggatgtcg aatggggcta ttctggagac aaaggtccgg aaaattgggc
    49081 gagtttatgt gattggtttg cgagaggtgc ggaatttcca ttgcagtcgc ctattcattt
    49141 gaccaagggg gaagaaacca aaatagaagg agacacgctt tcctttcatt atcaaaagca
    49201 acggtttact gacaaggaat tcaaaaacac gattcattta gtaccttttg accaactcag
    49261 ttatgtagag ttcaagaaag aacgctatta tttgacagat attcatttcc acttgccttc
    49321 tgaacatatc atcaacggaa aacaggaaga tattgaattt cattttgttc atatgaacag
    49381 caaaaaagag aatctagttg tgggtgtcat gtaccagcta atggtacagg aaggttggct
    49441 gtataatcaa gaaaatgggg aaagttggaa cttagaaaaa catgagcatt gggtaaatcc
    49501 ggatgtgttc tttccggagc aaaaaagcca tttccattat attggctcat tgacaactcc
    49561 gcctaccagc gggccaatcc aatggtttgt aatggatcat gtaggaaaac tgaaccaaga
    49621 gtttcttctc cccttcgatg gtcagtatgc tcgaccgaac aaccgaccaa tccagccctt
    49681 gaatggaaga gaaatttatt atttcgagga gtttgagcaa tgaatattca gcagatgaaa
    49741 tacgtggtcg ctgtagcaaa caatggtagt ttcagagaag cggcaaagaa aatgtttgtc
    49801 tcccagccaa gtctttcagc tggtatcaaa gaattagaaa ccgagttagg gatcacgctg
    49861 tttactcgaa cgaatcgagg ggcatattta acagaagaag ggcaagactt cttaaatcgt
    49921 gccgagaaaa tattggtcca gcttgataca ttagaaactc actatctaac gagtgaaaag
    49981 agagacaatt tttcgatcgc tgcccagcat tatgatttct taggtccttt gaccgcacag
    50041 ctactcgagg aattcaaaga cgagatcaaa caatttcgga tcatggagac gacaacagcg
    50101 agagtgatcg aagaagtcaa agagcagcac agtgagatcg gcattattta tatgaatgag
    50161 cataatcaag ctggtatcag aagatattta caacaaggag agctaagtgg ccgcagcatt
    50221 ggaaaattca aaacgcatat ttttttaagg caaggacatc cattagctga acacgcagag
    50281 atcaccaaag aagaattgtt atcttatcct caagttcgtt ttacacagga tggaaataat
    50341 ttcccgtatt tttatgaaga ccttatcgag atccctgatc aagaaacagt gatttatacg
    50401 agtgatcgag gtactttgat gaatatcgtt cttgaaactg atgcttacgc ttcaggatct
    50461 ggtattgtga ttggagggat tcgcgagcac ttgcggttaa tccctttggc agaaggcgaa
    50521 ctaaatgaat tttatatcat ttattcggcg aaacgtccgc tcagtgaaat cgctcaaaga
    50581 tttatcaaag agcttactcg gttactggac cagcagagct aagaaaagca gatgagaacg
    50641 ctgtttattg aacaccgttg atcaaaaaag aggggttgtg acagaatttt gtcacaactc
    50701 ctctttttgt agcaaacaat ttataactaa ttgctgttgt gggaaagtac gcaaatatga
    50761 ttctagaaac actcattgat tacctgtttt gtttcatagc tgtaattttt tcttcttctg
    50821 gtgtcacgat aatcgtcttt tggttttcat aaataacata accaggcttg gcgccatttg
    50881 gttttcggat atgctttacc tgtacaagat caaccggcac ttgggcagag tatcggtatt
    50941 ttgaaaaata ggcagctaac tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac
    51001 ttttaatgat gacgtgcgaa ccgggtatat tttttgcatg aagccaatag tcggtttttt
    51061 tagctgtctt cattgtcagc tggtcatttt gaagattgtt tttaccaaca agaatgagcg
    51121 ttccgtcaga agaaaagtat tgatcaggct gagatggttt cttacgtttt tgttttttta
    51181 agttcttctt tttcaggtat ccttctgaag tcaattcttc tttaattgct tcgatatcca
    51241 ttgggccggc aatttccagt tgagataaga cagattcaag atattggatt tcatctttgg
    51301 cttcttggat ttgttcgcca atcagcttaa cagcgttctt cagcttttgg tatcgatgga
    51361 aatatttctg tgcattttga tttggtgtca atgccggatc tagtgctatt ttgattggcc
    51421 gatcttcttc ataataatta ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca
    51481 gaaaagtagt aagtagttcg ccatttctac ggtaattctc agcgttttcg gattctttga
    51541 gtgtttgttc acgttttttc aatttatttt tatttcgttt tagttcattt tcgatttttc
    51601 gaatcagttc tccgccttgt tgttttgccc gatctttctc tgctttttcg tgataatagc
    51661 tatctaataa ttgactgagt gttggataag ctgttacagc agatgcctgt tcggacaata
    51721 cctgataagc aatcggtgtg aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag
    51781 gctgatggct gatggctgag aaaaattcgt cccatacaac catcttttca ttaggacgtt
    51841 ccagtaatct tttggtcagc tcttgagcga tatcgaaacc gattccctga aactgacgtt
    51901 ggattgcttt tggatctagt tcagtttggg aaagccgctg aaagattttt tctttctcgg
    51961 agctaaatgg gttcaagacc tcttgttttg gcggagcaac atattctaca ccaggaagca
    52021 aagaacgata agtgttttga gagcttccaa tgtgtttgat cgcatccagt atttttccag
    52081 tttctcgatt aactaagacg atcgtactat gacgtcccat taattcaacg atcaatatga
    52141 tattttgtag atcacccaac tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat
    52201 tttcgatttg ttcgatgtct tctaaaatag cgccatccaa atattttcga agcatcatca
    52261 cgaagttagg cggattatca ggattctgat aatcgatttg ggtaatctgt acacgagcat
    52321 aacttgggtg cgcagataat aataatcgat ggtttttgcc gcgtgaacga atcaccagca
    52381 cgatttcatt ttcataaggc tgatggattt tcgagatccg gccagatagt aacgtttcac
    52441 gcaattcgtt gaccatcgcg tgggtaaata caccatcaaa ggacatggtt cattcctcat
    52501 ttcttttcat aagtatattc attataacga aaaaaagaag aaaaaccaat ttggccgaat
    52561 agtaaagcaa aagtctagaa aaatcttgga ctaattacat aaaaaacagt gtttctatcg
    52621 ttgaaatcca aatttttagg tataataaaa aataggagaa aaagtagaaa agaaggtgtt
    52681 tgttatttgg gaagtagtta aatcgagagt acaagaaatg aatgaacatt tctcagtgtc
    52741 attcgcagaa aatccaagta agtattttcg acgtcttgtt tttctgctca tcttatcatt
    52801 gctgttaaca gttgcaatgt ttttattgcc ggaaaaaatc cgagaagagc aagtaaatag
    52861 tctcgtttat tcgtccatac caaccagcaa attgaaacct tttgattaca gcaaagctga
    52921 tcagaaaatc aaacaaagcc gggcgatatc ggtgatgttc agcaaaccga acgggaaaac
    52981 ctatcaggac atgcttgatg tttttaatga tcctgagctt atggaagaat tgaatcgacc
    53041 gatcttttat tatccgatcg tttatgatgt acatgaactg gaacaaaaat ataacatacg
    53101 aacggatgaa gtgacattta ttttctttga tggaggaaaa gaagcaaatc ggataactgc
    53161 gggtaaggga ggaataactg attttgataa ggagttgatc cctgaactga atcgacttcc
    53221 gttagccaat atcaaacaat tagaagaaga attggataga acgggagtaa cttcccaaaa
    53281 ttccgagatt gctaattgag acattggcgc gatgaaacgg tgggtctata tagcgctatt
    53341 tttaattatc agaacaagtg atgtttgatc ttcaaaataa caaataaaaa attaaaaaat
    53401 atagttgacg aaattcaaaa attcgatttg acttaaatca attcttaagg aaagggtgac
    53461 ctgtaatgaa cttatcaaaa cgtatttcag cagaaaacaa aaaaaattgg cgcagctggc
    53521 gtagataagt tgtattaatg gtgagacttt cccatagata caacttttta gagtgcattt
    53581 ctaaagcttg tatctatgct agacatgaac attgactgcc gcatagatta gaatctatgc
    53641 ggttttttgt gtaaagacaa tgaccgcttt ggccattgtc tttttttgta gactggatac
    53701 ttgcctgatt ataataaata aaaagattga ctttgcagga ggaaaaaaat gaaaaacaaa
    53761 cgattaataa cagttgtagt aattatttta gtttatttag caggtgcatt tatttatgga
    53821 aaagcctcag gcgaaaaaga aagtacttcg acgaaccaga caaaaaaaac aacagttgtt
    53881 ggggtattgc aatacgtgag ccatccggca ttagatgaga tttatcgagg aatcaaagat
    53941 ggtctgaaag aagaaggatt aaaagaagga gaaaatcttg agatcaaatt tcaaaatgga
    54001 caagccgatc agagcaagct agccacaatg agtcaacagc tagtacaatc tgatccagat
    54061 gccttgattg gaatcgcaac acctgccgct caatcattag caaacacgac ttcatcgatc
    54121 ccagttgtgt taggtgctgt aacggatcct gtgggggctg gattagtcgc ttctttagag
    54181 aaacctggtg gtaatgtaac aggtgtttct gatcagccgc ctgttacttc acaaatcaag
    54241 cttgcttctg atttgttgcc aaatgcaaaa aaagtcggaa tcttgtatgc ttcttcagag
    54301 gataattcaa aataccaagt agcacaagca gaagaagctg caaaaaaaga agggttagaa
    54361 tcagtaaaat atgcggtgcc ttccactaat gagattgcac aaacggttca agtgatgagc
    54421 cgacaagtcg attttatcta tgttccatta gataatacca ttgccaatgc gatgcaaaca
    54481 gtggtaaaag aagcaaacaa agcaaatatc cccgtcattc catctgtgga tacaatggtg
    54541 gaacaaggtg gactagcaac aatcgggatc aatcaatatc aattagggct tcaaagcggt
    54601 aagatggcgg cagaacttgc gagtggaaaa gaaaaaccgg aaacgacgcc tgtttataca
    54661 tttgatcaag gggatacagt gatcaatcaa tctcaagctg atcgcttagg aatcattgtt
    54721 ccccaatcga tgaaggaaaa agccaaaatc attacagatg actcgcagca agaaacatct
    54781 aaggaggatg acaaatgatc gtatcagcta tttcacaagg catgctttgg gcaatattag
    54841 gattagggat tttcatgact tatcggatct tagactttcc tgatatgacg acagaagggt
    54901 ccttcccatt aggtgctgct gtctgtgtga cagctattac tcatggggta gcgccgattg
    54961 tagctacatt attaggcgta ggtgctggca tgatagcagg actcgttacg gggttacttt
    55021 atacaaaagg aaagataccg gttattttag cagggatttt agtgatgtca ggattgaact
    55081 cagttatttt gtttgtgatg cgaacaccaa atctttcttt attgaatcat ccgattctac
    55141 aagacagctt ccatcgcttt gggttacctg attactttga cacagtcctt cttggcttca
    55201 tctctttggg catcttgatt gctgtactgc tattcttctt caatacagat ttgggtcaag
    55261 gctatattgc gacaggagac aacgaaacga tggctcggtc gttagggata aaaaccgatc
    55321 gtatgaaaat tttaggttta actttgtcga acggtgtcat tgcattatcc ggtgcgctga
    55381 ttgcccagaa cgacggatat gcagatgtaa ataaagggat tggcgtaatc gtcatcggtc
    55441 ttgcttccat catcatcggt gaagtgatct tccaagaatt gacgctagca gaaagattga
    55501 ttgctattgt tgtcggcagt atcatttatc agctattgat tttagtagta atcaaactag
    55561 ggtttgacac gacttatctg aagttgttct ccgctatcat cttagctatt tgtctgatga
    55621 tcccgcaatt gaagaaagca ctgaacttaa aaacgggttt tgagaaggag gcatagtaat
    55681 gactgatata ttgacaatca aaaatggcag aaaagtcgta aacaatggaa tgaatgaaga
    55741 gaaagtacta ttggatgaaa ttaaccttac aatcagagaa ggggacttca tcactgtcct
    55801 tggcgggaat ggtgctggaa aaagcacact gttcaatacg atcgctggta cgcttcaact
    55861 cagtcaagga tcgatctctt ttaaagatca gctgatcaca aaagattcag aagaaaaaag
    55921 agcacgattc ctttctcgag ttttccaaga ccccaagatg ggaacggctc ctcggatgac
    55981 agtagcagaa aatttgctac ttgcacaaaa aagaggtcaa aaacggaaat tccgtttacg
    56041 taacttgaaa aatcaaaaag aagatttttt cagactatgt caagaagtcg gtaatggcct
    56101 agagcaacac ttggatactc cagcgggtga attatctggt ggtcaacgcc aagcattaag
    56161 cttgctgatg gcgacgatca cagctcctga gttactatta ttagatgaac atactgcagc
    56221 tttagatcct aaaacagcta aagcattaat gcgattaaca aatcagcgga tcaatgaaca
    56281 aaaattgact tgtctcatga tcacgcatcg aatggaagac gccttgaaat acggaaatcg
    56341 tctgatcgta ctccaaaaag ggaaaatcgt tcaagatatt tctgaaaaag aaaagcaaca
    56401 tttgacaatg ggcgaccttt tgcagttctt tgaagaagta gtggattgat ttcgacagaa
    56461 aaattccttt cagtagaagg acttgaaaac tcattgacat cttcctttga ttcatcctat
    56521 acttaaaaca ataaacaatt aaacatattt gggtgttttg taggatcacg tagtcaatga
    56581 ttttaacaat taaatagaat attctttgct ctgacaagta aacgatgaga aaagaatttg
    56641 ctactttgcc acctagacgt ttcgtacttt ttcgtatcaa acgctaagtg gcttttttta
    56701 ttgaagtaag aacaaaggag gaataagatg atttctggac aaaccaaatt agctgggctc
    56761 tttgctgatc cggcttctca cagtttatcc ccattgatgc acaatactgc ttttgaagca
    56821 aacaacatcg atgcggtgta tttagctttc caagtaaatc caatgaattt gaagcaagca
    56881 gtcgaaagta ttcgaacttt tgatatgtta ggtgtaaatc tttcgatgcc gaataaaaca
    56941 gcggttattc cttatctgga tgaggtaagc caggaagcgc aattgatcgg agcagtgaat
    57001 acgatcgtgc acgaaaaggg tcgtttgatt ggctataata ctgatggtgc gggcttcatg
    57061 caatccgtgc aagaagctgg gatttcgatc aaaaagaaaa aaatgactct actgggagct
    57121 ggaggtgcag caaaggcagt cactgttcaa gcagcgttag acggcgctga ggaaatcgta
    57181 gtttataaac gaaacaacca aacattcgct caagtaaaag aaacattcga acaactcagc
    57241 tatcgaactt cttgtaagat aaaagttcgt gactttgccg atacgcatca attaagaaaa
    57301 gacttgaaag aaagctgtct tctagtcaat gcaacagata tcgggatggg aaagaaaatc
    57361 aatcagtcac cgctcttaga tataagcatg atgcatgaga atctagcggt ttttgatttg
    57421 atttatgcac ctagagaaac acgtttatta aaagaagcaa aaaaagtagg agcaaaagga
    57481 tacaatggac ttggcatgtt gatctaccaa ggagcaatcg cgtttgaatt atggacaaag
    57541 gaaaagatgc cgatccatgt gattgaaaag ctgtttgatt aaaaaaggat aatagataaa
    57601 gatcttttat tctcgcataa acggtctcaa gaaaagtaat cctttatgta tcgaagcatt
    57661 tatttcgtta ttttttatta aattatcaaa aaatgatagg cttttgtcga ttggttatcg
    57721 tatcctttat ttatcagtaa gaaacgagga ggaaccacaa tgagtcggat ggatcgctat
    57781 aaggacatcc ataagaaggc gaaacctctg aagcgggata cggggagtat ttttgctaga
    57841 agagaacgaa aacgaacgcg tatggaaaat gaggaagatg ctacccgtat ttatgaaaga
    57901 gagcagcatt catttgaaca tttgaaccaa tacgagcaac agatatacag agatgcacca
    57961 agagcagaag aacaacaaaa aaataaaaaa tttttcaatc ggcaaaagaa gaacaaaccg
    58021 ttaaaacctc gaagaaagcg atcgtggaaa aaaatcattc tatcgatttt gcttgttttg
    58081 atcctttatt caggtatctc attttttatt ggtaaatatg tagctgaaca tgacacaagc
    58141 ttagaggcag cgacttcaga aacctttcat gggcaaaaag cgacgaatgg tgcgcataat
    58201 attttgatcc ttggaagtga tacacgcgga gaagatgccg ggcgagccga tacgatcatg
    58261 gtcttacagt tggatggtcc ttcccataag ccgaaactga tttcttttat gcgtgacagc
    58321 ttcgttacga tcccaggggt aggacaaaat aagatcaatt cagcttatgc atacggagga
    58381 gccgatctag tgcgtcaaac tttagtagaa aattttggaa tcgattgtca atactatgcg
    58441 aaagtcgatt tcaaatcgtt tgaaaaagtg atcgatgcct tattcatgaa cggagtgaag
    58501 atcgatgcag aaaaagatct gaatctggat ggtgtagata ttaaaaaagg gggccagaag
    58561 atggatggac atgttctttt gcagtacgca cgtttccgaa tggatgaaca aggagacttt
    58621 ggccgtgtca gacgccaaca gcaagtaatg aacgctattt tcagccaatt gaagaatcca
    58681 ttgaatctga tccgtgcacc ttatgcagct ggtaaagctt taggatatac atccacagat
    58741 gtcactgctt ggttcttgac gaaaaacctc ttttcgattg cacgaggagc tggtggcatt
    58801 gatcgaataa gtgtgccagt agacggttcg tggaattatg ggcaaagtga ttatgctggc
    58861 agtattcttg aaattgataa tcaaatgaat caatctgcta ttacagattt tttgaataaa
    58921 tgatcataaa aaagatcaag aagaagctgc gcaaggcttt tgtcatgaat caatcaaacg
    58981 attcactcgt aaaaaacgct ttcttatggt agatttaacc aagagaggag ctgtttacat
    59041 tggatatcta tctgaacttc aaaaatgagt cgcgtgaagc catcgctttt tatgaaaaag
    59101 tttttcagac tgaagcatca ggtatcacca catacggtga aatgcctgga atagaacaaa
    59161 tgaacttgcc agaagaagca aaaaatctaa tcacgaatgc acggctgaac atttatgata
    59221 cattagttat gtttgcagat acgccagaag gaattggtcc tgaattagtt gtgggaaata
    59281 atgtttcttt agtcatccag acaagaaatg aagaagaaac gaagcgccta tttgaaagct
    59341 tagctgaggg tggtaagatt gagatgccac tggatgctgt tccgtgggct cagttgtatg
    59401 ggatggtaac tgatcgattt ggcatcaact ggcagattaa ccaagagtga cagctaaaga
    59461 tgaatatttt ctggaaacat caagcagcaa acggatcatc gtttgtttgc ttgatgtttt
    59521 tgtgtctatt tcgactatac ggtatctttt ttttattgcc agatgcgaaa atatgttcca
    59581 taatagagaa gtccgcaaaa aattaagtaa cggaggtcag aagatgaaga cggtgatact
    59641 agctgaaaaa ccttcacaag caaaagccta tgccgaaacc ttccaacagc aaaagcggca
    59701 tgaagggtat tatgaaatca gcgatccatt gtttgctggt gaagtaacca tcacttatgg
    59761 ttttggaaat ttggtagata tggttgctcc aggagcttat gaagagcgtt gggcaaaatg
    59821 gtctttagaa aatttgccta ttttcccaga aaccttccgt tacgaagttc caatggacaa
    59881 acgtgcacaa ttttcgatcg taaaacgaga attgcagtca gccgatacca tcattattgc
    59941 aacagatggt gatcgagaag gtgaagccat cgcgtggtcg attattcgac aagcaaacgc
    60001 attttctaag gaaaagaact atttacgttt atggatcaat tctcttgaaa aagaagcaat
    60061 ctatgaaggt tttaagaatc tccaacctgg aaagaactat tttccaaaat ataaagaagc
    60121 gcaagcaagg cagaacgctg attggctgat tggaatgaat ggaagtccat tatatagctt
    60181 gctacttcaa cgaaaaggga ttgatggcag tttttctctt ggtcgtgtac aaacaccgac
    60241 actctatatg atttatcagc tgcaagaaga aatcaaacac ttcaaaaaag aaacgtactt
    60301 tgaaggagaa ggtttaatta cgacaaacaa agggcagttt tctggaaaaa tcgtaccaaa
    60361 aaaagcattt aaaacccaag aagagttaac gaaggaaata gaaaaacttg gagcgcattt
    60421 aggtaagcaa aacggacgga ttcttgaagt tacgaaaaag gaaaagaggt tgcatagtcc
    60481 acgtttattt tcgctatcta gtttgcagac aaagatgaat cagctaatga aagcaagtgc
    60541 caaagctaca cttgatgctg ctcaaggatt gtacgaaagc aagtttctaa gctatccaag
    60601 aacggattcc ttttacatta cagaaaatga gcatcagtat ctggttaaac atctgcaaaa
    60661 ttataaaaaa tttttaggaa tagaagaagt acagacaact gagatcaaag caaagaaacg
    60721 atatgtcgat gcgaaaaaag ttcaagaaca tcatgcaatt attccgacaa agacgattcc
    60781 gactcctgca cgctttgcga agctttccaa attacagcaa gcaatctatt tgcaagtttt
    60841 gcgcactact gtggctatgt ttgctgcaga ttatatctac gaagagacga ccgtaatgac
    60901 gggcgtacag caattaaagc taaaaagcat cggaaagatc ccattacagc aaggttggca
    60961 ggaaatctta cgcatcaagt caaaaaagaa agaagctcag actcttcctc ctatcgtaga
    61021 aaatgaagaa gcagaagtag aactgaaaag tatggagaaa gaaacacaac ctccaaaacc
    61081 ctataccgaa ggaacgttga tcacagcaat gaaaactgcg ggaaaaacgc tggataatga
    61141 tgaagcgcaa gaaattctca aagaagtaga aggaataggg acagaagcta cacgagccaa
    61201 tatcattgaa aatcttaagc agaagcatta catcgaagtc aataaaaacg aaataactgt
    61261 tacgccaaaa ggaatcactc tttgtaaggc agtagccaat gaaccattac tgacgagcgc
    61321 agaaatgact gcgcgctggg aagggtactt gagaaaaatt ggtaaacaag aggggacacc
    61381 atctgttttt ttagagaata tcaaaaaatt catcctacat ttattggcag aagttcctaa
    61441 tcaaatcgac aaggtagact tttcagatga gattagcggg acacgtcaga taaaagcgat
    61501 ggagaagaaa aacgatcaat taggaagatg tccaaaatgt aaaaagggaa tagtcatgct
    61561 ttatcctaaa atcgctacat gcttgaatcc ggaatgcgac ttcaaattgt ggccaacagt
    61621 agctaaaaag aagctgacta aaacgaatct cagagatttg ctttcaaaag gaaagacaag
    61681 taaagttgtc aaaggtttta ctggtaaaaa agggaaattc gatgcagttt tagtattaaa
    61741 agaagatatg acaatcggtt tttcttttcc atggatcgaa aacagtggaa caaaagcagt
    61801 tgatggaaac aaagaaacat caagaaaaaa taatgaaaac gaagtagagt aaatgaaaac
    61861 aatgaaatag cggaatattt attccgaaaa gtgagaatat aaataccaat aaaactaaaa
    61921 aatatcagtg aatcaagcaa aaacgttggg gtaggctgat tttatataga tagcaaggag
    61981 tttttttatt tgaaaaaagt gcattcattt ttaaattatc ttatgggagc aatcacgatt
    62041 gtggctgtgt accaagcatt tctaaagtat cctgattacg ggacgaagtt gttgtcattg
    62101 acgcacaatc aaacagcata ttcaccaacg ttgattttat cgactttgac tgcaggagct
    62161 aaaagcgcta cttatttttt tcataaaacg acaagcatgc taaagcgaaa gaaacagcga
    62221 aaacaattgt tggtggtggt agcggctatg atcattattt attttctacc agttattaca
    62281 caagtatttc atcaatattt tggttaagaa tacgagagtt tgtgacaaaa gtcatgtcac
    62341 aaactttttt ctttgtcatt ttttcgtaag aaaaagcaaa ataaaagtat atacattgat
    62401 taaaagtata tacttggata taatgtactt actatagaat gagaatggta tagagggtgg
    62461 cgaaatatga aaaaaatgaa aaagtataaa attagaaaat ctggcaatag cgatgtaaca
    62521 actatcccac cagaagtaaa agaattcatg ggtgtgcaga caggtgacgc aatcagttat
    62581 gtttttcaat ctgatggttc tgtacgaatg atcaaagcac aagaagaacc tgatattgat
    62641 tcattagttg attcgatcat gaatcaatat gaagacgcgt taaaagattt ggttgattta
    62701 tgaggtatct ttcgactaaa gaaattatca aaatcaacgc gaaagttatt ttacgtcact
    62761 cgccaggcga aatgatcggt gtaaaagatg caaatgcatt agatatggcc gtcaaacagc
    62821 cttcacaagc agtattcaat caagaactct atcctgaaat ctatgaaaaa gcggcgattt
    62881 tagcaattaa tttagctaaa aaacatcctt ttcataatgg caataaacga actgctcttg
    62941 ttgccatgtt gttgtttctt caattgaata attgtccagt tgctttttct cgtcaagaag
    63001 ctgttgattt tatcataatg attaccacta gttcattgga tttcgattca ctaaagtcca
    63061 agataaccag ttatttacga caaaccgcaa taaaatgaac gaattaaaaa ctttatttct
    63121 caattagatc agcatgtcat taaggaatat gctgattttt ttatgtttaa tcatacaaaa
    63181 tagttttttc ataaaaatat ggaaaatatg gattaatttt gaaataattg atttaaaagg
    63241 aatacaaaag agtgaatagt aggaaatagt ggtttagaac tagtgggctg attttttata
    63301 tattttagtt tacataatat atattataca aagtagattt aaaaaatgat aaacctctct
    63361 cgaacggttt tttatcaaaa atcgtcattt ttccatcaaa atctgcttat gcaaaattat
    63421 aaaaaaattt tgcagttttt cttacttttt ttgtcgttta attgaatgat aaaaaccagt
    63481 ttctttattc agttaatcaa atatgaacga attagatatg aatcattgta tttgccgttg
    63541 cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca
    63601 acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac
    63661 cattctgttt atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca
    63721 gctgttaagc agttttagga tttttagttc atattcattg tttcttataa tacagtgttc
    63781 aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt
    63841 gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag
    63901 atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg
    63961 ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct
    64021 tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat
    64081 tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca
    64141 gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg
    64201 acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc
    64261 aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata
    64321 tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc
    64381 atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga
    64441 gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat
    64501 tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa
    64561 aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta
    64621 gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg
    64681 attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt
    64741 tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt
    64801 tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa
    64861 tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa
    64921 aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat
    64981 atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt
    65041 tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc
    65101 gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt
    65161 ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata
    65221 gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt
    65281 atcgatataa tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa
    65341 gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa
    65401 cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg
    65461 cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc
    65521 ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa
    65581 tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc
    65641 tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat
    65701 ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc
    65761 aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat
    65821 atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc
    65881 taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc
    65941 gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc
    66001 ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt
    66061 tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata
    66121 accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa
    66181 taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc
    66241 agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt
    66301 tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt
    66361 tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat
    66421 ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg
    66481 actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta
    66541 tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc
    66601 agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt
    66661 agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga
    66721 aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc
    66781 taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc
    66841 gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa
    66901 tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca
    66961 gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt
    67021 attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat
    67081 ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta
    67141 cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc
    67201 accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt
    67261 cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag
    67321 gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa
    67381 ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg
    67441 gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg
    67501 aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa
    67561 gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta
    67621 tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa
    67681 agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg
    67741 agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc
    67801 tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa
    67861 agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg
    67921 gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac
    67981 tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct actataagca
    68041 aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc
    68101 agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa
    68161 acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga
    68221 agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat
    68281 ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga
    68341 aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa
    68401 tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac
    68461 gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt
    68521 gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat
    68581 tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa
    68641 agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga
    68701 aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga
    68761 ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa
    68821 tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca
    68881 gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata
    68941 tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca
    69001 aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga
    69061 atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa
    69121 aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg
    69181 tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt
    69241 tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa
    69301 agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag
    69361 ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata
    69421 aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa
    69481 gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt
    69541 ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc
    69601 aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca
    69661 gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag gataaataat
    69721 ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg
    69781 ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca
    69841 ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag
    69901 aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca
    69961 tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg
    70021 atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt
    70081 ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca
    70141 aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc
    70201 gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa
    70261 cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg
    70321 gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga
    70381 gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa
    70441 tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt
    70501 gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg
    70561 atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc
    70621 actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa
    70681 gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg
    70741 acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga
    70801 ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa
    70861 ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat
    70921 cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt
    70981 ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc
    71041 catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc
    71101 tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc
    71161 actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata
    71221 tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct
    71281 ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga
    71341 acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca
    71401 aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat
    71461 cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac
    71521 tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc
    71581 agttttttgg ttgatccgat aattggatca tttcttctgt taataattca gatagcatag
    71641 gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga
    71701 gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt
    71761 cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca
    71821 gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat
    71881 cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt
    71941 gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt
    72001 cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt
    72061 ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa
    72121 aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt
    72181 ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag
    72241 gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca
    72301 tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga
    72361 cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga
    72421 tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa
    72481 ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat
    72541 cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag
    72601 ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta
    72661 gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc
    72721 caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta
    72781 atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat
    72841 cttccacttc taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa
    72901 cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca
    72961 aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca
    73021 aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca
    73081 catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct
    73141 ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt
    73201 tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg
    73261 tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa
    73321 tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc
    73381 gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct
    73441 tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct
    73501 tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg
    73561 ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact
    73621 tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa
    73681 tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt
    73741 atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg
    73801 aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat
    73861 gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc
    73921 accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa
    73981 tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat
    74041 tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta
    74101 aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat
    74161 atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg
    74221 acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt
    74281 atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg
    74341 tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc
    74401 gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca
    74461 ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt
    74521 ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata
    74581 ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca
    74641 aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga
    74701 tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg
    74761 atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc
    74821 cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt
    74881 ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa
    74941 agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat
    75001 ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga
    75061 cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga
    75121 ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa
    75181 aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat
    75241 tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca
    75301 ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat
    75361 tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa
    75421 tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg
    75481 tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag
    75541 ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt
    75601 atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa
    75661 atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat
    75721 atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata
    75781 cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca
    75841 aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc
    75901 tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca
    75961 ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata
    76021 tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag
    76081 atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg
    76141 tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg
    76201 gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt
    76261 cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt
    76321 gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc
    76381 taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga
    76441 aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc
    76501 acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc
    76561 agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg
    76621 ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg
    76681 gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc
    76741 ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac
    76801 aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag
    76861 aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa
    76921 gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa
    76981 tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg
    77041 caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat
    77101 ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag
    77161 taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat
    77221 agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa
    77281 ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata
    77341 aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt
    77401 ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga
    77461 ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc
    77521 ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc
    77581 caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct
    77641 cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat
    77701 acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga
    77761 atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag
    77821 aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg
    77881 ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg
    77941 ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt
    78001 ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt
    78061 caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat
    78121 gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt
    78181 attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg
    78241 cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat
    78301 ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg
    78361 cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc
    78421 cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta
    78481 aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag
    78541 tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat
    78601 aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat
    78661 cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt
    78721 tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga
    78781 agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt
    78841 tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct
    78901 tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag
    78961 aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc
    79021 ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga
    79081 aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc
    79141 gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc
    79201 gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc
    79261 taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac
    79321 agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc
    79381 cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc
    79441 agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg
    79501 tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa
    79561 aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt
    79621 tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct
    79681 aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg
    79741 atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac
    79801 catattctct gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc
    79861 tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt
    79921 gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc
    79981 gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc
    80041 tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc
    80101 tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt
    80161 gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg
    80221 tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca tatttagctg
    80281 ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct
    80341 actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga
    80401 ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga
    80461 tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt
    80521 agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa
    80581 ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct
    80641 ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt
    80701 accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc
    80761 atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa
    80821 tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca
    80881 ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc
    80941 tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc
    81001 tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga
    81061 ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt
    81121 accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct
    81181 tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct
    81241 ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt
    81301 aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc
    81361 aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa
    81421 tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta
    81481 ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg
    81541 tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata
    81601 tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact
    81661 tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga
    81721 ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg
    81781 tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca
    81841 ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag
    81901 atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga
    81961 cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt
    82021 ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg
    82081 tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct
    82141 gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga
    82201 tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc
    82261 gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca
    82321 ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa
    82381 aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa
    82441 aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat
    82501 agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa
    82561 ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg
    82621 ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg
    82681 ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc
    82741 cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg
    82801 atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt
    82861 gtttcctttg catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg
    82921 atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc atcccattga
    82981 tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa
    83041 ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta
    83101 gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt
    83161 tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta
    83221 tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg
    83281 tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta
    83341 gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct
    83401 ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc
    83461 ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga
    83521 tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct
    83581 attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc
    83641 agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg
    83701 aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata
    83761 attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt
    83821 tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa
    83881 aaacatatga tcaggttgtt ggaacgaaac tatagaaaga tggaaaaaat cattcgaatt
    83941 ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca
    84001 gaatctacat ttgtaaggta taatcgaaga gatcaaaatc cgctggggaa cggatgtaac
    84061 gattcaatct tcacaaaaat tggggtatcg tcttgaacat ttgaatgtac cagcttattt
    84121 aaatgttctg atggaaatgt cgcaaagttc tacaaacagt cgtttattaa atgagattat
    84181 tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa atttccatca gccggtcttc
    84241 ttttacacgt aagttgaaac aatgcaatca aattttagaa agctatttat taaaagtgat
    84301 cgttgaccaa ggttttcagt taactagcct taaaagtgag ctccagttgc gtatctttgt
    84361 tacctttttc tttttaagtt actacggaag acgcacgttg ccatttccaa tcgaaaaaaa
    84421 gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg aatttgtttc aggaagaaag
    84481 cccatatgag caatctttct ttattatgta ttttgttgtg catctcatga gagaatccca
    84541 aggaactcca tttgaatgga aagctggaga ctcttatgta cagttgataa atatcgataa
    84601 aaaagacttc ttaatcttga aaaatctctt aggaaaattg aagtacagca gctatataaa
    84661 agtagtgaac tattttgtca atacgatttt ttcagaattt tcggagaaag aaaaaaatga
    84721 aatcagtgag cagatacgaa aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa
    84781 agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa atttattttt tctcccgtat
    84841 tttcccatac gatacgactc atttgacgat ccgtgcaact tactttgcac agcagatgca
    84901 acaccgccag atacgtctat atgctggatt tcgtgcagaa cttcaatttt attccactct
    84961 actgaatcag aatctgattt attatttatc tagctatatc ttttggatgc ttatcggtaa
    85021 tcctgaaatt caccattata cttctgataa aaaaatactg attatcagcg acttaagtct
    85081 tcgacatagt caatatattg aagaatatat cagtgatatt cttgctgtgc ataaaattca
    85141 ctctgagaca actgctatta ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga
    85201 tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt cctcatatat tgattgatga
    85261 tagtgtgtca ttcgcaaatg aagaagaact catccgctta ttcgagttat gaataatcaa
    85321 aaaacagcta tgtataaacc accctgataa aaatttcagg acaaatacac atagctgttt
    85381 ctttttattg tattccttca gcaatccaac gagatttata gcgaaagtct tttaacaact
    85441 cttcttttgt ccaattattt gcaatgagat ccgtcaaagt gttcattacg agggtcatgg
    85501 aaaagtatag gaaactaact gcttcttccg acgattgaat ggagaaatga ttgattctta
    85561 ataattccag ccatttttcg ttcattggag aatcagccga tagttctcta cgtttggttg
    85621 tctgctgatc atttcgctgg gttaacaagt ataacctttg ccaatagata ctttcgtgat
    85681 ctaagtcgca gcaccactct aaatattttt cgatccccaa aaagaaatgt tgtttgtttt
    85741 cgctaatgaa ttttaagata tcttgatgga ctatgtgaga actgcttttg atcgtataca
    85801 aataagcatc ttccaaatct tgaaaatatt tataaaaagc accgcgcgac atccccatct
    85861 tgtcaacgat ttctgagact ttgacttggc tgatgtgctg tgagcaaaat atctccagta
    85921 ataggtcatc gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca
    85981 tccacatctc tcctttatct ctgattatac gtggatagcc cttcattgtc atgaaggatt
    86041 cattccttct ttttctcttc ttctgtcaag tacccatctt ccattcgata aacgcgatca
    86101 ctccattgaa tcatacggac atcatgagta accataatag tcgctttttg tttctcatga
    86161 gcttctttaa ctagtaattt gacgacttca tatgcatgat ctgtatccaa actagctgtt
    86221 ggttcgtctg ctaagatcaa gcttggctca ttatataaag cgcgagcaat tgccactctc
    86281 tgtctttctc caccagaaag gtctttagga taactatttt taagtgactg gatatctaaa
    86341 gattgcagca aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact
    86401 agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat tagagctttg gagaataaat
    86461 cctatttctt taaagcgtaa atctgctcgt tttttttctg gaagctcagt tagtgagtga
    86521 ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa
    86581 gtacttttac ctgaaccaga aggaccaatg atgctgacga attctccttc ctttacataa
    86641 aaatcaattg ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt tttggtaact
    86701 tttttcatcg ttaatatttt tttcattttt attaacctcc tattgctgta attgggtcta
    86761 ctttggttat cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc
    86821 cactatatag caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca
    86881 gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc taccacccca acaataaagg
    86941 actgtgctac taaagattta gcgatgaaga aatttttgat cccttgtgct ttcattactc
    87001 caaaaatagc agtcttctga agtgtcatca cgtacatgaa aataccgacc acggcagcaa
    87061 ccactaagaa caaaaagtat atcatcgcat ttaaggtcat attttgggca gaatagccag
    87121 gaatactttc aatgaatgtc ccaatagtta ataactgtag acccttcgat gctttttctt
    87181 tcgaaatttt agctgaatcc tttattacga tcccattgat cggctgttct tcttcagaag
    87241 caaacggctg ttgtccatac ttcaaagcag tccacgtatc tagggacgca tagatgacgg
    87301 gagaaactgt gtaataagtt tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac
    87361 tgccaatttt gatcgtatct ccaattttat agcctgaatc agccagattt tgagagatga
    87421 tgatttcatt ctttgtttta aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt
    87481 ctttcgttgt gccgaaaact gtaacatttt ctttgttttt tcctttgatt gctccgctgt
    87541 acaatccgat tggtgctttc tctcctcctt caatgtattt cagatctcct cttgttaatt
    87601 gtgaagctgc aaatacttga tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat
    87661 catcgattgc ttttttaaac tcttctgaaa gtccattcgc tagtcctgac aacatgaata
    87721 cgacatacgc aatcaagaac ataataccga cgatcaagcc atatcgtaat ttagaatagc
    87781 gaatttcttt gatagctaga aacatttgtt tttcctccct atgatagatt gatagtgaca
    87841 aaatgtcact tgttgaatag tgtaactcta aagtgacaat ttgtcacttg tgttgttttt
    87901 ttcttatcaa taaattgtct aagacatctt tcgataaaac cttaacatga gtcataaaaa
    87961 atttatttaa gccttattta tgctttccat gataaactaa agtgaccaaa tcaatgaggt
    88021 gggaaaatga ttaaactatt agtagtagaa gacgataaag tattatccaa taatataaaa
    88081 gaaatactcg ctgcaatcgg cgagataaca caagtttatg atggtagtga aggactgttc
    88141 gagttggaaa gtggagttta tgatcttgca atcttggatt tgatgatgcc ggaaatgaat
    88201 ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac agactccagt attgattttg
    88261 actgcaaaag atagtttgga agataagatt gaaggatttg aacgaggtgc agatgattat
    88321 ctaacaaaac ctttctatcg agaagaactg atcatgcgcg tgaaagcatt attgaaacgc
    88381 tcgctaggtc tctttgatga aaatgttctg aagtataagg atatcagttg tgatttaaaa
    88441 agcaatgaag tcttgtataa agaacaagtt ttgcccatac aaggaaaaga gtttgaattg
    88501 ctcgtttatt ttttacaaaa taagggaatc attttgacaa aagagcagat atttgaccgg
    88561 atctggggat ttgattctga tacgacgatt acagttattg aagtttatat gagcaactta
    88621 cgaaaacatc tgcgaagtac tggattggat aaagaaatca aaaccttacg taatgtcggt
    88681 tatatactgc aaagggtgtg atgcaatgaa tggagatatt acacgaaagc aacgtagtcg
    88741 cttttttgcg acaaatgtag cagcatttgc ttttatcttt tttcttttgg gattcattac
    88801 tttacagata ttaaaccaat ctgcatacag agaaacagat ctgtcattag agaaaataaa
    88861 ttttgattct cgaacagtac aattagagat acaaagatat cagcaaggcg atcccttttt
    88921 aaacccgact caaacggaac aaaacagaac aaacatggaa cagccattaa tggaaccatc
    88981 aaatgaccgt tttaacactc aagtgatctt gtggtctaaa aatggtgaga tattgaataa
    89041 agcagctatt ggtgggcgtt tcaaccaaat caaagaatta aaaatggata cgaaaaatct
    89101 gaataccatc caaaatattg aattaaatga agacagtggc tcatctgccc tctctttccg
    89161 ttcgattacg aaaaaagcgg agacgaatca agatggcgtt gcctacatcc aaattttaga
    89221 aaatacaaat caaatcaaga attcattagc gacttttcgc acgattatga ttttgtgtat
    89281 gattgttttt tggattctct ctattggtat cagttattac ttgtctagtc taagcatgcg
    89341 ccctattatt ctttcttgga gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt
    89401 gcgcacaccg ttaacgatta ttcaaaatag tttggaacat ctttttacca agcctaatca
    89461 tactattatt gaagaatccg aaagcatcgc tcaagcattg tcagagacga ggcgcctcac
    89521 tggattgact tcggatttgc tgacaatcgc tcggaatgat tctgatcagc agctgctttc
    89581 aaagcaaatg atcaatacac aagaatatat cgaacaactt gttaaaccat ttcaagagat
    89641 ggccatcata gatggaaaag agtttattct ccataacgat gcaacattag aagtaccaat
    89701 tgatgaaaaa aaaatccatc aagttttggt tattttgctg gataatgcat taaaatatac
    89761 gaatccagaa gataagatca ttttggattc atctgttaac cataaagaat ggctgattca
    89821 cgtaaagaat actggccctt ctatcagtga agaagatcaa gcaaggattt ttgatcgatt
    89881 ttatcgggaa gatcaatctc gggcgaaaga gactggcgga tacgggcttg gcttggcaat
    89941 tgcaaaacag gtaatcgaac agcatcatgg acagatttct gtatccaatt atcttccaca
    90001 aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa aaataatgct tatccctaaa
    90061 aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca
    90121 gaagtagctt cttcggaaat aagccgaaat ttctttaagc tgaccacttc tgtcacaacc
    90181 tctttagctt acgcttacga ttttttattg cggtcttcaa tgtgtacatc accgcttgtg
    90241 tcaatatcca attcttctcg gctaacatct tctgtcactt tttcaacatc ttcatgttct
    90301 tctttatgaa tcccaacttc ttcacgaata actgtatgtt tgcttacatc tacttgctct
    90361 tctttgattg ggatagtaat cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat
    90421 tgatctgtta ctggtttgcg ttcgatgacg atttcttcac gtttgaccgg tacttctacc
    90481 gtttcagtat cgttgaccac atgtttgtgg atgtcgactt ctccagttgt cacatcatgt
    90541 gtatttacat ctaatttttc ttcttttagt tgaattgtat catcatctag atttggtttg
    90601 cgatcgatgt cttcagtctt gcttgtaccc attgtatcag tctcgccagg tacgtccatc
    90661 ggatcagttc ctggtgtcat attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca
    90721 cctacgtcca ttggatctgt tccagtgcca gtttctaatc cagtccccat tgtgcttgct
    90781 gggctaacag tatcttgtgt atccattgta tcttctgttg tttctggctg acgatagcct
    90841 tttactgtga tgacaaattt tccatcagaa atatctcctc tatattgata taaaggatca
    90901 ttttctggtg tagccgtctc agaatcatag gtatcaaaag aaaaagtatc ttttattttt
    90961 tcccataaag aacggtcttc ttcaccatcc atccgatttt catctgtttc aacatctata
    91021 ccagacaatc cgtcaaatcc tagtctagat gctgcttcat cagtagtata aataacgata
    91081 tcatctcgtt catacccttc gttacgcaac cgttcaatta ctgataatgc ttcttccctc
    91141 gtactatagc ttccgatgac cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa
    91201 tagatttatg attggtatat tgttacttat aaatttattc tataggagac gattgttttc
    91261 tacaaatatt tagattggaa tttgattaaa acaatttaat tgaaatttga aaacatttat
    91321 gttaatgaaa gtcatattct aataaaatgt catttgtttc tatatgaatt tttatgaaaa
    91381 atttcatata atttccactc gttgtgatgg tttaatttat ccaatccatc aataagcaaa
    91441 caacttatat aaaaaaggat taattctcaa tataattgct cctaccatag aataaatctt
    91501 catgtaaagt aatgaagtac caactatttc taatacttct ttaagaatta tcccaacaat
    91561 atcgttagac aaaccattat tgaataaatg gagagccaat cgattctgaa aaaatgtttt
    91621 ctaatttttc ggaaataaaa gaaagagcct cctgccatta gaacgaacca aatccatttc
    91681 attgctcatg aagtaaccac atagctaaaa agaggataaa tcaattaaaa gatcaaaaga
    91741 atgactgcac taataaaata actaaaaatg tatcttctta cttcatatct tttagctagt
    91801 acttagttaa aaatttgctt cctaagatca agacaaactg tctatcttca tttgatgaaa
    91861 tgcggtaaaa tatagaaaat gaacactatt ggagggaatt tgtaatggac agcaaaacta
    91921 gaaaacaact gcaagaaaag ttaaagcaac gtacacactt gctcccttcc gtctttcaac
    91981 agtttttaga agaaaatcgt tcttcccttt cattaggcag tcgctatgaa tacgcaaaag
    92041 attttcatct gtttactgat tatttgagaa aagtattttc tgaagaagtg gatgatcagc
    92101 tgattatcca tttagaaaaa caagattatc agaatttctt agctaaaatc tatcgacata
    92161 ctcgttcgtt ccaaactaca gcgaaccaag aaacggagca attgtttgaa aatagctatt
    92221 acgggatttc aaggaagcaa tattcattaa atcatttttt acgatttttg tatcaaaaag
    92281 gactgattga agaagagatt tctcttttgg gtacaagttt accggaaaca accaaagcag
    92341 ctccgcgata tatcgatgca gaacttttca aagattttga gcatttgttt atcccgattg
    92401 aaggctttca aacaagcaga gaagctagct ttgaagaaaa gaatagaccg aggaaccttg
    92461 caattactgc cattcttctg tattgcggat taaagatcca tgaagtagta gaacttaaac
    92521 gtaaagatgt atctttgacc aagcagattc ttaatctgaa tagaaaagaa aacagactga
    92581 ataaagtacc aataccaaaa gaagcgatgc catttctgga gaactatttt aagttaacag
    92641 cacaaaatac ggacgaaaat tcttttgttt ttcgttcctc acatgatcag caaatttctc
    92701 ctcggactat tcgccaaatt ttgagcaaaa ttacagtata taaaaacgta tcgccagaac
    92761 tatgcagaaa aacatatcgt tattatgcag aactatattt agatgatgct gacgcggtaa
    92821 attatttatg tggtaatcga tacctctcat cccattcttt tcaagatatc tgggaaaaga
    92881 tggttgattt ttcttatcac gaattagcgg cattcgttcg ggtttcttcc atataaaaca
    92941 aatgtatgat ctttttttca caataaacta tgttattata taaaggatta tgttacgatt
    93001 atcttgaatt tgtatattac ttaacaaaag ataatggagt gtgaatgatg gagaccataa
    93061 cgtattcccc tttgaactta tttacgaatt ttcaagaagc tgcagctaac ttttccgaga
    93121 ccccgattat ctttgatgag ccactttcag ctttcccaga attaggactt gaaacaactt
    93181 acaaaaaatg ctgcgaaagt gtgttgaaac gggcttatca attggctcat cttggagtga
    93241 aatccggaga caaagttatt atttataaga gttccgcttt tgacacatac cttctagctg
    93301 tttctgtgtc ttaccttggt gcagtgcctg tcatgacttc ttatcatcta ccaacaacaa
    93361 caatggaagt atttatcgat cgtttagaag accctttcat tttatttgat gatgagacaa
    93421 aaaagcgagt gagagaaatt tcaaatggaa caaaatcaaa acagattcct
//
LOCUS       DAFELO010000005        62540 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFELO010000005
VERSION     DAFELO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.005"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(68..184)
                     /locus_tag="LP452_000387"
     CDS             complement(68..184)
                     /locus_tag="LP452_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL2013145.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(257..1213)
                     /locus_tag="LP452_000388"
     CDS             complement(257..1213)
                     /locus_tag="LP452_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2013146.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1384..1752
                     /locus_tag="LP452_000389"
     CDS             1384..1752
                     /locus_tag="LP452_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL2013147.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1923..2354
                     /gene="mraZ"
                     /locus_tag="LP452_000390"
     CDS             1923..2354
                     /gene="mraZ"
                     /locus_tag="LP452_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL2013148.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2375..3334
                     /gene="rsmH"
                     /locus_tag="LP452_000391"
     CDS             2375..3334
                     /gene="rsmH"
                     /locus_tag="LP452_000391"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL2013149.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3339..3740
                     /gene="ftsL"
                     /locus_tag="LP452_000392"
     CDS             3339..3740
                     /gene="ftsL"
                     /locus_tag="LP452_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL2013150.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3741..5933
                     /locus_tag="LP452_000393"
     CDS             3741..5933
                     /locus_tag="LP452_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL2013151.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGIAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5959..6921
                     /locus_tag="LP452_000394"
     CDS             5959..6921
                     /locus_tag="LP452_000394"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL2013152.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6935..8296
                     /locus_tag="LP452_000395"
     CDS             6935..8296
                     /locus_tag="LP452_000395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL2013153.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8322..9410
                     /gene="murG"
                     /locus_tag="LP452_000396"
     CDS             8322..9410
                     /gene="murG"
                     /locus_tag="LP452_000396"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL2013154.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9425..10645
                     /locus_tag="LP452_000397"
     CDS             9425..10645
                     /locus_tag="LP452_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL2013155.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10801..12126
                     /gene="ftsA"
                     /locus_tag="LP452_000398"
     CDS             10801..12126
                     /gene="ftsA"
                     /locus_tag="LP452_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL2013156.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12149..13390
                     /gene="ftsZ"
                     /locus_tag="LP452_000399"
     CDS             12149..13390
                     /gene="ftsZ"
                     /locus_tag="LP452_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL2013157.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13407..14084
                     /locus_tag="LP452_000400"
     CDS             13407..14084
                     /locus_tag="LP452_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL2013158.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14098..14697
                     /locus_tag="LP452_000401"
     CDS             14098..14697
                     /locus_tag="LP452_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL2013159.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14712..14999
                     /locus_tag="LP452_000402"
     CDS             14712..14999
                     /locus_tag="LP452_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL2013160.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            15048..15831
                     /locus_tag="LP452_000403"
                     /pseudo
     CDS             15048..15831
                     /locus_tag="LP452_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            15852..16559
                     /locus_tag="LP452_000404"
     CDS             15852..16559
                     /locus_tag="LP452_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL2013161.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16821..19619
                     /gene="ileS"
                     /locus_tag="LP452_000405"
     CDS             16821..19619
                     /gene="ileS"
                     /locus_tag="LP452_000405"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL2013162.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19668..21194)
                     /locus_tag="LP452_000406"
     CDS             complement(19668..21194)
                     /locus_tag="LP452_000406"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL2013163.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21209..21856)
                     /locus_tag="LP452_000407"
     CDS             complement(21209..21856)
                     /locus_tag="LP452_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL2013164.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(22040..22369)
                     /locus_tag="LP452_000408"
     CDS             complement(22040..22369)
                     /locus_tag="LP452_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL2013165.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22546..23274
                     /gene="sfsA"
                     /locus_tag="LP452_000409"
     CDS             22546..23274
                     /gene="sfsA"
                     /locus_tag="LP452_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL2013166.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23290..24303
                     /locus_tag="LP452_000410"
     CDS             23290..24303
                     /locus_tag="LP452_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2013167.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24303..25580
                     /locus_tag="LP452_000411"
     CDS             24303..25580
                     /locus_tag="LP452_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2013168.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25643..28345
                     /locus_tag="LP452_000412"
     CDS             25643..28345
                     /locus_tag="LP452_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2013169.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28497..28814
                     /locus_tag="LP452_000413"
     CDS             28497..28814
                     /locus_tag="LP452_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2013170.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28844..29164
                     /locus_tag="LP452_000414"
     CDS             28844..29164
                     /locus_tag="LP452_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2013171.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29272..30732
                     /locus_tag="LP452_000415"
     CDS             29272..30732
                     /locus_tag="LP452_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2013172.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30800..31021
                     /locus_tag="LP452_000416"
     CDS             30800..31021
                     /locus_tag="LP452_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013173.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            31052..31234
                     /locus_tag="LP452_000417"
     CDS             31052..31234
                     /locus_tag="LP452_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL2013174.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31234..31647
                     /locus_tag="LP452_000418"
     CDS             31234..31647
                     /locus_tag="LP452_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL2013175.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31770..32951
                     /locus_tag="LP452_000419"
     CDS             31770..32951
                     /locus_tag="LP452_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2013176.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(33022..33186)
                     /locus_tag="LP452_000420"
     CDS             complement(33022..33186)
                     /locus_tag="LP452_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013177.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            33340..33413
                     /locus_tag="LP452_000421"
     tRNA            33340..33413
                     /locus_tag="LP452_000421"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33374..33376,aa:Arg,seq:cct)
     gene            complement(33482..34621)
                     /locus_tag="LP452_000422"
     CDS             complement(33482..34621)
                     /locus_tag="LP452_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2013178.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(34920..35555)
                     /locus_tag="LP452_000423"
     CDS             complement(34920..35555)
                     /locus_tag="LP452_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013179.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(35668..36303)
                     /locus_tag="LP452_000424"
     CDS             complement(35668..36303)
                     /locus_tag="LP452_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL2013180.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(36337..36798)
                     /locus_tag="LP452_000425"
     CDS             complement(36337..36798)
                     /locus_tag="LP452_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL2013181.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(36928..37359)
                     /locus_tag="LP452_000426"
     CDS             complement(36928..37359)
                     /locus_tag="LP452_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL2013182.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37377..37697)
                     /locus_tag="LP452_000427"
     CDS             complement(37377..37697)
                     /locus_tag="LP452_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2013183.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            37996..38772
                     /locus_tag="LP452_000428"
     CDS             37996..38772
                     /locus_tag="LP452_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL2013184.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIVADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            38787..38990
                     /locus_tag="LP452_000429"
     CDS             38787..38990
                     /locus_tag="LP452_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013185.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            39006..39344
                     /locus_tag="LP452_000430"
     CDS             39006..39344
                     /locus_tag="LP452_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL2013186.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            39331..39510
                     /locus_tag="LP452_000431"
     CDS             39331..39510
                     /locus_tag="LP452_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013187.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(39553..40023)
                     /locus_tag="LP452_000432"
     CDS             complement(39553..40023)
                     /locus_tag="LP452_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013188.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            40110..40808
                     /locus_tag="LP452_000433"
     CDS             40110..40808
                     /locus_tag="LP452_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL2013189.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            40986..41327
                     /locus_tag="LP452_000434"
     CDS             40986..41327
                     /locus_tag="LP452_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013190.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            41320..41991
                     /locus_tag="LP452_000435"
     CDS             41320..41991
                     /locus_tag="LP452_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL2013191.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            41997..42683
                     /locus_tag="LP452_000436"
     CDS             41997..42683
                     /locus_tag="LP452_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013192.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            42686..43435
                     /locus_tag="LP452_000437"
     CDS             42686..43435
                     /locus_tag="LP452_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013193.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            43447..43716
                     /locus_tag="LP452_000438"
     CDS             43447..43716
                     /locus_tag="LP452_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013194.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            43722..43886
                     /locus_tag="LP452_000439"
     CDS             43722..43886
                     /locus_tag="LP452_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013195.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            43879..44178
                     /locus_tag="LP452_000440"
     CDS             43879..44178
                     /locus_tag="LP452_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013196.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            44178..44480
                     /locus_tag="LP452_000441"
     CDS             44178..44480
                     /locus_tag="LP452_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL2013197.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            44520..44885
                     /locus_tag="LP452_000442"
     CDS             44520..44885
                     /locus_tag="LP452_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013198.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            45375..45515
                     /locus_tag="LP452_000443"
     CDS             45375..45515
                     /locus_tag="LP452_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013199.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            45512..45700
                     /locus_tag="LP452_000444"
     CDS             45512..45700
                     /locus_tag="LP452_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013200.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            45708..45902
                     /locus_tag="LP452_000445"
     CDS             45708..45902
                     /locus_tag="LP452_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013201.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(45935..46147)
                     /locus_tag="LP452_000446"
     CDS             complement(45935..46147)
                     /locus_tag="LP452_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013202.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            46272..46475
                     /locus_tag="LP452_000447"
     CDS             46272..46475
                     /locus_tag="LP452_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013203.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            46472..46744
                     /locus_tag="LP452_000448"
     CDS             46472..46744
                     /locus_tag="LP452_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL2013204.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            46745..46930
                     /locus_tag="LP452_000449"
     CDS             46745..46930
                     /locus_tag="LP452_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013205.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            46917..47264
                     /locus_tag="LP452_000450"
     CDS             46917..47264
                     /locus_tag="LP452_000450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013206.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            47224..47700
                     /locus_tag="LP452_000451"
     CDS             47224..47700
                     /locus_tag="LP452_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL2013207.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(47846..48574)
                     /locus_tag="LP452_000452"
     CDS             complement(47846..48574)
                     /locus_tag="LP452_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL2013208.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            48622..48900
                     /locus_tag="LP452_000453"
     CDS             48622..48900
                     /locus_tag="LP452_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013209.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            48902..49288
                     /locus_tag="LP452_000454"
     CDS             48902..49288
                     /locus_tag="LP452_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013210.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            49285..49665
                     /locus_tag="LP452_000455"
     CDS             49285..49665
                     /locus_tag="LP452_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL2013211.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            49777..50229
                     /locus_tag="LP452_000456"
     CDS             49777..50229
                     /locus_tag="LP452_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL2013212.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            50226..51953
                     /locus_tag="LP452_000457"
     CDS             50226..51953
                     /locus_tag="LP452_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL2013213.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESSFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            52017..53204
                     /locus_tag="LP452_000458"
     CDS             52017..53204
                     /locus_tag="LP452_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2013214.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            53176..53745
                     /locus_tag="LP452_000459"
     CDS             53176..53745
                     /locus_tag="LP452_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL2013215.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            53758..55122
                     /locus_tag="LP452_000460"
     CDS             53758..55122
                     /locus_tag="LP452_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL2013216.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            55124..55405
                     /locus_tag="LP452_000461"
     CDS             55124..55405
                     /locus_tag="LP452_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL2013217.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            55383..55709
                     /locus_tag="LP452_000462"
     CDS             55383..55709
                     /locus_tag="LP452_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL2013218.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            55699..56037
                     /locus_tag="LP452_000463"
     CDS             55699..56037
                     /locus_tag="LP452_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013219.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            56027..56392
                     /locus_tag="LP452_000464"
     CDS             56027..56392
                     /locus_tag="LP452_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL2013220.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            56399..57025
                     /locus_tag="LP452_000465"
     CDS             56399..57025
                     /locus_tag="LP452_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2013221.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            57025..57489
                     /locus_tag="LP452_000466"
     CDS             57025..57489
                     /locus_tag="LP452_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013222.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            57684..59993
                     /locus_tag="LP452_000467"
     CDS             57684..59993
                     /locus_tag="LP452_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL2013223.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            60005..60709
                     /locus_tag="LP452_000468"
     CDS             60005..60709
                     /locus_tag="LP452_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2013224.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            60706..>62540
                     /locus_tag="LP452_000469"
     CDS             60706..>62540
                     /locus_tag="LP452_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2013225.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        21329 a        10266 c        12876 g        18069 t
ORIGIN      
        1 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
       61 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
      121 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
      181 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
      241 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
      301 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
      361 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
      421 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
      481 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
      541 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
      601 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
      661 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
      721 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
      781 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
      841 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
      901 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
      961 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
     1021 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
     1081 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
     1141 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
     1201 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
     1261 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
     1321 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
     1381 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
     1441 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
     1501 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
     1561 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
     1621 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
     1681 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
     1741 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
     1801 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
     1861 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg ggggggcttt
     1921 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
     1981 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
     2041 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
     2101 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
     2161 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
     2221 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
     2281 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
     2341 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
     2401 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
     2461 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
     2521 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
     2581 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
     2641 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
     2701 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
     2761 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
     2821 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
     2881 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
     2941 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
     3001 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
     3061 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
     3121 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
     3181 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
     3241 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
     3301 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
     3361 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
     3421 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
     3481 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
     3541 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
     3601 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
     3661 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
     3721 atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
     3781 aaaatctaaa tccgataaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
     3841 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
     3901 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
     3961 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
     4021 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
     4081 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
     4141 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
     4201 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
     4261 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
     4321 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
     4381 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
     4441 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
     4501 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
     4561 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
     4621 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
     4681 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
     4741 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
     4801 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
     4861 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
     4921 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
     4981 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
     5041 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
     5101 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
     5161 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
     5221 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
     5281 tcgaaagcga aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat
     5341 cggcaaaaac cggaatagcc caaatcgctt ctgatacagg gggatatcaa acaggagata
     5401 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
     5461 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
     5521 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
     5581 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
     5641 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
     5701 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
     5761 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
     5821 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
     5881 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
     5941 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
     6001 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
     6061 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
     6121 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
     6181 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
     6241 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
     6301 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
     6361 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
     6421 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
     6481 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
     6541 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
     6601 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
     6661 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
     6721 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
     6781 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
     6841 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
     6901 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
     6961 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
     7021 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
     7081 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
     7141 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
     7201 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
     7261 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
     7321 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
     7381 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
     7441 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
     7501 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
     7561 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
     7621 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
     7681 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
     7741 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
     7801 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
     7861 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
     7921 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
     7981 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
     8041 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
     8101 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
     8161 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
     8221 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
     8281 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
     8341 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
     8401 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
     8461 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
     8521 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
     8581 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
     8641 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
     8701 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
     8761 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
     8821 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
     8881 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
     8941 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
     9001 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
     9061 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
     9121 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
     9181 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
     9241 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
     9301 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
     9361 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
     9421 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
     9481 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
     9541 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
     9601 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
     9661 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
     9721 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
     9781 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
     9841 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
     9901 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
     9961 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
    10021 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
    10081 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
    10141 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
    10201 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
    10261 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
    10321 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
    10381 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
    10441 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
    10501 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
    10561 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
    10621 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
    10681 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
    10741 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
    10801 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
    10861 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
    10921 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
    10981 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
    11041 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
    11101 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
    11161 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
    11221 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
    11281 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
    11341 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
    11401 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
    11461 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
    11521 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
    11581 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
    11641 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
    11701 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
    11761 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
    11821 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    11881 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    11941 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    12001 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    12061 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    12121 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    12181 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    12241 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    12301 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    12361 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    12421 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    12481 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    12541 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    12601 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    12661 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    12721 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    12781 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    12841 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    12901 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    12961 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    13021 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    13081 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    13141 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    13201 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    13261 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    13321 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    13381 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    13441 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    13501 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    13561 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    13621 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    13681 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    13741 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    13801 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    13861 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    13921 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    13981 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    14041 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    14101 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    14161 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    14221 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    14281 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    14341 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    14401 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    14461 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    14521 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    14581 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    14641 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    14701 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    14761 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    14821 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    14881 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    14941 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    15001 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    15061 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaagg cgattggatt
    15121 gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc
    15181 tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga
    15241 tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag
    15301 gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt
    15361 catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat
    15421 atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt
    15481 gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca
    15541 gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga
    15601 ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc
    15661 gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa
    15721 ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg
    15781 aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag
    15841 gtggtttact catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa
    15901 tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg
    15961 aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac
    16021 ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata
    16081 cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac
    16141 aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg
    16201 atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg
    16261 cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga
    16321 tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat
    16381 tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg
    16441 acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg
    16501 catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga
    16561 acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca
    16621 ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag
    16681 cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg
    16741 tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa
    16801 ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat
    16861 tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa
    16921 atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg
    16981 atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta
    17041 aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat
    17101 gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag
    17161 aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata
    17221 agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta
    17281 cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag
    17341 gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag
    17401 cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg
    17461 tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac
    17521 catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg
    17581 tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag
    17641 aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata
    17701 tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta
    17761 ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata
    17821 tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta
    17881 cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg
    17941 atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc
    18001 acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta
    18061 tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat
    18121 ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc
    18181 agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca
    18241 caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt
    18301 ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg
    18361 gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg
    18421 aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat
    18481 cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg
    18541 tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga
    18601 tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag
    18661 atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg
    18721 atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat
    18781 tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt
    18841 ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag
    18901 ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg
    18961 atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg
    19021 attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat
    19081 tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag
    19141 aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat
    19201 tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag
    19261 aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa
    19321 aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg
    19381 tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg
    19441 tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc
    19501 gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg
    19561 caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa
    19621 agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat
    19681 tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca
    19741 agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt
    19801 tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct
    19861 tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa
    19921 gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt gattggttcc
    19981 gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc
    20041 tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca
    20101 gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt
    20161 tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg
    20221 atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct
    20281 tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg
    20341 aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt
    20401 tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca
    20461 acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc
    20521 ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga
    20581 ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc
    20641 atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg
    20701 atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc
    20761 aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt
    20821 ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt
    20881 ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca
    20941 tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat
    21001 gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg
    21061 gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac
    21121 gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt
    21181 ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg
    21241 ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag
    21301 ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat
    21361 ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg
    21421 actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc
    21481 catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg
    21541 aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat
    21601 gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca
    21661 gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca
    21721 caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt
    21781 gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt
    21841 ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt
    21901 aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt
    21961 tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata
    22021 tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt
    22081 cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta
    22141 cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca
    22201 aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc
    22261 atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt
    22321 aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca
    22381 ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat
    22441 tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc
    22501 tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa
    22561 gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa
    22621 gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata
    22681 ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat
    22741 ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg
    22801 ctggcaattg atggtatttt agacggcacg atccttttac caggattaac tggtccattt
    22861 acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg
    22921 gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt
    22981 ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat
    23041 gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat
    23101 ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag
    23161 caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc
    23221 aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa
    23281 ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc
    23341 caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa
    23401 aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag
    23461 acttttttca atattactca tatggacacc atttatattg cttctccgaa tagtcttcac
    23521 tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct
    23581 ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat
    23641 ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta
    23701 ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat
    23761 gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca
    23821 ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa
    23881 gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc
    23941 ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta
    24001 ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa
    24061 caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca
    24121 gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat
    24181 ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg
    24241 atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt
    24301 taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag
    24361 ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa
    24421 gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac
    24481 tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac
    24541 tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc
    24601 aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag
    24661 aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag
    24721 cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca
    24781 gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt
    24841 cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga
    24901 caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg
    24961 taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg
    25021 gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata
    25081 gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc
    25141 tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag
    25201 gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag
    25261 cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac
    25321 tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca aatataacac
    25381 ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg
    25441 cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag
    25501 aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata
    25561 aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat
    25621 tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac
    25681 agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag
    25741 caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg
    25801 ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga
    25861 ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag
    25921 catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt
    25981 tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga
    26041 ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca
    26101 aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc
    26161 agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga
    26221 gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat
    26281 ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc
    26341 gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat
    26401 caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt
    26461 tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct
    26521 tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact
    26581 agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg
    26641 aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga
    26701 acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc
    26761 tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga
    26821 aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc
    26881 aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc
    26941 caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga
    27001 aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca
    27061 aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt
    27121 acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga
    27181 agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat
    27241 caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca
    27301 cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt
    27361 agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt
    27421 acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat
    27481 gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc
    27541 tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag
    27601 aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca
    27661 gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt
    27721 tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa
    27781 taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga
    27841 tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca
    27901 aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa
    27961 gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt
    28021 aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat
    28081 ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt
    28141 agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc
    28201 tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact
    28261 agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt
    28321 tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct
    28381 gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt
    28441 attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg
    28501 caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa
    28561 aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct
    28621 cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag
    28681 tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg
    28741 tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa
    28801 atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa
    28861 tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga
    28921 agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc
    28981 agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca
    29041 aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc
    29101 tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga
    29161 ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt
    29221 gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga
    29281 ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa
    29341 catttagttg ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat ggcgggatca
    29401 gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat
    29461 gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc
    29521 aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg
    29581 gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta
    29641 gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat
    29701 gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat
    29761 ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct
    29821 tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt
    29881 gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca
    29941 gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt
    30001 cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc agaaccattc
    30061 ctaggattat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc
    30121 tggttctttg gtattcatgg accaaatgta ttggcaccag tgctagaggg aatctgggga
    30181 caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca
    30241 ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc
    30301 gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg
    30361 attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc
    30421 ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt
    30481 atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt
    30541 ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca
    30601 ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg
    30661 actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc
    30721 gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa
    30781 ttttctagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa
    30841 agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg
    30901 aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa
    30961 ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta
    31021 gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag
    31081 tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta
    31141 tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa
    31201 aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt
    31261 ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg
    31321 accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg
    31381 caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt
    31441 cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat
    31501 tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg
    31561 aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg
    31621 ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa
    31681 aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca
    31741 ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat
    31801 cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg
    31861 aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg
    31921 aggaggatcg gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac
    31981 aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag
    32041 gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct
    32101 tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat
    32161 ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg
    32221 gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac
    32281 agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat
    32341 tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg
    32401 gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg
    32461 atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa
    32521 catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat
    32581 ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt
    32641 tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc
    32701 attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc
    32761 tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc
    32821 agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga
    32881 caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg
    32941 actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca
    33001 tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt
    33061 gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt
    33121 ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc
    33181 attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa
    33241 ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca
    33301 agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat
    33361 agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtataaat
    33421 aacccctgta tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa
    33481 actagttatc atttttatat aaactatcga acacagtatt gattttttct ttatcctgat
    33541 cttcaagctc tcgaacaata tgaagataag tagacattgt agtttctaaa ctgctatgtc
    33601 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgtg
    33661 tatgccttag tccatgcaaa gtaaattgct tttcaatccc taattctttt aattttttgc
    33721 gcaaatattt gctgacggcg tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa
    33781 aattatgagg gtttttgatt tcaaaattct cgaataattc tttttgattg attttaaact
    33841 tttttaagag atcaatcgta tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt
    33901 ttgtttttcc ccattcattt aatttataat tccatgtttt attaatagtg atgatttgat
    33961 cttcgaaatc gatatcttcc catgttaatc ctagaagttc agcaaatcgc attccagtgg
    34021 caccagcaac taacacgagc ataggagaag agtactgcgc agaaaggttt tcttctacga
    34081 gtttcattaa agatttaaat tgatcataat ctaaatattt atcttctttt ggcttcaatg
    34141 aatcttttcc tttgattact gcttttctag ttggatcaaa cggaattaat ccttcttcta
    34201 cggcatcttt caacgacgct ctaatatggt tattaaactt aataacggta gattttacat
    34261 ggtctttagc atacttattt aaaaagcgtt gatatcccac tctatctaaa tcagaaatca
    34321 ataccgctgg catatatttc tttatgttca ttaacgtatc ttcgtatttt ctataagtga
    34381 taggagatac tgttccttct ttataaagtt gcatccaatc ctcaaagtaa tctgataata
    34441 atagattttt gcgatcccct ttaaggcctt tgttcaagtt atattctaat tcgttggcgg
    34501 catcttttgc ttctcctttt gtcttaaatc ctgattttct tatcttcgtg tattttccgt
    34561 catctttttt gtatgaaatt tcgtattgcc aactatttcc acgtttgact aatcttgcca
    34621 taattgaatc agctctcttt ctctgataca ataggaacta taaagaagcc cattgtttgg
    34681 gttttattat tttttgcaca tcctttttgc ttgccggcgc aaggatgtgc tatttgtcgt
    34741 tttaatggta taattggatg tgtattagat catccaatca gaggtgttta ttatgattaa
    34801 agtgattttt aaaagaataa ctgaacttcc agagtttaga ttatttgttg attattggta
    34861 tgtctggttt tttattgttg cattgctgat aggtctagat gtcatattag aaagaaaact
    34921 taagtaagag ttgagttaat aaattgaatt cctaaatgag atattttgta gcccatattt
    34981 tgtttgccga catcacctaa tactatggag ctaatcgttt tttctaacag accaaaatca
    35041 gataatctat ttaagctacc atctattagc tcttgtgaat agccagacat gctgatattt
    35101 ccagtgaaat tttcgtttcc acgactcatt aagcatgcta acaattgaat ctcaatcgat
    35161 gttaattgat tcagtacaag aagataatac tcctcgttat cccatgaagc gctgttaatt
    35221 cgaagtaaac aattcttata aagattttta tacatatcta ttttattttg tgcctttgat
    35281 ttttctacct cgtcatttat cccttcgaat attccaatga gttggtcctt gttctctaag
    35341 ttgaaaacat cttctggaat ttgatcgcga atgttttcta atgattcgtt taaatttttg
    35401 tagaatttct cgatgcgttt gaaacgcttc tcgtttttag acccaaaata aagtgactgc
    35461 acaacgccac caatataagg aattgctcca ataccgcctt caactattaa ttcaagatat
    35521 tcttttttgg ttagtttttc attgctatta tccatagtta cctcctattt tgtcatttcc
    35581 tttacaagca ttgtgactcc tgatggcact cttttgtata ggttttatta ttgtgcacaa
    35641 tctaactctt agtggggcgt ggttcattta ttttttactt actttactat taatcatatc
    35701 ccattgagca taggattttt ctatgttcga tcctcttgaa ctaatttctc ctgtaaaagt
    35761 tactttatcg ccctttgtaa aggtatcagc attgtttaag tccttcgcaa aaattacata
    35821 tgaaacttta ggtgttgcag aaatatcatt ccaactttta tcatcgtact ctttgtctgc
    35881 tataatggct attctagtag tcattgattc gataacagtt ccttcaaaag tatatgtttt
    35941 accactaata tatttagtaa aagctgtatt ttgctcagta gctggagtaa tagaataata
    36001 actatctaag aaagcacccc agtcatgatt atttgtctct aaaaattctt caaattgagt
    36061 taggctttta ttgctgctgt tagatgtagt agtttgagta tcagatgatg agctttcctg
    36121 tttgttattg tcatttgcct cgtttgatga ggaaagcgag aaacctacgg tagtaaaaat
    36181 aatcccgata ataaggagaa tcataggtgt tttcttattt gtttttttga taaatcttac
    36241 aattaacatg attaaaccta taataaataa aacaatacca gtaaaaccaa atataccgtc
    36301 cattaataga tcctctttct cttttgatat tgaatattat aaaccaagca attctttttt
    36361 cttaaaatcg aattcttctt gattaataat cccttcatct aacaactctt tatacttttt
    36421 aatttcatct gcacttgaaa tttgtgtttc tttggaatta ttatctaata tagattgttg
    36481 cttctctata tattttttta aattttccat ttcattccaa tatttttttg taaacataac
    36541 ggtgttttca tctttcgtgg ccgctaatat acctccgcga ctttcgtttc cgcctagcaa
    36601 ggtaaattgt atataaccat tactcatacc tggttttttt aattgtacgg ctgatatatt
    36661 tttaaagggg atagtttttt ctcctttaat accttgatta actaaattta taaatccttt
    36721 tctaataatt gaaatagaat catcagttac tttaatcaag gtttttcctg gagattttat
    36781 taaaatctct ttttccattt tttcttcctc atttctgtga tatgattttt tttgtagaag
    36841 ctcatatatg aggaaagagc ccgtgttgca gcacgggctt ttttaatgtt ttgctgaaat
    36901 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
    36961 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
    37021 atactccata aatttttcga gattaaactg agattcatca gtcagttcgt tctcaatata
    37081 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
    37141 agttgtagaa gtatcgtaca aaactgaaaa ttcaaaatga gaggcgctga aatgtgcaag
    37201 ttcatgagat aaatgaaagg cttctgcagt ttcaccgtat agattttcat tcaaaaaaat
    37261 gattctgggt tttggatagt agaaaccagg ctctttcatc tccatataaa ctactttcaa
    37321 attgtattcg ctcaacattt ctttcaattt caaatacata caaaccatca ctccaactat
    37381 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
    37441 tcttttccat caaaagaaaa aacagtatcg tcttctgata aatccacatg tttaggggtt
    37501 tctctttctt ctctacctag aaggtagtct acagagacat cgaaatagtc agcaatccga
    37561 cttaattcat ctgaattagg agtattattt ttccactttg ctagataacc gttagaatat
    37621 ccaaaattaa tttctaactg ccgtatagat atctttttcc tcttcgccaa gtcttttatt
    37681 atttcatagg tattcatcga taaaacaacc tttctaaatg tttacaaaaa aagtttagaa
    37741 aaatacgcag aaataatttg actaattctg agtaatgagc tatactatat cttgtaaaca
    37801 gatttaacaa ctaaaaagac aacaaaaaac actattgata tgtaaatgca gaccgccaag
    37861 aaagctttaa aatcaatgtt tttatgtctt atttaattat gctcttagta tagagtatta
    37921 ctcagatact gtcaattgaa tttagaaaaa agttgttaaa tttgtttacg aatataaaag
    37981 aaaggaatga gaaatatgaa cacaccgcaa atttttaatt tcgaacaaaa cgaagttcgg
    38041 acaattttag taaatgatga accatatttt gtaggcaaag acgttgcgag tgttttgggt
    38101 tattcaaaca ctaaagatgc tttgtcgcgg catgtagatt tggaagataa gatggggtcg
    38161 cgaatcacga cctcaggtca atcaagagag atgacaatca tcaacgaatc tggtttgtac
    38221 agtttaatct taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacaagt
    38281 gaagtgttgc cagcaattag aaaacatgga ggttatctaa ctccagaaaa agtagaagaa
    38341 gctttgctta atccagatac aatcattcaa ttagcaactc aactaaaaga agaaagaact
    38401 ggaagattaa tcgcagaaca aaagattgca gagtacgaac ctaaaatctc ctatttagat
    38461 agcatattat cttctacaga ttcagtaaca attagtcaga ttgtagcaga ttatgggatg
    38521 tctccacaac agatgaataa attacttcat aaactaggta ttcagaaaaa agtcggtaac
    38581 caatggttat tgtgcaaaaa acacatgaac caaggataca caaaatctca tacaactgag
    38641 atcccgaaag ccgatggtgg cactaaaatt gtaatgaata ccaaatggac acagaaaggg
    38701 cgtctattta tctacgaatt actaaaaaaa gaaggatatt accctcaaat ggatttagag
    38761 gaaattggtt agaaaggagt tttagtatga ctgacattgc agaaatcact caacgagata
    38821 gagaaaaaat caaagaatat gtcgaaagtt caaagttctt aacttacacc atgcttgctg
    38881 aaagatttgg aattagcaaa agctacttat ctttaatttt aaacggtaaa aagacttctg
    38941 cagaagcaaa cagaattata gattcgatta tcactatgta cgaattgtaa agggaggaac
    39001 agctaatgca atatctagaa gcaaaaatcc caattccaga aggctatgta attatctccc
    39061 aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg acgttgccag
    39121 aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt ttaaagaacc
    39181 caagatatag aaatattatc gatatcgaga aaaacaaaaa tggatttgtc tattatccag
    39241 ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt ttagaaacaa
    39301 atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc gctagtgttc
    39361 agcgcaccgc taatcatcta cgctttagga ctttggggaa gcaggcaagc gttgatagga
    39421 acgattgttt acatggtttg gatttttatg gggcttgatg aagctgaagc tgagtacaga
    39481 gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag aaaagagaaa ctagaatgta
    39541 tattactatt actcagttta attctttcac taatttctct attgggaagt ttgtattttt
    39601 gatatcaaca attttgtgga tttgggttct atttagagta aatatcattt ttgtttggtg
    39661 attaaggagt ttattaaaat taattttccc tacttcaaat gcaaggtaac aagatatagc
    39721 acttttagga ggaatattta gcggtacagc ttcagagtat attctattat catcgactga
    39781 tcctccttta gcaattaatt tcttaaatgg agtagctgac catctgcggt ttaagttagc
    39841 ggatgaaagt tctaaattaa ctaacatagc tggttctgtt gaatagttag aaatgattac
    39901 ttttgtataa atcatatctg cagcgaagta agaagcatta agttcaactt ctagttgagg
    39961 gcgcattcct tttattttta tccctgttaa aaacgtactc aatataaaac ttattattga
    40021 catccattga aaaattgtta agtgaaaaaa attcaaaata aacaccacca gtttttaact
    40081 aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag
    40141 aagctgtgac tacgagctta caagtggcag aaagcttcga gaaaaaacat caacatgttt
    40201 taagagatat tgatgcacta aaaaaagatg tgtccaattt tggacagatg tttgtagaag
    40261 gtaatgaacc agattcatat ggcagaaatc gacgagtttt cttcattagt agagatggat
    40321 ttttcttgct ggctatgggg tttacaggaa agaaagctat ctacttcaaa caaaaataca
    40381 ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa tactgttcct caaacaattg
    40441 aagatatgat gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc
    40501 ttaaagatac tatgcgaatt agcggacaac aagagtttga aattaagcaa aaaggaaata
    40561 tgaaagttat gggagttcta gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa
    40621 aagtattctc aaaattttgg tctgaattta aacgtacctt ttcaatccca agatatggcg
    40681 agttacctcg taagagattc gatgatgctg tttcatttat tgaaatgtgg ttgccagaaa
    40741 ctgcgatccg tatggaaatt gatcaactga acagacaaca gagacttttt ggtgatgaca
    40801 atgaatagag ctgaagcgct aagaataggg acggcaattg ctaattgctg gtggaaatac
    40861 tataaaccaa tcatcctaag ccaacaacat attgacaagc aaaaagcatg gcaacaaata
    40921 aaaaagtgac tccgccggca agcaaagagt cacaaagaaa acacatcata aggagatttt
    40981 agcatatgga aaaagaactt tccactctag atcaatattt gattgatcct gattggggca
    41041 agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg aaatcttttg acgaatgaag
    41101 aactagcttg ggatcaagat gatttaggca accatgtaac tatttgggat catgtttatc
    41161 ttatccatct atcgaagcat tcgaataaac ctgaatatat ttacgtcatc gaagatggct
    41221 tgattgatgc gctagaagag tacgacagag ataacttgat tgatatctct tattacggac
    41281 caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaat caaagggaca
    41341 acgaactttg aaaaactttt tagtcgtaag ttaaataaaa ttctcaagaa aaaaggaaat
    41401 tttgattatt tatcttgggc tcacgcgtgg gagattatga aaaagaatga tccacaggca
    41461 acggtaacta ttaatgagta caaacactac agggttgttt ctggaactca tcaagacttt
    41521 cttgttgagg aatataaacc ttttcttatg gatgaaactg ggacttatgt atctgtctca
    41581 gtaacggtta aaggacacac ggaaaccgaa ttatttcctg ttttagatta tcgaaaccaa
    41641 ccagttgtta aaccaaatgc aatgcaaatc aataactcat tgaagcgatg ctttgtgaaa
    41701 gcattggctc tacacggact gggattatat gtatttcaag gggaagatat tccaacacca
    41761 cctagaatcg atacaaagaa attaaacatg ctagagacga ttctagaagc tttcaatgag
    41821 cagatgggta aagatatgac caaaacctta atcgaatatg ttaatgagca gacagataaa
    41881 ttagggctct tagctgataa cgttgaaact attgaacagt taagctatga gcaatgtgcc
    41941 ttgatggagc gagcaatagc agctaagaga aaagaattag ataagaagtg atatgagtgt
    42001 ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa gttcaaagga aaagacataa
    42061 gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg
    42121 atggtgatcg agtcattgaa attcgtttta ttgatcctag acgtttcacc gtacagcaac
    42181 gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg ccaacggact
    42241 tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa ataagcctga
    42301 aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc atcttagatt
    42361 tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct gcgaatcaag
    42421 aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc aaaacaggag
    42481 ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatg
    42541 cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac aaaataggtg
    42601 tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag aaaacaatca
    42661 aaaagttaaa tattggaggg taaaaatgac agaacatcga agttattatg cgattatacc
    42721 agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat acggagagat
    42781 aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact ttgcagattt
    42841 atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta agtttggata
    42901 cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata ggtacctacg
    42961 aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa aagtcaaaga
    43021 taataataca tctattaata atacatttaa taatacaaaa gaatatataa gagagttacc
    43081 gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt ataaaaatgt
    43141 tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg attaccaaga
    43201 gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata aaaactattt
    43261 ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg caagcagtgg
    43321 atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc aagaagccta
    43381 tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat tctaagaggt
    43441 ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta taaaaagggc
    43501 gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg aattgaagga
    43561 attaagaatg atcgttcgag ttggagtaaa ctcagatact atttcgcact ctatatcgat
    43621 ggaacgcaat taagaaattt agcatatacc aaattactat tcattgattg tgttaaaggc
    43681 ttgcaaaaac atcttaatga acttgagcag gtgtaaacaa gatggaccta aagacattta
    43741 cagcacagat cgaattaatg catcaagaag ctttaagaca aagtgtgtcg tacgaagaca
    43801 agtggctcaa cacatttcat ggcggacgtg agagcgcact tgatcaagta ctcaaattac
    43861 tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact
    43921 tattcagaag attggcagga agataaaaaa atagttgcag aagtccaaaa gctcggtaaa
    43981 tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat ctggcatggt
    44041 gatcgaattc tagtaacggg taccgctaaa cagttatctg aaattactgg tctgagcaaa
    44101 aatattattt gggatagagc taggagctta tggattgatt caaaaggaag acagtttagg
    44161 tatgtggagg agaaataatg gacgaactaa tcgcgaaagt agagcagtgg gcaaaagata
    44221 agggattgga tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa gaaatcgggg
    44281 aagttgccgc ttctctagct agaaaagatg aacatggttt aagagacgga attggcgatg
    44341 tagtagtaac cttgatcatc ttggcaatgc aaaatgatat ggatttatac gagtgcttgg
    44401 attttgctta tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt gttttcgtta
    44461 agtccagtga cctgaaataa ttccattaat ggaagaaata actgaatagg aggatagaca
    44521 tgattgatgc aaaacaagtg caaaaacaga aagatggaat gttgatgttt gaagcatatg
    44581 ttctaccatt tcttaatcaa tttgaagttt tggaatgctc tgccagcgga gaagaattgg
    44641 agtatgttgt aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa tttctttgca
    44701 ctattaactg ttgggatatg atagctccag gatttttatg tccagctatg ggagaatttt
    44761 tggaatattg tcgtcttgaa gatgcaggca ctttggattt agcttatctg gtatacaact
    44821 acctaaacat caacacagat catttgtggt ttggcactgc cgaaagaaaa tgggtagtcc
    44881 gctaaccacc aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg ccgccattgc
    44941 tgtaaggttc tttacgcgac tcaaggtcac agaaatataa atgcaaatct cttttgaagt
    45001 gatccaaagc tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga gatttgcgtc
    45061 aagaacctga gagttatttc caaaaaagct tccaaagaac cgaatgagct aaggacatca
    45121 tgtgtaaaca cataatatag ctctcctttc acggctaatc ataacatgaa aaatattcga
    45181 tgtcagtagg aaaaaaggtg aattatgaaa agaatattaa attatcccgg cagtaacaga
    45241 ttctgcaatt aaactccgaa gctgatgtat cagtatcaac tgtagaatat tatgagcgtg
    45301 gagaaagtca agcagtcaca aatgtaaaaa tcgataacgt caacggtctt atctgtttag
    45361 atgaaggcga tagtatgaat gaaattgctg cagaccatga attatcagaa actgctttaa
    45421 ttgatcaaat cgaattagct agacaatact ttgttccata tgcagatacc tggtccaagc
    45481 ataagaatga catcattttt ccagaggaac aatgagagca actgatccag taattatcct
    45541 tgaggaagcc aaatttattt ggactcacga agagatagag caagcacgct tgctcttttc
    45601 tcaaggagtt aagccgagca aagtagctga aataatgggt caaaagattc ttgatgtcgg
    45661 attgcttttg ctccatctag cagaaaaaaa tttgatttga ggtgaaaatg atgattcaac
    45721 ttgcaggcat ccaaacaggg aaaatttatt tttctggaga aagcaaaagc gaagccaacc
    45781 aatggctatt gaaaacttac acgaacaata agaagctaag gaaaaaatat cctgatttgt
    45841 tgctaaagga tgatcagatt atgccggaac cgatgatttt gacccgtaag gagggaagtt
    45901 gaaactaaaa aaccagccgt gtgggctgga gttgctattt ctttttaata atgagttttt
    45961 ctaaatttaa ttcattctca cgagtttgcg tccaataatc agaataatat tttaattgtt
    46021 taagagtagg aatgtcgtct tcggtaaaat tagaaaataa ttctggagaa ttatcttttt
    46081 ttaattgttc taatcctgat tctttaaaac gttgttcaac gagttttact aaattgttga
    46141 tattcatgat aaatcacctt tttaaattta atttcgattt gattaatcga tacaagtatt
    46201 atatcaaata aaaaagctga tttagggggt cttacaaaaa cactaaaatt ttttcaaaaa
    46261 gaggaggaac ggtggtgaaa gtcagagaac aattcaagat agtgagcgac gaagataatg
    46321 tttacaaaat aaagatcgtt aatattaatg aatatcgaga agcagatcac aaatatttgg
    46381 ttgacttata catgaacgat aaatatttgg gagaaggatt ttgtggagat tcactttttg
    46441 atgatgagcg aattttgaag gaggaaacag aatgatccac gaactaaaaa tactaccaga
    46501 atattttgaa gcagtgacga gcggacgcaa acaattcgag atccgcaaga atgatcgtga
    46561 cttcaaagtt ggtgatcagt taatactacg tgaatataaa aaatacatta aacatgaatt
    46621 acctggacta acgagaccaa catttagtta cactggtgac tcatacaaag cagagatcac
    46681 ttatattact gattacatgc agaagtccgg atatgtagtg ttggggatta aaggggtgaa
    46741 ttaaatgaaa gtatacatct tagctatcac agagggcaca tggatgttcc cagtcggttc
    46801 cggaaaaatc tataaatcaa aaacagctgc ttataaagca tttgaaaaat acaaaaaaga
    46861 aaacggtggc ggcactaacg caaagattct agtggctgat aactggcatg aagaaggtga
    46921 acgtaattga tttacctgtt cattttcgtg gtactaacta tttttatgct agtaatctta
    46981 ttcaaaaaaa gatgagtaaa gacgaacgaa aaattctggc caacttttat ttagtattaa
    47041 aaatcgaaca aaatgtgttt tcggaaagga gaattgtatg ggaaaaacaa aatcaaagat
    47101 taagaagaaa aaacgacgtt tggaacaaaa ggcaatccag aatggaacgg ctaagaaaaa
    47161 ataaaaaagc tacccctttc gagatagcag cgcaatacta ttctaccata aggggtggcg
    47221 tttgtgagat ttcattggtt aaaagattac caagaactag atgagcagat tctttactta
    47281 aagtggaatc ttaataaaag taagcttgaa ttgaatcgat gggtctgtgg tgatttagca
    47341 aacgtccgca tcgaaaagaa ttcgagaact tcttctttag aagagaatat ccaaaagata
    47401 gaaaatgaat tggaattgtt gatcgagcaa aaagaagaaa tgttattgct gatagatagc
    47461 ttttctggta tcgataatca aattgttaag atgaaatatg ttgatcaaat gagcttagaa
    47521 gatattgctg aatctgttgg gtatagctca tcgtatgtca gacagcgaca tgcagaaatt
    47581 agaaagacat tgaacttttt agatgagtat gaacacagac aagctgaccg attgaaaaaa
    47641 gaaaacgaaa ttgattttta caatagcgag aagtacaaag aacaactctc tttattctaa
    47701 aattacactg ttcactaaat gttcggtcat tgtagctatg taaacattgt tagcagcatg
    47761 atattctatt agtgtcaaaa aaatatgaaa gagccaagat atcccaaccg ttttattaat
    47821 tggtatctgt ggctctttta ttcttttaat tagtagttgg cataaagtta tttccattta
    47881 taaattgatc agctaaatca gcatcataat cagccgcgat aaattgcgct gctgatttaa
    47941 taaatgcatc catatcatga acatctttat tagtatgtct acgaacatag tgcgtttcat
    48001 cattaccaat ccaagtagca gctttggcta atctttggag tttaggaaaa tcattcaaat
    48061 aattttcaat tgttttacct aataacattt tttttatttt atcttcatcc tctgaatttt
    48121 tagatattgc ataatcttta attagaaatt ctagagcttt tctataacca actccagcaa
    48181 tttgttccaa ttgctcagat ttagcttgtg tggcttgagt ataaatatca acaaatgtag
    48241 gagaaacttt ttcaatattt tctggtaaat ctattttaat cggcggacgg taataatctt
    48301 taattaaaat attttcagaa tatccttcat cttcattaaa cgtatttata aattgtaaac
    48361 tataaaattt gttgcactca tcatttatac actgtactaa tacgccaaag attctatcat
    48421 tatcgaacgc actatatgat gaaaccccaa aatacacata cggagtcatt atatttccgc
    48481 aatgcggaca actatttggc aaataaattg tctctgtatt ttgacgatca cgatacttaa
    48541 tattaacttc ttgaagcatt aaaacacctc gcatcttttt tataaatcat accacaaaat
    48601 atctgatagg agagtaaaca aatgaatgat tttcatgaag cggtacttag tattgaagta
    48661 gaatcaagtt tggctaaagt ttataagaaa gcaattgaag cagagaatag tccgtacaga
    48721 gaaaattgga atggtaatca tgcacatgtg caagtggaga atgatgatta cagaactgga
    48781 atgaatacgt tagttatttc tttattgtca cacactctac ccaatctgca ggaaacgatt
    48841 gaatggtatg agcgaatggg cgcaaaagta atccgtacaa attacaaagg agagaattaa
    48901 aatggcaatg atcaaaatta gaacatccat tagtggaaca gagtattggg attcagaaaa
    48961 gaaaaggaca gtagttgttc cgaaagatca agaaccaaat tttgaagtaa aagaagaaaa
    49021 aggaattttg agtgatggca aatcatttgt tgctgtcaat ggcgaactca tagctcatag
    49081 cagtgaaatt cttgatagcg atggaaacac tgctgctaat tttgatggag acgaagctac
    49141 taatgatcag tccactgaag atacggatga actggacaat atgactgcaa aagaattgcg
    49201 tgcatatgct aagaaacatg gtattgatat tcctggtgct atccgtgcaa aaggcgatat
    49261 cataaatctt attcgtgaag tagaatgaaa tactgtcagt ttgacggatg cacgaacaaa
    49321 atagcaaagg gtatctactg tactgaacac aagagatcaa gcagatcacg caagaagaag
    49381 cagcaagcaa agtctgttta tcatcatgag aacaaaccat tctatcgaac gcaagcatgg
    49441 aaagatatgc gtcaatttat ttatgaaaga gaaggtggtc attgtcagcg gtgtggtcag
    49501 ttcatatttg gaaagagagc acacgtccat cacattgtac caatcaaaga caacgaactg
    49561 cttaagcttg atccaaacaa tctcatgcta ttatgttcaa aatgtcatcc aattgttgaa
    49621 aacgaaacgg aagacaaaaa agtttttcct tcgtatttca attgaagccc ccctattcat
    49681 tttcaaaatt ttttcgcgtg gggagatagg gtagcgggga gtcacgcgca tcgttaggtc
    49741 aaatttttca aaaaacaaag gggggtgtat acaaaaatga cgactaaagc gcaacgtaaa
    49801 gcgattattg atgaaaaagt aagtgctgaa aaagctcgta tcttagaaat aatgaatttg
    49861 tctgatttgt acaccatcac tcttgatcca ttaatcgaat catacttgga tatttttgaa
    49921 atttaccaac acaaatattt attgtggaag gaaaaaggat ttccggagac gcaaaaattc
    49981 acgaataaat caggagctac taatcaatca aaacatccat tggcacaaca agtagaaact
    50041 tgggcagata agaagatgaa agctctagat ttattaggat taacaaacaa agcaaaaact
    50101 ggtagactaa ttgctggagg atcgaccgca agaaaagatg aagaaattac acgcccagaa
    50161 gtaaagccag tagatgaact agcagcgcac agaaataaat ggcgtaagaa ggcaggtgct
    50221 gaaaaatgat tgaacctggt gtaaattatg ctgatttatt tgcaaaagaa gtaagaaaga
    50281 aacctaagaa gtatcctaag accgttcgtt tagcaataga tcgctggtat cagtggaaaa
    50341 aaagaaaaga tatttggttt gatgtagatc gcgcgaatga aatgatggat tgggtagaat
    50401 catttattgt tcacacaaaa ggagaaatgg tgggaaaacc gtttctttta gaaccctggg
    50461 aaaaatttat ttattcttgg atttatggtt gggttaaaga aaatgaaaaa ggccaaattg
    50521 tacgcgttac tcgtgaggct tatgtccaga ttccaaaaaa gaatggtaaa acattgattg
    50581 ctgtcggttc attaggatat gcaatgtacg gagaaggggc gttatcagtt gattgctatg
    50641 cttgcgcatc tgattttgct caagcgcaat atgctgccaa accttttgca gttacaatct
    50701 taaacaatcc aatcctactt gaaggaacta aaatatttag ggggccaaag ggaactgttt
    50761 ctagtattac ttatgactat attcatgaag atatggctta ctcaaataaa tttattgttc
    50821 aaaccaagaa tattgataat atcgagggat ccaatccata ttttgtattg aacgatgaat
    50881 tgcataaaca ggagaaaatg gaacagtatg acaattttaa gtctgctcag atatctttac
    50941 cacagccatt gatgtttaat atctcaactg ctgggaaagg ttcgtcgtct gttggaatgc
    51001 gtgtatatcg cgaagcaaaa gaagtgttga aacgtgatga taatgattca aactttgttc
    51061 taatctatga gccaaataaa aattatgatt ggacggatag aaaagtctgg gaaatgtgca
    51121 atcctaactg gggaatatca gtcgatttat ctgctttaga atcgtcattt aaaacagcgc
    51181 aacggtcagc tcattccaaa gctgaatttc taacgaaaca cttggatgta tttgtgaacg
    51241 gcgcggataa tttctttgaa caggatcaag tagagccatg tttagttacc acacaagaac
    51301 ttggtgattt aagtggcgaa ccatgttata tcggtttaga tttatcacgt acacgagatt
    51361 tgacctgtgt atctttaaac ttcccaacat gggatgaaga tggtaaagcg gttcttaaag
    51421 taaagcaact ttattttatt ccgaatgaag atttagagtt tcgagaaaaa gaagataatg
    51481 ttccttacag tgatttggct gaacaaggtt tcgttgaatt ttgcgacgga aaaatgattg
    51541 atcaagatca gattttgcag tacatagagg actgcatgaa tttatatgat attcagcaag
    51601 tgaattatga tccagcgatg agtgacaaac ttgttgagaa attagaaaac ttaggattgg
    51661 aatgcgttga agttgctcaa tatcctaaag tattgaatgc tccttttgac gatgtcgaac
    51721 ggttgtttta tgagaaacga attcaatttg ataatccatt attcctttat tgcactttga
    51781 acgttgtagc aattactaac atcaatggac aaaaagcgcc aagtaaacgt caatcaaaga
    51841 aaaagattga tgggttcgtg gcgtttttgt gtggtcataa ggaaacgatg aatcaaatga
    51901 cagatattga ttcagatgag ttagatgatt acctaagttc catctataga taaatagaaa
    51961 ggcggtgaaa aaaattgaaa ttacgtgatc ggttatcgaa tgcagtctat tcttttatgg
    52021 aaaaacgtgg atatatcgag gatatttttg gacactacac acgttatggt caaagatatg
    52081 tgacggattc atctatcatg gaatcttctg atgtttatga attagttcaa gacatatcta
    52141 atcaggtggc attggccaca ccaattgtta ttggtcccga cggcaacgaa gtcaaagatc
    52201 atcacttact aaatattttg aagagtccca atgattattt gacaggattc gaatttacca
    52261 aattggaaac aaatacttta ttgatcaatg gtgagacatt cccattaacg gatagggatc
    52321 agcttcattt ggcgtacggt gtaacaacta aaatcaatga acgacttcaa gaagaatttg
    52381 aaatgaatgg tcaaaaaata cctggtcaaa tgattcgaca cattaagaac atcggaactg
    52441 attcattaaa aggtgctgga ataattgatc ttgcaagaaa cactctagaa ggcgttctga
    52501 gcgctgaaaa agttttgacg gacaaatata ctaaaggcgg tttacttgcg ttcatgctta
    52561 aactagacgc ccacatcaat ccaaataata gcgcccaaac aaaaattgtc aaagctatat
    52621 tggatcaact ggaaggaacg caaaatgaga gtgatcattc tgttaagatg attcctttgg
    52681 gaaaaggata ttccatcgag acattaaaaa gtcctgttga tgacgcggca attttgaatt
    52741 atttaggtgt ttacaaaaaa gacttaggaa aatttctagg aatcaatgtt gatacgtatc
    52801 aatcgctgat gaagacagat attgaaaaag cgatgatgta tctgcacaac aaagcaatca
    52861 aaccaatatt gaaaaacaag agcgaacatt acaccgctct tttttttatg cctaattctg
    52921 gctatagagt ggaatggaaa attaatattt tggattttgt tccttactca acaaaaacaa
    52981 atattggcta caacatcgtt cgtaccggga ttacaagtcc agataacgtg gcagaaatgc
    53041 ttggttttcc taaacagaat actccagaaa cacaagctat ctatatttca aatgacttat
    53101 ctaggattgg ccagaaaaat gcaacagatg attccttacc aacgaatgat caagacttga
    53161 aaggaggtga tggaaatgaa gaagaaggaa attcgcacga ttgacatcac caatctttca
    53221 acacgttccg atgaagaaac tcatacgagg accattagcg gctacgctgc tgtattcaac
    53281 agtcaaacac ttttatggga tgacttaagc gaagtgattg ttcctggtgc attctcaaag
    53341 gcaattagta attcggatgt tcgttgtttg ttcaatcacg attggtccaa tgtgctagga
    53401 cgtactaaaa gcggaaccct tcgccttgaa gaagatgaac gtggtttgaa attcgaagtt
    53461 gatttaccgg acacaacggt agcaagggac ttagttaaat ctatggaacg aggagacatc
    53521 aaccaatgta gttttggttt tgtgccgact gaagaaacgt gggactacaa ttctgaacca
    53581 atgcttcgaa caatccatga agtggaatta tacgaggttt ctattgtgcc tttgccagca
    53641 tacgaagata cagaagctgc attaagaagt cgtgatgaat tagaaaaaaa cgtcgaagaa
    53701 agaaaaaaat taatcaaaaa aattaatcaa gcgctagaag cgtaggagga aaaatacatg
    53761 gataaagaat tattgaaaaa aatgaaggca cgtcgtgagc aacgattgac tgaattacgt
    53821 gaaaaagttg aatcaggaga attacgcgaa gcagatttag aagccgtgaa agaagaaatt
    53881 gacagtgtta ttgatgaatt gaacggaatt aaagacgaat taggcgcaga ttctggaact
    53941 gatgaaacag acgacaatac agatgatcaa tcgaatagta cggattcaga cgaaagccgt
    54001 tctggtgaag acaacgatca agaagaagat tcagacagtg aagatagttc agaaaatcgt
    54061 tctggaatga ttactcaaca gcaacgagat ggattacttg gatcaattaa gaacggattg
    54121 gaggcacgtg caaaaatgac caagaaacaa aaagatcaac aactacgaaa agcatttgct
    54181 aattttgtag ctggaaatat ttctgaagca gaagctcgag ctttagggat tgaagctggc
    54241 aacggttcag ttactgtccc agaagtaatt gcatctgaag ttattactta tgctcaagaa
    54301 gaaaatttac ttcgtaaata cggaacagtg gtgcgaacat caggagatgt caaatatcca
    54361 attcttgtga agaaagcaga tgcgaatgta aacaagaaag agcgttcaac tgatattgct
    54421 gaaacagcta ttcagtttga tgaaattttg cttaatcctg ccgaattcga tgctttggca
    54481 acagtaacta aaaaattatt aaaaatgtct ggtgttccag ttgaagatat tgttgtggaa
    54541 gaattgaaaa aagcttatgt gcgtaaagaa atcaattata tgttcaatgg tgatgacgct
    54601 ggaaatgaaa atcctggtgc attagccaaa aaggctgtag catttaaaaa acctttagat
    54661 ctaactgctt caggtgctgg gcaaaaatta tatgatgcat taatcgaatt taaaaataca
    54721 ccagtgacag aagtgatgaa aaagggacgc tttattatta atcgagctgc tttgactgcc
    54781 attgaaaaaa tgaaaacaga tgatggattt cctttgttgc ggccattcac acaagcagaa
    54841 ggtggaattg gttaccaatt agttggctat cctgtggatt ggacagatgc agcagataaa
    54901 aagggtgaac cagacacgcc agttttatat tttggcgatt tttctgcatt taaaattcaa
    54961 gaagttattg gtgccttgga aattcaaaaa cttgttgaaa aattctctgg taaaaatcaa
    55021 attggattcc aaatttacaa cttgctagat ggtcaattgg tttattctcc atttgaaccg
    55081 gcagtatatc gctacgaaat tacaaaacca gttggtggtt aagatggaag agcaaactaa
    55141 agaattgtct ttagaggaaa aattcaaatc acatattcat tttgaagagg gcatggatga
    55201 ttctttgctc tctttttatt taaatatggc aaaagattat gtcaaaacag caactggtgg
    55261 ccaacaagaa tatcttattt tgatggttgc cggcattgcc tatgaatata gagtttcaga
    55321 ggatgaactc gacaaagcta tgaatgccat gacgccattt atcgtgcaag gagcgattca
    55381 aaatgccgaa gagacagacc aataatctca gatggaaagc cgaattgcta gacatcaaaa
    55441 caggaacaga cggaaacgat cgtccaacta caatttacga atttaagcgt ctaatattct
    55501 atgaagaact cggtgtgact tctcaagaaa aatatttatc acagcaagcc aagacagacg
    55561 ttgtcagacg aattaaagtc agatgggata aatccatcac agagaaatta agtgcgctca
    55621 aaattgattc tgtaacgtat aacattactc gcatttatac gaatcccgat gcaagagaaa
    55681 tggagttgag tttagcttat gtcgattagc tttgatgaat tgaaaacagc gctgaaatca
    55741 acaaagttag cagtgttcag agacaaagcc agattaggga cgatgtatcc atacattgtg
    55801 tactcaaatg tgagtaacag caaaaaaatg gcatccggta aagtctataa aaaattacct
    55861 tattaccaag tctctttttt tacacttgga acagaacagg atttagttgt tattgaacaa
    55921 gcgctgcaaa atgctggcat tccatattca gattttacag gtatacaagg tgatgagaac
    55981 gacgataccg taaccaacta ttacacatat gtgaggtgtg tggaaaatgc caagtaataa
    56041 gaatggtttt tctgaaatat cggattattt aggaaacctt tcaaaagttg atccgaaaaa
    56101 attatcgttg gaatctttag aagaagcagc taacttttat ctaaagcagt tgcttcctaa
    56161 tattcctaag tcgctcttaa aaaagaaaca tatgcgcgat caaataaaag tagtagttga
    56221 aaacgatcaa gtgaaagttc aatttgaaga gacggccttt tactggcgct ttgccgaaaa
    56281 tggcacaaca aatcaacgtg cccaacattt tgcaagtggt acgtatgaac aaaacaaaga
    56341 aaaaattgaa gaaattatga ctagaaaaat acttgattta tgggaagggt gattttaatt
    56401 gggaaaacaa gatacttttt attttgaagg attggacgac attttaattg cgatgatggc
    56461 aacgccagat tctgttggaa ctgcaccgac ttatagtgaa gttttacgat tgccaattgc
    56521 cacaaaaatc ggtgtcaaag ggaatggaac agcattagaa aaatgggcat ccagcaaaat
    56581 gtttcgtcgt gtgtcgcgag aaacaaaaca tgaacttgcg ttggatcacg tgggtattcc
    56641 gatagaagtg atggatgaaa tcaaaggatt agttgctaaa agcggagtaa cgtttagcaa
    56701 aaataccgca cgagaatttc cgtacttcgc ctttggattt attggaaata ttgaaaatgg
    56761 cggtaaaaaa gcagtttggt atcctaatac acaactatcc aatgtgattg acgaagagta
    56821 tgccactgca gaggaagaaa caaaaattga cgatgtaact gcaaatctgg tttccatcgg
    56881 actgaaatac aataatgtga tgtatgcaag ttttgattca aatagagacg gagcttctct
    56941 agaattattt aataaattca ttgctcaacc tgtatacgat gaagaacagt ggaaaacatt
    57001 ggctaaagtt ggaggtgcgg gttaatggct cggttatctg attatggaat tcacgttgaa
    57061 gacttaaaaa attctgctgt tgtaattatt caaggcgcag aattccctat ttcatttact
    57121 atgcaaacga tggaatttat agcagatgtg tatggtggag attattcgca atttgaatct
    57181 gatatgaacg ccatgctatc taaaaaagaa ggacaaattt cttctgctaa cttatcgcct
    57241 agtgatctaa aaatcatgcg tgccttgatt tatgccatgc tgcgtactgg tggtttagag
    57301 gaagatccag aaactatttt caaatttttg ggaatgagtg gagaggtgtt gtctgcttat
    57361 agtacctgta tggaaatttt tgctagccag acatttcagg tggaagacct aaaaaaatcc
    57421 aagaagccac aagactttca aaaagcgcaa gcaaaaagaa agaaaaacaa aaagaatcgg
    57481 aagagatagg aactccttgg agtttttata tttacgttgc tctcactcta ttgaattgga
    57541 gtgagagttt ctttttaaag tctacaccta acttgtggct caaatcttat ttacagtggt
    57601 tacaacaaaa caccgatttt gaaccacctc aatctgtaac tatggataaa tcgccttggt
    57661 ggtagaaagg agcgctaaga taaatggctg gtaaagaatc tgatgtcgtt cttaatttta
    57721 aaacgaatgg cgaagtcagt tattcgaaaa caatcaaaga aatcaacaaa gaaatgaact
    57781 tagccgctgc cgagtacaaa aaccaagtgt ctgcgatgga caaggatgca actcaaacag
    57841 aaaaattgcg agcggctaaa caaaaattag aaaaacaatt aggattggct gaacaacgat
    57901 ctcaaatgct gagagaggaa tatgaaaaat ctgtcaaaga aaccggtgaa tattctgctg
    57961 aatcagaaaa actttataaa caattactca attctgaaac cggagaaaat aaacttcgta
    58021 cagcattaga acagacgaac gatgcactta aagaacaagg tgacgtttct gttgatacag
    58081 caaaaaaact ccagaaaatc gaagaaacag gtgagaaagt aaaaggcgtt ggtgaaaaaa
    58141 tgtctgttgg agtaaccgcg cctattgtag cggcaggagc agcaggactt gcagcatttg
    58201 gtgaagttga cgaggcactt gataccatca ttacaaaaac cggagcaaca ggtgatcaag
    58261 ctgatagact ttcacagtct ttcaaaaacg ttggttcaaa tactcattta cctttacaaa
    58321 cggttgggga agctattggt gaggtaaata cacaatttgg attcatggat aaaaaactgg
    58381 aagattcaac caattatctc ctacagtacg ctgaaatcaa tgatacagat gtttcgcaat
    58441 cagcaatatc tgctcgacaa gctattgatg cttatggact agaatatgat gatttgaatt
    58501 ctgtccttga tgtaacaacg aaaacatcgc agaatactgg tcaatctgta gacgacttga
    58561 tgcaaaaagc aattgatggc gcaccacaaa ttaaacaatt agggttgagc tttggtgaag
    58621 gggtcacttt acttggacaa tttgagcaat ctggtgttga ttcaagcgca gctttaagta
    58681 gtttatctaa agcaacagtg gcttatgcga aagacggaaa atctttaagt caaggactag
    58741 aagaactaca agacaaggta aaaaatgcag gttctgaaac agaagccatc aatgcagcag
    58801 cagaagtatt cgggactaaa ggcggtccta gaatggccga tgcaattcgt agaggtactt
    58861 tgaacctgga agatttagca aaaactgctg gagaaagtgg gggagctgta ggagacacat
    58921 tcgatgctac tctcgaccca attgatcaag ccgatcaagc tatgaacaat gcaaaattag
    58981 caatggctga cgtaggtgaa gcagtccaag taagcctttt gccttttttt gaaaaagcaa
    59041 cttccttatt acaggaattc tctaaatggt gggggtctct agatcaagat acaaagaatt
    59101 ggatcataac aattgctggt atagcggcag cagttggacc agtactaatc gttcttggtt
    59161 cacttatggg atctgttaca aaaatcgttg gggaagttaa aagttttata ggagtttggc
    59221 aaggacttgc tggtttattt ggaatgtcgg gaggctggtt tgcattagca gttatagcta
    59281 ttggcgcatt agttactgga ctaatttggg catataataa cgttaaatgg tttcatgatg
    59341 gagtgaattc tttcttcaaa ggtgtttcag atgtagctgt tgaaatattt aattttttaa
    59401 gtggttattt tggtggattt ttcgatggtg ttatagcaaa tttcaataat ttttataatg
    59461 ctggaaaacg aattttcagt ggttttctag attttatcac tggagtattt acagggaatt
    59521 ggtcaaaagc atggcaaggt ctagttgata tttttggagg tattttcgat ggtattgttg
    59581 ctgtagggaa agctcctatt aatgccatga ttggattgat caatggattt attggtggat
    59641 tgaataatat aaagatacca aaatgggttc ctggtgtagg tggaaaatca ttttctattt
    59701 caaaactgcc ttatttagca caaggcggtc atctaatcaa tggacaagcg attgttggtg
    59761 aagctggacc tgagttactg actgccaaga atggtaaaac cacagttact ccattatcag
    59821 acgaagaaaa acgcaaaggg atcagtggaa gagtttctgg aggtagcatt gaacagcata
    59881 tacacattgg caaagtcgat gcaaacaatc catctgagtt agatcgtatg aatcgtaaat
    59941 ttgctaaagc aaacaaacag gctattcgtg atttgggagg tgttcctatt tgagtcgaca
    60001 atttatgaat ccagatgaac caaattttat ttggaaaaat cggaatgcag taattgatat
    60061 gaattgcatc attgaggcag agcttccaga tattatgccg aacaagcgat acgaaactta
    60121 cacgattcag ggccgaagcg gagaattgac ggagacgttt gatgactatg aaccttttga
    60181 tttggaaata gaagggatca ctattccaca ttcgaaactg agagaagtca aacgatggct
    60241 cactggtaaa agccaattaa tcacacataa tgatccagac aagtatctag aggctatctg
    60301 cagcatggat aaagaagtcc ctttcgaaaa cgagtggggc ttcttttata cattcgatgt
    60361 tacttttcgt tgtcagccac tcaaaagaaa aatcggtgaa gcacctaaaa tttttcattc
    60421 tagtgttatg gatatttttg atcatggtga cgaaattgca catccctatc tggaaataga
    60481 atcgaacgga ggagatataa cgatcgctgt cggtgataaa agcctaacaa tacttaatac
    60541 gttagctgca acaatcgtgg ttgatactca gcttggaaaa gcaatacaag aaggtttgaa
    60601 tttatttacg cgtggtgatt ggccagtatt gcagcctgaa tggaatcagg taaaaatatc
    60661 cggaaaattt aaagaagtgc ggttttggaa taggagtgtg tatctgtgac gcaagaattt
    60721 atttatgctt ataaaaaaat gccagatgat ttgagcgtga acggagcgtc gttagttgat
    60781 tgggaagatt tgccagaaat caatcgtgtg ctgaatggcc aatatcgttt ttatggtaac
    60841 tattcaagaa gcggacagta tcgctcttat ttaaaaaaag gaaattttat aaaagcgaag
    60901 gtaccagatg gatcatggca atattttgaa atttataatg tcaaaaagaa tttgacctcc
    60961 gtttctgtaa cagctcggca tattggtttt atggccaata agaactttat tgtcaagtcg
    61021 tttacggata acggcaatgg gtctcagatc atgaccaatc ttaaaaacag tttggcattt
    61081 gatcaaaaat ttaactatct ttcgaatgta gggacaacgc accaatttac agcaagacaa
    61141 gtcgcgccag ttgaagcaat cattggttca aataacggta atcaaaattt gactggcgtg
    61201 actagcgcgg aacttgatat ggataactat gatttgaaac tggtaaaaca gattggatca
    61261 gataatggtt ttcggattga ttttggcatc aatttagaag caatcgaaga agaaattgat
    61321 gaagaatcaa ttgtcaatag tttgtatttg gttggcggtg ttcctgataa cgattatgat
    61381 gaagacaaag aacctatcga atatggttat ttagaaattg atggagtaac caacgagaat
    61441 cgacgtattg ccaaacgtga aaacggagat tgtaaaacag ttgatgaatt gatcaaatgg
    61501 ggcaaaacct tatttgacaa tgatcgtatc catgagccaa aagctactca tacagtcagt
    61561 atggtggcgt tggaacacac actcgaatac ggtgacatgt atcaagagct agcgtcttta
    61621 cactttggtg atgtcgctca tgttagggcg aaagagctag atatcgagat aaaagagcgt
    61681 atggtcgaat acacttattt cccaacgctt gggaaatata aggatattgt tttaggtaat
    61741 gatttatcac tgtacacttc ggcagtaaat actcaagcac aagaattgaa aaagaaaatc
    61801 gacaatcgaa cagaaacgtt agttcaaaat gtgttgaatg cgacggcatg gatcactggt
    61861 aattctggtg ggcatgtcgt ttttcgtcca gaaaaagctc catcagagat tcttatcatg
    61921 gatactccaa atgtggcgaa tgcaaagcga gtgtggcgtt ggaatttaaa tggcttaggt
    61981 tattcagaca acggagtaaa tggcccgttc ggtattgcta tcacatcaaa aggagaaatt
    62041 gttgctgact tcatcaaagt aggaacaata aatgcagaag tattcgaaac ttcctttaat
    62101 gcttatggtg atgtgttaaa acttgtaaaa ggcacgcttc aaatttggaa tgaaaacaaa
    62161 aaaatcatgg agctaaccaa aaaaggtatg gaattctgga atagaaaaga atccattgga
    62221 acgatcggaa caacagattc agcaggaaat ccatttccaa atgctgtaac acctacacct
    62281 cttgaggaaa attctttggt aatacgtaca aatggcgatg gaaaatacat tttaatttct
    62341 ccaactgcag aaaaaggatt tgtgttatta ggaaacggaa aagcatatta tttcggtgat
    62401 ttagatattc agggaaaact tactgttaga ggtaaagaag tgataccagg acaaaatggc
    62461 ggtccatctg gcggaggaga aacacctggt gggtatcctg atgaattaaa aacagatgca
    62521 gaaaagagag cttggagaat
//
LOCUS       DAFELO010000006        60914 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFELO010000006
VERSION     DAFELO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.006"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(34..615)
                     /locus_tag="LP452_000470"
     CDS             complement(34..615)
                     /locus_tag="LP452_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2013226.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(748..1431)
                     /gene="rpiA"
                     /locus_tag="LP452_000471"
     CDS             complement(748..1431)
                     /gene="rpiA"
                     /locus_tag="LP452_000471"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL2013227.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1750..2163
                     /gene="rpsL"
                     /locus_tag="LP452_000472"
     CDS             1750..2163
                     /gene="rpsL"
                     /locus_tag="LP452_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL2013228.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2247..2717
                     /gene="rpsG"
                     /locus_tag="LP452_000473"
     CDS             2247..2717
                     /gene="rpsG"
                     /locus_tag="LP452_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL2013229.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2811..4898
                     /gene="fusA"
                     /locus_tag="LP452_000474"
     CDS             2811..4898
                     /gene="fusA"
                     /locus_tag="LP452_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL2013230.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5070..6257
                     /gene="tuf"
                     /locus_tag="LP452_000475"
     CDS             5070..6257
                     /gene="tuf"
                     /locus_tag="LP452_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL2013231.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6664..6972
                     /gene="rpsJ"
                     /locus_tag="LP452_000476"
     CDS             6664..6972
                     /gene="rpsJ"
                     /locus_tag="LP452_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL2013232.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7003..7632
                     /gene="rplC"
                     /locus_tag="LP452_000477"
     CDS             7003..7632
                     /gene="rplC"
                     /locus_tag="LP452_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL2013233.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7660..8283
                     /gene="rplD"
                     /locus_tag="LP452_000478"
     CDS             7660..8283
                     /gene="rplD"
                     /locus_tag="LP452_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL2013234.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8283..8573
                     /gene="rplW"
                     /locus_tag="LP452_000479"
     CDS             8283..8573
                     /gene="rplW"
                     /locus_tag="LP452_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL2013235.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8612..9445
                     /gene="rplB"
                     /locus_tag="LP452_000480"
     CDS             8612..9445
                     /gene="rplB"
                     /locus_tag="LP452_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL2013236.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9488..9766
                     /gene="rpsS"
                     /locus_tag="LP452_000481"
     CDS             9488..9766
                     /gene="rpsS"
                     /locus_tag="LP452_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL2013237.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9789..10136
                     /gene="rplV"
                     /locus_tag="LP452_000482"
     CDS             9789..10136
                     /gene="rplV"
                     /locus_tag="LP452_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL2013238.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10150..10806
                     /gene="rpsC"
                     /locus_tag="LP452_000483"
     CDS             10150..10806
                     /gene="rpsC"
                     /locus_tag="LP452_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL2013239.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10809..11243
                     /gene="rplP"
                     /locus_tag="LP452_000484"
     CDS             10809..11243
                     /gene="rplP"
                     /locus_tag="LP452_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL2013240.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11233..11421
                     /gene="rpmC"
                     /locus_tag="LP452_000485"
     CDS             11233..11421
                     /gene="rpmC"
                     /locus_tag="LP452_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL2013241.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11446..11712
                     /gene="rpsQ"
                     /locus_tag="LP452_000486"
     CDS             11446..11712
                     /gene="rpsQ"
                     /locus_tag="LP452_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL2013242.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11770..12138
                     /gene="rplN"
                     /locus_tag="LP452_000487"
     CDS             11770..12138
                     /gene="rplN"
                     /locus_tag="LP452_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL2013243.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12175..12483
                     /gene="rplX"
                     /locus_tag="LP452_000488"
     CDS             12175..12483
                     /gene="rplX"
                     /locus_tag="LP452_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL2013244.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12511..13050
                     /gene="rplE"
                     /locus_tag="LP452_000489"
     CDS             12511..13050
                     /gene="rplE"
                     /locus_tag="LP452_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL2013245.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13069..13254
                     /locus_tag="LP452_000490"
     CDS             13069..13254
                     /locus_tag="LP452_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL2013246.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13290..13688
                     /gene="rpsH"
                     /locus_tag="LP452_000491"
     CDS             13290..13688
                     /gene="rpsH"
                     /locus_tag="LP452_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL2013247.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13720..14256
                     /gene="rplF"
                     /locus_tag="LP452_000492"
     CDS             13720..14256
                     /gene="rplF"
                     /locus_tag="LP452_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL2013248.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14417..14773
                     /gene="rplR"
                     /locus_tag="LP452_000493"
     CDS             14417..14773
                     /gene="rplR"
                     /locus_tag="LP452_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL2013249.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14794..15294
                     /gene="rpsE"
                     /locus_tag="LP452_000494"
     CDS             14794..15294
                     /gene="rpsE"
                     /locus_tag="LP452_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL2013250.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15309..15488
                     /gene="rpmD"
                     /locus_tag="LP452_000495"
     CDS             15309..15488
                     /gene="rpmD"
                     /locus_tag="LP452_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL2013251.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15532..15972
                     /gene="rplO"
                     /locus_tag="LP452_000496"
     CDS             15532..15972
                     /gene="rplO"
                     /locus_tag="LP452_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL2013252.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15972..17267
                     /gene="secY"
                     /locus_tag="LP452_000497"
     CDS             15972..17267
                     /gene="secY"
                     /locus_tag="LP452_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL2013253.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17329..17976
                     /locus_tag="LP452_000498"
     CDS             17329..17976
                     /locus_tag="LP452_000498"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL2013254.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18167..18385
                     /gene="infA"
                     /locus_tag="LP452_000499"
     CDS             18167..18385
                     /gene="infA"
                     /locus_tag="LP452_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL2013255.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18418..18534
                     /gene="rpmJ"
                     /locus_tag="LP452_000500"
     CDS             18418..18534
                     /gene="rpmJ"
                     /locus_tag="LP452_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL2013256.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18552..18917
                     /gene="rpsM"
                     /locus_tag="LP452_000501"
     CDS             18552..18917
                     /gene="rpsM"
                     /locus_tag="LP452_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL2013257.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18945..19334
                     /gene="rpsK"
                     /locus_tag="LP452_000502"
     CDS             18945..19334
                     /gene="rpsK"
                     /locus_tag="LP452_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL2013258.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19414..20352
                     /locus_tag="LP452_000503"
     CDS             19414..20352
                     /locus_tag="LP452_000503"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL2013259.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20380..20760
                     /gene="rplQ"
                     /locus_tag="LP452_000504"
     CDS             20380..20760
                     /gene="rplQ"
                     /locus_tag="LP452_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL2013260.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21032..21871
                     /locus_tag="LP452_000505"
     CDS             21032..21871
                     /locus_tag="LP452_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2013261.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21847..22716
                     /locus_tag="LP452_000506"
     CDS             21847..22716
                     /locus_tag="LP452_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2013262.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22706..23503
                     /locus_tag="LP452_000507"
     CDS             22706..23503
                     /locus_tag="LP452_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL2013263.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23706..24452
                     /gene="truA"
                     /locus_tag="LP452_000508"
     CDS             23706..24452
                     /gene="truA"
                     /locus_tag="LP452_000508"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL2013264.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24785..27454)
                     /locus_tag="LP452_000509"
     CDS             complement(24785..27454)
                     /locus_tag="LP452_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL2013265.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(27692..27880)
                     /locus_tag="LP452_000510"
     CDS             complement(27692..27880)
                     /locus_tag="LP452_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL2013266.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28150..28512
                     /locus_tag="LP452_000511"
     CDS             28150..28512
                     /locus_tag="LP452_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2013267.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            28564..30246
                     /locus_tag="LP452_000512"
     CDS             28564..30246
                     /locus_tag="LP452_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL2013268.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            30363..32399
                     /gene="recG"
                     /locus_tag="LP452_000513"
     CDS             30363..32399
                     /gene="recG"
                     /locus_tag="LP452_000513"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL2013269.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            32448..33449
                     /gene="plsX"
                     /locus_tag="LP452_000514"
     CDS             32448..33449
                     /gene="plsX"
                     /locus_tag="LP452_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL2013270.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            33571..33813
                     /gene="acpP"
                     /locus_tag="LP452_000515"
     CDS             33571..33813
                     /gene="acpP"
                     /locus_tag="LP452_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL2013271.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34081..35085
                     /locus_tag="LP452_000516"
     CDS             34081..35085
                     /locus_tag="LP452_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013272.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35086..36030
                     /locus_tag="LP452_000517"
     CDS             35086..36030
                     /locus_tag="LP452_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013273.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36030..36992
                     /locus_tag="LP452_000518"
     CDS             36030..36992
                     /locus_tag="LP452_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013274.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37019..37933
                     /locus_tag="LP452_000519"
     CDS             37019..37933
                     /locus_tag="LP452_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013275.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            37959..39740
                     /locus_tag="LP452_000520"
     CDS             37959..39740
                     /locus_tag="LP452_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2013276.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40088..40774
                     /locus_tag="LP452_000521"
     CDS             40088..40774
                     /locus_tag="LP452_000521"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL2013277.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            40794..44375
                     /gene="smc"
                     /locus_tag="LP452_000522"
     CDS             40794..44375
                     /gene="smc"
                     /locus_tag="LP452_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL2013278.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            44384..45193
                     /locus_tag="LP452_000523"
     CDS             44384..45193
                     /locus_tag="LP452_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2013279.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            45204..46202
                     /gene="ftsY"
                     /locus_tag="LP452_000524"
     CDS             45204..46202
                     /gene="ftsY"
                     /locus_tag="LP452_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL2013280.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            46389..48656
                     /gene="gshAB"
                     /locus_tag="LP452_000525"
     CDS             46389..48656
                     /gene="gshAB"
                     /locus_tag="LP452_000525"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL2013281.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(48745..49197)
                     /locus_tag="LP452_000526"
     CDS             complement(48745..49197)
                     /locus_tag="LP452_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL2013282.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            49347..50297
                     /locus_tag="LP452_000527"
     CDS             49347..50297
                     /locus_tag="LP452_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL2013283.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGMIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            50290..51249
                     /locus_tag="LP452_000528"
     CDS             50290..51249
                     /locus_tag="LP452_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL2013284.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            51246..52001
                     /locus_tag="LP452_000529"
     CDS             51246..52001
                     /locus_tag="LP452_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2013285.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52024..52977
                     /locus_tag="LP452_000530"
     CDS             52024..52977
                     /locus_tag="LP452_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2013286.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(53621..53917)
                     /locus_tag="LP452_000531"
     CDS             complement(53621..53917)
                     /locus_tag="LP452_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL2013287.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            54272..54628
                     /locus_tag="LP452_000532"
     CDS             54272..54628
                     /locus_tag="LP452_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2013288.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            54638..55537
                     /locus_tag="LP452_000533"
     CDS             54638..55537
                     /locus_tag="LP452_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL2013289.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56053..56784
                     /locus_tag="LP452_000534"
     CDS             56053..56784
                     /locus_tag="LP452_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2013290.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            56929..58956
                     /locus_tag="LP452_000535"
     CDS             56929..58956
                     /locus_tag="LP452_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL2013291.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            59064..60161
                     /locus_tag="LP452_000536"
     CDS             59064..60161
                     /locus_tag="LP452_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL2013292.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            60468..>60914
                     /locus_tag="LP452_000537"
     CDS             60468..>60914
                     /locus_tag="LP452_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013293.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        20167 a        10903 c        13007 g        16837 t
ORIGIN      
        1 aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct cgatttcttt
       61 ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt ttgaagaaat
      121 aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga tcgtttttgc
      181 atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta atccaactaa
      241 tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta attggaaata
      301 gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg
      361 atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta atcgatgaag
      421 ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact ccaaatgaac
      481 agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt gacaaccaaa
      541 gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg tgcgtgcata
      601 accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga tataacagta
      661 aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc gctgtctttt
      721 tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa gaatctcaac
      781 gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc catgttctac
      841 taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga tgacttctag
      901 atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt tttcgtctgt
      961 acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt gggagccata
     1021 agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt tagaagaatc
     1081 aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc
     1141 gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt ctattgtcaa
     1201 atcgactacg gggacttcat caatactctt taacggtatt cctaattttt ccgcttgttc
     1261 ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac gcatacggcg
     1321 accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta cgatcatccc
     1381 gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca tctttgcgtc
     1441 cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa
     1501 aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca tctcttgttg
     1561 acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt ttctgtgaag
     1621 aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg catttatttt
     1681 ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt gggaaggagg
     1741 aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa
     1801 aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac tcaaactaac
     1861 gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac acctaaaaaa
     1921 ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat tgaagttact
     1981 gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt attacgcggt
     2041 ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc tttagatact
     2101 gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa acctaaagct
     2161 taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa ttactatgaa
     2221 caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg
     2281 ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg
     2341 ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac ttgatcaaag
     2401 aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg
     2461 ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca gttgaagttc
     2521 gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg
     2581 gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca aacaacacag
     2641 gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac cgtgcgtttg
     2701 ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg acctcagata
     2761 gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga atgaccagag
     2821 aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa
     2881 cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt ggtgaaactc
     2941 atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt attacgatca
     3001 cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc gacacaccag
     3061 gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa
     3121 ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa
     3181 caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc ggtgctgact
     3241 tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac ccaatccaat
     3301 taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca atgaaggctg
     3361 aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct gaagaatacc
     3421 gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag
     3481 aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta aaagaaggta
     3541 tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca gcatttaaga
     3601 acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca ccgttagata
     3661 tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg
     3721 acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc gtaggtcgtt
     3781 taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta caaaacgcaa
     3841 caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac tctcgttcag
     3901 aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa gacacaacta
     3961 ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg gaattcccag
     4021 aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat aaaatgggtg
     4081 tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca aacgtggaaa
     4141 caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc gttgaccgta
     4201 tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa
     4261 ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc
     4321 aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt ttcgaatttg
     4381 aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt gaaactggtt
     4441 tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat atcaaagcta
     4501 aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc cgtgttgctg
     4561 cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt gaaccaatca
     4621 tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt cacgtaactg
     4681 ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg
     4741 cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct gctactcaag
     4801 gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa tcagtacaag
     4861 aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct
     4921 gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa ataattatcg
     4981 atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca aataaaaaat
     5041 atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac cgttctaaac
     5101 cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca ttaacagctg
     5161 caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac gatcaaatcg
     5221 atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac gttgagtatg
     5281 aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat tacgttaaaa
     5341 acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct gctgctgacg
     5401 gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt gttccttaca
     5461 tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta gaattagttg
     5521 aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat gttcctgtag
     5581 ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa aaaattcttg
     5641 aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac gacaaaccat
     5701 tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt gctacaggtc
     5761 gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt attgctgaag
     5821 aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta gactacgctg
     5881 aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac atccaacgtg
     5941 gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct gcagaagtat
     6001 acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac taccgtccac
     6061 aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa ggaactgaaa
     6121 tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca atcgctatcg
     6181 aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta
     6241 cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat tcggacgtaa
     6301 tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat tgcaggatca
     6361 ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt cagtattttc
     6421 taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc tttgtgagtt
     6481 tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga cgtaatcaga
     6541 atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt gccacgaacg
     6601 agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga aggagggaac
     6661 aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg tattttagat
     6721 caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt gtctggtcca
     6781 attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca taaatacaaa
     6841 gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca
     6901 acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt aaacatcgaa
     6961 atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa aggaatctta
     7021 gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat tccagtaact
     7081 gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac ggatggctac
     7141 gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa
     7201 ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt
     7261 gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca agcaggagac
     7321 attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat caaacgccac
     7381 ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg
     7441 ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc
     7501 aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc
     7561 ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa atcagctgtg
     7621 aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt agcattattc
     7681 aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt cggaattgaa
     7741 cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga
     7801 acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa
     7861 aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg tggaggtggc
     7921 gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa agttcgtcgt
     7981 ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa
     8041 ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc taacttatct
     8101 atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc tttatcagca
     8161 cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct agacgttgta
     8221 tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga ggtgcttgca
     8281 taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca
     8341 tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac
     8401 aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg
     8461 cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg
     8521 ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa taagcactaa
     8581 tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc
     8641 aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat caacacctga
     8701 aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg gccgcatcac
     8761 tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa
     8821 caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc gttcagctaa
     8881 catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac ctaaaggact
     8941 agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg gaaatgctct
     9001 tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga aacctggtaa
     9061 aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta aagaaggcaa
     9121 atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg catgccgtgc
     9181 tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta aagctggacg
     9241 ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc ctaatgatca
     9301 cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag tttctccttg
     9361 gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag acaaacttat
     9421 tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt ttgagaggag
     9481 gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt taatgaaaaa
     9541 agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc
     9601 aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac gtaaacatgt
     9661 accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg cacctacaag
     9721 aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg aggggaggat
     9781 aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt
     9841 cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa
     9901 tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt
     9961 cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg gtagtaagcg
    10021 aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca aaaggatcag
    10081 cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa aaataaggag
    10141 ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat catccgcgac
    10201 tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga agatttaaga
    10261 atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt tgaaatcgaa
    10321 cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat ggttattggt
    10381 aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac tggtaaaaga
    10441 atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa
    10501 ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc
    10561 caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac
    10621 ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct tcatacttta
    10681 cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa actaggagtt
    10741 aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga gaaaggaggg
    10801 aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc
    10861 gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg caagctgttg
    10921 attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg actcgttaca
    10981 tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat actgccaaag
    11041 caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt gcaccagtaa
    11101 aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt
    11161 tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg
    11221 gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga aatgcttgat
    11281 caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc aacaggtcaa
    11341 ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat caaaactgtg
    11401 ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac tgaaggaaga
    11461 aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa aacaattacc
    11521 gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa ctattctaag
    11581 aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt gagaattatg
    11641 gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca
    11701 gttattatct aataacacga gattttccta tatagattga atgcaaaatc tgaaaggagg
    11761 atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt
    11821 gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa catcggtgat
    11881 gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc
    11941 gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg ttcatatatt
    12001 aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg tggaacacgt
    12061 atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat cgtttcacta
    12121 gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac agttatgttt
    12181 gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa agaaggcgtt
    12241 gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa
    12301 aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat ggaagcgcca
    12361 attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg agttggctac
    12421 aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa
    12481 taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa atatcttaaa
    12541 gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca aacacctaaa
    12601 gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta
    12661 gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat tacaaaagct
    12721 aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc aaaagttaca
    12781 ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc actaccacgt
    12841 gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta
    12901 ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga taaagtacgt
    12961 ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg tgagttgttg
    13021 acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa
    13081 tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta tactcgttgc
    13141 gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg tatttgcttc
    13201 cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta
    13261 aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt gcagattttc
    13321 taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta cctgcatcaa
    13381 aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat
    13441 acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg
    13501 aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag
    13561 ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg acttctgaag
    13621 gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg
    13681 tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat cggaaataaa
    13741 gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt tacagttaaa
    13801 ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa tatcgaagga
    13861 aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca
    13921 actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca aaaagcgtta
    13981 gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt
    14041 ggttactcac atccagtaga aatcacacca ccagctggag taactgttga agtaccagct
    14101 aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac
    14161 attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta tgttggcgaa
    14221 tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat tgcatagaag
    14281 ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac atttttactt
    14341 gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt acaataaaaa
    14401 taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc aaaagagaca
    14461 tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga acgtttttcg
    14521 ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag
    14581 tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag cacaagctgt
    14641 cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag tctttgaccg
    14701 tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc gcgaaaatgg
    14761 actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc aaaacatttg
    14821 gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt
    14881 cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt agggtttggt
    14941 acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga tgcgaagaaa
    15001 aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat cggtgctttt
    15061 agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc tgctggtgga
    15121 ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa atctttaggc
    15181 tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca attgaaacgt
    15241 gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg ataaggagga
    15301 caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga cgtcctcaaa
    15361 accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca gtggttaagc
    15421 cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg gacgttgaag
    15481 aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc tatgaaactt
    15541 catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg tcgtggtact
    15601 tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc
    15661 ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg tttaccaaaa
    15721 cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga tgtattgaac
    15781 cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg aatcgtgaaa
    15841 aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa attaactgtg
    15901 aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg tggatcaatc
    15961 gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac attagatcga
    16021 aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat attactgtac
    16081 caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc aacatgttga
    16141 acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg gtttcaccat
    16201 acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct cgatttgttg
    16261 aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact cgatatctga
    16321 cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat caatacgctc
    16381 aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt ttgattctaa
    16441 cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa ggtatcggaa
    16501 atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa agtatcagag
    16561 aaatttatga agattacttt ataaatattg atccttcaga catatggaaa tctgctattt
    16621 ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca tttttccaac
    16681 aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg cctacaagca
    16741 gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt gctagttcgt
    16801 ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt tctgaaaact
    16861 ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct acgatttata
    16921 cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac cctgagaaat
    16981 tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct ggtaagggga
    17041 ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca gtcttccttg
    17101 gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca caatcaatcg
    17161 gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt
    17221 tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa agcgtgttga
    17281 ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat gaacctcatt
    17341 ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat tgatgcttat
    17401 ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct
    17461 cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga tgaagtaaca
    17521 aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt cttattagat
    17581 ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa agatttgaat
    17641 aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt cgaacgtcta
    17701 gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt caaccctaca
    17761 aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag agaagatgat
    17821 aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga accaatcttg
    17881 gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga aatcgatgct
    17941 gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta gccgacttgc
    18001 attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca gtccgacttc
    18061 tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc agtttccgcc
    18121 attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga
    18181 tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt ttaaagttga
    18241 actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta tgcattacat
    18301 ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg
    18361 ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg tattatcatg
    18421 aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg tcgtaaagga
    18481 cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg ataattggag
    18541 gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa cgtgtagtag
    18601 tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc ttagctgatg
    18661 ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc
    18721 gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca
    18781 tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt ggattgcctg
    18841 ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct cgcgctatcg
    18901 ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag
    18961 tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat atccattcta
    19021 cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta gcgtggtcat
    19081 cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg
    19141 cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt gaagtaacag
    19201 ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct acaggtttag
    19261 aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc cctccaaaac
    19321 gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc attgaacaag
    19381 acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa accaagaatc
    19441 gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct tgaaagaggg
    19501 tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt gccaggtgct
    19561 gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat caaaggtgtt
    19621 agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa aatgtatggc
    19681 caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac tgctggcgat
    19741 attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg tagtgtctct
    19801 gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca
    19861 gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc aatctacaca
    19921 cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg tgaagatttt
    19981 gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga agcaatgagt
    20041 ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac tgatgaagca
    20101 aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg
    20161 acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc
    20221 aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt ccgtaactta
    20281 ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt aggtttacgt
    20341 aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg taaactagga
    20401 cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt attaatcaac
    20461 gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg
    20521 atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt
    20581 aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc agctttacaa
    20641 aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata cacacgcatt
    20701 ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa
    20761 aatcacacta ataacatcac tttatagatg attcttttag agtgttatga tgttggaaga
    20821 tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca
    20881 ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata tgtttgaagg
    20941 cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt gatagtgtta
    21001 gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat tagaaaagat
    21061 aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct cctttacaat
    21121 tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat cgacacttgc
    21181 caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat
    21241 attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatggttt ttcaaaatcc
    21301 agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc ttgaaaatca
    21361 agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga aagttagaat
    21421 ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac aacgtgttgc
    21481 cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag caaccagtat
    21541 gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca aagaagaaag
    21601 tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg ccaatcggat
    21661 actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc
    21721 tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac tgaagagcgc
    21781 actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa ggatggtgga
    21841 ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa tacaccattt
    21901 gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta tactgcatta
    21961 gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc gcttgttaag
    22021 ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac ggataataag
    22081 aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga agcacagcta
    22141 ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg tgtcagcgaa
    22201 gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga tgaaagctat
    22261 ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc aatcgctggt
    22321 gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg acttgatccc
    22381 cagggcagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca gcagatcact
    22441 attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta tgtctatgtg
    22501 ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca aaacattgaa
    22561 tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga agaattaatg
    22621 gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc agacgctatc
    22681 gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc gttatatgcc
    22741 tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca gtttttattt
    22801 tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg catttttcac
    22861 attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag gcattcgccc
    22921 attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga
    22981 aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga acggcctctt
    23041 tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc ttactacacc
    23101 gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag ccgtacggtt
    23161 cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc ctacattgat
    23221 ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg
    23281 aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat ttgtcagcag
    23341 cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca aaggtgggga
    23401 aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg ttatcattgc
    23461 ttttgtatta ttaactgtgc tactgatctt tttaagagga taaacgttca gtgggccgct
    23521 atctattctt ttcttgctga tctgtttgca agaaaggatg caagcagtgt atgacaagaa
    23581 atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg aaaagaaaga
    23641 acgaaaaaat tttatagaaa aatcattatt ccagataacc ttttaggaac aaaggagaga
    23701 aaaacatggt acgttacaaa gcaatcattg cgtacgacgg aacaaatttt aatggattcc
    23761 aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta caaaaaatgg
    23821 caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga gtacatgcga
    23881 tcgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga atgcgtttcg
    23941 cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt gtttcagaag
    24001 acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg gatataggga
    24061 aaccacgcag tccttttcga cgccattatg caagttattt tccttaccca cttgatttgg
    24121 aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc tctttttgtg
    24181 catcaggaag ctcgatagag gataaaatac gtacgatcta cgaggctaag atggaggtaa
    24241 atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg tataagatga
    24301 tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct gaagatagta
    24361 tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca caccctgaag
    24421 gactttattt gtatgaagtg aaatatgaat aagaggttat gatagaagtg gtcagcttca
    24481 agaaataaga aggtatttac gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt
    24541 ttcgaagaag ctacttctgg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    24601 tctgagtcac aaagagcaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    24661 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    24721 aaacgatgga acgttgtttt gattgccctt ggtgcggaca gtctgaacag gttccatcgt
    24781 ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg ttgttcttct attgctggat
    24841 caatcgtgcc ataaaagggg cgattgcggt caaggtacca gacataaaga ataaacaatg
    24901 caatacatag gacaaaaagg attgcaccta tcgtaaatcc ttcaggcaag taactgaaag
    24961 tgacagtatg tttacctgct ggaactttta tcatcaagaa tgcgtctttg aaattgctga
    25021 ccttgactgg tttcccatca attttagctt tccatccttt gtcataagga atagttgtca
    25081 ccaacatgga atcttcagcg gtatttacag tcccagaagc ttttctgcca gtggcttcca
    25141 tttcgacacc tttttctttg atctgattca ttgcggcttg ataggctaca acatctagac
    25201 caacgacttt aggctccatg aaacttattt ctttcgtccc ataaaaactt gcagtaaatg
    25261 tcactgttgt ctcttgagga taatagccaa gatcatagta ttgaccagaa atgttgattt
    25321 gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact agaactttct aattgcgcaa
    25381 aattggttgg gaacaaactt aagtaagctt gtgtatttgc aggcacatgg acttcccaag
    25441 tgagctcttt tggctggtta tctgctgttt ctgagtaagt gacgccagtc gttgtctttg
    25501 tgatcgtcgt attcaactga ttcttcaaag taggtgtata gaatgtaaaa tactgttcat
    25561 ttagaccaga caatgtattg atcaagtttg tctgactggt caaattatct gtcactggct
    25621 gctcaacatc attaacggac aacggtgcag taaatgctag tggcaatgca ttgacattct
    25681 gatacagtgt atagtcgccg ctttgaccag cttggcggaa gccatattta ctgaacgtgc
    25741 tttcgttttt agaaatgtta tatttgattc ctgtaaaccc atccatcagc aaagtattat
    25801 tgttataacg gatattcaaa tttgtccctc ttgaacggaa acctaattta tccaggtaag
    25861 aggaagaatt acgatttctt atagaagaaa acaaactgat accactgtaa ccatagttga
    25921 tcgaatcgtt tgcagatact ggatccagat tttccaagcg gtaaaacgag tcggaatctt
    25981 tttttgtttc atttacaaga gctttcaagt caggataagg atcagtatat aaactgcggg
    26041 aagcataatt ccaatcatca agaatgccat ggatcatact gttcgtattg accagtgctt
    26101 cagcagacat gatcaataat aacaatatac tcagatgatt gaatgaaagt tttttatgct
    26161 gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac cactgtcaat acaaaggaag
    26221 tcatcgtcac ataggtgtag cttccttcag gtttgatgcc ccatgccacc gcaaagagag
    26281 cagctacgac caaaatgatt cctaacaaca gcccacgatc gtcttgttct atcttttccc
    26341 atccttttcc tgcaagcagg agcactaaga aagagaacag ataggcataa cggaataaaa
    26401 acatattcgg tgcatgcata ccatgccaga acaaattcaa tggaacaaga taaaaacttg
    26461 cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt tttcaacggt actttccgac
    26521 tggcaaaata aaacagacag aggactaatg gtagcagacc tgcgtaaacg aacggaatcg
    26581 atccgtattt tgtcgtgtca taaacaccaa tcatattttt catgattaga tccagataag
    26641 aagtcgcttc tgttttcaac gtagtgatct gactcagtgc ctcgccattt gaccgaagat
    26701 ccagcactgc tggcaataca atgatcattg aagcgccgcc tgccagtaaa gaagtgatac
    26761 cataaggaac aatcgttttt ttatattctg accagtttgt caatagacgg gcgataaaat
    26821 ataagaaaga aaatacgcca atcataaaac ccatataaaa actggataaa aacaagagta
    26881 aataactgat aaataaaagt ttcggccgtt taagatccat aacacgatga atccccaaaa
    26941 tcactaatgg aagataaaca aatgcatcca gccacatgat gatctccgaa tgtgcagtga
    27001 taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg cccccagcga ggaatcttga
    27061 acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc gaccttaagc aaagtcagaa
    27121 catacagtgc atctggcatc atctggtttg gaaaaaagaa caccagtggc gtgaagatcc
    27181 cgcctagata gtaagaaatt aaagataaat aattcaatcc tagagaagca ttccatgtat
    27241 aaaaaatact ttgctttcca agaagaacat ttcggaagct ggcatgaaaa ttagaaaact
    27301 gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc aggatagatt ccgatactta
    27361 gataaacaag tgctaaaatc aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt
    27421 tttctctaaa gaatgacttt aaattttttt tcatatttat tttccaccaa atttctatga
    27481 agttgtattc agtttatcat atccttgttt ttctctgctt aaaaagagtc tagttttcac
    27541 aaaaaaataa aatttagaat aaataacttc ataatttgtt cgtataacaa aaataaacgc
    27601 aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt
    27661 tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt cctgatttca
    27721 aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg cgaacttttt
    27781 gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg ttgtttccac
    27841 tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc tcctttgcct
    27901 tgatttggag caatagaata attcagctca taccagacta acatatcata tagaatggga
    27961 atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt gtaaattaaa
    28021 caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg ccttttattt
    28081 caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac ccgttaagga
    28141 ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat taccaatgat
    28201 gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc
    28261 agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa ttactcaaga
    28321 ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac gattatcagc
    28381 tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa atacaatcta
    28441 gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg tgttcgtgta
    28501 ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag gaggattatc
    28561 taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg
    28621 aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc tgtgccagat
    28681 ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc agtagtagat
    28741 tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt gctgatgggt
    28801 gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc taagcaaatt
    28861 ccagatgttg tctcattaaa tgcttctgat cttgctgcag ccttcactca tggagttgaa
    28921 actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt agctagagaa
    28981 gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga agtaatgaca
    29041 gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc cagacttgct tccagtccta
    29101 aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta tgagggcttt
    29161 ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc tccagcagaa
    29221 atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa
    29281 gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg acctacagtc
    29341 gatagtacat tcgattacga agaattccgt aactatttag atggtatagg agattcttta
    29401 cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa
    29461 gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga caacatgcgc
    29521 ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa
    29581 ataccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg
    29641 tttgaaagtt taggcgctaa ttatgttatc agtggtggac aaacaatgaa tccaagtacg
    29701 gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt cttgccaaac
    29761 aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat cccagtagca
    29821 gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt caatgatcaa
    29881 gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt
    29941 caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa agaaggagac
    30001 tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac agcaacagcg
    30061 ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat cttggcaggt
    30121 gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga aaaacatcct
    30181 gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct attttctgct
    30241 gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa aggccttcgg
    30301 ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat
    30361 ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa aaaagagcag
    30421 ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat tatccttttc
    30481 gctacgaaga tattcaggaa agaaatctaa acgagatcca ggatcaagaa aaggttactt
    30541 tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa aaaagccgat
    30601 tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt aatcaacctt
    30661 atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa
    30721 agcgtaaagc cttgaacggg atgaagattt taggttctca aacaatggat gacttttcgc
    30781 cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta atcaaaaaag
    30841 ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt gtggagaaat
    30901 atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag
    30961 aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc
    31021 ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc ctttatgatg
    31081 tccaaaaatt gaaacaattt actcaaaagc taccttttga actgacaaat gcacaaaaac
    31141 gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag cgacttttgc
    31201 agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta
    31261 cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa caacatatgg
    31321 aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgatt acggggtcaa
    31381 cgaaagcaaa agaacgacgt gagctccttg aaaaactaga gcaaggggaa attaacgtcg
    31441 tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta ggtctagtta
    31501 ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt gaaaaaggct
    31561 ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta gctattacag
    31621 catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt atcccgatcg
    31681 aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg aaaaaagaac
    31741 tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca gaagcattag
    31801 atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca ccaaaatatg
    31861 aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc atggaagcct
    31921 tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta ggggtaaacg
    31981 tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg gcacagctgc
    32041 accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta
    32101 atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc accaatggtt
    32161 ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt ggcttccgtc
    32221 aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat gttttggagg
    32281 tagccagaga agaggctgca gccctttggc aaataaagga ttggcagatg cttcctgagt
    32341 atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat ttcgattagc
    32401 agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg
    32461 tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg gctttagcgc
    32521 agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa atcaagaaat
    32581 acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc aatagtgatg
    32641 atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt gctgcacaag
    32701 cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc gcgctactag
    32761 cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc ttgatgtcta
    32821 ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt ggcgctaacg
    32881 cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt tatgcacgta
    32941 aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa
    33001 aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca agcttgaatt
    33061 ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta gtggtcacag
    33121 atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg aatatgatga
    33181 atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt
    33241 taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat ggcggtgccg
    33301 ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc cccgatgctg
    33361 ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt ggacaattag
    33421 tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcgat ccgttccatt
    33481 gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg taggtaattg
    33541 acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta
    33601 gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga tgaattgaac
    33661 atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt agagcttgaa
    33721 gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac agtcggtggc
    33781 gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa
    33841 aagctgcatg cagcttttta tccttctttt tttaatcttt ttgacacgta aaagtcattt
    33901 ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcggt tttctatata
    33961 attattaata attcattaca caaagataag aataaccttt gttataaaac gatttattct
    34021 gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag gggtattcga
    34081 ttgtccgata accaattatt agaagttcag aacctacata caggattccg actcaaagat
    34141 gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga gattttagca
    34201 atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat gggattactt
    34261 gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa tttaatcgac
    34321 ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat tttccaagat
    34381 ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga aggactttcc
    34441 tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt gctagaacaa
    34501 gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact atcaggcggt
    34561 atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat cgtcattgca
    34621 gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga tttactgaaa
    34681 gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt aggagtcgta
    34741 gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga agtagcaggt
    34801 gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact gaactctata
    34861 ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt tccttctctg
    34921 aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat accagcaagc
    34981 gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt acgttgctcc
    35041 tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac agaattaatc
    35101 acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa tcgagtaacg
    35161 gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa gacatatggt
    35221 ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt gggattagaa
    35281 aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc
    35341 aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat gtccagtttg
    35401 aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt tgaacggtta
    35461 agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg gatgccggaa
    35521 gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg tctaggagta
    35581 gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt atctgctctg
    35641 gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca agaatacggc
    35701 ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg cgacaatatc
    35761 gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat ttataatgac
    35821 ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga tgtagaaaat
    35881 agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa
    35941 gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac tacacacaaa
    36001 gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga cgtgttttat
    36061 tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc caagcgatgc
    36121 cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg atcgaacaga
    36181 tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt tggatcggaa
    36241 atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt gctgacttga
    36301 tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc cttacttatt
    36361 tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc ttggataaga
    36421 ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc ggcttgatca
    36481 tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca caaacactag
    36541 gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt ctgttaccat
    36601 caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga agtgaagtaa
    36661 tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta ccgacgaata
    36721 aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct caaatggggt
    36781 atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt ggttatccgg
    36841 gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg atcacatcct
    36901 tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat atcatcatga
    36961 gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga atggatcgat
    37021 gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga tacgaatgat
    37081 tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc tagtggtttt
    37141 attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag
    37201 tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa agttcattct
    37261 gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg ctagaaactc
    37321 tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg gattagggat
    37381 catctctggc tactacggag ggatcattga caacatcttg atgcggatcg ttgattttat
    37441 catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt ccagatacaa
    37501 tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag cccgattatt
    37561 ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa aaacaatggg
    37621 aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt cattaatcat
    37681 tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt tgactttctt
    37741 aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt atgcaagtaa
    37801 cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc ttattttagt
    37861 tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag atgcgcgcca
    37921 acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg atttttggat
    37981 ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt acagatacct
    38041 ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt ccaattgcaa
    38101 caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc gcaacggaca
    38161 gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat gcagctttta
    38221 tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc acagacgacg
    38281 gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca
    38341 acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc ccttacgaaa
    38401 tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt aacatcgtag
    38461 gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa aaaatagacg
    38521 acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa tcgggtggtg
    38581 gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc aaagatatgg
    38641 aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac atgagtaata
    38701 tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat ggtacgccta
    38761 aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa gcattgaact
    38821 ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc tataaagaaa
    38881 ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta ggattcaagt
    38941 taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca aaaatggctg
    39001 ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta ggtgaacgtt
    39061 tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc cagtcatacc
    39121 atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa ctattagatg
    39181 atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat ggaaataaac
    39241 taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg gcagattact
    39301 atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt cgtctgatcg
    39361 aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat gtttaccaag
    39421 ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca aatgctgcgt
    39481 tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat atcagttcaa
    39541 gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa caatatgcat
    39601 ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca gtcaacgacc
    39661 gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat gtttctgtta
    39721 cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg
    39781 ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg atttcatgac
    39841 ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa
    39901 atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac gaatttttca
    39961 caacttctcg tcaatcacta ggattattaa agctttactt accttttgac cgaaccattt
    40021 caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca taaaaggaat
    40081 gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca tcgtttttca
    40141 cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg agcatcgcaa
    40201 tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt tagaactgat
    40261 ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac
    40321 acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat gtcactttga
    40381 tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc gaccagcatt
    40441 gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg gattcgacac
    40501 gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca
    40561 tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg atgtatcaat
    40621 cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt
    40681 ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca
    40741 agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt
    40801 tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc attgattttg
    40861 aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat attacagagg
    40921 ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg
    40981 atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa gtcacagtga
    41041 ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt gtaacccgcc
    41101 gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc agattaaaag
    41161 atatccaaga attgttctta gattctggat taggaaaaga atcgttctca atcatttctc
    41221 aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg atctttgaag
    41281 aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag aagctttttg
    41341 aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa gaacagttaa
    41401 ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa gaaacactga
    41461 ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa gattgggaca
    41521 acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct gagtcgatcc
    41581 aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga
    41641 tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag acagaaggac
    41701 aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa
    41761 cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa gaaagcttga
    41821 tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa
    41881 cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca gaattaagag
    41941 atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg aagtatctag
    42001 aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag caatcggaag
    42061 tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa aagcaggcgc
    42121 agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa caaaacggga
    42181 aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta atgaatcaag
    42241 tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt
    42301 tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt attgtcggag
    42361 cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa acagctttag
    42421 gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt
    42481 atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca atcaaaccaa
    42541 gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt atagggattg
    42601 ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa
    42661 cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc
    42721 aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg
    42781 gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt
    42841 tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa aaagttcagg
    42901 agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt acacgaggcg
    42961 aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc ataaatgaac
    43021 ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa ctgcagcagt
    43081 ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca gatcttgaat
    43141 cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc gaccagatgg
    43201 aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat cttgctgtct
    43261 tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag
    43321 aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca gcagactttt
    43381 ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta tctgctcagc
    43441 gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga aggcaaaagg
    43501 agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt
    43561 cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta gataatcatt
    43621 tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa gactatcaag
    43681 aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa cagattgaaa
    43741 aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt gaaagacata
    43801 cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg tttgaaacga
    43861 tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa gctatccgtc
    43921 aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa ttagtactga
    43981 cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct cctggcaaaa
    44041 aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc atcgcgttgc
    44101 tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag gtagaagcag
    44161 cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc caagatgaca
    44221 cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat gtactgtacg
    44281 gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca
    44341 atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca aattagtagc
    44401 aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg agaataaaca
    44461 agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag gacgcccctt
    44521 agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg actacagcat
    44581 cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt
    44641 gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg atctgccact
    44701 agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga agcaatcctt
    44761 gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg atattacaga
    44821 tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag atcctaaaat
    44881 caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa gaagtatgct
    44941 tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat tggcaaaaga
    45001 tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg atttaccgat
    45061 gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc
    45121 agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt
    45181 tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg
    45241 gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga
    45301 cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg
    45361 aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag
    45421 cgatacgaat taccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg
    45481 cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca
    45541 atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg
    45601 ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag
    45661 gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag
    45721 ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag
    45781 cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat gttttactag
    45841 ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa
    45901 aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt gtattagatg
    45961 caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga
    46021 ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc
    46081 gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg
    46141 agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat
    46201 aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg
    46261 tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc
    46321 aaacaatttc gttctttttc ttagactttt tgattataat tagattcata tgaattggag
    46381 gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg
    46441 accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag
    46501 caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag
    46561 attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt cccgaattat
    46621 ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga
    46681 tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc attattgcaa
    46741 aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaagaa tacgggaagc
    46801 gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag ttgctaagaa
    46861 cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt
    46921 tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt
    46981 caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca
    47041 gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt
    47101 ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg
    47161 agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa
    47221 caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg
    47281 gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg ttatggacag
    47341 aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag
    47401 cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag
    47461 gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag
    47521 cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac
    47581 agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc
    47641 atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta
    47701 tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt
    47761 tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg
    47821 acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag
    47881 aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag
    47941 cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt
    48001 taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca
    48061 ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc
    48121 gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta
    48181 ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc
    48241 gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga
    48301 tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc
    48361 taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt
    48421 ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg
    48481 gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa aaagcatatg
    48541 ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct tttgcaggaa
    48601 aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga
    48661 aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt tttttgcatg
    48721 taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt
    48781 tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat
    48841 cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc
    48901 agtgacgtaa tcgttgacta cagtgaaacg gctattgctt ttcgttgcta gctggatata
    48961 ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt
    49021 tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat
    49081 actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac
    49141 taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa
    49201 aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc aataatacct
    49261 aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat
    49321 cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta
    49381 ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc
    49441 agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt
    49501 tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa
    49561 ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag
    49621 tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct
    49681 ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc
    49741 aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt
    49801 tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg
    49861 cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg
    49921 tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc
    49981 tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg
    50041 cagtcatgtt gatcatggta ggaagcattc cttttatcgg aatgatcatt ccgaatcttg
    50101 tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta
    50161 gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac
    50221 cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg
    50281 gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta
    50341 tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca
    50401 ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca
    50461 atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag
    50521 tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt
    50581 cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta
    50641 ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca
    50701 gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat
    50761 ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt
    50821 cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat
    50881 cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac
    50941 acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg
    51001 gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc
    51061 ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt
    51121 ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc
    51181 gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt
    51241 atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag
    51301 atgtacagtt aacgattcct gaaaagaaat tgactgcttt tatcggtcca aatggtgctg
    51361 gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct
    51421 atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga
    51481 tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg
    51541 gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat
    51601 cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg
    51661 gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac
    51721 tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa
    51781 aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg
    51841 ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa
    51901 cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga
    51961 tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa
    52021 gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt
    52081 ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact
    52141 acattgacga taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa
    52201 gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag
    52261 gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa
    52321 tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta
    52381 atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc
    52441 attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct
    52501 ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt
    52561 gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt
    52621 gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat
    52681 ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt
    52741 tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa
    52801 gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg
    52861 aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg
    52921 ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat
    52981 aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat
    53041 agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt
    53101 ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat
    53161 catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca
    53221 tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa
    53281 atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc
    53341 tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
    53401 ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt
    53461 ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc cagcgttctt
    53521 attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca atcagctgac
    53581 cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt gtctcagcaa
    53641 gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc ttactccacc
    53701 gttttaataa ttgatagaaa taactctctt ctgctttgat tttatactcg cttaatatca
    53761 atccaaaact attcgttttt tttgaagcta attttttata aatatccaat gctttggttt
    53821 tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata ttttgtgttt
    53881 cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca aagaagtctt
    53941 ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa aaagtaattc
    54001 tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa
    54061 ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga
    54121 acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact
    54181 tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa
    54241 agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca
    54301 agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg
    54361 tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat ttcgattgat
    54421 cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc
    54481 agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc catccacact
    54541 tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg
    54601 caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag
    54661 cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc tttggcaagt
    54721 tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga
    54781 ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt
    54841 cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc
    54901 acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa
    54961 tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa
    55021 gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc
    55081 agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt
    55141 gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta
    55201 tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga
    55261 ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa
    55321 atccatcggt cacacgattt acagctgcag accttttggt tattattatg aatggcagct
    55381 gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta tgaaattacg
    55441 tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac atttgcaaga
    55501 ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa
    55561 gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat
    55621 gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc
    55681 gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa aaaaaatata
    55741 atatacattt tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcaca
    55801 ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag ctgtatttct
    55861 agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct gataatcatt
    55921 tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt cataagtatc
    55981 gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt aaataaagaa
    56041 agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat gttattcagc
    56101 atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga atataacaca
    56161 gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt tgatccagag
    56221 aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg aacaggaggg
    56281 gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat ttctactgcg
    56341 gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa accgacatat
    56401 gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt ctctcaacca
    56461 gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa atttacgaaa
    56521 gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa ttctgaatta
    56581 acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg aacaggtgtg
    56641 gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt
    56701 tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt agtttggaca
    56761 ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata aatacgtagt
    56821 catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg
    56881 atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt gaaaaagagg
    56941 aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat catagggtac
    57001 tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag caaactcact
    57061 tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga ggatttaagt
    57121 atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga caaacaagga
    57181 ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat tatttctatc
    57241 gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa tgggaagtta
    57301 ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa taagaagatt
    57361 agtttactct cggattttac atttaaatct tatttatact taggaaatca acgagataga
    57421 gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag taatcccagt
    57481 caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc tggccaacct
    57541 tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg ttcttctgat
    57601 cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc ttttgtcacg
    57661 cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt agctcctaca
    57721 tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga gcaaagaagt
    57781 cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga tctcattagg
    57841 cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac tttttttcaa
    57901 gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa
    57961 gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa gataactatc
    58021 agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca gactagtatc
    58081 gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa tcaaattgat
    58141 gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga ttattttatt
    58201 gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat tcctgaaaaa
    58261 gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca atcgaatgaa
    58321 ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta taaagataaa
    58381 gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg agagaagtat
    58441 aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc gaatacagaa
    58501 ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg caaactttat
    58561 ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag tagaccatta
    58621 agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc ttttggaagt
    58681 tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga gcttccagaa
    58741 gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc tgttacgatt
    58801 ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc tacagctcat
    58861 cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa
    58921 ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa tataacaaaa
    58981 ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca gagacaagat
    59041 tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga ccaaaacaac
    59101 aattatttat aacactccta gttattttag gggcactttt taacaatata tcagtgtcag
    59161 ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc gacaagagaa
    59221 aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa gcggaattga
    59281 gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca accaccaatt
    59341 caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc ctgatctttg
    59401 atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa agtgtccaaa
    59461 ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg gctggtggga
    59521 tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat caaagcttat
    59581 cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca ctgaacgaag
    59641 ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca agaaatgtca
    59701 tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa
    59761 aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga
    59821 ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag
    59881 gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga aaattttcga
    59941 gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa aaagatttta
    60001 caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt aaaaaagaca
    60061 ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta ttatgcatac
    60121 ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat aacaaatagt
    60181 aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa tataccattt
    60241 tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt gatcgcgaga
    60301 aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg tatattatac
    60361 aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat ccccagctag
    60421 ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg aattatgagt
    60481 tttttttaag caaagaagat cacagaagtt attcattact taaatattta gaagcaagct
    60541 caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg acatttctct
    60601 tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta gagaaaaatt
    60661 tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa tgttctagca
    60721 aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta ttggctttct
    60781 ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg agttattctg
    60841 tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa attttttttg
    60901 aaaagagaaa gttg
//
LOCUS       DAFELO010000007        59629 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFELO010000007
VERSION     DAFELO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.007"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(456..1766)
                     /locus_tag="LP452_000538"
     CDS             complement(456..1766)
                     /locus_tag="LP452_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL2013294.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3196)
                     /locus_tag="LP452_000539"
     CDS             complement(1778..3196)
                     /locus_tag="LP452_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2013295.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3436..5004)
                     /locus_tag="LP452_000540"
     CDS             complement(3436..5004)
                     /locus_tag="LP452_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL2013296.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5008..5418)
                     /locus_tag="LP452_000541"
     CDS             complement(5008..5418)
                     /locus_tag="LP452_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL2013297.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5415..6338)
                     /locus_tag="LP452_000542"
     CDS             complement(5415..6338)
                     /locus_tag="LP452_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL2013298.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6335..7213)
                     /locus_tag="LP452_000543"
     CDS             complement(6335..7213)
                     /locus_tag="LP452_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL2013299.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7200..8537)
                     /locus_tag="LP452_000544"
     CDS             complement(7200..8537)
                     /locus_tag="LP452_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL2013300.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8540..9643)
                     /locus_tag="LP452_000545"
     CDS             complement(8540..9643)
                     /locus_tag="LP452_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2013301.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9717..11225)
                     /locus_tag="LP452_000546"
     CDS             complement(9717..11225)
                     /locus_tag="LP452_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2013302.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11191..11883)
                     /locus_tag="LP452_000547"
     CDS             complement(11191..11883)
                     /locus_tag="LP452_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2013303.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11905..12879)
                     /locus_tag="LP452_000548"
     CDS             complement(11905..12879)
                     /locus_tag="LP452_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013304.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13179..14051
                     /locus_tag="LP452_000549"
     CDS             13179..14051
                     /locus_tag="LP452_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2013305.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14509..16707)
                     /locus_tag="LP452_000550"
     CDS             complement(14509..16707)
                     /locus_tag="LP452_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013306.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16939..17931
                     /locus_tag="LP452_000551"
     CDS             16939..17931
                     /locus_tag="LP452_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2013307.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17991..19412
                     /locus_tag="LP452_000552"
     CDS             17991..19412
                     /locus_tag="LP452_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL2013308.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19405..20904
                     /locus_tag="LP452_000553"
     CDS             19405..20904
                     /locus_tag="LP452_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL2013309.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20943..22337
                     /gene="uxaC"
                     /locus_tag="LP452_000554"
     CDS             20943..22337
                     /gene="uxaC"
                     /locus_tag="LP452_000554"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL2013310.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22496..23179
                     /locus_tag="LP452_000555"
     CDS             22496..23179
                     /locus_tag="LP452_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL2013311.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23181..24017
                     /locus_tag="LP452_000556"
     CDS             23181..24017
                     /locus_tag="LP452_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2013312.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24028..25782
                     /locus_tag="LP452_000557"
     CDS             24028..25782
                     /locus_tag="LP452_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013313.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26058..26399)
                     /locus_tag="LP452_000558"
     CDS             complement(26058..26399)
                     /locus_tag="LP452_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2013314.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26467..28638)
                     /locus_tag="LP452_000559"
     CDS             complement(26467..28638)
                     /locus_tag="LP452_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL2013315.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVVYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28807..29706
                     /locus_tag="LP452_000560"
     CDS             28807..29706
                     /locus_tag="LP452_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2013316.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29777..30634)
                     /locus_tag="LP452_000561"
     CDS             complement(29777..30634)
                     /locus_tag="LP452_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2013317.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30619..33321)
                     /locus_tag="LP452_000562"
     CDS             complement(30619..33321)
                     /locus_tag="LP452_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL2013318.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33334..34623)
                     /locus_tag="LP452_000563"
     CDS             complement(33334..34623)
                     /locus_tag="LP452_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL2013319.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34773..35819
                     /locus_tag="LP452_000564"
     CDS             34773..35819
                     /locus_tag="LP452_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2013320.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35821..37974
                     /locus_tag="LP452_000565"
     CDS             35821..37974
                     /locus_tag="LP452_000565"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL2013321.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38447..39898)
                     /locus_tag="LP452_000566"
     CDS             complement(38447..39898)
                     /locus_tag="LP452_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2013322.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39895..41619)
                     /locus_tag="LP452_000567"
     CDS             complement(39895..41619)
                     /locus_tag="LP452_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2013323.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41612..42226)
                     /locus_tag="LP452_000568"
     CDS             complement(41612..42226)
                     /locus_tag="LP452_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2013324.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42571..44028)
                     /locus_tag="LP452_000569"
     CDS             complement(42571..44028)
                     /locus_tag="LP452_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2013325.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44069..44989)
                     /locus_tag="LP452_000570"
     CDS             complement(44069..44989)
                     /locus_tag="LP452_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2013326.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(45001..45948)
                     /locus_tag="LP452_000571"
     CDS             complement(45001..45948)
                     /locus_tag="LP452_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013327.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46427..47146)
                     /locus_tag="LP452_000572"
     CDS             complement(46427..47146)
                     /locus_tag="LP452_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL2013328.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47195..48841)
                     /locus_tag="LP452_000573"
     CDS             complement(47195..48841)
                     /locus_tag="LP452_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2013329.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49020..49610
                     /locus_tag="LP452_000574"
     CDS             49020..49610
                     /locus_tag="LP452_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2013330.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49702..51207
                     /locus_tag="LP452_000575"
     CDS             49702..51207
                     /locus_tag="LP452_000575"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL2013331.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51291..52643)
                     /gene="celB"
                     /locus_tag="LP452_000576"
     CDS             complement(51291..52643)
                     /gene="celB"
                     /locus_tag="LP452_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL2013332.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52643..53161)
                     /locus_tag="LP452_000577"
     CDS             complement(52643..53161)
                     /locus_tag="LP452_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013333.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53177..53503)
                     /locus_tag="LP452_000578"
     CDS             complement(53177..53503)
                     /locus_tag="LP452_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL2013334.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53516..55495)
                     /locus_tag="LP452_000579"
     CDS             complement(53516..55495)
                     /locus_tag="LP452_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2013335.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55516..55830)
                     /locus_tag="LP452_000580"
     CDS             complement(55516..55830)
                     /locus_tag="LP452_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL2013336.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55998..56291
                     /locus_tag="LP452_000581"
     CDS             55998..56291
                     /locus_tag="LP452_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL2013337.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56369..57481)
                     /locus_tag="LP452_000582"
     CDS             complement(56369..57481)
                     /locus_tag="LP452_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2013338.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57868..58047
                     /locus_tag="LP452_000583"
     CDS             57868..58047
                     /locus_tag="LP452_000583"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013339.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58241..58411
                     /locus_tag="LP452_000584"
     CDS             58241..58411
                     /locus_tag="LP452_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2013340.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58835..59575)
                     /locus_tag="LP452_000585"
     CDS             complement(58835..59575)
                     /locus_tag="LP452_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2013341.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17822 a        12189 c        10427 g        19191 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgcagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttctttttt tattgtattt gctgtaacag
     3181 cattttgtga aagcatttga attcctccta cctcttctag taaacgtttt caaaattata
     3241 actctttttt tcttgttttt atattcacaa tgttggcttt ttattgcatt atcttgacag
     3301 ataaaaaaca aaaaataatc taaaaacatt gatgcataag attgataact ttttttacat
     3361 aacaatatat acaaacaaaa aaattcacta actagtgaaa ataagttagt gaacctcatt
     3421 tattaaagaa ttaaattaca gatgatcttt tccatcatac ttgaaaaact caaaatcagc
     3481 ataacttcct ctttttttat ttagatcatg cactgctatc ccgataaaat ttccagtaaa
     3541 ccctcctgag agtatacaca tgtttttttg cggacctact ttcttccatt cttcagttga
     3601 tacgtctttc aaatgaaact gtgctgtctc tttctttaca gtgactttca aaaaagtctt
     3661 cttttctgtg agtggaatcg tccaatcttc tatttgtgct actccgtttt ctgattctat
     3721 catacgtagg cattttccct tgtcctcatt ccaagtaaca taacaataaa tataattgga
     3781 ttcatttaag tacagtacca gtccagccat ctgattaaaa ttattaggca tgtattcaaa
     3841 tgctgtctcc gcatcaaaag taaaatccgt ttgccgaata gctaacaaat gatgttcgaa
     3901 ggtagattga agagattcac cggaaatcag acgtaaaaaa cctggtctac attttgtatc
     3961 acaccaatca gcatttggca aaatgcgtct ggcattccag ttcttcttta gctctccttc
     4021 aaatgtatcc ataaactgat gatctttttc ttgttgtacc tcttcatttg tctcaatcat
     4081 tatttcttcc atcggagttg tctggctatc tttaagtctt aaccaatcgt cggacgtcca
     4141 atacacttct tgaatagcgg tttctcttcc caaaatagcc gcaccgttta atggtctggt
     4201 acaaagatat gtcatgtacc atcttcctga cggggtttgt actaagctcc catgtcctgt
     4261 acattgcaat gtagaagcag gtttgtcact agctgtcagc ataggaaaac caggatcaag
     4321 ctcgtaagga ccagtaatat tctttgctcg gcaaactgta actgaatgtc ctcttcctgt
     4381 accgccttct gccgtcatca aataataata cccgttacga aaatacaaat gtggtgcctc
     4441 cgttttcgct aattctgtac ctgcaaatat tttatagatg ggaccatcaa gcttcttctt
     4501 ctctgcatca tattcttgta atacgatacc gctggattta ttcggtgtgt ccagacgata
     4561 atcccatagt tcattgacaa gccatttttt tcccgtttca agatcatgga acaaagaagg
     4621 atcaaagccg ttactattta aataaattgg ttcggaccaa ggacctgtga tctcagatgc
     4681 agtgatcaaa taattgtgga catctttaaa cggacggcct gcacttttga catctgtgta
     4741 aacaagataa aacaatccat cagcataact gatctgtggc gcccatatac ttccatttgt
     4801 aggatttcct tgcaaagata cttgagtggt caaaatatcc gttgtatgtg accagttgac
     4861 taaatttttc gactcatata ctcggattcc tggaagccat tcaaaagagg agacaataat
     4921 gtaataccgc tctttcactc ttaatatatt aggatctgga ttgaatcctc ttaaaatagg
     4981 atttttcact ttgatagtct gcatgattca ggctcctttt ctcattctta ctcgatataa
     5041 actgaatgcc ccagctgcga aataaaaaaa tgcaaaagga aacgcaataa actgagtgcc
     5101 aatcaacaat atgaatcctg ttataaaata caaaccagtg gcagttgtta agtattttcc
     5161 tttagcagtc aaaaacaatg ctactgctaa aaaagcaagt gaaataaaca acacaacggc
     5221 aaacagacca gtcatgtcct gtaataatcc aaatgtattt tctaaagaca aagttgaatg
     5281 atctacagca agcgactgat aacttttttt aaactctgac gcagacatat tacttaaagt
     5341 aactgctaac ccgccaaaga aaatcaaaga tagactgcca ccgaccattc ctagcaatag
     5401 ttcccatttt cttttcattt ctgaaccccc tgcttttcta aatgtatcca ttcaggtctt
     5461 ttgatcgatg tttcataatt gttattacgt tcagagaatt tgacagtctg ttcatttttc
     5521 ttgtcatccc aattatccca accagttggg tgaacatgcc cacctatctt acacgcttca
     5581 aataagacat ttgcatacgg acgccacggt cttcctaaat agacgtttct agtatcaggt
     5641 tctgcagtca aggtacagct ttcaaaacga taccctgttt tttgattttt cggcgtagat
     5701 gctgctgtga tgtatccagg atttttactg gctaagctat ggatatgaca ctgatgaaaa
     5761 acggcatctg ccccaccaaa tatgaaatcc accgttcctt caatccagca atattcatag
     5821 gtctgacgac aatactcagg attggcagaa gccggtgtac aaaaaggagt accatctttt
     5881 tgcaaatcag gcaacggacc cgtgcataag gtatcctgat aaccagatag tcgacagttt
     5941 tttaaagata cgcgatctcc tgaattgaat aaagcaacag cctgtcctac cttttctcct
     6001 ggacctgctg tattttcgat gtgaagattt tctaatacga cattcgatcc ttctaagaaa
     6061 aaagtagcac ttcggaaagt ccctaactgg ctaccgtcaa gatactcctg ttttgcaaaa
     6121 cgattaccgg agataactac tgttcctgct cccacaagtt taaaattgga taatctgctt
     6181 tctatatatt catcatatct tccttctttt actatcaaga cctttggatg gctatccggc
     6241 aggcctgcca aataatcgat tccttcttgg atagtttcaa aatccgtcgg ggtatctata
     6301 gcattctttc caattatcag tttgtctttc atcctcataa atgctccctt atgaattgtt
     6361 tccaaatatt tctcatttct tgtgtttcca tatgaccgcc tgttaaagaa aaagaagaaa
     6421 agttttctgg actacccatt gattgatact ttttgtccag cccttttctc agcttcagta
     6481 cgccttcgtc caagcacatc gtatcgtttt ttccaactaa gctaagacga gggcgcggag
     6541 cgattttttc ttggattgct aacgttgtat aagctgtcaa tagatttggc acatagtaat
     6601 aaaacccatg atgatctagg cggcgatgta gcaaaagtat ttcgatgtcc acctgtgccg
     6661 ctagatcaat acaaactttg acacgagaat ccaatgctgt cacccaccag cttaataagc
     6721 ctcccataga aagaccaatc gttgcgagcc gttgagtatc tgtgtcttcc cttgtctcca
     6781 aataatcgat caagctgatc acatcataaa tacgcattcc ccataacgtt tttcctgtta
     6841 ataaaaattg tttgaacaac tcgctttcag aaatacctcc gcgttcttca aatccccaag
     6901 catcaatcga ccaaacagca tatcccattc ctgtcagtac ttctgcaaaa gatggagaag
     6961 ctaagtaagg agcaccatgg atcaattcac ttttcccgac tgaaaaatct ccgccgtgag
     7021 aatgcaggta aaggaccggc ggaaacggtc cttctttttc tggataagca tagagggctg
     7081 gtactgtttc caattcattt aagtacaatt gatagtattc aagcataaaa ccgttttgtt
     7141 tttctcggta taatcgtttt ccactagggg tatgtctgct agatggagca cctaaaaaat
     7201 cattcaactg cataagcaaa gtttttacga acaaccagtt catccatatt ttctgattga
     7261 tttgattgga atttctcaat actgatttct ttagtatttt ctaatagaaa tgctggtccc
     7321 tcgtgatttt caatcgacat ctggttaaag gaaaccttac ccgcaaagcc aatataaaat
     7381 ccttgtctgc tcatttcctc tattcctgtc atcatagcag gaacaccggg aactgcatct
     7441 tgggccatcg agacatcgat attgtcaaat gtgatttctt gtatgaattg ttccgctaat
     7501 ccataaataa aacctgcaga agcatggaca ttccttgccg tgatattcga aaaatggatc
     7561 cttctgaatg ctggtgtccg ttcatctatc ggataagcct ttttttccca tacatacggt
     7621 tctttccctc gcggaccaca gaaataataa agattcagta taaatggaca catcacatta
     7681 tccatgacta tattggatac tcgaatatct tcaacgatcc cgcctcgacc tcgtctagat
     7741 ttcaatcgta ttcctcgatc agtttcttgg aagatacaat ttgaaatggt aatattacga
     7801 atactaccac tcatttcact tcctaatacg acaccgccat gtccatggac catcgtgcaa
     7861 tttgtgatcg tgatattttc gcaagcgatc ctttcgtacg tatcttcagt ccctgccttg
     7921 attgcaatac agtcatctcc cacgtcaata tggcaattac taatacgcac atttttgcat
     7981 gattctggat caattccgtc tgtatttggt gaatccgctg ggttcaagat cgtcaagtta
     8041 tcaaacgttg cattactgca aagaatcggg ttgatcgtcc aacttggtga ctggatcaat
     8101 ttaatatctt tcacagtaat tcgatgacag ttatggaaac tcatcaattt aggacgagga
     8161 taagcgagat tatctggttc atttctaaaa gtatgccacc atttttttcc atttccatct
     8221 aatgtaccga aaccagtgac agagatgttc tctacatttt gagcatagat acaggaagca
     8281 taaacttttc gatggactcc ttcccaacgt gaatgaacaa caggataatc ttttggatcg
     8341 tcagagaatt tcagaaccgc tcctgcgctt aaatgaagtt cgacattgct ttttaaaaat
     8401 aaagctcctg ttagaaattc acctgctgga acaactacgg tcccgcctcc gtcacttgct
     8461 gcagcatcaa tggcttgttg tattgcttca gtattcaatt catcaattga tgcgccaaat
     8521 gtcaaaatat catacatatt tataatgcct ccacttcaat caatgcttgc aagaatgcac
     8581 caacgccttt ttgatcatta caaataattg gttcactgat gtagtaagca tatgaaccat
     8641 ctcgctgatc tgctccgcct aaccctgcta cttgacagtt tttattcaag ttcagccagc
     8701 cttcttttgt ttccagaaca aattcatcaa gtaatccttg atgtgatttc ttcacttgtg
     8761 gaagccattc ttctttgttc agcagtccca aacgtattcc tttagccatt gcgtaaacaa
     8821 acatactact gccggaagct tccagataat ttcctttccg ttttgtttct gttgttactt
     8881 gataccatgt cccgctgttt tcttcttgat atggaagtaa tgcagccagt gtcttttcta
     8941 gaatttcttt cagcaaagaa caatctgttt cttcatgaag taattcatag gtatctgctg
     9001 cagccatcaa ataccagcca atcgatcggc tccaaaaatg atgggataga cctgtttttg
     9061 gatcagccca tggttggacc ttcttttcat cccatgcatg aatcagcaaa tttgtttttg
     9121 gatcgaatag atgtttataa ctgatttcaa actgtctcaa aacatcttgg taatcttttt
     9181 cttcactaaa attctggata aattcagcat aaaaaggaga acccatatat aagccatcca
     9241 accaaatttg ataaggataa atttctttat gccaaaaagc tccttcggat gtgcgtggat
     9301 ggtttgccaa ctgctgggaa aggagttctg ctgctttttt atatttttct tcgcctgttt
     9361 ctttgtacaa taaaaagaaa agctttcctg tatttacatg gtcgatattg tattcttcaa
     9421 cgctgtagcc acggatcgtt ccatcttctt gcacgaaata gtcgatattg tcttttatga
     9481 aatcaaaata aattttttct tgggttcgtt cccaaagtaa ttggaaccct ttaagtacga
     9541 cgccatagtc ataagaccat tttcctttat accacttttc ttcatataag cgaggtgttc
     9601 tttccatgat actttttgct aatttctttg accagttttc cattatttgc tctcctttaa
     9661 aaattaaaat aggatttggg acaaatacca agaagtattt gtcccaatcg tctatattac
     9721 tgtgccgctg ctttgtatgc ttcctcgtac tcttgtgtga ttcgatcgcc tccttgtgct
     9781 ttccagtctt ctacagcttt tttgaatcca tcttcgtcaa gctgtcccat gatgtattgc
     9841 accgtagcat caatgacgat tttttcaatt tctgatccta cttcattgtt cgtcggtgaa
     9901 tccaatggca cagaaggatc cattacagca aattcttcat tttctttgat caattgatta
     9961 gagaattctt tttcagggtc tgtatctttg aaagagaacg tcacttcact aggacgagag
    10021 cttgataacg gttgcacctc ttgttgccaa agatccatat cgataaattc gatagaacca
    10081 tcatcattca attcataatg cgttccttcg ataccgccag tcatcaattt atacacatct
    10141 tcttctaaaa gatcatcaac aaattgaagt aatttcttca attgtgcttc gtcttttact
    10201 tgagatttgg gaaatgctag caagccgcca ataccatttc cttctgacca aacatgataa
    10261 ccgccgcctt caccatctga gatcttgttt acaggcacta attccatatc atcttgaata
    10321 ccttgaccca tcgtcttcag attcttgata tccaccattc cagtgtagat tccagttctt
    10381 ccttgtgcaa attgttcttg ctgatcagtc ttttgggtaa ctgcaaagtc ttgtgccaaa
    10441 tagccattct caaatagatc tcttgaaaaa ttcaaagctt tcatatactc ttctgtatca
    10501 aattctggag tgaatttgcc attatcgtct acagcccagc catttggtgc tccaaaataa
    10561 gaagtgaaaa gtttaaagct ggtataacga atatcactag gcgcacgatc gccaaaacca
    10621 actgtgtcgt ccacaccatt tccatcagga tcgttttctg taaattgttt agctacttca
    10681 taaagctcat ccatggtagt tggaacatct agtcctagat tatccaacca gtcttttcga
    10741 atgactaagc cagcacgagc gtagtctttt tgtatcggta cgccgtacaa ggcaccgtcg
    10801 atactcgcag cttcgattct atcttcagaa atttgactta aatttttata gtcttttaaa
    10861 tattctgata catcccagaa tacgcctgat tttaacgcat tgcggacaga agagtttttc
    10921 aacatcgcaa tttgaagtgt aacgatatct cctagttcat cagaagccaa tgcagttgtt
    10981 aatctttctt cttttgaagc atcaggaatc cagttgaatg tcagttccgt atcggttttt
    11041 tcttcgatca agttcacaat cgtgtctgta ggcggtgaag catgatgcaa aatatttaac
    11101 caagtgattt tagcttttcc atcttctgtc tctgaagaac tgttacttga actgctgccg
    11161 catgcggcaa gtgctagaat tcctgcaaaa ctaagaccta attttacgag cgttgctttt
    11221 ttcataaatt ttctccttca ctgttttaat aatatttttg ttttcttctg ttttttctgt
    11281 atatgagttc ttccattcca aatggcaaaa tatagaattt gctgtccacg tgactgcgga
    11341 aaagaagaga ctgccaccga taaaggttaa aacacctggc attgtttgga tgatcatcag
    11401 ataaatccct accatactta atatcaacac taatgagtaa tgaagatgtg agaatccgaa
    11461 taaaaatgcc attttcattt tgaaaaaata tcctttccaa tcataatgag ccatgattgg
    11521 gaaaatatag agtgctgaga gcacatatat gaacgcagca atatacaaag cgatcctaag
    11581 ataccaattg tgtatgaaag aaagatccac aagtaaaaca atcccgatgg cgccatatat
    11641 ccagctcatc agcaaggttt cacgatagtt ttctttaaaa tatgtgaagt acgttttcgt
    11701 caaaggaaac gtttcttttg agcgcagcca ttttctaatc actgcaacca tcgcatacga
    11761 tgcaggaccg aaagtaaaaa ctcccaaacc tgcgatgact aaaccgcccc acagaaaatt
    11821 caaataaacc aactgtaaaa tacgatttag ccttccgttt attttatcta atgactgcag
    11881 catatctttc cctctctttc tatcttagta aacgccgtct tctcctagct ttttggcaat
    11941 cttattagct aaaattacca taaacaatcc tacgacccct ttaaacaatc ccattgctgt
    12001 actgaaactt aactgtccat tcttcaatcc agctgtataa atataggtgt cgaaaatttc
    12061 tgcaacgctt cgattcaatg aattcaataa caagtacata tgctcgaacc ctaagtctaa
    12121 tgtctgaccg atttttagaa tcagcaaggt aatgattact ggacgaattg ccggcaatgt
    12181 aacatgccat gttttgcgta agcgtcctgc tccatccatt tctgcagctt catacaactg
    12241 tgtatcgact gcagttatcg ctgaaaggta gatgatcgta gaccagccca actctttcca
    12301 gatcacttgt ccgatatata ctgtgcgtgt ccattcaggg gatgttagaa aactgatttt
    12361 ttcaaatccc attgttgcaa ggaattcatt caatacccct ccgtcaacat tcaaaaatac
    12421 ataagtaatc gaaacaatga ttacccaaga catgaagtga gggatgtaaa tgatcgtttg
    12481 aatcccgttt ttcaatcgtt tgttcgttac ttcatttaat agtaaagaca aaatgattgg
    12541 catcggaaaa aacacgaaga tatttaatcc gaacaagatc aacgtatttc ttaataatgt
    12601 aaagaatgtc ggttctgtaa acaaacgaat gaaatgtttg aaaccgaccc aaggacttcc
    12661 cattattcct aaaaatggtt gataatcttg aaaagcaata acgagtcccc ccataggaag
    12721 atacctaaaa atcaagaaat aaaggactcc cggcagaatc atcacatata aaaatttatt
    12781 cgttttgatt cctgcaatta ttttttttcg tcttctgagc ttttcttctt ttttcgatag
    12841 cgtcagttga aatggttctt gtttctttac actttccata agcccctcct tagttttgtg
    12901 aatgtttgct gcttatgact tcatgataga taactatctg aattacgtat ataatcattt
    12961 ttaccgtaag taaaaacgct tacatagcaa tgatttttgt gctatacaaa acgataaagt
    13021 gtattttttc acaaataaac tacgcatagc aaaccttcta tttttttcta tctgacactt
    13081 agcataggaa atgattatat acgtaatctt tcaccgatga taaattgaac atatagaaac
    13141 agaaacattt aatttatttt gaggaggagc ctatctatat gaaaaagaaa gtctctatag
    13201 gagaaagaag ttttacactt ttcaacaata cgtttctaat tttgctagct ttgatctgta
    13261 tcgttccttt tttaaatatc atcgctacat cttttgcttc tacacaagaa gtcgtcgcga
    13321 aaaagtttat cctttttcct actacctttt ctttagacgc gtatcgctac attctttcta
    13381 cgccgaccat ttttagagca cttgctgttt cgattggtgt tacaggtgtc ggaacgatcg
    13441 tcagtatgtg tgcaacttca ctgatggcct acggtctttc cagaaagtat ttgtttggtc
    13501 gaggtttcgt caactttctt gttgtatttt ctatgttgtt cagcgggggg atgattccaa
    13561 cgttcttagt tgttcgttct ctagggctgg tcaattctta ctgggccatg atcctgcctg
    13621 tggctgttaa tgcaatgaat atgatcatta tgagaaactt tttccaagcc ttgccggata
    13681 gtttggaaga atcagcaaaa atggatggtt gcacagattt tggtgtattt ttcaaaatta
    13741 tgctgccatt agcgttaccg tctattgcta ctatctcgct tttttatgct gttacttact
    13801 ggaacactta tatgactgct attttgtata tcaacgattc atctaaatgg ccgatacaga
    13861 ttcttttacg ccaaatcgtc attgtatcaa gtgggatgca agcagaaagt agtgccgttg
    13921 atgtgattcc accagcacaa acagttaaaa tggctgttat cgttattgca acagtgccta
    13981 tgctgattgt ttatcctttt gttcagaaat attttgtcaa aggagcactc gtaggttctg
    14041 tcaaagggtg attatgtact gaacgcaaaa tggtagttgt gtcagaagtg gtcagcttca
    14101 agaaataaga aggcatttac gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt
    14161 tttcgaagaa tctactcctg gaacaccgtg tatcagaggt tgtgaaagag acgttcagcc
    14221 ttgagcgatg ataaaaactc tcacaaacat tgcgacgagt aacccacagg agcaataaga
    14281 agcgattttg aaaaatagct tcccatattt cacaaaatta tgccttattg tcgatagcct
    14341 gtctcttgaa cgccatttat tcgtaaaaaa gaactgtgac aagacttttg tcacagttct
    14401 ttttgcgtgc ttagccttga aatttaacta tccataaata caaatactag ggttattcta
    14461 acttctttta ttgcttgaat cagtccacaa gaaaaacagc ctcttgattc acaaatattt
    14521 ttttcgatat tctcccggcg taatcgattc cttcttttta aagaaacgaa taaaattttg
    14581 cggatttcga tattgcaatc tttctgcaat ctcttttacg gagaggttag tctctttcaa
    14641 ccattttttt gctacctcta aacgatgatt ttgaacaaaa tcaccaaatt tttctcctgt
    14701 ttccttttta aatacattac ttaaatagtt agggttataa tgcaacctat cagcgatcac
    14761 atctaaagac agatcacgat catattctgt gtagacgata tgcataatat tctccgacaa
    14821 atttttaaat tcatgatcag tgattgcttg agagcattct gtgattggca gaactaattg
    14881 ttctaaaaca agtttttcta gttctttggg atgataattg ttcagtgctt ttacataaat
    14941 ttgtttcatg ttatcaaagt tttttgaatc tactcccaat aactgtccta attgaacgag
    15001 ttcattgatc aagcggaccg aaactacttc ccgactcaaa ggatttttat tttgtgcaaa
    15061 caatgcatca atcaattcat gtactaaatt cttcacttcc tgatttccag aacgaatggc
    15121 ttcaaacaat cgattttgtt cttctatcgg atatctaata agtgtttttt catgagcagc
    15181 aggaacgatt tcctgataaa aaataattga acttggacca gtatttacac gataatacag
    15241 ggcttctttt gcccgatcaa ccgattgctt gctttcattc agcgtttcga accggttact
    15301 gatgcctata ctgatagtta aattcaagta ttcttttaca cgtttttgaa tgagtcggca
    15361 atactccatg acttttttgc ttgcttctgc atcatcttta tccattcgat aaatagtggc
    15421 ttgcatttct tcattcaaaa cgatcggcag cattcgattt tcctttggca caatttccgt
    15481 gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc ccaccgtgac tatcttctaa
    15541 cagatcgatt tgaaccaaag ctgtataata gcagtcattt tgagaactat aaccaaattg
    15601 ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt ctaaataaac taagtacaaa
    15661 tagcgtttcc agctgtggct tttgggtaac taagtgtgca cttaacgctt ctttttctcc
    15721 tatgatccga ctgactgatt gagcgaccaa atctagttct ttcccactga atccttttga
    15781 tttaagattc agacgttcct gtatttgaga aatcggacgg gcaaatctgt ccgaaaataa
    15841 gtaggaaaga agaccaatga gtcctaccag tgttaaagac actgcaacta gcccgattct
    15901 cagattgcgg atcattgcgc caattgcact agagtcaact tctaatctgt aaatccaatt
    15961 attataatct gatttcagta ctatatacgt tttatctttt tctcttatca ctttcagctg
    16021 attgtaatct agatccacgt ccgttaatac agttggttgc tctttatttt ttactgtctg
    16081 agtagtaaaa aggttgccaa cttcgttttc aatagaaagt atgtcttctc cactttcacc
    16141 aacaacttgt tctattgtat gtttctctat cgtagcaatt cccaatgcat atttttcttt
    16201 ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg tttttctttt gccaaaataa
    16261 attatttttg ttatttatgt acttctgttg atacttttca atttcctctt cggtcagttg
    16321 actcaacgat ccattgatta ctccccattt accagccaaa ctgactaaat cataattatt
    16381 atcatccata acaatcgttt ctataaaatt tagttctttc ctgatttcag aataagtgga
    16441 gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag aaatttttgg tcgaaccata
    16501 cgtagaaaac gaatattcaa tcgttctaat tttccattca acgttagttt taacttgcct
    16561 gagcaaattc tttttgtctg ttaaataact cgattcgatc gattcatagg tattctggta
    16621 aatgaataaa ctaaatccta tgacaagtac ggctcctaat aaaaacatcg gtaaaaatat
    16681 tttataaaat actgtacttt tcttcatttt cttcctctcc tccttttaaa ataaaaggct
    16741 ttcatttttc tgtaaaattg atactcatct aattaaatga gcattccccg ctttcaagaa
    16801 tagcataatt aaaaataaaa atccaagtaa tttttaacgt gtgcaatctt ttcatatcaa
    16861 agatctattg acttatcgta gttatcgtaa tatgatgaat gcgttaacaa aaagtgaaaa
    16921 agtaaggaaa atgatggaat ggtaacgatc aaagatgtcg ctaaagcagc tggcgtttct
    16981 catacgacag tttctcgtgc tttgaataac agcagtttaa taaaaaatga aacaaaacaa
    17041 aaaatacgac aactagctaa ggaaatgaat tattcgccaa actatagtgc aaaaggttta
    17101 gtgaatcaaa aaaaatatct aatcggacta tttttctcaa gtttgcataa aggaacttca
    17161 tcaaatttcc tagcagattc cattgcaggc atccattcag tattagacac aaattacagt
    17221 ttatctgttg aaagtatcga tgttgttgat ttaacagaaa tcaacctgca acgctacgat
    17281 ggactgatcg ttatgagtca aagtgatgat gatcagcctt tcatcgacta tctcaaagaa
    17341 aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg atccagcggt tatcaacgtc
    17401 actgccgatg atgcggccgg tgttatgcag gcagtagatt acggtattgg atgcggacat
    17461 acgaagatcg cttatattgg cggaaagaaa aattttcgtt cgacaaaaga acgggaaaaa
    17521 ggggtattga aaagtctaaa aaaagcgagc atccctgtca atccagccta ttttttaaca
    17581 ggagattaca gcttagaaag cggcctccaa aacatggaat acctattatc actttctgac
    17641 ctgcctactc tggttttttg cggcaacgac gacatagcga ttggtgcatt gagagctgta
    17701 catttagctg gcctccgtgt tccccagaac atttctctga tcgggttcga tgattctcca
    17761 gtcgtctcct atctcaatcc gccgcttacc accgtacgta agcctttgaa agaaatgtgt
    17821 caagaaggaa caaagctttt actagatttg atcgaaggca ggacgatcga acaatctaaa
    17881 attgagctct ccaccttgtt gatccttcgt gagtcgatca attacttgta aactacaagt
    17941 aattttatat aaaaatgtta acgtgtgcat aacacagaaa ggaagtttat atgaaaaaac
    18001 tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa agttcttcag tttggtgaag
    18061 gcaattttat gcgtggattt attgattggc agattcagca gctgaataac caacaattgt
    18121 ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg tcttggaaat atgatgaacc
    18181 agcaagataa tttatatacg gtcattttac aaggactcag caatggcgaa atcgttgata
    18241 cttccgagat tatcaccgta gtagaacaaa tgatcaatcc ttatgatgaa tgggatgctt
    18301 atctatctct agcagaaaat gacgacttgg cctatatcgt ctccaataca acagaggcag
    18361 gaatccaatt cgtggagact gataatttaa cagatactcc cccacagagc ctcccgggca
    18421 aactgacagc ctttttatac cgacgtttca aattaaacaa agctggcttt accatcattc
    18481 cttgcgaact gattgacaga aacggcgaac gtctaaaaga aatcatttta cgctacgcag
    18541 aaaaatggga tttagaacct gcttttgttg agtggttaga aaaagaaaat gttttctgtt
    18601 gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga agatgcaggt gttttcaatc
    18661 aacggcataa ctatgaagat caattgatgg tcaaagcaga accattcatg ctttgggtga
    18721 tcgaagggcc taaagaattg gagaatcaac taccattcaa acaggctggc ttgaatgtca
    18781 tcatcacaga tgatatgact ccttatcgtg aacgtaaggt gcatttgctt aatggaccgc
    18841 atacagcgat ggtccctctt gctttgttgg cggaattgga aactgtagaa gaagtaatga
    18901 aagatccttt attctccgca tatgttgatc agttattcaa tctagaattg attcctatgc
    18961 tttctttacc taaagatgaa ttagccattt atgctgacca aatcaaagaa cgtttcttga
    19021 atccttttgc ccatcacaaa ttagaagcga tttcattaaa tagtgtttca aagttcagca
    19081 cccgtctgct tccagtgttc aagaagtata tcgaagaaca aaatcaggta ccaccgttaa
    19141 tcactgtttc actagctgcg ctacttctta tgtatcgagg cgatcaagta aagccacatg
    19201 atgatgaaaa gacgataagc gaatttactg atgcttggtc tgataatgga acagctattc
    19261 ctcgattatt gcaaaacgct gcgctttggg gagaagattt gtctcaaata ccgaatgtga
    19321 cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa aggtgtccgg tcagtattac
    19381 aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc aaccaacaat gaaattaaat
    19441 cgacgagaca gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt
    19501 gatcagcaaa cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt
    19561 gatttgaaag ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca
    19621 gacgtaactg ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa
    19681 ttggattatc gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca
    19741 tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta
    19801 cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa
    19861 catccagatc tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg
    19921 cagcttggaa aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc
    19981 aatgccggag gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc
    20041 aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt
    20101 tacgatgaga tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat
    20161 gaccagcgca tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct
    20221 gatggtctat cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt
    20281 gctcaaggag gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta
    20341 ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac
    20401 ttcaaacagt atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat
    20461 aaagcaggtg ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga
    20521 agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc agggcttagt
    20581 ttattgcaag ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc
    20641 cagttggtct tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc
    20701 aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca
    20761 ggcgtcttat tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc
    20821 gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt
    20881 tttaaaaacg gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat
    20941 ttatgttttt atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt
    21001 cctacgcaaa aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt
    21061 acgaaaacaa caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat
    21121 ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt
    21181 ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca
    21241 gttggtcaca tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa
    21301 atgcagagaa aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc
    21361 gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg
    21421 acttgcacta tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat
    21481 tccgtccaga taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac
    21541 tggaagaagc agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc
    21601 agcggataga atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct
    21661 tacgctataa agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta
    21721 acaaactatt atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga
    21781 cccacttcta tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact
    21841 gtaatacaca aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc
    21901 tttctttgac tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc
    21961 caaagacgat cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg
    22021 gctgtttcca aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg
    22081 atactcgagc tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga
    22141 attttgtcgg catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt
    22201 tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag
    22261 atgaacaact tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact
    22321 ttggcttttt tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa
    22381 ataacggaat ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt
    22441 cttctcttgc tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc
    22501 tactatccat attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga
    22561 gacaggttgg ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca
    22621 agcaaagaat ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc
    22681 ttcacagttt cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat
    22741 ccaagaagaa agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt
    22801 tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga
    22861 ttacttagaa aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg
    22921 tgcatttgct gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt
    22981 gttccagacc ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac
    23041 ttgtaaaaat tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga
    23101 taaagttgcc cgaataattg ttgaattgat aaaagaatcc cgtctgcctc ttgctaatta
    23161 tttacaaaaa ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag
    23221 atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc
    23281 aatttgcacc acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga
    23341 gtgctgccta ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat
    23401 gctatatcaa cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca
    23461 aacagcaaat tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta
    23521 gtgtctctgt cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa
    23581 attctatcat tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag
    23641 cagggatcaa tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag
    23701 cttcaccaaa tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg
    23761 ctgatcagga aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc
    23821 ttcatctcaa agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg
    23881 gctgtttaga tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg
    23941 ccactcttga agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta
    24001 tttaccaaga ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa
    24061 tatcatgcaa atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt
    24121 gccatgtcag gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct
    24181 attgccaatg taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat
    24241 ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat
    24301 gtaaaatacc atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc
    24361 aagaaaatcc aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag
    24421 tcaaagatca tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc
    24481 ggattaaata tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct
    24541 gttgttttca tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc
    24601 cgtaaaagga tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca
    24661 aaagtgatcg aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac
    24721 gaaacaactc gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat
    24781 atcctgcaat ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt
    24841 tgtttgattt tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg
    24901 atgtatcaga cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt
    24961 cctacgattt caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc
    25021 gatgtagaaa cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt
    25081 gaggatgttt cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta
    25141 gaggtgaaag ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca
    25201 ctccttaact tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat
    25261 cagcctttgg atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa
    25321 accactttgt tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta
    25381 tcaaaagaac gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag
    25441 cttcctgatg gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa
    25501 aagcaacgta tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat
    25561 gaagcaacct cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac
    25621 ctgacagaaa cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca
    25681 gataaaatcg ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg
    25741 ttagctcaaa aaggctattt ctaccgcttg aaaaatgaat gacaaaaaat aggagctatg
    25801 acaagacttt tgtcgtagct cctatttctg aataaacggt gttctcctgc caactcctcg
    25861 gtcacaagtc acaatagtca tcaaacatga gaagctgttt tttgaaattg ttccttgtaa
    25921 ctcagagtta aacggtaggc tcacgacctc ttggtaaatg gtgttcaaga gacagcctat
    25981 cggcaataag aagcaatttt gtcatcgctc aaggccgaac gtctctttca caccctctgt
    26041 tgtttcataa aatagtttta ttcgctgtaa aggtcgcgaa ctggtgtcat gatgatacgg
    26101 ttcaagtcat tgatgattgt actgaagcct tgctcttttt gcatcaagtc attgattact
    26161 tcttctgatt ggattttcac agccatttct tgtgctttca ttgcatcttc gtcgctgaac
    26221 tcttgtccac tcatttgttt ttcttgtaat tcgatttgca tcgcttggaa gtctttgaac
    26281 agtttgtgtg cttcttcgtt tgcttgtact ttctcgtacg cttctttcaa cgctttgaat
    26341 tctggtaatt cacggatctc acgttcgatt tgattcgcgc tatcgtagat gtttgacatg
    26401 ttatgccctc ttttctttta gtagttactc tctattgtaa catggggtta ttcgttttca
    26461 aaggggctac tctccttgat atttccgcca gaggtcttta gccgcttcac caattccctg
    26521 aacgccttct ttcaccatat cgccgaagtt tttcagccct tcttttgctt gttcaccata
    26581 ttgattcaag ttatcttgcc aatttccaga ctctccatta gaatttcctg tgtttccaac
    26641 ttgatctgca gctaccactt gtcctcctgt ggcataggca tctgctacac taaatcctgt
    26701 ttttgaagta tacggaagga tgccttctgc cgctgatttg aagactttcc cgacaacatt
    26761 tccacttgtc ccttccaaat agtgcagctt gctggattct tcgtaaccca tccatgtcga
    26821 aatcactacg tctggcgtgt aaccgatcat ccattggtca tttgcttttg tggcatcgaa
    26881 attagtttct gtggctcctg ttttccctgc tatcgtataa cctgcaggat tcgcagcagc
    26941 cgctgttcca tttgaaaatg taccaagaag catgctggtc atttcatcgg ctgtttcctt
    27001 tgatatgacc tgttttggtt ttttgtttgt gttatcgacg atcaccgctc cagtcgaatc
    27061 aacgattttc gtgataagat gcggcgtata catctgacca tcattggcaa atgcactgta
    27121 agcacccgcc atgatcaatg gagatactcc tttttccagt ccaccaaggg ctaagccata
    27181 atacttatca gattttgcta aaggcaggcc gaattcttct gctttatcat atcctttttg
    27241 taaaccgatc tcatgcaaaa gccatacagt aggcaaattc aaactttgtg caacagcttg
    27301 atacatcggt acttctcctt ggtatgtccc gtcaaaatta tgcgcatcgt aataactttg
    27361 cggttcgtct ttcaagatac tggaaggctt gtatccagct tccagtgctg gtgtatatac
    27421 gcttaatggt ttgatcgttg atcctggtga acgacgcatc tgtgtagcaa agctgaatcc
    27481 tctaaagaca tgatctcctc ttcgtcctac tagtgcttgt acaccacctg tattcggatc
    27541 taaagctact gaagcagatt ggaccatggc accatctgaa gcatttgctg ggaataatgc
    27601 actgttttga taagtagctt ctaattgctt ttgataatcc tgatctaaag ctgtatagat
    27661 tttatagcct ttattcatga catcttcttc atcaagacca tactcgttaa ctgcttcatc
    27721 gatcactgca tcaaaataat aaggataacg ataaccagaa tctgaattat cgtatgtgtc
    27781 attaagatac gcgctgatat ctgtctggct ttctgcttgt tcttgttctt ttgtgatttt
    27841 tccgttttct tccattacac ttagtactgt gtttttccga tcattcgcat tttttgggtg
    27901 atcgatcgga ttataaatac caggaccttt cagcattcct gccaaagtag cagcctcaga
    27961 cagcgtaaca ttcgttgcat ctactccaaa atactttctg gaagcgtctt ggacacccca
    28021 taccccgttg ccaaaatagg cattgttaag atacatggtc aagatttctt ctttgctgta
    28081 cttcttttcg atctcgattg ccaaaaataa ttccttcgct tttcgatcaa acgtttggtc
    28141 taatgtcaga tacgcatttt tggcaagctg ctgagtgatc gtacttcctc cgccccctga
    28201 tgttccgcca gacaagacca ttcggactgc tgcacgggca atccctttga tatcaaaccc
    28261 gtgatgctcg taaaaattcc tatcttctgt cgagattact gcatcttgga tatagggaga
    28321 aatcgcgttt aattctacat atgttccttt ttgggcgtac agtgtgccag cttcttcgtt
    28381 tttcttatca taaacgactg tcgattggct taatcctgat tttaacgttt cgacatttgc
    28441 ttgttttgct aaaataaaga ggtagatact tgtcactaag acacagatca aaccaatcag
    28501 caaaatcaat ttgttcacat gatatttttt ccagatgcgc tttcgccatc gatggaattg
    28561 tccgagatat ggtctgatcc aacgccaaaa actgatcaaa gcagctttta tcttttctaa
    28621 aatgctttga aaatccattt gcttcttctc ctgtctattt gattcaatcg tcttatttta
    28681 ccatagtgaa agagtgtttt cttcctgctt aagttgtagt tcaatatgtt tttttacgtt
    28741 agtgcaagta gagtaacata agaatgaaca gaaaaatgta agttttttct taaatatgtt
    28801 ttaaaaatgg tacaattagt ctgcgattgc actgaaagga gtacacttgt gaaaatcacg
    28861 atcactttac ctatgaacca tcctgaaaca acaattcgcg acttgcttga aaaggaatgg
    28921 ttagttccta gaaaggttcg acactttttg cgtatacgta aaaacgtttg gatcaatcaa
    28981 caacctgctt tgttccatca tgaagtaaag ggcaatgacc agatcacatt gttattcgaa
    29041 gaaacagatt ataattatca agaagttcaa ttaggagatg cgaagcacat ccgtgtttta
    29101 tatgaagatg agcatttgat tatagtggat aagcctgcag gaatgaagac tcatccaaat
    29161 gaaccgacag aaaacagtac cttactcaat catttagctg cttatctcga cactaaacag
    29221 caacgacctt acgttgtcca ccgcttggac aaagaaacaa gcggtgccgt tttatttgca
    29281 aaaaatccat ttgtcttgcc gatacttgga agaatgttgg aacaaaaaat gatctatcgg
    29341 agatatcaag ccatcgtatg gggagaaatt gccaaagata tgaccatcac caataaaatt
    29401 ggacgagatc gccatgatcg gagaaaaagg gtcgtagatg ataaaaatgg tcagagcgcc
    29461 ttgacacatg tcactgtcaa caaagtactt tctgaacaaa cacaagtcta ttgtgttttg
    29521 gaaaccggac gtacacacca gatcagagtt catttagctc ataagggaca tccgatcgtt
    29581 ggagaccctc tctaccaaaa tagaacagct gaacgactga tgctccatgc attagaactg
    29641 cacatgatcc atccatttac tcaagaaaaa atagtcggta aagctttacc tggattatgg
    29701 gaatagaaat aaaaaagaac agagtcttat cagcagataa tgataagact ctgttctttt
    29761 tgtgtaattt gtctgcttac ttttgatgtt cgtgttctgc taacagcaaa ctaccgataa
    29821 tcccactttc attcggcagt ccccaacgaa cgatatattc acttacttga ggtgtttcca
    29881 tataaccacc catcaattca acaaattgct gacggatttt ttgaaggaga tgatcctgat
    29941 tcattacccc accgcctaaa ataatctttt caggtgctaa cgtcaacgta tagttgatca
    30001 gtgcttgagc aatataataa gcctgaatag cccaaacagg atgatcttgc ggcagatctt
    30061 gccctttgat ccctgtgcgt gcctcaatag acggtcctgc tgctaatcct tctaaacaat
    30121 ctttatgata aggacaagta ccttcataat ggtcatctgg atggcgtttc acccaaatat
    30181 gtcccatttc aggatgtgcg atccctgaaa aaatttcttc attgatcacg acacctgcac
    30241 cgattcctgt tcccacggta aggtaaatac aactattttt tccttgtgct gctccttgct
    30301 tcaattcccc atatgcagca gcattgacat ccgttgtcca agcagcagga atattgtagc
    30361 gttccttgat tgtgccaaga aaatcgaatt gtgcccaccc ttttttggga gttgccaaaa
    30421 cgtatccgta attatctttt gttttgtcga taccaatcgg gccgaatgag ccaatcccga
    30481 ttcccttcaa ttcataacga tcaaaaaatt caaatacctg atccaatgtt tcttctggtg
    30541 ttgtggtagg gatacttaat ttttctaaaa tttctccacc atctgaaacc gcacacacaa
    30601 attttgttcc gcctgcttct attccgccat acattcgttt tcctcctcga atccgattgt
    30661 ccgaatctca taaggttgga ttattgggac aaccgcttgc ttcgttggtt cttcaagcag
    30721 attcaatatg acagtttttc cattgtcttt agttatttcc aatttttcta aatgactaga
    30781 catattgaaa cctcttacta ctactttatc actaaacttg ctgcgtttca aagctgtaat
    30841 tgcaaacgtt tcacttttta tcgttaaata ttgttgtttt gaaatcaatg ttccttcatg
    30901 atgtttgatt tgttgagtgg aaaaaggaat ctgtgctgcg tatgcatgca aataagtaga
    30961 gaatttttct tctggtccat gtagttcaat gctgtaattg aaccgatgct cacctagaca
    31021 ctgtgcttct ggtgttggaa aatatcccca atcgcctagt tctcccacac tgcgaagtag
    31081 cgtcactgcg atttgtccgt cttctgttac ttcatattca ttcaaaccaa agtttccgac
    31141 tgttacaccg tactcttcat catgaatatt gacaaatgct tgctgatgtt gcggattcgt
    31201 ggggttttcc caacttttag acacatggtt tgggcgcttc accacttcaa aaatactatc
    31261 tgcttcgtga tgttccacat gtaactttgt aggaaacaag acacgtaagc gatgatcttt
    31321 catctgattg tcgagagtcg tttcaaagtc gattttttta ctatcttttc tgattgtcat
    31381 gattgttttt atttgaagga ttcgtttttc tttggatcgt tctgcttttc gataacgaaa
    31441 ttccacaacc atctgctgtt ctttttctaa acgctcgtct gcagaaacag ggatttccat
    31501 ttcctgtaga atacttattt ttgctatttc cggtgtatct actaatactt ttacttcaga
    31561 gttttctttg ttactggata ggattggctg atcacagaaa ggttgtttaa agatatactc
    31621 atttcctata tcgccgatat cttcaaaaat atgcagatta ttcaactttc gtttgttttt
    31681 gcgatcttcc attgtaatgg taccattttt ttcaatcgtc aaatgtagag ggccgttttc
    31741 gatgattcgt ccactctgat gaatcattgt ttccttttct tctgctttcg cttccccttc
    31801 tacaagcgcg aatgtttccc aagaaaatgc tggcatgttt tctaatggta aagtcacttt
    31861 gatcattctt gccatatatg gcacacggaa acggtctttc ggcaaatcat aaccaaattg
    31921 gacagtttct tcagataaaa tagctggtat agtggcccca ctttttgttt ctacatgata
    31981 cttacgttta ggcaaatttt ctaactcttg atatagttgt tcaggtattc cttcagcgaa
    32041 tctttttctt tctaattcga tggttatttc tgcttcacca gttttatcca ttcctgcagt
    32101 atttacaatg acaaatggaa agctgtcttt tgggaatccg gtcgtatcga ttgctgcggt
    32161 cagctgttct aaggaatctt gtgcaagata ctttccaact tcatctgctt tttcaaaacg
    32221 aggtatcatt tcccgatgga cagaatcgac tgaacaaccg caaatactgt cgtgaggatg
    32281 attttgcatt aatgtcttcc aagcataatc taattgatca tggggatagt ttccactaac
    32341 ttcataagcc atcgtcgcaa gtggttctgt aatattttct agctgtcgct ggaccttggt
    32401 attccattgt tttaagtaaa cccgagcaga tgctgtattg gctaaagtat accagccatc
    32461 cgtttcttga cttgtcaatt ctccttctac tgagcctaag tcttctggca catctttttg
    32521 aactgcttcc agatagtcag taaaattaga atgaataaac tcgtaatctg gaaacagttc
    32581 atttgcgaga tggattgctt ttgatatatc tttttgtacc ggttgatgat caacaccatt
    32641 catcatcaat aagtggttcg tagaggcata ttgttctgca tccgctaatt tttgcttcca
    32701 aaaggcaaga gctgcttctt tttctactgg aatctcattc ccgttgctat accaattggc
    32761 aaatagcaga ccaaagattg ctgtttgatc aggtcctttc caccacatct ctgagtattg
    32821 ggaagaatag ttttctgctt ccaaaacttg attatcaaag ccaattggtt ttacaccacg
    32881 gccaaacgct gctgctgaaa taccagcttg tttcattaat tgaggcgtct gtcccatatt
    32941 tccaaatgta tcgggaaaat agcctaacat gacaggggtt ccccatttac ggctttcttc
    33001 catgccaatc agcatatttc tcacgttcga ttccgagctg ataagaaaat catcttgcaa
    33061 gatataaaat ggtccaattc tcaattttcc ttcattgatt gcttgttgga ttgcctgtct
    33121 tttttctggc cttacttgaa gatagtcatc taaaataatc gtttgcccat ccaaatgaaa
    33181 actgttgaag gacggatcaa cttcaaatag ctctagtaga tcgtctatca attcaatcag
    33241 tcgcatgtga tgctgttcgt aagccatgta ccattcacga tcccagtggc tatgagaaat
    33301 gatatatact ttctttttca tttacgatac ctcctacttt tctactctaa tatcaaagta
    33361 atccatgact aattcgcaaa acatcatatt tgcccaagaa aaccattctc tcgtataatt
    33421 ttcaggattg ttcacatcga atccttcgtg cataaggtgt gttcctgcgt cagttgctac
    33481 aagtaaattc aatatccgct ctttttcaga tttgtctgac gttgtcattc cttccatagc
    33541 taatgcaatc ggccaaacgt atttttctgg cgtatgggag gatccaattc cttttgcgta
    33601 ctttccttca tagaaatatg gattttcttt actcaacaat gttcttcttg tttgcagata
    33661 atcttcctct tgttcagaac aatatcccaa gtaaggtgcg gcgatcagat tgggcacatt
    33721 actgtcatcc atgactgtgg cgtttcccat tccatctact tcatatgcat aaatcgtttg
    33781 ccctttttga ttaaccgttc gtccgtgatt ctgaatcccc tcttggatct ctttcttcaa
    33841 tttctgtgtt tgctgtacaa tttctttatc atcgagtatc gtttgaaaaa tttcttccac
    33901 ataacctaac acgaccaccg caaacatatt ggatggtacc agatatccat aacggcaagt
    33961 atcatcgcta ggacggaaac cagaccatgt cattcctgta aaagctgttt ttgcacctcg
    34021 cccgtcattg atcagcgtat cttcttttct tgtcgtgtct cgtacaaatg tataaggaga
    34081 gtgttcatga tcttgctctg ttttgaacac gtccaagatt tttttgatac cttcaataaa
    34141 agattgattg aattgatcag tttttcctgt atttttgtaa agaagatagg ccaactgtat
    34201 tgggtagcat aaagaatcaa tttcatattt tctttcccag atccaatcgt tcatttttgt
    34261 atgatctgtt tggtggccag caccgttagc ttcttcgttg aaggcattcg catatggatc
    34321 gatattgata taaaaaaatt gctttttgac taacccgctg atcatgtcag ctaattcctc
    34381 atcttctttt gcaatcacta aataaggacg aacttgtgca gtagagtctc ttaaccacat
    34441 cgctggaatg tctcccgtca ataaaaaggt ggttccgtca ttttgctttt tgactgttgt
    34501 caacagagta ttggcaaatg ctgctgtaaa attctttgcc cagtccgcat ggatatcccc
    34561 acatttttgt gtgatatctt ccataaattt ctgcaccgat tttggtatat ctttataagc
    34621 cactaataaa accttctttc tatttgatat atctttaaag attatacttc atacagccaa
    34681 ttatgtctac gctttcaaaa aatatctttt tatttacttt ttcatccatt atggtattat
    34741 ttgaatatga tatatcatta agaggtgttt atatgaatca accattgtac cagacaatat
    34801 ttaatgattt gcaaacggct attttaactg gtgaattacc tgtagacagc caactaccaa
    34861 ctgaaaaaga actttcacgt acctataaag taagcagaat cacttccaaa cgtgcattga
    34921 ctgaattaga gcaattagga cttatttatc gcgttcgcgg gaaaggcagt ttcgttaaat
    34981 ccgcttctcc ggaacagatt cttgcttctc ctacaaaaac acgacggatt ctgtttcttc
    35041 ttccatatgt cgctgatttg tctgttggtg attttagcaa gggattgaac cctgtgatgc
    35101 aagataaaca gattgatgtc atgatgacga cgcttgactt tctacaaaat aaaacagcaa
    35161 aagagctaat caatgaattt gacggattga tttattatgc tcttgataca gaacaacatc
    35221 ttgacttact ttttgagctt tcattgaaag aattccctgt cgtcatatta gataaaaaaa
    35281 tctatgagtt accttttcct accgttcttt ccgataattt ccaaggcggt gcgcttgctg
    35341 cctcttattt gatcgaacag ggacattcaa aaatcgctta tttgtttggt acgcagactc
    35401 atccacaatc cgttcgacag cgttaccttg gttaccttga agcattggac aaagctggtt
    35461 tgactttcca cacttcactg gatgacaaaa gcgcgacatt agatgaattg ctttcttata
    35521 tcactaagta tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct atccagacca
    35581 tgaatctctt gagacagcat ggttatcata tccctgaaga tttttcaatt atcggatttg
    35641 atgatatcca agcagcagct cttgttgacc cgccactgac aaccatcgca caagattttg
    35701 aacagttagg taagcttgcg ggagatgctt tggtcacttg gttagagaca aaagatcgac
    35761 catccgatag taaatttcca gtttcattca tcaaaagaca atcaacaaag gagaaataaa
    35821 atgaatatcg gtcaaataga tactagacat gcaacagcga atcagccaaa tttctctaat
    35881 gggaactgcc agccttatac tggcgttcct tttgggatga attattttgc accgcaaact
    35941 acggatcaaa acggcagctg gtggtttcat cctgatgacc gcgtttttca aggctaccgt
    36001 cttacccatc aaccaagtcc ttggatgggt gatttcagtc atatgctgtt tacgccgatc
    36061 aatggaaaac tacaggagaa cacccttttc catgctcaaa gttcttaccg cccagaagaa
    36121 agtatatttt gcccgactca tctgtcgatc tgtcaattac gttatggcat ctgttctaca
    36181 ttgataccta gcatgtatgg cggaatctta tctattgatt acagcaaaaa tgacagcggt
    36241 ttacttttat cctttccggg acgccatcag ctctttgtta tagatcctta cacagtggaa
    36301 ggaaaaatca cacattttgc tggttcagaa gatcctgatt tttcttttta ttttatattg
    36361 agatttgaat atcctttaac aattgatgag ttagctgttt ccaacaaaaa tagcgactcg
    36421 atcccgttgt attttgaaca gacaaaaaaa caaactatcc gatttgggac atcttttatc
    36481 agctcggaac aagcagctta taacctggaa ctagaacaaa accaaagcaa agagacttac
    36541 cttgctcaca gtcaagaaca atggcagtcc tattttgatc gtatacagct cacacaccat
    36601 gatcaacaac aattgcggac gttttatcat aatttatatc gggtgttttt atttccacaa
    36661 acattttatg aaattgatcc tagtgggcaa aaaatccact atgatacgac tagtcgatct
    36721 ataaaacctg gtgttctcta tacaaataat gggttttggg atacgtataa aacggtttat
    36781 cctctttatt ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg ttttttgaac
    36841 agctatagag aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg tggacttatg
    36901 cctgggacat tgatcgatgc ggtcattgca gatgctgctg taaaagatat ccgacgagac
    36961 ttgatgcctg aatttctaac ggcaatggaa aaaggtgcta cggttcaaag caaacatcca
    37021 aattatggac gccaaggaac agaagattac ttgacatatg gctatgttcc taatcactac
    37081 cacgaatcag tcaatcacac attagactat gcttatagcg atttttgtat tagccaagtt
    37141 gccaaggtac tagaagaaga ggaaatgacg aagaaatatc agcagcaatc attgaactat
    37201 caaaatatct ttgactatga gtcaggattt atgcgttcaa aagataaaga tggcaagttc
    37261 agagaaccat tttcagctaa tcgttgggga caagattatg cagaaggaag tgcttggcaa
    37321 agcagctttg ctgtttatca cgatttttca ggactgatcg gaaaatatgg tggcagtgag
    37381 agatataaag aaaaactcat tgagctgtgc aatcagcccc caacgtttga cccaggagga
    37441 tatggttttg agatccacga aatgagtgag atggccgcga tagaatttgg acaattagct
    37501 atttcaaacc agccaagttt tcactatcct tacttgttca gttatatagg acaacctgaa
    37561 atggcacagc cactgcttaa acagttgatg actcagacct tcgacgattc tccaatgggt
    37621 taccctggag atgaggacaa tggaagtatg gccgcttggt atatcttcaa tagtctggga
    37681 ttttatcctg tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt aattgacaag
    37741 gctgttttgc ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc taatcggccg
    37801 cagcaacaat ttatccataa aatcaaacgg atggacaaag aatatacgaa attatatttt
    37861 acccatcaag atttgctgaa aggcggcacg atctcttacc aattagggat catccctcac
    37921 cctaagaaat atcaagcaaa agatttgcct ttttccttat caaactataa ttaacaagag
    37981 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagatcctca
    38041 tatttttcgt ttggttggtc ttattaccgc agatgcaagc ttttgaacac cgtttattga
    38101 gaggttgtga aatcagcgtt ctgacctgag tattagagag acaaatggca aaatagttcc
    38161 tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg
    38221 gagaggtcgt gaaaaagctg tttagtttcg agtattaaga aaaattttgt gaaaatggct
    38281 ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga actccgttta
    38341 tcgagaggtc atgaaaaagc tgccttgcct caagtaataa gaacagccca aaattagatt
    38401 tctagacaat tttttataag ttatttcatt acattggctt tttcttttat gattctccac
    38461 tgttttctcg gtactcttta ggggatctgc cgacgatttt cttgaataat ttagagaaat
    38521 acgtggtgtt gttgtagcca atctcacttg ctatctcatt gatgttttga ttactgtgca
    38581 aaagcagttg tttcgctttt tccatacgta aatggttcaa ataagctgaa aaactttttt
    38641 tcgtttcttt tttgaacagc tgtcctagat acatgacatt taaatgcagg ttttctgaca
    38701 cttctttgag tgtaagtggt tcttgataat gctggtgcag gattacgatt actttttcca
    38761 cattgtcact gtactttttc tgtctctgat gctttttgat gaatgacaga agtagctgct
    38821 gaagttcttg caccgttttt gcctgatgga ttttttcgat tccttgtaaa tattcctcat
    38881 cttccagatc aatcaattcc cgttgaatct ccataaacag taaaaaactg gaatatcgta
    38941 tatcttccgg catcatggct gctaactgaa actgttcaaa aaaatcagtg atcatttttt
    39001 gagcgacttc caattgttta ttttgcagca cgcgtctgaa acttgaaaaa tcgatggttt
    39061 gatcttttgt ttctgcttgt actgcgtaaa aaacatgctc gtctttatct ccataaaatt
    39121 gatgcaactg tagattgtct ttcgcttgtt ggaatgtttc agggattttg tccaaagaat
    39181 ctgtctcttc cccgatactg atcagccatt cctgatcagg aaagttttct tgaacaaaac
    39241 aacagaagcc atcaacttca tctgcctcca acaacaacgt gatcaataca cggtctccgt
    39301 aatttcgttg atagtaaaag atttgttgcc gttcttgcaa ccatctattg ataagagtat
    39361 catgagttct tgccagttga atcaatacta tcctatgctg cttctcactt atttgtccta
    39421 ttatttcttc ttcacttatt tcatccaatt cttcattcaa ccattgactg aataacagtt
    39481 cttgatagat ttcttgttga tttttttgtt cattttgctg atctaaccgt gttttgattt
    39541 tcttgaggct ttcaaccaat tcgaacttat tcacgggttt catcaaataa ttcacggcgc
    39601 ctaattgcat acctcctttc aaataatcga attcttggta tcctgataaa atcataaatt
    39661 caaaattatg acgttctttt tgagccgctt caataaattc cagtccattt atttctggca
    39721 ttgtcacatc cgttactact aaatcaatct gtcgctgttc aagtacagct agtccttctt
    39781 tggcattctc tgctgttgct actatttgaa agcctaaact ttgccaatca attatctttt
    39841 ttaacccatt caaaatcatg tattcatcat ctaccaataa cacttgatac acgctcatcc
    39901 ctccttgaat gtaatagaaa cggtcagtcc acctgtctca ttattctcaa gcgacatata
    39961 ataactgctg ccgaagctgg cacgtaaacg ttcattcacg ttttgcagac cgatagaacc
    40021 gtgaagttcc actttaggat gagacagttt ttcttcaatc tgttttagcc gtttttcagt
    40081 catccctttt ccattatctt tgatgatgat ccttacccgt ttgccttcct gcaagacttt
    40141 gacacttagc gcattatcga atcgagtaaa atcaataccg tgtttaaaat aattttcaac
    40201 taatggctgg atcacaaatt taggtacttc gatcttttcc aaatcaggat cgatcataaa
    40261 atgataggct actcgattag gataacgcat ttgatacaaa tagacatatt tttcgcaaaa
    40321 gtccagttct tcttttaaag tgatcgtttt ctcttgattc gtattgtttc gaagcaaagc
    40381 ggaaaacgca tacacaacat ctgctaattc ttcacttcct tcgctgattg catacattcg
    40441 gatatattct aatgtattat ataaaaagtg tggattgatc tgtgcttgaa gcgcccgcat
    40501 atgtgcgtct tgctgtttga tttctaattt gtagatgtct tctacaaact gattgatgct
    40561 atccaacatt tcgttgatac cgattgaaag ttccttcagt tcatattccg tgtttgttat
    40621 gtcgatccgc gtctctaaat tccctgctgc tgtttgatcc atggcctgca tgacgataga
    40681 tacttgctgt gaatatcgtt tgaatgtttt ataaagaaag ataagcagta tattgtctaa
    40741 aataatgata ccaatgatca gcggggcaag attttttccc gtttcgattt ttacactttt
    40801 tttatctagt acagctaata tacgataatc atcagacagt tcttggtgtt gaacaaaatt
    40861 gtttttttcc agatcgtcaa tcggcaattg tgaatttttc ttcagactct ctgtgatgat
    40921 catttcttgt ctttcaaacg tttctttagc tattttatta tcatggaatg tgtatagtcg
    40981 atttgtcgtt ccagaaatca tgtataaaga gagtccattg aatgacgtta gattttctaa
    41041 aatcatatct agttctttct tttgaaaacc aataaaaata cttcctgact cagctcctcc
    41101 ttgtgtgata ggcaacttga tataaaaggc gtctagtaat tttggtgtcc ccttttttat
    41161 ttccccacct ttgttttctc tagaagattc ataatactcc ggtagttgat tcaacacgat
    41221 atatatcgca cttagttctt cataatttgc gtagagatct ttgatttgat ttgggaaaga
    41281 aactaattca tcggtactgt tttgctgttc gtatacatac atcagatact gatctatggg
    41341 ttttgttaag taggtcgtca cattttcgat cttttcggaa ttccctacaa gctggttttt
    41401 gatgatccta ctcaaggttg ttttatcatt cagcattctg cttgtgcttt gaacggcatc
    41461 acttgtcatg ttttctgcct gtttttgatt ttgttcccaa gtatggatac taaaaataac
    41521 ggtagcagtc atcgttacac cgatcatgat aaccgcgtat ttccgcaaca gacgatttag
    41581 taaggttcct tttgaaaata gaatccgaaa cttattcata actttccaac tgtttatcta
    41641 attcttcacg ccaatgtgtt gtcatcgtac cattccaaac cgtcaaaaga ccaatgacac
    41701 caaacaggat caatcctttg aattgccaag tcacgcctaa aattataccg ctaccaataa
    41761 ttatttttag aaaagacgaa aagctggaaa aaaccgaaat aaaacttagc tttagcagat
    41821 taggaacaga gatcgtatat tcactatcta aaatcatact gtattgataa gtagcaaata
    41881 ggcagagcaa ggcaaaaagt aaaataaaat caatagctaa aaaaatgagc cctttgattt
    41941 gaacagataa atataaattg taactcaata aagcagtacc agataaaaat aaactaaata
    42001 gcagattgcc tcttaaaaaa tttcttttat atatattcca gcctcttttt attgtgattt
    42061 ctttatactc gaatccgtac aaataaaata gttcattgac tgttttccat gccgcaccta
    42121 atccaaagat gatcccgccg gcaaatgtat atatccagaa atacaaggtt agttttataa
    42181 tgacccagat ccggataaat aactcttcca aaaccttccc taacatatct tctcctccaa
    42241 acattaagtt tatactttct cattatacaa aaaaaggttg ggcaatcagc accttgacct
    42301 gattattaga gaaacaaacg gaaaaatagt tcctcatatt ttgtcgtttt gtgagcgaat
    42361 accgagggtt agctgattga acaccgttta ccaagagttt gtgaaatagc cgcttagttt
    42421 cgagtagtaa gaaaaatttt gtgaatatgg ctttccacat ttttacatat atttagctta
    42481 ataccgagaa actggctttt gaacaccgtt tattcggaac aagaggttgt gacacgtctt
    42541 ttgtcacaac ccctttgtct ttattcatta ttattcttgt cggaactcat catattgttt
    42601 ttgcatttct ttcaatacct tatcatagcc agccttgtcc aaagcgtctt tcaatttcgg
    42661 taatgtttca tctggatcaa cagtccctgt attcaatcca tctaaatatt gattcatgac
    42721 attagaaata ttacttaatt ctgttttgac actgtcagtg ttgaagctga atcctaaaat
    42781 cggtgaagtt tctgcatcag caatcgattg atcacgtttg gcaatcatgt catccgtaat
    42841 agactcttgt gtatatagaa tcttgttgtt tcctgtattc caagcagaca tatgtgtatt
    42901 tggctgataa ccatctagca atttgatttt atcatctgaa ccatcgactt tttcccatgc
    42961 ttctccctcg atcccccaaa caagcccatt caataattct ggatcactgt taagcagccc
    43021 tagaagttct actgattttt ctttattttt tgaagtattt gatacgacaa aatttgccat
    43081 ttgtgcttga gctgtagatt ttagtggaac agtaattgct tttgagacaa gttcttgttt
    43141 tgctgcattt gtcaaaattg tatcgccata atcatacggt ccttgtgttt ctccacgcat
    43201 tagccaagta tttccttcta gtggataatc cttattgctc gttgcagcgt cacttggaat
    43261 gagtccttgt ttgtaccagc tatgcattgt acgtaacagt ccgatatacg tttcattatc
    43321 gtattggttg atgattttcg aatcatctcc atccagatca atagcaaatg gtaacgtatt
    43381 ccctaaagga taatcaaagt tcccttggac tttgaagcct tgtccaatgg caaaagctgc
    43441 cgtgtttggt tctttttcat ggaattcctt caagactttt tctgcatcag cgtaagattg
    43501 gattccatca attgaaagat cgtatttatc tagtaaagct ttgttaaaag taagtacttg
    43561 ttgagcaaag acatttccat tgactgggaa agcatataat tttccattga ccagatttcc
    43621 tttaatatat gcttcatcta aatcattata agctttttca gcgtacttag gcgctaattc
    43681 agtcaaatca gcaaaggctc ctttttgggc attggaaaca tagttgttcg caaaggcgat
    43741 atcataattt tccccggaag aaatgatgac gttcattttc ttttcgtaat cgccccagcc
    43801 aatgtaattg atattgactt ttacaccgat ctctttttcg attcgtttgt ttgctacttc
    43861 catcaattga tcgaaattat ctggtttatc tccgatttga tacatctgca aagtgtccga
    43921 attgctagct tctgcctggt ctttagaacc agaacctccg catgctgcta atgttcccaa
    43981 caacaacgct gaaacacctg taaaggctag tttcttccat aacttcattt ttcttcctcc
    44041 taattttatg atttatattt ttgtttcttt attcttttac tccgccgatt gttagacctt
    44101 tgacaaaatg cttctggaaa aatggatagc tgatcgcaat aggcaacgtc gagatgacta
    44161 cgatcgccat tctggcagat tctcctggaa ctgaggctaa tccgcctgtc aattggcttc
    44221 ctgctcctga attttgtgtc agatattgaa tattactttg gattttcatc aaaagatact
    44281 gtagtggaac tagcttatca tcttggatgt atagtaaagc attgaaccag tcattccagt
    44341 aacccaatgc ggcaaataaa ctgattgtcg caatacctgg aacagctaac ggtaaaacga
    44401 tctgaacaaa tattcgcagt tcgctagctc catcgatccg tgccgattca ataatcgaat
    44461 cagagaccga acgtttaaag aatgtgcgca tcacaataat attgaaggga ctgacagcca
    44521 ttggcaagat caatgcccaa atcgtatctt ttaactgaag aagatttgtc ataacaagat
    44581 aattggccac catccctggt gagaacaaca tcgtaatcaa acaaaaaacg gtaaaaaaac
    44641 gacggaatgg aaagtttgac cgtgaaatcg cataagcata taaagaagta gccgtgctat
    44701 tgatcagtgt acctaaaaca gtaacaagta cagtgacgaa aagcgcttgg aaaattttat
    44761 cctgcatctg gtcgaacaag tacgtataac caaatatact gaacttttct ggccaaaaac
    44821 tgtacccgtg agtcgacaat atggactcat cggtaaaaga gatggcgata acaaatataa
    44881 atggcaaaat acaggaaata gcaaagagtg caatcattat attagagaaa aaattagccg
    44941 tcttattgaa agagcggact tggactctct ccactttctt ttttttcatt tttttcctcc
    45001 ttagaataat gcagaatcgc tatcaaaacg tcgggcgatc gtatttgtga tcatcaacaa
    45061 gacaaagccg acaactgatt ggtaaagtcc tgcagcagcc gtcataccaa aatctcctgt
    45121 ggatgtcagg ccattgtaga tatacgtatc caaaacttga gtcacatcat ataatgcacc
    45181 ggaattccta ggaatgttat aaaacaggcc aaaatcagct cggaaaatgt ttcctacggc
    45241 taaaatcgtt aaaatcgtca ttagtggaat aagttgtggt atcgtcacat gtttgatctg
    45301 ctgccatttg ctagctccat caaccatagc agcttcataa taagtcggat cgattcccat
    45361 aacagatgca aagtaaataa tgctgttata accaatccct ttccatgtac caatgaacac
    45421 caagataaac ggccaatatt tcggttcatt ataccaattg acagcttctc ctccatgtcc
    45481 agtgatccac tgattgaata ttcctttgtc aggacttaaa aaagcgtaaa caaagtaact
    45541 gataatgacc caagatagaa aataaggtag taaggacatc gtttgataaa ctttaacgag
    45601 tcgtttattt ctcaactcgc tcataatgat tgcaaatacg actgagatca gcagattcaa
    45661 aaggataaat gctacattat ataaaattgt atttttagtg atgagaaatg cttgatccga
    45721 agaaaataaa aatttgaaat tttcccaacc cacccaagga ctttccctca agctagccaa
    45781 gaacccatct gctgaaatat gaaaattttt gaatgctacg acattcgcca ttacaggtat
    45841 gtaaaagaaa aaaatcatcc aaagcatacc cggtacagcc atcaggacaa gtgctttata
    45901 acgccataga ttcataaaaa aatgattgaa cccctttcgt tttttcatct aaaaacccct
    45961 ttcgattcat tcgcaacgta attatagcgc tttcaataat gagaataaat aaacaaagta
    46021 tagggaaaat gaaacaaagt ataggagagg ttgtgaaatc agcgttttgg cctgagtatt
    46081 agagaaaaaa acgggaaaat agttcctcat attttgccgt tttttgagct aataccgagg
    46141 gtcagctgat tgaacaccgt ttatcagagg ttgtgaattc agcgttttgg cctgagtatt
    46201 agggagatcg tgtccttact atctaactct gagtcacaag gaacaatggg gactgtaaca
    46261 aaagtcttgt cacagtccct tattccgaac aaacggtgtt ccagaagtag cttctacgga
    46321 aataagccga aatttcacaa aaaagccatc cgaaagtcag acggacaagt ctgcttttca
    46381 gatggcgcta caactactct gcttattctt ttagctatcc attcaattat ttttctctca
    46441 cttgttctag ctcttctttg gtcatgggtc gatattgccc tttattcaaa tctttaggca
    46501 gccaaaattt tcccatacgg attcttttta aatataagac ttcttttcct actgctttga
    46561 ccattcgctt aacttggtga aactttcctt catgcaaaat cagacggata attgaagttt
    46621 ctgtctcttg atctacttga tcaatttgga gttttgccgg caatgtctgc tcatcgccat
    46681 caataatcag tccttctgca aataaacgga tatcttcttc tgtcattata ccgctaacct
    46741 cagctagata ttctttctcc acatgttttt taggtgatag taactgatgg gcaaacaccc
    46801 cgtcattcgt taaaatcaag aggccctctg tgtctttatc taatcggccg actggaaaaa
    46861 gatcttcccg ataatcctgg tcttccaata aatcgattac ggtttgatca tgattatcct
    46921 gagtagcaga aatgactcct gcaggtttat gcaaaatgta gtaaaaatct ttttgatacg
    46981 taatgatttc tcctaaataa gtaaccgcat cttcgaattc atttacttgc gctttatcgc
    47041 ttttcaccac ttgtccattg acttcgatcg ttcctttttt gattaatcct ttgacttctt
    47101 tgcggctgcc aagtcccata tctgctaaaa acttatctaa acgcatgcgt ttcccccatt
    47161 tcaaaagagc atcaatctag ctcttctgat tcacttattt tatttttaat ttatgtcgta
    47221 aacgcgctat tcctggtcct agtagtcttt ctgccaagcg gattttcaaa gctaaataac
    47281 catacactaa agcccctata cctgctacta caagaatcaa caatagagac tggaatttgc
    47341 tatcttcgtt taaaaaagca ccaaaaatca tctttgtgat ccacgaagcc aaaagcataa
    47401 tcaaggtaag aataaagatc agcaatgtcc tgcgccagac aaaacttggt ttaaagcggg
    47461 ctacttcatg gattctcttt aagatcaagg tacaagacac agcaaatccg atcattgtcg
    47521 ccaataaagg gccgtatact tcaaataaac gaatggctgg gtattgaaga aggagtttca
    47581 ctagaaaccc aataaccaaa taattaattg ccgccttgtt ttcaaacatt ccttgcagca
    47641 tgttggaaac tagcatatat agtcctagga agagtcctac aaaacttgct tggatcagta
    47701 cattacttcc tagagaatcc ggtgtataaa ataatgtatt tagcggatag gcaagtaaca
    47761 taacaccaaa tgtcgccgga aacataaaaa atgaaaacaa ctgtaaattt ccgcttgtca
    47821 attttgccag acctactcga tctttcaacg taacagcttc tgtaatcaac ggtaacccag
    47881 tagacgcgat cgatgttgcc aatgcaataa ctaccatcgt caacttatct ggattggcac
    47941 taaaaatcga aaaaagatcc agtaactgtg cattggaata ctcggtggat gcagacatga
    48001 tcttcataaa agtgaactga tcgactaatt tgaagatcgt tacccctgaa cccacaataa
    48061 taaacggaat cgcttcttta acggtatcta acaacaattc cttcgttgca atatttattt
    48121 tgttttcgct gtagtgaata aacgccgatg tatacgcttg atgtttcttc aaaaaataca
    48181 aaaggacaag aatacttgct agcataccga taaatgccgc gaaagttgat tgcgtgaccg
    48241 ccgtcacata gtcaccatcc atgactttca tgatgatgaa tgtagccaat agcatataaa
    48301 aaatacgtgc cacctgttct acaatctgcg acagcgcata aggcatcatt tcttgattgc
    48361 cttgcaaata ccctcgtatc acactcatac aaggaaaaac caatacagcg atactcaaag
    48421 aacgcatcgt tggaatcagt tcttctccgc caccagaagc atgagctagc caaggagagg
    48481 cgatgtacat gaacaaagca aacaacaggc ctaacccgcc catcatctgt aatgcacgga
    48541 taaataatcg tcgtgaagtg ccatattcat tcaatgaatt atagcgtgca gtttgttttg
    48601 caatcgcagc aggtattcct gctgtagaga tcagtagaaa caatgcatat atattgtatc
    48661 ccatattgaa taatccattg gcagccttag cattttcgcc catccaagca taccagggaa
    48721 tgatatagac agcgcctagc aatcttgaaa tgatattact ggcggtcatc caagcagagc
    48781 ctcgtgccat tttttcttgc ggtgttaatt gatctaatgc tggttttctt tgattatcca
    48841 tcggtcagat tccaactttc tacaaaacat tactatcatt ttaattggta aaacagaaac
    48901 tggcaatcga ttttatataa cttttataag aacaattgtt tttttgaatt cggtttcgtt
    48961 tttttggtaa agtaaagaaa tatacagcct tttatggcta acatttatag gagatatcaa
    49021 tgacacaaga aataaaaaaa ctacccgtta tatttttagg tcttttcttt atcacagttg
    49081 gattgaattg gttcttgctc cctcacgata tagcttccgc cggagtgggc gccatcggac
    49141 atttagttga aacggccttt tctatccaac gctcagttac tgtttggagc atcaatctta
    49201 tgatgctgct tctagccgct gtcctattag gaaaatcagt attttttaag actgtagcgg
    49261 gaagtctgct ttttcctgtt gttttagaga ttgttccaaa agtggaaatg atctcctcac
    49321 atttcttttc actgatttca ggaagtcttt tgttcagtct tggggtttac acattgtaca
    49381 cagtcggggc ctcaaatggc ggtataacga taccgccgat tattttccac aaattttttc
    49441 ggcttccaat gccgcaagga cttttactta ccaatagtct tatcgtcttt ttgaattatt
    49501 atgtttttgg attgttggag acactattcg ttctcctatc tattacagtc agttcattgt
    49561 ttatgaaaat actcatccgc ttgtcccctg tgtcaagaaa atttgaataa agctggattt
    49621 tgtcatagcg gaaacaaacg atatattaaa aagagtgtgc tataataaac caagaaattg
    49681 aataaagcga gggatcaatc aatgagcttc tctttagaag acatccgtca tttactatta
    49741 aaagaaaatc tattaaaaga atttgtttca acaagaggct ggcatcttga tgtaccagaa
    49801 aaagctgaat ttcttcagtt aagttatgat tcaagaaaag ctgatgcttc tactttgttt
    49861 ttctgtaaag gaatgaattt caaagaagaa tatttggatt cagcaatcga acagggtatt
    49921 caatattatg tatcagaaca accatacgaa aattcagcag taggaatcat cgtaacggat
    49981 atccgtaaag caatggcgct tcttgctatg gctttttacg actatccgca aaataaattg
    50041 aaagtgatcg gattcactgg aacaaaaggt aaaacaactg ctgcgtactt tacaaaagca
    50101 atcttggatc acacaacaaa taaaaaaaca gcattgcttt ctactatgaa tacaacgtta
    50161 gatggcaaaa catatttcaa atcccatttg acaacgcctg agtcattaga tttatatcgt
    50221 atgatgtcag aagcagttga aaatggtatg actcatctag tgatggaagt ttcttcacaa
    50281 gcttacaaaa cacaacgcgt atacggcttg actttagatg tcggaatctt cttgaatatc
    50341 tcgcctgacc atatcagtcc gatcgaacat ccgacatttg atgactattt ctattgcaag
    50401 cgacaattga tcttgaattc aaaaacagtt gttttaaatc atgaaagtga ctattttgat
    50461 ttacttaggg aaacagccga tctattccag attccaacga tcacttatgg tcgttcggaa
    50521 gaaagcaatt atcaagtgat ccgttcagat aaaggtactc acggttttac tttatcttct
    50581 catgaagatc gcttagctat ctgcgatact gcttatgata ttttgctggc tggaggattc
    50641 aaccaagaaa atgcagccag tgcgatcatt gccgcagcac taacaggtgc ctccagtcaa
    50701 gatgcacaag acggattaaa agaagctcgt gtaccaggcc gaatggatca attgatccaa
    50761 tccaatggag cgcatgtcta tgtggactat gcacataatt atctaagctt gaaaacattg
    50821 ctgaaatttg ccaaaaatga acatccagat ggccgtgtaa ttgttgtatt aggcagccca
    50881 ggaaacaaag caatctcccg tcgacatgat tttggaaaag tgctatcaga aacagctgac
    50941 atcgcctttt tgacggcaga tgacccagca tttgaagatc cgaaaaaaat cgcggaagaa
    51001 atcaatgaag caattactaa tccagatttg atcgttcatt atgaaatgga tcgtcctgaa
    51061 gcgattcgac gcgctttagc agaaagcgct ccgcaagatt cagtcgttat tgcgggaaaa
    51121 ggcgtggatc cctatcaaaa aatcaatggt gtagatgaac cttatgaagg ggattatgca
    51181 atcgtgaagc ggttgattga agaataatgg tatttatata ctagtggttt cccatttcta
    51241 agaaaaaaag ggactgtgat tttttgtcac agttcctttt ttcttagaaa tcattttaac
    51301 ttttcactag ctaattctgc tgccttttct ctttcttcat ttaataattt attatcatag
    51361 aatttgataa atgggaacca gataatgaat gcgatgattg cacaaatgac tgctaacaca
    51421 gcagctttcc aatcaccacc actaccaaca aatgctccga tcccgactgg tgaaggccac
    51481 ggcatctgtg caagcatcgg tttgacgatt tccagccgaa tggcaaaata agcgagtgta
    51541 gcagaagcca tcggagctaa gaaaaacgga accgctaaat agggattgta gacgattggc
    51601 atcccgaaaa tgattggttc attgatattg aataatcctg gtacaagtga ggcttttcct
    51661 aacactttca gctgatcaga tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc
    51721 gcacctgatc caccaacggt tacataagaa ttgttgaatt ctcctgccaa cggataattt
    51781 gcgccttgtg cgttagctgc catattagct aatacgatcg gggtcaagaa agaagtgatg
    51841 atattcgctc catggatccc tacgatccac aatgcatgga tcaagaaata aatcaccatg
    51901 acaccaagcc atgtattcgt caactgcgta acaaacccaa atgggatagc aatcattttg
    51961 aagatatccg ttcctagtgc aactaataat ccattgatga tcatcaccac aaacgcaatg
    52021 acaaatgttg ggatcagagc agtgaaagaa cgagaaactc ctggcggaac aacatctggc
    52081 atttttatga cccaatttct cttgacacac atacaataca attcgaccgc aataatcgac
    52141 ataataatag ctgtaaagat tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta
    52201 ataccattta ctacagtttc tgtctcattg atagaagcaa tcaagctgat tttaccatca
    52261 gtcatgatca attcagggat acacataaag aatgcaaaaa cagatagtaa tgcccccgtg
    52321 agcggattaa catttaattt ttgttcatct cgttcaattg acgttaattc ataagcaaac
    52381 acaatggcaa agtaaataga taaaatcccc atcgtcattg tattgacgat catgtacaag
    52441 tcactaactt tgaaaaaagt tgcattaaaa aatccttcta aaaatgggaa cggtaatggc
    52501 aaaatattta aaaccaaaaa catcgagcct acaatcgtga aaggaataga agccatcccc
    52561 gctgccatga cagcccgaac gattttaagt tgcgcaattt tccccatagg tcccatcagg
    52621 tatttctgca aaaaatcaaa cattagttat tctcctttcc taaatataac gctttttcat
    52681 attgtttaat acggatatac tgttggtctt ggttgagtga aattttcttt aaacgatact
    52741 gtataaatgc actggcaaaa ataccgctta caacaagaat gaaaacaaat aactgtcctc
    52801 cattgttatt tgccataaaa ggatacaaga atgtaaatcc tgaggtaaac aacactggaa
    52861 tcactaataa attaaagaca ctctggaaca aaaaatacca ctttgtccat ggaacgttcg
    52921 tttggtgttt tcgataaagc tttacttgtt ctcctactgc aggcaaaata gctaaaatca
    52981 gaatcatcgg taaactactt cctaaacttt tagctgatag aagcagaatc atccattgca
    53041 tgttcatgaa aaagaaaaag gcagtcaggt aacgtacgat tagataacga ttgaaataca
    53101 tgtttttcaa actaagttgc tttttttcta attcgatttt cctttttact gtatcctgca
    53161 caatgatcct tcctttctat ttattttttt gatacaaata attcatttcc tgtgcaactt
    53221 ccaataatgt catcgtcgtc atcagatgat cttgtgcatg gaccatgata atttccattt
    53281 ctatttttgt cccgctggca tattctttca gaagttgagt ttgtgactga tgcgcatgaa
    53341 gaatctcttc atttgcttgt cgcaacttct cttctacttc ttcaaacatc tctttacgca
    53401 tcgagcgaaa tgcttcatgt atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa
    53461 aagaagtcac ttgtagtgtt tcactgttta ttttttcagc catctgtttt cctccttatt
    53521 cgatcagttt gataaattta ctgctgaaat tctcaaaaat aggttcagct aagatttcct
    53581 gctgtacttt tagctggtca actaacctaa cgattgcttt agtcacgacc gtaattcctt
    53641 cattttcgac ataagaaggc gaaactaaaa aaacaaaatg aatttcttga tattgatccc
    53701 actgcatccc agaaggaatc aatgcaacgg ctacctttgt ttcgattcca actggaacag
    53761 cgggatgtgg gactgccact gtatcactga atatgatttg tcccatattt tcacgttgct
    53821 ggatctgctt tcgcatttct cttatatatc cttcttcttc atttatgctc agcttttgca
    53881 gcaaatcatg aagaatttct tcctttttcg gttcgtacgt ataagtcttg aaaaattttt
    53941 cagacaactg ttcggtgtaa atctgctttt tttgctgtag agaaaatgct ggtatcggtc
    54001 tatctgagga atgatagggt atagattctc caactacctt tcgtatttta cgaacatcat
    54061 cttcattcaa aaaaatactg acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat
    54121 caatagaaga aataatcagg tcgattcctt ttaaagttgt ttcattgatc tcataatagc
    54181 cttttacact taccactgta atatttttac cgaattcact gactacgcga ttctttaata
    54241 gttgggcact gccgtaaccc gttgcacaga taatcaatgc atgtagttta tgcgcagctc
    54301 tttctttttc caatgccgcc atgagatgca aagccaaata ggcccattca tcttcattga
    54361 tggtatagtt tttcaaagcc ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt
    54421 tgggattttc tttctttatc tcctcagtca atgggttctc aatgcgaata cctcggctca
    54481 atcgaatcat catcggtgac aaatgatcga gtagtccatt gaaaagctgg agatcctcta
    54541 ctaatgaaaa atgaaattcc tgagacaatc ttttcagaac tttcgctaat tcctttgata
    54601 gttcctgatc attttgattt tctttgtgat tcgacttagc catgagatgt aatgtcaaat
    54661 aagcaatttc ttcttcggga aacgatacac ctgcgattac ctcaatacgt tgaacgatcc
    54721 gctgcgccac ttgatactct actcgcttag aaacttcttg ttcgatgcct aattccttga
    54781 tttcaaaccc ttctcggagc cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta
    54841 tgataaaatc cgaaactttt aacttagctt ctcttgtttc atcgagaatg ataatgacta
    54901 gctcttcaaa actgatgtct gggaaaaaac ggctttgccc taaatattct ttcaatgaat
    54961 ttccataatg gtttccaaaa aaagtatcca tgataaaatg ccgtcgattt ctttcttccc
    55021 cttcgatcca tgtcccttga ccatgcttag aaacgatttt tagtgaatat ggaatcaatc
    55081 gctccttgat ttcttgcatg tcctttgtca aactggaacg actgatataa agctcttctg
    55141 ccaaatcatc cataaaaaga atggcatctt ccaaaagcaa ttggttcaat atatactttt
    55201 gtcgatcttc ggactcataa aatccttgcg gttctctagc atatccaggt actatttctt
    55261 gccgactcaa aaacaagtca aatgtcagtc tttgtaaaat atgcagttgg tagccataac
    55321 ctggtttagc aatgatctta cctccgtttt tttctactaa ctctttgatc cgctgcaagt
    55381 aattacggat tgtccgatca gataataaca attcagaagc taaacgttga ctggtgacat
    55441 actccttctt gtgatcaatc aaatatctca acagcaattt ttccttctgt ttcattatca
    55501 tccactccta ttccatcata tgttctcttc gatcacctct gctaacttct ggatgcctgt
    55561 agggattggg acatacgctt ggaaagggat actaacaaca ggctttccaa cagattctcc
    55621 taattttttg aatttatcca aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa
    55681 atcactattc ttaatcatat tatcgccttc tgtagctgaa actgcatcta cgagtacctc
    55741 ttctcttttt tgcttaaaga aatccgttgt ctttgaggcc atcatcgagg atgacattcc
    55801 tgccgcacaa ataatcaatg cttttttcat aaatcaatca ctccttttat ttttataaac
    55861 caagcatatc ttgcaagcgt tttctattca aaatatatat ttccgtagca ttgcggaaaa
    55921 aatgtgtatc atatcttgaa taataatttc tggcaccgtg atatactagt gatagaattt
    55981 tttcaggagg ggatttagtg agtatcacag tgaatatttt gtatacaagt acaaatggac
    56041 aagccagaaa atttgcagaa gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag
    56101 aagaaggaaa cgagaaatat gactattact ttccactaga agatccagaa tctcttttac
    56161 tgatcgaccg ttggaaagat gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg
    56221 ctcaaataaa cgaactccgt aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg
    56281 atttaccgta aaaagataca catggaatga tagcaattta tcatccatgt gtatcttcac
    56341 tttttcctta ttttttattc gatctcatct aatgattcaa accgtgtttt tctcttattt
    56401 ttcggataag tatttcagtc accccaatat acatgattcc taaacatacc ccaaggatat
    56461 tctcaaaaat gcggatcgtt actgctcctg gaaccccgaa taaactggca gtgattgcca
    56521 aagcaccaaa agaattaaaa actgttttat atctataggt agaacaaaca cctaaagcta
    56581 gaccacctaa tataccgacc cagttaaaag gaataaattg gctgatacca ataaataaaa
    56641 cacatccaat caatgagcca atgattcggt ctactgctcg ttctttgaaa ccaatcgaca
    56701 tcaatccata gctagataca atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa
    56761 aagggacata ctctcctgca acgatcaaaa gactaatgcc aaatgcatag atgagcatcc
    56821 aaattttttc ctgcgaatac atgcctttca aaaaaatctc tttaaacaag cttttgtctt
    56881 tgttcttttc tcgatgtttt ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata
    56941 ataagctact tgtttgtgta aagaaattct tgttcaacga atctttaggt aacgaataaa
    57001 caataaagag atatgaaaag ccgtacaaag aagcattccc cattttggga tcactagcgg
    57061 ttgccattaa tatgctgctt agtaaaataa aatgaatcaa catgcccata actgaccatt
    57121 tgatcagctg gacatacggt gctactaata gtgagaacat gacgattgct aaacctatga
    57181 tcgaatggga aatcttataa ccaaagtcaa caaaacgaat actcatcaac atacagaata
    57241 aaaccacagc gtatggttta tgcttcgatt caaataatgt tgtaattata atgataaaac
    57301 aaatggcaaa caatacgatg aataatgatc gtaaaaacaa tgaacaaata tacatgcact
    57361 tttctttttt agaagctgct tctcgtattt tttgttttaa aataaacgga tctagttgta
    57421 gagactgata aaaatccacc tgttgtcgtc ctttcttttt acttttattt tttacagtca
    57481 tctatgacag gttgtaaata ctatggaaca tcatatggcg ttgtcatttt cttgtcaact
    57541 atttttccta ttcatgtttt tataaaaaaa catatcgttt tcttgtatga ttaaatcacc
    57601 acaaaataaa caactgagag aacgatatgc tttatgaatg gttctatcaa aaaaattctg
    57661 agatcaatag acaaaaattt gatgattaca gaggtctctt acgaaacctt acagaagaaa
    57721 aaaacattga tcgttgctgc agttctcttc cctgctccat gtgtctgtag aaattgtggt
    57781 tctgctgtgg tagatggaaa taggaaatta atggtggtga aaaacaagaa gaaggaaacc
    57841 attgtccact ttgaataata taatcatatg ccaatggtta tacgcctaaa aaagcagcgc
    57901 tatacctgta aaaactgtcg aagccattgg aacgctcaaa gttattttat ccgtccaagg
    57961 cactcgattt caaatcatgt cagacataaa atcacttctt tactgactga aaaagtgtcg
    58021 ttatttttta tttcgaaaag ttgttaggta tctttgacaa ccgttattcg cacattgaaa
    58081 gagttgagaa gctatttacc aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt
    58141 gtgaatttcg ttctcacgtt tccgttgagg ataaactgag ttttatttcc gcggatggcg
    58201 agacagaaaa attaatcgag aaaatttacc tacacaaaaa attcctcgat taacaaacta
    58261 tttccttagc tgcactaatc ctaaagaaat agaattctta gttacagaca tgaacgccac
    58321 ctactttcag ctcaccaaac atgttctacc aaatgcgaaa ttggtgattg atcgattact
    58381 tggtttttcc acgcctttca aggaggcgta acctttattc acgagttggt tgaagctttc
    58441 cgagacaaaa gacccgactt gtttttctcc ttattgacaa agagccatta aaactagaga
    58501 aaaaattaca actgatcgct gattatttgt agaaagaaaa tcaaagtatt acccaataaa
    58561 cttgagtcat taggcaaaaa acactgcgaa actcaagaat gattttcgca gtgtttttgt
    58621 atatttttta ttaactagta ttactacaaa ggtttatttt tcacttctca acgcagcaca
    58681 aaattgtctt tacttattta ctggttgggt tcaaccttct ctactccgat actaatattt
    58741 gtttgtatac cccttctaaa atcggtagtt tccattcaat tgatgggtac tagttgattt
    58801 taaaaactat gcttattcaa ttttttattt tagctcatat atttcgtttc tttctaaatt
    58861 ttatactgat gaaaacaagt agcacaccca atattgacaa tataaactgg ctaatgatgg
    58921 tttctccagt ttttggaaga tgcttatttc cactgcttgt tggattgttt cctcttatat
    58981 ctgagctatc agcaactttt ctattctctt ttgttaaaaa gatcgccact tcttgttctt
    59041 tttcgatctt gaaagttact ggtgtcgcat ccaattcata ccccgttggc gcttgagttt
    59101 ctaccagctg atatgctcct ggtgctaagc catctatcgc cagttttcca tctgctcctg
    59161 tggttagacc tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt
    59221 gcaggacttc tccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc
    59281 gattttcttt cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgattg
    59341 gtgtcgcatc caattcatac cccgttggcg cttgggtttc taccagctga tacactcctg
    59401 gtgctaagcc gtcaatcgcc agttttccat ctgctcctgt ggttagacct gactgcagaa
    59461 cggttccatt ggcatcttgt aactcaaaaa cagctccttg caggacttcc ccgctttggt
    59521 catcgatctt tgtcaacacg acaccgcctg gtgtaagtcg gtttgctttc gtcaattcta
    59581 ccaccgctgt ctgacttctt tcgatctcaa actcgatcgg tgtcgcatc
//
LOCUS       DAFELO010000008        56674 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFELO010000008
VERSION     DAFELO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.008"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            14..1141
                     /locus_tag="LP452_000586"
     CDS             14..1141
                     /locus_tag="LP452_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2013342.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1362..2504)
                     /locus_tag="LP452_000587"
     CDS             complement(1362..2504)
                     /locus_tag="LP452_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2013343.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2509..2694)
                     /locus_tag="LP452_000588"
     CDS             complement(2509..2694)
                     /locus_tag="LP452_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL2013344.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3048..3293)
                     /locus_tag="LP452_000589"
     CDS             complement(3048..3293)
                     /locus_tag="LP452_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013345.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3287..3688)
                     /locus_tag="LP452_000590"
     CDS             complement(3287..3688)
                     /locus_tag="LP452_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2013346.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3873..5681)
                     /gene="uidA"
                     /locus_tag="LP452_000591"
     CDS             complement(3873..5681)
                     /gene="uidA"
                     /locus_tag="LP452_000591"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBL2013347.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(5704..7470)
                     /locus_tag="LP452_000592"
     CDS             complement(5704..7470)
                     /locus_tag="LP452_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBL2013348.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7483..8277)
                     /locus_tag="LP452_000593"
     CDS             complement(7483..8277)
                     /locus_tag="LP452_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2013349.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            complement(8287..9663)
                     /locus_tag="LP452_000594"
     CDS             complement(8287..9663)
                     /locus_tag="LP452_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2013350.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            complement(9783..10430)
                     /locus_tag="LP452_000595"
     CDS             complement(9783..10430)
                     /locus_tag="LP452_000595"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBL2013351.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10523..11950)
                     /gene="uxaC"
                     /locus_tag="LP452_000596"
     CDS             complement(10523..11950)
                     /gene="uxaC"
                     /locus_tag="LP452_000596"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL2013352.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12024..13103)
                     /gene="uxuA"
                     /locus_tag="LP452_000597"
     CDS             complement(12024..13103)
                     /gene="uxuA"
                     /locus_tag="LP452_000597"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2013353.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13106..14737)
                     /locus_tag="LP452_000598"
     CDS             complement(13106..14737)
                     /locus_tag="LP452_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL2013354.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15043..15714
                     /locus_tag="LP452_000599"
     CDS             15043..15714
                     /locus_tag="LP452_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2013355.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQHGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(16417..16772)
                     /locus_tag="LP452_000600"
                     /pseudo
     CDS             complement(16417..16772)
                     /locus_tag="LP452_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16789..16956)
                     /locus_tag="LP452_000601"
     CDS             complement(16789..16956)
                     /locus_tag="LP452_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013356.1"
                     /translation="MQTLINALQGKNYDYAVKILLDVAKRSLTPAQFAKFNAATIAAG
                     IAINAISCWGK"
     gene            complement(16956..17363)
                     /locus_tag="LP452_000602"
     CDS             complement(16956..17363)
                     /locus_tag="LP452_000602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013357.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            complement(17864..18058)
                     /locus_tag="LP452_000603"
     CDS             complement(17864..18058)
                     /locus_tag="LP452_000603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013358.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            complement(18072..18410)
                     /locus_tag="LP452_000604"
     CDS             complement(18072..18410)
                     /locus_tag="LP452_000604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013359.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(18454..18786)
                     /locus_tag="LP452_000605"
     CDS             complement(18454..18786)
                     /locus_tag="LP452_000605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013360.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18800..19057)
                     /locus_tag="LP452_000606"
     CDS             complement(18800..19057)
                     /locus_tag="LP452_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013361.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KVYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(19252..19788)
                     /locus_tag="LP452_000607"
     CDS             complement(19252..19788)
                     /locus_tag="LP452_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013362.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(19809..20900)
                     /locus_tag="LP452_000608"
     CDS             complement(19809..20900)
                     /locus_tag="LP452_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2013363.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20908..23118)
                     /locus_tag="LP452_000609"
     CDS             complement(20908..23118)
                     /locus_tag="LP452_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013364.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(23134..25662)
                     /locus_tag="LP452_000610"
     CDS             complement(23134..25662)
                     /locus_tag="LP452_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2013365.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEYKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(25716..26126)
                     /locus_tag="LP452_000611"
     CDS             complement(25716..26126)
                     /locus_tag="LP452_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013366.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(26139..26357)
                     /locus_tag="LP452_000612"
     CDS             complement(26139..26357)
                     /locus_tag="LP452_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013367.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(26354..27346)
                     /locus_tag="LP452_000613"
     CDS             complement(26354..27346)
                     /locus_tag="LP452_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2013368.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(27425..28336)
                     /locus_tag="LP452_000614"
     CDS             complement(27425..28336)
                     /locus_tag="LP452_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2013369.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(28355..28699)
                     /locus_tag="LP452_000615"
     CDS             complement(28355..28699)
                     /locus_tag="LP452_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013370.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28719..29276)
                     /locus_tag="LP452_000616"
     CDS             complement(28719..29276)
                     /locus_tag="LP452_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013371.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(29356..29856)
                     /locus_tag="LP452_000617"
     CDS             complement(29356..29856)
                     /locus_tag="LP452_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013372.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(29857..30402)
                     /locus_tag="LP452_000618"
     CDS             complement(29857..30402)
                     /locus_tag="LP452_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013373.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(30399..31655)
                     /locus_tag="LP452_000619"
     CDS             complement(30399..31655)
                     /locus_tag="LP452_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2013374.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(32018..32332)
                     /locus_tag="LP452_000620"
     CDS             complement(32018..32332)
                     /locus_tag="LP452_000620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013375.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(32459..33652)
                     /locus_tag="LP452_000621"
                     /pseudo
     CDS             complement(32459..33652)
                     /locus_tag="LP452_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(33842..34198)
                     /locus_tag="LP452_000622"
     CDS             complement(33842..34198)
                     /locus_tag="LP452_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013376.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(34264..34716)
                     /locus_tag="LP452_000623"
     CDS             complement(34264..34716)
                     /locus_tag="LP452_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013377.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(34718..35083)
                     /locus_tag="LP452_000624"
     CDS             complement(34718..35083)
                     /locus_tag="LP452_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013378.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(35100..35294)
                     /locus_tag="LP452_000625"
     CDS             complement(35100..35294)
                     /locus_tag="LP452_000625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013379.1"
                     /translation="MEKKNINICFLSGKECTYEECTDISVHERDGMLIVDMSPSDEPF
                     VVKHYFPLCNIESFRIDCEF"
     gene            complement(35311..35556)
                     /locus_tag="LP452_000626"
     CDS             complement(35311..35556)
                     /locus_tag="LP452_000626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013380.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
     gene            complement(36272..40567)
                     /locus_tag="LP452_000627"
     CDS             complement(36272..40567)
                     /locus_tag="LP452_000627"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBL2013381.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            complement(40714..40953)
                     /locus_tag="LP452_000628"
     CDS             complement(40714..40953)
                     /locus_tag="LP452_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013382.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(41043..41315)
                     /locus_tag="LP452_000629"
     CDS             complement(41043..41315)
                     /locus_tag="LP452_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013383.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(41398..41649)
                     /locus_tag="LP452_000630"
     CDS             complement(41398..41649)
                     /locus_tag="LP452_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013384.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(42647..43558)
                     /locus_tag="LP452_000631"
     CDS             complement(42647..43558)
                     /locus_tag="LP452_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013385.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(43755..45590)
                     /gene="lepA"
                     /locus_tag="LP452_000632"
     CDS             complement(43755..45590)
                     /gene="lepA"
                     /locus_tag="LP452_000632"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL2013386.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            45737..46957
                     /locus_tag="LP452_000633"
     CDS             45737..46957
                     /locus_tag="LP452_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2013387.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            46977..47471
                     /locus_tag="LP452_000634"
     CDS             46977..47471
                     /locus_tag="LP452_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL2013388.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            47449..48135
                     /locus_tag="LP452_000635"
     CDS             47449..48135
                     /locus_tag="LP452_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013389.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(48286..50895)
                     /gene="clpB"
                     /locus_tag="LP452_000636"
     CDS             complement(48286..50895)
                     /gene="clpB"
                     /locus_tag="LP452_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL2013390.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(51146..52192)
                     /locus_tag="LP452_000637"
     CDS             complement(51146..52192)
                     /locus_tag="LP452_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL2013391.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(52203..52475)
                     /locus_tag="LP452_000638"
     CDS             complement(52203..52475)
                     /locus_tag="LP452_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL2013392.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(52510..53418)
                     /locus_tag="LP452_000639"
     CDS             complement(52510..53418)
                     /locus_tag="LP452_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL2013393.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(53550..54782)
                     /gene="pepT"
                     /locus_tag="LP452_000640"
     CDS             complement(53550..54782)
                     /gene="pepT"
                     /locus_tag="LP452_000640"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL2013394.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(54797..55918)
                     /locus_tag="LP452_000641"
     CDS             complement(54797..55918)
                     /locus_tag="LP452_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL2013395.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(55915..56616)
                     /locus_tag="LP452_000642"
     CDS             complement(55915..56616)
                     /locus_tag="LP452_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL2013396.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT        17146 a        11699 c         8460 g        19369 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aaaaaaaaaa
     1321 ataggctgtg acagcagcgt gacagcaaaa aagatggaaa gttaaatatc aaacctcgta
     1381 atttcatttg cgagtgcttg atttgtttct ggatataagt gtccatattt gtccaaagta
     1441 gttttaatag aggcatgacc tagacgctta gatattgcat agatattttt attatgttca
     1501 attaaaaagg caacatgcga gtgtcttaat ccatgcatat gaattggttt tactcctgca
     1561 atttcagcta atttttttat tctgcttaaa atagttcttg gagatggcgg taatccatca
     1621 aaagaaaaaa ccaattctat atttttccca attgattgtt gagttttttt ccatgttttt
     1681 aaatcttcaa cggtttgtct gtctaacgaa attatccgaa tacttgatgt attttttgtt
     1741 tcagcaaatt cataatcttg tcttgtacgg atgtaaattg acttatttac gctacatctg
     1801 ccatttatta agtcaaagtc tttccattgt aacgctaaga gctcctcact ccttacacct
     1861 gtaacaaaaa taaaacgcat caaacgttta taaaattctt cataaaaatc cccgtgataa
     1921 acactattat atactttctt aaattcatca actgtccaaa attctacttg aggacgctga
     1981 gtttttattt tacctatagt atctacagga ttttcttcaa taatttcaag tacaattgcc
     2041 cttttaaaaa ctactctcaa cttattgaat atctgtttaa tataatttgg tgataatggt
     2101 tgctcaatgc ccttttttac aacatggatt ttcgataaaa actgttgaaa ttcctgtata
     2161 tgaattggtc gaatctttac aacctgcatt tctccaaaaa aatccaatgc acgttgtaaa
     2221 atatgatctg ttttcatata tgttttttct acagttccca acttatacca aggaaaaaaa
     2281 tactctcgat aaaaatcatg aaacgttatt ccttgcagac aaattccttg tgaatcagaa
     2341 aaactttgtt ttaactcttc gagtcccctt ctagcttctc cttgagtttt gaagcctctt
     2401 ctggtcactt gaattctttt tccaaagcga tcttgtccta aagttgcaga aaaaaaccaa
     2461 gtgcctcttt ttctgtcttt ataaacattc gcaattttcc tcattttact attcctccca
     2521 agaaatatgg agaacttctt ctaaaattga aataggaatt ctttttattt ttgaattacg
     2581 ataaaaaaag tatcctttct caactaaaag ttctttacat tccttgaaga ttttattagc
     2641 atagtactga gaaaaaccta gattaatcaa atcttttcga gtagctgttt tcattatagc
     2701 accttcctta ctttttttca tttatttcta tcagctgctt ttgtcaaaag cacataggaa
     2761 tttcacctct ccccaagttt acggcgagcc ttacggaagt atcattatcc caactttaaa
     2821 tcatcgcttt ttattgacta taaaaattat tttagaatat ttatcgctcc ttctttacag
     2881 caagaaatca tggcgcaact aaaatatgct ccttaaagtg ttaacgtact gatagaatct
     2941 atttaatttt caatgaacaa taggttttct acctaaaaga atctactaat tccttaacta
     3001 ttagaagatt cttttaaaca gaaaaagtgg aaaggagatt tttttaatta gttaatttta
     3061 aattttaaga tcgcttctat taatttaatt tctagctgac ttatcatata ttcatctaca
     3121 taggtaacga attgacctac ctcgttaaat ccatttgtca aacataactt agccatataa
     3181 ctatgataat gtttgagtat catcttgatc gaaattggat ctccttctag agcagataag
     3241 ataatggata gtggaactaa taggtactgc cgctttttgg atttatctac cataggtcat
     3301 accccatttt caattgttca actgccttct ttcgttgata ccagatacca cttgtgctca
     3361 tattgagaat tttaccgatt tcagcatcat taaaacctgc aaaataataa agtaggataa
     3421 tctttcttct tatctccgat aactgatcaa ttgctgctga taacttttga tctgaaatta
     3481 atatttccat tccaaataag aaatagacat cttgttcatc aaaatgagtt tgggaatacc
     3541 tgccttcatt tgaatctgcc aatggttcct cattatcctt tatgttagca tttcgttttc
     3601 gaatagactt agcttcgttt cgtaatactt ttttacagta ggcatcaaat tggtgttgta
     3661 tagatatgtc attgtaatgt tgttgcatga taatatgctc ctttttcccc ctttccacta
     3721 atacaacaat cagagagccc ctttttttcg aaaaaactag aaaaaaacat aaaaatattt
     3781 atcattcttt aaacagaaaa tctttctata aaaaacaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaaatcca aattattttt tataactgaa attgggtatt
     3901 gatgaccatc gtttctgtag atagtgagca atcattttag gttttctatc tctagtaaag
     3961 attcctttct tatttccttg cactcttaaa atcccttgac tcgttgcaaa atctgcaaaa
     4021 ttccacactt gttcaccaac aaaattttca catgtatcta ttacttcgtg atttgcttcg
     4081 taataagcca cttgatactc ttctgtaaac attgttggta ctgtatcatg aaatccagct
     4141 actgtatctg ctccatattc tgtaaacatt attggcgtat caggacatct ttctttccat
     4201 ccatctagct cttttttcaa cagatatttt gcatgtgcca gatcccctcc cgaaacatac
     4261 caaccataat aacgatttaa acataaaaca tcgacaatat ctcctacctt acaaatatct
     4321 ggcgttgatt ttagataagt aactattgtt acaggtcttt tctgcggatc aagttcttta
     4381 gttaattcaa ctaaaggttc aaaatattct ttagcacctt cggaatccga atctggttca
     4441 tttgcaatag accacattac aacacttggg tggttcttgt cgcgttctat caattccttg
     4501 atcaccgttt tatgagaatt ttctgttcgt atctctttcc acgtattatg tttttccaca
     4561 ttatcatcta ataaagtggc catgaaattt aaatgtaacc ctactgcagg tacttcatca
     4621 ataactacaa taccttctcg atctgctaat cgcataattt cttctgagta aggataatgt
     4681 gatgttctaa aagaattagc acctatccat ttcattaaat taaaatcttt aatatttaca
     4741 gcctcattca aaccacgtcc attaacatac gaatcctcat gcttaccaaa tcctttaaaa
     4801 taaaaaggtt tattattaat caaaaatttg ccattggtaa cctgaacact gcgaatacca
     4861 aattcttctg tataggtatc gatcacttta ttatcatcaa ttaaactaat tttcatacta
     4921 tataaataag catctagagg ttcccaaaaa attggattct taagctgaat cattcctttc
     4981 tttccttcag cttcagctac aagttgagcc tcttcgtcat atatcgctat taagattttt
     5041 ccggtccatt ctgtatcgat attgaattct acacttgcat ccaaatcagt tattcttgta
     5101 ttaatagtaa tatcacttat atagtctttc ggagttgtat ataacttaac aggtctatga
     5161 atacctgcat aattgaagaa atcaaaatta actaagtttt ccttttttat atccccattt
     5221 tcctgtactg tttccttcat aatccccaca ggtaaagtac tatcgtcaat aatattgttt
     5281 acagcaactg taattcgatt attcccgtct tttacaaacc gattaatttc agactcaaat
     5341 ggtgtaaacc cacctttgtg ttgagtaatc aactttccat tcaaaaaaac ttttgcttca
     5401 tgtgttgcag atccaaaacg aagaactaat ctttcttcta ataatctttt aggaagagtc
     5461 acttcttttt cataccacac ccatccaaca tgatttctaa tgttctctgt ttcaaataaa
     5521 tcattataag aagagggaac tgaaatcgga taactctctt tttcatttat tggttctaaa
     5581 gcaagttctt cctgtaagtc atcatttgta tctaatttaa atctccagag accgcttaag
     5641 tcaattactt gcctcgtctc tgtcataatg ggatacaaca tataaaccac tcctatctaa
     5701 atattaaata aatgtatcct ttaaaccaca ataagcatct attggactta ctcccttaaa
     5761 ttcagaaaga cccattagtt tgtccaccaa tatttctaaa gtttcttgtt ttccatcata
     5821 agtattaata taagtcttta cttgaggtac atcagccaaa tgaaacggac actgtacaga
     5881 tacaaaaatt gttggaattt catgaatgta aaacggtata tccggagttc ctttacttgc
     5941 tggccagaca attcgttgaa ctgtgcctgg attaacatta gctatattaa tgatcaagtc
     6001 atatttttta gttaaatttt taattggtct cttagcagca tatattccag ctatattttt
     6061 aattttttct tcttgcgata atcctttcaa ctcttcttca ggtgaaatgt atttttcaac
     6121 ttcaaatcct tttgctgaca aaatctgttt caataaatca atggcttgag gtctatttcc
     6181 agcaactact tcaccaaacc caccttttac cccatgtaca tctactagta aaatcctttt
     6241 atatttttct ggttcaatag gccaaatatc ttgtttatct ttaactaatg taattgcttg
     6301 gtcagccacc tctttaaaaa ttgatttatt ttcaattaaa ccaatttttt ccagagcttc
     6361 ttgcttaggt aataaaattt gttcttttgc ttttttgtgt aatcccaatt tagcttttaa
     6421 ccctaaaatt ctttttaagg catctaataa ccgatcatca gagataattc ctttacgata
     6481 accttccatc atataattaa agtcttcatc tggatcatta aaaaatagaa atagatcaca
     6541 tccagctgca atagaagtgg gcaataattc tgatcgtttc attgccccag tcattcctaa
     6601 catatgacta gcatcagtaa ctactaatcc attaaatccg agttttcctc taagtaagtc
     6661 agtgattaat tcttttgata aagttgcagg aagtaaatct tcatctttca gatcaggatt
     6721 aaaatgtttt gaataagctg gcatatggat atgacctgcc ataatcgaag gtaatccagc
     6781 ttctattaaa cctgagtata cttttccaaa agaattatcc cattcttccg tacctaaact
     6841 attaatacta aaagatagat gctgatctcg ttcgtcaata ccgtcaccag ggaaatgttt
     6901 agctgcaggc aaaattccac tctcctgaat acctttcata taagctaaac taagttctaa
     6961 aactttatca gggttttgtc caaaagatcg actagaaatg ataggatttc tccaatttct
     7021 attaatatct acaataggag caaaactcca gttacatcca atagccgatg cttcaatccc
     7081 agaaactctc cctaattcat aagcatattt cgcatcattt gttgcaccaa tttttacttc
     7141 tacaccaact tcagtaccat ctgtacaagc tccgttacca cctgcttccg tattagcagc
     7201 aattaataaa ggaattttgc tttctgtttg taggatatag ttttgatcat atacctcatc
     7261 agcagttcct gggttatatc gtacagctcc aatatggtac tgattgacca tattactcaa
     7321 gtaattttta tcacgactag atcccatatt aacaaacaat tgacctattt tttcttccaa
     7381 agtcatagaa tcaatcgtct cttcaatcca agaaatagca tcttcatcta aattatatgg
     7441 tttttttcgt aagtctactt ttatagtcat tttctttctc ctctacttat tagtaagatt
     7501 tttccaaaaa gtatctataa atttttgttc aatatctaca tactctcccg ataaacattt
     7561 aaataaaaca tcagttctta agttttccca tgattctttt tctttattaa ccaaaatagg
     7621 ataaaagaaa actttatttt tatttgcagc ttctagatca ccaggagaat caccaatcat
     7681 taatatctta tccaaatcac aaccattttc aattacactt cgaataatgt cttctttttt
     7741 tcctttatct tgacaacaca gttcatcaac taatgataat aattcatgtc tttcccattc
     7801 ttcttgaaca gcttccttat tagctgagct tacaataatt atcttcccat attttttcag
     7861 agcttttata gtatctctag cacttaaaaa gggtttatct aatcctttag cttgactaat
     7921 ctgttgattg acttcttccg accattttaa agcttttaat aaatcctctg actgataata
     7981 ttctatttcc ctcttcaaag atggattaga caattctttt gttagagata cccagtgctt
     8041 taatcgatta atggatgtta catcattttt ttctaatgtt ttaactaaac ctaaaaatct
     8101 gtttattcca cgagtctctg aatataaatt aatattattc caatcctgta ggaatttttc
     8161 tctatgatga acatcaaaaa tctctgcagc aattggtccg aaaaagagat tgtgtttata
     8221 agtcatcgta tccataacac agccatctga atcaatacaa aatatccaat tattcatatt
     8281 tctctcctat ttatattttt gttttaaaag ctcgcaattt tctaagactt tatctttaga
     8341 taatggataa aggaaaaata aaattaatcc tacaataaaa aaacaaattg atggaattaa
     8401 tgttgaagcg ttatataact tttctaaaac atctcccgtt tgtatatttg ctaaagagtc
     8461 atagcctata atagaaagag tccaacctcc gacgcctcca gctagagctt gacctatttt
     8521 tctagcaaaa gaatataccg catatacggt cccatcttct ctttgatcag actttacttc
     8581 ttgatcatca ataacatcag taatatttgc ccaaataaca gtattaaaca cacccaaacc
     8641 catatagcca accgaactaa gaattaagaa tacatacata ttactagttt ttatcaaaaa
     8701 taagatcaaa tatactattg ctgaaaagaa cattcctact gatgccagtt ctttttttcc
     8761 atatttttta cttaaaaaca aagttaaagg tgaaacaact attaaaacca aaataggatt
     8821 gataaaattc attaaaacta ttccctgtgc atcattatag taatcaggaa atacataatt
     8881 attcatactc ataattaata attgagcaag gagtaagaaa atagccgcac caattataga
     8941 aagtaatgcc ctatttttaa ctaacccttt taaagatttt aacaatgaaa ctttgaattg
     9001 agattggtca attcttactc gttcagtagt tagtttataa catattaaat aacaaataag
     9061 tgcacatatc gaaaatattc ccgcaacaat tgtaaaagtt gatccaccac gaactatttg
     9121 agcgcctgaa tcatctcttg tataaataaa taatggtgtt aatacaccaa tgatcaaact
     9181 tgctaaagta gctccaacat ttcggaatgt cgatagcatt gttctttctt ctgtaataga
     9241 agtaatagct gatgccattg atccataagg aatatttata gatgtgtaaa aaatactccc
     9301 ccataatata tacgtaacat acatgtatac taccttaacg ctcatagaag ctgaaatcat
     9361 agaagtttga tacattaaaa aacttgctaa agcgactgga ccactcattc ttaaaatcca
     9421 cggtttaaac ttaccctgct tagttgcttt cgatctatca acaatacgcc ccatagttac
     9481 atcagtaact gcatcgataa atcttgctac taaaaataat gttccaacgg cagctccgct
     9541 tatccccaat acttttgtat aaaagatcat taaaaaagaa ctagcaaaaa tgaaagtaaa
     9601 atcgtttcca aaatctccaa acatatatcc tattttgtct cttataccaa attttctttc
     9661 catacaattc tctcccttct tttgttaagc gttttcattc ttatgaaagt acgtactata
     9721 ctctgaagcg taaagagtca gtatttattt tttacatttc agagtattat ttgttttttc
     9781 tcctaataat tctttatctc ataaaactta tctatatatt tactagctac tcgacttact
     9841 tcttcaaaat cacctttttc aacagaagcc aataaattcc cacctacacc aactgcaaca
     9901 actcctactt caaaccaatc ttctaagttc tctagactta ctccgccagt aggcattata
     9961 tttacgtgtg gtaatggagc ttgataggct tttacaatgg atggaccaaa agcagaacct
    10021 gggaatagtt taatgatatc tacgccagca gtaagtgcct cctttatctc agtaattgtc
    10081 atacaacctg gtaaataagg aatttgatat aaattacaaa tctcagctgt ttgtcgatca
    10141 aaagatggac taacgatata ttctgcccca gccataattg cgatacgagc agtaaacgca
    10201 tctaaaaccg taccggctcc gattattaca tctttttgat cagcatattt tttagcaatt
    10261 tctttgatta tttcttcagc attaggaact gtaaaagtta gttcaatccc tttaattcca
    10321 cccgcaataa ttgaatttat tgctttaatg gcctcatcct tatttttccc acgtacaacc
    10381 gcaatcactc cagccgcttc taacttactt aaaatttcta cttttttcat atctatctcc
    10441 tctatctcct tcttttatgg atacatactt tgtctagctg tactaactta aaaagaataa
    10501 atttaagctt ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat
    10561 tatacgcatt acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg
    10621 gaattcggcc tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact
    10681 catggcgagg atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca
    10741 acaaactttg ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga
    10801 accaccaagc acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg
    10861 ctgttgctag tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc
    10921 acccactctt ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat
    10981 cccccatcga atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat
    11041 ttcttaaagc atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca
    11101 aatgtaattg aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc
    11161 ctgctaaagc ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat
    11221 aagtattcaa accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt
    11281 ggctcaatgc ttgcttcaac ccattaaagt ctttgattgg tatagccgct gcttgactaa
    11341 gttttaaaat atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga
    11401 aagtaggtaa taccttgaaa gaattatcct gtttagaaag ctcagtatga taccccaagt
    11461 catctgtagg atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt
    11521 tcgggcgata ggaatccgtt tggatttttt cgttagcacg tcgccaaatt tctggtgcat
    11581 tcttctcaga aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt
    11641 caaaaatagg attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat
    11701 aatcatcacc tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc
    11761 atttataatg atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt
    11821 gcttattttc tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt
    11881 gttttgcaaa tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat
    11941 ctaaaaacat atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc
    12001 tctaaataaa ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata
    12061 ccatttaaat aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt
    12121 tctccccaaa tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac
    12181 attgttttta atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg
    12241 taaaaatcga gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc
    12301 cgatcccgtt gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca
    12361 atacttccag tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa
    12421 tctaaatcgt cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca
    12481 tctgggtgaa ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga
    12541 aaatatgcaa agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat
    12601 tgcttaattt gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa
    12661 ttttctactt ttctaataat ttcctccgga tcagtaggga tattttttga aatataggct
    12721 aacgtagaag acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact
    12781 ggcataaaat tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt
    12841 ttataattct caatgtattg atctcgtgtt tgtaaaccaa ttttgatatc atcatgaaca
    12901 ttaacactct caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat
    12961 ttaattcgtt ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt
    13021 cctttcattg ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca
    13081 taccatctaa aagtcatttc catagttact ttcctccaac ttctgttata tatttattga
    13141 ttgttttacg aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct
    13201 ttcctacccc aacttgatat aaatctaaac caaaaatttc tttattttct aaaatttcct
    13261 gtaccgcaca atgaatattt gaaggatctt gttcacctaa tttcaatact gataatttat
    13321 cctgcaactc taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga
    13381 ctgccagtaa ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg
    13441 catttccgtc tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt
    13501 gagaagtatc tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt
    13561 tattaattac ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg
    13621 gtaaaccttc taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct
    13681 ctgctgaaat tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg
    13741 gattcaagct tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca
    13801 taatcacacc agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta
    13861 aataatgaac tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa
    13921 tgatatctaa atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat
    13981 ttggcgtaat tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat
    14041 aagcaataaa tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa
    14101 tactatcttg aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg
    14161 gcaactctcc agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt
    14221 gaggattgct aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag
    14281 aatcttttaa actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat
    14341 cttcaaaata tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac
    14401 tatcagccgt tactgccaaa agcctttttt tgagttcacc atctccactc ataacaattt
    14461 ccaaaatgtc attgttgtag tcatgataag ctttttccac tacttcttca tcaaatgttt
    14521 cacacacaac tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg
    14581 catgaaatcc tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc
    14641 cttcttgctt aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt
    14701 cttcgataga attttgccaa tttttaatcg aaaccataat taacctcctt aaactaatat
    14761 gttagttttg atacatttta tattttaaaa cccgttgtaa ccgttgtcaa tataaaaatg
    14821 gaataaactt gctttttttc taccatatca gaattattgt attttttatt cagaaaaata
    14881 aaatactcct tctttttgga ctgattttat cgaaacttat ttttttcttt ttttcatgta
    14941 ttcttttatt tttataacta gtgtattact tcaaaatttg atattatatg gcatgaacag
    15001 aattaaaata tcagctaaaa taaaagttaa ggagcattga gtatgacaat taaaactagt
    15061 ctccaaaatc aagcttacca atctattcgc cgacaaatta tctatgctga cttagaacct
    15121 ggaaaaaaag tatcagaaaa aaaattagaa gaaacacttt ctattggtcg tacaccaata
    15181 cgtgaagcac tgattcaact ccgacaacat ggtcttgttg atacaattcc tcaaagcgga
    15241 aattatatct cattaattga tttagattcg gccaaaaatg ctcgatttgt cagagaacat
    15301 ttggaacgac aaattatgtt agagtgttgt gccaaaatta ataaccaaaa aaaacggatt
    15361 ttagaaacta taatttcaga acaagaaaaa gctgttagtc aaaaaaatgc tcgtagtttt
    15421 tttcataatg ataatttatt ccataaagct tgttttgaaa ttgcagagcg agaagaagta
    15481 tggcaatggt tagaaaataa caatacacac ttagaacgat ttcgttggct acgagtaaaa
    15541 acggaaggat taaagtggga aaaaattatg gaacaacatt atcaactatt tgaggcatta
    15601 acaaaacaag atccagaaga agctaacttt ctaacatcgt tgcatcttca tttaatgctt
    15661 aatgagcaaa atattgtttt agctactcat ccaaaatatt tcaaaagtat ataactttta
    15721 actcttttta aattaaatga ttgttctgat cagaaaaata tcgcttgttt tcttattatg
    15781 taatatattt cataaactat tctgcataac aactattgtt tgtacttttt acattggata
    15841 actaattatg actgctgata acttttgata ttaaactagt atctccaatc caaataaaaa
    15901 atagatatct tgttcatcaa aattagttca tggatactta ccttcattta catcattcag
    15961 cggtttctca ttttctttta ttctattatt tcattttcta atagacttgg cttcatttcg
    16021 caatactttt ttacaatagg catcaaattg gtgttgttta gatatttagt tgtagttttg
    16081 tgacatgata agtatgctcc tttttgtctt ttccactaat acaacgatca aagggtgctt
    16141 ttttcaaaaa actagaaaaa tcaaaataag attcgtattt tgattctata tatcctgttt
    16201 caactacatc ttatatttca taatagaata aatagccttt tgcatagctg cttaacttat
    16261 aagaaattgc aatttacttc ttcaaagtga aaacgaatat gagtaaaata aaattattct
    16321 tcttacagat aattaaaaat agcgcgtaac aaaaatcggc attcaatcac ataattagga
    16381 ttgatgccga ttacttatat tctacgtata gttttagtat tcttttttta tcaccagtaa
    16441 ccaattgaga acagtcctaa agcagaatag tcttttatct caaaacgctt aatttcaggc
    16501 ttaggaatat gaataatctt ttgagtatat tctttctctt ttaatcttac tgaagaaata
    16561 ccactgccta tcttttttac aaatatttca tttggtgtac agactaataa aacaatattt
    16621 gtaatcaatc cttgcactaa agaattaaaa ggaagactat ttaaaacagg gtgtgcataa
    16681 ctataacatg tgaagtagtt attttgaact ttaactttta aaaactttaa aaataatctt
    16741 atttgttctt ctaaacaatc atctatactc atataatcac tccactaatt acttccccca
    16801 acaagaaata gcattaatag ctataccagc tgcaatagtt gcagcattga attttgcaaa
    16861 ttgtgcagga gttaaacttc tttttgctac atctaaaagt attttaacag cataatcata
    16921 atttttccct tgcaacgcat taattaaagt ttgcattaga ttttcacgat taagtatatc
    16981 ccaaacaaca caccaaatgg taatgagttc ataacaacac atgcagcata cttttgaaga
    17041 tcacgttttt gacgctttcc tgttggctgc acaaaatttt ctaaaataaa tttccctgct
    17101 ataccttcag agccatctaa ttcagctttt ttctgtatag cttctaaatc agtaactata
    17161 tattgatgtg tttctgtatc aacataacca gcttcttcta aatagaattt caaactctct
    17221 actgcctgtt gtctttgtgt ttctatatca ggaacttcat tcactttttc agcagcataa
    17281 acactggttc cggcaccgac taacgtactt aatccaatta acattacacc taataatgaa
    17341 aaaaacttaa aaaaacgact catttttatc cctcctgaaa agtattgttg attatcaatt
    17401 tttatccttt taagctgttc aataagacgt ttttacgttc cttccgtttc tattttattc
    17461 tttctgtctt ataacagctt ttttcaagta aagaacgata taattcttct taaaaaacag
    17521 tctatttttt cggtatataa taaaattgta ttgttaccta catagcgaaa cagtaccaac
    17581 atcattcaaa tgaagatagc aaatttgctt cttccttttt cttaagtgac tatttaaata
    17641 accagaaaaa aactaaaatc acactgatat cccaaataaa ttgaaataaa ggaaaaagtt
    17701 tttttctacc atgatagttt ttcaaaaaat agaaactcgt actatttaaa agtaaaataa
    17761 ctagcatcgt aaagactact gatttatcta aacttctata aaaaagtggg ctaaataata
    17821 aagtaattaa aaaatgaact atttgatatt gttttttttc atattaacag atcgcagctc
    17881 ctgcgattcc ataggctgtt cctacagcaa atggaataac ggctgcacct actcccgtaa
    17941 ggcttaaacc tagagcagca gctgtataaa tcccagaatg tagaagtcca gccccagtga
    18001 aagcccaact gcaggcactt cgttcaccag ttttattatt tgttttttga tctttcatat
    18061 aattctcaac ttcatgaagc tgatcggtta gccatccatg gccatttttt ttgaatagaa
    18121 cgattaaatt catttagata ctgttggatt tcagaaactg taatttcaga attaattcta
    18181 tctaaacttg attttaaatc actctcatca ataatatata ctaatcgatt tcctttagaa
    18241 tctttatgtg tggctaaatt attttgaatg atatttattt cagtttcttg ttgttttact
    18301 aaaaaattat ttgtttcaga agcatcgaca cttattccag cgctaacaaa agtacttaaa
    18361 cctattaaga aaacacctaa aataccaaat attttaacta ttcttttcat aatttttcct
    18421 acttaaaaat aagttatttt taaaatatct tgattatgaa ttcatccccc aaaggccttt
    18481 taagattgat acagcagtcc gttcacttct tttagacttg tcatataagt cttttaaatg
    18541 atcccatacc agactagttt gtctatctag cttcaatcca ttaaacgcta atgaacggtt
    18601 cattttttct attactttag ataaagctac agaaattttt ataccttcac aacgcgcatc
    18661 ttggatttca ataatagtat catcaattag tttttgaatt tctttcctat caacatattc
    18721 aatcttagaa agagaatctt ttaaagctac taatgattca acgatttctt ttttctcttc
    18781 agacattctc ttcctccttc tagtgacccg taaagccatg tagagcagag tccattgaac
    18841 cagtaattac actatctaca acgccattcc aatttactct acattttcca tttttcagat
    18901 ttgtcggtcc atacggatag actttttgac attcaatgtt cggattatac cagataggat
    18961 tataccattc agccgcagaa gcagattcag tcgatacaaa caatcccaca ccaacaaaac
    19021 caatacacat cacactatag ccaattaatt ttttcatctc gtatccatct cctaaaaaat
    19081 agaatagttc ttgtagttat tcataagaca tattttttcc gtttttgttt tatcctttct
    19141 gtcctataac aacttttttc aagtaaagaa tgacatactt cttcttaaaa aaacagtctg
    19201 tttttttcta ttttttcggt gcataataaa tatgagtgtt cattttttat tttaatacac
    19261 acttcgtaca taataaatct tgatcgcatc aatcttttga gcttcttgat ctactttcaa
    19321 ttgaagtaat acaaattgag tgacctcatt cgttgtttct tctccaaata acaaatattg
    19381 atccggattc tctatatctt gtgtcacctg gtgaagtttc ccagttgttt tcatttgtac
    19441 atgcgggtct acatcaatac tattgtcttt aatacaacga tccgtcatat agtccctcac
    19501 agcttgattg cgtttataaa catctgtgaa attactccat tttctagcaa aatcttttaa
    19561 gaaatttttt gtcatttcat ctatttgttg cgctgtctcg tcttctaatg aaccaatttg
    19621 actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt cgattgtttc
    19681 ttctgtcgtt gtcaattgtg ttttttgttt ttcagaagaa aaccaatgac aaccagtaag
    19741 acctatcaaa cataaccaac taaatactaa aattattcct tttttcacgc ttctagacac
    19801 tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt gtacgtgcgt
    19861 tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga gaaatgttgt
    19921 ccgccatctg aaacatctgt tataattccg gtgtgaccat agattccagg agacaatgca
    19981 ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt tgggttcatg
    20041 atcacttgcc aaccataggc tgtccaatca taatcttccc ctattaacat cgcaaacatg
    20101 tgtccagaac ccattaactg tgggccacct agttgggata cataccacgc cgtgccacca
    20161 taacactctc cattatttac cgatcgtcca atgatccgat ctaaaacttt cgcattacct
    20221 ttacttttcc ctgcaacaag tgacgatttt tttgttggta ggctattcca atgtttgatc
    20281 gcaaaagtat catattctgt taagttataa gactttatga tactcatcgc tccttggtaa
    20341 tacggactgc cttcggtaat acctgccgcc gcatagcctc cacgacctag aatcaataaa
    20401 caactcccag gatctttatt gcccacacat tgattaaagt tgctttgact cgccaccaaa
    20461 aagccgtaat agtcataaca atctttcagg ttcggaaact tcatatagaa gccaccttca
    20521 gaaccaccaa tcccccgtgc agaaccttgt ggatagggac aaccaggaaa ccaggtaatc
    20581 ccaaagaagt ttgtatcgtt tcttccagat tgtgattgtc cataagtcga ttcataacct
    20641 aattgagaaa gaatcacact tggtaataac tgatacttta ccgctgcacc gactgctgct
    20701 tgcgcatgtt catctgtgat cggaccacgt cctttaatct ctgaaaaaat cggtaatcct
    20761 tcggtatatt cccaattaaa agcgacagct agacctgtag cctctcctcc atcctcggaa
    20821 agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa aagcagacaa
    20881 cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt tttgaactgt
    20941 tcgtcctttt ggtaaagtaa aatcggttcc tggttttgcg tccaaaatcg gcaatgtttc
    21001 atttttcttt tctatttttt tcgattgctc tactggatta ttaaaagaag aaaatgcagg
    21061 ttgtaaggat tttggtacgg aaatcgacgg tttactttca gacgaacgtt gtatctgtcg
    21121 ttgcacagaa cgatttcctg atagagtggc taccgacgac ttaggaacag agtattgatt
    21181 caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca cttggtgaat
    21241 ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc cagcaggcaa
    21301 cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc tttgattatt
    21361 cccttgttgc ttactcgatt gtgccaacga ttgtacctgt tgtttcggtc ttactgaatt
    21421 catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg tttttgattt
    21481 ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac tataaggcaa
    21541 gtcttgattt gaacgcccac ctagttcaaa gggattaatc acttgccccc cttttaccga
    21601 ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg cagaagcgag
    21661 tggattcgtt gctcggtaca cattccctgc taatcctgca cctttgattg ccaaattttg
    21721 cccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat gacttgctat
    21781 gtttttcgtg ttatttatgg tttgatctgc agctcctaac cctagtttct ttaataaatc
    21841 tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat ttcctaaaaa
    21901 accaaaaaca gtattcatta gaattaaatc gtctactatg ttatagatca aaaatgccac
    21961 caaaataatc acactgtaaa aagatttttg aaacatcaag ctcaaaaagg actttaaggt
    22021 attcatgatc atataatcaa atgctggaac aaaagaagcg agtaatgaaa aaggaatcac
    22081 aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa tcactacaag
    22141 tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg ctttataaac
    22201 agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat cttctcctgc
    22261 agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt ccttggctag
    22321 aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg tcccataatt
    22381 taacaataga tacggatcaa caacagccgc tttataataa agttggcgca ctttttgaat
    22441 ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg ccaattcatc
    22501 tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat catttaaccc
    22561 tttagtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc aaaccattga
    22621 aaaaatgaca acaaagagaa atcgaataaa caccattttt gcgtaatgtt tccctctaaa
    22681 tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac caataggatc
    22741 gtatattttc gaataaatag tatccgaaaa agtgaaaaac tggtgaatta actcgttcat
    22801 tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa tcaatttact
    22861 taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg ctccaaataa
    22921 cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat caatatgaaa
    22981 tttcccgatc tcatagatcg attgatccac ctgtttttct accgcatata ccaatccacc
    23041 aacaaaaaac agtccgaatc caaccgttaa aaaaagccaa acgcaccatt tcttttcctt
    23101 acgctttata taagccattg tttcacctcg tttctattgc atgattaacg tatcctgttc
    23161 ttctatttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca tttcttcaaa
    23221 caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc aacactgtcc
    23281 tttaatcata tggctcattt cagataagtt cgcttcattt acttccaaac gtaaatgagt
    23341 caaaatcttt tctcgctcac tgtcttcatc aaaagcgaag acaacaccaa aattcccttt
    23401 ttctttatct gaacctaagt cgccaataaa ttgtgtatct agcactaatt gattcatctg
    23461 tgaacgcccc acacgcgcca ttgctgcaat aatactacgt cccgcgttcg aattattaaa
    23521 tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt ttccaaacat
    23581 ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg catctgtata
    23641 tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta aaatcgttgt
    23701 tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac gtaaaatcga
    23761 atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt gttccatttg
    23821 ttctaaatac tcgaccacat gcatcaagcc aacttgttgt ccggcttctt tttgaactaa
    23881 aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac ctaacggact
    23941 taactcatca atcatatcct gggctacttg tttcgcatct actcgggata aacaaacaat
    24001 tggatctaat acgccataat tacggggatt tgtagcatct aacgtgacat aactcatttt
    24061 ttgcaaatgt tccacaaaca aagggtaatg tttctgataa tacgggtctt gaatgagttt
    24121 cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta cattcataaa
    24181 cgaactatac aaaaagagca atccaactaa aaaggtcttc cctttaccgg tttttccagt
    24241 tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa tagctagaag
    24301 attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg ttttttctcc
    24361 tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg attccatacc
    24421 taattctgat actgctgaaa acaaccattc gccaattgct tctacagttg ttcggtgctt
    24481 ccaatgcaac tgtcgaccca atggttgccc taataaacat ttatagaaca attctcgttg
    24541 atcatttttt gcacggtgga tatggatagt cgtatgaagc tgttgaataa tcgttgcact
    24601 attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga tccaatcgac
    24661 gacaggttgg ccattatcta ctcgttgctt taaatgtttt actaatcccc gaacagttaa
    24721 taagcgttcg ctaggttctt catcgttttg aaccaattcc ttatattctg ttttgaaccg
    24781 ttccgatagc caatttagtt ttaaaccaat acctccctgt cctttcaagc gtggatagtg
    24841 aattttcatt cgtaattctg ctgggaaaga aagatcctgt gcaaattgaa atagatcaca
    24901 gtttctcata ttttcaggtg tttctccaat aggtaacaac gctaaataac tagtgcccaa
    24961 ctcatcttct aattgaagaa ggcctttatg ttccttcggc atacgtacat acgttccttg
    25021 attgtctttc agtacttccc aattattttg ttcttctact ttatgagcca ttcctcgaat
    25081 atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa tagaaaagct
    25141 ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca aagtttctaa
    25201 atcatctact tctaatccaa ataagcgtgc taaacttcct gctacatcta accagattag
    25261 atttagtaca tcataaaaac tctccacggt tgctttttct ttgactttga ttcctaaaat
    25321 aaaggaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt cttttgcata
    25381 atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt caaatttcgt
    25441 ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat ctgctaaaag
    25501 taacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg tgttttttga
    25561 aatggttcgt ggaaaaacat gatagtaggc ccagatacaa tcctcgccat ttaacaaaca
    25621 atttttcttt aatgctttca ctggttcttt tacaagtacc aatagatctc acctcccaat
    25681 taaaaaaagg aacaaccagt tgattgtccc tttttctaat cctctaaata aacatactta
    25741 ttcgcaaaaa gtaattcttc caatgcatta tttgatacct tgtcccagtt ataatacaca
    25801 caatcaccat tcatcatacg cactacataa caaaaccaat cccataaata aaaatgcact
    25861 ttttttccat caggttttag ttttgccagt gctaacacac ataaataggg tagccctata
    25921 aaaaaaacaa cccctgctgg tggaaaaaaa gaaataaccc aaccaataaa tgaacgtaaa
    25981 aataaatatt caaagattac gaccaataaa aaagttgctg cattgactaa tgcaactgga
    26041 aatggaagac gccaattgct tgtaatctgt tgtacgcgat aaggctccgc ataaacaatc
    26101 gaataatcgt aaggttccca atccacttca ctatcctcct aatccataaa taatttttca
    26161 atgattttac caaacgcatc aaatacacgc gttggatcat ttaagaagaa atagactgcg
    26221 gcacctgctg cgatagatat taatgcattt cctacttttg atttcataaa atttcgcaat
    26281 cccagataaa caaccaccat tattaaaaaa tagccacctt gcgttaatat aaaatctaca
    26341 attcccttca gtgtcatttc cgttcgtctc ctttcctatc caaccaatta tgtgtgagtt
    26401 tttcaacata atactgattt gtttttttcg taataacaag cgtcatttgt tctttttgta
    26461 aaagtgacgt atcaccatct cgaaagacga ctgttgcttt tacaataaaa gtttttttcg
    26521 tttctttata acgcaatact ttcatctcta cttgatcaac tttagctgtc tgattgagtc
    26581 cttcaggttc tttcatcatg tatgacatat cttttgcatt gtttttcgca tacttatcta
    26641 agaaatcttc cacaaatcct tccacatctt tcgcttccga cgcatcaacc gtttcgtata
    26701 atgtgtcatc ataagtaatc attcgttgtt tttctgtatt ctttggtagt gccgtaaaat
    26761 aaggataagc aataatacga cagcccttgt ttgaaataga aaagggaaga ttgagtactt
    26821 gtgtgattgt cacacttgtt tctttcttat tctctggatc catcgagcga atatcataag
    26881 ttactttata actagctgtt ggaatggttt caatttcttt taatccaata aacgaagcag
    26941 cagttaattt tcgaatacct gaaacacttc ctggttcttg aataaagttt tgtgcataaa
    27001 actctttttt taatatttct tgacgttgtt gttcttgttc atgattatcg aatgtattca
    27061 tatacaaagc aacaaaacga ttcataaagg attcaatttc aggtgtgtac gttaattctg
    27121 actgactttg tacttctttc ttttgtaatt gttggatttc atctttcaaa aacgacactt
    27181 gaaccttcgt tttcatggat tgaaaaaatg ccaagccccc tgaaaaacaa agaaataaaa
    27241 ccaaacacca aatccatttc actgcttttt ttgaatggat tttcctttga ttcggtactt
    27301 tctctgtata aactttttct ttccgccgta gttgtcgttt tttcataagc tactccttct
    27361 agtatgtttt tactagtacg tacgattgac aacgataagc ttgtttttct gaaaaatagt
    27421 ccattcaatt accctcattt tctattcctt ttctctttct tttatatcca acaaatccag
    27481 caatacttgt caacactcct cctagaacag aaagtacaac atgtatttct tcaccagtct
    27541 ttggtaagac ttttgttttt ccatctgctg ttttgacttc aatattgcca cttggaagtt
    27601 tctttacagg aatcgacaaa tcctttacca aattcccttc actatcttgg attaatggaa
    27661 ttccatcttt tacaccaaca attgtttgtc caccttctgc ttttagtgga gttgttggtg
    27721 ttggaatatg ggtcaattcc tctacattac tagtgacaat cggtacactc attccttgag
    27781 aattattcgt cgtaatttca ccgtttgggg ttaccgatac tgtgacttct tttgctggaa
    27841 caataggttt attttctttt ggaattgctt ctgatttttc agcaggttgt ttcggttctt
    27901 cttttggttc ttcactggta aacgacatcg ttggctgttc cgtacttgac gaatcggtat
    27961 tcgtttcaga agaactggtt gttcctgatt gttctggagt tgtgactata cttgaatccg
    28021 ttgttgttga actgtgcaca ccttcttctt caattggatc agtcggcaaa gttgattcag
    28081 gaatctctgg ttctggtgcc actggctctt tttcaggttt ctctggctgc aaatgttcag
    28141 gttctgctgg tttttccgtt gttcctccat ttagctctgt ctcaacatct gataatcctt
    28201 cccctggcaa tgtttctaca atttctgact taggtgtttc tacagtttca tctgcataaa
    28261 ccaatgaact ccctaattga aacgaaaata atgaaattcc tagtgttgtt acgactgcta
    28321 atgttgtttt tttcatgatt attcctccaa ttttttatgc aattcgtgtt ttgttcatag
    28381 acgccgcact aatattatct gaacgataaa agaactcccg tccgttttct tctaacccaa
    28441 catatggatt atttttgaat aattgaatca atagttcact ggtgttgtat tttggatagt
    28501 ttgcaaaaaa ataatactgt gcttgtcgcc catccgaaaa ttcaacaagt aattcataac
    28561 gataccgctt caatcctttt cgttgtttta aaggatctct ataccaactt gtccatcgtt
    28621 ccgttcgtgg accgagatag acacgataca catagaacaa tgccatacca cataccacca
    28681 aaaataataa tcgccacata aaaattcccc cttttcttct acctttcttg aatcaccgaa
    28741 cattcatacc ctcggtagag atccgaaaaa tccatcatta atttttcgta taaacgttgt
    28801 tgcaaatagc gattgtttcg aatacataat tcttcatagc catcaatcac ttgatcttct
    28861 ttctcatagc atcttgtagc tctatgacgc tcagcttgca tgacataacc aaactctgca
    28921 ccatatcgtt ctaccgttaa taactgttga ctacgcaata atgcaatata acaatcacaa
    28981 aaatgttcag ccgacatttg atttttctca cgtttgtgaa ataggtattc tttagaaagc
    29041 acattattcg ttaaacaatc tgataaatag cgataatctg gcggtcggtt attttcatct
    29101 gtgaattttt cccataataa actgattttt attcgttcag cggctagata gtcttgttcg
    29161 cgtggataaa attcatcaat cgtacaaagc ttttttatca cccgaataaa caaatccata
    29221 taatgcgaaa cgaactcttc cttttcaatt ggctctccta aaaattccag tgacatacca
    29281 tcttctcctt ccttaaaaaa taaaaaagca gaaaccgatt tggcttctac ttttctttcc
    29341 ttttatagct cttttttacg gaagaatccg caaatgacat tttttcaaaa aattaatcgg
    29401 cacaagttca cgaaaacttg gagcaatttt agatagaatc acttccccat cttgtaaaat
    29461 acaatcataa aaatcatcag ttacttgata cttacgcttc ttacaataat tgataaaata
    29521 gtagcgatca ttttctattt ccattcgttg ttctactaag cgcatacgaa catcatcata
    29581 gaaataagcc acaaatcgcc cttcaagttc acgtttttca atgggatact cttttggtaa
    29641 caatctattt tttcgtcgtt tatacattgc tggatacctt agaagaagtt catccaaacg
    29701 tctgtcagta tatggcaact gacgaatcaa tgattccaat cgttctttat acagatagaa
    29761 taattggcgc aagcaatagt gtactgcttc tggattttct aatactgttg gatcacgcga
    29821 ccaatacgca ccaacataaa tcataatttt tttcatttag acaatcacac cagcaatact
    29881 aatcaccgat ccacaatagg tagaacaagt ggcatagcgg ccgatttcct ctaattccag
    29941 ctcaatctct tctaaagaaa gatacgtctg ttcttctgtt ggctggcgat tcatttccca
    30001 agtaacagtt gccaacgaga tttcttccac aagaggtttt tcgttgctaa gtgctgccac
    30061 acagttttcg tatatcttca ttttatcgca gccttttttt accgctaaaa tcgcaggtac
    30121 cggcaatgaa aatcgataaa agcgaatgaa ttttagcgtt ccttttcttc ggctaaattc
    30181 caactcttcg agttcttgtt cgtacccctt acgaacttct aataagcgtg caatttcttg
    30241 ccagttttct ttttcctgat aacgtccagc ctttaccgct tgttcaatat aaggaactcg
    30301 aaaacgaaaa taagacaaat caaccatctt ttcatttaat gcttcaattg cagcctttaa
    30361 atccaaaaga cgcagccatt ctgttgtcgt atatgcattc atgcaatttt ccctcctgac
    30421 gttcccatcc attctagata gctttgttgc tcgtcattta gttcagcatt ctcaattcct
    30481 tcttgtacaa agtctcttcc taaaatacga tctgcttctt gtaataactt taatgtcgga
    30541 gctacttgtt tcaaaaacca gcgttctgaa cgatctaatg agaatttttc tggctttacg
    30601 gtaaaatcca attcttccat cgaacgaaac aattttgccc atcgttcatc aacaattcgt
    30661 gcatcacttt ccagtcgatc ccaaacaata agttttgcat taattaaacc aacgccaatc
    30721 tctcctaaac gtttagggga atgatccaaa ataaacgctt ctactgcttg tgttgctttt
    30781 tcattagcta ggcgtaactc atatcgatta taaatgccat atttcgttaa tgcttctatt
    30841 tctgaaattt tctcacgatt cgcaatttca aaacgttttt cgtagaaatt gaaaaacaat
    30901 ggactctttc gcgatccaaa atacaaggat aacccttcgc ttcggaaata accatcatct
    30961 aaaaaacaac cgccaatatt ctgaaatttc ttaaagtcca tttctaataa acctgctttt
    31021 tgtttttcta gtaattcaaa gagatcaaaa tgttcttctg tttccaatcc ccaaagttca
    31081 tctaacgcaa tatcgaatcg actacattgt actcgtttat aaccagcttc caactttcct
    31141 tgatttttcc aaatggaata tagccatgtt cgccaatctg ttcgttgttc acgcatgact
    31201 tcctcatatt ctcggcaacc ttgacccgaa aactgtacaa gtacattttg tgttacagtt
    31261 tgttttgaat gccagaatac ccagatattt ccacgaacag aaagacaatc aaaaagatac
    31321 aagcccgtcg gacgtgtttc aaattctgtt agatccattc gtaacacacg ttgaatcact
    31381 attttgtagt ctgtcccaaa aaaggagaga cgcaaatagt cgatcattgc tcgaagatct
    31441 attccgcttt tcatatacaa tcgaaagcgt tccttgattc gtttttcgaa atcctctggg
    31501 attttctttt ttccttgttc catcttcttt atataatcta ccgagcatcc caataactgt
    31561 gcaaaacgtt tttgagagag taattttttc cctccacctg aagcgtgaat agcactcaat
    31621 ttttttcgat aatttcgcaa ttcaataggt gtcatattct acctccttta taaaataaca
    31681 aatttcgatt ttcttaatct gctagaaatc ccgatataac aggtggtacc ccctaatttt
    31741 tctaaaaagg agtactcctt ctaaccttag ctaaagcctt gatataactg atttaaatac
    31801 acttttttat ttgtgttatg ttatttctgc cccccatatt atatatcggg gggctttaca
    31861 caaagaggcc gtgcgcaagc gcacgacctc tttgtgggca cacatgccaa gcatgtgctg
    31921 ccacgactgg tctcggtggc ttcgccgaca caagccgcgg cagcacatac tcggacaatg
    31981 tgttctccca tagtccctat gaaagccacc ccgatatcta aaccaatgaa tcttcttttt
    32041 cttgtaattc tttatactgt tcaggagcaa aacactgtaa aatcgctaaa cgtaactgta
    32101 tgtttttctc tttgcataac cgatatacct gacgaaaacg caatatatcc tcttttgtca
    32161 aaacaatctg atttctcgta acaaacaatg tctcgcgctc aattcgtcgg aaaacataag
    32221 aaccttttgt gtagtattct actttgttaa tccactcgta taatcttcga accgcaaaat
    32281 caggtgaaat ttcacccgca gctgaagaaa cacgatacaa aacttcaccc atacttcacc
    32341 acttccttct aaattataaa agtgtataac tttcctcatc taccccattt gttttcgtat
    32401 ttccttttaa cgattgcgct tgacgatcaa tttgttcttc gtaactagta aacgcctctt
    32461 ctttcagtac agaaaaatct tgcacaatca ttgtgtcaaa ttttttaaat tcctcaataa
    32521 agtgatagtc tttgtaaaca aatggcgcaa agaattctac aggcacatca aaaccaggaa
    32581 aagcataccc acgaccaggt tgatctttgt tataaaacgg cttattctta aaatcctctc
    32641 caaaagtcat taaatagcca atgggagata aacggccaac agaaatacgg aacgttaaac
    32701 tatctcgaac accacttgga aaatattccg catctggtcg ctgcatcgct aaaataacat
    32761 acatgcctgc ttgacgacct tttaatacta atggctgaat taatttatca actttgtttc
    32821 tcgtttccat atccaacgaa ccatagaaag ctgcccattc atcaaaaaag ataaaatgcg
    32881 gcggtatccc ataataagca taatttttcc ctggttcata cttaggctct ttcatcgttt
    32941 tcatataggt atatcgctga ttcatttctt gtaacgcatt tcgtaagcaa ataatcattt
    33001 cttcattcgt tttgtaaaac acatgtcctt tgaatacatc aatatcacct aatgccgcta
    33061 aatctgccgc tttcccatcg caaatatcta ctgtccctac atctaaacaa gctttgatca
    33121 atgaatacaa taaataggtt tttccgccac caatcgcacc agaaattaat agatttggcg
    33181 attgatcata tacccaatcc aatgttttca ttaaatgaat acgtccattt tctgctatac
    33241 attgatcaat aggaatccga ccagaatcgg aaagaaatac ataattgatt cgggcttttt
    33301 ctcgttcttc acgaattaaa tcacgcaaaa atacttcgga taataagcta cccattttta
    33361 aaaatcgttc gtggaaacgg ccaccatcta aaggaaaaga aaccactaat tcttgtttct
    33421 tttgataaaa ataaactttt ggtaatctca ttttttcctt cgtttgtttc tcaccaactt
    33481 gcttctttgt ataaacaaac cgattgtcaa ttaacattcg agcaagtaac tgacgccaat
    33541 aaacagaagc aaaatatcct tcctttagtc gcgaccaacg catcaaacct aaaattaatc
    33601 cactaaccag caacaaatac atcacaatac tgattccagg aataagatac atttgccaat
    33661 tcgaatacgt atttaaacga ggataaaata ctttccaact aaaccaactt aatagaatca
    33721 aaaatggtaa cagaaagaaa ccataataac aaaaccataa ataacgattg aaataacgaa
    33781 ttcgtcgacc acgatactta aaaaaatgag gttccaattg tcacattcct cctttctgtt
    33841 tttacttctc ctgtgctaaa aatttttgac aaaaagcaac taatactgga atatcacatt
    33901 taataaaaaa ccataatcga aaaagattta atcgattttt atatgggtgt gcacacatat
    33961 tacatattcg tactacacga atccaaacca cttcctgatg gtcttgtttc cactgatacg
    34021 gtaatgaaaa acgaacaaga tcacgtaaaa acatacactg cccaataacc gcacaaatat
    34081 actcatttgt ttgtataaaa atcgttggat tgtctccaaa atactcaatc aattgttgga
    34141 tattttgaca acaaaccata atctctcgaa gtgtttgatt ggttttgtcc agattcaaaa
    34201 tctccccttc cttctacctt aaatagaaag agtaggaaga cttcctccta ctctttaaaa
    34261 aacttatttt ttctcttttt ccttagaatc agcacttggt tgtttttgat ctaaaccttt
    34321 ttcttgtgat tgtttcggat tagaattatt tgttggagct tctactttct ctaatccttt
    34381 cgcataaatc actaaatctg aatattcacg attgccatct tgtgctggaa tatataccac
    34441 atgatattct ggatcaatta atttcacagg atcacgtcgt ttaaaaccag tcaaatctac
    34501 atctgctgga aatttaactc gtcgtgcaat ctttggttca tctggatttc caaaaactaa
    34561 ttgtgctcgc tgtgatcgaa tttctcccgt ataatcgcct gtttcattat caaatacacg
    34621 atcggactct tctaatgcgc caaagcgtac ggctcctata caagtttcat ctaatgcttc
    34681 tgtaatatca taattatttg gaaattttaa tcccatctta caaaacctcc tcaattttta
    34741 caccagttaa aaagacaccg acatcttgat agccagttgt tcgacgctct gtttgtccca
    34801 tatcatttgt ttcacgacta ttcactcttt ctttacgaaa aacaaaataa gcttcttcaa
    34861 atgaaatacg tacctctttt tctggtaatc gtaatctttt atcctcttca gctgtgagat
    34921 aaacaaaaaa agcaggctgt tcttctcgcg aacattctgc ttgtaattga acatatcgcc
    34981 cagacacacc attaatttcc tttggtggat taggttcccg tgaacccaat aaaaatacct
    35041 gacctaaaaa ctctttcttc atacgcaatg ctttttctgc catatcattt ttcctcctct
    35101 taaaattcac aatcaattct aaacgactct atattacaca atggaaagta atgtttaaca
    35161 acaaatggct catcactagg agacatatct actattaaca tcccgtctcg ttcatgcaca
    35221 gaaatatcag tacactcctc atacgtacat tcttttccac tcaaaaaaca aatatttata
    35281 tttttctttt ccatattctt ttttcctcca ttattttctt ttctgtaaaa accaagtaat
    35341 aattcctttt ccatcttcca acacaaactg atgatatatt tccctagcaa gaatccacac
    35401 acatgctaaa ataaagaaaa taataatact actaaagatg cactcatcct tgctgtcaaa
    35461 cggaaaaaga tatatcataa ttgcctcccc cataattaag aaaccaattg atacaaacaa
    35521 aaactctacc atcatacaaa agaatatttc ttccaactct tttacatcct ttctaacttt
    35581 ttataaaaaa ggagattatt tttataaaca cttttattca acaactattt tcaaatgaat
    35641 tacacattat catctcaagt atagtttcaa tcgtcactat tgtttggtcc atttctagct
    35701 tttatttaaa acatcgctat acaagaaaaa agaaaagcac tgaaacccta acaaaagaaa
    35761 tgtttattcc atttaaatct tcaattgaaa attatctatt tcaaaaaatt acaagaaaaa
    35821 atattctaga acgaaaacaa acaattcaac atctaatcca aacagttgaa aataaaaata
    35881 tagactttta tttgccttat gaacttattt attttgccaa agaaatacaa aaaataatta
    35941 acacaaataa acattcgaaa aaaatcctct tcaaagtaca aagaatgtat tttgaattct
    36001 catactgcta cctagcagaa ctaaataaag taagaaagca attaggagta gctaaacgtg
    36061 atttcttcta tagaaaaaaa gaaactgtat tatcacataa ttatattcta tctcttttgg
    36121 atccttatat tcttatttat tacactcagt atttcttggt gtatcctcat gatcatttac
    36181 tactcaacac aataatttat ttgcctttga taaaaaattc tttctaaaaa ctaaacaagc
    36241 ggctagttgc ctaaccgctt gttcgtcctc tttagtctct gactttatac aaatcataac
    36301 caatcaatcc tgccgcaagg acaaagatca taccaatcat gatccataat acattgtctt
    36361 cagagcctgt ttttggcaac ataggtacca ttgcagcttg tttcacttct tttacaggtt
    36421 gtggttcgtt tggttttact ggttgcggtg gttttggtgc ctccacaaat tgaaccgttt
    36481 gatctttgtt tgttagatcg acgttatgtt ttgcgacttc ttcaaacgtt tctttttcat
    36541 cttctgtttt ggcttggagc cattccgcaa aactcaatcc ttttcctgca aggttgcgac
    36601 cgtcaacgat gaattcaaac aagaattcca tcatttcttt atcagccata aagacttttt
    36661 ccgcatgtgt tccttcaaat ggggtgccgt ctggtgttaa gtagccttga acaatcagtt
    36721 tgtaaatttg accgattgct aacccattaa cttgaaccca ttcgacaacc ttttgatttg
    36781 gtaaagctaa gatctctttt ccaccatttg cattggtagc ttttgtcgta atggttggac
    36841 tgacgatttt gatggtttgg tctttgtcgg taatatctgc gtgagaagcc aataatacgc
    36901 cttctctaaa cagatcttca aaaactacga cttctttgct tcgcaacgca tgtgcgtcta
    36961 ggataaagtc tagattgact gtaccatcag ttgtttctgc cacaaacgtt aaggtacttg
    37021 agacttcttt tccatctact agcactggtt tttccgttac tttgtccatt agaacaccgt
    37081 tgactgtgta tgtttttcca acgatcaaat cgtggtattt cacagcgtct gtaatggtga
    37141 ctttcgttaa tggattaacc tctttcaagc cagtgggttt atgtgtagcc gttgtacgta
    37201 cacttgggtt cgttactcgt accgtttgac ctttgtcgtt gatgtcttta tggtttgcta
    37261 gttcttcgtc atttctaaaa agactttcaa acacgaccat ttcacgacca tttaaacctg
    37321 tcaaatcaaa ggtaaatggt acgatcaccg tttcattttc tgctttggca ataaagccaa
    37381 cagcactttc taccggttga ccatgaacaa gtaaggcttc accagttgct ttatccatta
    37441 agattccttt gaccaagtaa gcttttcctg gaaggatctc attaatttca acggtatcat
    37501 ttaaggtgaa cagccccgtt gggtctgcga tcgtcaaccc gtcttcaaat acagctgtgg
    37561 tatgaaccgt tggattgttt acccgaacac tttgattgtc atcattgata tccacatgta
    37621 ccgcaacttc aatgccatca cggaaaagtt tttcaaagac aacgattgtt tgacctttca
    37681 gaacactagc attaaccaca aattcaagat cttttgtgcc attggctttg tctgcaataa
    37741 aggtcaagat acttgtgatt tctttgcctt cttgtaaaat tggcgcattc gttgccttgt
    37801 tcatcaatac acctgaaata gtgtattctt tgccgacaaa caggttggta taagaaacac
    37861 tatctttaat gacgatagtt tctaatggat caaccacttt ttcaccatcg gctttatttg
    37921 acgcttgtgt ttctaatttt ggtggaatca aatagttacg tacagaaacc aataacgttt
    37981 ccccatcatc tggcgcattt tgtctgaatg taatagtgaa tgcttgtttg cctgtatttg
    38041 gttggaaact ttctaatggt acattttcgg taaagtaata gttattggca accagacctt
    38101 ttactaaaat ctcaccattt tgatcggtgg tatatttacc aattgcttga ccttttccgt
    38161 tatatagcgt aaacgtcaca ccggcaagtg gtgttttctt cttgttccat tcttcatact
    38221 tgaacaattt tacacttttc cgtgcttgac ggttcgcaaa atggatcact ttgatcggtt
    38281 ctgtatcatt ggtaatcgag aacactaatg ggttttgatt caaaacgtaa tttacgggtg
    38341 ctttgatttc ttccaaacga tagcgctctt ttccataagt aagtgtaccg ttagttagga
    38401 aaaagccttt ttcattggtt ttaaagatat ccgttgtttc tgtatcattt ggaatctcca
    38461 ttgtgacata acgattggct aaagtatcaa aaatcttgaa ttctgtattg gcaagtggaa
    38521 tcacttttcc tgtttcttga tccactttca ccactttaag acgttgttca attactttat
    38581 cttctaaaac ataatggaaa gtttgcccat tttcagaaat tgtgacttca agtggttcaa
    38641 atagtttata cccttctttt cctttggttt cagaaagaat gtaggtatca aagacaacag
    38701 tatttttacc aaaagtagcg taaccatcat tgtctgtcac aacggttttc accactttgc
    38761 cagttgtttt tgacgtcaaa gagatttcaa tgccagctaa ttttggtttg ttatctggat
    38821 tggttgaatc gactaaatct ttattagcaa ttttcactaa atcaatggtt cctttgatca
    38881 cttgatcttt tgcggtcgca ttgacagttt ttcctggttc aacaacaatt tcaattgttt
    38941 catcagaaag tgcatagcct ttcggcgctt tgatttcttt taaaacatat ttgccaggat
    39001 caagatcttt aatttctgcc tgtactttgc cgttcatatt tttcaaggta atttctttga
    39061 tctttttccc atctgcttga tacaagccat acaccgcatt ttctaaagaa gctgcgcctt
    39121 gtgctttcac accggtttct gcatcttctt taaagacacg aatgtttccc tgtttcaaaa
    39181 tagtcaaatt gacaacagag tcaccaggtt caaaaggatt caatatacca attgtttggg
    39241 cacctggacg acgatacgct aatggcgtac ccaatacttc aatctgattg aaatgaattt
    39301 ttgccttttc tggtgcttct tttgtgactg tgatccatgc ttcattccca tgaatagata
    39361 cattgatccc ttttggcttt tgaatgcctt ttacacgttg tagaaagtca ttgccacttt
    39421 ttacttttaa cgtttcgcca atttttatcg tatgtgtttg attatgaaaa gtagctctta
    39481 catcaaaatt attaagcaga cgttgaattt ctgcttttcg acgatcataa tcaacacgga
    39541 tatttttgat tgggattttt gtgcccaaag cttcccaaat cataaattgt gccgcaatgt
    39601 attgttcatc ggatttgtct ttatttgtga catatcctaa ataagagatc aagcttgttt
    39661 ttttccgtac atttgaattt ttcaaatagt tttccaaagc agtagctgta taaacttgac
    39721 cttctgcggc tgagacacca ggctcaatac aaaacgccac ttgtccatta atctcaaaac
    39781 gaaccatata atttaaagtg aagtttggat tgttcggact agaagtaaca ccaatatttt
    39841 cgccccaatc aattttccga ccacctaaaa cagctgtatt ggttgcacgg cgaacacgaa
    39901 ccgaacgatt ggttggtact gttactaaca gtgtaggtgt acctgttttt ggaatgtcct
    39961 gctctttttt cttttgatct tcaatactag ccgtagattc tttgtttagt ttcgttggtt
    40021 gattttgact agctgcggtt gaagagattg ttttttcaac gttttcctca ttttttcctt
    40081 ctttgtcagc agtagccgct ggttgttcca ctgtttcttc tattcgttgc ttttcttccg
    40141 cttgtttctc agaagactct ggcttcacag aaggcgttgc ttcggcagct ttttctcctt
    40201 ctggagtgga cgtttctgaa gcaggggttg taattgaggc agtttcttca actggttgtg
    40261 aagtaacagg actattccct gggtgttgtg tttccccaat cgtctgatcg agagtcgttt
    40321 tggaattaac cgttggcgct tctgtcacag gggcctgttc aacagtggct tcaccagctg
    40381 ggaccccggc ttctgctggt tggacttctg tcacttcaga agcgacttgg gcaatggctt
    40441 ccaccgtagg ttgtgtagcc gtcgtttctt ctgcatgtgc ttgagcagtt cccaccaaga
    40501 aacagaattg agataaggcc accaatgcga gtgctttcaa ccaattcatt ttcgtttttg
    40561 catacacaag aattcctcct tttcctgaga attttatata ataaaacaaa acaccgttac
    40621 caaaagtgat aacgacgtga actgtctctt ctatttttat ctggcgctct tatctgaaca
    40681 catttcatca atgattttac cctcccacta taattacaga acaaaaaaag aacctgcttc
    40741 gtggaaatca actgtccatt tcccaattgg aataccagct ttattggcaa cttcaccatc
    40801 accaaatatc atgccatacc aattactacc ttcatcctta atttctttcc aaccatatga
    40861 catagcttca tctaacgaat caaaaatcat gccactgttg ccaattgttg caggatctgg
    40921 cttaagatct tctggaatag aaatatcaat cacaccataa tgatccgatt ccccagtatt
    40981 attgtcatta ctagctactg gtttggttgt tctactgact tattttgata caacaagtac
    41041 gcttattctt cttttacttc tccgttttca atttcccatt caaaccactc cgcaaagtct
    41101 tgcttaaaac catctactcg atacctattt tgtaaatagg catcttctga cgtcgtagga
    41161 tctaaatagg taggtttatt ctctggacga gcatagacac ctacccatgt ttcgtaatct
    41221 ccttcaaaat aactatctac caaatagcct ttttttctaa gaatctcccg aacttgattt
    41281 ttaattattg atacttgttc aaaaccatca ctcattctat cacctcaaat aactctagat
    41341 tctcataaat attcccgaca atttcaatcc tatcaatgat ctctaacaaa tcgctactta
    41401 tgcttcccca ttcgtataag aatttccctt ccgcaaaata aattttccat aaacttcttc
    41461 atgatcgtcc caaccaatat cactctcgac aaattccacg ttgttcttat cttttagctc
    41521 agtaaatgcc ataagttcaa catgttttcc aacctcgaaa caatcacacc acccatcaac
    41581 cgttacacgt gtgccttcaa tacctaagaa actttgtcct cgtgtattaa acactaaaac
    41641 tttccgcatt ttttgctcat taattaatca cgcgcgaaat ctaggaatca caacttgttc
    41701 gcttcttctt tataaaataa aaaaactcca ataatcggaa attactatct tttcctcatt
    41761 ccttttttaa atcttcatac ttcttcttat ctttgtccta acctaataac acaaattttg
    41821 aagaaaaact cagtaaaacc tgattctttt tttatcttca caacaaccaa ctttttcttc
    41881 agatcctttt actagaactt gtatacaaaa attgttgaat gctgatacaa tttgcggtta
    41941 cttgaaaaaa cgatagatgt aaaaagataa atagaatcat ttttttattc tatatcagtc
    42001 tatgattcat tgcttatcaa tcacccaacc cattcgatgc cgttttttta agagaaattt
    42061 aaaaaagacc gtacgaatct cgcattgtcc gattactttt ctagaaaaga tccccaattc
    42121 tgtataaata accctatctt cttgttggtt tcattttctc atataagaat tcagtcaatc
    42181 tacccactat gaccattact acgtccaaca caaccgacat attaaccacg taagtattcc
    42241 ctcacagatc tccgaatttc catggtatgg attcccatcc tgacgctgca gaggacaaca
    42301 tatttcatgt ggctctgtac ccttttgatt tggaaagact acaaatcaat actcatctat
    42361 aacttatcaa gcgaactttc gctaggcttc tagtgattac taaattgttg gataacatac
    42421 ctagcccatt ttatctagct ggtattcata acagttattt cttgtattac ttcttccgct
    42481 tgtgttgcca ttgagcttgt caaaaagtta cttagaaaaa agctgccagt aaagcaactt
    42541 tcaacaaacc ctttttgttt cttatcatat caagaaacct ccttttataa atttttctat
    42601 aataaaaaaa cgtcgataac ttaataagta tcgacgtaaa ctatttttat atcccgtaaa
    42661 tcaaaaattc taaagaaaga tttcctatat aagcatattg gataattcga ttagtattgg
    42721 gtatatctct accattctca tacctatcta tggaccattt tgaaattgaa ggagaaaaaa
    42781 gctttccaaa ctcctccctt tctagataat aatctttcct aattttcctc atccttaatc
    42841 ccatactaaa agaatttatc ttgttaaaat cgctaactct ttcatctttt ggaagtatat
    42901 tcttataatc ttctccgtac ataaggtaag gaacagtgac cttaccaaga taggcaatat
    42961 tcattagttt ttcaatatct ggaatattaa tacctttttc ccatctactt ataacatttt
    43021 tgcctgtata gatggtaaag tttttaccaa atttttcaag cgtctcttcc cggttctttc
    43081 ggacttctcg tattcttttt cccataacaa ttttatcgtc ttttgataaa ctaaatatct
    43141 cactatcacg attactctcc ttcgaaattt ctttttcatt acttaataga aaaatatcga
    43201 tttttttttg catttctttt gacggatttc tctttctatt taacacataa ctgagtaata
    43261 cttcactaat tcctatatta tcagctaact ctttgttaag taacttattc atatctctgt
    43321 atgcaattag ttgaccgatg agaatctcaa tcttatcttc ctcatctttt attagagttt
    43381 ctatattcaa aatcttgctt agagtattat ctacgtcttt agggattttt cttgaccctt
    43441 tttcatattt aactaaagta ctttgtgaaa tacctgctaa atcagctaac tttttttgag
    43501 atagtttttt ctcttttctt agagatttaa gaacccttcc acttgaatta tcaaccatga
    43561 aaaaagcacc tcctatgaga tgctttcatc atatactgac agctaaaaat cttcaagaaa
    43621 aaatattcca attttgttaa ttttagtatc gccgtgataa ccaaatgaca gtttaaaatc
    43681 ttttatacag ataatcctca gtgttgcatc tactcaaaaa ggttgattta ataggttttt
    43741 cgattgatta taagttattt cacactatct tgctcatcca ttttcagaac ggccatgaag
    43801 gcttcttgcg gaacttcgac ggagccgatt tgtttcatcc gcttcttccc ttctttttgt
    43861 ttctctaaga gtttacgttt acgagaaaca tctccaccat aacatttagc caagacgttt
    43921 ttacgcaagg ctttgatgtc tgaacgggca acgatttttt gtccaatagc tgcttggatt
    43981 ggcacctcaa attgttggcg tgggattagt tttttcagtt tttcaacgat tgcttttcca
    44041 cgttcatagg caaagtctcg gtgaacgata aagcttaatg catcgacttt ttctccattc
    44101 agcaagatgt ccatcttcac taacttgctt ttttgataac cagacatttc ataatccaat
    44161 gaggcatagc ctttcgtgct tgatttcaat ttgtcgaaaa agtcaaagac aatttcagaa
    44221 agcggaatat tatagacgac atttactcgg taatcatcta aataatccat cgtaatgaat
    44281 tctccccgtt ttcgttgaga aagttccatt actgcaccga caaaatcatt tgggaccatg
    44341 atctgtgctt tgacgaatgg ttcttctaca tcctgaatcg ttacaggttc aggaaaatct
    44401 gccgggttat ctactgtagc agtcgtacca tccgttttat taacatggta aataacggat
    44461 ggtgcagtcg tgatcaattc tagattgaat tcccgttcca agcgttcttg gacgacatcc
    44521 atatgaagaa gtccgaggaa tccacagcgg aacccaaaac ctaatgcttg agaggtttct
    44581 ggttcgaatt gtaaagcagc atcattcaat tgcagttttt ctaaagcttc ccgcaaatcg
    44641 ttgtaacgag aagtatcaat tggataaaga ccacaataaa ccatcggatt catttttcgg
    44701 taacctggta aagcttcagc ggcaggattg tctgctaacg ttactgtatc ccctactcgt
    44761 gtatcttgga cagttttgat acttgctgtg atataaccga catcaccgac catcaaaaag
    44821 tctctagcaa ctgcttttgg cgagaagacc ccgacctctg taacatcaaa tgttttgcca
    44881 ttactcatca gtttgatttt gtctccgggt ttgaccatac cgtccgtgat ccggacattt
    44941 agaacaacac cgcggtaact atcataaata gagtcaaaga tcaaagcttt tagcggtgca
    45001 tctaagtctc cacttggtgc aggaacatat tccacgatct gctcgagaat atcttcaatc
    45061 ccaatccctg atttggcact ggctaataca gcttcactag catcgattcc gattacgtct
    45121 tcgatttctt gacggacacg ttcgggatct gctgcgggta ggtcgatttt attgatgact
    45181 ggcaggatct ccaaatcatt gtctaacgct aaataaacat tggccaatgt ttgtgcttcg
    45241 attccttgtg ctgcatcgac cactaaaacg gcaccctcac aggcagcaag gcttcgagag
    45301 acttcatagg tgaagtcgac gtgccctggg gtgtcaatca agtgaaagat atatgtttct
    45361 ccatttttcg ctgtgtaatt gagttctaca gcatttaatt taatcgtgat tccacgttca
    45421 cgttccaaat ccatcgagtc taacagctga tcttgcattt cgcgagacgt cacagtgtgc
    45481 gtcatttcta aaatacgatc ggccaatgta gatttcccat ggtcgatatg agcgatgatc
    45541 gaaaaattac gaatctgctc ttggcgttct ttcattttct ttatatccat agcgtctcct
    45601 cttttcatat aatcagcact ttccattata ccaacgaaaa gcagtaattt aaagagtttt
    45661 taattagtca gctatagaag ttttttttgt agaattttgc tatactgtta tcaagctaat
    45721 aaatggagat gaaaaaatgg atcaaaaaga ttttagagaa aatcttgaat taaaaccggt
    45781 cactcaagaa catatcgatc aattcaatga attgctttcc tatgtgttcc aagtgactga
    45841 agcagacatc gaggaaagcg ggtttgagaa taaacgagct tttatccgtt ccaaaaaacc
    45901 gattttggaa gtatcgaaag tgttcggatg gttccacgaa aataaactga tctctcaaat
    45961 cgctatctat ccttgtgaag tcaatattca cggtgcacgt tataaaatgg gtggtgtgac
    46021 tggagttgga acttatccag aatatgctaa ccatggattg atgcaagatc taattatcgt
    46081 tgcattggat aatatgcgca agaataagca atggatctct tatttgtatc cttacagtat
    46141 cccctactat cggcgtaaag gatgggaaat catgtctgat aaactttcct ttaaaatcag
    46201 agatacacag ctacctaaac aaatcccagt aagtggtatg gttgagcgat taccagttga
    46261 tcatccagac gtattttccg tttatgacaa atttgcccaa caaaaccatg gtgctttatt
    46321 ccgcagtgat tttcattggg aagagtattg gcgtttcgaa aatgaagatg agcgcacagc
    46381 cgctgtctac tacgatagca accatgaacc aagaggtgtt ctgttctact gggtagctga
    46441 agaaatattt cacgtaaaag aaatgttcta tcttgatcaa gaagctcgaa atggcttatg
    46501 gaactttatt tccgcacatt tttccatgat ctactgggta catggagata tttataaaaa
    46561 tgagccttta gcatttttga ttgaagatag tcagatcaaa gagcagatcg aaccatattt
    46621 tatggctcgg atcgttgatg taaaagaatt ccttcaacgg tttccgtttg ttggaacagc
    46681 tgatgctttt catttcatca ttgaagatcc tgtcgctcct tggaataatg gtgtcttcgc
    46741 tttaacatgg gatgaacagg gacaagtccg tgtcttaaat gaacctatag gaaaatccgt
    46801 tcgactggat atccaaacat tgacttgctt gatgatgaat tatcgccgtg cttcttattt
    46861 ggcaagaatc gaacggttag aaacagatga agaaacactg aagtcactag agcgtatcat
    46921 tccaaacatg gaagcttact ttagcgatta tttttgataa aaaggatgat gtttagatga
    46981 aaatttattt tgccgcacca atgtttgcta aaagtgattt gttatataac caacagttag
    47041 ttaaagaaat cagagagctc tcttctgagc tttcgatcta tcttcctcaa gaaaatgaag
    47101 caatcaatga caaaactgct tatgcagaca gtcgaatgat tgctcttgct gacacagaaa
    47161 aagtgttggc tagtgattta atgattgctt tactagatgg gctgacgatc gatgccgggg
    47221 tagcatcgga aatcggtgtg gcttatgcga aaggcatccc agtgatcggc ttatatactg
    47281 atactcgaca gcaaggcgga acccacccga aaaaaatcgc tgcactacaa gaaacagctg
    47341 aaaatcaatt tcactacctg aacttatata caataggttt gatcaaattg aacggaaaag
    47401 tggtttcttc agaaattgag ttgctttcag aagtaaaaag atttttagat ggagggactt
    47461 tcagtgatta aagaaatcaa aaaggtgcaa atagctttac ttgcttttgg tgtattcgtt
    47521 gtctgctata atttttatga atttatcaca caaaaatatt cgacttctca aggaatcaca
    47581 tttatcgtgg aatctttatt agggattgca ttgattttca tgccacaagt cattttgaaa
    47641 gttttcaaac ttaaaatacc agcagcaatc gttttgtttt actggttttt cttgttcatc
    47701 tctgtctttt taggcacagg gatgcatttg atcagtatca tctctttttg ggacaaaatc
    47761 ttacatgcgg tgagtccaat ggtcctgaca gctcttggct atggattgat tggttacttg
    47821 atgaaagatg ctgagatatc aaaaaccagt ccttggttgt tcctcttatt cggctttgcg
    47881 tttgctggac tttgcggcgt gttttgggag ttctgggaat tcttatgcga ccagttctta
    47941 ggcatgaatc ttcaacgttt tgctgcttct gatggcacac tgtttgtcgg acgagcagca
    48001 ttgatggata cgatgggcga cttgctgaca aatacgatag gtgctgcctt aatgggattg
    48061 tttgcatggt ctcaaagtaa aaaagatgag cgttactttg aaagttataa attagaaaaa
    48121 gtgaaaaata cctaataaaa gaggttgtga cagaagcggg cagcttcaag aaatttcggc
    48181 ttatttttcg aagaaactac ttctggaaca ctgtttattt gaaaaaagga ggtctgacac
    48241 tagttgtgtc agaccccttt tttcttttat agtctcaatg gaagattaga cgatttcttc
    48301 tagctcttct gttttgaaat gaagttgtcc atcaagtaaa gtaatcgtta cttttgattt
    48361 aggcataaca tgtcctgcaa cgatttcttt agctagtggt gtttctactt ccttagtgat
    48421 aaatcgtttc aatggtcttg caccatatgc tggttcataa gcattttcag caatccacgt
    48481 tttggcttca tcactgattg tcagcaggat ttcttgatgt tccagacgtt gtgccaattg
    48541 cgccaccatt ttgtcaacga ttcctttcac gttgtctagg ctcaatggtg taaataaaat
    48601 cgtgtcgtcg attcggttca agaattctgg tttgaagttt ccacgtaata gcgtgttgac
    48661 ttgttcagcc actgcttctg gaatcgttcc atcagcagtc acgccttcca gcagtaactg
    48721 tgaaccgata ttgctggtca tgatcagaac cgtatttttg aaatctacta ctcgcccttt
    48781 tgaatcagtc aagcgtccgt catctaggac ttgtaacaag atattgaaga catcgggatg
    48841 cgctttttcg atttcatcca acaagacgat tgtataagga tttcgtctga cagcttccgt
    48901 taattgacca ccttcttcgt aacctacata gcctggagga gctccgacta gacgagacac
    48961 agcatgtttc tccatatatt cactcatatc gatccgtacc atatgatctt ctgagtcaaa
    49021 caagttctca gctaacgctt tcgctaattc ggtttttccg acaccagttg gtcctaggaa
    49081 aaggaaagaa cctagaggac gatttgggtc ttgcaatcca gcccgtgaac gaattaccgc
    49141 gtcactgacc gcatcaactg cttcatcttg accaatcact cgtttatgaa gtgtttcatt
    49201 caatttaatt agtttttctc gttcaccttc tacaagtttc gttacgggga tacccgttaa
    49261 tctgccaacg acttgagcaa tctcattttc ggtaacggac tcttgaacca tcttaatctc
    49321 gctgtctttt gctttagctt ctaattcctt caattctttt tctaattgag gtatcgtacc
    49381 atgacgcaat acagcagcgc gttctaaatc atagttattt tctgcatctt ctaattcgtg
    49441 ttttgcttta tcgatttcag cacgttttgc tgaaacagaa ttgacttctt ctttttctgt
    49501 ttcccactgc atcttcatcg catttgcttc ttcacgaagt tcagctaatt cttcttgcaa
    49561 atttttcaag cgttttttac ttgcatcatc tgattctttt ttcagtgcag cttcttcgat
    49621 ttccaactgc atcaaacgtc tagtaacttg atctaattcc gttggcatag agttcatttc
    49681 tacccgaatc gttgcactgg cttcatcgat caaatcgatt gctttgtccg gtaagaaccg
    49741 atcagtaata taacggtcag ataaagtagc tgctgctact aatgcgttgt catggatgtt
    49801 aacaccatgg tggatctcaa aacgttcttt taatccacgc agaatactga tcgtatcttc
    49861 cactgttggt tctttgacta ataccttttg aaagcgacgt tccaacgctt tgtctttttc
    49921 catatattga cggtactcat ctaaagttgt ggcaccgatc aaatgtaatt caccgcgagc
    49981 aagcattggt ttcaacagat ttccggcatc catacttcct tctgtttttc ctgcccctac
    50041 gatattgtgg atttcatcga tgaacaagag gatacgtcca tcactttttt tgacttcttt
    50101 caagactgct tttagtcttt cttcaaattc accacggaat tttgctccag cgatcaatgc
    50161 acccatatct aatgaaaaaa tcgttttatc ttttaaattt tcaggcacat ctttgcgtac
    50221 gatacgttga gcaagccctt caacgattgc cgttttccct acaccgggtt ctcctatcag
    50281 aacaggatta ttttttgttt ttcgtgaaag aatacggatg acatcacgga tttcttcatc
    50341 acgaccgatg attggatcca tttttccgct tttgacttgc tgtactaaat caacgccgta
    50401 tttttctaat gctttgtatt gttcttcttg gttttgtgag gtcactcttt ctcctcctct
    50461 catctcttcg atgtttttac gcaattcttt ttcagataat ccgtttttat tcaaataaac
    50521 agttaacgga taatttttta atttcatcaa cgctaaaata acgacttccg ttgaaagaaa
    50581 ctcgtcgccg aagctttcac gtatttggtc tgcttcattc aataatcgaa agaaattctg
    50641 gctgaggttt tgtccatatt ggacgtttcc accagaaaca actggatact catccagtag
    50701 acgatcaatt tcatgttcaa aagattcgac atccaatccg gcatccgtat agaaatttcg
    50761 accaaaatga ttaggttgta aaaagatttt ccatacatgg gcaatgtcga tatcttgatg
    50821 ttttcgagtg accgcaatct gttgagcttc agcaatcgct tcttgaagcg tcgtagtcat
    50881 tttctcaata ttcatacagg tataccctcc taattaaagg taagatcact tatttctttg
    50941 atctttacaa aaactattat atcgctttgg tcaattttgg tcaaaatata cacatggttt
    51001 tttgactttt tttctcgact tatataaatc ttaaaaaaac aaaattgatt gtatgtatat
    51061 aaaaaaagag ccaaacaaac gtaaaattat cttatccata acgtttttct ttgatttgat
    51121 aaaaagggta gatcttataa aagacctacc cttgattttc tttattctct ttttttactt
    51181 ctattatttc ttctttgatc actcttcgcc gttcagcaat cacatacaca aatccataga
    51241 gaaatggcaa gataaaggaa ctgaaacgat acagtaaaag cgaagaaact gcgtcaactg
    51301 ttccaacaac tggtctgaat aataacaaat agacaaattc aaaggaacca ataccagcag
    51361 gcgtaggaat aacccctgaa agcactactg caaagctgat aaacgaaatg gtcagtagaa
    51421 aatctatttt aggatggttt tcaatcagaa tcaaataagg aagtacatac caaaatacaa
    51481 gttttaacat attccataga taaatccgca atagcgcagt cctgtcttgg ataatggttt
    51541 ggaccgtttc ccttaaagag tagatctgct gattgcatgt atccacccat ttccgcaaac
    51601 gctcactttt aaataactta ttcgataagc tgacaaaaaa tatttgaaca ttcaagctga
    51661 cggacatcgc caataaaaag gcaacaatca cgatcgtcaa aaggatacca gcgatgataa
    51721 accagatcat tgatggacca ttagaataaa aaagagagaa ttggatgacc aagccagcta
    51781 atgcataagt gatcactgcc agtttgtaca taatcatatg gagtgctgtc acccctgctc
    51841 cctgggatag cttcattcct tttttgttat aaaaataaat ctccgagatc agcgttcctg
    51901 taccaaacga aacgattcgg taaaacgcaa tatagctaga ggtcaagaac ccatctttcg
    51961 ttgtaaaatt cggcgcgaaa ggttcagcaa tttctttgat tgagcgccct tcagcgattt
    52021 ggtaaactgt ccctaatata acagccgctg ccaaaacaac aacactggta gacagcaatt
    52081 cggcaaagat atctgcaaat gattgattga ttaaataata aataatggca aaaatcacaa
    52141 tcagcaaaag cagattcaga atgattttag ttttcgttcc tttagacgac atctcctgct
    52201 ccttaatgga aaaaataaat gagaatcaaa tcaacaagta cacctgcagc gaatcctata
    52261 gtaaacttct ttttccgagt tttatgatgg aaaacttttc gagagatcaa tcctcccaaa
    52321 ccaccgccta acaggctgac aaatatcaaa ttattctcag gaacacgcca gccgctagtt
    52381 ctagcctgat gtttatcata ccccatcaga caaaataagt aaaaattcac aatgaaaagg
    52441 taaaaccaca aaagattgtg taacaataca tccattactc ttcttccaac tttctgttaa
    52501 ctactatttc taagcttttt cttctttctt tggtgaataa actgttaatc cttttgatac
    52561 aacagataat tcaactggaa gatcatctgt cgtgtctcca tccgttcgag tgcccacaga
    52621 tttttcctgc gcttggattt tcatgcgttt ggctgtgaaa taagtaatgc cggggatcgt
    52681 atagaatttg cctctcatga ttctaggcaa ataaaaaata aatcggaaaa tatctaagga
    52741 aggtacgatc gttacatgaa attttccatc atctagggtc gctgagtcgt caaaatttgt
    52801 aaaaccgccc acggagttag acatcgtgac tgtcaacagt tgggattttc ctttccaatg
    52861 ctcctcgtct gtttctaaat ccaaatgata ttttttcttc ttcagcaata acttaaaaaa
    52921 cttcttgata aagatccacg agccgtaacg ctgtttttct tcttgactga tccaaactgc
    52981 tgtatctgct aagattccga ttgtcagtgt agagataatg acttcatcat tgacttgacc
    53041 atagtctatt ttccgtacat tgccttccag gatcacgtca gcagcttctt cttttttgag
    53101 gggaatctca agaacacgtg caaggttgtt gacagttcca cctggaagta atccgacatg
    53161 gtattgagat aatgtttttt ggaacgcttg gacgatatga tgaatcgtgc cgtctccacc
    53221 tataattact aaagtatcag cttctatggc ttctgctttc ttgactaatt ccgcatcatc
    53281 aatcgatggg ccagtttcag ctagttctac atataattct ggctgctttt cttttacata
    53341 atcttgaaac cagcgtgcta atttctcacc ctcatctttc cctgaacttt tgttataaaa
    53401 aactagcagc ttttccattc tttacctcct atttactctt caaaaaagag ggcgggacaa
    53461 ccaattccat tgtcctgccc tttagcctta gacagatgac attccatcat tgggacgaac
    53521 gccttgtcat aagctgttct ttttcgattt taactctttg tgttcaattc tgcaattttt
    53581 acaatcacgt ctgttgcttt ttccattact tgtaaagaaa caaattcgaa tcgtccgtgc
    53641 atattttctc ctccggcaaa tagatttggc gtcggaatac ccatgtaaga gatttttgaa
    53701 ccatctgttc ctccacgaac tggttcgatc actggagaga taccaagctc gatcattgct
    53761 tgtttcgcta attccacgat gctcatatct ttttcaatga tttctttcat gttgtaatat
    53821 tgatcataca tatctacttt gattcgttct tgatcaaatc gctgattcaa ctcttcttgg
    53881 atatgtgtga tttgtgcttt gcgtgcttcg aatttttcac gatcatgatc gcgaatgata
    53941 taactcatag aagcttcttc aggattccca tccaatgcca tcaaatggaa gaaaccttcg
    54001 tatccttccg ttttttctgg aacttcatcg gctggaagct gattatgaaa atcgattgct
    54061 aactgtagag cattgatcat tgtatctttt gccgtccctg gatgtacatt tttcccttgg
    54121 atcgtaatat ttgcttgagc agcactaaac gtttcatact gcaactcacc taaaggacca
    54181 ccatcgactg tataagcaaa atctacatcg aattgctcaa catcgaattt atccgctccg
    54241 acaccgattt cttcatcagg accaaaagcc actttgatgg taccgtgagg gatagaagga
    54301 ttattgatca agatctccat tgcagtcatg atttccgcaa tccctgattt gtcatctgct
    54361 cccagtaatg tactgccatc tgttgtgatc aatgtgtggc cttcataatt tttcaaattt
    54421 ggaaaatctt ttgtattcaa tgtatagcgt tcttccgcat ctagtacgat cgtagactcg
    54481 ccatcatagt tttcaatgat ttgtggattg actcctactg cattaaaatc tgctgtatcc
    54541 atgtgggaaa taaatccaat ggaacgcact tcatgatcaa cattgcttgg taatgttccc
    54601 ataacgaatc cgttatcttc attatagaaa acatcagcta agcctaactc ttctaattct
    54661 tttttcaatt cttgcgcaaa agcaacttgt gtctgagtcg atggagtcgt tttgcttgtt
    54721 gcatcggagc gtgtttctgt tttgacatat cgtaaaaaac gtggcaaaag attttcgtac
    54781 atagtattct ctccttttaa ttaaattgaa atgggtttgt atttgtttca gaaacgaaaa
    54841 agttaatatc ccaattttct tcttgtttcc aagattcaaa tttctcgata aatcgttgct
    54901 tgcataacga ttcaatataa tgaccgggat caattgcaat caacccagcg ctttgcatat
    54961 catgagccgt atgataatag atatctcccg tgatatagac atctgctctt tgggcgattg
    55021 cttgcggata aaatttttca ccgcttccac cgcaaatcgc aatgcgcttc acagaggatt
    55081 ttgcattttt tggctggact attctcagtc catctagttg gaacgcttct tttacttgtt
    55141 ctacaaatgc ctctatagaa atttcttgcg gcaattcacc tactcgtcct aacccaaaca
    55201 tttcgactgg ttcatctata gcgaaaagat cataagcagg ttcctcataa ggatgagctg
    55261 accgcatggc ttgaatcact tgtttttcga tagtttccgg caaaataacc tcaacttttg
    55321 cctcttgaac ttgttctgtc ttccctactt tcccaatcgc tggttgtgca ccagcttccg
    55381 gagtaaatcg accgtgtcca atcgaggtaa aacttgtacc ggtataatca ccttgtgtac
    55441 cagcaccagc tgcagctaat acttcacgca ttttctgtgc atgatccaca ggtacataaa
    55501 cggccagttt tttataatga atctcatgag tctttactaa ataactctcc acttcaatac
    55561 cgagaagttc acaaaaccag tcattcagtc catcccaaat aatatccatg ttcgtgtgtg
    55621 ctgcatagac ggcaatatca tgtttcaaca aatctgcata cattttttct tgtggctgat
    55681 cagtaaccag ccgttttaca ggacgaaaga ttggaggatg tttagcaatc agcaaatcga
    55741 tttttttctt gattgcttct tcgaccactt ctggacgaac gtctaatgtc atcattactc
    55801 gttggattgg cttatccaat gttccgatat gcagtccgac tgggtctcct tcttcagcta
    55861 gccactgagg acaatagctt tcaaactttt ggatgaaagt gcgtccgctg attgtcatgg
    55921 ctttaaaacc tcctcaatca tttggatttc ctgctcgatt tgtttttgac gtgcctcttg
    55981 attagctgct tgtttgagct gttcacggat agcttttctt tgttgaagct ctcttgtcca
    56041 ttttgatttg aagatctcac ttttctcttc aagcagaaat ggcccgaaca tcagctccat
    56101 ttcattgtaa gtaactggtt tttgttcttt ttcggctaca atgatttcat atattttttt
    56161 attttcttca agtatttttt ctgattggat tttatactga ttttcttgca gccaagatcg
    56221 taatgtacgc tctccgatgt ttggctgaag gatcaaacgt tcttgtccgc ttaagcgttg
    56281 attcaacttg ccagcttcta ggatatctcg gatcagtgaa cctcccatac ccgcaatcgt
    56341 gacagctgta ataccatctt gtggctggat agcatctaga ccatttgcaa ggcgtacggt
    56401 gatacggtct tctaatccat cttttttgac ttgcttacgt gctgattcat aaggaccttg
    56461 aacgacttca ccagcaactg catattcgat ttttcctctt aatatcaatg caacgggtaa
    56521 gtaagcgtga tctgatccaa tgtctgctaa acgagcggat tcagggacaa agtcacccac
    56581 tgcggctaag cgctgagata aatctaaatg gttcatagct tcactccttc tagataatta
    56641 tagcattttc caaataaaat aaggagacta ggaa
//
LOCUS       DAFELO010000009        56413 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFELO010000009
VERSION     DAFELO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.009"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..305
                     /locus_tag="LP452_000643"
     CDS             <1..305
                     /locus_tag="LP452_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2013397.1"
                     /translation="ITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(1171..1701)
                     /locus_tag="LP452_000644"
     CDS             complement(1171..1701)
                     /locus_tag="LP452_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL2013398.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1698..2315)
                     /locus_tag="LP452_000645"
     CDS             complement(1698..2315)
                     /locus_tag="LP452_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2013399.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2897..3118)
                     /locus_tag="LP452_000646"
     CDS             complement(2897..3118)
                     /locus_tag="LP452_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL2013400.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3296..4828)
                     /locus_tag="LP452_000647"
     CDS             complement(3296..4828)
                     /locus_tag="LP452_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2013401.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4964..6049)
                     /locus_tag="LP452_000648"
     CDS             complement(4964..6049)
                     /locus_tag="LP452_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2013402.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(6064..6783)
                     /locus_tag="LP452_000649"
     CDS             complement(6064..6783)
                     /locus_tag="LP452_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2013403.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6780..8090)
                     /locus_tag="LP452_000650"
     CDS             complement(6780..8090)
                     /locus_tag="LP452_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL2013404.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8117..8707)
                     /locus_tag="LP452_000651"
     CDS             complement(8117..8707)
                     /locus_tag="LP452_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL2013405.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(9025..10086)
                     /locus_tag="LP452_000652"
     CDS             complement(9025..10086)
                     /locus_tag="LP452_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2013406.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10654..11721)
                     /locus_tag="LP452_000653"
     CDS             complement(10654..11721)
                     /locus_tag="LP452_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2013407.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11741..12220)
                     /locus_tag="LP452_000654"
     CDS             complement(11741..12220)
                     /locus_tag="LP452_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL2013408.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12237..12698)
                     /locus_tag="LP452_000655"
     CDS             complement(12237..12698)
                     /locus_tag="LP452_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2013409.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12712..13962)
                     /locus_tag="LP452_000656"
     CDS             complement(12712..13962)
                     /locus_tag="LP452_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL2013410.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14340..15791)
                     /locus_tag="LP452_000657"
     CDS             complement(14340..15791)
                     /locus_tag="LP452_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL2013411.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(16112..16876)
                     /locus_tag="LP452_000658"
     CDS             complement(16112..16876)
                     /locus_tag="LP452_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL2013412.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16904..17602)
                     /locus_tag="LP452_000659"
     CDS             complement(16904..17602)
                     /locus_tag="LP452_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL2013413.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17614..18393)
                     /locus_tag="LP452_000660"
     CDS             complement(17614..18393)
                     /locus_tag="LP452_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL2013414.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18409..19353)
                     /locus_tag="LP452_000661"
     CDS             complement(18409..19353)
                     /locus_tag="LP452_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2013415.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19399..20178)
                     /locus_tag="LP452_000662"
     CDS             complement(19399..20178)
                     /locus_tag="LP452_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013416.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20558..22633)
                     /locus_tag="LP452_000663"
     CDS             complement(20558..22633)
                     /locus_tag="LP452_000663"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL2013417.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22635..23552)
                     /gene="glyQ"
                     /locus_tag="LP452_000664"
     CDS             complement(22635..23552)
                     /gene="glyQ"
                     /locus_tag="LP452_000664"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL2013418.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23938..24756)
                     /gene="recO"
                     /locus_tag="LP452_000665"
     CDS             complement(23938..24756)
                     /gene="recO"
                     /locus_tag="LP452_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL2013419.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24899..25798)
                     /gene="era"
                     /locus_tag="LP452_000666"
     CDS             complement(24899..25798)
                     /gene="era"
                     /locus_tag="LP452_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL2013420.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25813..26208)
                     /locus_tag="LP452_000667"
     CDS             complement(25813..26208)
                     /locus_tag="LP452_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL2013421.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26192..26668)
                     /gene="ybeY"
                     /locus_tag="LP452_000668"
     CDS             complement(26192..26668)
                     /gene="ybeY"
                     /locus_tag="LP452_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL2013422.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26697..28889)
                     /locus_tag="LP452_000669"
     CDS             complement(26697..28889)
                     /locus_tag="LP452_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL2013423.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28913..29884)
                     /locus_tag="LP452_000670"
     CDS             complement(28913..29884)
                     /locus_tag="LP452_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL2013424.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30589..31968
                     /gene="brnQ"
                     /locus_tag="LP452_000671"
     CDS             30589..31968
                     /gene="brnQ"
                     /locus_tag="LP452_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL2013425.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32124..32570)
                     /locus_tag="LP452_000672"
     CDS             complement(32124..32570)
                     /locus_tag="LP452_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL2013426.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32597..32773)
                     /locus_tag="LP452_000673"
     CDS             complement(32597..32773)
                     /locus_tag="LP452_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL2013427.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32935..33363)
                     /locus_tag="LP452_000674"
     CDS             complement(32935..33363)
                     /locus_tag="LP452_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2013428.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33455..34315
                     /locus_tag="LP452_000675"
     CDS             33455..34315
                     /locus_tag="LP452_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL2013429.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34409..34759)
                     /locus_tag="LP452_000676"
     CDS             complement(34409..34759)
                     /locus_tag="LP452_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL2013430.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(34775..36502)
                     /locus_tag="LP452_000677"
     CDS             complement(34775..36502)
                     /locus_tag="LP452_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL2013431.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36715..37641)
                     /gene="trxB"
                     /locus_tag="LP452_000678"
     CDS             complement(36715..37641)
                     /gene="trxB"
                     /locus_tag="LP452_000678"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL2013432.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37786..39222)
                     /locus_tag="LP452_000679"
     CDS             complement(37786..39222)
                     /locus_tag="LP452_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2013433.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39219..40622)
                     /locus_tag="LP452_000680"
     CDS             complement(39219..40622)
                     /locus_tag="LP452_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2013434.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40852..41754
                     /locus_tag="LP452_000681"
     CDS             40852..41754
                     /locus_tag="LP452_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2013435.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41795..43681)
                     /locus_tag="LP452_000682"
     CDS             complement(41795..43681)
                     /locus_tag="LP452_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2013436.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43778..44630)
                     /locus_tag="LP452_000683"
                     /pseudo
     CDS             complement(43778..44630)
                     /locus_tag="LP452_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44965..46749)
                     /locus_tag="LP452_000684"
     CDS             complement(44965..46749)
                     /locus_tag="LP452_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013437.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46749..48500)
                     /locus_tag="LP452_000685"
     CDS             complement(46749..48500)
                     /locus_tag="LP452_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2013438.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48642..48866)
                     /locus_tag="LP452_000686"
     CDS             complement(48642..48866)
                     /locus_tag="LP452_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL2013439.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49118..50704
                     /locus_tag="LP452_000687"
     CDS             49118..50704
                     /locus_tag="LP452_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL2013440.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50770..52545)
                     /locus_tag="LP452_000688"
     CDS             complement(50770..52545)
                     /locus_tag="LP452_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL2013441.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52756..53658)
                     /locus_tag="LP452_000689"
     CDS             complement(52756..53658)
                     /locus_tag="LP452_000689"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL2013442.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53937..56282
                     /locus_tag="LP452_000690"
     CDS             53937..56282
                     /locus_tag="LP452_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL2013443.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16724 a        11739 c         9136 g        18814 t
ORIGIN      
        1 ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg
       61 atattgatat atattttgcg gaccctggaa caccttctca acgcccatta aacgaaaatt
      121 ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat agattttaga gaagtgaatc
      181 agacatttat ttccagtgtc agcaatcaac gcaatcatat tccaagaaaa tcattgaatt
      241 acagaacacc aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa
      301 tttgacaaat catataattt aaaattaaca ttgattctcc tatgaaaata tcttttacct
      361 ttctcctgtg aaaaggaaat ttcctttttt atagtgttat tgatataatc agaatattca
      421 tcaaactctg tatctggagt taaaaatcta cagtaacatc tataagcttt gctagtaatc
      481 ttcttgggtt ctctctccat tgtcaagcaa catcaaaaca cctacttact attcgattct
      541 tttctgccaa aaacgagtca gcatcactca caacttatgt gttgctgact ccgactagtc
      601 ctttgttcaa tatcaactta ttaaacagat ataatatttt tctttatttc gtatactcca
      661 agacttccat ctttctttca tcttatctct gaaaaaaaca cagcaaagtg cccttaataa
      721 atatacatta gaatttgcaa attttttcat cttaagatat atcctattca tattctttac
      781 aatcctaatg ggtgattaca aatctattta gttcaagttc tacattataa acacctttct
      841 tgatagtatc atctgctgca caagcgatgt cgatccactt attaggggat ttacgtgctc
      901 aaaagtggct attccttttg ggaagtccat ccataataat ggtccatgga tttttttagg
      961 gtcttgtcgt tcaaatttct caaaaacttt attatacttc catagtttta taattatttg
     1021 gtttcacttt ggctcgttca aacaccaact tgtatttctt ttcaacgttc gaactggagc
     1081 cccccctata atctcattat tattttcatg actttttact accgtggaat tacaagcaac
     1141 aacaatatta tctgctaaat gaacaccttt ctatatcacg ctttttgctc caatccaaca
     1201 atgtttacct ataactattt tattattttt attcaaaatc ctatgagtat tcaattcaaa
     1261 aatcgtatgt gaatcggtat ccataaataa tgtatcccac gatactgtac agccatcttt
     1321 gaattgaatt tccttaaagc aaattatact catttttcct gtcgaattaa actttgaccc
     1381 aatatctaaa actgcatttt tccttatgtc aatatgagcc ccttgtgcga taaacgctgg
     1441 cccttgtatt cttagtattc cttcaatact tataatcgtt tcttcatttc ccgcagtatt
     1501 taatactgga aagcccaaat aaactatttt tccagattgt aatactatct caattttccc
     1561 tctcgatttg cgtgtaatcc gtactttacg atgcaccaaa ataggtaatc tccacgcatc
     1621 tgaaatatga agaaatttat aattgaagta tatcgtttta ggtaaattta atatattctt
     1681 tattatcaat ccgagtctca tctttcacca tctctttttg taatcaaaat tagtttataa
     1741 aggattacac ccaatatacc gcccaacgta ttatatacaa aatccgaaat ttgaaacgtt
     1801 cctagcctca aaaaaagttg taaaaattca attattgctg aaaaataaaa tgcaattttt
     1861 cctccatata ctattgagga ccatattttg ggtttcttta tattttcata tttaatagac
     1921 attaccatat aaacatatgg aataaaaaga ataatatttt ctaaacacgc tgtagataac
     1981 tcttttgttc cagttgttgt attatactta taaatccacc agtctcccat aacatcagat
     2041 aaaggattta accaaattgt tcgattgatt aatgttgtaa ataaaatcat cgcagtatag
     2101 aatatcaaag cacaaacttt tctatatttt ctatccttct ttaaatgaag ataaagttcc
     2161 attagtatac gtttatagcc ctcacctgca gcaatgccat ctgttgcata cataaaatag
     2221 accagtccta aaattgtcaa taaaacagca aacccaaagg gttcatataa tttatataat
     2281 atatctgtaa taatttctgc aatcactatt atcataatta tttctccaat atcaagtata
     2341 cacttatcgc ttccaattac acatgatcat attagagtgt attatcacat tctctaatat
     2401 tcaatttatt tttcctaaaa tattcgaaat aatcgtcttg ataacgagta agtattttta
     2461 tacgattgat aacatagtaa caatattttt aattatagta aaaaatcatc tgcctttttc
     2521 atccacctaa tcttattttt cattttattc aacatcctta aataaacttc cgttcctaag
     2581 ctctataata tcttcttaaa aaataatatt atggtgtcgt tttcaaactc gtaatctttt
     2641 acattggaaa aatacatact tttctcaatg tagcaactcc tatctcacag tattcaaaaa
     2701 ttcttcaata ttatgaaata cttgtgcatg ttctttaaat accttgtcag tctgtttata
     2761 atcccctact aaattttgca ttgctcccaa ctcttcgaca aattgtcatc tccatctgta
     2821 aacagcccta aaaactcata taatgctttt tcatcaatat ttttcccata ttattctcgg
     2881 tatagtttca caagttttac ggattcttca tattgtttat ctttttataa tattcatcta
     2941 gggacataat tacttttgct agatttcctg caacaacaca atcgtctgat acgtccttgt
     3001 ttactaaact atttgcacct ataactacat tatttcctat atgtacaccc tttagtattc
     3061 tagacttcat tcctataaaa acattattac ctattttgat cctctccgat gatcccaaag
     3121 cttctccata ctacttttaa aaccgatcaa tcataaacat gagtcaaaat tgtaactcct
     3181 tcatatcatc tccaatttca acaagagtct tcgttggaac aaaaactata catcgttcgc
     3241 ctattcggca gcctaaaaat tgtagttact ttatatatca ttctgatgtc tcttctcatc
     3301 tttttaccaa taccgacgta agaactttca aaatatactt aaacttctta ttacggatat
     3361 taaacagaag aataactaaa atcgatatga caaccgatat aactaaattt atcactaaac
     3421 ttatcacttt gaaatttccg atattaacat acgaacaagc aaaataactt aaataagaag
     3481 caacaaaagc tattacacac aaacttatga gcttccaaac atatttacct gccgattcaa
     3541 atttaaatat attttggaaa acattatata ataaccaggg taaatcaacc aacaatactg
     3601 aaacaatggt tgccgccaaa actccatata atccgaacca attaacgagg atgatattta
     3661 atacaatgtt tatcattgct gtaattaatg gtctaaacct gtctgcgtac catattccag
     3721 cagcatcttt gtataaccca attaactgat caatttcata taagaaaaaa tgaactatca
     3781 taaatataac aaatgaaata ctcataagat tatcacttcc cacccagata atcatgaacg
     3841 gctgaaataa tgtaataaag catgcacaac taattgccag aaaccaacct ataattgttg
     3901 taatatcaca aaaatctata taattttttt cttgactttc tacaataaga ctgttaccta
     3961 tacctgctat acatgattca taaattattg caaatatact cattatggca gaaataataa
     4021 agtaataatt ctgataaatc gcaagtactg aaagacctaa gaaagatgaa ataataattg
     4081 aatctgaaga tcgtaatatt gtaccgccta ccttgtttgt aactaatcct tttacttttt
     4141 ttataatatc gtcaatagtt tctttctcta actcaccaac aggagtataa tcaggataca
     4201 tcttttgaga aacaacagca cacattaagt taagaactat ttgtcccaaa atagaagcca
     4261 ataaatacaa atagtaattt tttaagaaaa tcagaacata tgcctgcatt acaaattgga
     4321 atgtaaaagt aattagcata gacacgttaa ttacatcatt tctttgatgt gcatatatta
     4381 gactctttct atatgaaaat aaccaatacg acaaaacagt atttatcaaa tacattaaat
     4441 acaaaactgt catatttaaa tttccaggaa tcgaaccttt aactaaaaat ctcaaaaaag
     4501 gaatgcataa caatcctaat gtaaacacta tccatccaat aattgaaaag tactttttat
     4561 atgcgttcag taaagcacaa atctcttttc tattgtcttc tgctataggt ttatacatac
     4621 ttacagttag tgcagcacct attcccaatt ccgctaaatt taacatttga aatatagatg
     4681 tgaacaggtt gttcaatcca gcatactcca ttcccatctg ataaatcata agtgtacgca
     4741 taaaaaaggg aatgataatc tgatataact taaaaattgt tccagtaatt atattccttt
     4801 tagcattttg tttacgatta attatcatat catatggctc caaactttct attttttatt
     4861 gttacctaat actatataat ttctaaaatc ttgaaatgta ataggtttct tctagctctt
     4921 tgattaatta cgctaatttt catgtttgga caaccacaaa aaactataca ttttcaatca
     4981 aagcaaccca ttgttttgta atttctttca aattaaattt ttgtacatta ttatatgcgt
     5041 tcagtgacat tctcattgct aaatcagtat tcttagtaaa cattatcatg ctcttagcta
     5101 attggtctat attcttatct tcaaccaaca tcccattaaa attatgatgt accaaatctc
     5161 tcggaccagt tttacaatca taagaaataa ctggtaatcc ataagactgt gcttctaata
     5221 taaccatagg aaatccttca taaacagaac taagcacata acaagatgca gatttataat
     5281 attcctccac atggtctgaa tgccctgcta tagttactct ttgcagattt aaatctttta
     5341 tctgctggcg aagatctttt tctaattcac cttttcctac aatgataaga tcccaatcat
     5401 ctactttttt ttctacaatt ttccaagcac ttatcatgtc agcaaaattt ttctcttcca
     5461 ctaatcgccc tactgcaaga aatctctttt tatcataatg atattcgtac tcgatattag
     5521 atatacacgg atttggcata gttataacaa aatcttctga taaactatat tcatcaatat
     5581 gtttttgacg atcttcttct gtgagaacca caagcctctt tgcatatttt tttatcaatt
     5641 ttttagcctt gaatctcttc ttttctttca gcatttccat tgtccagtaa ttaaaatgtt
     5701 cccaagaaat caatttgcac tttgttcctt taattgccct tgtagtatac aaagacaaca
     5761 aagtatcaat atcaattact atatcaaatt catttttgca tatatacttg tgcaatttcc
     5821 aaaccggata taaataagtt ctatataatt ttccctccgt tttaggattt aacatataat
     5881 caaccttaat cgtcttatct aacccaaaat aaggctctcc actattccaa aagcttaaaa
     5941 tagtgacatc ataacctaaa tcagacaagc tattggcaat aatactccct actctttcag
     6001 ttcccccaga acgagaaatt acaccactaa tgaaacaaat tttcttcaaa gtacttttct
     6061 cctttataag tgtaaaaatt ttttcagtgc aagtaacctg ttaaattttt tttgtccaat
     6121 tgttgatttt aatctataaa caaatttttg tttaggtgtt ttccaagatg cattatattg
     6181 atgaatgctt attgtattac tagttttata aatttttcca gtatcataat ccttggcagc
     6241 aaaataatcc gtaggataga tatgcaggcc atttattatt tgttcgctgt tatcgcgaac
     6301 caatccaata gattcaagat aatctgtaat ataatacgga ctcggtttca agttcaatgt
     6361 accatcattt tgataaaaac ttaatgaatc gtaaatatcc cttaaacctt taataacaga
     6421 atgtccttct tctgcaccaa aaccaatacc gccattaacc cagatttccc cttcaaatcc
     6481 catataggca tgattttcac gaagtatatc taacgactta attagctcaa catctgtatc
     6541 taaataaaat ccaccatgat tataaataat atcaaatctt gcataatctg gaacaaaacc
     6601 ccatttacgt tctttatatg catcggccat ataacggttc ttatttaaat cataatttga
     6661 ttcattccat tcaataatct cataatcagg gcaatacttt ttccagcttt ctatgcactt
     6721 tctatctttt ttcggaagtg gatttccccc gaaccaacag taatgtatct tcttaggaat
     6781 cataatatct ctccttttgt atttttttca ctataataat gctctttcct tttcacaact
     6841 tcaggcatgg ctaaaatcat ataaatcagt ggccccgttg tttcacccct aagtgttgtg
     6901 ttatacacaa aaaagaatac cgacataatt gcaataactt taacaagact tgcatcttta
     6961 tccttcataa aagatctttt tgcattgaaa aatgtcttta ttataaaaaa taggaatatc
     7021 gaaactccta atattccaac ttctataaat aaccaaatat gatgaaaata ctgataatga
     7081 agattaccaa acgatttata aaactcactg acaaaaaaat tggtaaactc tgcattgcca
     7141 atgcctactc ccattaggac taatctaaaa ttattattaa atatttcatc tactactcta
     7201 gagataccag ttattcgatt taaatcacca acaccatcat atccagaatc tcttgacgca
     7261 tattctaaaa gaccttgaat tgtgaaatta tcagcatagc tttgtccatt atcgttcaat
     7321 gtaataaata ttgttataaa aaaggataat actatcgaca caagtattac gcatgataat
     7381 atcttgatca ctactttaaa atcaactttt aaagtgaata ctattgcaac aaatattata
     7441 aataattcag gaataaaaac tttcaattca gctaatgcag acataaaaag tgctgcacaa
     7501 acacataaaa taaaatggat gaaatttttt tctcccttga aatatccgat tgtaacaaca
     7561 gtaactgtca cacacaaaat taaattcaat ggcatattac aacctgaata attccaaaaa
     7621 actccaccta tcatatctcc tacaattgtt ccagttggat aggtgaccat gaatctttca
     7681 attaaacaaa gtggcagtga aatccagaat aatttttcaa ttatagcgaa tactttatag
     7741 taatctttaa ttgttaaata tgttgcacaa tttatatata ctatgaacat acgagtgtta
     7801 tttcgtatcc cccagataat caatcctata ttttctaaat taataactga gctacaaata
     7861 gaaactagta aaaacaaaac tatcagcatc tcatttttgc tagcatatat tccttttctg
     7921 cgtctaaagc taagtgcatt taataacaca aagatattaa ttatatctcc caaatataaa
     7981 attgctcttg gggcgttaaa agtatataca agaactatgg agattgcaaa aaatgccaac
     8041 gtgaacaatg tccaatctgc tcctgaccat ctttttattg aaatacgcat cgttattcac
     8101 cttctttcgc taaaaattat tctaaaaata cttttgttaa attaacatac cctattattt
     8161 ttgccggtac acctgcaaga attgcaccat tctctagaca atctttgata acaatagctc
     8221 ctgccgcaac aactacattg ttagctattt ttacattacc aataatcttt gcacccacgc
     8281 atacacgtac attatttcca attacaggac acttaggatc gattccatca tttccaatac
     8341 agttattccc atataagtaa caattttttc ctattatggc gttaccatta ataacaattc
     8401 cttgtgtatg gtaaaatctt acaccttcat caaatacatt ttcaccacac tctataccac
     8461 attttcttcc caaactattc attcttctgc aagacacaat atacataatc gcataaatga
     8521 tatttttctt tctctttgaa taataataag atgtaattct cattcgacga gtataccgcc
     8581 aagcgttgta gtccggatgt gatttcatta tttttagtaa ttttgtgtgt tgtcctttag
     8641 attctatata tagattcttt tccatacgaa gaaccgcttt taaatattct ttactgctaa
     8701 tttccattca tatctcctat taattcaatt tatacttaac aattttattc ggatttcttg
     8761 caacaagagc atcattcaat gctactgaat ctacaacgat ggatatgtta tcttcatcgt
     8821 tgattccacc ggatacaata ttacttataa aaatcgtaac attttttcca aaagaaggga
     8881 tgtccgtgat gtataatgcc cattttccga tatttccagt tatttaaacc taacgaattt
     8941 ttacaatagt acaagtatca caatcattaa tatataactg gaatataaat ctccaaaaat
     9001 tcactatagt acagtttttt atgattaaaa tctcctattt ctacccattc cgcttaaata
     9061 tgctaatgtt ttaaccccaa taatggattg gtttagtaaa aaatagttaa atattcgttg
     9121 tttaattgga ttttgtttat atgggtatga gtgaatacag gcaatcaact catcgttcat
     9181 aagtatctta gacaaatagg agtttaaatc aaattcttta ttaaaactag catatctaac
     9241 tgccttttga atacaagtac gacagcgtcc caacaaaagt ctccttgttt tgtttacaaa
     9301 tatctcattt tctaatatat agcttaaatc gccttcaatt tttttaacta attcaatatt
     9361 acaatcgaac tcattatctc taaaattatg tgtcaatgaa ttattattat tacatctata
     9421 ataatagata caatctttaa taaaacttgt tatttttgaa tgttgcaaat atgaaatatg
     9481 gaatatgata tcttcagata tataatccct ttctgacgga aacttgatat tgtgctgttg
     9541 aattatctca gatgaataca gcgcatgcca aactgacatt ggcaatataa aatcgacttt
     9601 ataactcggc tcagtgccaa tcattccaag caacactttt tcaattatag cgttatttgt
     9661 aaatatcgta ctatcatatc ttgctggctt tgaaataata ttattatcat cataatattc
     9721 ataatatcca gcataacatg tgtctgcatt actccttttt ataccattta acattgcttc
     9781 aatataatta gctgcaacaa aatcatcaga atcaataaaa gcaacatatt ttccttttac
     9841 atgttcaagt cctgtatttc ttgccaatcc caagccagca ttatttttat gaataatacg
     9901 aatccttgaa tcctttaatg catagtcttc acatatttta ttagatgaat ccttagatcc
     9961 atcatccaca attataatat ctaggttctt atatgtttga ttacacactg attccaaaca
    10021 acttcctaca tacttttcaa cattatatac aggtatgatt acactaatta gatcactcga
    10081 attcatcctt ctatcctttc actcacgttt tctcaattag tcaatgacgg catcaacgat
    10141 tttcttaaat tctctgaaaa aaatagactc tgatcttgat gatgtgaatg acatactatc
    10201 ttcttctaga aaaattattt cctttaaatt atttattaaa tcaataatat gatcctcgta
    10261 agtatgcttt cccttggaat aatgaaaata tacttttgta ttacacaata ttgcaatttt
    10321 atttcttaat atgattattt cactcattca ttactgccac atatacacaa cttataacca
    10381 ccccctgcaa agtttagggt aagttatcag ataatatctt cctcttcatc atccagaata
    10441 caaaatctat ttatttatag aaatatcatt aatgttttta tataatgtac cgtgcttgta
    10501 ctcttgctct attacaagca ctaaaacact actattcatt tgaattctta cacggataaa
    10561 aatttacatc gtctatttca atattttttc tctctatttt attcacttaa taagttcatt
    10621 acataatcga aacatttttt attttccttg tcatcactcc ttgctccctt tttgatacag
    10681 atttaaaatt ctatcaaaga aatttccatc gttaaatttc gttattgcca aatccttcga
    10741 acaacgtgaa aattttacgt atttatcttt atcattgatt tttagtattg cagatgtcaa
    10801 cgctgatacg tcaccaggtt gaaacaaata tccattttct ccatcacgta ctgctgaaga
    10861 aatatctcct acatctgtag caattacagg cattccataa gaaatcgcct ctagaataga
    10921 tataggcaat ccctcgttat aggacggtaa aatcatcaca tttgatcttc tatagcactc
    10981 ctcctttttt tctcccacaa cccaacctgt aaattcaaaa tacgattgca aattatattc
    11041 ttctatcaat tctttgagcg atatttcttc atcacctgct ccagcaatct caaaaataaa
    11101 ctttttttct ggatattgtt ctgcaatgtt tttaactgct tctaccaaat cgtgtacacc
    11161 ttttcgcttt atcaaaactc ccataaacag aaatcggcac atttttgaat tccactttat
    11221 ggtatattct ggaactacaa tagaattttc tacaacaaca gttttacaat ttggctcaat
    11281 ttctttaatt atcttcttcc atttttctcc aagtacaatc gtggaatcag actctcttaa
    11341 cagcaagcga atctgatttt gtttttttac agaaacacta tcataccatt ttttaaattc
    11401 cgatccatga agatggatga ttacaggaat atttaataat ctaaaccact tattcagcac
    11461 atatttccta ctaaaactcc ctctgtacga catgtgcata tgaataatat taggtttact
    11521 caataaagag atacctataa ttttagcaaa tgcatacata aaatataaag atttaaatat
    11581 tgcatttcca cccttataac tcggtataaa agtcatctcg atattctttt tttcccaatt
    11641 aaaattgagc atttgtgtaa ttatacttgt aattccacct tttaccgagc tgtcattacc
    11701 aatcataagt actctttgca taacgcttta ctccttatat ttatattttt tctattaata
    11761 cctctatcga attaataatc gtttccgtgt tagatacata cttgttaaac atccgaactc
    11821 ttgtttcttt aacggcatct tctaaatttt ctaattcata acaaggatag ataatattca
    11881 tctcatcaaa ttgtctaatt aactgcagtt ggtgatcatc gacatgctca ccatacttag
    11941 ccaatcgagg aacagcaatg acccttttac ctttttttac ggccccaatt atagcacctg
    12001 tacctccatg agtaatcacc atttctgctt tttgcatagt ttcagtaaac tcatccctat
    12061 ttagaaatgc tttataggaa taatttagtg gtttataatc actataacca ctctgtgcaa
    12121 atgtactttc gattattact cctttttcaa ttaaatcatc aatagctttt aacaatctat
    12181 caaattgaaa tttttgtgaa cctaatgtta taaatatcat ttttttctcc tattatttaa
    12241 tatatcccac ccaaataaat agcattagga taaaattgta gcatagtttc ccactgaaca
    12301 taaaattgat ctgcaaaatg atacatcaat tttcctgtct gagtagggct ggttactttt
    12361 gcaaacgatt caatataaat cagttttttt cccatcattt ttgttattaa gcagataggg
    12421 atcatagcta aaacacctgt acaaatcacg agatccggtt tttcctttat atatataata
    12481 attgatttga ataaattaat aagtatagta cacaaaaaaa acttttcttt tctatttact
    12541 tgtctcatgt agtacgtttt ttcgcctttt gactcagctg aatattctgt ctgttctgtt
    12601 ataacaaaac tctcatactt ctccatcagt ggttttaaca tcattaactg ttcataatgc
    12661 cccccagagg aagccgcaaa acatatcttt ttgttcatat taacctctct atcagtcacg
    12721 ctgaaaaata tctctagtgt aaactttttc tttaatatca tccaagcagg tatcatatcg
    12781 attggcaata atagaatcac acatcgcctt aaactgctct aaatcgttga taaccttact
    12841 tccgaaaaaa gtctctccat ctttcagtgc aggctcataa atcaccacag tcgcaccttt
    12901 agcttttacg cgcttcataa ctccctggat tgaactctga cgaaaattat cactgttgct
    12961 cttcatggtc agccggtata taccgattaa aactggattt tccctgttga catcatactg
    13021 attatattca gcgtagttgt aatagcccgc catctgaaga acacgatctg cgatgaaatc
    13081 cttacgggta cgattagatt caacgatagc ctcaatcaaa tttccaggaa catctgcata
    13141 gtttgccaac aactgcttgg tatcttttgg caagcaataa cctccatatc caaaggatgg
    13201 attgttgtag tgagttccaa tacgaggatc gagacaaaca ccattgatta tctgctgagt
    13261 attcagcccc ttcatttcag cataagtgtc taattcattg aaatagctga ctcgcagtgc
    13321 taaataggta tttgcaaaca gctttactgc ttcggcttca gtgaatccca taaaaagagt
    13381 atccacgttc tccttaattg cacttttctg cagaagtgtt gcaaaaacat gagcagcatt
    13441 taacaagcgc tcatcagtca tatcagtacc aacaataata cgagatggat acagattatc
    13501 atataaagcc ttgctctcgc gcagaaactc tggactaaaa ataatgttct tactccccgt
    13561 tgtctcacgg actgattcag tgtagccaac aggaattgta gacttgatga ccatgatggt
    13621 atcaggattc acgtccatca caacctcaat aactgactca acagcagaag tatcgaaatg
    13681 ctgagtacga ctgtcatagt tagtcggtgt agcaataaca acgaaatctg cggatttata
    13741 agcgctcctt gcatccaaag tagcagttaa gtcaagttct ttttctgtaa gatacttttc
    13801 aatatattca tcctgaattg gagacttccg tttattaatc agttcaatct tttcagggat
    13861 aatgtctacc gcggttacct ggtgatgctg cgccaatagt gtggcgatac tcaaaccaac
    13921 ataaccggtg ccggctactg cgatgttgta ctttttcttc acaatatctc ctctttcctc
    13981 tcttaagtat ggagaatgtt actcctacgg ctattcatca tgtcaaaata acacagctta
    14041 gaatgttgat aaaataattt tccagaatgt gataaaatct ctttcattta taaaccacac
    14101 atctaatttt atctattctc cacaagacat gtagagtatt ctttaataac aagatacgac
    14161 cttactaaat acttcaaaaa attatcatat ttaattcata aatataaatc ttcattttaa
    14221 atgtatgccc aaaaatttat ctgtaatgcg tcatcaaaat ctagattcca aatctttatc
    14281 aatttttatc cactcattct accactttag taatattttc tatttataac actttcgtcc
    14341 tacattgatc cccccctctg aagtacagtt tttactgtct taaataaaat tttaaaatct
    14401 aaccccatac tccaattttt tatatattcc gtgtcaagtt ttgctacttc ttcaaaatca
    14461 gtaatatcac ttctaccact tacctgccac attccagtaa ttcctggctt gatagcaatt
    14521 cttgcacgat gatgataatc atattcttca tactctgatt taaggcatgg tcgaaatcct
    14581 ataaggctca tctttcctgt tagaacgttg aaaaattgag ggaattcatc taaagacgtg
    14641 gatctaataa attgaccgat tcctgtcttt tttgttccat ctggaagtat tttatttccg
    14701 ataattcttg gatcaaactc catcttaaac ataagattat tgctcatctt attttctttc
    14761 attaattcta cttttctttc ttctgcatcc atatacatac ttctaaactt atacatcttg
    14821 aacggtttac cattctgacc tatacgttct tgtgcaaaga aaattgggcc tggtgaatta
    14881 atataaataa gtggagcaat aaagataaaa ataattcccg ttgcaataca tccaacaata
    14941 cctgcaagaa tatcaattgt tcgttttata aatgcctgtt gagttgtcat aacattgata
    15001 cttgttgtaa gtactgtata ttctcctata cgaccaatag attgttttcc caaagcacta
    15061 tctgatactt ttgcaagatt caaatgtaca gtaaggccca tttcagagca tcgttcaata
    15121 aattctttca tacacgaagt acttttattc aaaataataa agacttcatc tacccatccc
    15181 tgacataagt aatccgctgc atttttggaa tcagaaacaa ctgtcactcc ctcaacatct
    15241 tcaccaatca tgcttttatc tatgataatg acaccattta ttttatacat tttatagtta
    15301 tgctctttca cattacgaat aactacttca gcaatactac tagatgtaac tataataagt
    15361 gatttgttcc cctcattttt cattttatgt ttcaagtaat gtttccataa gaggcgagtt
    15421 atatatgtaa ttacaacgta taaacctccc atcaagaaaa gaacgactct agaataatca
    15481 tttccaccct gaacactaat gagatacaaa cttcctaaca gcaccaacat aatcgcttgc
    15541 tttacagaag aagcaaattc tatatagtac cctcttttta atacacctct atatgcttca
    15601 aacatgaaaa taactaaaag atctgccagt tcaatgaaga ctgccatgtt ttgatacatt
    15661 ggaatcacat aaggattcca atctacctgg cgtaagacat acgatagata aaaagcaagc
    15721 tggatacaca ataaatctaa aataataaaa tcaatatgtt taacccatcc ccttgaactt
    15781 ttcttgtaca ttccttttta ctcccctctt tttaataaaa taaattgtat tacttgtttt
    15841 ctctcctaaa tatctttctg aaggattatt gactcgacaa attggcttgt ggatattctt
    15901 atatatagaa catccgtaag ccaacttaaa actccttaac aaaatttttt tactcatatt
    15961 gtagatcaaa acaagtatcc aagaaatcat attttatttc ttttttctgc ttactcaaca
    16021 acagctaaaa gaaaaatcat cataaacaaa ttcaactaag ccaacgatcc ccttgtgaaa
    16081 tcaagttttt tcttgattta cgaataaaga gctaaaataa accaaacttc ttctttttta
    16141 cctcttcata gactggataa ctgatttcat ccccgttgat cagatccttt gcgacttgtt
    16201 tcatctgtac tactttttcc ttaccaaagt cctttgcaat ctgctcatag cactctttta
    16261 agtaaaaggt gcgcttagag accccatggg catcagaagc agccatctgc acgagattat
    16321 gtttgaccat cgttttggct gttttctgga tgtctttgcc aaatacccca acataacttg
    16381 gcgcagtgag ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt
    16441 cgcgaaaatg cacgttgcgt tctggatgga cgattactgg tgtctttcct agttctaaca
    16501 actcaaaaaa aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa
    16561 gatatgtatc atctaaatct gtaaataata tttcatctcg gtggatgtct tcgatgagcg
    16621 ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta
    16681 attcggcttg taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc
    16741 cattattgtg gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc gctttttcag
    16801 ccattgcaat acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc
    16861 aatgtagatc gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat
    16921 aaccagcatc tttgctatgt tctgctccgt tgtacacaac acctaagact cgtgcttgta
    16981 ccatctccag cagttctttt gctttagtca aggattcttt acgtgacata ttttcacgta
    17041 cgaccaataa agtgccatcc gtttttaaag acatgatctg tgcatctgtt acggctacta
    17101 ctggtggcat atcgaatatc accacgtcaa ataactgtct tgcttcggat aggatctgat
    17161 ccattcttgg cgagcttaac aattctgatg ggttgggtgg cttaggtcca ctaggcaaaa
    17221 tcgacaagtt ctcgactggc gttcgttgga tcacatctgc tacacttcct gatgaactaa
    17281 gtgctgtact taatccactg gcattgttca gctggaatgt cttatagacg acgggtttac
    17341 gtaaatctgc atcgactagt agaactcgtt gacctgattt agcaaaaacg actgcaatgt
    17401 ttgctgcagt ggtggatttc ccttctccag gacctgatga agtcacgaca atcgttttga
    17461 tttgctgacc agctgcggag gcaaactgga tattcgtccg gatcgtacga tattgttcgg
    17521 cgattggtga agatgggttt gttaatgtga tcaaactgac tgcatgagtt tgtgctgctt
    17581 tttgtttctg tgttcgtgcc atttttcttc ctcctagact cttgagcggc ttctgcgtga
    17641 tggggctgtt ttatcgtttg tagcttttgg tcgtgcagga gctgttcctt ttttacggat
    17701 accagcgttc aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa
    17761 tgtatctgtc acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc
    17821 cagacctaca cctaccatca ttcctaacat taagccagtc attaaattca atttattatt
    17881 tggcgaaaca ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt
    17941 catcacatct tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc
    18001 agccactgca ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt
    18061 aactgagatg gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa
    18121 acgatctttg acttcattga gcactaaatc actgacgatc atatctttat acgtattgat
    18181 catttgcagg ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac
    18241 gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga
    18301 gatggccaag ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc
    18361 aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc
    18421 tagattcatt tgatctttta attctttttt cacacgatca agttcttgat cactgacttt
    18481 ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt
    18541 accaaatgcc tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt
    18601 tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact
    18661 caatactttt ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc
    18721 accatttggg tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt
    18781 aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc
    18841 gataccacca actgcatcaa cgagttcctt tagtcctttc atattgatag agacatagtg
    18901 atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa
    18961 tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc
    19021 tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat
    19081 cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag
    19141 aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg
    19201 ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc
    19261 tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca aaaatatttt
    19321 tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt
    19381 gaatcaacgt tttaactgtc aatagcttat attgctatag cccatttgct gagcaacagt
    19441 ttgtgctttt gttgcgtatg cggctcttat tttttcattc cggatctgct ctatttgact
    19501 gagcgccgtc aaatattgat cgtaagtagc ggtagctgtt acttgaccgt ctttcagcat
    19561 cgtatcgaat agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag cgaatcctag
    19621 atattgcata acgactgttt tccaagcact atccttaaaa aagctcatat tttcacgtac
    19681 atgggcgaca tctgcacttg ccaacttttc tttgatgatg acatcatcca cagcttgctg
    19741 ccagtttgat acgtttttag taaataattt attgacagcc gtctggatct tcagttttga
    19801 ttctgcatcc tctaactgcg ttaagactgc tttcttttct tcttggatcg cagcagcttc
    19861 tttaggtaat tctgattcct tgatgtcata atcatctgcc gaaagtttga tagaagatag
    19921 tttatgtgac aaatttgaaa ctgtttcttc tgttatttca ggatttagga aaattttttc
    19981 ttttgtactg tacagcttat ctacttcttt tgataaagag gtcacggtgt ctttttgatt
    20041 ggtgatggca gattctgcgt aattgatccg ttgatgatat tgccgctgat tattgagatg
    20101 gtatccaaac aagcaaatca caatagcgcc tgtgcctacc gccacagtag ccaataactt
    20161 gacgaacggc ttttccatag attcactcct aaatattgat gatagataag taaagatgtg
    20221 attcattcac atcagaatga gcgcttttta ttttaaaagc ataattaaac taatatattc
    20281 atagaaatat ttataaacag gttttcccta tataatcata ccacatacag tttttttgac
    20341 tagggaaatt cctaatttaa aagtaaataa aacagtaaca agattataat taatcgtttt
    20401 catttttatt tcctcacttt tcatgagcgt tttgaaaatt cgaacaaaga ataaaataac
    20461 aaaaattatt aaaatagaat taataacagc tatttataac aaaaaaaaca gagacacagc
    20521 acgtttcgtg catgctctct gtttttcaaa caaaagacta ttttgtgatg atttgatcca
    20581 agcttgctaa tgacaaagcc atggcagcta tttgacttag ttgtgttagt cggttcttac
    20641 gtactgctgg ttcatcagcc atgaccatcg tttgatcaaa ataattttcg atcagtggac
    20701 gaagagcgac aagctggtcg tagttttcgt ggatcgtttg gtcagcaaaa cttgctttca
    20761 ttgcttctac tgctttgtgt aattctttct ctgcgtcgtt ttcgaacagc tcagggtcta
    20821 cttctgattg tttttcaagc tgcgcttttt tcgctaagtt cacgacacgt gtcaaagctt
    20881 ccattgaagg tttgaagtct tcatctgcaa gatgttgttt gaacaattgt gctgctgcaa
    20941 atacttttgt cagatctttt tgttccgcat tcaaaacagc atcgatgaca tcatgacgaa
    21001 tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acttgttgtt
    21061 gcccttctga taaaccaatg ccgaaacgat ccgtatcttt actgataaca tcgctaatcg
    21121 tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatc cgaacgacac
    21181 cgtatgcttg gcgacgtaag gcataaggat cgtttgaccc gcttggaatc atcccaactg
    21241 caaagaaaga aaagatgcta tctaatttat ctgcaatcgc taaaacagaa cctactgtag
    21301 actgaggtag ctctccttca ctggatatag gcatgtaatg ctcacgaatc gctgtcgcta
    21361 cagcaggttt ctcgcctttc agaagagcat atttttcgcc catgatccct tgtagttcag
    21421 gaaattcccc taccatgttg gttactagat caaatttata gatttcagca gctcgttgca
    21481 aatccatcaa ttcttcctca gatagtccta cttgttcgcc aatgatacgg gcaatgactt
    21541 gtacccgttg cattttctca tagatactac cgattttttc atggaaagta acagttttta
    21601 gtttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat
    21661 cttctaaacg agccgtcaat actttttgat tgcctcgaac cacatcttcc aattttaccc
    21721 gatcaccatt acgagcagaa ataaaatgag gaagcagcat accttgatca ttccgtacct
    21781 cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga acagaaagat
    21841 atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt
    21901 ctagtaaatc ttcgtctaga tcaataaccc agtgattttt ttctgctaat tcgtttgctt
    21961 gtgtaacgat catttcttgt cttttttgcg gagacgcaat gacgaattgt tcattcaatt
    22021 tttcttcata ttcttttgca tgcgcaaatg tcacatcttc gcctaagaaa cgatgacctc
    22081 gagaggctct gcctgttttg acatccaaga tttcgaatgg gattacttcg tcatctaaca
    22141 atgccacgat ccagtgaata gggcggatat attcaaaatc gtattctgcc caatgcatcg
    22201 ttacagggaa agtcaaactt ttgatcaccc ctgctaagcc tgtcaatact tcttttgctt
    22261 ccttaccatg agtgtatttc gttacataga catattccac accttttaat tctctaaaag
    22321 tgatagcgtc tgtcgtcaga ccttgtccgc ggacaaatcc ttccgctgct ttcgtccagt
    22381 ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct tcactgtccg
    22441 cttgtcgttc tggaatattt gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt
    22501 cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt
    22561 gtttcatgct tggtgtgaca acatgggcag gcatttcttc cagcccgatt tcaagtaata
    22621 agtcttttgc catcttattc agcctccttc acggtttctg gatttgctgt gtttttattc
    22681 aacaaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc
    22741 atgttgcgga tgcgagaaag ataaccggca cgctctgtta cggatactgc accgcgggcg
    22801 tctaataagt tgaatgtatg gctacatttc aagatataat catacgccgg atgaacaaga
    22861 ttttcatcga tacagcgttt agcttctttt tcaaaacgat caaagttgct tagtagcaag
    22921 tcttggtcgc ttacttcaaa agaatatttg gaatgttcat attctggttg tttgaagatc
    22981 tcgccatatt tcacgccatc agcccattct aaatcataga cactttcaac ttcttggata
    23041 taagaagcaa gacgttctaa tccataagtg atctcagaag ttactggttt acaaggcagt
    23101 ccaccaactt gctggaagta cgtgaattgt gtgatttcca ttccgtcaag ccatacttcc
    23161 caacccacac cggcacagcc catggatggg ttctcccagt tgtcttcaac aaaacggatg
    23221 tcgtgttcta atggatcgat tcctaaaagg cgcaagcttt ctaaataaag ctcttgaata
    23281 ttctcaggtg aaggtttcat caccacttgg aattgatgat gctgatacaa acggtttggg
    23341 ttttcaccat aacgtccatc agctggtcgg cgagatggct ctacgtaagc agcattccat
    23401 ggttctggtc cgattgcccg taaaaaggta tatgggctca tcgtacctgc tcctttttct
    23461 gtgtcataag cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa
    23521 atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa ttttaaagcg
    23581 ttgctgtcaa ataagaatac aggaaagaaa acgacaaaaa atccctatgt ctgattacag
    23641 acatagggac gaataattcg cggttccacc ctacttccga ataaagattc ggcagcttca
    23701 tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggctccc actctcccga
    23761 actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt
    23821 ttcttgtttc tataatagat cctatcactg aacgatctat ttaatctatg gttaataata
    23881 gacggaactc cctgtgtttg tcaaatggtt ttgatttttt gttgtatgtt ttatttacta
    23941 ttcttctttt tcaggtgtct ctttttcttt ttcagacatg tctttctctg acgtctttaa
    24001 tacgtgtgcc caagacttca tttgattgat gaactttttg cttttcaaat ggataccgac
    24061 atattcatca tacagctggt cgatgagttc acggattgct tgtttggttt cttctttcag
    24121 atggattcca ctaattttat cataagaaat agctgaaaac atccgaacga aataaacagc
    24181 tcgaggatct gcatgataac ggtgatcgtc catcgaccaa tgtctttcgc atatcacacc
    24241 accatatttg gaagaatagt caaattttcc ttttgtttcc ccgcatacga cgcagtgtgt
    24301 ccagattggt gcaattccga agcgttgcag tatttggact tcaaaaatat tcgtgatgat
    24361 ttctgcatcg tctccttggt ccatccgttg cagtgcttgt tgcgtaaact ggaacaaatg
    24421 cgggtcgtac tgatgatctt cgattgccgc atctactaga ttcaataaat atgtgccata
    24481 gccgctcaaa aagatgtctt cttggatgtg tcgaaacgca tggacatctt tgacactgct
    24541 taaaaatgac aaaccttcac ttcgaaaatt cccgatataa actgcttcag taaaagggag
    24601 caaagccgga gctaagggct gattttgccg atggatctct ttgatataaa acatcatttt
    24661 gccagaagat tctgtaaaga ttttgactaa tttatctttt tctttgtaat tacggctaga
    24721 taaaatcaag ccttttgatt ctgcaagctg gctcatattc tccctcctaa aaaataaatc
    24781 tggaaaagca gattctgccg atccagatca tttatgttgc gatcttccgc agcttttctc
    24841 aacacgcgga tttttattca gtttatttct atacctaatt tacaagaagc tcagaatatc
    24901 agtattcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct cgccagtctt
    24961 tttgaacttt gacccaaagt tctagaaaga ctttgtctcc taaaagattt tcgatatctt
    25021 ttcttgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata atgatccctt
    25081 tttggctatc tctttcgaca ataatcgtag cctggatatg gattttatca ttctcgtttc
    25141 gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca
    25201 agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca gtcacttgat
    25261 catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc atcaaacgtt
    25321 cgacgttgtt tccttctgta gcagagattg gaatcacttg tgcaaaaggc atctgcgttg
    25381 aataatcttc gatgattccc aacaactcgt ctggatggat tttgtcgatt ttattgataa
    25441 tcagataaac aggagaatgc acgtttttta gacgttcaat aataaaatcg tctccccgac
    25501 cgcgtttttg atcagcacta atcataaata agaccgcgtc cacttctttc aacgcactgt
    25561 aagctgcttc taccataaaa tctcctaaac gatgctttgg tttatggatt cctggtgtat
    25621 caataaaaat caactgagct tctggtgtag tgtatactcc ttggatttta tttctagttg
    25681 tctgtgcctt gtcactcatg atggcaattt tttgacccac gattcgattc aataatgtgg
    25741 atttccctac atttgggcgg ccgacgattg cgacaaaccc tgatttatgt tctggcatat
    25801 atgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata aaatcaaccc
    25861 aacgatgacg gtaaaacaag cggtcactaa aactgctcca gctgccatat ctttgatttt
    25921 tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc acgacatttt caaaaacagt
    25981 attgatgatc tctactatcc atacgagaaa aacagctaaa agcagccata accattctat
    26041 ccgatcaagt tggaaaatca cgcccataaa caaagcgagg attccgaacg caacatgttt
    26101 cctcatgttg cgttcttcct caaaaacagt tttgactcct tggacggcaa attccacgga
    26161 catcaggaaa tgtttgtttt tctctacgtt tttatctttt aagtccatag gcatctaaga
    26221 tctctttctg taaaccaaac atttctttct catcctctgg tgtcatatgg tcatacccgt
    26281 tgatatgcaa gaatccatga accgctaaaa agccaagttc ccgatcatac gaatggccgt
    26341 aagcttctgc ttgctctgca gcacgttcag tagagatcat gatgtctccc agtacacgag
    26401 gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgccc tcttcttcca
    26461 aggcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga ttgattactt
    26521 ggattgctgc attgtccatg aaagtaacag acatttctgt ttcttctggc aactctaaat
    26581 atgatgctgc aaattgcagc aagtcgtcga tttctttcat tttctcttct gagacttggt
    26641 ttgtttcatc catgaaggta atatccatta tacatcgctt ctttctattc ttcttgctat
    26701 tcttcttgtt cttcttttat cttctcaact tccgatttca ttttaggata tttgatccga
    26761 gaatggaagg tactgcttaa accgctgatc aatgattttt ctactttacg gatttctttt
    26821 aacgtcaatc cactgtcgtc caattgtcca tcggaaatac gctcttcaat aagattatgg
    26881 acaaaacttt cgattttttc gctcgttgga tggtccattg cccgtactgc cgcttcacaa
    26941 ctgtcagcta tattgacgat tcctgcttca cggctttgag gttttggtcc aggataacgg
    27001 aattgctctt ccgtaacttc cgggttgcgt tcctttgcct taacgtagaa ataacgcatc
    27061 agcgtggttc catgatgctg tctacaaata tcgatcacca tttgcggcat attataatct
    27121 tccaatattt tggcaccatc tgtcacatgt ccaaagataa tatgtttact gtcttctggc
    27181 aataagaaat tgtgcggatt ttccgctcct gaaggcaagt tctcaacgaa aaagctggcg
    27241 tgtttgatct tgccgatatc atgataatag caagctactc gagttagtaa agaacgtcca
    27301 ccgatttctg ctacagcatt agcacttaga ttggctacca tcatgctatg gtgatacgta
    27361 cctggtgctt cttccagcaa ctgtttcaat aaaggatgat tgggattgct gagttcattt
    27421 aatacgatca cactatcatc attgaccatc aattcaatat aggggtgcag acctatcgtc
    27481 agcaagaatg aaaacagtgc acctgtgaat ccgcagacta gcgttaacca agtcgttgca
    27541 tccccaaagc tcattccttg gtagataatc aaaatgacat ccatcatcac aggaaaaata
    27601 atgacccaca tagcagcgga taacccttgt tctgagatcc gtttgcgtct gacgatcgtt
    27661 gccatgagac cagaaaatac ataggacatc aaaataactg tcagaaagtt cgtgccaatc
    27721 gaatcataaa aaatgaaaag tgcggatact gcttgaaaaa taccagccat gatccctgct
    27781 cttcgattca agaaaaagct taaaacaagt ggagcaaaag ccgcggggta aaacagcgga
    27841 atataagcag cttgttctgt ttggaagagt tggaaaaatt tcattagtac gacgcttact
    27901 gacattgccg tcacgtagaa aagtacgtat tttgttcttt ctcctgcatt ttggaattga
    27961 agtgaattat aaatcagtac accgatttga agcaaaacag ctaaaaccat ggcaatgagt
    28021 gggaaaatag aagtcgtttg attcgtcaat cctagtaatt ccaatttttt catcgctgaa
    28081 gcatcaattt gatttccttc gcgaacaata atttctcctt ggaagatcat cactggttgt
    28141 acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa tacatcatta
    28201 actacgatgc cttgatccac taagtagcga atcatttgtt gctgctctgt tgtgacattc
    28261 aaatattgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct ttcacgaact
    28321 tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaactgt gtgaatttcc
    28381 gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg gtaaaacgca
    28441 acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg ctcttctgta
    28501 gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgccatt ataagatttg
    28561 ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg attgttctgg
    28621 tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc tgccagtttt
    28681 cttctttgtt cggtttcttc cgtgttctca atcgttttat tcgcacggat actctcttct
    28741 gctacttggc cttctctgaa atcaatggcc ttttgtttga cactactaaa tgtaacgccg
    28801 cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc gagtttttct
    28861 tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa gcttattctt
    28921 gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg tgccgaacaa
    28981 cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttaat gttcgttctg
    29041 catgaacaag accacttgtc gtcccacgag gcaagtctat ctgacttgta tccccattga
    29101 caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga gcgactgtcg
    29161 tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc cgcatgtaag
    29221 ctaaaggtgc gatttcgatc acgcctcgtt ccatcaatcg gttcgtatga tccaatccaa
    29281 aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt tctttcagat
    29341 ccccgggtag aaagcctagg ttttcccctg cctccacagc gggccgcgtc aaaatgattt
    29401 tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga aacgttttcc
    29461 ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat tcaacgtatt
    29521 tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg acgatctctt
    29581 cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccattttc aatgccgtca
    29641 caacatctgg cgtacttacg tggattcctc gtttgatcaa ttcctgtaat gtccgaatga
    29701 ctgaggcagc caaggaaatc tgggattgct caccaatgat ctgaatgatt tctccacgtg
    29761 tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg tgtgcaccta
    29821 aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta gactcttccg
    29881 tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca tactcagaag
    29941 aaactatata gcaaataaga aaaggttgta aaagagtcgc ttaaccatac gtaccaagaa
    30001 acgttaaaaa atagccgttt ccattcgcta aaggtctcgt tttgataaaa agaagctgag
    30061 aatgtaagtt atatgaaagt ggagaacaaa atatttcgtt tcattcaaca agtattctct
    30121 aggtattttt cctgtttcaa aaaaagaaaa caaaatagtt aaaatattct tgcatttcaa
    30181 aaaaaacagg tgtattatta cgtcaacaaa taaacataca gcaaagcgtc gaggaaaaca
    30241 gtaggctatt caacgattta ttcccagtga gtcatgtaag tgagaaatga caataaatcg
    30301 aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca
    30361 ttatcaggct agcggataac tttatcaaaa agcgtccgtg aatgaagaag cttttttaag
    30421 cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga aaagggcttt
    30481 tttttatttt aaggtggtgg ttttcatgga atctgatatt agtggcatta ctaattgctg
    30541 caacgtttat atcgtcaatc aaaatgataa ttattggagg aaaaatttat ggaagaaaca
    30601 aaaacttatt catggaaaca actattgatg gtcagttcac ttatcttcgg gatgttcttc
    30661 gggtcaggta atctaatctt tcctgtccac ttaggtcaaa tggctggcgc aaactgggtc
    30721 agtgctggcg taggatttgc tatctcaggt gctcttttcc cattactagc tatcttagct
    30781 gtagtagtga caaaaagtga tggtctgtat gacttagcta aacctgtcgg aaaatggtat
    30841 gctgccttgt tcttaatcct tgttcatttg acgatcggac catttttcgg tacaccacgg
    30901 acagctgcta ctgcttttga aatggctgct aaaccatttt tgcctgaaaa atataattca
    30961 ttaggtatgt ttttattctc tgctcttttc tttttacttg cttatttcct tactgtccat
    31021 ccaacacggt tggtcaaata cgtcggaaag tatctaaact ccttgtttct tgcattatta
    31081 gcaatcattt ttgtcgtttc tttattccat ccaatgggaa atctaaatca atctgctgca
    31141 gatacttatc aaacaaacgc tttaacaaat ggtgtcctag aaggttataa cacggttgat
    31201 gcagtagctt tgcttgcctt aagtgtcact tttgtccatg cagtccgtga ccttggtttc
    31261 aaagataaaa aagtcacaga attaacggca aaagctggta gcctgagcgt tgcgctagaa
    31321 gtcatgatct attttggact tgttcttctc ggtgcgatga gtttgaataa gatgtcttta
    31381 tcagaaaatg gcggaactgc actttctcag atcactaatt tttatttagg taacttcggc
    31441 gctagcttct taggagtgat ggtcacatta ggtgtcttca ctaccgctat gggactcgtt
    31501 gtttcttttg cgcaagattt ccataaactg ttccctaaag tcagttatat gacatggctg
    31561 cggttaacga ctttcgtctc ttttgtcgtt gcaaatgctg gtttagacaa tatcatccaa
    31621 tggtctttac cagtattgat gcttttgtat ccattgtcgc ttgcgttgat cttgctttcc
    31681 ttaacagcaa aatttttcca aaagacacca tttgtctatc aagtaacgat gttgtttgct
    31741 gctgttcctg ccgtattgga tatgctggca aattcgcctg ctttagtgag tcaacaaaga
    31801 gttgtagcaa gcatgctcga attttatcat catcacgttc cttttgctgc attaggactt
    31861 ggctggatgg tcccaacgtt acttggttac gctggcagcc tactgtttta ttacgcctat
    31921 cgcctatcag ggtataaaca agaagcaaat gaactaccag aagaataacc aaatcaaaca
    31981 caatttaaca taagaaatat taaacaggaa aaaaccagct agatgaaaga atcctattct
    32041 ttcgtctagc tattttttag ttctcttttt tacgcgcaca aaaaaactgc ggacaatgat
    32101 tgtccgcagt ttaaatgata aaattaagat tgtaatagtt ctttcacaat ggcatttact
    32161 tggtttccat ctgcttttcc tttgacttta ggcatgatcg cacccatgac tttgccaaat
    32221 tctttcattg atgaagcacc agtttgtgtg attgcttctt gaacaagttg tcgaatttct
    32281 tcgtcggaaa gttgttccgg catgtatttt tcgactatca caatttcact ttttactttt
    32341 tcagctaaat cgtcacgtcc tgcttcttca aactcatgca aagaatcacg tcgctgtttc
    32401 atttcgcgag acaacaccgt taattcttct tcttctgtca agtcgtgacc ttctttaatc
    32461 tgctcgtttt gtatagatga cttgatcatt cggatgactt gtaaagattc cttatctttc
    32521 gctttcatcg ctgttttcat gtcatcgttt aatctactta gtagtgacac actaccaact
    32581 ccaattcatc acagaactag aattttttgc gttttctagc tgcttcagat tttttcttgc
    32641 gtcttacact tggtttttcg tagaattcac gtttacgaga ttcttgtaaa gtacccgctt
    32701 ttgacacgga acgtttgaag cgacgaagag catcgtcaag tgattcgttt ttgcgaacga
    32761 ctgtttttga catgttaatt ccctccctcc gagcttaaaa agtaaccgta attgttattg
    32821 aaatatatct caaaaaacaa gactgtcctg taaatattat atctaatgct gttttgaccg
    32881 tcaaggaaat cttgcttttt tttaaaatta tcaaatgtta ttttatttaa atccttattg
    32941 acaattttca catcttccga aaatctcgaa tctatgccct tcgatcgtac atccaccgag
    33001 ctgttcttcg aaataattca ttggacacat atggatttct ttcgttgttc cgcacaccgt
    33061 acagataaag tgatggtgat ggccgatttc cccttgacag caatgaaaac gaaacttcat
    33121 ctctccattt aaatcggttt cttccagtaa atcgatttct gcaaaatcat ggagatttcg
    33181 ataaatcgta tcgtaactta ctcctttgaa ttcctgattc atatagtcaa aaacttcttt
    33241 agcggatacg tagcgattgc gcttcactaa ataagttaac aatgcttctc tttttttagt
    33301 atatttaaaa ccgttatttt tcaatgtttc tagtgattgt tcaatcaatg tctgtttttt
    33361 catgacttat tcctccaaat caaaatgatt acgaagtaca cattctagag tatggtataa
    33421 taaacataaa aaaaatgcaa gtgaggtttt atccatgtct tccgattcta cagaaatcat
    33481 tacttttttt gtcatctctg actctgctgg agaaactgcg acaaaattag cgcaagccac
    33541 tatggcccaa tatcctaccg ttgagttcaa tttattccgc agaacatttg tgacagatga
    33601 gaaaacattg atgcaggcac tgcaagatgc actaaaagaa aaagcccttg tcctacacac
    33661 attgatcaat caagaattga tcgagatgac gcgttctttt tgtgaagaac atcaattatt
    33721 tagttttgat gtgatgactc cgccgatatc agaaatcgag cgcttaactg gtatcaagcc
    33781 cattcggcaa cctggcgccc ttcatttatt gaatgagaac tatttcaaac ggatcaaagc
    33841 aatggaattt gctgtaaaat acgatgacgg gaaagatcct cgtgggtttc tagaagcaga
    33901 tgtcgttttg ctaggcgtat ccagaacttc caaaacgcct ttgagcttgt ttttagctaa
    33961 caaaaattta aaggttgcca atctaccttt gatcccacaa gcacatattc ccaaacagct
    34021 ttgggagatc gatcctaaaa aaatcgtggg actgacgaat aacccagata ttttaaacaa
    34081 tatccgtaaa gaacggatgc gctcttacgg attaaatcct gatactgctt attccgatat
    34141 cgaaaaaata cgagctgaac tcgattttgc gaatgatctt tatgaaaaat tgggctgcgt
    34201 agtaatcaat gtcgcttctt tatctatcga agaaacagct tcacttattt tgaatgcttt
    34261 agacctagaa gatcatagtt attatggaac tgaaaccacc gaagaaacag actaacagtt
    34321 tttcttgtct ttttcttaaa aaaactggac tgtgtcagcg aagaagattc gttgatacag
    34381 tccagtttgt ctctatttgc acctatcgtt attgatgttc cacatgtttg agcgtctggt
    34441 tcaaaatatc cagtacgtga gaatcatcaa gactatacaa aatcgctttt ccctcacgat
    34501 gggacttgac gacatggttt tctcttaata aacgcagttg atgcgataca gcagattgtt
    34561 ccatttctac tgcttgggtg attgctgtca cattttgttc cccgtctttc agatacagca
    34621 gaattttgag acgagtgggg tcactcaaca ttttgaatag ttggctgact ttctctactt
    34681 ttgttttttg cgtgtctggc aatccattca cctccaactt gaaaaaagac tgacttctaa
    34741 agtttaccac tttttccatc agtctcaagt ttttttattc ttctgtcatt tttttgataa
    34801 tcgcttcata agcggctact ttttcagctg cgtcttctga agagtttcct tttacaccga
    34861 tataaaattt gattttcggc tcagttcctg aaggacggat agcgatccag ccattttctt
    34921 ccagcgtata cttcaatacg tcagatggcg gtgtcgttag tttttctacg tttccttcac
    34981 ggtctgttct cgtcaactct ttgaagtctt ctgtttggat gacttttgct ccggcaaatt
    35041 ctgtcggtgc ttcttcacgg aatttttcca tcaaagcaga gatttttgca gagccttctt
    35101 gtccgctcat tgtcacagaa atcgtttttt ctgcgtagta gccatatgtt tcaaaaatcg
    35161 tttgcaagcc gtcatacaat gttttgcctt gttttttata gtaagctgct acttctgcta
    35221 ataagagcaa tgcttgaatc gcgtctttgt cgcgaacgaa aggtttaact aaataaccgt
    35281 aactttcttc gaatccgaac ataaaagtat gggaatgatc ttcttcaaat tgttgaattt
    35341 tttcagcaat gaatttgaat cctgtcaata cattgaacat ggtagcccca tagtgttccg
    35401 caattgctgt aggcaattcg ctagaaacga ttgatttcaa taccgctgca ttttcaggta
    35461 aagtgcctcg cgattggtgt gcttccaaaa tatattgagc aaacagggct ccgatttggt
    35521 ttcctgtcaa tacttcatag gagccatttg gcaaacgaac ggctgcgcct aaacggtctg
    35581 catcaggatc cgttgcaatc agtaagtcag cgccttcttt ctcgccaagt cgcatcgcat
    35641 actcgaaagc agagtgttct tctggattcg gtgatttgac tgtagtgaaa tccgggtctg
    35701 cgacagcttg ttctggaact agtacgaatt gttcgaatcc agcttgtttc aacgcacgtt
    35761 cgcccaacat ttttcctgtt ccgtgaagtg gtgtgtacac aagcttcaaa tctttaccca
    35821 ttgtttccac caattcatga tcaatcgtta ctgctttgat ttcttctaga taagcggcat
    35881 ccacttcttc tccgatgatc gtgatcaatc cgctatgttc agcttcgtcg tcagataata
    35941 cttcaacttt taacggattg ctgacttcac ggacatattt cgtcaatgca tctgcgtctg
    36001 caggaggcat ttgtccccca tcttctccgt agactttata tccgttgtaa gctgctggat
    36061 tgtgggatgc agttaccatg attcccgcaa acgtcttcaa atgacggact gcaaaagaaa
    36121 gctctggtgt tggacgtaaa ctttcgaata cataagaagg gatattgtga tgtgctaatg
    36181 ttttggctgc ttccatggca aattctggtg attgatggcg tgagtcataa gcgatagcga
    36241 cacctcggcg ttttgtttct tcaccttgtg tatccatgaa acgtgcaagt ccttctgttg
    36301 cttgtcttac cgtatagatg ttcattcgat tgatcccagg tcctaagatt ccccgcatgc
    36361 ctgcagtacc gaattccaac ggtgcataaa aagcatcttc aagtttttca ggatcttcac
    36421 ccagatcatt taattgtttt tttaagtttt cttctaaatt ttcttcatgt acccattgtt
    36481 catacacttg ttcccaagac atgaaaataa cacctctttc tgaaatatgg ttcttaataa
    36541 gtatagcatt cacttattag atatgaaagt atgaggacca aaaaacgctt aaatcaagaa
    36601 aagattcttt tatcaagcac ggttacaagt attcgcataa atcggattca gtagatttca
    36661 cgcaaaaaaa agagtaaaag aacaattgaa accgttcttt cactcttttt aaacttatgc
    36721 agttgtttct tctaaagatt ccaagtattt atacacttgt tgtccagcaa tcccaccatc
    36781 accaacagca gtagtgattt gtcgcaaatc tttttcacga acatctccga tggcaaaaac
    36841 gcctggtata ttcgtcttca tttcgttgtc tgttggaatc cagccggctt cgttcgtcaa
    36901 cccagcttct ctaaatggtg cagttaatgg atccaatcct acatagatga atactccgtc
    36961 tgctgccaaa tcaaactcat tgccagtttt gacattacgt gctttcacac ctgtcactac
    37021 catttcattt ccaacgattt cgtcaacgac tgtatcccac ataaaggaaa ttttttcatt
    37081 cgcaaatgca cggtcttgga tgatcttttg tgcacgtaat tcatctctac ggtggacgat
    37141 gactacttca gaagcaaatt gtgtcaaata gatggcttct tctactgctg agtctcctcc
    37201 tccgatcact actagacgtt tgttacggaa aaatgcacca tcacaaactg cacagtaaga
    37261 aacgccacgt ccagcaaatt cttcctcgcc aggtacgcct aatttacggt ggatacagcc
    37321 tgttgcgata atgactgttt ttccttcgta agttttatct tcacaaataa cttctttgtg
    37381 gctgccgtga tccttgatat cttgcacgat tccataagca ttttctgtcc caaatcttga
    37441 aacaccttca tacatcttat aagctagatc aggacccatg attgaatcga aaccaggata
    37501 gttttcgatt tctgccgtat tgttcatttg gccgccagga gcgccttgtt cgatcatcaa
    37561 tacggataag tttgaacgtg aagcatataa agcagcagtc atcccagcag gccctgcgcc
    37621 aataacgatt acatcataca tatgtagatt ctcctttact taatcaagta agcatacttt
    37681 acagccaatg cgaaaaaaag tctattgttc tgcttgcgtt tctcgaatag ctttattata
    37741 ctcgaaaaat ccgctgaaaa acaatagact tttataaatc attgatcaat cgttcgaaag
    37801 attttctcca tttgaatcaa ttactttttt ataccagttg aatgatttct tcttcgaacg
    37861 ggctaatgtg ccatttcctt cgttgtcacg atcgacataa atgaatccgt accttttctt
    37921 catctcacct gtaccagctg atactaaatc aatacatccc caagtcgtat agcctaatag
    37981 atcgactcca tctaattcta ctgcatcttt cattgcctgg atatgtgctg ccagatagtc
    38041 gattcgataa tcatcttcca cataaccatt ctcatcgggc gtatcgactg cacctaagcc
    38101 gttttctaca ataaatagtg gtttttgata acgatcataa atatcattca tcgttattct
    38161 caagcctaat ggatcaattt gccaacccca ttcacttgct tccagatatg gatttttgac
    38221 agaagcaaag atatttcctg cagtctgttc caagagttcg ttgtcagtag tcgaaactcg
    38281 agaagaataa taagaaaagg agataaaatc aacagtatgt tctttcagca atgcggcatc
    38341 cccaggttcc attttgatct gaatgccatt tcgttccatt tctttcaatg cataagcggg
    38401 atattctcct cgtgactgga catcaatgaa gaaataattt tctcgatctg actttctaga
    38461 agcccaaaca tcttcaggat tgcaagtgta tggataatat gctcctgctg ccagcataca
    38521 gcctactttg ttctccggat ctacttcatg agcgatcttc gttgcgatgg cacttgcaac
    38581 caactcatga tgtgccgctt gatatttgat ttgttcttca ttttctcctt cttcaaaata
    38641 cagccctgcg cccatgaatg gtgcatgaag gatcatattg atctcattga atgtcaacca
    38701 atatttgaca agtcctttat agcgattgaa gatgactcgg cacagatttt cataaaaacc
    38761 aacgagttct ctggaacgcc acgcaccata ttctttgatc aaatgcatcg gacaatcaaa
    38821 atgtgtaatc gtcacaagcg gctcgatccc gtacttttga cattctttaa agacatcctc
    38881 ataaaatttc aacccagatt catttggctc tttttcatcg cccattggaa aaatacgact
    38941 ccaagcaatg gacatccgat aagtcttgaa ccccatttct gcaaataaag caatatcttc
    39001 cttataatga tgatacatat cgatcgcaat ttttgctgga taaaaatgat tttcatcaaa
    39061 ttcaaacatt ttcttttttc cagtaatgac aggaaaacgg tcttctccga ttggcacaac
    39121 atctacgttc gctaacccac gtccgccttc atcataaccg ccctcgcatt gattggctgc
    39181 tgtagctccg ccccataaaa aatcttttct gaatcccatc atacttcctc cattacatgt
    39241 gtattgcttg ataattctct tgccagttca tcttggactt cgattccttc tgcttttaac
    39301 cggctgattg tttccttgaa ttgcggtaaa tactgctgat gtgcaataaa caactcatcc
    39361 atgatccgtt ttgctgtctt tcctgatcgt ataagcggat tgatcgtaaa cgcttgcaaa
    39421 gctgttccat aatcaccagt cacggctgct tcgatcgtca gcaattccat attcttcatt
    39481 acttgcaacc agcctctttc agcagcagga agggaaccaa acgctacatt tcgtgcgcct
    39541 tgtgcgccaa cataggcagt gacttctact acacaatctg ccggcagatc aggaacagct
    39601 ccatcattct ttgtagatac cacgatctgt gtttctttat ttccatagat cgaagcaatc
    39661 gtttcacagg cagcatctga ataataagca ccacctcgtt tttgcaattg ttcaggttta
    39721 tgatccaatt ctggatcttt gtataattca aacagctctg cttctgtttc tttcacttgc
    39781 tgcgctcttg taccaatcgt ttgatattct tctaaagcat gttccagcat ttcttcttca
    39841 cgatagtaat agcggtggta gccacaaggg atcatgttca tttgttttaa ttgttctttg
    39901 aaaaacggga tgtcaaagat atttttcggc aatccattgt cttcatcgta taaacgatca
    39961 atcaattctg ccgtgcggtc atttccttta ttatctgcta ctttgtgcca atggaagtga
    40021 tttagaccgg caaacttgta gatcagctct tcttttttct tgccaagcat tgttggttct
    40081 gtcatcatcg ccattactgg cacattgcat aatccgatca ctttatccca tttgccatat
    40141 ctgataactg cttctgtcac catcccgctt gggttcgcga aattaatcag ccaagcattt
    40201 ggacataaac gcttcatgtc ttcaacgatt gccaaaacca ctggaacggt acgaaaagct
    40261 ttgaatatcc cgccagcacc atttgtttct tgcccaatca tcccataata actagggatc
    40321 cgctcgtctt tgattcgtgc atctaattga ccgacacgaa actgagtcgt aacaaaatcc
    40381 gcatctttca atgcttcttc ccgatccaga gttgcataga ttttgacttc atacggagaa
    40441 gcatcccaca tacgctgaga catctgactg acgatctcaa gcttttcttt tccttcttct
    40501 acatctacga gccagatttc tcgtatcggt aattcctcat atcttttgat aaatccttcc
    40561 atcaactcgg gggtgtaact gctgcctcca ccaatcgtag cgattttgat tccttttgcc
    40621 atgttctcac tcctttactt tttacacttt tattatcttt tttgtaaaca aaacaaacaa
    40681 tagaaattcg tttctttcca gtaacattta caaaagttag tgttttcttc tggatttttg
    40741 taaatatctt gtcaaaaaat gtctttatta agctatataa cataaatccg tttacaaatc
    40801 attttcaaaa aagttataat ttacgtaaaa aaacaagaag aaggtatcta catgttatta
    40861 cgtgaaaaaa tcaaagaaat ctccttttct ccttcagaaa atgaagtgat tcagtttctc
    40921 ttaagcgatt ctccagcact tggcgaaatg accatccaag agattgctga gcgatgttat
    40981 gtccacccgt ccacactcat ccgtgtagca aaaaaaatgg gttttgatgg ctgggtcagt
    41041 tttcgtaatg cttttttagc tgaaacagcc tatcttgaag ggaattttca agacgtagat
    41101 gcgaatcttc ctttttccac cacagacggt attatgacga tcgcaaataa aatggcttct
    41161 ttagaacaga tgacgattca agatacactt tctctcattc atcatgacca actgcaaaaa
    41221 gccaaacaac taatgcttca agcaaaaaaa atcgtgattt tcgccagtaa ctcgaatact
    41281 ttgatttctc aagatttcat gctgaaaatg aagcgcatca aaatggatgt cacgattgtc
    41341 accacttttg gtgaatcgtt ctatgaagcg tacaattgca caagtgatat gtgtgggatt
    41401 ctgatttctt atacgggtga aaacaatgtg atccttcgta ccgcagaaat attgaaacaa
    41461 aatcatgtgc caattttagc tttgaccagt atcggtgatt caacgctatc ttcaatgagt
    41521 acagccgctt tgcgacttac gacacgcgaa cgactgtatt cgaaaattgg taattttacg
    41581 atcaatacat cgatttgtta tcttctagac gtgttatata gttgtgtttt tgcagaaaat
    41641 tatcaacaga atttagtcca tctaatttct gtaggacaaa aatttgataa acgccctatc
    41701 agctcagcca tcatgaaaga gcctttcaat tttgaaaata aagaagtgga ctaaaaagaa
    41761 tagttaagcc tagtccattc tttcacctaa ccgttcaggt cagtgcggtg ataagtgcat
    41821 cgtctggccc gaccgtttcc ccttgatttt ccaaaatatc caaataatcc atgtaattga
    41881 tgacaatcac aggtgtttct aaactgtatc cttcctcttc gattttcttt ttgtcgaagc
    41941 gaagcaacag atcgccttgt ttcactcttt ctccttgctg caccaagctt tcaaaatatt
    42001 tcccttccaa ctgaacagta tccaatccga catgaatcag caattccaga cctgcttcag
    42061 aaaccatccc gattgcatgt ttcgtcggaa acagggacat gactgttccg tcaaaagggg
    42121 cacgtacttc tcctttttca gggtagatca agattcctcg acctagtgtt ccttgtgcaa
    42181 aagctgcatc ctttgctgca ttcagtggag cgatttgtcc ttgtatcggt gtttttacaa
    42241 tttctttacc tgatcttttc tgtctatcat ctgtttcatc tgcttcctcc ttccaaaaga
    42301 aaaaagtcag cgcaaaggat accaccatcg caatggcgat cccaatcaat acattaatca
    42361 catctgttaa atcaccatct ggtctaatat agttgggaat tgtaaagatg cctagtccac
    42421 cttgattcca agaagttaaa ttcattaacc cactatacgc gccccctaac gcaccaccga
    42481 cacaggtaaa aataaatatt tttttacgtg gcaatgtaaa cccataaata gctggctctg
    42541 taatccccac aataccagaa acaatggctg gtattgcaat tgctttgtct tttggatttc
    42601 taagcttaaa gtagagtgcg gtaagtgctg cagtttgtcc aaaagacact actgcaacag
    42661 gtgtcataat attttgccaa ccattcacag aaaattgcat caatcccatt ggtacgattg
    42721 cccaatggag accaaacatc accagaattt gccagaagaa gcctacgatt gctccataaa
    42781 taactggcga aagtgctaaa agtgtttcaa atccagccat taaaatatca gttgcgaatg
    42841 tgaatactgg tccaacgagt aaaaaaccta aaggcattgt aataagaacg gtgaagaatg
    42901 gaacgaagaa attcgcgatc atctcaggta ctaacttttt aattggtttc tgaatttggc
    42961 tagctaacaa cacaataaag ataatgggaa tgacagaaga agcgtagtta cgcgcaatcc
    43021 agggaatgcc gaatactgtt tgataaatag gtgcttcaaa aatagtgcca gaaaatatgg
    43081 tcgttagtgg tgcttctgct gcaccactca aagcttccag cgacaagcta ggattcatta
    43141 atgctgcgcc aagcaacatc cctacgaatt catttacttt aaatttacgg gcagctgtta
    43201 aaccgataat aacaggcata aattggaata cagaatcacc gatattatca aacataacaa
    43261 atgtgccaga accgcggtca aacgcgccta agaagacaag gaatgctgtc agtccacgaa
    43321 tcatcccgtc agcagctagg atagcaagga atggttggaa acagcccgat attccatcaa
    43381 taagcatatc aaacggtctc attttttctg ttgaaacagc ctcatcaaaa gtaaggtttc
    43441 ctgcttttat aacctcttca aacacgactg gcacatgatt cccgatgaca acttgatact
    43501 gccctcccgc tcgcattacc gttacaatac cgtccatatt tttcaagatc tcatcatttg
    43561 ctttttcttc gttttttaat ttgaaacgaa gccttgtgat acaatgtgtc aaaccattga
    43621 tattttcctt cccgccgaca ttttctacga tttttctcgc taattcttgg tattttccca
    43681 tttctattcc ctccaattta ttagtggaag gtttagccaa ctgaatgtca ctacccttat
    43741 ttcatacgtt cttgttaatt tatcctcctt tttttattta tttacaacga ttctcatgca
    43801 ctaaacgcgc agtatggatc gttaaataca ttttttcgtc cgtggacatc tggtagttga
    43861 acgtctcggc taaaaagtct gctattttct caacgcaacc aaacgtatcc ttatattttt
    43921 tttgaataac ctgaaacaat tcctcagaaa cttctcccac ttctgatttt tctccatcta
    43981 ggacgcgtgt cagaaaaaaa cgtaagtgtg tgacgaatcg aaaaaaatgg actgaatctt
    44041 catcaaatgt aatcttatag taatagcgga cgatatttag aatatcctgt attgttatag
    44101 tgaacaggta ggtattgtct tgttggcttt gaatgccttg tgcattgact aaatgcaatg
    44161 cgataaatcc ggcttcatct tctgataact tgatgtggta tttatccgag attttcttca
    44221 ctgttttttt tcccgatggc atactcttta ggaaatagtt gtttgatatc atacagcaat
    44281 acatttggta caaatattcc cattttgtga cgttcgatag ccgtatacaa atgatccgtc
    44341 aatgcaatat acacagaatc attcaactgg acttgtagag cctcttttgt atattcaatg
    44401 atttcacaag aaatttctaa gtactcaaga ggaatcgctt gtgccaattc ttggaactgt
    44461 tgtgaaagcc cgtcatttgt caactgaaat tttttgtcga tgcatgagtc aaaaatcggg
    44521 tcacccactt tctttttgaa tgccaaacct tttcccatca cgatcatttc ctgatttctt
    44581 tcatccctgg ttattacgac gttgttattc agtactttct caatcagcat gttttgctcc
    44641 tttccatgat atatagggct gtatccaaaa caaaaagaaa aacccaaaaa gccgtccatg
    44701 gaaccatggg agactcatca ggtttagctt gcatcaggca atcactatcc aagttgtatc
    44761 gttttcttta tattatcgaa agcaaacgct tttgtcaacg ctttcttagt ggttattagc
    44821 cgcttttgac tagctgtcgt cgtctttttt aaatcgggtg actacgccac taacgctcac
    44881 agatgaagaa tctgcgaagc tctgttttat tttcactcta gcgcttaggg caaaatgcta
    44941 ctatgctact gtcacaacct ctttttacac accactgctt tgcagtttgt acatatctgc
    45001 atacaaacca ttttgcgcta gaagtgtttc atgattccca cgctctacta tatgaccttt
    45061 gtctaaaact aaaatcaatt ctgcatcccg aatcgtggac agccgatggg caatcgctat
    45121 cgttgtccgt ccttgtcgca tttttgccaa tccttcttgg atcaatcctt cggtttcagt
    45181 atcgatattt gctgttgctt catccaatac tagtatttta ggatctgtca cgattgttcg
    45241 cgcaaaagtg atcagctgtc gttgtccact agaatagctg gcaccgtgtt cgatcacttt
    45301 tgagtgatat ccttttggta attcatcgat gaacttgttt gcttgaacaa actcagctgc
    45361 atggcgaatc tgttcgtctg tgatagctgg attcatcatc cggatatttc ctgcaatatc
    45421 tccataaaac aagaaggcat cttgcaagac caatcccatt ttcttacgta actcttccat
    45481 tggatattct cgaatgtcat gatcatcaat caagatttgg ccttcataaa attcgtaaaa
    45541 ccgcatcaat acgttgatga tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac
    45601 ggtctcaccc ggattagcga caaaagagat attgttcaat acattgtgct ctccatcata
    45661 agaaaagctt acattgcgaa actcgatctt acctgaagta atcgcttgat ctgctccagg
    45721 attttgttgc ggtgcatatt cttctgtatc catgattttc aaaatacgac taccggcaac
    45781 catcccatct gtaaaggtgc tcaaaaagtc catcatttga gacattggat taaaaaattc
    45841 ctggacatag gtcgtaaatg catagatcag tccaacttct acgggagaat gcaacgcatc
    45901 gatcccaaac aaagtcaacg ccaacgcaat cgccaatgca tagagaaaat tgatgattgg
    45961 acttaacaga atcgaattcg ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt
    46021 cacttgttca aattctttag ctaaacgagc ttcttggcga aactgctgga taatacgcat
    46081 gccggaaatc gattcattca atttcgtgtt cagctgactt aatttttccc gcatatttcg
    46141 gtaaatacga gaactgaatt tctggtaata ccagatgacg attcctaaaa tcggcaagaa
    46201 caccaaagtc cacaaggcaa tttctccatt gatttgaaac atggcaacga aagaagaaac
    46261 gactgcaaag atcccagtca ataccatcaa aaagacatac caaaattcaa atagcgtttc
    46321 tgtgtcattc gtcactcgag aaacggtcga tcctgccggt gtctggtcaa aataacgcat
    46381 acctagtgta tgcagctttt caaataatct tacccgaaca tactgatagg tcttcaatga
    46441 agccatggaa tacaaatacc attggaaaaa ccaaatgata cttttgacta tgactccaaa
    46501 caagtacaca ccagcaaaaa acaaaataac ttgtgtcgta gctgactgtt tagccagatg
    46561 atcatccata aaagtttgga tgatccttgg caataggaca ttgatcacag acagggcaaa
    46621 cgcaaataag atagcaacga tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg
    46681 tttgatgatc ttcttctgtt ctttaagtgg aatcgatttt gtccattccg attgataatt
    46741 ttcatccatc aggcctcgcc tccttctatc ttcgcttcta attgctgttt ttcccacatt
    46801 cgttcatacc agccacctaa tgcaagcagc tcgtggtggg ttcctcgttc aatgattttc
    46861 ccttgatcga caacaatgat ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca
    46921 atgatggttg ttttctcttt tcttatttct tttaaattat gcaagatagc ttcttctgtt
    46981 ttggcatcta ctgcagaaag tgcatcatct aagatcagca attcaggatt tttgatcaat
    47041 gctcgagcaa tcgaaatcct ctgtttctgt ccgccagata aagagactcc acgttctcca
    47101 actaaggtat cataaccttt tggcaattcc ttgatttcat tgttgataaa tgctaaagaa
    47161 gccgcatgct ctacttcttc ttctgaaagt tttggattag aaaaacggat attatctcga
    47221 atggtcatcg aaaacaggaa gtgatcctga ggcacataac cgattgatcc taataaagca
    47281 ttcaatgaat attctttgat cgaacgtccg ccaaattgga ttttcccttg ataatcatcg
    47341 tattcacgca tcagtaattt gatgattgtc gtttttcccg caccagtttt cccaacaata
    47401 cctaatgttt ctccttcttg gatcgcaaat ttgatttgtt ctaatgctgg tgattcttct
    47461 ttcggatagt taaaagagga aatagctaca ttcagctcgc cttttgccgg ggtctgcact
    47521 gcacccagtt gttccacgat atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa
    47581 gaagcatttc ctctttccaa tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca
    47641 agcataccga tatagctaat aaatgagacc atctgcccga ttgagatcgt gccttccaag
    47701 acatactttc cacctaaaat aattgttgca acataagaca gaccgataat caaggtaatg
    47761 aatggatcaa ataaggagtc taaaaaattg actcgtttgt ttttaataat tgcatcttca
    47821 atcttttctt caaaatccgc gagatcttct ttttcttgtc caaatgtttt aatgactttg
    47881 atcccagtga tactttcttg tgtcttatca ttgatactag aaaaagcttc ttgcgaatca
    47941 cgaaaagcat cgtggagttt tgaccccagc actcgtgaag taacggctag caaaggcaga
    48001 ggaatcagcg caatcagtgt taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt
    48061 gttccgcccg taatcactga atctgcaaag gtcaagattc cagctcctgc tacattctgg
    48121 attgcattca aatcattcgt tgcatgagcc atcaaatctc cagtccgata tttttggtaa
    48181 aagagattgt ccatttctgt aaaatgtttg aatagctgct gccgaagttc tctctccaac
    48241 cgagctgcac ttccccaaat attcgtccgc cagatataac gaaaaagata ttgtaagaaa
    48301 gctgcaagga tcaataataa gatccaccag gaaatgatct ttattgataa attcttatcg
    48361 gcgatctcat caatgatgat cccaatcact tttggaggaa ctaactgtac caaggccact
    48421 gcgaataggg agaaaatccc aattaaataa tgtttctttt cctgacgaaa aaaccaacct
    48481 aattttctaa aaatcgacat tttactcctc ctttgtcttt atcaagagca taggattgtc
    48541 tgtgcttgtc ttttttgctg aatagtctga atagtctaac aaattacgaa ataaaaaacg
    48601 gactgaaaaa atcagcccgc aaaaagtctt tgggtcagaa cctatttttt cccttggttc
    48661 ttcatttgct gcatcatctg gcgaattttc ttctcagatg gtttttgccc cattgacatc
    48721 atcattgatc gcaacatgtc ctcattaacg ggaggatttt tttctaaata gtctttcata
    48781 tatttacgcg ctaggaaaaa gccacctact gcgccgatta gcaaagcaat aatagcaata
    48841 agtacgacaa tacctgttga taccatataa atctctcctt tctatttccg aattgctctc
    48901 aatgatttat tttactaaag tttattgaaa aaagaaaggc gtattcttta gtttcatgca
    48961 aaaaatagtg gtttttttag agaagaacaa aatttctttc attaaccagc aagacacacc
    49021 acgaaaaaaa aattaggcat accgaatctt tttctttaat tttttctttc tgaggtgtag
    49081 aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat ccaacaaaca
    49141 atttcataag catcgactta ttgaacatac aaatggttta tctttagctg aagtaaatgg
    49201 aacagtggat gtaccaaagg gaaaaagttt ttttaaaaca cttcttgctt actctggacc
    49261 aggtgcatta gtagcagttg gttatatgga tccaggtaac tggtccactt cgatcactgg
    49321 tggacaaaat ttccaatatt tattgatgtc agttattttg atgtctagtc taattgctat
    49381 gcttttacag tatatggcag caaaattagg aatcgtgacc caaatggatt tggcccaagc
    49441 aatccgagca cggacaagta agacattggg tgtcatttta tggatcttga ctgaattagc
    49501 cattatggca acagacatcg cagaagtcat tggtgcagca atcgccctct acttactttt
    49561 tgatattcca ttggtgattg ccgtctttat tactgtatta gatgttttcg ttctcttact
    49621 actgacaaaa gtcgggtttc gtaagatcga agcactggtt gtctgcttaa ttttcgtcat
    49681 tttattcgtt tttgtctatc aagttggctt gtctaaccca gattggcaca gtttatttgc
    49741 tggctttatc cctaacagtc atacattctc agactcaccg gtcatcggcg gcgaaactcc
    49801 gctgactgga gctttaggga ttattggtgc aacagtcatg ccgcataact tatatcttca
    49861 ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac gaagaagatg tagcacaagc
    49921 tgtacgtttt tctacttggg attctaatat ccagttgtca atggcttttc tagtcaatgc
    49981 actgctattg atcatgggtg tcgctgtctt caaaaccggt tctgtccaag atccttcctt
    50041 ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta ttttgattgc
    50101 tgtagcaaaa tctggtatct tgtccacttt atttgctgta gcacttcttg cttctggcca
    50161 gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggat tcatccatat
    50221 gcgtatcccg atttggctaa gaagactggt cacacgattg ctttccgtga ttcctgtatt
    50281 gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacatgccg ctttgaataa
    50341 cttaatgaat gaatcccagg tgttcttggc ttttgcgttg ccattttcga tgattccttt
    50401 actgatgatg accaacagcc aagcggagat gggtagccga tttaaaaatt cattcatcgt
    50461 aaaacttctt ggctggttct ctgtgattag tctaacttac ttgaacttac gaggattacc
    50521 aggacagatc gaaagtttct tcggcgagca agccacttcc gctcaactag cattggctga
    50581 tcacatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga cgattggtga
    50641 tttatataaa ggaaacaaaa aatatgaaca gcttttgcag gaaacggtag aagaaatcga
    50701 ataaaaactg atttttccac acaaagaccc ttgatagtta ggttaaaaat ctagctacca
    50761 agggtctttt tatttagaag ataaagcttt tttcagattt aatttactga aaactagcat
    50821 gcttgttact acgatgaatt gcgttaatgg cacgcctgca ccagtgtaga cacttaaaaa
    50881 atagtccttt ttttctggcg cataagaaca aaaccatgag acaccgaaaa gaaaaaccaa
    50941 ttgtgtcagt attaatccgg tgaccacaat caaagaggaa ctataatagg cacgatcagc
    51001 ttttgcaaat acggttccta gtatcataag taatatccct gctattgaag caatcagcac
    51061 gggaatagcc agaacttggt aaatttttat gatgacattc gcgacttttg ctgattttgt
    51121 taattggaaa ttaggtgttc tcggttcatt actatcgatc caattctcca ttagcggaag
    51181 gtttaataat tcttgagcct gtttgacttg ttcttgcgtt ccgaaactat aatttcctcc
    51241 ttgatggaaa tctttatatc gccatgcata agcgtatcct gattgaagga aatcgaatac
    51301 gattggcaga tcactcatcg tctttccatc tcctgatccc atcagataga actctttttt
    51361 ctttttgatg gttcccttgt gataggcttc ttctaattct tggttgactt ttttccaaaa
    51421 atcattggtt tcttttgcat tgttttggta ataaccagat tcgcttacca catctcgcaa
    51481 tgcccaaaag atgatatctc cgggtaattc ttttactttt ccaccttgag tccacgcgtg
    51541 agtcgtatat aattcgtcaa tacgggtaga aacagtatgg aatgtaggag atgcggctaa
    51601 tgctttattt agtgcagcat gtgagaccca tatctcactg ttttctttat tcaagttcgt
    51661 ttcatcatct attcggatca gttgtttcat caaacgtcca aattctccac tggaacggtc
    51721 attgatggtt gcaataccgt atgttgtctc atttttatat tcgatccact gatagacgat
    51781 ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc agccattccc gtttcgagag
    51841 aagaaagagt cgttttttta acttctttac ttctttgaaa gaaaagtctt gccctagcaa
    51901 gatctggacg aaactgatac ccaaagctac tacagtaaaa ggaagaagcc agatagaatc
    51961 ttcgcgaatg aaccaaaaga aaggaaaagc gacagaaagc cctaaaaacc atggcagcaa
    52021 ttttctgcta gaaccgtatt tgcggtaata aatagctaat aaacaagcga taatgatctc
    52081 aacagccgga atcacgaggg cattacgata tgttcgagtg gaatattcat gtgtaaaact
    52141 aatcggagaa aacaaaaaat aaaaatagat acttaaaaga agatacttgt tcttcacaat
    52201 cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata ctgaacatgc cgaacaaaac
    52261 tgcataagaa agatgcagtt tatgtgcaag tactaaaaat aagctgtagg agattccttt
    52321 ggaaagtgtt gtagtcgtat actcccctag ccatttacca tccaaaatat tttgagcata
    52381 gtgaatcagc agctgatcat cataggtcgc cgacatatct gttccataga ttgctttact
    52441 atataaatac aaccgaataa ttgtaaataa tacggctatg ccaagaataa agtatgtatg
    52501 tttctttttt agttggtcga tcaaggggtt taaaatgttc agcatctttt cgtcctttca
    52561 atcacgtttt gctacttgtt tgtgaaactt ttcgtttttt acttttctat cagtataccc
    52621 tgtttgtctg aatttaaaaa gagggggcaa aaggagcatg gcgaaaacga atgcaaaaag
    52681 cactcctttg tttcttaaac aaaaaaggac aaggcagact gcccaatcct tttttgttgc
    52741 tgtcaggtag aaaacttaaa gtgtgacttc ttctgctaat aactcaggga acacttcggg
    52801 atcgaatgct tgcgctttga acatggaaat ttcttttcca tatggtgcgt aagctttctt
    52861 tttatcttta ccgacatatg gggtttctaa tattttcgaa atgttgtcaa acattggatc
    52921 atggacgatt ttgttcaatg tgtcaaatcc aattgttcca aaaccgatat tggcatgccg
    52981 atctttatga gaaccttgtg gatttttaga atcattgata tgcagtactt taagccgatc
    53041 taatccgatg accttatcaa actcttccat tacacctgaa aaatcttctt ttatattata
    53101 tccggcatca ttcgtatgac atgtatcaaa agtaacggat aatttttcat tcaattttac
    53161 tccatcgatg atttgcgcta attcctcaaa tgttcgaccg atttccgtac ctttccctgc
    53221 catcgtctcc aaagcgatct gtgccgtctg ttcttttgtc aaaacttcat tcaacccctc
    53281 aataattcta gcaatcccag cttctgcccc tgctcctaca tgagcgcctg ggtgaagggt
    53341 gatttgagta gcacctaaag cttccgcacg gacaatctct tctctcagaa attgtacagc
    53401 aaatggaaac atttctggtt tgatcgtatt tcccaaatta atgatataag gcgcatgaac
    53461 gacgatattt gttagtccgt tttctgccat aaatgctttt ccggcatcaa tattcatctc
    53521 ttcgatagct tttcttctag tattttgagg agcgcctgta tagatcataa aggtcgaaga
    53581 tccgtaactg gctgcttctt ctgccgcgcc taacagcatt ttctttccgc tcatgctgac
    53641 atgtgagcct aataacatgg atggattcct ccttgaaagg caattgtttc gaacaattcc
    53701 tgtatattga taagatgatg tacaaagaaa atggtttgtt tttctttgcc ctgttctcca
    53761 tagtaactga aactttccct aaagaaaagc ttaagaactc tttcataaaa aagatcattt
    53821 cctcacgttt tatctcattc gaaaaagaaa ttcctgtcaa aaacagcctt gtctgttttt
    53881 ttgtcctatt atttgctata attagcgaaa taaaaccatt agttgaggtg acccttttgc
    53941 cacgcaaaaa tactcgaaaa aaacagaata gaagaaaaaa agaaaaatgg tttgttccta
    54001 agattatttt tagagtgttt caatcattga ctgtttttat tactgttctt cttttgttgt
    54061 tcgcagctct aggtatcgga ataggtgctg gctactttgc ctatcttgtc gaagacacac
    54121 aattgccgac taaaaaagaa ctgcagacag aactaggaaa cattacggaa acgtcaaaga
    54181 ttgtttatgc agataataca gagatatcaa aaatccagac agatctgatg cggacgacta
    54241 tttcatctga taagatttcg cctttattga aaaccgcgat tatcagtaca gaagatgaat
    54301 acttcgataa acatcaaggt tatgtcccta aagctgtttt acgagcacta gtttcagaag
    54361 cgactggcat cggctcgtct ggaggatcca ctttgacaca gcaattagtc aaacaacaga
    54421 tcctaaccga tgaaacaaca ttcaaacgta aagcaaacga gatcgttctt tctgcacaag
    54481 tagaaaaaca cttttcaaaa aatgagatca ttactacgta tttgaatgtc tcaccatttg
    54541 gtcggaataa taaggggcaa aatattgctg gagtccagga agctgctaga ggaatctttg
    54601 gtgtagatgc aaaggatgtg actctgccac aagctgctta tatcgctggt ttgcctcaaa
    54661 gccctatcac gtatagtcca tacacgaata caggcgcgct aaaagaagat ctttctgccg
    54721 gtcttgccag aaaagatttt gttttattca gtatgtaccg tgaaggacaa attacgaaag
    54781 aacaatacga agaagcgaaa gcctatgatc ttacaaaaga ctttctccca caacaaatcg
    54841 cggaacagac tgacagagaa ttcttgtatt ataccgtgat gaacgaagca acaaggatta
    54901 ttgcacaaca attggcagaa aaagataatg ctgatatgtc agattcatcg gtcagtgaag
    54961 cttattatca aaaagcacag caaacgatcc aaaacaaagg atatacgatc cattcgacga
    55021 tcgataaaga tatctatgct gcattacaaa ccggcgtaga aaattatggc tacatgttag
    55081 atgatggtgc tggtagccaa gtggagactg gaaatgtatt gatggataac agaaccggac
    55141 gtatctatgg cttcgtgggt ggtcggaatt acagccagaa tcaaaataac cacgcctttg
    55201 atacagaacg tcaagctggt tcatccatca aaccggttct tgtttacggt cctgcaatcg
    55261 atatgggact tgtcggatca gaatcccgtg tatcagatta tgcaacgact tggcaagaag
    55321 gaaaaaatgc tggtgagaaa atcgtcaatg cgaccaatga agggtcgaac accttccaaa
    55381 ccgtccgtga gtcactggaa tggtccaaca atatcccagc ttatcactta tatcaagatg
    55441 tgttgaattc cggtggatcg aaacaatacg cttacgagca ctacttggcg aagatgaact
    55501 acccagcaaa tgataattgg ggcgttgaat cggctccttt agggacagta gatgtaacga
    55561 cgcttcaaca gacaaatgga ttccaggcct tagcaaatgg tggagtctac gaagaaggct
    55621 atatcattga ttccatcaca gataatgcag gtaatgtgat ttataaacat gaaagtaatt
    55681 ctgtccgtat ttattccgaa gcaacggcat cgatcatgaa tgacatgatg cgtagtgtga
    55741 tcaatgaaaa aatcactaca ccattcaaag atgtcatctc aagcttaaac ggaaatctag
    55801 gaaatgcaga ttgggtcgga aaaactggtt ctaccaatga ataccgagat tcatggctga
    55861 tcgtctcaac tccatctatc actatcagta gttgggcagg acacgatgac aatactggaa
    55921 tggacagcca agcaagaata cgttcggcta attatcttgc caatctaatc aatcaagctt
    55981 accaagcaaa ccctgagatt ttcggcactg atgagaaatt tgacttatca tccgatgtga
    56041 tgaagaaaaa agtatctggg ttcacaggac aaacagctgg aaaagttact gtcaataaaa
    56101 aatccatcca aacgccagga aaaacagtgg agtcactttg ggcaaaaaat ggtccgaaga
    56161 aaagcgcctt taaattcggc gtaggcggta ctgatgaaaa ctatacagat tactggaaca
    56221 ccgcttcagc ttatgctaga gcgaatccag agaccaaaga atcagacgat aaagatgatt
    56281 gatctaatag attaatcaga attaaaggga gctgcgacaa aaatcttgtc acagctccct
    56341 ttttccgaat aaatggtgtt caaaagctta catctacgat attaagacaa atttatgtaa
    56401 aaatgtgaaa aac
//
LOCUS       DAFELO010000010        56365 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFELO010000010
VERSION     DAFELO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.010"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            308..1153
                     /locus_tag="LP452_000691"
     CDS             308..1153
                     /locus_tag="LP452_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2013444.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1434..2654
                     /locus_tag="LP452_000692"
     CDS             1434..2654
                     /locus_tag="LP452_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL2013445.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2651..5371
                     /locus_tag="LP452_000693"
     CDS             2651..5371
                     /locus_tag="LP452_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2013446.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5355..6299
                     /locus_tag="LP452_000694"
     CDS             5355..6299
                     /locus_tag="LP452_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2013447.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6368..7378)
                     /locus_tag="LP452_000695"
     CDS             complement(6368..7378)
                     /locus_tag="LP452_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2013448.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7558..7902)
                     /locus_tag="LP452_000696"
     CDS             complement(7558..7902)
                     /locus_tag="LP452_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2013449.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7895..8329)
                     /locus_tag="LP452_000697"
     CDS             complement(7895..8329)
                     /locus_tag="LP452_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL2013450.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8467..9204
                     /locus_tag="LP452_000698"
     CDS             8467..9204
                     /locus_tag="LP452_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013451.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9197..10408
                     /locus_tag="LP452_000699"
     CDS             9197..10408
                     /locus_tag="LP452_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013452.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10612..11394
                     /locus_tag="LP452_000700"
     CDS             10612..11394
                     /locus_tag="LP452_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL2013453.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11464..12126
                     /gene="trmB"
                     /locus_tag="LP452_000701"
     CDS             11464..12126
                     /gene="trmB"
                     /locus_tag="LP452_000701"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL2013454.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12224..13978)
                     /gene="ade"
                     /locus_tag="LP452_000702"
     CDS             complement(12224..13978)
                     /gene="ade"
                     /locus_tag="LP452_000702"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL2013455.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKEHLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14110..15039
                     /gene="tadA"
                     /locus_tag="LP452_000703"
     CDS             14110..15039
                     /gene="tadA"
                     /locus_tag="LP452_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL2013456.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14996..16045
                     /locus_tag="LP452_000704"
     CDS             14996..16045
                     /locus_tag="LP452_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL2013457.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16042..16320
                     /locus_tag="LP452_000705"
     CDS             16042..16320
                     /locus_tag="LP452_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL2013458.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16295..16765
                     /locus_tag="LP452_000706"
     CDS             16295..16765
                     /locus_tag="LP452_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL2013459.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16782..17084
                     /locus_tag="LP452_000707"
     CDS             16782..17084
                     /locus_tag="LP452_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013460.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17101..17472
                     /locus_tag="LP452_000708"
     CDS             17101..17472
                     /locus_tag="LP452_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL2013461.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17444..17803
                     /locus_tag="LP452_000709"
     CDS             17444..17803
                     /locus_tag="LP452_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013462.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17975..18982
                     /locus_tag="LP452_000710"
     CDS             17975..18982
                     /locus_tag="LP452_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2013463.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19007..20191
                     /locus_tag="LP452_000711"
     CDS             19007..20191
                     /locus_tag="LP452_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL2013464.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20628..22211)
                     /locus_tag="LP452_000712"
     CDS             complement(20628..22211)
                     /locus_tag="LP452_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2013465.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22789..22932)
                     /locus_tag="LP452_000713"
     CDS             complement(22789..22932)
                     /locus_tag="LP452_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL2013466.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23155..23634)
                     /locus_tag="LP452_000714"
     CDS             complement(23155..23634)
                     /locus_tag="LP452_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2013467.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23707..23991)
                     /locus_tag="LP452_000715"
     CDS             complement(23707..23991)
                     /locus_tag="LP452_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013468.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24069..24263)
                     /gene="ssrS"
                     /locus_tag="LP452_000716"
     ncRNA           complement(24069..24263)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LP452_000716"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24335..25621)
                     /locus_tag="LP452_000717"
     CDS             complement(24335..25621)
                     /locus_tag="LP452_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL2013469.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25759..26430
                     /locus_tag="LP452_000718"
     CDS             25759..26430
                     /locus_tag="LP452_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL2013470.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26396..26884
                     /locus_tag="LP452_000719"
     CDS             26396..26884
                     /locus_tag="LP452_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL2013471.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26897..27844
                     /gene="prmA"
                     /locus_tag="LP452_000720"
     CDS             26897..27844
                     /gene="prmA"
                     /locus_tag="LP452_000720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL2013472.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27845..28609
                     /locus_tag="LP452_000721"
     CDS             27845..28609
                     /locus_tag="LP452_000721"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL2013473.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28842..31055
                     /locus_tag="LP452_000722"
     CDS             28842..31055
                     /locus_tag="LP452_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL2013474.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31066..31512
                     /locus_tag="LP452_000723"
     CDS             31066..31512
                     /locus_tag="LP452_000723"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL2013475.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31683..32942
                     /locus_tag="LP452_000724"
     CDS             31683..32942
                     /locus_tag="LP452_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL2013476.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33031..33978)
                     /locus_tag="LP452_000725"
     CDS             complement(33031..33978)
                     /locus_tag="LP452_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2013477.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34372..35664
                     /locus_tag="LP452_000726"
     CDS             34372..35664
                     /locus_tag="LP452_000726"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL2013478.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35679..37448
                     /gene="aspS"
                     /locus_tag="LP452_000727"
     CDS             35679..37448
                     /gene="aspS"
                     /locus_tag="LP452_000727"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL2013479.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37557..38432
                     /locus_tag="LP452_000728"
     CDS             37557..38432
                     /locus_tag="LP452_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2013480.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38698..40581
                     /locus_tag="LP452_000729"
     CDS             38698..40581
                     /locus_tag="LP452_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL2013481.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40656..40940)
                     /locus_tag="LP452_000730"
     CDS             complement(40656..40940)
                     /locus_tag="LP452_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL2013482.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40930..41664)
                     /locus_tag="LP452_000731"
     CDS             complement(40930..41664)
                     /locus_tag="LP452_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL2013483.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41793..42416
                     /locus_tag="LP452_000732"
     CDS             41793..42416
                     /locus_tag="LP452_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL2013484.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42608..43729
                     /locus_tag="LP452_000733"
     CDS             42608..43729
                     /locus_tag="LP452_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL2013485.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43736..46864
                     /locus_tag="LP452_000734"
     CDS             43736..46864
                     /locus_tag="LP452_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL2013486.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46883..50077
                     /locus_tag="LP452_000735"
     CDS             46883..50077
                     /locus_tag="LP452_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL2013487.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50223..50930)
                     /locus_tag="LP452_000736"
     CDS             complement(50223..50930)
                     /locus_tag="LP452_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2013488.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51695..52093
                     /gene="spxA"
                     /locus_tag="LP452_000737"
     CDS             51695..52093
                     /gene="spxA"
                     /locus_tag="LP452_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL2013489.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52425..53078
                     /locus_tag="LP452_000738"
     CDS             52425..53078
                     /locus_tag="LP452_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL2013490.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53304..54302
                     /locus_tag="LP452_000739"
     CDS             53304..54302
                     /locus_tag="LP452_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL2013491.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54478..56286
                     /gene="pepF"
                     /locus_tag="LP452_000740"
     CDS             54478..56286
                     /gene="pepF"
                     /locus_tag="LP452_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL2013492.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18541 a         9726 c        11681 g        16417 t
ORIGIN      
        1 atagttcatc tttttttgtt taaatctttt gctaagttga actaatagag tcttttatga
       61 atatcgaagt tagtttttca cctaacttcg ttttttatag ttggttatta taaaatagta
      121 atatagctga tttggattca attcatattt atttataaat caaaaataca gagaatagta
      181 tataaatgtc ctttcctatt cttaaaatga tgtttgtact tacttaaatt aggtataatt
      241 agtatatgtt ttgtgagaaa tttagactta caataagcag tgaatttata atcaacgtat
      301 aggagacatg gatgaaatga aactaattgg tatagattta gacggtactc ttttgaattc
      361 tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa catttaccgg aagactgttt
      421 tccattcatt tgttctggaa gagaagtaga agacattaat aatattctca aaaaatctgg
      481 tttaaggata ccagcagtag ggttaaatgg ttctgttgga tttgatggag aaaaaaagat
      541 atttgagtct tccttcgata aaaatgatgt aaaaaaaatt gattctgtcc tatcgaaatt
      601 tccaaccaaa atatatacaa atctcggaag ttatgaatct cagaattata aaaataaaat
      661 gaaaaaaatt tttcacgagg tagggagtga attttctatc aaagagctaa actatgagct
      721 tgagtatgaa aaatctattt gttcaatccc ttataataat attgaggaaa ttatagaaaa
      781 ggatgctatc agcatttaca aattttttat ttttattcct aatcgtcaat tgaaagctaa
      841 tatatttaac catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg cacctgtaaa
      901 tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt tggaatcaat
      961 ctacaattta aagaaacctt tgagaatcgc gatcggagat agtttaaatg acttatctat
     1021 gtttgaaagt gcagatatta gttttgcaat gggaaatagt catcaagaaa tcatgaaaaa
     1081 agctgattat gtcactgttt caaatgataa agacggagta tctcaagcat ttgaaagaat
     1141 tcttaccata taaactaatt aaggtagtat agaacataga attctaaata cggaaaaatg
     1201 gagcataaat tccagtacaa atagtacttc ttcagtgtca aacgaagcga atataacata
     1261 gatatttagg attgtgacaa aaatcatgtc acaatctttt tttcgaataa acttattttt
     1321 tgaagtcggc catttatcgt aatttctttg cataatacga acaaattttc ggttattgat
     1381 gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag gaggaaattt ttaatggtga
     1441 aatttatcca tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg
     1501 ataaaactgt acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg
     1561 aacaggcaat aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa
     1621 atcgtccttc attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga
     1681 accatatacc tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt
     1741 ggttctcctt tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa
     1801 taacgataaa aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga
     1861 ttcaaaaaga caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt
     1921 tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa
     1981 agatgcaaga aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct
     2041 taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac
     2101 aagcttctac atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga
     2161 ttaaagtagc agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga
     2221 caaaagaggt cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt
     2281 taattgagat aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac
     2341 aatcaggaga gctattagac tatttgttag aggaattctc agataggata aatgatttct
     2401 ttatttggcg tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt
     2461 ctgctaaatt gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa
     2521 ttttaaatga agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat
     2581 atcaagaaga aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg
     2641 aggatcagga atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa
     2701 aagttttgac ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc
     2761 aacgatttat caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa
     2821 aaaggattat acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc
     2881 tgtatatggt gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt
     2941 gtggctgggg gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac
     3001 gaaagagtta ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca
     3061 tttgagagaa aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca
     3121 agtattccaa aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg
     3181 ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca
     3241 tcaaaaacaa gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga
     3301 gtcgcaacag cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc
     3361 cagacagcaa gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag
     3421 cactacaggt cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta
     3481 tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat
     3541 ggatcagcag tcagccagat attattttta tttagagcag gagcaggaac taaaagatct
     3601 ccagcaactt caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga
     3661 gactagagaa acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa
     3721 tccgttaccg gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag
     3781 aaggattgct gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca
     3841 agagatgatt gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa
     3901 tcagtcagag atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc
     3961 aataatcatt gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg
     4021 tgcaggcatg attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga
     4081 tttctctgag ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc
     4141 tcaattagct gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca
     4201 gcaagaagta tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact
     4261 catgaaagaa tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg
     4321 gaaacataaa agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaatttga
     4381 gtttgttaaa gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct
     4441 ttcccgtttt atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac
     4501 tttattgaag caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata
     4561 cagcgaacaa ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca
     4621 aaaagccaaa caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac
     4681 tccactattt cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa
     4741 agaaaagcaa tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact
     4801 ccaacttcaa gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc
     4861 tagccggcaa aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc
     4921 cgggacattc ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt
     4981 aagagcagca ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact
     5041 gaaaaatgat acactgactt taacagacgg atatacagaa tttcccatct atgagttatc
     5101 tacaggtacg aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc
     5161 taaccgttcg atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg
     5221 aaagtatcaa ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt
     5281 ttcttctgat aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt
     5341 agaaggagca aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat
     5401 ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt
     5461 ttactttcca agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg
     5521 aaatcaataa atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc
     5581 aaggaaaccc tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag
     5641 agcctagttt atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca
     5701 atcaattgtt gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta
     5761 ataaatatca aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg
     5821 ccagtggatt agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg
     5881 aatatcctga gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa
     5941 aggtgattga actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg
     6001 gtcatctggt attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg
     6061 aagctgacga agatgttctc gtattacgtc atatggtttt agcacaccat ggcttattag
     6121 aatatggctc acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca
     6181 atatcgatgc ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat
     6241 acaccgatcg gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc
     6301 aaaaatttat ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg
     6361 ttttctttta ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag
     6421 ttgattttgt gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg
     6481 atgaagttgt tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat
     6541 ttgcatcttt taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag
     6601 tttcagtagc gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt
     6661 ggtttttcac cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag
     6721 taataacatc ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag
     6781 gaatagttgt tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat
     6841 cagttgattt gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca
     6901 catcttttgc ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga
     6961 aagatttcca agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag
     7021 cttcaaaagc taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag
     7081 cttctaattg tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat
     7141 ccacttgctt atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca
     7201 tccgttgtac taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata
     7261 cagtgatcgt tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg
     7321 ctagagttaa aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta
     7381 aaatttgcac tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt
     7441 cctttaaaaa cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag
     7501 gaaatacgca aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta
     7561 tatttgttca ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg
     7621 tgtgatacgt ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg
     7681 gaaagcagga atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg
     7741 attcaggctg ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag
     7801 tttctttctt gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc
     7861 agcagctcca aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta
     7921 cgattgtttc ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa
     7981 agtgaataga aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa
     8041 aaacagattg ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg
     8101 gaaaagcttt ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa
     8161 gttcgggatc atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc
     8221 ctttcgttgc ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat
     8281 agcttgggat ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc
     8341 tttctataaa gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga
     8401 aaaaaacaaa ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga
     8461 aaaagaatga gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta
     8521 aaagatatca gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga
     8581 gcagggaaaa gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg
     8641 atagaaatca atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg
     8701 tatattccag agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc
     8761 actgcgatgg cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg
     8821 gagacttttc ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg
     8881 aaacagaaag tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac
     8941 gaaccatttt taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa
     9001 atgaaaaaag aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa
     9061 tattgtgatc gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag
     9121 atgttgagaa aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta
     9181 acaaaggagc aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa
     9241 aatgatgaaa tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt
     9301 gattggcggt gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc
     9361 aattggaggg ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc
     9421 tacttttgca gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac
     9481 ctatttggaa cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc
     9541 atgcggattg gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt
     9601 cgtctatctg atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga
     9661 attgtttcga ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc
     9721 tctgatcttt ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt
     9781 agtacaggca agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg
     9841 ggagaaaatg gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt
     9901 attcacagat gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat
     9961 attgactcat attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt
    10021 ggcaagaggt tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt
    10081 gattgccgga gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt
    10141 gatcggtttc caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct
    10201 ttatccaatc cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt
    10261 aattatcgcg tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg
    10321 atgggttccg ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc
    10381 aactcgtttg aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg
    10441 aaaaatagag cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt
    10501 tttttgaatg gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc
    10561 gactagaata ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag
    10621 tttcagttag accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac
    10681 ataggcatgc gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct
    10741 gctctttcaa gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc
    10801 gatactttga cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt
    10861 caaagaaacg acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc
    10921 atgctgaccc gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa
    10981 aaagaattac cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt
    11041 cgtgagcaga tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac
    11101 catcgtaatt ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg
    11161 tttgcagacc ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc
    11221 tggaatcagt ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt
    11281 cattggtatg cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat
    11341 catcgacgga tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg
    11401 gcccaaactc tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa
    11461 ataatgcgtg tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt
    11521 gtaattagtg atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat
    11581 ccaatccaca ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc
    11641 catcctgaaa tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta
    11701 gacaagttga tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg
    11761 ttgacagagt atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca
    11821 tggccaaaaa aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat
    11881 gaacagattc ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt
    11941 gagtattctc tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat
    12001 ttacatcaaa gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca
    12061 aaaggtcaac gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt
    12121 ctgtgaaact aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa
    12181 atgaacatta cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt
    12241 catcggtttt tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt
    12301 cggtgatctt caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat
    12361 caaaggtttt agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat
    12421 agacgatcga aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta
    12481 tttcctgctg tttgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag
    12541 catacagcat atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa
    12601 tcgttgtcgc tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt
    12661 ttcccgtttg atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag
    12721 gaatgaatcg ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat
    12781 tcgggataat ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg
    12841 gatttaatgg ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag
    12901 caaataaatg gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt
    12961 catgaattgg cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg
    13021 caatcgcacc tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa
    13081 tggcttgtaa tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc
    13141 cttcttttac taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat
    13201 tttcaggtgt gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa
    13261 ccattaaata cataccaaga tccaaatgtt ctttagcttc ttttgcagtt gtagcttcat
    13321 gatccgtccg aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg
    13381 catgcccgtc aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg
    13441 tttcattatt tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt
    13501 tttgtaaaaa aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg
    13561 ttacaggtac acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt
    13621 ctatcataaa ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca
    13681 ctacagtagt aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg
    13741 agctttcgat atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat
    13801 tatgtacgct ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta
    13861 catctttttc catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta
    13921 ggtcagcagg ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg
    13981 gagtgcctcc caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt
    14041 ttttgtaaat tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag
    14101 gggagaataa tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa
    14161 catgcccaag acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat
    14221 ggaaaaaaac gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca
    14281 agatttaagt atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca
    14341 tttacttatt ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac
    14401 cttcaacgag agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt
    14461 tgctttagtt ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac
    14521 cttttcagcg gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa
    14581 gaaaaattgc aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg
    14641 cagctacaga tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta
    14701 cggcatcgtc cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct
    14761 gcggtaagag cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt
    14821 gaaggtacat tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt
    14881 ttagctggtg tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta
    14941 tggagttatc aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa
    15001 gaagctcagg aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca
    15061 aaaccagttt gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc
    15121 cctttattta ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca
    15181 tcagctaaag gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga
    15241 gcaactggta cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga
    15301 aattgccaaa cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc
    15361 atatccgatg ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt
    15421 tttgcctcag cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat
    15481 acaagtcttt cattggtatg ttctactatt catcctagcg gttactctat tggctttact
    15541 agtaaggtat cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca
    15601 attattgatt ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg
    15661 aaagcttttt catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa
    15721 acaagaaagt ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat
    15781 gtcattggct gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt
    15841 tttacaggga gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac
    15901 aagaaaacag cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt
    15961 tttgggcgta gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg
    16021 aagcatagag gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca
    16081 ttgatcgaaa tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct
    16141 aatttgtcgc gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg
    16201 gtagacacac aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag
    16261 gaattgttga atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga
    16321 aggtaaggtt ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact
    16381 tacaacgatc ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt
    16441 ctatcaattt ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt
    16501 gaatcagatc caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt
    16561 tccgaatcca gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa
    16621 acgccatgct tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta
    16681 tcaattttat tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg
    16741 tcgctataca aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc
    16801 ttatgatcgc ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg
    16861 cagaaaagaa aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga
    16921 cgcagcggat gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc
    16981 aaattgtcga taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga
    17041 tcagtattga aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg
    17101 atgatattga gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta
    17161 agtgaatttt tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa
    17221 gaagaactca agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc
    17281 gaggataaac agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc
    17341 ggcggacatc aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac
    17401 acaatttctt tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa
    17461 aacgatcagt agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct
    17521 tttatgtacg tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa
    17581 tatacgaatc aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca
    17641 gtctacgctt gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata
    17701 tcagtgggta caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt
    17761 cacagaagaa gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa
    17821 cgccctcttt tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata
    17881 aaaaacagtg aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa
    17941 aattgttata atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg
    18001 gtttaatcaa aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt
    18061 agaagcctat gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga
    18121 tggagtgccg acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa
    18181 actttctttc gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag
    18241 ccagacagaa catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt
    18301 tgtttttctt attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat
    18361 tgcggcagga atgggcaact tgttattaac agttctttta aacctatcga atgcggggta
    18421 ccaaacagaa ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac
    18481 aagtgatttg acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt
    18541 aatagatcct gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga
    18601 aaaagcaaaa gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt
    18661 gctagagcaa tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag
    18721 tggattttta gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta
    18781 tcttcaagga atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag
    18841 tattttgatc ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt
    18901 gaaacttgct tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa
    18961 ctggaaatca tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat
    19021 tgcaatcaat gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac
    19081 cgtagttgcg aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa
    19141 gtacggcgat aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt
    19201 aaaaatgctg ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac
    19261 cggagtaggg caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga
    19321 tgagaaagta atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc
    19381 aaacttaatg gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt
    19441 attcgataca tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt
    19501 gtcttattat gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta
    19561 tgtgtcggaa cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac
    19621 ttgccactta ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac
    19681 ttctatggga ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga
    19741 tccagctgtt ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa
    19801 tgttttgaac aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga
    19861 tcttgaaaag aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg
    19921 catccgaaaa tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt
    19981 tactgctggt atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc
    20041 ttggtttggt tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat
    20101 ctctacaaaa gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat
    20161 tgcccgtgat gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga
    20221 aacgcatgca agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt
    20281 aatttaactt caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa
    20341 agtggtcaaa agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat
    20401 tttattttca ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag
    20461 atcagcctcg tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc
    20521 gaaacattca ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg
    20581 tgaaagcaca ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt
    20641 tggccgagcg gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt
    20701 tgctgcaaaa agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc
    20761 atttcttcaa tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc
    20821 aggacgttga ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg
    20881 gataaatctt tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc
    20941 cgtcgagcga cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg
    21001 ggatcggcaa actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg
    21061 atggtgccac cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct
    21121 gcaccattgt cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct
    21181 ttgaatagta cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg
    21241 ccagattttc caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg
    21301 acgagtgtca gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca
    21361 ttgtctcctt catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact
    21421 tctttttgct gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact
    21481 cgaaaacggc tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt
    21541 tttgtggtga cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt
    21601 gtattctgta aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt
    21661 tcatcgagta aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact
    21721 cgttcgcctc cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct
    21781 tgcaagatcg ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc
    21841 tgcagctcac cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat
    21901 gcttcgtagc gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag
    21961 tagtggttga cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag
    22021 ccttttttgc gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa
    22081 aaggttgatt tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt
    22141 tgacaggata aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct
    22201 tgtatgatca tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct
    22261 gtgttattta aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg
    22321 tgaattcaac tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat
    22381 ggctaaatgc gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca
    22441 tcatagtatt ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa
    22501 agttcaacta gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct
    22561 ttttttatct gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc
    22621 agcgttcagg cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt
    22681 gccatttgct taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc
    22741 gcgttcagga gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa
    22801 tgatttaccg aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag
    22861 tttggcgata aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc
    22921 ggaaacttcc acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca
    22981 ccagggcgac taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg
    23041 ttttcccttt attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt
    23101 atcacggttt tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc
    23161 tttggtgtgg tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc
    23221 gcaagctgcg tttctaataa catattcaga aacagaacca ataaataaac gttctacagc
    23281 attcaatcct gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct
    23341 ggcaataatt tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc
    23401 tttcttcgca ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc
    23461 tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc
    23521 taatagaagt tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc
    23581 agcttcatca gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat
    23641 tcactcctca agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag
    23701 aattatttac tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga
    23761 aacaataatt acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt
    23821 taaaataccc attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg
    23881 atttttatca tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata
    23941 gctaacgaat aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc
    24001 ctccaaaata agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt
    24061 aaaagatggt aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc
    24121 aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc
    24181 agtgacacaa gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga
    24241 caattcgaac atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat
    24301 gagagagctc gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc
    24361 actcttttat acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg
    24421 gctgataata ctgagcactc ttcagtttat ctggtaaata ttgctgattg acccaagcct
    24481 gatcaaaatg atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt
    24541 aatggctatc tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa
    24601 gtgctgcatc taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga
    24661 cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag
    24721 cgttcactgt acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa
    24781 ccatcagacg ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat
    24841 gaagtgcagc atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat
    24901 aatgagcatc tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga
    24961 tcgacagtgt aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt
    25021 ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag
    25081 catcttcgtc taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat
    25141 gttcgactgc caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac
    25201 ggctgcggat cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga
    25261 tgattcggcc actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt
    25321 gtacttcatc caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt
    25381 gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc
    25441 cggcgatcgc acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg
    25501 aagagagcat gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct
    25561 gctgaccaac gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca
    25621 taggattttc ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc
    25681 tagaaagaac agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg
    25741 ataaagtagg tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa
    25801 acgacgccag aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa
    25861 ggaagattag ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc
    25921 gcccatagct atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt
    25981 acgatctacc tttacacgat gcatactcat cggattttga acatcattac tcagccagaa
    26041 gggattcctc aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg
    26101 agtaaaaatc gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg
    26161 gaagcattag caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc
    26221 ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc
    26281 ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat
    26341 atctctttac aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa
    26401 aaagaaggat ctattaacat atttagatga aataattgaa aaacaagtga cagactatga
    26461 tgtagcttta gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc
    26521 agaaaataaa gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat
    26581 tatcgaattt gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg
    26641 caattatcta gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga
    26701 ttctttagtg gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt
    26761 tttggatgaa gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc
    26821 agaattagta gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta
    26881 ttaaggagtg acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt
    26941 cgaagcagtt gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc
    27001 tttagatgtt gaacattttc agtcggatca atatggtgag attttagata aaaatgattt
    27061 tacatcgctg aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc
    27121 ggaaatcttg ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat
    27181 tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa
    27241 gaaatactat catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa
    27301 ctaccagaca gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg
    27361 cacaggcact catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg
    27421 tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata
    27481 tcttggtgca aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa
    27541 agaaaatatg gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt
    27601 gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt
    27661 attgatgatc ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg
    27721 aatcattgaa gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt
    27781 ggatcaaatt ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga
    27841 atagatgcag cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga
    27901 aggggaaaac tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt
    27961 tttggcattc aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc
    28021 tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac
    28081 catagcgagt ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga
    28141 attaggagcg caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc
    28201 aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca
    28261 atcccatcgg cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga
    28321 gcggctagat gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga
    28381 aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt
    28441 ctttggacca gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg
    28501 gatactttgt ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt
    28561 aagtgctgct agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt
    28621 aataaaagag tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac
    28681 aattatttat acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt
    28741 ccgtaaaaaa acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc
    28801 gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt
    28861 taacgggacc cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat
    28921 ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag
    28981 gcgaacccta cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg
    29041 atccccatac agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt
    29101 tagaagatct gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca
    29161 aattagggaa aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga
    29221 tgctgctagc aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac
    29281 ataacatgcg tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa
    29341 ctttagaaat ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat
    29401 tagaagatac agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga
    29461 tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa
    29521 tagcgacaga agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt
    29581 caatctatcg taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag
    29641 ctattcgagt gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca
    29701 caaaatggaa accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata
    29761 tgtaccaatc tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa
    29821 tccgaacaca ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata
    29881 aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc
    29941 atgagatact cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa
    30001 aaggagatat ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt
    30061 taccaaaagg atccggacca ttggactttg catacagtat ccatacagat atcggtaaca
    30121 aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg
    30181 gagatatcat tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga
    30241 aattagttgc tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc
    30301 gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat
    30361 ttacgcctaa agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt
    30421 atcaaacaga agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga
    30481 tggccaatcg tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag
    30541 aagcagaaga aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg
    30601 aaggtggcgt tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta
    30661 atccgattcc tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc
    30721 atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg
    30781 aagtcgaatg ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt
    30841 acggctataa tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga
    30901 aaaatctcaa cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga
    30961 cggttggtat ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac
    31021 ctgatgtcta cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt
    31081 caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac
    31141 ggcctacttg ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg
    31201 gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt
    31261 gtagaagatg tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg
    31321 aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta
    31381 tacgaatcat tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc
    31441 ggagcagata tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct
    31501 aaaaacaaat aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt
    31561 tagactgata aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca
    31621 gcagtcggaa aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga
    31681 agatgaaaga agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa
    31741 aaggatacaa catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa
    31801 agattttcaa taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg
    31861 atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg
    31921 gtcaaggtgg agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta
    31981 tggatctaat gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga
    32041 cacaagcact tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt
    32101 tctttacatc tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg
    32161 tcgaaaaatt agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg
    32221 atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg
    32281 aagaagctcg acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca
    32341 atgccacaga tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga
    32401 acatcatccg tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt
    32461 tcagtagacc agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt
    32521 ttattcaaga aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga
    32581 aagaagtaga agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat
    32641 atggaacaga ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt
    32701 atgtagggaa agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg
    32761 ggggaaattt tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac
    32821 gtgtgttttc cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga
    32881 cgattccatt caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt
    32941 agattagcat aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc
    33001 atattactat aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt
    33061 ttgataatct tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc
    33121 tggacgctta gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa
    33181 aacaagttgc gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc
    33241 ggttaaaatt tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc
    33301 ttcaccaaat tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag
    33361 aggaaaatag ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc
    33421 agcctgccga tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt
    33481 cgcttctttc aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc
    33541 ttcttttaat tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa
    33601 gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga
    33661 ttgtttgaga aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc
    33721 ttttgtagcg atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat
    33781 cagttcttcg gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct
    33841 attcaaagct gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt
    33901 atgtcctcca acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc
    33961 gattcttatt tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa
    34021 aaagctgaac taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac
    34081 tggtttttag taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga
    34141 aactttacaa gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga
    34201 cactctggag atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa
    34261 agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga
    34321 tgaacaaggg tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc
    34381 caaagaccaa aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc
    34441 gaagaaacag cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt
    34501 tttgaacact atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa
    34561 gagatgtatg atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca
    34621 gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg
    34681 ttcaaaactt tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta
    34741 cgtcaattcc atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg
    34801 gagagcatgg tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta
    34861 gtcatcaatt cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac
    34921 tatcttttac cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat
    34981 ccattacgcg tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca
    35041 tctatcttag attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg
    35101 ctggatgcat tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat
    35161 tacacacata cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg
    35221 atttgcgcag gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct
    35281 ggcttcggat ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta
    35341 gttatccctg catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat
    35401 atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac
    35461 ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta
    35521 gtattggtga tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca
    35581 agccgaaaag aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat
    35641 gatgaaatga ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt
    35701 gcggattagt ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa
    35761 aaagacgaga ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc
    35821 aaatcgtctt taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca
    35881 gtgaatacgt gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc
    35941 ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag
    36001 caaaaacccc gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga
    36061 aatatcgtta tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg
    36121 cgacaacaaa agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac
    36181 cttacctagg gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc
    36241 atgctggaca tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga
    36301 atgctggatt cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg
    36361 gagaccgtca gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag
    36421 aaatccaaac ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg
    36481 aagtcacatt gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg
    36541 acaaaccgga tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag
    36601 tagaattcaa agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg
    36661 ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag
    36721 gacaattcgg agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc
    36781 caattgctaa attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac
    36841 caggtgactt gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg
    36901 ctatccggac acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct
    36961 tatgggtaac tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg
    37021 ctcaccatcc attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg
    37081 caaaagtcta tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt
    37141 ctatccgtat tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa
    37201 aagaacaaat ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc
    37261 cacatggcgg tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata
    37321 atattcgtga agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg
    37381 caccaagtat ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag
    37441 aagaatagcc gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg
    37501 atttaattga aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga
    37561 aaaaaacatt cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg
    37621 tttatgcgtt gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt
    37681 caagtggtat tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat
    37741 tcgaagtacc ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt
    37801 ggttaaaaat cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc
    37861 tttgtatcca tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt
    37921 ttgggggggc tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg
    37981 gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta
    38041 tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat
    38101 atatgtttta cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata
    38161 ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg
    38221 aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata
    38281 agcatgataa gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta
    38341 gagaagcaat gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga
    38401 tacttggtcg tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata
    38461 ctgctgtaga cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa
    38521 gaaaaactgc ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt
    38581 taaatttatg ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt
    38641 tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg
    38701 gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca
    38761 ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat
    38821 ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag
    38881 gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga
    38941 ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag
    39001 cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac
    39061 tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt
    39121 acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt
    39181 gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca
    39241 tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt
    39301 gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt
    39361 gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat
    39421 atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat
    39481 tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt
    39541 gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact
    39601 tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat
    39661 gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca
    39721 ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt
    39781 gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag
    39841 agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt
    39901 acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa
    39961 agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac
    40021 caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta
    40081 gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt
    40141 tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac
    40201 attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg
    40261 aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa
    40321 gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg
    40381 cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt
    40441 aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca
    40501 cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt
    40561 gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc
    40621 ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg
    40681 gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct
    40741 tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt
    40801 cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt
    40861 ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa
    40921 aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat
    40981 attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc
    41041 ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt
    41101 aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg
    41161 gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc
    41221 taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt
    41281 tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg
    41341 aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg
    41401 gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc
    41461 gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag
    41521 gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa
    41581 agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc
    41641 gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta
    41701 ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat
    41761 actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag
    41821 ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac
    41881 cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc
    41941 tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa
    42001 atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc
    42061 caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga
    42121 tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt
    42181 ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg
    42241 atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata
    42301 tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg
    42361 acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg
    42421 ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca
    42481 atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag
    42541 aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg
    42601 tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta
    42661 tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga
    42721 aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga
    42781 tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat
    42841 ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt
    42901 cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt
    42961 taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt
    43021 tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca
    43081 ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag
    43141 tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga
    43201 actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag
    43261 acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca
    43321 aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat
    43381 cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc
    43441 cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat
    43501 catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg
    43561 ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc
    43621 tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca
    43681 agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa
    43741 accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc
    43801 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
    43861 gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg
    43921 aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt
    43981 tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg
    44041 aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa
    44101 aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact
    44161 gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg
    44221 taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac
    44281 tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa
    44341 attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga
    44401 agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat
    44461 actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa
    44521 gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac
    44581 gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa
    44641 acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca
    44701 agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact
    44761 agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg
    44821 acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat
    44881 tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa
    44941 agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg
    45001 aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg
    45061 acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac
    45121 tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa
    45181 tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa
    45241 gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg
    45301 atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt
    45361 aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat
    45421 tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa
    45481 aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa
    45541 aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca
    45601 atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag
    45661 acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa
    45721 aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga
    45781 tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga
    45841 ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac
    45901 gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga
    45961 aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga
    46021 agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac
    46081 ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca
    46141 gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg
    46201 tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca
    46261 aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct
    46321 tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt
    46381 ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct
    46441 ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg
    46501 agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc
    46561 gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc
    46621 cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg
    46681 atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga
    46741 aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt
    46801 acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg
    46861 ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg
    46921 acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt
    46981 gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa
    47041 agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt
    47101 tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc
    47161 aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg
    47221 tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga
    47281 aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat
    47341 cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca
    47401 agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa
    47461 acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac
    47521 gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct
    47581 gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt
    47641 gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc
    47701 tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt
    47761 tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac
    47821 gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc
    47881 agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct
    47941 gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc
    48001 cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt
    48061 cgtctatggc gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc
    48121 ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta
    48181 tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata
    48241 tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag
    48301 aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga
    48361 gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga
    48421 tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt
    48481 tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga
    48541 atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca
    48601 aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga
    48661 tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag
    48721 agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg
    48781 tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc
    48841 ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta
    48901 caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg
    48961 gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta
    49021 tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct
    49081 tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt
    49141 agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt
    49201 gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga
    49261 acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca
    49321 aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga
    49381 agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat
    49441 ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg
    49501 acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct
    49561 cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt
    49621 ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc
    49681 gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt
    49741 taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa
    49801 tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga
    49861 acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat
    49921 gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact
    49981 gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat
    50041 ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta
    50101 atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga
    50161 ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa
    50221 ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata
    50281 tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag
    50341 ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct
    50401 tctggaagca tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg
    50461 aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt
    50521 tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca
    50581 ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca
    50641 agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag
    50701 tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct
    50761 cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga
    50821 tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc
    50881 tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac
    50941 gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt
    51001 ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa
    51061 ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga
    51121 acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt
    51181 ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat aaaataacgt
    51241 tatttgattt atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg
    51301 cttatataac agagaaaata agcgttataa taaagaacag tattatttaa aatgacagaa
    51361 aacattacta ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc
    51421 ttttgagata aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa
    51481 ttagatttga aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt
    51541 atattaacct tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt
    51601 aaaagttgat tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa
    51661 aagtgattct tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag
    51721 ttgtacttcc tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga
    51781 acgtaatatt ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac
    51841 agaagacgga acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat
    51901 ggatttagat gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt
    51961 gcttcgccgt ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga
    52021 aattcgtcgg ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat
    52081 ggctggatta tagaaggata atacgaaact tggaaaagac tattacaaag atagtttttc
    52141 caagtttttt tgtttttaca aaaagtattc ttgattagat gtataaaaag gtagtagaag
    52201 atgatgattg cacgaaaaat agagagggaa aattagctga aaagtcggct atatagtatt
    52261 cgtctgccaa aaatgttata gttaaataag atagcttgaa aggttgaaaa atccgctggt
    52321 tttattaaat tcgcttaaca gcgagatttt ttttctttac atttaagctg atttcgctac
    52381 aatgaatcta tattaaacat tgtggcaaag tgaggtgtag caaaatggaa atggaacata
    52441 ttaatgaaaa tacgatccgt gtactaattg gtcatgaaga tttagaggaa cggggcgttt
    52501 catttttgga tttacttggc aatcacaaag aaatcgaaaa tttcttttat agtattttgg
    52561 aagaagttga tatcgaagag gagttccgag gaagcgaagc tgtcactttc caagttttgc
    52621 caaaaggaga cggattagaa ctgtttatca gtaaaaatct tccggatgaa gaaatttctc
    52681 aatcagaaga ttctactgtt gattcagaag atgtgagtga ctttcttcgt cagcagatta
    52741 ttggcagcga tccggaggaa gaattatctg gaagcgccct agaagaacag gaaagacaag
    52801 caacttttgc ttttgaagat tttgaacaag tgatccaact tgcaacagat gtcgaactag
    52861 agtctgcttg gactgatttg tatttattcg aagagcgcta ttacctagtc gttcacttct
    52921 ggatggaaaa tctcaaccaa gcggatgtag agaatcagat tgctcgaatt ttggagtttg
    52981 ctgaaaagag cgatcgtaca ccagaaacat tgaatgaata tggacaatgt ctgatggcgc
    53041 gtaatgctat cgagcgaacg aaattttatt ttgtataaat cagcattata gtaagagact
    53101 gtgacaaaaa ttgtgtcgca gtcttttttt cgtataaatg tgttcaaaag cttgcatctg
    53161 cggtaacaag cccaaccaaa cgaaaaatat tgggaactat ttttgtttag ctgttcttat
    53221 tacttgatgc agaatagctt tttcacgccc tcttttttcg tgtaaataaa ttcttagacg
    53281 taaataagtt cagagggaga aaaatgctgc ttgcaatgaa tgaatataat aagtgctgtt
    53341 ttgctaaaca tgcagaaaaa ggaaagaaat attattgtcg tggctgtcga aaaagagtga
    53401 ttttgaaaaa gggaaagaaa aaatgtgctc attttgccca tcaaaaatca gataactgtt
    53461 cggtctttag tgaaagagaa tcaaaagagc acttacagtt aaaggaatgc ttcatggatt
    53521 ggctgggaca gtcagtagaa ccagtatttt tggaagccta tttgccgagg ttgcggcaaa
    53581 ggccggatat tttgttggcg aatctagcaa tagaaatcca atgttctaga ctgtctcacc
    53641 agagatttat tgaaagaacc caaaattatt tgaataacag ttaccaagta tggtggatac
    53701 ttggtaattc ttttcttgga caatcgcaat tctcattgat agaaaaaagt tgctgctact
    53761 ataatagaaa aagaggagtg cattgctgga aagctgatct caagaaacaa aagctttatt
    53821 tgtatcatca tattacagag actgtatcag ggcatatttc cttttgcagt agctgctgga
    53881 ctttttctag tagggacctt aaagaaatat tcactagtaa cgaaattaaa atgaaccaaa
    53941 tgaaaaaaat agaaaaagtg agtgaagatg ggaagaaatg gctgacacgc caacttattc
    54001 acaagcaaaa gaatacagta tccatacaag aacaatgcta cttgcgtcat aaacatcttt
    54061 tgcatctatc atcgtggatc tatcaaaaaa gtcggttttt cttttattta caagaacagg
    54121 tttttcttta tcgaatgttg tacgaagaag ccttggaaaa ccagaaagtt cctgatttca
    54181 actcatggct ttgccaaata aaagaacata aaagggaatg gctttttcct atgattgatg
    54241 aagagatggt ctatcagcag ttttttaacg aatgcataca cttgtcttct ttgaaaaaat
    54301 agtaagtata aaaataggct aggaatcggt tttctatgta aaacaagatt tagttatgct
    54361 aaaattgatg gaagcagaca catgaatgag tagaaaagtt agatgctgtg acatgtggat
    54421 gttagactat gatctatttt gatagataga gctaagggaa gaaaaggagt ggatttcatg
    54481 gaagtaaagc agttgccaaa acgagaagaa ttgcctgaaa atttaacttg ggacttgacc
    54541 aagatctttt caagcgacca agagtttgat gagaaatatt tggaattatc agaagagtta
    54601 aaacaatctg aaaaacacaa aggaacactt gatcaaggcg cttctcaatt tttaaatgcg
    54661 attgaattcg tattgagggt ttatcgccaa actgaagtca tttatgtata tgcgcacctt
    54721 aaaaacgatc aagacactgg aaatacagat taccaagcgc tttatgcaag agcaagcagt
    54781 ctgttttcga aagttagtga agccgtttcc tggtttgaac cagaaatatt gcaattgtca
    54841 gatgaccaga tttggcaata tttcaaagaa gaaccaaaat tggaagtcta tcgccattat
    54901 atccagcaaa tagtagataa tcgagcccat gtcttatctg ctgagcagga atctcttctt
    54961 gctggagcag gtgaaatctt tgatgcttca agtgatacat ttgctgtttt gaataatgca
    55021 gatctagttt ttccaacgat tgaaggagaa aatggtgaaa tagtccaatt atctcatggc
    55081 gtgtatggtc agttgctaga aagcacggat cgaagggtgc gcgaagcagc atttaagggg
    55141 ttgtacagtg tttacgaaca atttagaaat acatttgctt ctactttagg cacacatata
    55201 aaaggacata attttaaagc gaaagtccgt aattacagct ctgccagaga agcgtctttg
    55261 agcaataatc atattcctga aagtgtatac gatactttgg tagacgtggt aaacaagcat
    55321 ttgcctttgt tacatcgata catggaatta cggaaacgtt tattagaagt ggaaaaactg
    55381 cacatgtatg atctttatac accggtctta ggggaagctc caattacctt tacgtacgaa
    55441 gaagcaaaag aaaaagcttt agaagcactg aaaccaatgg gtgaagaata catggccatc
    55501 gtagaaaaag cattctctga acgttggatc gatgttgtcg aaaataaagg gaaacgaagc
    55561 ggtgcttatt cttcgggaag ctatgacaca aatccatata ttttattgaa ttggcatgat
    55621 acgctggatc agctatttac gcttgtccac gaaatgggac atagtgttca tagttatttc
    55681 actcgttcga accagcctta tgtgtacggc gactactcca tctttttagc agaaattgct
    55741 tcgacaacga atgaaaatat cctaacggag tatttattgg aaacagaaaa agaccctcgt
    55801 gtacgggctt atgtactcaa ccattacttg gatgggttta aaggaacagt tttccgccag
    55861 acacaattcg ctgaattcga gcatttcatg catacggaag atgaaaaagg tgtgccattg
    55921 actagtgaat acctaagtga tagttatggt aaattgaatg caaaatatta tggtccagca
    55981 gtcgaagaag acccagaaat taaattcgaa tggtcacgga taccgcattt ttattataat
    56041 tactatgttt tccaatactc gactggcttt tctgctgctt cagcactggc gaagaaaata
    56101 ctaaaccagg aaccagaggc actagagaac tacttggctt acctaaaagc aggtaacagc
    56161 gattatcctg tggaagtaat gaaaaaagca ggagttgata tgacacaggc tgcatatatt
    56221 gaagatgcaa tgtcgatgtt tgaacaacgt ctaaatgaat tagaagaatt gatcgatcgt
    56281 ttataaggct ttctattttt gattttctcg aatagaaacg aaaaaaatac gtcggctctc
    56341 ttgtaaggga gtcgacgtat ttttt
//
LOCUS       DAFELO010000011        52885 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFELO010000011
VERSION     DAFELO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.011"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LP452_000741"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LP452_000741"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL2013493.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LP452_000742"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LP452_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL2013494.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LP452_000743"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LP452_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL2013495.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LP452_000744"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LP452_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL2013496.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LP452_000745"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LP452_000745"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL2013497.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LP452_000746"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LP452_000746"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL2013498.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQLKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="LP452_000747"
     CDS             complement(8054..8524)
                     /locus_tag="LP452_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL2013499.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="LP452_000748"
     CDS             complement(8570..8995)
                     /locus_tag="LP452_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL2013500.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="LP452_000749"
     CDS             complement(9062..9331)
                     /locus_tag="LP452_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL2013501.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="LP452_000750"
     CDS             complement(9342..10937)
                     /locus_tag="LP452_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2013502.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LP452_000751"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LP452_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL2013503.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="LP452_000752"
     CDS             complement(14490..15050)
                     /locus_tag="LP452_000752"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL2013504.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="LP452_000753"
     CDS             15403..16377
                     /locus_tag="LP452_000753"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL2013505.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="LP452_000754"
     CDS             16547..17947
                     /locus_tag="LP452_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL2013506.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="LP452_000755"
     CDS             complement(18025..18747)
                     /locus_tag="LP452_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2013507.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="LP452_000756"
     CDS             complement(18826..19332)
                     /locus_tag="LP452_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2013508.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="LP452_000757"
     CDS             19455..21254
                     /locus_tag="LP452_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL2013509.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="LP452_000758"
     CDS             complement(21322..22896)
                     /locus_tag="LP452_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL2013510.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERVEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LP452_000759"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LP452_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL2013511.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LP452_000760"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LP452_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL2013512.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="LP452_000761"
     CDS             complement(24673..25311)
                     /locus_tag="LP452_000761"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL2013513.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="LP452_000762"
     CDS             complement(25312..25962)
                     /locus_tag="LP452_000762"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL2013514.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LP452_000763"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LP452_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL2013515.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LP452_000764"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LP452_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL2013516.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="LP452_000765"
     CDS             complement(29105..29845)
                     /locus_tag="LP452_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL2013517.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LP452_000766"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LP452_000766"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL2013518.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="LP452_000767"
     CDS             complement(31216..32154)
                     /locus_tag="LP452_000767"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL2013519.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="LP452_000768"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="LP452_000768"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL2013520.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LP452_000769"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LP452_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL2013521.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="LP452_000770"
     CDS             complement(35314..35619)
                     /locus_tag="LP452_000770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL2013522.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LP452_000771"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LP452_000771"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL2013523.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37009)
                     /locus_tag="LP452_000772"
     CDS             complement(36725..37009)
                     /locus_tag="LP452_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013524.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="LP452_000773"
     CDS             complement(37027..37917)
                     /locus_tag="LP452_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL2013525.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="LP452_000774"
     CDS             complement(37937..38155)
                     /locus_tag="LP452_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL2013526.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="LP452_000775"
     CDS             complement(38125..39825)
                     /locus_tag="LP452_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL2013527.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="LP452_000776"
     CDS             40091..40864
                     /locus_tag="LP452_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013528.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="LP452_000777"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="LP452_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL2013529.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="LP452_000778"
     CDS             complement(41688..42659)
                     /locus_tag="LP452_000778"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL2013530.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="LP452_000779"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="LP452_000779"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL2013531.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="LP452_000780"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="LP452_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL2013532.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="LP452_000781"
     CDS             complement(45614..46438)
                     /locus_tag="LP452_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2013533.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="LP452_000782"
     CDS             complement(46411..47091)
                     /locus_tag="LP452_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2013534.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="LP452_000783"
     CDS             complement(47114..48052)
                     /locus_tag="LP452_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL2013535.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="LP452_000784"
     CDS             complement(48297..49160)
                     /locus_tag="LP452_000784"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL2013536.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="LP452_000785"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="LP452_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL2013537.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="LP452_000786"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="LP452_000786"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL2013538.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT        14662 a        11481 c         9506 g        17236 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt cagttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaacac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatcct gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc acccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttagtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg attatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcgcgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg tgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt
    39901 ccataagaaa acaattcata aaacatcctt taaataaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaagaattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taacagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtaccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctctt
//
LOCUS       DAFELO010000012        49474 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFELO010000012
VERSION     DAFELO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.012"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="LP452_000787"
     CDS             131..505
                     /locus_tag="LP452_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2013539.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LP452_000788"
     CDS             complement(619..1248)
                     /locus_tag="LP452_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2013540.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LP452_000789"
     tRNA            complement(1382..1454)
                     /locus_tag="LP452_000789"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LP452_000790"
     tRNA            1668..1741
                     /locus_tag="LP452_000790"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LP452_000791"
     CDS             complement(1879..2043)
                     /locus_tag="LP452_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013541.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LP452_000792"
     CDS             2338..3666
                     /locus_tag="LP452_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL2013542.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LP452_000793"
     CDS             3868..4701
                     /locus_tag="LP452_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL2013543.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LP452_000794"
     CDS             4717..5454
                     /locus_tag="LP452_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL2013544.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LP452_000795"
     CDS             complement(5609..6577)
                     /locus_tag="LP452_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL2013545.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LP452_000796"
     CDS             6809..7642
                     /locus_tag="LP452_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL2013546.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LP452_000797"
     CDS             7676..8515
                     /locus_tag="LP452_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2013547.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LP452_000798"
     CDS             8541..9101
                     /locus_tag="LP452_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2013548.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LP452_000799"
     CDS             9300..11021
                     /locus_tag="LP452_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL2013549.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LP452_000800"
     CDS             11186..12328
                     /locus_tag="LP452_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2013550.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LP452_000801"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LP452_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL2013551.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="LP452_000802"
     CDS             14128..14775
                     /locus_tag="LP452_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL2013552.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="LP452_000803"
     CDS             15168..17813
                     /locus_tag="LP452_000803"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL2013553.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="LP452_000804"
     CDS             18123..19436
                     /locus_tag="LP452_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL2013554.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="LP452_000805"
     CDS             19426..20079
                     /locus_tag="LP452_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2013555.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20805
                     /gene="radC"
                     /locus_tag="LP452_000806"
     CDS             20134..20805
                     /gene="radC"
                     /locus_tag="LP452_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL2013556.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(20700..22052)
                     /locus_tag="LP452_000807"
     CDS             complement(20700..22052)
                     /locus_tag="LP452_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2013557.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLAEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(22118..23407)
                     /locus_tag="LP452_000808"
     CDS             complement(22118..23407)
                     /locus_tag="LP452_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL2013558.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(23494..24198)
                     /locus_tag="LP452_000809"
     CDS             complement(23494..24198)
                     /locus_tag="LP452_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL2013559.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            24398..24664
                     /locus_tag="LP452_000810"
     CDS             24398..24664
                     /locus_tag="LP452_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2013560.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            24677..24835
                     /locus_tag="LP452_000811"
     CDS             24677..24835
                     /locus_tag="LP452_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013561.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(24832..25062)
                     /locus_tag="LP452_000812"
     CDS             complement(24832..25062)
                     /locus_tag="LP452_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2013562.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            25134..25289
                     /locus_tag="LP452_000813"
     CDS             25134..25289
                     /locus_tag="LP452_000813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013563.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            25446..25601
                     /locus_tag="LP452_000814"
     CDS             25446..25601
                     /locus_tag="LP452_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013564.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(25598..25822)
                     /locus_tag="LP452_000815"
     CDS             complement(25598..25822)
                     /locus_tag="LP452_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2013565.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            26089..26424
                     /locus_tag="LP452_000816"
     CDS             26089..26424
                     /locus_tag="LP452_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013566.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            26657..27598
                     /locus_tag="LP452_000817"
     CDS             26657..27598
                     /locus_tag="LP452_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL2013567.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            27600..28490
                     /locus_tag="LP452_000818"
     CDS             27600..28490
                     /locus_tag="LP452_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL2013568.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            28533..29333
                     /locus_tag="LP452_000819"
     CDS             28533..29333
                     /locus_tag="LP452_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2013569.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            29348..30199
                     /locus_tag="LP452_000820"
     CDS             29348..30199
                     /locus_tag="LP452_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2013570.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            30196..30555
                     /locus_tag="LP452_000821"
     CDS             30196..30555
                     /locus_tag="LP452_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL2013571.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            30570..30731
                     /locus_tag="LP452_000822"
     CDS             30570..30731
                     /locus_tag="LP452_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL2013572.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            30728..31054
                     /locus_tag="LP452_000823"
     CDS             30728..31054
                     /locus_tag="LP452_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013573.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            31051..31866
                     /locus_tag="LP452_000824"
     CDS             31051..31866
                     /locus_tag="LP452_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBL2013574.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            32005..32316
                     /locus_tag="LP452_000825"
     CDS             32005..32316
                     /locus_tag="LP452_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL2013575.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            32313..32543
                     /locus_tag="LP452_000826"
     CDS             32313..32543
                     /locus_tag="LP452_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013576.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            32550..32753
                     /locus_tag="LP452_000827"
     CDS             32550..32753
                     /locus_tag="LP452_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL2013577.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            32750..33049
                     /locus_tag="LP452_000828"
     CDS             32750..33049
                     /locus_tag="LP452_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013578.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            33039..33203
                     /locus_tag="LP452_000829"
     CDS             33039..33203
                     /locus_tag="LP452_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013579.1"
                     /translation="MKFKIFEEDTRYKLEKELNDFAKNNEIQHISLATSKRGYANYYA
                     AVVSYVSREV"
     gene            33292..33705
                     /locus_tag="LP452_000830"
     CDS             33292..33705
                     /locus_tag="LP452_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL2013580.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            33718..34104
                     /locus_tag="LP452_000831"
     CDS             33718..34104
                     /locus_tag="LP452_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013581.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            34655..34879
                     /locus_tag="LP452_000832"
     CDS             34655..34879
                     /locus_tag="LP452_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013582.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            34897..35325
                     /locus_tag="LP452_000833"
     CDS             34897..35325
                     /locus_tag="LP452_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013583.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            35303..36721
                     /gene="terL"
                     /locus_tag="LP452_000834"
     CDS             35303..36721
                     /gene="terL"
                     /locus_tag="LP452_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL2013584.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            36733..38268
                     /locus_tag="LP452_000835"
     CDS             36733..38268
                     /locus_tag="LP452_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2013585.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            38348..39226
                     /locus_tag="LP452_000836"
     CDS             38348..39226
                     /locus_tag="LP452_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL2013586.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            39228..39545
                     /locus_tag="LP452_000837"
     CDS             39228..39545
                     /locus_tag="LP452_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013587.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            39672..40295
                     /locus_tag="LP452_000838"
     CDS             39672..40295
                     /locus_tag="LP452_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL2013588.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            40310..41200
                     /locus_tag="LP452_000839"
     CDS             40310..41200
                     /locus_tag="LP452_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL2013589.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            41223..41438
                     /locus_tag="LP452_000840"
     CDS             41223..41438
                     /locus_tag="LP452_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013590.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            41450..41791
                     /locus_tag="LP452_000841"
     CDS             41450..41791
                     /locus_tag="LP452_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013591.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            41791..42162
                     /locus_tag="LP452_000842"
     CDS             41791..42162
                     /locus_tag="LP452_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013592.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            42155..42550
                     /locus_tag="LP452_000843"
     CDS             42155..42550
                     /locus_tag="LP452_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL2013593.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            42552..42929
                     /locus_tag="LP452_000844"
     CDS             42552..42929
                     /locus_tag="LP452_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013594.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            42930..43538
                     /locus_tag="LP452_000845"
     CDS             42930..43538
                     /locus_tag="LP452_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2013595.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            43538..43879
                     /locus_tag="LP452_000846"
     CDS             43538..43879
                     /locus_tag="LP452_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013596.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            44121..46910
                     /locus_tag="LP452_000847"
     CDS             44121..46910
                     /locus_tag="LP452_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL2013597.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            46900..47640
                     /locus_tag="LP452_000848"
     CDS             46900..47640
                     /locus_tag="LP452_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2013598.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            47637..>49474
                     /locus_tag="LP452_000849"
     CDS             47637..>49474
                     /locus_tag="LP452_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2013599.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT        17093 a         7761 c        10176 g        14444 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaattattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tccggaatat ataagttagt acacataaat
    20701 tatataagga aatttactgt tatattgcca tctggaaata ctattatgtt atcgataatt
    20761 gaacgccata atgcattttt ttcttcttcc gaagattctt tataaatttc atcgaattct
    20821 tttgatagaa taaattcgac attttttata tctattttag ctggctctac ttcaatttca
    20881 tttaattctt tcattaaagc ttctcgatct tttttgtatt ccgctaagtc tataacttca
    20941 ttcaagtagg ctttcttcaa tctatctaat ttattctcaa tatttttctt actatttttc
    21001 tttttaataa tctttggttg tttttttgaa tagtttattt gtatttgttc aaatttttgt
    21061 ttagtagctt caaaaatata acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg
    21121 caacgtaatt tattatttct tcgttttaca catctataac aagtcctttc gtatatagat
    21181 tctgtaccgt cttttttctt atatcttgct ttaatagttt gaccagctac tgatccctga
    21241 cagtcagaac attttaataa acctttaaaa atgtaatcac gttttgcatt tgtttttaaa
    21301 tttttaggta aggctttttg aacagcataa aaaagttttt tatcaataat aggaggacag
    21361 taatttttat tatttcttaa ttcaccaatg tattttttgt tcgtcaacat atttttaaca
    21421 ctttgataat ctctaacgat tccatactga gagcctaaat aataaacagt ttttcttaaa
    21481 ctaccactag aaagaaagta gttgaatata tctatcacga taggagcatc atcatttatt
    21541 actaatttct tattttctat agagtagcct aacggagttt tcccgcttac tacttcacct
    21601 tgtgcaattt tatttgccat aacagctttg atgcgttctg aatcaatttg ggcttccaat
    21661 tcagcaaaac tcattacttg tgcaatgaaa gttctgccat acgctgtagt tgtatcataa
    21721 tattgttgag aaacagcatt ccaagaaaca ttgtgttttt ctaaaatttc ttgagtgttt
    21781 aaataatgtc gtagatttct aaaccatcta tctaattttg taaataaaat tagatctatt
    21841 ttattttctt ttacatcttg taatagtttt tgaaattcat cacgatttat tttttgacca
    21901 gaaataccac cgtctacata ttcattaact actttcaaat ccttgtgttg ttcgatatat
    21961 tcatagaggg tttctttttg ttctctaaga ctatctccat gtttggcttg ttgttcagtt
    22021 gatactctca tatataatgc tactcgtttc atgttatctc ctttcgaatg tatgttctta
    22081 tcgttgtaaa agaaaagccc gaaggctaat ctttttacta cacatctgtt aattctaatg
    22141 atcttctgta ttgttcagct tgagctagtt tagtaaaaga tacagttccg ttataatttt
    22201 ctaaaacaac tttttcaatt tcatcaagag atactttaaa gaattctttt cgttgattaa
    22261 ctttattgac ttcgtttttt ctaaaatgcg aatgtagttg attttctaat gttggtgcat
    22321 cttctgaaaa tatcattgca tgaacatcaa attcaaaagg tacagaagcg ctactaagtt
    22381 ccttaattct atccataggt tctaaccgtc tagtcattcc tattttatag acatcttcac
    22441 cgaaagatcc gatattactg atgacataaa catagccagc tctagtatta agttctcttt
    22501 gcaatacatc ttctttgaca gtttctagtt ctcttaattt ctcttccagt tctttaattt
    22561 tatcagcata gagttctttt tcaacatcag cactagcttt ttgcatgtat gccattaact
    22621 tagttatctc tgagttaaac tgtcgttctt ctttatcaat gcgtttcttt tcacgttcta
    22681 tttcacgtct taccttttct tcttcaatca tttgttcttt tattgctttt tgctgttctt
    22741 tttcatcgtt aactttaact atgtatgaat agttgagttc taattcttga agttttaatt
    22801 caagtaattc ttttttgaat tctgcttcat cgtttttaaa aagcttgttt attttatcaa
    22861 aagttcttat aattttttct ctagttgaat ctacgttaga aatagtaagt ttattgagaa
    22921 gtccagttat ttctgattct aatggtataa gcattttctt tttaatatta ttagcttctc
    22981 tattattcaa ataagaagga atattaattg caccgtctaa aataaaatcc ttttctttaa
    23041 tctttaataa agaaagtttg ttttttattt cggatgaaga agtttcatct atattcggaa
    23101 taattactga tttatagata gtgaccagag atgtgtcata tttttgatta atttcatcgt
    23161 ctaaccgctg gctttcagca ttaagaatat caattttgtt ttctagcgtt gacaagtttt
    23221 tattcaattt acttatgttt ttctcttttt cagatataaa cgtttcccta gcatttaatt
    23281 cttgagaata ttcctttacc ttactctcta aggcagaagt tttatctttt aattttttag
    23341 cttcagataa tttgctacta cccataatta ttaaaactat tccaactaaa aacaatagta
    23401 ctcccattca aaacgcctcg cttcttttat aaaataattg tcaaaatcat ataaaccctt
    23461 accgaagttg acggctccgg catgggaatt tttttaaaat acaacttttc cgataattct
    23521 aatctgttct ggcgatacaa ttctatcttc gtataagtcg tttatagaac gtaaaatgat
    23581 tgtttgattt tcgtaatctt tatatatctc tttacatgta actccgtcac cacctaattc
    23641 cacaatagca atttccccat cttctacgtc cggttgttct ttaacgaata cgaattgtcc
    23701 attcctgata acaggttcca ttgaatcgcc tataataggt actgcgaagt cagcagcact
    23761 tggaactctc tcaaattctt cctcttcgac aaccgtatca ccgtaagtaa gttctgttgg
    23821 attggcagcc gaagccgaat tgtgaacagt tgggattttt tcttttttag gaaaatctag
    23881 gacattttct tgtttttgtt cttctaatcg attacttgca aattcatagg tagcaacttt
    23941 attttcgtcg ttaagctgat tatatatagg aaggatatta tatatctgat tagatataga
    24001 taaatcaata ttcatcaaat catctatatc aacattgaat atcttagcta tctcatttaa
    24061 ggtttttaat tttggtgtat attttccttt ttcccattcg cttattgagg atgaactttt
    24121 tcttcctagt ttttcggcta gttcttgttg atcaatatta tacttttctc taagaaactt
    24181 tagattttca gcgaacatag taacacctcc ttgagttaat tgtaacaccg tttcggaaaa
    24241 aatgtaacag gtttcttaga aaatatttta gtgtttttaa agaagtattt acgcaaaata
    24301 gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc ggaaaaaccg aagtatataa
    24361 tttagtcaga aagttaaaga gaggaggcga ataaaatatg gataccgcga agaaagaaaa
    24421 atatacattg agacaattaa gagctttaaa gggttattca aaagaagaac tgtcaaggaa
    24481 aagcaaagtg acatcaagaa caatatttat ttacgaaaac gatgtagata aaatgaggaa
    24541 cggaaagtac gccactctag aaaaaattgc taaggcattg ggtgttagag tttccgatat
    24601 ttttttagac cctgattcgg aaaaaccgaa atcaataaca ggtgaaacaa aacaagtcag
    24661 ctaggaggtg tgaatagtgg aagagaagaa tataaaagta ccgactcttg aagaatatac
    24721 aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca atcgcagtta ttacggcgaa
    24781 tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc cctagatatg attaatgttc
    24841 tttatcacgt ggaggtatag gatcgtttcc aaaactatct tttgattgta tttgtccatt
    24901 ctttttttgg acaataaact ctgattgctg ttttttagaa atctttttac catactcaac
    24961 agcatctgat ttattatcaa atagttttgt atcttcggaa ttaccttcac cacgtacagc
    25021 ccatttgcca tttctaggag atacccattg atttttaccc ataacaattc acctcgcttt
    25081 caagttaatt ctattaagag gtgaaaagaa aacaaactga tggaggtgag attgtgagta
    25141 acaagccaaa aatcactttg aatggcacac ctaatcttca aagattagca gatgaaatgg
    25201 ctagaatacg ttctagacat tggggagtta ctgtggtggc aaccataatt gaacaaaaag
    25261 aaaagcccac acagccggca agcaagtagt gggcaccata gaacaataat attatatcac
    25321 acactcgcca cttccgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac
    25381 gtttcttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
    25441 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
    25501 ttattgaaac agaagaaggg gaaaaagtag cagaagttac attgaccgat gcaactcctg
    25561 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
    25621 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
    25681 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
    25741 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
    25801 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
    25861 tagaaatgga gaaattcaat aaacgaaagg agggatcaaa tgacattaca agagcatcaa
    25921 cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa tgaaacctag attgctaggg
    25981 gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa aaaagtgact cagccgtcca
    26041 aagcaatgag tcacaaacca aaaattatac ctaaggagat tttatcacat gaataagaaa
    26101 attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt ttctattatt
    26161 tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac aacagaaatt
    26221 ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt gtcagcacat
    26281 attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga agaaaatcaa
    26341 ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt gatgactcgt
    26401 attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc attattttat
    26461 ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta ttcgcaatta
    26521 ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa cgagttagca
    26581 aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga agcgccaatt
    26641 gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag aatggctcga
    26701 agaccgcaaa agaggtattg gtggctcaga tgttgcaacc gtacttggat taaacaaata
    26761 caaatctcct tatcaattgt ggcttgagaa aacgggtcaa attgaattga aagatttaga
    26821 aagcgaacca gcttattggg gtaatgtttt agaagaagtg gttgctaaag agtttcagga
    26881 acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat tacatccatt
    26941 tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg aatgtaaaac
    27001 agccaatcaa tttctcggaa aagagtggga aggtgaagag gtaccgctta gctatctctg
    27061 tcaagttcag cattacatga acgttttaaa caaagactat tgttatatcg ctgttttgat
    27121 cggtggacaa aaattcattt ggaagcggat tgaacgagat caagagctga tcgatacaat
    27181 cactgaacaa ttagtagagt tttgggaaac gaacgttctt ggaggtatcg agcctgtaat
    27241 tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa tatgcagatg tagaagaaaa
    27301 tcaaacagct ctaccatcac gttttgatga acttatcgag caaaaaagag aactcaagcg
    27361 gacgaaaaaa gaaattgaat catctattcg tcaagtagac aacgagatca tcagtgagct
    27421 aggtaaacgt gaagccagta tcggtatcac acaaagaaac attatcagct ggaaacttgt
    27481 tcgtacaaga cggatgaaca cgaagaaact agcagagaaa tatccagatg tcgcaaatga
    27541 tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaaaggaga tcaaataata
    27601 tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa gccacagaaa caagttgcac
    27661 caggacagtt agggcttaaa gctctaatga atacaccaac aatgagaaag aaatttgaag
    27721 aagtacttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt gtatctaatg
    27781 acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta actgctgcaa
    27841 cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca ttcaatagta
    27901 aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc tataaaggat
    27961 atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc gaagtttacg
    28021 aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat ccaaatggta
    28081 gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat ggattcaaaa
    28141 aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac aataaagatc
    28201 gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg gcacgaaaaa
    28261 ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg caagaagcca
    28321 ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt gctgaaacag
    28381 aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa gaaaatgaag
    28441 atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa tgagggagct
    28501 ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc aaattacatc
    28561 gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta tggagcttgt
    28621 gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa gagatacaag
    28681 tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg aacgaaggcg
    28741 tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa caatttgaaa
    28801 aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca ataaaaaact
    28861 ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac
    28921 aagtgcacac aaacaagaat gaaaagaatt ataagaatga aaagaataat aaaacatcat
    28981 cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc agtttactac attctcgcgg
    29041 aggagttatt caaacagatt tgtcagaatc aggaaatcaa aaagccgaat ctgcaaagtt
    29101 gggcagataa cattcggaaa atgatcgaga tcgacaagcg gaccgaaaac caggtacggg
    29161 gaatgattga atggagtcag cacaatgtgt tttgggcatc gaatatttta tctgctaaaa
    29221 aattgcgaga aaaatacgac acaatggcag cgcaagcaaa tcgtgattat aaaacaaaac
    29281 aaactaaaac gcttgaatac gagaaattta gcacagatga gttgcctatt tgagaggagg
    29341 cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg atacagaaag tgcttgtcca
    29401 acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga tggcgtacga agaaccctga
    29461 tggctcagaa cgttgtaagc caacctgtat gcaatgtggg tataaagcat tgcgagttca
    29521 ggaagactta caaacagaac gaatttacaa cgagagcctg aaagcaagag caatcaattt
    29581 tttcaaaggt ggttctgttg tgcctaatca agcgttgttt gattgcacat tgcagaatta
    29641 tcaaattgtc gatcaagaaa caagacaagc ggttgaagta accaaacgct ttgttaattc
    29701 agtcttgtta ggaaatccaa gtcaccttgt tttaactgga aaacaaggaa caggtaaaag
    29761 tcacctagcg atggcagcgg cttgggaagt cttgaagcga tcaaactacg ataaaaaaat
    29821 cttatttatc gggttacagg aaatgctgga tcaaatcaaa ttttcgtaca acaatcctga
    29881 actcagaaaa acgattgagg gatcgttgat tgcagatatc aaaacagcgg atttagtcat
    29941 cattgatgat atcggttcag aactaggaaa agatgcatca gatagtcgag cgtttggcat
    30001 aaacacgcta aattcgttct tggatgcacg acagaaccta gcaacgatta tcacaacgaa
    30061 cttgcttggt gaagaactga aaaaagctta cggtacgaga acaatatcaa gaatgtttgt
    30121 caactctgat ggatttacga tggtattttc tcaaacagca gacaagcgca taaaaccagt
    30181 gaaaggtagt atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg aggtatcaag
    30241 tttgattcta tcgcggaagc agagtattac gatctagcct tgtggcaagc tgaagcgaat
    30301 ggttggaaag tgaagctcca ggaaaggttt gagctgatgc cgaagtttga actagagggc
    30361 aagaagtatc gcaagatcga gtatattccc gacttcacat tttacaaaag cggcaagctg
    30421 gtcaaagtcg tagatgttaa ggggatgcag acaaaagact ttaagatcaa ggcaaagttg
    30481 ttctgtcatc aatatcaagt gccgttgata ttagccaaaa aatatcggaa tacgttcaag
    30541 gaagagcgtt tttaacgagg tggtccatca tgacaacaga agaagtgatt caaatgcgaa
    30601 ttcgaagcct tcggcgtgag attgacgatc tggaacgaac aaaggcagtg atggtcaatg
    30661 aaacggctag aaaggcaatc gatttgcaca tagagaattt aagaagggaa atccgtagat
    30721 tggaggaatg agcgtggata aggaagcggc ttggcgaaaa ttaatgttgc tgattcaaga
    30781 tgagaactgg caagaagatg aagcagtggt tgctgaagtt cagcgtctag gaaagattgc
    30841 taacggacgt atacgaaaaa agccagacaa aagaaaacag cgcaaaggga aaatcgtcgt
    30901 tgttttacac gaaggcaaaa ttttgatgca aggaacagct agtgagctgt ctgcagaaac
    30961 tggatatacg cgtgggacta ttcgaacgta cgcttggcga aatcacaccg atcgaaaagg
    31021 gcacgaatat aagtatttgg aggaagaaaa atgaacgaaa acaaattaat caaattgggt
    31081 gtagcaggag cagtaatcgt aggtattgga gttatcggag gatttaagtt cttcgaaaaa
    31141 atcgataatg gatatgtggg tgtgcgctat tcaatgaacg gcggtatcaa agatgaagca
    31201 ctgacgcaag gtgtgaaatt tgtagggatt gacaaagtga tccaatatcc aattcgcttg
    31261 caaactatcc aatcaaaaaa tatttcagta tctacaagcg acggcaaaaa gacaacgatt
    31321 gatatcaaat atgactacaa agttgattca actaaagcag caaaaatgta caaggaattt
    31381 gggaatatca cttcggaaga tatcgaaagt ggatggttaa aatctaagct tcaaaaggtc
    31441 gctcgtgaag tttatgcgaa atatagtctg cttgatgtcc tttcaggaga ttcctctaaa
    31501 gttgaagctg aggtattaac gaactttgct aaatcagttg aatctaaagg gtttgaagtc
    31561 gaagacgtaa cacttggtgt tccagatgtc gataaagaaa cacaaaaatc aatcgatgcg
    31621 atcattcgag ctggtcaaga aaatgaaaaa gcgaagctag acgcagaaac tgcaaaaact
    31681 caagctgata gtgaagctta caagaaaaca aaagctgcag aagcagaggc agaatctaat
    31741 cgcaaagtcg ccgaatcagt aacagacaat ttgattcgtt atgaagaagc tcaagctcgc
    31801 aaaaagcatg gatgggtaac agtaaatgga gcagatactg tagttacgga tgaagcaggc
    31861 aaataatatg ggattcttta tggctaaaat tctcttgttc ttaggtttag taggagcagc
    31921 atatctcgtg tatgcgctcc tttcccaaac tgatgacaaa gaagatgaca acaacgatga
    31981 aatgaaattt taggaggaga aataatggac gaactaatca caaaagtaga gcagtgggct
    32041 aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt agctgaggaa
    32101 ttcggggaaa tagcttcggc gatggcaaga agtaatgatg aactatttaa agatagcgta
    32161 ggagacgtta tcgtcacgct gattatcctt tccatgcaaa aagggacaaa cgtacaagag
    32221 tgtttagaaa tggcatacaa cgaaatcaaa ggacgcacag ggaaaatggt agatggtgta
    32281 ttcgtgaagt cgagtgattt ggaggatgtg aaatgaaata cgaaatacca ctaagtgaag
    32341 cgggcattca agcaattatc aatggtcggg aggttaacat agaacttcct gatggtactg
    32401 aattagtcat cagacaaagt tatttgaaag atatggcagc tccagtatta attgatcgtt
    32461 ttaacgtgac tgattctgtg gtagagaacc acttaaaaga atttcgatca agtatagacg
    32521 acactttcag attagggagt tgattgacaa tgaacaacag acatcgcaga ataacaaaac
    32581 taagaaaaca ggaactgaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
    32641 cagaagaaac atataaagtg gcaagtcagt ttgttgctga tgcaagtgat gctattcgta
    32701 agtttgggat ttcgatatta aatgatgatc gtaaatggga ggaaatgaga tgaaactaaa
    32761 agacggattt tacgctagta gtcacggcat cggcggttta atgctagata tgccgacaaa
    32821 gaaccctaaa acacgtaaga aaccaaaagt caaagtcggt gacatggttc gctgtgaagc
    32881 agaagagttc atttatccgt ttcgtggata tgtagaacac gtctataatc actcagcgat
    32941 cattcgtatt gaaaacacaa tggaatgcga taagtggtta gcgaaaagca aagagaattt
    33001 agctgtagcg agattggtgg atatggaggt tataaacaat gaagtttaaa atctttgaag
    33061 aggacactcg ttataaatta gaaaaagaat taaacgattt tgcgaaaaac aatgagattc
    33121 agcatatatc tttagcaact tctaagagag gttatgcaaa ttactatgca gctgttgtga
    33181 gctatgtaag tcgagaagtg taactcggca aataaaaaaa gccggatcgc tccgactgat
    33241 tcaataaatc caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg
    33301 ttacgagagg tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt
    33361 cggcgtttgg agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg
    33421 gatatgccga aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcaaatgatg
    33481 gatatagaag cggagagaga cgcgattcta gtggctttga tggctcttag tctgattagt
    33541 cgtcagatac tctactacag cttctgtgtg ccagacagct tctcaaacta cagaattagc
    33601 cgtgaagtgg gttattcaga aagaagtata caacggatga aatcggaagc tctaatagag
    33661 tttgcagaag catataaaca cggaagaata attgcttata aataggggga ccatataatg
    33721 tggaaagatt atgtatcgtt gaaagagttg aaaaaagatc ttgttttcaa aagaattgta
    33781 gaatggtcag agagtgaatt aattttagaa gatggaacca aaatggaagt tgtatgtagc
    33841 gaatcagatt gctgcgcatg ggccgaaggt gaattcaaaa atgtaaagct tgatgcagtg
    33901 attacagata taaagatttt tgataaaggt aaccgtctct ataatggtga cggacatacc
    33961 tcttatgctg aagtcgttgt ttatcataat cgtaatgaga tcgctaaagc agaatgcaca
    34021 gcgaatgatg ggaatggcgg ctactattat agtgtttgtg ctttaaaggt caaagacaaa
    34081 ctttgtatag tgactgacgc ataaaaaaat ggcggttttt tggcggaatg atggcggttt
    34141 ttagccattt atcagtgata ttatggtagt gtcgaaagat tagtgatagg tctgagacaa
    34201 aataataata aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc
    34261 agcggaaata ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc
    34321 tatcatccgt tgctgtctat tgttacatac agatcgctta ggcggtcttt ttattttggc
    34381 cgaaaaccta cattttcgat ggccgattat tggaggagga atagcatgtt caaactatct
    34441 gaaatcatta agaagtccga cgttgagaaa ttagagatgt taaaaaagaa gttgaagaaa
    34501 tagcagtctc ttcgtgaggc tgttttattt tgctcacaaa aatagaccac tatcgggtaa
    34561 tagtggtcag gaattaaatg aaaaagatgt tagggttgtt agctaagtat aacatcataa
    34621 cgcttacaaa acaatgcaag aaggataaat tattatgaga acttactggt atgtgtcatt
    34681 aaacaataaa tatccgaagc caatgaaagg acagcatagg cgtgtagtca tgtctgttca
    34741 aatgaaggcg aagtattcga ttgtagaaat gatcagagag gcaacgccag tagagattga
    34801 ttattgcaag ctagtctatt gtgggtgcgg ccgttggaaa gaggatcatg tacaaaaaaa
    34861 tatcagcaag tacatataga gaggcggtga ttaaggatgg aaaaaagcga tacgaaagcg
    34921 aacagataca aacctactgc tgcagagaaa aaactcttgg aagtattgat aaatccagat
    34981 aacttaggca aaccggtgca agagttgtgt tcactggcga aaataggtag gaccaaatat
    35041 tacgacgcca tgagcaaaga tggctttgtt gatctagtta acgaaacaac aatggatctg
    35101 atcaaaggaa aagcttctga tgtccttaat gccacttaca agtatgcatt gactgaaaaa
    35161 ggtcatcagg ataggaaaat gattctgact attgctggaa tctatgcaga caaacaagaa
    35221 ctcaaacatt ctggcggcgt cgatatccga aaacagtatg agaaaatgtc cgatgaagaa
    35281 ttagaagagt tggtgaaacg gtatgaaaaa atcaacgact cttaatcgtg ttgagtatgc
    35341 cgttgttcaa agagagctag ccattcgaaa agctaggaaa agtttttggc tattctgcca
    35401 gcttctttat ccagagtttt acaaagatga tcggttgtat ttgaaagatt tatgtcatac
    35461 gctacaagat ttttacgaag ataaaatcga caaaaaaatt ttggttatta acatgccgcc
    35521 tcgacatggg aagactttta cagcgcggct attcgtttta tggatgtttg gacaaaatcc
    35581 aaagacgaaa attattaccg gatcttataa ccaaatcctg tcatctttat ttgcgcaaca
    35641 aacacgtgat ggaatcatgg ttgaaaacga aaatgttaag gctgaatact ttaaagatat
    35701 ttttccagag gtgcaaatca aatatggaga tgctgcaaag ggtttctggt ctttagatgg
    35761 ttcagaagaa aagaattatt tagcgacttc tcctggtgga acatccactg gtattggcgc
    35821 gaattatgta attgttgatg acatcattaa aaccgcagaa gaagcctata acgagcgtgt
    35881 tttagattct cactgggaat ggtataacaa cacgttggcc caacgaatgg aacggccacg
    35941 taaacagata ttgattatga cacgttggtc aagtaatgat ttagccggaa aaatgttgac
    36001 gagacgaaaa aataatgttc atcagatctg ttataaagca gtacaagaag acggctcgat
    36061 gttgtgcaac gagattatga cccacgatga gtacttagat gtggtacaag aaatgaatgt
    36121 agatattgca gaagccaact atcagcaaga accaattgac caaaaaggca ggctatatca
    36181 gaagtttctt acttatgata cgttgcctga taacattatc aaaatatgga actacaccga
    36241 caccgccgac aagggtgccg attactttgc gtctcctgtt tttgctgaga cgtctgatca
    36301 tgatgcttat attatcgatg tcatgtatac aaaagagccg atggaaacaa ctgaaatagc
    36361 ccacgctaat atgattattc gcaatcgagt taataaggta cggattgaag gaaacaatgg
    36421 cgggcgtgga ttcagaagaa attcagaaag aattacaaaa gagcgaggct atcgaggtgc
    36481 agtatttgaa gactttcatc aatcggataa taaactgtct agaatactgg cgaatagtgc
    36541 ttgggtggaa aatcatgttt actatccaga tgattgggcg acacgttggc cggactttta
    36601 tgaggctatg gttagttatc aacgtgaagg taagaacgct catgatgatg ctccggatgc
    36661 agttacagga attgcagaaa cgattataga agcagatgac ttcgagttta ttgcttatta
    36721 ggaggtgggt ctttgtttta ttctggaatc acattgaaaa aatacaaacg tttaagaacg
    36781 aaatattcaa cgcaaatcga agaaggatta ttcgatccta acgcttttat ggaagatatg
    36841 aaaccctttt tcgatgatcg agaaagaaag tatcttgctt atactagcga gaaaaacgag
    36901 atcgacagtc gtgagaaacc aaaaacaaaa atcattaagg tcaataacaa gattcatgcg
    36961 ggaatgtatt cagtggtcgt tgatcaagca gttaaccatt tcacaggaat accaattaaa
    37021 tgggattatg atgtttcgga acagaaaagg acattcattc aacgaatgag agacaaatta
    37081 ctgggtaatg atatcagcgc cccttctacc ccagaagaat ttgaaacact cacgagacaa
    37141 ttaagtacta tgcggtttgg aatgttagac cccgatactg caacatttca aggagcgaca
    37201 gggcttgctt tcagattact ggagcctgtt gagacaaatg atggttggga actaagagca
    37261 agcaacattg agccgtggaa agctgaaaag tacgaaaacg gcgcagtgta cattaaagag
    37321 aaatacgatc catatcaaaa gaagttttat gaagagatga aggttgtcac gccgaaatca
    37381 atatttactt atgctcgata tactgattac cctggcacgg tacaaggagc atttaaaacg
    37441 ctgggtaatg ttcctaatcc tttaggcgtt atcaatctta cggagtttaa aaacaataca
    37501 aaccgttact gtgactttga agttgcagag gagattagtg atgctattga tcgtgtttta
    37561 tctgatcagc agaacgagat tgaacaattc aagttagctt atatgctgat cactggggca
    37621 gatttgtccg aggataaagc caagaagatg atggagcaac tagggatcat caatctgaaa
    37681 gaccctaatg cgaaagccgg ctacgtgaca aaggatctac aaaaggattt caacgagtat
    37741 cattttgatc ttcttaaaaa gcaattctac acggtgtgta aagcaattga tttcaatgac
    37801 gaagtcttta agtcgaatag ttctggggaa gcccgaaagt ggcaaatcat tagtttagaa
    37861 gctaagacaa ataccaaaga acagtacttt cgagaaggat tgaaagagtg tgccgaggtt
    37921 atggctgctt atttggattt tgccgagaaa gttacaattg atccggaaaa gatcattttc
    37981 accttctctc gttcgcttcc aacagattta agctacctag ctgaggcatt gcctaagttg
    38041 gcaccatacg tttctaagag gacgattcaa agtcagatcc cgttcgtcac agatgttgat
    38101 tatgaaaacg aaatgattga cgtcgaaagc agacaatctt acccagacgg cgagtatgga
    38161 ggtggcatcg ttgagtcaaa tagaaacgaa gtactggacc aaacgtcgag aactggaaga
    38221 tttagcaaga ctcaaaatgg aaaatcaaac gctaaagaaa ctaagtagtg tctttcctga
    38281 tgcgttgaaa gaaattcaag cggagttatt atcccaagct gatttacacg atattaccta
    38341 tgctgagatg atggaatttt atagtacaag tgatcagaaa aaatatcgtg aatacgttga
    38401 aaagaactac aaatcactga agatgtatga tgcaaaatac aaagagttca tcgaagaata
    38461 ctttccttct ttcgactacg ccaaagtgaa tcggctgctt caaatgaggt cagatatttt
    38521 tcgaattctt gcagagagtg caatagatgc agatgtgaat aaatattttt cggatcggtt
    38581 agaagatatc ttgcaacgtt cgtatgtatc aaacgcaaac gtatttgccc agattttgaa
    38641 cgtagatttg ccgaattact tatcaaagga agaacttgaa cgttacctga actatccttg
    38701 gtcaggtaag acattctcgc gtcgtttatg gggcaatatt tcatctctag aacaaaagct
    38761 atctaacgcc attacaacta gtgtagcaag tggcgaagga gttctcgtgg cattacgaac
    38821 aatgcgagaa gacccagaaa tatgcgatat gttcaaaagg gaggaatcga agtacaacaa
    38881 ggcgatcgaa aatcttgtga gaacagaata cgctaaattc gctcaagatg gtattgagaa
    38941 atcttatatt gaaactggcg tagacgaata caatgtctta actgcaaaag atgaaaaggt
    39001 atgttcaata tgcggtggca aagcaaagaa caacccatac aagttgagtg aagcgatcat
    39061 tggtgagaac cgtgcgcctt ttcatggccg atgcagatgt acagatgttc ctaatatgcc
    39121 gaaattagga aaagatattg acgaagagta tgagcgttta tttggcgatt tattagatga
    39181 gtttgcacat gattcttttg gaattaattt gaaacggagg aagtagaatg aaagattttt
    39241 ttgaagcagt actaacaatt aatgtaaatg ctgatattgc agaagcctac aaaacagcta
    39301 ttgagtctga gaaccatcct aatggcttga gagaccattg gaatggcaat tatgcctacg
    39361 tggttattgg cgatcaaact gttaattatc aagataatac tccagttgat aagaataccg
    39421 ttaatttaac gattcaatta ttatctcatt cattaccaaa tttaaaagaa acagttgatt
    39481 ggtatgaaaa tatgggatgt attgttgtta gaactgacta caaagaagga aagtctagta
    39541 attaggcttt tttattttgt ccgaaatgac gttaaactag cgcaatactg ggcttggttg
    39601 aatggtgggg cgcaactatt aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg
    39661 gcgaaaggag aatgattatg aaacacaaat cattaatgcc aatgaatttg caatactttg
    39721 ccgaaggtga cgatcaaaag ttttcttttg atgacttcaa atcttttgtg gaatccaatg
    39781 aagaagctca aaaatttatt cagtcacaat ctcaaagtgt tgccgacaaa caattggaag
    39841 cttggaaaca aaataactta gataaaatta aacaggatac catcaaggaa tatgaggaat
    39901 ctaagaaaaa caagtcacct gagcaaattc aactggaaaa actacaagct gaatttgaag
    39961 cagaaaaagc gttgcgtgtg acaagtgata ataaagcatt tgttgcagaa caaattgctg
    40021 gattagaact agatggagag ttaaaagagt ctatttctca atttatgcta aataatcttg
    40081 ttagttcgga tacagatttc actaagaatg ctgttgaagg ttttacaagt gttttgaatg
    40141 caattaatga gaaacatgca gatgcattaa aagaactaca aatgaagtct gcatttggtg
    40201 gaactcaaca atcgaataac caggttcagc agaacaatga aacattaaca aatccagaag
    40261 aacaattagg acaaattctt caacaattta actaggagag tgaaaaatta tgaaaaaaac
    40321 atctttaaat aatttagagt atttggatat ttcaccagcg attaatgcta tgcaagtacc
    40381 aaatacacct tttttaagct atttacttgg tgctggaaaa acagagccag caaactcgac
    40441 agaaattaaa tggcgtgaat atgatatcaa caacgatgat tcttctgaaa aacttgaggg
    40501 cggagaatat ccagatgctg aatcaggtcg aacttggttt aacaactata ctgaaatttt
    40561 tagaaaatca acctctgtat ctggtacatt agatgctatt aatgtgaatg gagtcggaaa
    40621 tgaattaact aatcaagtag cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa
    40681 attgattact ggtgtaaaag ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat
    40741 tttgaacttg atcaattcag caaataaggc agaaacagcc actgcgggtg cagtaacaag
    40801 aaaagatatc gatactttat ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact
    40861 atgcttgatt tctccagata tgcaggagtt aatgactgat gagttagatg gaaaatcaac
    40921 aaaaattgtt cagttcgggg aaagagtaac ttttggattg caaattggaa atatcgtgtc
    40981 taattacggt acaggtattg ctctactaga accatcattg ccaaaaggaa caattgccgc
    41041 aatcgatact aattatgtga aactacgtcc attacgtgaa tggagagcag aagaacttgc
    41101 aaaaacaacg gattctagac gtatcggtct tgtaggtgaa tactctcttg aatacaatgc
    41161 ttcaaactct ggggcaattt taaatttaaa gtctgaataa aagggagtta gtactccctt
    41221 ttttgatagg aggaattaca gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga
    41281 aatatagagt aggtaaaact aaaaattttg ttgggtttgt tcatcctaaa actcgtagat
    41341 ttatcacagc agattcaaat aacgaattta tcatttctat agatgataaa gaagcaattg
    41401 caattttgga agatgcaatt gatgttaatg aaatttagga agtgatctga tggatgaatc
    41461 gctaaaaacg gaaatcattg agtctacaaa agaagatttt ccagatttga gtgaagaacg
    41521 cataactaat ttattagaaa taattttgct agaaattgaa tcatacaaca cttgtaaaaa
    41581 tgatatttca tgggaaaagt taaaaagcgt gattagtgaa gtgttgtata aaataataaa
    41641 aaatgaatca gaaaaaacag tatcttcagt tagacgtggc gatacaacga ttagttatgc
    41701 ttcaacaaca aatgatgtta gtgagttact tctaggctat ggtgatttga tacgaagagt
    41761 tattggttgt ggaggattgg agttttttta atgaatgaag cagatatttt agaaactact
    41821 tatgaagata gttgtattat tgaaagactg acggacattg aagattctaa tacaaatatt
    41881 actattcaag attacaaaaa agtatatgat aatcctattt cttgcgctct ttcacaaggt
    41941 caaatcgatg gactagcagt catagaagat ggagagatgg taaatgtttc aactgacaca
    42001 tataaattat ttgttcatcc taagattaaa ctaaaaaaag gagatcgaat aacaataact
    42061 caaaaggcca gtggcttaat tttttctcta tttgctacta agccttttta ctatcctagt
    42121 cattgtgaag tgaatttgat aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa
    42181 tgcagaaaag atcattgaga attttaaaaa tatgactgta attgctcaaa aagaaggaat
    42241 atccttcgtt aacgattcaa tgaataaagt cgttagttta gctaaaccat taactcctgt
    42301 aaaatcaggt aatttaaggc gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg
    42361 tcgaattgtt ggagcagtta taaacaatga acattatttt aaatatgtag aagaaggtca
    42421 taggactaaa aatggtggct ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct
    42481 tgcaaatatg tcttatattc ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa
    42541 aggaaagtaa catgaaagat gaaatcattg ctgcaatcag tagcaaatta aaagaaatct
    42601 atcctgatgg gacaatatat cttgattcag ttatgcagtc aactaaagat ttttatttcg
    42661 ttttatcagt aatggaatct ggaactgaaa atgtaggaat tgatattcaa aatgtttctt
    42721 tcttaattga tattgcattg attgataata aacctaatag aaatttaatt aatgaattag
    42781 tctcacgttg tgggactttt tttaatacga ttacaatcga tgagcaaata ttatttccaa
    42841 aagcctattt acctgatgaa gcagatggtg ttcaacatat tcgttttaca ttagaatttc
    42901 cacaatatat tgaatggagt gaaagataaa tgggagaaaa aagaagtaaa accggaatta
    42961 tttctgtcga aaaacctact tggtttcctt tggaagatga gacagggaat ttcccggtat
    43021 atggtactgc gttgccaatg ggaacagcag taagtatcaa accaacagct aattatgaaa
    43081 caacacaaga ttatggggat tcagtggttc aagatcaatt tacagctttt ggtggtgcag
    43141 aagttgaact ggaagcaaat ggatatactc ataaagtttt atctacaatt actggaggga
    43201 aaattgttaa aggtggtgcc ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg
    43261 catatagacg taaaaaatcg aatgggaaat accgttatac ggtcttttat aaaggacagt
    43321 ttgctctaga ttcagatgaa acgtcaacga ttgaaggaag taaagtaagt tttactcatc
    43381 caacttggaa gggttcattt gtagatgtac caggacttgg atatatgtat tcagttgatg
    43441 aagatgatga aggcgtggat aaagcaatga tcggaaattg gtttacaaaa gtagcaattc
    43501 caattgaaga agcagaactt tcaggaggta cagaataatg tcaaaatatc aaacaacgat
    43561 taaattaacg aaaaaagatg aagaaggtaa atatgagcaa gtacaattta aatctgctga
    43621 atttttacca ggaacagttg tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc
    43681 gactgataaa caatcagtta aaaaagcttt aagtcgtgct tattcattta ttgcagatac
    43741 tttatttgag ggacaattta ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat
    43801 tgcctcattg acaggaaaat tattgaagtc tgttactgca ggttttgatg aaacttatac
    43861 agagacgaaa aaaaagtaag tgaggcgctc aagtcacctt catttaagta ttcgattact
    43921 taccgagaat tagatataaa gacgcaatta ctcgaagcag gttggacgtt accagaaatt
    43981 gaacgtactg acttggatga gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag
    44041 catgaagatg ttgagtacta tgataatttc actcaatttt aggaggtgat actttgaata
    44101 acgatgacct aattctgaag atgatactag atgaatctgg ttttactgcc ggtatgaata
    44161 atgctgtcaa aaagttgaac tcttttgatg aaacaatcga aagaaaaagt agaaatagtg
    44221 gcagttcatt aggtagtatt tggaaaatat ttgctggaag ttttctagct agcggagtaa
    44281 ctagaatcgt aggagctggc tttgatctaa tcaagggttc cataagtgga gcggttgatc
    44341 gagtagatac gatgaataac gctctacgaa atttccaaaa tatgggattt agtaattcag
    44401 aaattatgaa gaatatcgga aagaatgggc ttttatctca aggtattcaa ggacttccta
    44461 ctgctttgaa tgatgcgata agtcatgttc aacttcttgc ttcttctaca ggtgatttaa
    44521 ctcgttcaac tcaaatattt aaagctttaa atgatggaat tcttggtttc ggtggttcaa
    44581 ctgatcaagt taatgaagct gttattcaat tatctcaaag tttctcaaac ggaaaagtag
    44641 acgcacaaac ttggaactca atgatcaacg ctcaacttgg acctactctt tctgctattg
    44701 ctaaaaagat gggaattaca atgggagatc tgaaagaagg tttgtctcaa ggtaagattt
    44761 ctgttgaaga attccaaaat caattaatag aaatggatac caaaggtggc ggaggactta
    44821 aatcattaag tcaaatcgct aaagattcta ctaagggaat taaaacctct atacaaaatg
    44881 caaaaacagc tgttacacgt ggtgttggtg aggtaataga aggattaaat aaagcattag
    44941 tagattcaga tttaggtgga tttaagggaa ttattgataa agtgggcagt tcaatggaat
    45001 cgttcttaaa agtaattgct gcaaatattc ctacagcagt atcattttta agtaatttat
    45061 ttaacgaaat tcaaaaattt ggttctgctt tgaaattcat gatgcccttt cttgttcctg
    45121 cagctaccgc ttttggagcg ttcatgtttc aacttaaagg tataccagca ataataaaaa
    45181 gcttcaataa ttttaagaat gccataattg gcgttggaaa ttcattaaag attatggggg
    45241 caatagctgc tgctaattca tttgttttaa ttattggtgc agtcgttggt gcaatagctg
    45301 ttttcggtta ctttatggca accaatgaag agtttagaaa caaggttata tctatttgga
    45361 atgatgtgaa agattccgta attggtgtat taaaaaatat aaaggactgg ggaattgata
    45421 cttggaattc tgctaaagaa atggcatcaa atgcagttga gggtgtcaaa gatgcttggt
    45481 caggaacaaa ggaatggttt tctaacacct ggaaaggtat taaggatggg gccacaggtt
    45541 tatttgataa aacagtagag acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt
    45601 ctaatatgaa gaaatggttt tctgatactt ggcaaagtat aaaagattca gccagagaaa
    45661 aatggaatga aattaaaggt tctattatgt cggttgctgg tccattgatt acaggtataa
    45721 agaatgcatt tttacatgtt actttttatc tagagacctt atggaataat ttagttgaga
    45781 ttggtaaaaa cgtctttgag attttaaaaa atgttatact tgcgccggtt ttgtttatta
    45841 cctccatgat ttctggtgga tgggaagaaa caaaaaataa tatgattgct gtttggaata
    45901 atattaaaga aagtgccata aatatttggg aatctataaa gaatatattt gtaagttatt
    45961 ttacaaatat ttattttgct gcactcaata tttggacagg gttcaagctt acattgataa
    46021 atatttggaa tgaagtggta aatcaagcta aatcgatttg gttaaacata aagtattttt
    46081 ttattaatct ttggattgat ataaaatatt ttgcaattca gaaatggatt gaattgaagt
    46141 tttctattat tcaaacttgg atcgatttaa aatataatgc cattactact tggaataata
    46201 ttaaacagtt tttcaaagat acttggaaaa atattaaaga tacggcatac aatacgtgga
    46261 tttctataaa aaataccatg attaatacat ggaataacat taaggactct ttctggaata
    46321 ttgttactgg aattgttaat tctgctgaaa atgcatggac aaatctaaaa aatggtgttt
    46381 caaatgctat taatcgggta aaagaaatct ttaattcgtt aagggaagtc aatttatttg
    46441 aaattggcaa gaacatcatt gatggactta taaatggtgt aaaagaaaag tggaatgctt
    46501 tgaaagatac tatcaaaggt attgcaggta atatcaaaaa ttggattaaa ggagctttag
    46561 atattcattc tccatcaaga tggatgcgtg atatggttgg taaaaatatt gttcaaggta
    46621 ttatcgttgg tattgataaa gagcaaagta agttagatca aaccatgaca aatttagtaa
    46681 aaacaccgtc tgttcaacct gtagtaaatg gaataagtca cctaccaatt acacaaacaa
    46741 agcaaaatat atctgctagt gaaacaaaag aaattcattt acatttaaat gtttatggag
    46801 atttacctga ttcaatggtt cggaagatta ccacaaaaat taaaacagaa ttgacaagac
    46861 aaatgaaacg agatgctggt gcagtaggag gaacattata tgcaacttaa gagaggacaa
    46921 ttttttatta atcaacatta ttcttctgaa tttaatgtgt atattcaaaa caggcctgct
    46981 tctgtttcag ctagtcgagt aattgaattg agagagcgag aaggaaatga ttctatcatt
    47041 attgataaag gctattacag aaatgtgact agaaagattg agtgttacta taaagcacct
    47101 tcaattgatg tggtgcagga gtgggaagat cgaatcactg aatggttaga tatgtcgtct
    47161 tatagtgatt ttattcttta ctatgatgaa caatatattt atcaagctgt agtaatagaa
    47221 ggacctgaat ttaaaggaac aagaaaaaca ggaaatattg tcccttttga atttacagtg
    47281 agcattcgac catttaagga aaattacaat ggtagattta ctattcgaca aacagaaact
    47341 tttgaaattt ataacccaga gaagtattct tcaaaaccgc ttattaaatt gagtggttct
    47401 ggagatgctt ctttttatat taataaggac aaatatgatt tgaaatcgtt ggacagagaa
    47461 ttatttatag attctaaatt agaagaggct taccgaaaat tagatggtaa tttagaacat
    47521 caggatcaag tcactttatt tttagatttt ccatttttat atcctggaaa aaatgaaatt
    47581 aaatggacga aaaatattca ttcatttgaa ataatgccta ggtggtggag aaaagtatga
    47641 aaccaagaat atatagtcct actgaaacag atttctcaac gaatggctta ggaattttaa
    47701 aggattgtac aagatgtgaa atatatgaag tagctaatgg aaaatatgaa ttagagttgg
    47761 attatccttt aggaactaga tttgatgaat attttgaaaa tgactatcaa ataaaagcaa
    47821 agccaaacga tcaagaagag tatcatatct tttttattga tgataaagat atagatactt
    47881 ttttaaatac agtaactatt tatgctcaga gtcgtacaaa tcgacttgga agacgggtag
    47941 tcactcttgc ggaagtagac tctaaaactg gtcaagaagc gatatcaatt attgaaacca
    48001 aaatggataa aaaatctgac atacgacttt attctgatat tacggccgtt tctagcacaa
    48061 tctttgaagc gagaaatgtt ttaaattgta ttgctggtga acaaggatca ttgcttcaat
    48121 attggggtgg agaaattaaa cgtgaaccat ttaagctttc tttgttaaag cgaagaggtc
    48181 gcaataatgt tggaacgatt cggtatggaa aagatatgtc tggtttaaag gtcaaattag
    48241 attggacagg tataaaaaca agacttattc catatgctga tcctcaaagt gaggtaggta
    48301 cgactaatcg aatttatggt tcgccagtag atagtgccta tattaataac tatcctgatg
    48361 tgtatacaga gcatgttcag tttacagaag aacaaggagt aaaagacatt aagagcttaa
    48421 ataaaatagc taaaaattac tttaaaacga tcaatcctgg ctgtgataag cctaaagttt
    48481 ctattacggt tgaatttgat aagttgactg atagtgaaga agcgaaagaa tttgcgaaga
    48541 ttagaaatta tggtctgttt gatacattta aaatatatca taaaaaatat aatatttatc
    48601 ttgaatcaaa ggttagtgga ctacaatacg attctttatc tgaaaaaact ttgaaattag
    48661 aagcaggaga cattcaagtt gctttttatc agcaacaagc tgtaactatt caggataaat
    48721 taaaagatta tgcaacgaat aattatatga gtgattttaa tgattatgtt tcttcaatga
    48781 ttacaggtca aggcagtgca ggaggatatg taagtttatg gccaaaagaa aaaccttcca
    48841 acatctttat tatggataat ccagatttag aaaaggcaag agaagtacta agaatgaata
    48901 aaaatgggat tgctttttct aagaaaggat ggcgggggcc ttttacttca gcttggacat
    48961 tagatagtat atttaatgcc aattttatac aaactggatt aatcaaagca gatatctttc
    49021 aaaattcttt taacaagact ggagatgtat tgaaattagt taatggacta ctccaaattt
    49081 ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg aatggaattt tggaattcta
    49141 agggttcaat tggaacgatt ggaacaactg attctgctgg taatcctttt cctggggctt
    49201 ctactccaac ccctattgaa gataattctt tagttattcg tacaaatgga gacggcaaat
    49261 atattttgat ttctcctaaa gtaggaaaag gattagtttt attaggaaat ggtaaagcaa
    49321 tttattttgg agacttagat gtacaaggaa aacttacagt taaaggaaaa gaaataattc
    49381 ctggacaagg tggtggtcca tcgggaggtg gagaatctac tggtgggtat cctagtgaat
    49441 taaaaacaga tgcagaaaag agagcttgga gaat
//
LOCUS       DAFELO010000013        46090 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFELO010000013
VERSION     DAFELO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.013"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(23..391)
                     /locus_tag="LP452_000850"
     CDS             complement(23..391)
                     /locus_tag="LP452_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL2013600.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(1002..2393)
                     /locus_tag="LP452_000851"
     CDS             complement(1002..2393)
                     /locus_tag="LP452_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL2013601.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2606..2992)
                     /locus_tag="LP452_000852"
     CDS             complement(2606..2992)
                     /locus_tag="LP452_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL2013602.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(3048..3671)
                     /locus_tag="LP452_000853"
     CDS             complement(3048..3671)
                     /locus_tag="LP452_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL2013603.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3735..4403)
                     /locus_tag="LP452_000854"
     CDS             complement(3735..4403)
                     /locus_tag="LP452_000854"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL2013604.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4415..4855)
                     /locus_tag="LP452_000855"
     CDS             complement(4415..4855)
                     /locus_tag="LP452_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013605.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4914..5585)
                     /locus_tag="LP452_000856"
                     /pseudo
     CDS             complement(4914..5585)
                     /locus_tag="LP452_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5770..6168)
                     /locus_tag="LP452_000857"
     CDS             complement(5770..6168)
                     /locus_tag="LP452_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL2013606.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(6165..6938)
                     /locus_tag="LP452_000858"
     CDS             complement(6165..6938)
                     /locus_tag="LP452_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL2013607.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6935..7618)
                     /locus_tag="LP452_000859"
     CDS             complement(6935..7618)
                     /locus_tag="LP452_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL2013608.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7785..8672)
                     /locus_tag="LP452_000860"
     CDS             complement(7785..8672)
                     /locus_tag="LP452_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2013609.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8669..9538)
                     /locus_tag="LP452_000861"
     CDS             complement(8669..9538)
                     /locus_tag="LP452_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013610.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9675..10961)
                     /locus_tag="LP452_000862"
     CDS             complement(9675..10961)
                     /locus_tag="LP452_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2013611.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            11234..11812
                     /locus_tag="LP452_000863"
     CDS             11234..11812
                     /locus_tag="LP452_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2013612.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11812..12741
                     /locus_tag="LP452_000864"
     CDS             11812..12741
                     /locus_tag="LP452_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2013613.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12738..14474
                     /locus_tag="LP452_000865"
     CDS             12738..14474
                     /locus_tag="LP452_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2013614.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14537..15373)
                     /gene="pheA"
                     /locus_tag="LP452_000866"
     CDS             complement(14537..15373)
                     /gene="pheA"
                     /locus_tag="LP452_000866"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL2013615.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15370..15879)
                     /locus_tag="LP452_000867"
     CDS             complement(15370..15879)
                     /locus_tag="LP452_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL2013616.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15872..17167)
                     /gene="aroA"
                     /locus_tag="LP452_000868"
     CDS             complement(15872..17167)
                     /gene="aroA"
                     /locus_tag="LP452_000868"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL2013617.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRCIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(17180..18271)
                     /locus_tag="LP452_000869"
     CDS             complement(17180..18271)
                     /locus_tag="LP452_000869"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL2013618.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(18288..19454)
                     /gene="aroC"
                     /locus_tag="LP452_000870"
     CDS             complement(18288..19454)
                     /gene="aroC"
                     /locus_tag="LP452_000870"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL2013619.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19456..20520)
                     /gene="aroB"
                     /locus_tag="LP452_000871"
     CDS             complement(19456..20520)
                     /gene="aroB"
                     /locus_tag="LP452_000871"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL2013620.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20553..21575)
                     /gene="aroF"
                     /locus_tag="LP452_000872"
     CDS             complement(20553..21575)
                     /gene="aroF"
                     /locus_tag="LP452_000872"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL2013621.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(22234..23208)
                     /locus_tag="LP452_000873"
     CDS             complement(22234..23208)
                     /locus_tag="LP452_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL2013622.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(23189..25072)
                     /locus_tag="LP452_000874"
     CDS             complement(23189..25072)
                     /locus_tag="LP452_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL2013623.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(25090..27315)
                     /locus_tag="LP452_000875"
     CDS             complement(25090..27315)
                     /locus_tag="LP452_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL2013624.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27430..28464
                     /locus_tag="LP452_000876"
     CDS             27430..28464
                     /locus_tag="LP452_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2013625.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28531..29544)
                     /locus_tag="LP452_000877"
     CDS             complement(28531..29544)
                     /locus_tag="LP452_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2013626.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29622..29810)
                     /locus_tag="LP452_000878"
     CDS             complement(29622..29810)
                     /locus_tag="LP452_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL2013627.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(30250..30378)
                     /locus_tag="LP452_000879"
     CDS             complement(30250..30378)
                     /locus_tag="LP452_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL2013628.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(30381..31754)
                     /gene="rlmD"
                     /locus_tag="LP452_000880"
     CDS             complement(30381..31754)
                     /gene="rlmD"
                     /locus_tag="LP452_000880"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL2013629.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31865..32920)
                     /locus_tag="LP452_000881"
     CDS             complement(31865..32920)
                     /locus_tag="LP452_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL2013630.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32930..34360)
                     /gene="gatB"
                     /locus_tag="LP452_000882"
     CDS             complement(32930..34360)
                     /gene="gatB"
                     /locus_tag="LP452_000882"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL2013631.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(34357..35829)
                     /gene="gatA"
                     /locus_tag="LP452_000883"
     CDS             complement(34357..35829)
                     /gene="gatA"
                     /locus_tag="LP452_000883"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL2013632.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPAKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLAHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35829..36134)
                     /gene="gatC"
                     /locus_tag="LP452_000884"
     CDS             complement(35829..36134)
                     /gene="gatC"
                     /locus_tag="LP452_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL2013633.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(36240..38276)
                     /gene="ligA"
                     /locus_tag="LP452_000885"
     CDS             complement(36240..38276)
                     /gene="ligA"
                     /locus_tag="LP452_000885"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL2013634.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(38293..40527)
                     /gene="pcrA"
                     /locus_tag="LP452_000886"
     CDS             complement(38293..40527)
                     /gene="pcrA"
                     /locus_tag="LP452_000886"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL2013635.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMGFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            40682..42241
                     /locus_tag="LP452_000887"
     CDS             40682..42241
                     /locus_tag="LP452_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL2013636.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(42352..44283)
                     /locus_tag="LP452_000888"
     CDS             complement(42352..44283)
                     /locus_tag="LP452_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2013637.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(44301..45215)
                     /gene="pfkB"
                     /locus_tag="LP452_000889"
     CDS             complement(44301..45215)
                     /gene="pfkB"
                     /locus_tag="LP452_000889"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL2013638.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(45212..45964)
                     /locus_tag="LP452_000890"
     CDS             complement(45212..45964)
                     /locus_tag="LP452_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2013639.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT        12807 a        10017 c         8228 g        15038 t
ORIGIN      
        1 aaaaacaaag tctttatttc tattatttac ctgtaaccgc aaagtagttg ccatcgggat
       61 cagcaaagtt aaagacatat tcttctccaa gctgtaccat atcacctaat gtgactgatt
      121 gcgtctgcat ttttttatat agtccaaaga tatctttact gtaaaacatc aaagatgggg
      181 cactagtagc tgtgccaggt gaatgggact caacaaatgc tcgatcatat aacagaaatg
      241 aggtttgact gtcctcaact cctacttcaa ttataagcgt cccatccatt tcttgacgat
      301 ctttttccgc aaagccaact gctctccaaa acgttgcatt tttttctaca tcgtccacat
      361 aaagcatgat ttttatttca ttcgtaaaca tctgtttcct cctaaacgat ctgttctaac
      421 attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag ctctcaacag
      481 aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt ttttgttatt
      541 tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata aaaaaagagg
      601 tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca actcctcggt
      661 cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct ctttgtgact
      721 cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
      781 cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg ctgttcttat
      841 tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag aagtagcttc
      901 tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa acttcttctt
      961 atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt cacatcgatc
     1021 actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc acttgtcacg
     1081 ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc gattaatggt
     1141 tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc taaaacgatc
     1201 agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact gattgttaca
     1261 attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc tgctggtgcg
     1321 acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc aatagacaaa
     1381 ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt tgtctgagtc
     1441 tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa gctcaatacc
     1501 ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa aatcagtgag
     1561 tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt acctaaattg
     1621 acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa tactccataa
     1681 ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag actgtcgact
     1741 aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag ataagcgatt
     1801 ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact acctgctaaa
     1861 tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga taagttttca
     1921 actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc tccttgtgtg
     1981 aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa tgctgcaata
     2041 gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag cttttcagaa
     2101 actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat aactagcatt
     2161 tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga ttgagtaacg
     2221 actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat accaactcct
     2281 aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg atagaacaca
     2341 tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt catttttctc
     2401 ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga gaatgattct
     2461 tcaaccatcc tcctgaaaaa atttttttca gtgactttta tattttagac gatttttatc
     2521 gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg atagctttaa
     2581 tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa ttcaatttta
     2641 ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt acgatatcga
     2701 ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga ccttcaattt
     2761 tccgattgag gatatgattg atctgactca cacttccaga taaaacatat gtatcaaaca
     2821 ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag ttttcgatcg
     2881 tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg ttcggcaatc
     2941 tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc atataatttt
     3001 atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta ctttttaatg
     3061 atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc cgggatctca
     3121 cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt ccctgcttcg
     3181 atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg aaaagtatcg
     3241 atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc gatccctact
     3301 acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt tgattctgtc
     3361 aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc cagtacttgt
     3421 ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg gtgaaataat
     3481 tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt agaatacttt
     3541 ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt tagtgcttgc
     3601 catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa gtcccatata
     3661 taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct ctcactttta
     3721 gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc tgctaatgta
     3781 tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc aggaacatct
     3841 tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag taaatcttgc
     3901 cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg attcactgtc
     3961 atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata tctgcggtca
     4021 aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca tgtttttact
     4081 tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag atccccataa
     4141 tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc catttcttct
     4201 agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac ggcatcgaat
     4261 tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt tcctttttct
     4321 gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc gctttcgcca
     4381 tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg aattttatac
     4441 cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc tgcttttgat
     4501 gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact tgctttttgt
     4561 tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa cgttgattct
     4621 catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat ccgaaaaaga
     4681 cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa ctaaagacaa
     4741 gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt ccctgcataa
     4801 gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc atcatattat
     4861 ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa gccttaattg
     4921 gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc aatcacaatc
     4981 actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg aagaaacagc
     5041 ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat caattgagga
     5101 aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt cgttttgatc
     5161 agattccgtt ttttactgat cactttttaa aagcttttta gaatcttttg ctgacgtcta
     5221 aaaatagaaa aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct
     5281 gcagtcagat ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca
     5341 accactgctc gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc
     5401 aattctacct taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct
     5461 aatgttagcc cctctagatg gaactctggt gaaaaatgca gcaatgtttt attttcctgc
     5521 aattgaacat atagaaatgt agatcgctca atcagagtat cttttaaaac aactaaaaaa
     5581 ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct
     5641 gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata
     5701 caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca
     5761 aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat
     5821 tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat
     5881 actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat
     5941 tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat
     6001 gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt
     6061 tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc
     6121 aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc
     6181 aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat
     6241 cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg
     6301 tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata
     6361 aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag
     6421 atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa
     6481 taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca
     6541 attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc
     6601 tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag
     6661 ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt
     6721 cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt
     6781 atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata
     6841 ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg
     6901 atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca
     6961 tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat
     7021 ttttcttttg tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag
     7081 ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg
     7141 tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata
     7201 gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga
     7261 tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca
     7321 aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga
     7381 ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca
     7441 tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac
     7501 caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga
     7561 caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt
     7621 atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt
     7681 aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt
     7741 tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg
     7801 atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta
     7861 gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc acccatagca
     7921 gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa
     7981 taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc
     8041 gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct
     8101 tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca
     8161 gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc
     8221 aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat
     8281 agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat
     8341 cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa
     8401 ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc
     8461 aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc
     8521 aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac
     8581 tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt
     8641 tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga
     8701 ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa
     8761 aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat
     8821 ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg
     8881 tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa
     8941 actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat
     9001 tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa
     9061 gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca
     9121 taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga
     9181 tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc
     9241 ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca
     9301 tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg
     9361 catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg acagggggac
     9421 cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca
     9481 aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc
     9541 ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt
     9601 aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac
     9661 ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt
     9721 accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca
     9781 attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa
     9841 tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca
     9901 ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct
     9961 tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct
    10021 ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct
    10081 ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc
    10141 gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt
    10201 actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac
    10261 aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca
    10321 gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga
    10381 ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac
    10441 tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc
    10501 gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct
    10561 actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa
    10621 ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag
    10681 acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg
    10741 atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt
    10801 tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt
    10861 tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc
    10921 gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc
    10981 atccattagt tgtttacgtt tgctacattc atcataccaa aatttgaaaa cggtttcttt
    11041 attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa tcaactatga
    11101 caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt ctttaaaata
    11161 aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga ctattcttaa
    11221 ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga cgattttaga
    11281 tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag ggactttttc
    11341 cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg tggtcacaga
    11401 tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac agcaacaacc
    11461 tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta aagattcttt
    11521 taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt tttttctaaa
    11581 ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc caatcaccaa
    11641 tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa ctcgtccatt
    11701 gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga aaaacggcca
    11761 ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt gatggatagc
    11821 gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg ctatgtaatc
    11881 aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca agaagttttt
    11941 ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa tgaaatattc
    12001 caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa gaaccttgct
    12061 tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag tagttattta
    12121 acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa tttcttcacg
    12181 acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat caatcgtttc
    12241 tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact ttctttgctc
    12301 aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt ttctattctt
    12361 gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa agacaatttc
    12421 gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga aatcaattta
    12481 caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat ctttactgaa
    12541 aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa agccaagctc
    12601 tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg ctataccgaa
    12661 ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc tcaataccgt
    12721 aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt gccaaacttc
    12781 tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg tttatttatc
    12841 atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg gtactaaaaa
    12901 agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta gccgcttttg
    12961 attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat atggaacagc
    13021 ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc catccaatga
    13081 tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga aacgacatgc
    13141 tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga gtgaatgaac
    13201 gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc gataagaaag
    13261 cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt tacggatatg
    13321 ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta gtaaatcctg
    13381 agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca aatgaagaaa
    13441 atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa acaacgattt
    13501 caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca ttactcagtc
    13561 aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt attttgtata
    13621 atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat cgacacacaa
    13681 aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc ttaaaagcta
    13741 ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc aatcaattgc
    13801 taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag aaacgttcgc
    13861 ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat acgctgacta
    13921 gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga atcgacagtt
    13981 tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca gtcaaggaag
    14041 aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc gaaagaatct
    14101 cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat atgatccttc
    14161 aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa ggattcatca
    14221 cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat aacggcgatg
    14281 gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc ggcatcagcc
    14341 atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg atccaatcag
    14401 tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa aatcaatcag
    14461 aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta aataaatcaa
    14521 tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga taacagccca
    14581 atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct actaatgtca
    14641 ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta gttttcaatg
    14701 gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc agcattttat
    14761 gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt actggagctg
    14821 actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg ttcaactcat
    14881 tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt tctaatgaag
    14941 caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca gcagttgttg
    15001 attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt tgtgattgtg
    15061 ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg cctaaaagct
    15121 gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt ttggaaagaa
    15181 gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg acagccagat
    15241 cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac gcagtcaatt
    15301 gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct ggccctagat
    15361 agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct tttctggtgt
    15421 ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa gtcgttgtgc
    15481 caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa tagacgtttc
    15541 atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa gaacaatcgg
    15601 ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac cagtcgcgat
    15661 aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc gaaagctttt
    15721 ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct caatctttgt
    15781 atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga ttgttgtttt
    15841 tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct cctgacacaa
    15901 gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca gctccttcta
    15961 gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg attcgatgat
    16021 cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg atcagaccgt
    16081 ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc gcatcaattc
    16141 tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt ccttctgctt
    16201 gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg atttctccac
    16261 caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat tggttctgtt
    16321 cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct aagataccag
    16381 ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga acaagaagtc
    16441 ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc tgttgaccag
    16501 ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc cttccgccaa
    16561 attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg attacggatg
    16621 tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca ctggctacag
    16681 gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt ggtagatact
    16741 ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc atcactcgcc
    16801 ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa ggctgtccag
    16861 ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca attgattcgg
    16921 ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct tcctcttctt
    16981 cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct tctgcgcgca
    17041 aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg cagcgatggg
    17101 aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt tgttgtaata
    17161 attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta gacataatga
    17221 ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt attcctaaaa
    17281 tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct tttccgatca
    17341 gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat aaatcaaaaa
    17401 aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct tttgcccgat
    17461 tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct atttgtgtca
    17521 tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc aacaaaatat
    17581 ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag cctcctgctg
    17641 ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg actaagccag
    17701 ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt tctcttgctg
    17761 taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt tcaacatctt
    17821 ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt ttagcatctg
    17881 ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca aaccgaagat
    17941 ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc gtaatgatga
    18001 cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt gtacggacgg
    18061 gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca cttgcaacgt
    18121 aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta tcccaaccaa
    18181 ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca ccaatcagtc
    18241 caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta gtaatgtttc
    18301 aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc acttgggaat
    18361 tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac gacgctagct
    18421 gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc ttttgtatcg
    18481 atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac cccgcgaatg
    18541 acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt ggtacgtcgg
    18601 aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc tggtaatgaa
    18661 ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact aaccacagct
    18721 tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa tccagctgga
    18781 acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt ctttgtctga
    18841 tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac ttctgacgct
    18901 tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc ttggattcca
    18961 ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat ttttttggca
    19021 attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc aagcacattc
    19081 cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc tggacgcgga
    19141 cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga catgacagtt
    19201 gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc aattgttttg
    19261 ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat ccttcctcct
    19321 ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc tgacagaggt
    19381 aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga ttctcccgca
    19441 gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg tgggaacagg
    19501 aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc ttcctcttgc
    19561 tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat cgtacgtaat
    19621 cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag ttggagacaa
    19681 gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc cttctccatg
    19741 actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat gaccaaaatt
    19801 taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc gtttgatttc
    19861 caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt ctttcagctc
    19921 gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga tttcagcgat
    19981 tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa tcaatacacc
    20041 agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga ctgctgtctt
    20101 cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa cgaaatggat
    20161 cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc ctccaccaag
    20221 tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt ataactcagc
    20281 tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga ctggttcaaa
    20341 attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa cgtttgtatc
    20401 agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc cagcctgttc
    20461 tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta cagtaagcat
    20521 tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat cttttccatt
    20581 gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc attcaacgat
    20641 tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat catgccatct
    20701 gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat tcctgtacca
    20761 tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg gacagcactt
    20821 aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc gataaaaatg
    20881 acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc attgatcgta
    20941 ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc agatagcagt
    21001 ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc agccaccata
    21061 tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc atcagccgct
    21121 tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg agaagtacgt
    21181 ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat tcgcgcacat
    21241 tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac gaaactgccg
    21301 tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa ttctcgagaa
    21361 gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata gcgtgtaaat
    21421 gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg ttctgttcct
    21481 tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt ttttatttgt
    21541 tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt cttttgttta
    21601 cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt aattcctact
    21661 aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat gacccagata
    21721 atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc ttcaacgagc
    21781 cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc tacgtggctg
    21841 taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt ttgtacccac
    21901 gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca gttttgtaag
    21961 ttttctagat ttcataaaaa atcgctccaa actgatgtaa gaattgtacc tagtatagac
    22021 ataaaaaaat cgactgtcaa tcatttttat gaaacttttt ttaattttct gatagttaac
    22081 tttgtttttc agaaaaataa caattggttt tattgaagaa aatagtcatg aacaaaagta
    22141 tctgagaact tttttactct cttgttcatg acatatagaa taaagcagct ttgtcttgta
    22201 aactccgttt ttcaaataat aatctaaaga ttgctactga ataataaagc cgatttcatg
    22261 gtcctcttcg ctgctgattc ggtaaggtga ctcaacatcc gctccccagc tattgatacc
    22321 cccgactcct cttacttttg caaaaatcgt aagaaccgtt cgtctttctg gcggtagttc
    22381 ttctatatgt gttgcctgtt ccagttcttc tgctttgaat ggtaaagcgg aaaaagcaaa
    22441 tggcgtatcc tgctgttcaa atcgcaacga aaaatcttct tgatctttat ccgtattatc
    22501 caacgtttga tttcttgtta gttctaccca tttcgtttcc atatgcatgc cgcattcttg
    22561 gggaacaagg tatggggaaa cggggagtcc tgccacttta taaatacctt cgacaccacc
    22621 agccatccga tccggatatg tttcccctga taggccttca tactcaaaaa attttgcttt
    22681 cgttggcata atgaacctta aacccaatgc gggtaattcc ggtaatcctt tttgtccata
    22741 ataatgcata ttgacaagca ttttaccctc tccagcgatt tggtaggaaa ttgtcacttg
    22801 ggtttttggt actgtgttga tttccagtgt aaaaatgatt tcaaccgttt cagcgtattc
    22861 attacttgaa tattgattat ttactggagc aatcggcaaa tcgatctttt tattgtccac
    22921 cagtacttct atttctttga cttttggata caatccggct cctaaccaca taccggattt
    22981 aaatccaaat ccgctacctc tgtcattatc ggtcgttgct cgccaaaaaa ctggaagtgt
    23041 ttcccggtat agccattctt ttccgttttt attgagcgat tccagtccgc ctcttgtata
    23101 agaaaaaata taatgcgtat tccccgattt gacacctaat gtacagtcgc cgaagacgat
    23161 tgcaactttt tccgtatcca tatgactttt atttactgta tttttcataa taatatctta
    23221 cctcctgcat ggcgggtttt tcttgtccat ctgcaaaaac gatcccattc cctgagaatt
    23281 catagtcact tggtttatcg tcaaagtcac cgccatagcg catcacttct ctgcctgtga
    23341 ttggatcagt cacttggatc gcctgatctt taaaatccca tatatagcct ccttggtaca
    23401 tttcataacg atcaagcaac gtcatgtaag aatccattcc acctaacgaa ttccccatgt
    23461 catgcatata ttcacataag atgaacggtt tttccgcttt attttcaagg tagtcaatga
    23521 tctcttctgg tggcgcatac atgcagcttt ttacatctga aatcgaatct tcgaatgctg
    23581 gatttggaaa attcccttca taatggacaa gtcgatcggg gtctactttt ttgaagtaat
    23641 cgttcatttg tcgaatgttt tcttccgcat aagattcatt tccaagagac caaaaaagaa
    23701 tacttggatg gttttttaat gtttcgaaat tcgtttttgc tcgatcaaga acagccgcct
    23761 gccactgagg tactgcacct ggtacgttgt aagaaggctc aaatgcgccc attttttgcc
    23821 atgagccatg ggattcgagg ttcgtttccg ccatcacatg gattcctgct tggtcgcata
    23881 aataatacca agtgatctgg ttggggtaat gagatgtccg tacgccgttg atctttgcat
    23941 tttttatgat agccaaatct ttttccatat cttctatagt gatggcgcgt ccttttttcg
    24001 gatgccactc atgtcgattt accccgttga taatcatccg ttttccattg aagtagatga
    24061 ttttatcttt gatttcgatt ttacgaaaac cgaaggaata gtcaatatac tccgttactc
    24121 ttttttcttc gtctatcgtc tccaattcaa gcttatataa attgggctga aaatgccccc
    24181 acagtgccac attttcaaat ccatcgatgc gaatcgtcat ttgctctttt aacggatatt
    24241 gctgttgata gatttccttc ccatcaggat ctagtatcgc tacattcagc tgactgtttt
    24301 tggctacttg ctcaaaagac aaatctactt caagtttgcc attttgacat ttgtcatcta
    24361 agcgaggttt gatccaaaga tcttctaaat ggttttgtcc tcgagcaact aagaagacat
    24421 ctctgaagat cccaaaaaat cggaaaaagt cctgatcttc caagaatgca gctgtacttc
    24481 ttttgtaaac ctccacagct agaatatttt cttcttccaa ataaggtgtt aaatcaaatt
    24541 cagacggagt gaacgaatct tcggcatacc cgataaaatg accattcaac caaataaaca
    24601 ttgcttgttc tacaccagca aaatgaatcg agatttgttt tccctttaga ccttcttcta
    24661 atcgaaaagt cgtcaaatac gaaccaaccg gattgtcttt agcagctgaa aagatccctt
    24721 ccgcctcttt ctcagggctt gtcgtgaaag ggggacggcg aaatacatgt ccctcccacg
    24781 gatacatcgt attgatgtaa tggatcttgt cataatttgc taattcaata tgactaggaa
    24841 cagtgatttc atcaaaagaa caggcatcaa aatctttttt ataaaatgtt tttggtcgta
    24901 ccgaaggatt tggcgaataa caaaaacgcc atgatccatt taaagactgt ttaagggagg
    24961 atgtggtttc tacttcttcc cttgttgcat aacaaacatg atcgctatgt gcatttgatt
    25021 ggttcactct aaaaacttgt ggatcatcta accattttat atttgctttc atactttttt
    25081 ccttctctct cataactttt tgaaatgaag cagttctgtt tggaaatcac cttgaaaatc
    25141 ggggaagtaa agtccaactg tcatcaattc ggcccccgaa aatatccgct tcgaggtctg
    25201 ttcctgataa agtgcctcat catctaaccc catcagatat acttcatgga acgctggttg
    25261 agctgaagct agtatcctcc ctaaaaacac aatagcttca tcttgtttag gagacacaaa
    25321 catccatgcg gcaatatttt cttcaaaagg actcgccaaa cgataataat ggccatactg
    25381 gatcaccgga cgaatcgttt gataggatat aatttggtct tttacttgct tttcttcttc
    25441 aggagacagc tttgtcaaat ccagttcata accaaaaaca gcgctcatag ctgtttctcc
    25501 tcttgttttc aacgaagtag atctgtcggt ctgatgattt ggtacagctg atacatgagc
    25561 tgtgatggac gaaatcggat aagcaaggct tgttccatat tggattttca tacgctccac
    25621 tgcatccgtg ttatcacttg tccatgactg cggcatataa taaataaacc cggcatcaaa
    25681 ccgtccacct ccaccagaac agccttccca taagatttcc ggataatcag ttgtcaaatg
    25741 ttcaagtagt tcataaagcc cgagcatata acgatgcgct acttctcctt gtctctgtgg
    25801 gtcaagggta atcgaataga catctgaaag cgaacggttc atatcccatt tgatgtagtc
    25861 aagaggaatc gtatctaaaa tctttctcat ttgtttctca atcgtctgcc gaacatctaa
    25921 ccttgtaaaa tccagtacat gttgtgatct ggaagcagaa ggcattctac ctggctcctg
    25981 cataagaaaa tcaggatgtg ttcgatacag ttcagaatca accgagatca tttcaggttc
    26041 aaaccataaa ccaaatttca atcctttgct atgagcataa tctgctattt cacgtaatcc
    26101 atttgtcaac ttgccttcat actcgaacca gtctcctaat gaagaattgt ccgcatctct
    26161 ttttccaaac cagccatcat ctaatacgaa catttcgatg cctagctcct ttgcttcatc
    26221 aatgatcgcc ttgatttttt cactattgaa gtcaaagtaa gtggcttccc agttattgac
    26281 cagaatcggc cgttcagcaa attgatgacg acctcttgcg attctttcac gtagcaactc
    26341 atggtggatc tgactcattt tgttcaatcc ctgacttgag taagaaagga tcatttcagg
    26401 tgtttgaaaa gactggcctg cattcaattc ccacgaaaaa cgatggctgt tgatcccacc
    26461 gacgactctc aattgattga tctgatcttt ttcaagttcg aaagaatggt tgccggaata
    26521 aacaaattga agtccgatcg cttctccagt attttctgtc gtatgatgat gaactaaagc
    26581 aataaagtta ttcatatggt gactgctcgt tcctcgacgg ctttcaaata cttttgttcc
    26641 agataaaaca gattgccgtg aaatctgtct ttcattgaca tgtgctcctg gcaaagcaat
    26701 gacttcatca aatcgcctag tatgggcaaa gtctagctgg aaactagccg ctttttctag
    26761 tttgattgtt tctccggttt catttcttat ctttacagat cgtgtgatca ctgctctatc
    26821 agtaaatatg gtatagaaca aatcgaaata aatcgctgtt tctctatcct taagtgaaat
    26881 caccaacgtt tgtgcctctg acttgtccat cacataagtt tgaggcaagc cttcgatctc
    26941 tggcttccca tctgtaatat aatggctgtc atatctcaga tccagtaagt ctgatccatt
    27001 cttgcgctga atgatcgatg cgggcgttcg aaagtccatg ctgccatgag aagagtattc
    27061 ttgcggtagt gtatctttcg acagagaccg gtcaggattt aagggaacat ttcctgaaaa
    27121 tccacgatct cttcggggat aatttttatt gtctttgtac tgcttgactg gtttgccaaa
    27181 ataaatatgc gccaatacat tgctttcttc tatctttatc agataagaaa gaaatgtatt
    27241 tcgtaaatgg aagactcggc tcttttcatc aaataagatg aaatttttat ctgtttttct
    27301 atgcgtaata tccatcattt cactcccaac aaaacggaaa ccgtttactt ttttttataa
    27361 ttcctgtata ctgtttacta aacaattttg tcaataaaaa ttttttattt ttttgaggag
    27421 tgtgtaaaaa tggttggaat tagagatgtc gcacgaaagg caaatgtctc tcctgctaca
    27481 gtttcccgtg tgttgaataa cgatcagacg atccatgtgg ctcagactac tcggcaacgg
    27541 atagtagagg cagctgctgc gttggattat gacatcagta ctcgtaaata tatcaaaaag
    27601 agaatgcctt cgattggtgt gatctctacg atcggcaaag cgagcgaaga acaggatatc
    27661 tattacaaac atttgcgagt agggatggaa gaagaagctc gacgtctgca tttagggatg
    27721 aatcgaatct ataatctatc tgataatcct aaacaatggg aagatctaga tcagttaggt
    27781 gcggtgatcg ttgttgggac tgttacgaaa gaatcgatta ccgccttgtt aaaacaaaac
    27841 atgcaaatag tcgtcgtgga taaccctgac atccagcaag aggtcgatat ggtgtatgca
    27901 gatcttgaac ggatgactaa aaccgtactg gaattgttct tagaaaatgg tcataaaaga
    27961 atagcctata tcgggggata taaagtcgat atggatgaat tcggtgggaa gcagatttct
    28021 ataaatgaaa agaggatgcg tgcctatcag cattttatga tcgatcatca cttagaaaaa
    28081 tatatgtctt atcatctagg tggctggaca gaaaaacaag gtgaggtact tgcgactaat
    28141 ctattagaaa atgaatcccc tcttcctact gccattcttg tcgggagcga tccaatggca
    28201 attggtgtct atcgggcatt gaaacaaaaa aatatccaaa ttggagaaga gattgttgta
    28261 gcaagctttg ataatatcga gacaatcgca aacctctccc ctggcttgac cagtgttcaa
    28321 atcaatgcga aatcaattgg gcaagcagct gttcgaatgg cattggagcg aattgacggt
    28381 atacgggacg aaccaatcat tttgacttat cctgcacgat tgattgttag ggaaagtttt
    28441 atcccaagaa caaaaaggta ttgaaagaga ggttgtgaaa agccgcttcg tttcttcata
    28501 ctcaaaacga aacggctttt tcacaacctc ttacttatac ttattcacaa aatccaatgt
    28561 ctgctggtaa caattttgcg attcttttag cgtgtaatca aattggtatt catgggtaat
    28621 ctcttttttt gtatctgtat aaaacaagga ttgcacgggt acctttaatt ctgtcaaccg
    28681 attttctaat gcttgacctt gctcctggaa tgagtatgca tttccgtcag ttatatacgt
    28741 tggcggaaat tccttagaaa catggtcagc cacactagct tctttcaatt gctcactcgt
    28801 tttccaatct tttgttccaa tcaacgacca cgcaacggtt ttgacgaaaa acttcataaa
    28861 tcgatcatcc gaatgctgct gtgctgttag cttcaaatca acaggtccgc aataagaaat
    28921 ggttcctttc agactatctt ttggaagtag atgtttgatt ttcatttgtt tagcatacgc
    28981 atcattcgtt tgtatagctg catattgcaa tgcgatttgt gaacctgcac tatctccacc
    29041 aaaaaataat tgttccatgt ctagcaattt gtcgtctttt tcttcagcta ataaatcttt
    29101 gatcagttca ttcacttgaa gaacttgatt agggtattct gaatcaggcg ctaattcata
    29161 attcatcgca accactgcga tgttggcgtc tgaaactaat tttgtcgcaa attctttgac
    29221 acccgattta tctccgccga cgaatcctcc accatgtacc caaaatagaa caggaacagg
    29281 ccccttgctg ttttttggat agtagatgtc ataagtattc tctttatact tggactcata
    29341 cgtcaaatct gtttttttcg ccacgttttt ttctgcttga tcgaacgttt ttttatcagt
    29401 gatttgaaca gtatttgaaa acatatggtt gatcaagaat gctccaggtc tgggggaaag
    29461 ctggaaggca agaaagattg caaacgcgat cccaagaatg attccgaaga accatccgat
    29521 aaatttaaga attttctttt tcataaggct ccctcagtag ttagtattaa gcattctgat
    29581 tataccatag tgttatttcc tatcaagctc tcctagatga tttaatgatt ttccccaaac
    29641 ttcttctgca atgcttttga caaaagaaat tttttccatt gttcttcaaa caaaacattt
    29701 tcttcttctg ttgaagcaaa tccacattga ggacttaagc acaaacgatc caaaggcaga
    29761 tatcgtgtcg cttcgtagat ccggtcaatc acttgctggt gctcttccat ttcgccattt
    29821 ttcgttacag tcaaaatcta agacaactaa tttatcttca gagactttct ctaaaagaac
    29881 aaaactccca gcacgttcac tatcgtattc taaataatag gcacggacat tttcttgagt
    29941 aaaataatgg gtctgctacg tcgtcatccc ccctgatgcc gccaaaatcg aataataatt
    30001 tccgcgtcaa acatgtatat ttagtgttga atcatttgag aatatgaaaa taaaaatctt
    30061 tttgatcaat cagttaatca aattgaaata acgaaaaatc tgagacagaa tgacgttcat
    30121 tctgacccag atttgatttt gcacaactca acagtcctta ttggcaaatc gccaaaataa
    30181 tgtagactaa ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct
    30241 ttagcacgtc tatttattaa agattttagg gccttttctc atgtttttac gatcattctt
    30301 aggtgtttcc atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat
    30361 tttttctttt tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga
    30421 tgggtttgcg ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca
    30481 aataacttag catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct
    30541 ggtgacattt cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca
    30601 actacgatca cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct
    30661 tttccgactt cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct
    30721 tcaattgctt ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac
    30781 aagccaatcg tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg
    30841 gcaaactcga tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat
    30901 gactttgctg agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca
    30961 tataaaactt tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg
    31021 atgaccgaaa ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta
    31081 aagaattttt ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga
    31141 cggataatga tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg
    31201 aaccgttgca agatatctct gactactttg attgcttgat cgatgacagg atcttggata
    31261 taaaaatctt cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac
    31321 tgtccatcga tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc
    31381 attccgaccg ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc
    31441 tctacttgtt tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg
    31501 atacctatgc gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata
    31561 aatgtttcga cttttccata tccaaactta ttgcctgctt ttactactag tatttctact
    31621 tcttctccag ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc
    31681 attccttcat aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt
    31741 tgctgttctg tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta
    31801 taaatcagct aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt
    31861 ttgattaatt cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt
    31921 catccgttaa acgttcgact tcttttacga attctttgct gacaacctct aattcttctt
    31981 catctgtacc tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt
    32041 gttgatgaag attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga
    32101 tcattactgg tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat
    32161 agattattct tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca
    32221 agatctcaaa gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt
    32281 tggcatctgg cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca
    32341 tcgaagcatt tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta
    32401 ctcgagggag catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta
    32461 gttctgatgg aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga
    32521 agtaactgtt atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt
    32581 ctgctgctgc tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg
    32641 taccagctgg gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca
    32701 cttcgttgat tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga
    32761 ctctacatgc ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt
    32821 caaatcctgc ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt
    32881 ttcccgaggt ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg
    32941 ctaattcatc tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt
    33001 tcatgatttg accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga
    33061 cagattgttg gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag
    33121 ataattgcac ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca
    33181 attcacggaa gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa
    33241 tcattcctgc taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt
    33301 tcaagtaagc agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat
    33361 tttccaaaac agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt
    33421 catactctgg aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag
    33481 gcaaactagc ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag
    33541 gctctgggaa gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat
    33601 tcgttgtttc gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt
    33661 ttgcttgacg tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt
    33721 ttttcaattc tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg
    33781 catcacagcg catagagcct tcttccattt tcacatcgct gacttctgta aattggataa
    33841 ttgatcgcaa tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag
    33901 aaacgatttc gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa
    33961 taccatgcat gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt
    34021 tctttttacc ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt
    34081 gggaaatttg atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag
    34141 tatctttaga gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag
    34201 ctttattcat tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt
    34261 ttggttctgc accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact
    34321 cgacgtggac ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat
    34381 agctggttgt tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat
    34441 cgttttttca tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa
    34501 acctgccggt acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc
    34561 actcatgtac attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg
    34621 tgaagatggc ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat
    34681 caatgtacgg acttgtcctg cttttttgaa gtaagcgtca taataacccg cgcttaagga
    34741 gaatgtacct aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt
    34801 gacatataca tcatctaatg atttcacatt ttcagagcga taaccgtagc ggataccatc
    34861 gaatcgttgc aaattggaac ttgcttctga tgaggcaatg atataataaa cagccacacc
    34921 gtattttgaa tgagccaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc
    34981 tgctgctttc aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt
    35041 aggtaaagca atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac
    35101 tgaaacgcct gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa
    35161 tgcattatct ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc
    35221 aatcaaacca aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa
    35281 agctgctggt tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc
    35341 agctgcaact gctgctgcag aaccaccaga agagccacca ggtacttttg ctgggtccca
    35401 agcatttttt gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc
    35461 catgtttagc ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc
    35521 atcataaatt ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt
    35581 tgttacgata ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat
    35641 ccctttttca tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa
    35701 tgcctcgacc tcaggctctg tttctttgat tcgttcaaat gttgtttttg tcaaatcttg
    35761 tacagtgatt tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa
    35821 ttctgtcatt atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg
    35881 tcttctgttt ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga
    35941 tcttgtcgca tcacgtttac tgtttcaacc acattcgacg taaatggaac accttccgta
    36001 tctacttctt ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt
    36061 gcaagttctt catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct
    36121 tcactaatcg ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt
    36181 aacacaagag tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt
    36241 cattcttcta ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact
    36301 tcgattccta attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg
    36361 atatccgttt ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct
    36421 tttgcttctt cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta
    36481 aaaggagact cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg
    36541 cctgcttgtt ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact
    36601 acactgtcgg caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt
    36661 ttactcaatg tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg
    36721 acatgtcgga ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg
    36781 attgctgtca aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct
    36841 tcttctgtca atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt
    36901 tctagtacgc gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc
    36961 aaaccttctt tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca
    37021 tccaaatgga caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg
    37081 ctgtctttag gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct
    37141 ttataaatca gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta
    37201 tgcaggctgg cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt
    37261 ggcgttacta cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact
    37321 tcttctggcg gaaacttgta agctgctgcc catctcggtg ctttgatagt aaaaccaagt
    37381 tgatcttgta gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa
    37441 tccacacgtt tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat
    37501 agacgttttt cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac
    37561 tgggtttttg cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt
    37621 cttttcgctg tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga
    37681 ttcgcaaaga tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt
    37741 tttggcatga aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg
    37801 accgaccgga cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga
    37861 gtcgcacctc tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt
    37921 tttagttcac agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa
    37981 gctatcagct cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg
    38041 actttttcaa aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg
    38101 atcaaatcag gatattgttc ctcgattgct actaattctg cgtattcttt atcataaaca
    38161 tagtcttcca ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt
    38221 gtacgaagtt cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt
    38281 taccctccac tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc
    38341 ttgacttggg aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac
    38401 tgttcctaga ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc
    38461 ccctccagta gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc
    38521 tgttttttta gcgaatacag gcgttttttt aggcgtagga cgcatgcctt cgatttccag
    38581 taactcctga tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata
    38641 taatgttctg gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac
    38701 atatgcgaga cggcgttctt cctccagttc gctttcttcc atcaatgctc gagataaagg
    38761 aaaaaccccc tcttctagac caatcaggaa tacaactgga aattctaatc ctttggctgc
    38821 atgcaaagtc atcaatgtca cttgggaagc atcttcttct aaattgtcaa tgtcggaaac
    38881 aagtgccaga tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc
    38941 attttgctgt tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt
    39001 ttcgatgcgt gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct
    39061 gcgatccaaa acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac
    39121 ttcttggatc atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc
    39181 cagatccaca ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg
    39241 tagtttttcg atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa
    39301 actgattgaa tcttgaggat ttaccagcac attcaaatac gctaaaatat ctttgatttc
    39361 tttacgatcg tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag
    39421 agtttcttcc attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta
    39481 attccgatga ttgcttcgaa tctcttcttg catccggtct acgataaaac gggtttcgtc
    39541 tcgttcatta tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa
    39601 gttttttggt tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt
    39661 tttcgtggag cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc
    39721 aaaatccaag atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc
    39781 tcccacgacg cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc
    39841 gtgatttgta tcttggtact catctacatg aatataatgg aatttgtttt gataatacgt
    39901 gagtgaatca ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagcc
    39961 catacattga ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc
    40021 ttcgtaataa ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc
    40081 ttgagagatt gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc
    40141 ttttagtatc cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg
    40201 gttatacccg ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt
    40261 cgatacccag acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc
    40321 ttttgcagcc ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc
    40381 ctcaatcaaa taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc
    40441 cattagcagt aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc
    40501 gtttaataat tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa
    40561 ttatagcatt ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta
    40621 tggatttttc cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg
    40681 aatggaaaaa aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa
    40741 aggaatattt gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga
    40801 aaagctaagc gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt
    40861 aattggccta gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa
    40921 tgaaccggat atcaagggaa gcgggattcc acaagtagtg ggacagcttc gcggggaatt
    40981 atcaatgaac tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg
    41041 tgcgggcctt tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca
    41101 aggagctgga caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg
    41161 tgcaagtagc ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt
    41221 agaagaaatc catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat
    41281 cactgctaac ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga
    41341 ccttccttct ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg
    41401 catcttaggt tttatttatc agaatgtctt gctggctttg ccaaaatttt acaaccgatt
    41461 gcctttacca gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta
    41521 tttatggcct agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc
    41581 cttgccaatc attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc
    41641 ttacggtggg ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat
    41701 cggtagcttt tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga
    41761 ttttgtcgtt attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc
    41821 aattatttta gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc
    41881 tgctcttgtt gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt
    41941 attggaacgt ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt
    42001 tgattttcca gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg
    42061 gcctaaaaat atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg
    42121 tgatacgatc atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc
    42181 aagaatcaag caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta
    42241 acgaacatgg aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga
    42301 ttctataaaa aaagaagagg ctgggaaaac ccagcctctt cttttttatt tctatgcttc
    42361 tgctactgca ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat
    42421 agcaataaac accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc
    42481 gtgaggagcc agtaatttga ttccgaaagc tccgactaat ccgccagtta aagcagaacc
    42541 aataataaaa cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc
    42601 cgtgacaaac gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct
    42661 gaatttattc ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc
    42721 agccataaca gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc
    42781 tgtaccaaac acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc
    42841 agctaataat gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt
    42901 caatgcatca ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat
    42961 cagtaagcca aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa
    43021 gttcttgaat aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag
    43081 tgcacctaag aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat
    43141 caaaccaggg cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa
    43201 accaaatgca gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag
    43261 atttgataat tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat
    43321 agcgattcct ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt
    43381 gtaaattctt tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc
    43441 atcaccatgg aatactggtg cagtaccact aagggctaga ttgatcaact cttctggttt
    43501 acggatacca tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc
    43561 tacttttttg tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa
    43621 tcggtttttg attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt
    43681 cgcttttttc ttcaaagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc
    43741 tgtaacggca acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc
    43801 ttgttctgct ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac
    43861 ttcttctggt gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg
    43921 agatagtgct gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat
    43981 gaaaaacaga taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt
    44041 tgcgaataaa actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc
    44101 atcaccaaga cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc
    44161 gatatcggaa atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt
    44221 tttatcagtc gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt
    44281 catgattatc ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca
    44341 tcaatcaatt cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact
    44401 ccccatttaa aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccagca
    44461 atcattgaat cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta
    44521 taagttcctt gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc
    44581 tgtgcccctt cagctagtaa ttttttccca tacggaatga tttcttctac agattggaag
    44641 gttgtgttgt aaagctcagc taattcgtga ttgtttggtt tgatcaacaa cggtttctta
    44701 acaagtgcat tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct
    44761 cgaatgatat caatcagttc ttgataaaat ccttttcgca ggctagctgg cgcgcttcct
    44821 gacaatacga cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt
    44881 tcttcatctg aaatagatgg gcctaagcca ttgatctcag tttcagtgtc tgattttagt
    44941 ttgatattga ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt
    45001 tcaagccact tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat
    45061 tgagaatcga tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg
    45121 aagtcttcct tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa
    45181 tcaattgatg gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct
    45241 tttcacggaa ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt
    45301 ccaaaggtgc aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa
    45361 atgtctcttc ggaatttttc atggctaaac gtttaacagc tgcctcttct ggatccggag
    45421 tcgtgaatcc ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa
    45481 aattttttag ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg
    45541 ttccacctaa aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg
    45601 aatttgtcac tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag
    45661 agccagcgtc taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga
    45721 tttgctgttt ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca
    45781 ataattgctc tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct
    45841 cttgtaaatc acgtcttatc gttgattcag aagcattcaa aaggtttaca agatcttgtg
    45901 atttcacgat tttctgctgt tcaagcaagt ctaggatttt ttgtctgcgt tcctctgtaa
    45961 gcattttcac gatcacctca atacaaataa tagcatattt tttttctact agcaatataa
    46021 atcagtcaaa aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca
    46081 gaaagactgg
//
LOCUS       DAFELO010000014        45187 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFELO010000014
VERSION     DAFELO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.014"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(177..491)
                     /gene="trxA"
                     /locus_tag="LP452_000891"
     CDS             complement(177..491)
                     /gene="trxA"
                     /locus_tag="LP452_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL2013640.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(578..2938)
                     /locus_tag="LP452_000892"
     CDS             complement(578..2938)
                     /locus_tag="LP452_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL2013641.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(3053..3601)
                     /locus_tag="LP452_000893"
     CDS             complement(3053..3601)
                     /locus_tag="LP452_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL2013642.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(3659..4096)
                     /gene="zapA"
                     /locus_tag="LP452_000894"
     CDS             complement(3659..4096)
                     /gene="zapA"
                     /locus_tag="LP452_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL2013643.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4298..5215
                     /locus_tag="LP452_000895"
     CDS             4298..5215
                     /locus_tag="LP452_000895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL2013644.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(5471..6382)
                     /locus_tag="LP452_000896"
     CDS             complement(5471..6382)
                     /locus_tag="LP452_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2013645.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(6785..7363)
                     /locus_tag="LP452_000897"
     CDS             complement(6785..7363)
                     /locus_tag="LP452_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2013646.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(7383..8051)
                     /locus_tag="LP452_000898"
     CDS             complement(7383..8051)
                     /locus_tag="LP452_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013647.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(8066..9136)
                     /locus_tag="LP452_000899"
     CDS             complement(8066..9136)
                     /locus_tag="LP452_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013648.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(9307..10671)
                     /locus_tag="LP452_000900"
     CDS             complement(9307..10671)
                     /locus_tag="LP452_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2013649.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(10668..12227)
                     /locus_tag="LP452_000901"
     CDS             complement(10668..12227)
                     /locus_tag="LP452_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013650.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(12283..14763)
                     /locus_tag="LP452_000902"
     CDS             complement(12283..14763)
                     /locus_tag="LP452_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013651.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(14747..15082)
                     /locus_tag="LP452_000903"
     CDS             complement(14747..15082)
                     /locus_tag="LP452_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013652.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(15096..16130)
                     /locus_tag="LP452_000904"
     CDS             complement(15096..16130)
                     /locus_tag="LP452_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL2013653.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(16220..16900)
                     /locus_tag="LP452_000905"
     CDS             complement(16220..16900)
                     /locus_tag="LP452_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2013654.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(17184..18287)
                     /locus_tag="LP452_000906"
     CDS             complement(17184..18287)
                     /locus_tag="LP452_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL2013655.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(18916..19386)
                     /locus_tag="LP452_000907"
     CDS             complement(18916..19386)
                     /locus_tag="LP452_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL2013656.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            19716..21356
                     /locus_tag="LP452_000908"
     CDS             19716..21356
                     /locus_tag="LP452_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL2013657.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            21510..22832
                     /locus_tag="LP452_000909"
     CDS             21510..22832
                     /locus_tag="LP452_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL2013658.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(22900..23331)
                     /locus_tag="LP452_000910"
     CDS             complement(22900..23331)
                     /locus_tag="LP452_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2013659.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(23396..24232)
                     /locus_tag="LP452_000911"
     CDS             complement(23396..24232)
                     /locus_tag="LP452_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL2013660.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(24801..25106)
                     /locus_tag="LP452_000912"
     CDS             complement(24801..25106)
                     /locus_tag="LP452_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL2013661.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(25131..25562)
                     /gene="ruvX"
                     /locus_tag="LP452_000913"
     CDS             complement(25131..25562)
                     /gene="ruvX"
                     /locus_tag="LP452_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL2013662.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(25559..25825)
                     /locus_tag="LP452_000914"
     CDS             complement(25559..25825)
                     /locus_tag="LP452_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL2013663.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(26080..26805)
                     /locus_tag="LP452_000915"
     CDS             complement(26080..26805)
                     /locus_tag="LP452_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL2013664.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(27166..27236)
                     /locus_tag="LP452_000916"
     tRNA            complement(27166..27236)
                     /locus_tag="LP452_000916"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27202..27204),aa:Gly,seq:ccc)
     gene            27353..28105
                     /locus_tag="LP452_000917"
     CDS             27353..28105
                     /locus_tag="LP452_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL2013665.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            28121..28600
                     /locus_tag="LP452_000918"
     CDS             28121..28600
                     /locus_tag="LP452_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL2013666.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(28851..30431)
                     /locus_tag="LP452_000919"
     CDS             complement(28851..30431)
                     /locus_tag="LP452_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL2013667.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(30424..31167)
                     /locus_tag="LP452_000920"
     CDS             complement(30424..31167)
                     /locus_tag="LP452_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013668.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(31343..32119)
                     /locus_tag="LP452_000921"
     CDS             complement(31343..32119)
                     /locus_tag="LP452_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2013669.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(32367..33203)
                     /locus_tag="LP452_000922"
     CDS             complement(32367..33203)
                     /locus_tag="LP452_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL2013670.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(33214..34143)
                     /locus_tag="LP452_000923"
     CDS             complement(33214..34143)
                     /locus_tag="LP452_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL2013671.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            34335..34688
                     /locus_tag="LP452_000924"
     CDS             34335..34688
                     /locus_tag="LP452_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL2013672.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(34762..35418)
                     /locus_tag="LP452_000925"
     CDS             complement(34762..35418)
                     /locus_tag="LP452_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL2013673.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            35644..37578
                     /locus_tag="LP452_000926"
     CDS             35644..37578
                     /locus_tag="LP452_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2013674.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(37664..38887)
                     /locus_tag="LP452_000927"
     CDS             complement(37664..38887)
                     /locus_tag="LP452_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2013675.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(38881..40779)
                     /locus_tag="LP452_000928"
     CDS             complement(38881..40779)
                     /locus_tag="LP452_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL2013676.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(40773..41891)
                     /locus_tag="LP452_000929"
     CDS             complement(40773..41891)
                     /locus_tag="LP452_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2013677.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(41974..42807)
                     /locus_tag="LP452_000930"
     CDS             complement(41974..42807)
                     /locus_tag="LP452_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2013678.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(42800..43717)
                     /locus_tag="LP452_000931"
     CDS             complement(42800..43717)
                     /locus_tag="LP452_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013679.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(43944..45068)
                     /locus_tag="LP452_000932"
     CDS             complement(43944..45068)
                     /locus_tag="LP452_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2013680.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        13475 a         9171 c         7734 g        14807 t
ORIGIN      
        1 ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa ggtctgatct
       61 ttgaaaacta aaaaatatgc ggcgcacaga acgcgccgca tatcggacta agttttattc
      121 aaatttgtct tcaccaacac gatttgacga agagccctta tttttgctgt aagtttttat
      181 aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac agctttttct
      241 actacttgac catctttttt caacattaat gttggaatgc tcatgatacc gaattgttga
      301 ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc ttcatcatat
      361 tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca ccaagttgcc
      421 caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt atctgtaatt
      481 acttgtgaca tatcaaagac ctccattttt aaaatctatg attagtatag catttctata
      541 cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta acaattgtcg
      601 caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc cgatggtttt
      661 taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg acaatagtca
      721 cttgaggata accagctaga atggcagaat ccaaatattg atctacctca cttagcgctt
      781 cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta gattcagatc
      841 gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc gtcagctcgt
      901 cttcggacac attcattttt aatataccga tttctacttg ccactgattc ccgtttttac
      961 gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc ggtttcaacg
     1021 ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct tcttgatgta
     1081 gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt ccttgcccaa
     1141 tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct tccgcctctg
     1201 acacgacttc gtttgctttt ttctttgctt tttccatttc tttttcacgt tcttcaaaga
     1261 aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa acaaaatgac
     1321 gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct gtgatcattt
     1381 cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata acttctgtat
     1441 caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg ccaatcagca
     1501 aacgatacgt tggacttagt gtatccacat cgaactccat gctggcatta atcgtatttg
     1561 cccgattgta tccataaact ttcaattcag gataatgcgt tgttgccatc acataagcag
     1621 actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct tgcggatctg
     1681 ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt gataaaatat
     1741 ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc gactgttcgt
     1801 ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca cctgcaggaa
     1861 tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac gtgatcgttt
     1921 tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg attgtgatgt
     1981 cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc aagacgacat
     2041 gattgtcttc actgattccc ggcacgatgg cttttacttc tttaccaaat cgtgcttttg
     2101 cattcatcag atccatttta ccaatcacat aagcattgtg gatgatttct tgacgatggg
     2161 gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt tcagcgattt
     2221 ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata aataaagtct
     2281 gtcctgaagc actttgatcg tggactacac cgccaaatac accgcggtat tcttgtttga
     2341 ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa tactttgcat
     2401 ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt cgccgaatgt
     2461 tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg tcttcatcga
     2521 ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct gcccacatat
     2581 acaaacgtgc aaattcgatt tcactgtcag aaagatcatc aatgaaacga ttcaattcgc
     2641 ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag tctgctccga
     2701 tctcgatccg tttcatatgg ggacggatat tttcaagttt aggtataggg atcccgcctc
     2761 gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg atcgtttgtt
     2821 tctcactcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc actacaaatt
     2881 gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg ttattcattt
     2941 tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta cgcagccttc
     3001 ttaaataaac tactctgtac aaaaaacgaa atattttcca gcactatttt ccttaattca
     3061 aaatattcgt tacccaaagc tcatacatct tgttggtaag aaatggcgtt ctctcaacga
     3121 taaaacgagc gaaactatgc tcgccgaatt gattttggac cacatctaat ggaacaaagg
     3181 caagtaactg caaaatcaaa aatatagtaa cgtaagccat gattagtgac aggatgcctc
     3241 ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt caatccatgg acaaaaaccc
     3301 ccacaaatcg ggtaagcagc cagccaatta gcaaaataat cagaaaagca gttcctgcat
     3361 aaaaagcttc atctaggtga aaagcaaata cctgatcaaa aaatgctaac tttgatgttg
     3421 gggtgacagc tgggtatgga atgtataatt ccaaatgact tgctaatggt ttgtaaaaat
     3481 gctgggcagc aataaatgac aatacatatc caatcgcgaa tatgatttgc aaagcaaacc
     3541 cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa taaaatcagc aaactcagca
     3601 tgctacgttc ctttctcttc taactttttt aaatttttct tattttgata aaaggatctt
     3661 aattagaaga tttttgttta atttgtcctt ttcgtttctc gtttaaaatc tgttgtgctt
     3721 ccacatgatt tttaatggta tgatctgctt gaccattttc tttcaatact tcccgagctt
     3781 cgttctcgat tgcttcaatg cgtttcacac gattttcaag ctctgtaaat ttgatcattt
     3841 ttttctttaa ttctgctgtt tcttcttcca gctccaagat tctttcttgt ttcttcaatt
     3901 gatccgaaag cgcattgaca gccatcaaga ttgctgcttg ttcattatcc atttgtggtg
     3961 ataattgttt caattctgct aactgttcat tgatcagctt cgttacgata tccatatgat
     4021 gcttggtttc ccgtccgata atagtatatg tttggttggc aatcactgct ttgtagcgtg
     4081 ttttttcgtg cgccatacga atcatttcct cctatttgat gaaagtttta tttagtgtac
     4141 catgaagttg gttctttcac cgttctccac aaatattttt cacatactta actattatat
     4201 gataaacttt aaaaaaatgc tatgtctcgc aatctcttag aaaacgaaaa gtcgtgagtt
     4261 gcggtatagt tttatgtata aaaacgagga gaattttatg agcaacgtca ttatcaaagc
     4321 aacaaacgac caacttagtc aaatgaaagc atactatcgc tcttatttgt taaataaaca
     4381 gattccttat actagctttg ctgcaaaaaa agacgggacg accattaccg cttatacatc
     4441 cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa gctgataaat ggggggaggc
     4501 acaggataaa caagccaagc cagcaaaaac agcctcttct ttgccaaaaa atatcagtca
     4561 attatccgtt ttaggtagtg atgaggtcgg aaatggcagc tattttggtc cgctaacagt
     4621 atgtgctgct tatgtgaaac gtgatcagct tgcagaatta cgtaaacttg gtgttagaga
     4681 ttcaaaagag ttaaaagata gccaaatcat ccagcttgct gacatcttaa aaaaaaccat
     4741 cccctataaa ctgcttgtct tatctccaga aaaatacaat gagatccaac caaaatacaa
     4801 tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc catttgctgc taaaaaaaat
     4861 cgagccagaa aaaccagaag cgattttgat cgatcagttt acgccggaag caaattttaa
     4921 aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa ctgtacttcg ttactaaagg
     4981 agaacaatat catttagctg ttgctgctgc ttctatcatc agtcgtgcca gctttttgga
     5041 agagttacac aaagcatcag ctgaagctgg aatcaccctt ccatcaggag ctggaacgaa
     5101 atctgaccaa gtagcggcaa aactattgga gaaaggcggc atgcctatgc ttgaaaaata
     5161 tgccaaactt cattttgcca atacagaaaa agctttgaag ctgctaaaaa aataatggaa
     5221 ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa acggtgttct catgccaact
     5281 cctcggtcac aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgctcct
     5341 tgtgacgcgg agttaaacgg caggctcaca acctcttcct taaacggtgt tcaaaagctt
     5401 gcatctgcga taataagccc ggccaaacga aaaatatgag gagctatttt tgtttggctg
     5461 ggcttattta ttactgaaaa taagtggtca atgcttgtgt caaacttgca gcaatctgct
     5521 gctgataact gtctgttact aattccttta aatcactatc attattcata taacccatct
     5581 cgattaaaat agaaggttga agattttctc gaagaacttg atagttccca gtggagattc
     5641 ctcttgaatc cagaggcaga tcagtgaagg agtcagcaat tgtctgtgct aatgtttcct
     5701 cttgtccata gtaataatat gtcgtgattc cagttgcttc attcgcatat tccgtagagt
     5761 ctgcgtgtaa actgataaag acatctgcct ttttttcttc actgtagtcg catatatctc
     5821 caagctgtac taattcatct gtagtatggg tcaaataaac cgttgctcca gcatcctcta
     5881 gcgccttttt tacttttatg gctgtactta atgttatatc tttttcttct gtttgttcat
     5941 cattgcttaa tgccccagta tcatttccgc catgcccagg atctaataca atcactgcat
     6001 cagatagctc ttccggtttt gtttgtatat tctttgtgac tttggttcca tcttcgaggg
     6061 ctaaatatcc agtatagcca tcatttgtct tgatctgata agcatttttt gtttcagaaa
     6121 gaaactccgc cagctctccg ccgtttatag ttgcaagtgg ggtagttagc gagtcatctg
     6181 tatatagtgt acggtcattt tttccgattt tgattaatgt ctcttgttct gtatcatcaa
     6241 atgattcctt tgtctttgta accgtactgt tcgtactgct gaccgtttgt ccaactatct
     6301 ttttttgacc aaaaacatct atttgaaaac agataataat gataatgatt gcaaatacaa
     6361 aagctccaat tatttttttc attcttgact ctcacctcgt agatccagac taagggtcaa
     6421 aacttaaaat cagcttaagt acgcaaaaaa ggggctgtaa aagatataac cgtagaaaat
     6481 aacattactg ctaaaatgat ttttttcatt tttggcatat cctgaaatct ccttttgact
     6541 attcgtggtg attcatctac ccaataaata gtttccttca ttctacaaac aattaatttc
     6601 ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa atataccctg
     6661 ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata aaaaaaacaa
     6721 gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc cctatataac
     6781 agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc gccacattta
     6841 gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact aagaaaaaat
     6901 gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt gctgaaaaga
     6961 tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga tctcttgttt
     7021 cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc agatgtgtct
     7081 caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg cttgtttgaa
     7141 cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact gccgtatata
     7201 gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca actttttgtg
     7261 cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca gtcgcttctt
     7321 tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca cctgcttttt
     7381 ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc gcatttgtca
     7441 atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt tgattctttt
     7501 gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt tggttcatct
     7561 gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct ttgccgttct
     7621 ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag ctgctcaaat
     7681 aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa taataactgc
     7741 ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc aatttcttta
     7801 aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc attgataaat
     7861 acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt acttttacct
     7921 gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat ggttgtttct
     7981 ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc tttaaattct
     8041 attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg atttttacga
     8101 ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac aagctaatga
     8161 cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca agggatgttc
     8221 ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat gtttgtgcca
     8281 ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca ccaaagatat
     8341 taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc gcaataacga
     8401 tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca ggtaattcat
     8461 tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg attttacctc
     8521 tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg aagcggatct
     8581 cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg gcatgatccg
     8641 taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta tctcctacgg
     8701 ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg ttatcaaaag
     8761 cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg aagaagctaa
     8821 cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt gtctgggcaa
     8881 gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc atggacatcc
     8941 ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac ttgtcgactg
     9001 ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat accaggtagg
     9061 cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc ataatttcat
     9121 ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca acaattttat
     9181 cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat ttcattatat
     9241 gaatttttta ttctcgattg attaatattt attatatccc cacacaataa aagaccgtga
     9301 tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta gtactttaaa
     9361 agctaactca ttgaggtagt gaaaacgaga ggtgtgttca gacagaaatc caaaatcctc
     9421 aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca ggttcaaatc
     9481 tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg tatccggatg
     9541 gtagatcagc tttgcattta ctgttttgtt ttcttcatcg tattgtggaa atagatcaaa
     9601 ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt ctgtcagccc
     9661 tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc tgaggtcgta
     9721 agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta cagctcgagt
     9781 aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga cgatatctcc
     9841 aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca cagtctcatc
     9901 ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa
     9961 aatgaccata tctgccttta ttgcttgaga tagaagttgt acggatacta ctttctcttc
    10021 tgattttttc caatcgatca agaattcatt tggataaaaa ttgatttgct tagtttgcag
    10081 ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa cattcggcat
    10141 ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta attccaatcc
    10201 aatataacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg caagtacttg
    10261 cttagattgc actagatttt tataggtgta gacattttca gcttcaattt gtttatacaa
    10321 ggtgacagga gaggaacctg ttgctacgat taatttatca taggttatgg tctcgttttt
    10381 tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag ttactctcca
    10441 ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg caacttcagc
    10501 taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat agcctagtcc
    10561 atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt
    10621 tagggcagct gtgatgcctg catgggaagc accaatgatc acgattttca tgttttattc
    10681 atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa ataggggaaa
    10741 tcataattat attcgttgct agtgaataca aaaactttag cggacttctg tgacgggtac
    10801 tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta ttcgactaaa
    10861 tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat atccgccatt
    10921 agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg cttgatcagc
    10981 gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc ctcgtctact
    11041 tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa aaccaggaat
    11101 tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa aatattcatc
    11161 catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc
    11221 gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg tttaaacgtt
    11281 cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca
    11341 tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt
    11401 tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa tgtatggagg
    11461 tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata atctggtttg
    11521 gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc aaatttcaat
    11581 cgtccttctt ctgtaatagt aaaactaaac tctttttgca gcagatctaa aaaagtgttt
    11641 cctgataaat cactgtattc ttttttgttt tcaagataag gacgtccaaa ccaaaataat
    11701 tggaaataaa aataacaaat ttgactttct ggaccggtca gccgcccgct ttttattttc
    11761 agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa agtagccgaa
    11821 ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc caacagataa
    11881 agaagtatct gatattttgt agaatagcgc aagtagcaat gataataaac atcgactgct
    11941 gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat ttccaattgt
    12001 ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt agcccgatca
    12061 actgataagc gggtaagtac gttattaacg gaaatctctc ctttttcctc taataaaagt
    12121 tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag ttccaagtcc
    12181 actcctccaa gcatttctga ttttaacagc agaagtttcc ttctcatcct tgaaaaagag
    12241 gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg ttggcccagc
    12301 tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat tcgtactgct
    12361 atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct ctcctttagc
    12421 tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt gttttgatcc
    12481 agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat tcttaaataa
    12541 agaagtcaat gtccctgtca gttgccaagg agacgtgctt ggaagccgag tatcccgcac
    12601 atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc ttcaacggtt tggggagatc
    12661 gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt tggggacaag
    12721 aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat ctgctaatgt
    12781 aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta cagaagtatc
    12841 tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt tgctcaatag
    12901 atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg taccatttaa
    12961 ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga tttttttgtc
    13021 ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc
    13081 tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt ttagctgact
    13141 atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg tgattccagc
    13201 ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta catcgtccac
    13261 caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag aagtagctgt
    13321 atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc
    13381 taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt ttagttcagt
    13441 tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc cccactgact
    13501 atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt
    13561 taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca cgatattggc
    13621 tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc cttgtgtaac
    13681 gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca actgatcaaa
    13741 acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg gaaggttctg
    13801 gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc ctgttgaatc
    13861 acttgttgag atgtaactat tccacgtatt tatctctgca cttgcagaaa atgcatatgc
    13921 tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag ctggagctaa
    13981 aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat caacagaaaa
    14041 aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt tgtttggact
    14101 cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa catcaaattg
    14161 aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa tatctgccaa
    14221 tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc ctgctgataa
    14281 taaactaccg ctagtaacct tgatgggggc accggcaata tacaagtagg cgttgttcca
    14341 gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga tgatcgccac
    14401 aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta gtttcacatg
    14461 gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac catcgttcaa
    14521 ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa gcttcccgcc
    14581 attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt atggatcata
    14641 agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga taaatatact
    14701 taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa
    14761 catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga agccataatt
    14821 ctataccaat gatcaaataa agaaaacgga tcttttcttc attcgtctcc gggaatacgc
    14881 catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat gcgccattga
    14941 ctgaaccact tggatgcgga actggactta gatcataaaa tgtaatcgta cctgaacttt
    15001 tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca ataatccctc
    15061 ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc ttttttcttt
    15121 ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc agcaaccaat
    15181 cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt ttcatttact
    15241 ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc attactgtcg
    15301 cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc accagctaaa
    15361 tcaagattca agtgaaaatt cgaatttggt gcaactaaaa attcattaga cgtattttca
    15421 atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct gataatccta
    15481 ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc gtccaaatct
    15541 cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa aacgactgcg
    15601 agattataag caatacgatt tttgatttgt gctccttctg tagcattttc tgtttcagct
    15661 tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct ttttgctggg
    15721 attttcactt ctataggtat agttgtttct gcatgtgctg atacattgat ttcctttttc
    15781 gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt tgggtttttt
    15841 tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga gacctcaaat
    15901 gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt ttgttccggt
    15961 gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg aaataccggt
    16021 ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa aatgaaaaat
    16081 agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat tgtccaccac
    16141 atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc accgcagttt
    16201 gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa gtcaaatcag
    16261 ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct ggatcataaa
    16321 cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt gtcgctgcac
    16381 ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg ttatctgacg
    16441 tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt
    16501 ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca tctgcaccta
    16561 cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg tctacttgat
    16621 acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcagga actacatcca
    16681 atgttaaaga acctgtctgg ccagttgctg gattttccgg atctgctggc tctactggtt
    16741 ttgaaggatc agtagggtta agaggctcaa cggtatttgt tggttcagta aaggtaatat
    16801 ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg cctagaagaa
    16861 gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac tcctttttct
    16921 tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc atccgcttac
    16981 ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat
    17041 attcttcctt ttttattaag tatgaaaaaa tatttcaatt ttattttttc gtttcaaatt
    17101 attctattag tgacatcctt gaactataaa aataggagta cggcatcatc tgccgcactc
    17161 ctattttttt cgtcatttat ttcttaaata aaacgattct cacgcaattt tatttttttg
    17221 atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc tgttttaaag
    17281 aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata aataacgggt
    17341 ctgccaatag caacaagatc ggcacctgat gcgattgctt tgaaaacatg ttgtccacgt
    17401 cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc cacatattgc
    17461 aacgaatcaa acgcagcagg ccccccatct aattgacggc caccatggtt agatacccaa
    17521 attccaccag caccagattc taatgatcga tagacatctt cttctgattg aacacctttc
    17581 acgtagactg gcaaatcaga atgagcagcg ataaattcta catcttttgg actaagtttt
    17641 tgtttggaag atttatatac agcatccatc gtttggccta ctcctgattg ataagcctga
    17701 acgattggca ttggcagcgg gaaggtaaac ccgtttctgc ggtctgtttc acggtttccg
    17761 ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt ggccacttca
    17821 agaatatcca aattgattcc attatctttg ctcatataaa attgaaacca ttgcggtgct
    17881 ttctccccgc ctgcttctct tatctcttct aaagtacaag aagcgtaaga acttgctgta
    17941 tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt cacatgagct
    18001 aatccatgag cagcaactgg tgccatgatg attggtgcag tcagcatttc ttcgtcaaaa
    18061 tgtgtagttg tatcgggtaa ttctacatct cttaatacat gaggaataat caaacgatga
    18121 ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact gctgatgtaa
    18181 ccataaccac cagctggaat cacttgtgaa gccgcgattt ctaaatcttc catattgata
    18241 aaatctacta ttccttcatt tgtacctgct tgatatgttt tttccatctc aatgccttct
    18301 ctctataaaa tttgaatgat gaacaatatg atagcgttta cactttttat attactcttc
    18361 gacattgata caggcaaata atctgtttac atttcagcca ttccagatgt cactactttg
    18421 gaagaaatga ttgtaataaa aatagataaa aaaccgatgg gactttgaca aaagtagtgt
    18481 caaagtccct ttttttgaat aaacggtgtt caaaagccat tttctcggta ttaagtcaaa
    18541 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttactactc gaaactaagc
    18601 gactttttca caacctctcg ataaaacggt gttcaatcag ctgaccctcg gtattagctc
    18661 aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga
    18721 acgttgactt cacagtctct tccttaaacg gtgttcaaaa acttgcatct gcggtaataa
    18781 gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    18841 cagggcaggt ttttcacgac ctctgattca cggtgtttct ttttcatcaa catttttacg
    18901 aaatactgtg gaagactatt gaaataagtg tggattgttt ctttgtgttt caatcgtttc
    18961 ttgccattct tcatttaatt ttggattacc gttttcttcc ttgaactcat aaggtcctaa
    19021 ttcaaaaatc ccagacagta tttgattcac gatttcatat agttcagtgt gatcagacac
    19081 atgattcttg aagatcatct cattatcacc acgtttgaac ctcacattcg ggctctcatg
    19141 atgaacctct atgcattttt ctcctttttt taactgacgg acttctgaaa acatctcata
    19201 aatatcttct ccagtaaaac cttcagcaag cgccagactg tccacaagac cgcctactgt
    19261 cagttcagag attattgctt gattggaatc taattttaat gtatagcttg tcgcttctac
    19321 tacgccacat gcattgcaac cgccttctga tagttcgctt tttctaatca attgaacttc
    19381 tttcattttc tcttcctcct cttaattttc tacttatgta tttttatcac ctgacatagg
    19441 tgactcctat caacgctatc atgtgctttt ttcctcagaa atcctattta tccattccat
    19501 ctatccctta ttcttctact tttctttctc tagcatataa gagatacgca tagtattcaa
    19561 gcaaggattg tcgatataaa aagaacaaat actgctattt tgatttcttt tggctatatt
    19621 acatataaat aaaatttatc atacctgctc tttttgacta tatcatcatc tgacttctat
    19681 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggtta aacttattaa
    19741 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatatggaa
    19801 tcactggtat gcttccaagt actgtccaaa cactggaaca acaatctgtt caagcttatg
    19861 gtcaatactt aagcaaacaa acagatttgg aaaaacgtat cttcttaatg aatctattca
    19921 atacgaatcg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
    19981 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
    20041 cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa catcaaagca agtttgaaga
    20101 acgcggcaga tggacgagac attcgtttga ttgttgtgac tgatgcagaa ggaatcttag
    20161 gtatgggcga ctggggtgtg aacggtgttg acatcgctat cggcaaattg atggtctata
    20221 ctgctgctgc tggtattaat ccagcacaag ttttgcctgt atcgatcgac gctggtacta
    20281 ataatgaaga attattgaat aatcccttat acttaggcaa tcgtcatgct cgtgtagaag
    20341 gcgaaactta ctatgaattc atcgatcaat tcgttcaatc agctaccgaa ttattcccag
    20401 aattgttatt gcattgggaa gattttggac gaggaaatgc tgcagcgatc ttagaaaaat
    20461 acgaagatca aatcactact ttcaacgacg atatccaagg tacaggaatt gttgtcctag
    20521 caggtgtatt aggcggattg aatatatccg gtgaacaatt gaaagatcaa ataattttga
    20581 catttggtgc tggtactgct ggtgtgggta tcgcaaacat ccttttagat gagatgatcc
    20641 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacaaggtt
    20701 tattgtttga agatacagaa ggattgactc ctggtcaaat cccattcgct cgcaaacgct
    20761 cagatttcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcgatccatc
    20821 cgacaatcat gattggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
    20881 aaatggctgc tcatacacca cgtccgatca tcatgccttt gtctaatcca acaaaattgg
    20941 cagaagcaaa agcaaaagat ttaatagaat ggacggacgg aaaagcatta gttggaacag
    21001 gtatccctgc tgctgatgtt gaatataatg gtgtaactta ccaaatcggt caagcaaata
    21061 atgctttgat gtacccaggt ttaggacttg gtttgattgc ttcaacagct acacgagtaa
    21121 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
    21181 caggagcagc tatcttgcct ccagtagcaa aaatcactga attttctcaa acgattgctg
    21241 aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg tgaagaaatc acggatatca
    21301 aagaagcagt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatc
    21361 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
    21421 ggcaaaacag cggccagcca actcctgctc ttttgtcgct tttctttggc ttttttcata
    21481 tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaact gcaactgcag
    21541 ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat caccggcgta agtttgccac
    21601 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttagggaaa ttaccgacga
    21661 atatgattgg accaatcgca gttttggtta ttttcggtaa tttatttcat tttatcggga
    21721 caaaaatccc tatcgtcaaa agttatttgg gtggtggttc cgtttttgcg atcttcgctt
    21781 ctgctgcgat tgctacattt gggatcttac ctgaatatgt agtgaaatcc acagaaaact
    21841 ttgtcagcaa tatgggcttt ttggactttt atattgctgc cttgattact ggtagcatcc
    21901 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
    21961 tttcaatggt cgtctccttt tttgccgtag gactcgtggg catgcttatc ggaaatggtt
    22021 ttgcaaattc cgttttatac atctcattac ctgcgatggc tggtggtgtt ggagccggag
    22081 ccgttccttt atcaacgatt tatgcaaatg ttttaggtac ggatgcttct tctatcattt
    22141 cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc tatcatcggt gcagcattag
    22201 ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa tggtaaatta atggaaaaag
    22261 gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc cattccacag ttaggtgttg
    22321 gtttgatggt atcattgact ttcttcatct taggacaaat tggtaactat tttgttccta
    22381 aagtccatgc ttacgcattt atgattatta tcgttgtcat ctgtaaaatc gccaacctat
    22441 taccagagta ttatgaagat gcagctatca tgttcaataa cttgattgtc aaaaacttga
    22501 cagcagctgt tcttgccggt atcggtattg cgttgctgaa tctgaatgta ttagcttctg
    22561 ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt tatcgcgatt tcagttgttt
    22621 caggatttgt cggtcgtttg tttggtttat atccaattga atcaactatt actgctggct
    22681 tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt cttatctgca gctaatcgga
    22741 tggaacttat tgcttttgct caaatgggca atcgacttgg tggtgccatc gttttgatca
    22801 tctctggttt cttgatgcaa ttactttctt agtcaaaaaa acaaaaatac ctgatcatca
    22861 agtatcggct tggtgatcgg gtattttttt gataaagatt tacaggattt tcaaaaatct
    22921 tttttgcatt ttatcggaat gataaccgat gtgttcataa aattggtgtg cttctactct
    22981 gtattctgca gaattaagcc ttattgtcgt tattcctcgt tttttcgctt cttctacgcc
    23041 agtcatcaaa gcctttccga tgcctctttt ttcagcatat ttagacacag caagtgccat
    23101 aatattgaac attggatcgc taaaaatcga ttcataggac tccgcttgta catagccagc
    23161 tgctttttta gaagtaggat cttcataaac caagaaaaaa tttttctctg gctccttcag
    23221 cagtttcttc atttgtctca ccgtaacggt taatggtata tcatacccta attgatccgc
    23281 attgatttca cgtagtgctt ctgcatcttc taaacgcatt tcccgtaaca tccaaatcac
    23341 cagccaattt ctgatttttt aaaatgaatt tttttatcat agtatactct ttttttcatt
    23401 tagaacaata tgtttcagtg attttgactt gcgttagtct agattctcct aatgtttgta
    23461 agatgacaaa ggcattgtaa caattgacca aaagaaccac gaggacaatt acttgaaaaa
    23521 taaaggtcac tttttttgga gacaaaaccc tgctaaatct tgctccccac aaacctccaa
    23581 taacagcagc ggggataaca taaaacaaca tagataaatc aaagcgcata aagctacttg
    23641 ttaaagcaat cgttaccaat tttgccagtt gagaaaagaa aatcgtagat agagaatata
    23701 gtgttgcttc ttttattggc aatgcaaaca tcagcatcaa caaagaaaca ttgactggtc
    23761 caccaccaat acctaaaaaa ctagctaaaa agcccagaac ccccccacat ataaggtacc
    23821 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
    23881 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
    23941 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
    24001 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
    24061 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
    24121 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
    24181 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
    24241 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
    24301 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
    24361 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
    24421 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
    24481 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
    24541 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
    24601 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
    24661 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
    24721 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
    24781 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgtatt gaatacttct
    24841 tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct
    24901 tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagca
    24961 ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagtaggatt
    25021 tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcatgg
    25081 tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta ttaaaataat
    25141 tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa tttatcgatg
    25201 actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga
    25261 acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc tttgatcttt
    25321 tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat atttttaggt
    25381 aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa ccgatcaaag
    25441 ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc tgtccagcca
    25501 aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc cattactctc
    25561 ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg atctcatcgc
    25621 gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag gccgggtctc
    25681 cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc
    25741 gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac
    25801 gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc
    25861 ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa
    25921 cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct gtagctttat
    25981 caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt ttttacacaa
    26041 ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg tttttgttgc
    26101 ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa agacattgaa
    26161 ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg ctaccagttc
    26221 attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt ctttgatata
    26281 tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac gtgtccctgt
    26341 catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta aatggctgcg
    26401 attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg ggtcaatata
    26461 caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat tggcttgtcc
    26521 tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg aaaggctaag
    26581 atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat gttttccatc
    26641 atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa tagttgtaat
    26701 ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc ctgcaatcac
    26761 agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta
    26821 ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag aaagaaatag
    26881 aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa gagacggatt
    26941 cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt caaaaatttt
    27001 cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg cgcaaaagct
    27061 tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt ttgcttggct
    27121 gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg gtgacgggaa
    27181 tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac acccgcaata
    27241 caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt tacgcttctg
    27301 gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg ctatgacggg
    27361 aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca tgatggaaag
    27421 caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag cttctggaaa
    27481 tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg atcgtacctc
    27541 gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt
    27601 aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac tttactgcga
    27661 actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc
    27721 ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg atttacctgt
    27781 gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga aattttttag
    27841 aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg ctatctacag
    27901 cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg tgatgccttt
    27961 agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac attatgtctc
    28021 aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt ttgcgattgc
    28081 ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga tatttattca
    28141 gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg tacctaggcg
    28201 ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact ggatctgtgt
    28261 tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag tagcattcat
    28321 cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata ttcccgggat
    28381 ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg ttactgaatc
    28441 ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag caatcgcttt
    28501 aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag taaagcgtga
    28561 cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta acaaagaaaa
    28621 ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa cattgcgaca
    28681 agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag
    28741 cttctcacat ttttacataa aattgactta atactgagaa actgactttt gaacaccctc
    28801 tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct ttattcaaat
    28861 tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat tagaactaaa
    28921 ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat tccgtataag
    28981 acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa acccaaactt
    29041 cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag caaccgataa
    29101 tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc aaatagcaag
    29161 ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc gatcccacca
    29221 gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata ttggttcata
    29281 gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat aagattgctg
    29341 ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag gaaaacaaca
    29401 ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc aaaagcaaga
    29461 ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa attaggttct
    29521 tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt ctttttatgg
    29581 gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag ctttggtact
    29641 atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat tccccaaccc
    29701 aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa taatatcgaa
    29761 atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt catccacatg
    29821 attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac tgtttttcta
    29881 tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa
    29941 agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag caaaatccct
    30001 agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac aaaaggaatg
    30061 atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga
    30121 ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac tgatatactt
    30181 tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc tggcaattta
    30241 acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa
    30301 agattttgag acaaaagata gatagttaat cgctttccct tttttccttt ctctcttgct
    30361 ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc taataattgt
    30421 cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca aataaatctt
    30481 ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct gataaatcaa
    30541 ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat ccaatacatg
    30601 tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc
    30661 aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa tatctggatc
    30721 tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat
    30781 tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta aacgcttttc
    30841 ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt ctccagccgt
    30901 caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt tgatactcaa
    30961 tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg gttcaataat
    31021 actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta aaaatccaaa
    31081 aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt tcccgtaact
    31141 tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta ttacttttta
    31201 ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag
    31261 aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct tctaacaggt
    31321 aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct tgcataaaat
    31381 tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt gcaaatgtta
    31441 ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata taagctttta
    31501 aatctttaca taaatactca tattcgagtg attttttata ttttttaatt gtgccgttta
    31561 cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct ggcatgctca
    31621 ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa cggacagttg
    31681 tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc aataagtaac
    31741 tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa ccactaggcg
    31801 taatctttcg attaataaca gttattgctt tattcggatg agacgttaga taaacaattt
    31861 tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta taataggcgt
    31921 tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt ttaatgaatt
    31981 caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga cttaataaaa
    32041 ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta tcatccaaaa
    32101 taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc aaaatcattt
    32161 catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac aatcttttcc
    32221 aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat aaatatcaaa
    32281 caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg tcagctgtct
    32341 gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg atttttcgga
    32401 gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt gaatggttga
    32461 taatagtctg attggatggt tccttctatt ttataccatt catcattttt aaccgttact
    32521 ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca atggataatg
    32581 ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta aacaaatcct
    32641 tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag ctccataact
    32701 tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt ttctttcaac
    32761 tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc cggctttaag
    32821 tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa attaaatcct
    32881 ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc cacgattact
    32941 ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa cccatggtca
    33001 tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa atacagctga
    33061 actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg ataatggaca
    33121 ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc gaaataccca
    33181 gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga atccaataac
    33241 gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat atcgtttcat
    33301 catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag ctacaggtgc
    33361 tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg agcaaagaga
    33421 catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg agccgagcgt
    33481 tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc cgccaatgat
    33541 caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat gcccagtttg
    33601 atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat ggacatgatg
    33661 atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt ctttttgaaa
    33721 ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca cccgccagct
    33781 tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga ttggattgat
    33841 gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt ttttaacaaa
    33901 ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc ctaagatcga
    33961 accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac gttctggggt
    34021 caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag gtaatgcttc
    34081 aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa taaaagaaaa
    34141 catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg cttttattca
    34201 aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca aagcacttca
    34261 attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact tctatcataa
    34321 aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac atactgcaac
    34381 agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt cacgctgttc
    34441 gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat ttggatgcat
    34501 ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt gtcctgattt
    34561 ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa aaaatccgtt
    34621 atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa atcttccgaa
    34681 caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt ttgccttcat
    34741 ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat ttgcgtcaga
    34801 atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt ggatcaatac
    34861 atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga ttcctgcttc
    34921 cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa ttgctacttc
    34981 gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg ggataccgtc
    35041 tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac gtatgctatt
    35101 actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac cgatcagcgc
    35161 aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa gatcttctac
    35221 atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc gacggatcgt
    35281 tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt tctttccagc
    35341 attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg tcgcctttgg
    35401 aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc tttgatttct
    35461 ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt gtgaaatttt
    35521 attctttatt catttattta tcatttctgc aaatctcgca gtataatgga atttatggta
    35581 gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag aaaagaggat
    35641 tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga agtcttattt
    35701 gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg cagaaatggc
    35761 gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga tgaaggaaag
    35821 atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg acttgattcc
    35881 aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca aatggaagag
    35941 cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc tgcttatagt
    36001 tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa cggctatggt
    36061 tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc attttatgat
    36121 cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc tcgcatgctt
    36181 ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga tatcgagaca
    36241 ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat tgtttcccat
    36301 gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag aagaaaaatg
    36361 cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca gttagcaagt
    36421 gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga cttcgttgct
    36481 cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa gacattagaa
    36541 aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt tctattcagt
    36601 atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat cggatatgat
    36661 aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc gattgctcta
    36721 gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca ccagatccct
    36781 tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta cgatcaaggt
    36841 caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga gcatcctacc
    36901 actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg cgaagatgta
    36961 gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt agccaagtta
    37021 gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt agatattgac
    37081 aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct ttttgtttcc
    37141 catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc tgaaaatggc
    37201 agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga agaagaagaa
    37261 atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac gaacaaatct
    37321 gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg aaaaattact
    37381 caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga acaagaaatg
    37441 gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca agatttagaa
    37501 gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag cttggaatta
    37561 gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg ttttggtttt
    37621 tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa taagaagacc
    37681 aattactagt cattctcact aaagcttcta aataatagta atcaccccaa atacaaggtg
    37741 tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag ccatcttctt
    37801 cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca gcagactggt
    37861 ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat aatccacaaa
    37921 ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca aagatcaaat
    37981 cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct aatattttgc
    38041 ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa ccgtaaatcc
    38101 cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc gtacttccat
    38161 atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa ccatcttctc
    38221 ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta tatttttgat
    38281 ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa tccatgatca
    38341 ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact cctgccttct
    38401 cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc atttttcgat
    38461 ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca agatcgtgat
    38521 gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct ttttcagctg
    38581 tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt cctgtccaaa
    38641 atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata ggacactcgc
    38701 ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg tgtctgcgac
    38761 aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat tcatttatcc
    38821 atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc ccaccagcct
    38881 ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc cggttgtccc
    38941 atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc gatcttgatg
    39001 agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct ctaatccagg
    39061 aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa tagctgtgga
    39121 ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag cttcactcac
    39181 cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag aacattcaat
    39241 agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt gtacgcgaat
    39301 ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag taggttccca
    39361 tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc gaacgatgaa
    39421 ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg tgtccgctcg
    39481 ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg ctcgcggtat
    39541 cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg tcaatgtatg
    39601 gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat catgttgtat
    39661 gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag gcatttcttt
    39721 agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct tattcagcca
    39781 taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt aggtataacc
    39841 aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc ttatattccg
    39901 attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc acaacatagc
    39961 tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag gcacatttgc
    40021 accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc ggttcttata
    40081 tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat aataatgaaa
    40141 tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc agccattttg
    40201 aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt taactggata
    40261 accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat taccatctgc
    40321 acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt ctcctgtatc
    40381 taattttttc cataataatt gttcatgatg atataacgct aataacattg cgggcatttc
    40441 taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg aaggatctgt
    40501 tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt ggtcaattgc
    40561 gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt tatttccata
    40621 tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa acggctggag
    40681 tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta atagtggtac
    40741 tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc cgactgttct
    40801 aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac attataagtc
    40861 agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc catattccaa
    40921 tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg atacattttt
    40981 gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc gcatactcca
    41041 gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat ataaccagtt
    41101 ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag atagtacgga
    41161 ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt tcctctgctc
    41221 caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt ccctgattct
    41281 gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata cttagctgtt
    41341 gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc cctcgaagcc
    41401 caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg tttatggtca
    41461 ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac cgtagctgca
    41521 tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc aatagccgat
    41581 ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag ggcagattct
    41641 aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt cttgtgaaag
    41701 agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc tctgtccttt
    41761 attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc cattctgtct
    41821 aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca ttccttcgtc
    41881 tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag ttgctcctag
    41941 aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc caatgtcatt
    42001 ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca ctgaagttgc
    42061 taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa ccgtcatatt
    42121 tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta atggaaatag
    42181 atattgattc caacttccca taaagataag taaaccacta gtaataatcg taggtgataa
    42241 aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga ttctggcagc
    42301 ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca ctgaagttgt
    42361 actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta aacgactcat
    42421 aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata gcataactgt
    42481 attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac ccgccattgt
    42541 agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat tccaaataat
    42601 caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca tatccccaaa
    42661 cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag ctacaatcat
    42721 atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt acgtaaaaat
    42781 tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat tgaatagccg
    42841 ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt ccaaaatcaa
    42901 ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa ttttctggtc
    42961 cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca ttgatagtgg
    43021 ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat aaaaagaatt
    43081 gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta gaaatccctt
    43141 gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc atcgtgatga
    43201 tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta attcctagcg
    43261 cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat acggaagcta
    43321 cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat ccttttcctt
    43381 ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc gttattggaa
    43441 ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat ggatctcgga
    43501 agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt ttcataaaac
    43561 taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa ataacaaaag
    43621 gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt ttcttgctgt
    43681 tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc taatattttt
    43741 taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc ttttagatat
    43801 gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa ataagacgta
    43861 gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat tttattcaat
    43921 agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc ttctgtttga
    43981 taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa gcattcttca
    44041 tctgtcagca taaatgtaag aaattgttgt gccacttgtg gatttttggc atctttaggg
    44101 acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt tgaaaaagga
    44161 atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga catattcgta
    44221 gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc agcatcttgt
    44281 gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt tgcagcatgt
    44341 ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga ttgattctgc
    44401 tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc aattccaaaa
    44461 ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca agaactgatt
    44521 tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca aagtgctggg
    44581 gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg caaaatattc
    44641 aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc atcagcacta
    44701 taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc catggagata
    44761 atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac tgttgctgtt
    44821 cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg gttatagatt
    44881 tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac ttctaatgta
    44941 gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt agtttttcca
    45001 catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa tttgtatctt
    45061 tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt tcacttatgt
    45121 atattaacgg aattagtaac acaaatattt atataaaaaa atattttact tgtcgttttg
    45181 gcttttc
//
LOCUS       DAFELO010000015        44724 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFELO010000015
VERSION     DAFELO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.015"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="LP452_000933"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="LP452_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL2013681.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="LP452_000934"
     CDS             complement(324..893)
                     /locus_tag="LP452_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL2013682.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="LP452_000935"
     CDS             1079..1981
                     /locus_tag="LP452_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2013683.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="LP452_000936"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="LP452_000936"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL2013684.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="LP452_000937"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="LP452_000937"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL2013685.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="LP452_000938"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="LP452_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL2013686.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="LP452_000939"
     CDS             8549..8734
                     /locus_tag="LP452_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL2013687.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="LP452_000940"
     CDS             complement(8796..9992)
                     /locus_tag="LP452_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2013688.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="LP452_000941"
     CDS             complement(10162..11598)
                     /locus_tag="LP452_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL2013689.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="LP452_000942"
     CDS             11891..12700
                     /locus_tag="LP452_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2013690.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12886..15039)
                     /locus_tag="LP452_000943"
     CDS             complement(12886..15039)
                     /locus_tag="LP452_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL2013691.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15434..16282)
                     /locus_tag="LP452_000944"
     CDS             complement(15434..16282)
                     /locus_tag="LP452_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2013692.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16506..18446)
                     /gene="thrS"
                     /locus_tag="LP452_000945"
     CDS             complement(16506..18446)
                     /gene="thrS"
                     /locus_tag="LP452_000945"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL2013693.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18783..18962)
                     /locus_tag="LP452_000946"
     CDS             complement(18783..18962)
                     /locus_tag="LP452_000946"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL2013694.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(19009..20832)
                     /gene="cadA"
                     /locus_tag="LP452_000947"
     CDS             complement(19009..20832)
                     /gene="cadA"
                     /locus_tag="LP452_000947"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL2013695.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21110..23389
                     /locus_tag="LP452_000948"
     CDS             21110..23389
                     /locus_tag="LP452_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2013696.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23482..24129)
                     /locus_tag="LP452_000949"
     CDS             complement(23482..24129)
                     /locus_tag="LP452_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013697.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24308..25027)
                     /locus_tag="LP452_000950"
     CDS             complement(24308..25027)
                     /locus_tag="LP452_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2013698.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25158..26309)
                     /locus_tag="LP452_000951"
     CDS             complement(25158..26309)
                     /locus_tag="LP452_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL2013699.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26526..27266)
                     /locus_tag="LP452_000952"
     CDS             complement(26526..27266)
                     /locus_tag="LP452_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2013700.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27270..27593)
                     /gene="rsfS"
                     /locus_tag="LP452_000953"
     CDS             complement(27270..27593)
                     /gene="rsfS"
                     /locus_tag="LP452_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL2013701.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27650..28243)
                     /gene="yqeK"
                     /locus_tag="LP452_000954"
     CDS             complement(27650..28243)
                     /gene="yqeK"
                     /locus_tag="LP452_000954"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL2013702.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28227..28877)
                     /locus_tag="LP452_000955"
     CDS             complement(28227..28877)
                     /locus_tag="LP452_000955"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL2013703.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28887..29198)
                     /gene="yhbY"
                     /locus_tag="LP452_000956"
     CDS             complement(28887..29198)
                     /gene="yhbY"
                     /locus_tag="LP452_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL2013704.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29214..30323)
                     /gene="yqeH"
                     /locus_tag="LP452_000957"
     CDS             complement(29214..30323)
                     /gene="yqeH"
                     /locus_tag="LP452_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL2013705.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30320..30847)
                     /locus_tag="LP452_000958"
     CDS             complement(30320..30847)
                     /locus_tag="LP452_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL2013706.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(31027..31812)
                     /locus_tag="LP452_000959"
     CDS             complement(31027..31812)
                     /locus_tag="LP452_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL2013707.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31805..32674)
                     /locus_tag="LP452_000960"
     CDS             complement(31805..32674)
                     /locus_tag="LP452_000960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL2013708.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32675..34054)
                     /locus_tag="LP452_000961"
     CDS             complement(32675..34054)
                     /locus_tag="LP452_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL2013709.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(34062..34481)
                     /gene="fabZ"
                     /locus_tag="LP452_000962"
     CDS             complement(34062..34481)
                     /gene="fabZ"
                     /locus_tag="LP452_000962"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL2013710.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34506..34982)
                     /gene="accB"
                     /locus_tag="LP452_000963"
     CDS             complement(34506..34982)
                     /gene="accB"
                     /locus_tag="LP452_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL2013711.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34985..36220)
                     /gene="fabF"
                     /locus_tag="LP452_000964"
     CDS             complement(34985..36220)
                     /gene="fabF"
                     /locus_tag="LP452_000964"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL2013712.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36239..36976)
                     /gene="fabG"
                     /locus_tag="LP452_000965"
     CDS             complement(36239..36976)
                     /gene="fabG"
                     /locus_tag="LP452_000965"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL2013713.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36988..37905)
                     /gene="fabD"
                     /locus_tag="LP452_000966"
     CDS             complement(36988..37905)
                     /gene="fabD"
                     /locus_tag="LP452_000966"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL2013714.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(38012..38236)
                     /locus_tag="LP452_000967"
     CDS             complement(38012..38236)
                     /locus_tag="LP452_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL2013715.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38289..39251)
                     /locus_tag="LP452_000968"
     CDS             complement(38289..39251)
                     /locus_tag="LP452_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL2013716.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39261..39701)
                     /locus_tag="LP452_000969"
     CDS             complement(39261..39701)
                     /locus_tag="LP452_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2013717.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            40007..40972
                     /locus_tag="LP452_000970"
     CDS             40007..40972
                     /locus_tag="LP452_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL2013718.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(41029..41259)
                     /locus_tag="LP452_000971"
     CDS             complement(41029..41259)
                     /locus_tag="LP452_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL2013719.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41345..42232)
                     /locus_tag="LP452_000972"
     CDS             complement(41345..42232)
                     /locus_tag="LP452_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL2013720.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42321..43544)
                     /locus_tag="LP452_000973"
     CDS             complement(42321..43544)
                     /locus_tag="LP452_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2013721.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43561..44607)
                     /locus_tag="LP452_000974"
     CDS             complement(43561..44607)
                     /locus_tag="LP452_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2013722.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT        12650 a         9672 c         7747 g        14655 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
    12781 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
    12841 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
    12901 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
    12961 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
    13021 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
    13081 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
    13141 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
    13201 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
    13261 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
    13321 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
    13381 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
    13441 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
    13501 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
    13561 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
    13621 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
    13681 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
    13741 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
    13801 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
    13861 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
    13921 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
    13981 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
    14041 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
    14101 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
    14161 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
    14221 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
    14281 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
    14341 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
    14401 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
    14461 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
    14521 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
    14581 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
    14641 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
    14701 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
    14761 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
    14821 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
    14881 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
    14941 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaacca
    15001 agaagtattt aatatttttt tcataaaatc tttcatcatt tcctatcctt ccgcctattt
    15061 acattcatgc ttcattgtac ccaaaaactt cttaataaac aagaagtatt taatattttt
    15121 ttcataaaat ctttcatcat ttcctatcct tccgcctatt tacattcatg cttcattgta
    15181 cccaaaaact tcttaataaa aaagaaaaaa tgggcggagc aagaaattct ttacctaatt
    15241 tcaagataaa atcaactttt tgtttcacag caatcactta aagttacata tccaactatt
    15301 tttttcatta aaaaataaac ttatttttga aaattatgtt ttcttaagtc gtgaaacact
    15361 ttgatggatc atttatttgt ggaaaactac gttttgttat tctaaaaaac ttatataaaa
    15421 aatagtaagg tttttatttc gaatccataa aatattcttg gattgcttct cgtgtttttt
    15481 tcactttttc gctaagcttt tcgtttttag ccatatagaa ataaaaaatg atatcgatcg
    15541 tagcaaattg ggccaaaagt gaattagttg ccgcacttcg attcgtgatt tcttttggtc
    15601 tagatgtttg tacgagtaca tcggctatct tgctaagcgg gttatttcca aattgcgtca
    15661 acgcaatgat ttcgatattc atgcttttcg ccagctcggc taatgcgact acatctgcac
    15721 ttctacctga gtttgaaaca agccaaagca ctttcttttt ctcattgctg acaagttgcg
    15781 ggagcaagac gtggatatct ttttcaaaaa tgattggctt accgacacga gaccattttt
    15841 gcagaatatc ttctactact aaagaagagg cgccaacccc atataaaaaa acagtctctg
    15901 ccgattccaa acactctacg actttttgga tcgtttcttc ttctagaata tctactgttt
    15961 cttttatcgc tacttgtgca ttgttaccaa gtttgttttt gatagagtaa aaggactcat
    16021 ttgcttctat atcactaaaa ccttcatcga ttccagtctg tagttctgtt gaaatcgtga
    16081 ttttgaagtc cgtcagactt tgaaaaccga tttttttgga aaaacgtaca actgtcggcg
    16141 cactcaaccc ggaagcttct gctaattcat tagctgtcat tgtagggacc ttctcaatat
    16201 taccgataat gtagttggcc aacttttttt ctgctgaaga ataatcttcc atcatacttt
    16261 caatacgact aattacgctc ataagttcct ccaccctttt aatgatagtc tatgtaagta
    16321 taccatatca gacccttatt cacggtcgta tactggataa aaaacaaaaa aacaatccgc
    16381 ccaacagaat atcttatccc atcaatccca cagattctat tgagcatctg tgtctttgag
    16441 gaatacctgt tatctattgt tcggattgct ttctatctac tactttttta caggaaattt
    16501 acctattagt tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata
    16561 ttcaaatctt ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct
    16621 tccaattcct tgtctccaac aactaattgg taagggatct tttgtgtttg agaggcacgt
    16681 atcttatagc ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc
    16741 aaacgttctt tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt
    16801 gttgcttgta taggtgccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa
    16861 gcgacaaaac gttccattgt agaaacaatt ccgcggtgga taactactgg acgatgcgta
    16921 ttttccccgt cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat
    16981 tgtatagttg ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt
    17041 ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata
    17101 gcggctttca gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct
    17161 gtattgtttg gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca
    17221 tatactgcca ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga
    17281 acaaatgtat gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca
    17341 cctgattttt catagcgatg catcattcct aattcagcga tacgaattgg taattcacga
    17401 taagaatgga tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tgggcgaaga
    17461 acaagcattt cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc
    17521 cagtgtccag atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg
    17581 taacctagac taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc
    17641 ccttttggaa gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca
    17701 agttcttttc ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat
    17761 tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg
    17821 tttgagtttc cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg
    17881 ccagtggatg gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca
    17941 gtaatcactt cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca
    18001 aaaatttcta atgcttcctg ttttgaaaga acacggcgtt cgatcggcag attttctttg
    18061 acgattttca tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt
    18121 ccattatctg tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata
    18181 tttggataga ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag
    18241 ccaagtgcat cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga
    18301 cgagtcaaat caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt
    18361 tttgaaatgc tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca
    18421 ccatctggaa aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca
    18481 gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga
    18541 aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt
    18601 ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga
    18661 aaagtttcag cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc
    18721 atcaatcaat tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa
    18781 acttattttt tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg
    18841 acatggatgt gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc
    18901 atcatatttt ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc
    18961 ataatgctct ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt
    19021 tgtcttgggt actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa
    19081 aatagagacc gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc
    19141 cgtacttgca attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt
    19201 cgctattctc atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc
    19261 tttcaagatg accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc
    19321 aataccgata tctgcagcag caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc
    19381 aacaggtctt tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct
    19441 acgggcatgt acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc
    19501 atccccggtg atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt
    19561 tgctgcttct gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat
    19621 tgttacatag attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg
    19681 tccaatcttt acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt
    19741 cagttgacta acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa
    19801 gatatggcta gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat
    19861 tccattgact ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt
    19921 atcaaaagca atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac
    19981 gatcccgttt ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat
    20041 cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa
    20101 ccaccaagaa ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc
    20161 agctaaacgg acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat
    20221 ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt
    20281 taaagaccct gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt
    20341 caaagaagat tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag
    20401 ttcgccaggc ttgactagta actggtcatc aatatttatt tcatcgatta ctatatcctg
    20461 caattttcct tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag
    20521 ttctttaccc gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat
    20581 caatatcatc aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat
    20641 cgctaataca tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat
    20701 caagaaggcg agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg
    20761 aaaaacaaat tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt
    20821 gaaatgtgtc attgtttttt ctcctctttt atgtgactta gagaggttag taataaattt
    20881 ccagctgcct ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc
    20941 ttaattgaaa acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa
    21001 atattcagca aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac
    21061 tatgctatac ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa
    21121 acaactagaa caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat
    21181 gctggataca aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc
    21241 taatcacaag attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg
    21301 ccaacatgag caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct
    21361 gcaaacgttg tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga
    21421 ccgtgaaaca gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac
    21481 aatcgttatt gactggcgcg caccaattgc caatctttat tatgaaggtg agattggtcc
    21541 cgcattttat gaaactgaaa cagatagaat agatgtcgat ctgcagttga aacgacaatt
    21601 taaaatccaa gaaggacgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga
    21661 ctttttgcta gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac
    21721 gatacaaaaa gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga
    21781 aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta
    21841 tcataatcgc aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt
    21901 ttctgattac atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac
    21961 attcaaaaat tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca
    22021 agaaagtggt tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac
    22081 cttagtcgat cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg
    22141 tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa
    22201 agaaacaaat gaacaattac ctttacatca tagactttct ctattacaaa cgaagctcct
    22261 gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc
    22321 ggaagaacag ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa
    22381 ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat
    22441 ccatcgaggg atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct
    22501 acaaactgtg ccactttctc ttctaacacg acataaaatc agcgagacca ggtggcagga
    22561 acatttgaca actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt
    22621 actattcttt ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta
    22681 tatctttatt gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt
    22741 gttcccaaaa gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga
    22801 aacgatcgtt ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt
    22861 aacgacaagc tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa
    22921 agaacatcaa gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac
    22981 aacagatgat atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc
    23041 ccggtattgg cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc
    23101 tcaactgcca gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat
    23161 gaagcgttcc attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt
    23221 tttcttgtgg gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta
    23281 caccatgtgt acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc
    23341 tttgttaaac aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca
    23401 actgctatca ttaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt
    23461 aattcaatca tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt
    23521 taatttgaca agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc
    23581 aaataccttg ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca
    23641 ttcaatgaat tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat
    23701 ctctgataca cgaaatgcta ggtatgcact atcgatcact ttattttttt tcgtgtctat
    23761 cttctcattt ctttcgataa gaattttttc aggacgatgt ttcaagctcc actctaatag
    23821 tagaaagaaa gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg
    23881 acgaaaaagc agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac
    23941 catacactct aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc
    24001 ttcaacgccg aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc
    24061 taactcttct actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt
    24121 tgcttccata ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt
    24181 atgctctcat cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag
    24241 acaatctgtt agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac
    24301 tggttgctta gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt
    24361 ccagtgcatc aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt
    24421 tttgagggat catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg
    24481 cttcttgtat actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt
    24541 ccattacatc gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt
    24601 cttctcctgc aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa
    24661 cacccatgtt cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca
    24721 cattgttcgt caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc
    24781 gcaattcaga gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa
    24841 tatgttttgg tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg
    24901 catgcggatc aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat
    24961 aaatccggct attattagca tctttagcag ttttctttgc tacgatattt gcccatttac
    25021 gtcccatatt ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt
    25081 ccattatact tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct
    25141 catttttagt caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata
    25201 ttgcctccca attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct
    25261 ttttttccga ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca
    25321 gcatcggtaa atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc
    25381 tttttgatat tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt
    25441 tttgttttta cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc
    25501 atgcgcgatt ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa
    25561 gataacaacc gataacgcaa atacggccaa taagattccc aagtctgcag cgaacaagtt
    25621 gaaagatctt cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac
    25681 gcatggcgaa tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag
    25741 cctgcacttt tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc
    25801 tttgcataac ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga
    25861 tacagactgc gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt
    25921 tcttgaaatg cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg
    25981 taatcaaacg aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc
    26041 aagattttta ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga
    26101 agctccacta ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg
    26161 atgatagccg gttcgcctct ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct
    26221 gctcctgtta tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg
    26281 tactctacga taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa
    26341 agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact
    26401 attctcgcaa acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat
    26461 cgttcggata tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa
    26521 aaccgctagt ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg
    26581 tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa
    26641 taactttcca atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca
    26701 gacccttctt gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt
    26761 cctggataac tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct
    26821 ggaaaaacct catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct
    26881 tctaacgctt gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag
    26941 cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc
    27001 atattgcctt ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc
    27061 atcaacatct cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca
    27121 accgctaatg ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa
    27181 tgacgtttcg aaaaagctaa ccattgctga taaagtgttt cgtccatcac ttcatcgtaa
    27241 atgaaagcaa atgtttcata agccatatgt taatccaacc attcactaag atcgactagc
    27301 ggagcatctg accatagttt ttccagatta tagaaatttc gttctggcgc atggaaaaca
    27361 tgtacgatca catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata
    27421 cgttttactt catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg
    27481 atttgacgtt cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt
    27541 cgaacgtcta aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aatcgcaatt
    27601 tttaattgtt ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg
    27661 ctacccagta attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca
    27721 aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa
    27781 tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat
    27841 aaatgatttt gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat
    27901 gaagaatctc tgcatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac
    27961 catgccagat tgcattatta taattcaata actccggatc aaaaccgtct cttacgatta
    28021 ccattttgaa ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac
    28081 tagctttttc aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc
    28141 caagcacatg cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt
    28201 ctctagaata agcagtatat tcattactat agtccatatt catacaaccc ttcattttga
    28261 atataatcaa ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt
    28321 tgacgtattt tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat
    28381 ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc
    28441 tcatcgattt tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa
    28501 taatctgtat ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc
    28561 ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc caccgctaat
    28621 tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc
    28681 tgataagaag gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc
    28741 atcactaaat gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt
    28801 ttcttctcta agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta
    28861 gcttgcgtat tcatcatggt cactccttat attgctttta cttcttttga tagcctttga
    28921 tacttttctt ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt
    28981 tcgcaatcta tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt
    29041 tgaagtaacg atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc
    29101 attgctgaat tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg
    29161 ctacgtaaaa aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc
    29221 aatgctttgc gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc
    29281 gctgttacag tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa
    29341 aactcaaaac gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat
    29401 aatccaccta catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata
    29461 tttacatcat ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat
    29521 cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg
    29581 atttctttta acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga
    29641 tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg
    29701 attttatcca aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct
    29761 gtatttttga tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata
    29821 tagacatccc gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga
    29881 gatttcttag cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt
    29941 tccttcatcc attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg
    30001 ttcccaacta aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa
    30061 ccattgaagt caaaaatatc caccacattg acgatcaatg catctttttg ccctaattca
    30121 ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat cttgggtctc attataatgg
    30181 cgtaatctga aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc
    30241 gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg
    30301 atacattgta attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg
    30361 ctaatagata ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat
    30421 ctgttgcgat aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta
    30481 tatcagtcat aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt
    30541 tctttgctat gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat
    30601 caaatttttc aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag
    30661 ggattcctgc atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat
    30721 tattccatgc aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct
    30781 ttaattgggc aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg
    30841 aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc
    30901 cgtattatac tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa
    30961 agcatttctt tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa
    31021 aaacaactaa tattggcgga acctcttgta tcgtttttcc agtaatttat gctgatctaa
    31081 tcgaacaagt tccgtcaatc tttcaatcaa cgcttttttc ataatgtgtg cgactttata
    31141 tcgtggcaac tgctcgccgt tgaacgtttc aggaataact cgatcaacga ttcctaactc
    31201 gcgtagttct ggcgcagtaa ttttcatcaa ctcagctgct tctgcggcgc gactaccgtc
    31261 tttccacaaa atcgacgcaa atccttctgg agaaagcaca gcataaatcg tttcttccag
    31321 catccagact tcatccgcta aacctagagc aagggcaccg ccacttccac cttcaccaat
    31381 gatgatggcg atgatcggta cctttaaatc agacatttcg agcagatttc tggcaatagc
    31441 ctctccttcg ccgcgctctt ctgctcctac accgcaatat gcacctgctg tattgatcaa
    31501 ggtaatcacc ggtctattga acttttccgc ttgtttcatc agtcgtaaag cttttcgata
    31561 gccttcagga tggggagaac caaaatttct gcggatattt tcctgcagat cccgtccttt
    31621 ttgaataccg atcactgtta ccggtttgcc atctaatcga gcgattccac caacaatcgc
    31681 ttcgtcatct gcaaataaac gatcaccatg gcattcctcg aaagaatcga agatctcttc
    31741 aaaaaaatcc aagcttgtcc atcgttcttt agctcttgct aactgaacga catcatgtgc
    31801 tgtcttatcc acgatctatc catcctttct gtgtatgcaa ttcaagaagc gtatggattt
    31861 tctgtttcaa ttccatgcgg ggaacaatct gatcgacaaa accatgagat aataagaatt
    31921 cagccttttg gaaatcttct ggaagttcct gcttgatcgt ctgttcgatg acgcgtcttc
    31981 cagcaaaacc gatcaagctt tggggttctg ctagaataat atcgccttcc attgcaaaac
    32041 tggcagttac tcctccggtc gtcgggtccg ttaaaacagt tagataaaaa agaccttcat
    32101 tgctatgccg tttgacagca gcagagattt ttgccatctg cattagagaa aagattcctt
    32161 cttgcatccg tgcgccacct gaagctgtga acaagacgac tggaagactt tcctttgttg
    32221 ctcgttcgaa taatctggtg atcttttctc cgacaatcgt tcccatactt cccatgataa
    32281 aattaggatc catcactcca ataatgaagg gaatcccctg aatggttgct ttaccagtga
    32341 ggactgcttc atgaagtcct gttttatctt gcattttttt aattttttcc gtatattcgg
    32401 gaaatgctag cggatctttg gttacaagat ccgtatccca ttcttcaaaa cttttttcat
    32461 caataatcaa agctagacgc tgccatgcac cgattcgaaa agcgtatccg caatgagggc
    32521 atactttttc ttcaccgatg tctttcgtat aaaggatatg tttgcaattt ggacactttg
    32581 cccacatgtt atcaggaaca gatggcgagt tagcaggttt gctctctcta ttaggattga
    32641 tccgaatata tttctttttc ttaaacagag ccatctatgc gcctcctatc tccgtttctg
    32701 gtgtccaatt cggtaaaaat tcttcttgta aaaatgctgt gctgtaatca cctgctatga
    32761 ctgcaggatg actaatcaaa tccatctgaa attctgcgtt cgtgatgatt ccttctgtga
    32821 taaattcact taaggctcgc tgcatcttca ttaatgcttc aaaacgcgta tttccatgga
    32881 cgattatttt tgcaatcatt gaatcataat atggcgggat cgtatatccc gggtaaactg
    32941 cactgtctac tcgcagtccc atcccacctg ctggaagcag taagttttgg atcttccctg
    33001 gagaaggagc aaaatggaaa gcaggatttt ctgcattaat ccggcattcg attgcgtgtc
    33061 cttggaatac tacttcttct tgacacacat tcaaaggctc accggctgct atctccacct
    33121 gtttttttac taaatcgatt cctgtgacca tttctgttac tggatgttcc acttggattc
    33181 gagtattcat ttccatgaaa taaaaatcgc cttcttcatc catcagaaac tcgatcgttc
    33241 cggcattttc ataattgact gctttagctg ctcgaacagc agcgtctcct agtgcttctc
    33301 tttttgtttg tgagatcact acagaaggag attcttccag tactttttga ttgttgcgtt
    33361 gtaaagaaca atctcgctcg cctaaatgaa tgacatgacc aaatcgatca cctaagatct
    33421 gaacttctat atggcgagca ggataaatga ttttttccaa atacatatca tcatttccaa
    33481 atgccgctgc tgcttcttgc tgagctgaag aaaaatgctg tgggagctct tcttttgatt
    33541 gaactttccg aatgcctttc ccgccaccac ctgcagctgc tttcagcatc actggataac
    33601 caattctgtc tgctatttcc aatgcttctt ctactgtaga taatacacca gtacttcctg
    33661 gaatgactgg gacatttgct tcttgcatca attgacgcgc attgatctta tttcccattg
    33721 catcgatcgt tgcactttta ggaccaataa atgtgatatt gcactcttca cacatggagg
    33781 caaatcggct attttcagat aaaaaaccaa atcctggatg aattgcttcg gcttttgtca
    33841 cgattgcagc gctcagtact tcctgaacat tcagatatga atcaacggct tttgccgggc
    33901 caatgcagat tgcttcatct gctaactgtg tatgaagtgc ttcggcatct gcttctgaat
    33961 atatcgctac ggtttggatc cctagttcgc ggcatgcacg aatgatacgt acagcgattt
    34021 caccgcggtt agctatcaat actttagaaa acatatttca cctatccaat cataaaggtt
    34081 aattccgctt cggctacttt tttaccgtct actttagcga ttcctttgcc tattccggct
    34141 gaagctttca ctttcaatat ctcaacttca aggtataagg tatctcccgg tgtaactttc
    34201 ttacggaatt ttgctttgtc caatccgccg aaataagccg tctttccctt gaattgttca
    34261 agagaaagta atgcaactgc tcctgcttga gccattgctt caacaatcaa tacccctggc
    34321 ataacaggtt cttctggaaa atgtccttga aaaaagggct cattgatcgt tacatttttc
    34381 ttagcaacga tccgttctcc ttcaaccatt tcttccactc gatcaatcaa aagcattgga
    34441 taacgatgtg gaatgatttc ttttatttct tgtatgttca ttgttttccc tctttcctgc
    34501 tttgcttatt tttgctgaat cctaaagagt ggctgtccat attcgacaac tgactcgttt
    34561 tctacaagga tttctgtgat cactccgtcg gcctcactag ttatctcatt catcagtttc
    34621 atcgcttcaa taatgcagat aacttctcct ttggcaactt gatcacctac ttttttgaaa
    34681 gctggtttgt cgggcgctgg ttgcagataa acgattccca cgattggcga aacaacctcg
    34741 attccatcaa atactgccga ttctttttct ttcattattt ctggagcagt tgttgcttgt
    34801 tcaggtatga cattgactgg ctccgaaact ggcaacgttt cgtttgtttg aaccatattc
    34861 atgcctcgac ttgttttatt tttattcata tacaattcaa attgaccttc acgcaaatcg
    34921 aattccgtta gcgaggattg atcaaattga ctgactaatt ctctgatttc attgatatcc
    34981 aaaattattc ctcccagcgt ttcatgcaca gaaccgcatt atgtccgcca aaccctaatg
    35041 aattactcat tgcataagta aaacttgcag gtttgctttc gtttttgaca actgtcaagt
    35101 caatctcagg atcttgtttt tctacattga tcgtaggtgg gataaattga tgttctaatg
    35161 ccagcaaagt tgccaaggct tcgatcccgc cggctgctcc caataaatgt ccagtcatcg
    35221 atttcgtgct gctgacgtaa gtatctctga aattttcacc taatgcgatt tggattgctt
    35281 ttgcttctgc ctggtcattg gctggtgtac ttgttccgtg agcattgata tatccgactt
    35341 gttccggctg tacaccagct tcttccatcg caagcttcat tgctttagat gcaccagaac
    35401 catctggagt aggagctgtc atatggtaag cgtcacagtt tgcaccgtat ccaacgattt
    35461 ctccatagat tttggcaccg cgagcttgtg catgttctaa agattcaaga actagaacgc
    35521 ctgctccttc acccatcaca aaaccattcc gattttcgtc aaacggaatc gaaccacgat
    35581 taggatcagt tgattctgag agtgctgtta aagctgcaaa tccagcaatc cccatctcac
    35641 aaatcgttgc ttctgttcct ccagcaagaa tgacatcaga atagccatgt ttgatgttac
    35701 ggaatgcttc cccaattgaa ttcgttccgc tagcacaagc tgttacgata gctgtacaaa
    35761 taccttttgc acctacacgc aaagcaatat ttccagctgc catgttggca atcgccattg
    35821 gtacgaacat tggagaaact cgttttgcac ctttttcatg tagacgaata acttggtttt
    35881 cgattgttgg aagaccaccg atcccagatc cgatgatgac gccaaaccgg tcttgattca
    35941 ttttttcttt atctaaaccg ctcatctcca ttgcttctaa agcagcgtag attccataga
    36001 tcgaaaacat atccattcgt ttgctatctt ttttaataaa atatttatcg taagggaaat
    36061 ctttgacttc acccgctaag gtaatccctg tttctgaagc atcaaattta gtgatcggtg
    36121 cgatgccatt cgtcccctct tttaagctat tcaaaaagct gtctgcgtca tttccaatag
    36181 gtgaagtgac tccatagcca gtaattacca ctcgattcat ttgtttgctc tccttttctt
    36241 atccgtgcat gaccaatccg ccatcgacat tgatgacttg tccggtgata tatggatttt
    36301 gtgctaagaa aactgctgtt tgtgcaatat cttctacttg tccgaaacgc tgcatcggaa
    36361 tttgcttttc cgcttgttcc ttgactttgt ctgctaaaac ttccgtcata tcagttgtga
    36421 tgaatcctgg cgcaatggca ttacatgtaa tgcctctagt tgcagcttct cgtgccacag
    36481 attttgttaa tcctactacg ccagctttac tagctgcata atttgcttga ccaatatttc
    36541 caatcaaccc agatacacta gagagattaa tgatggcccc ttctcgctgc ttcatcattt
    36601 ttttcaatac gtgctgcgtc atattgaagg ttccaatcaa attgatatcc agacattttt
    36661 tgaaatcttc tgcatccatt cgcatcacaa gtttgtcatt tgtgattccg gcattgttta
    36721 ctaacacatg gatcgatcca agcttttctt ctgcttcttt gatcatttgt cccgctttct
    36781 cataatcaga gatgtcaccg gaaactccta cgcattttac accgaatgct tcgatttctt
    36841 ctatcttttc ttttggaatc tctccgcgac cattcaaaat gatatttgct ccagcttttg
    36901 caaaagctaa ggccatcgct ttgccgattc ctcttgtact acctgtgata aatacatttt
    36961 ttccttttac atccataacg ttcctcctta tagtattgct attgtttcat caaatgtctg
    37021 cttattctca acacgcagaa ctggtattgt tttatcgatt tttttcataa atccgcttaa
    37081 tacttttccg gggccgactt cgatgacttg ttcgatgccg attgttttta acttatggat
    37141 actctcatag aaacgaacag gttgcatgac ttgctgttca agtagtcccg cgattgtttc
    37201 tttcttcatt atttccgttg ttgtgttgct tatgatagga aaagaaggtt cagagaattg
    37261 aatctgactt aggtccttcg ccagtttttt tgctgccggt tctaaaatag ctgtatgaaa
    37321 aggcccgctg acatttagag ggatcattct tttgaaccct ttttctttta atagtaaaac
    37381 agcttcgtct actgcttttt cttcgccacc aatcacgatc tgctgaggtg tattataatt
    37441 tgcaggagat acgatgccgt acttgctagc ttcatgacag ctttcctcga tcgtttcaat
    37501 cggcgcattc atcactgcaa ccattttccc gcttccagct ggggcagctt ctgtcatata
    37561 agcgcctctt tttgctacta acgcaactgc ttcagtgaag gatagcgcat tactagctac
    37621 taatgccgaa tattctccta aactcagtcc taaagctgca tctgggatga ttccatgctc
    37681 cttcaataag cgataatacg cgatgctgac tgttagaata gctggctgtg tatattgcgt
    37741 ctgatccaaa cgttcatttt ctgtaaagca aagttcagcc atatcgtagc cgagaatctc
    37801 acttgcttca tcaaatgtct gctttacaac ggcttcttca tataaatctt ttcccatacc
    37861 ttgatattgt gcaccttggc cactaaataa gaatgctgtt ttcatgtttt tctccaatat
    37921 atccagaata tatgtttgat tcttcctatt caaaaaggcc gagacattcc tatttcagga
    37981 aatgtcccag cccccaacct aattttaatg attatgcagt ttgtttttct acgtaagtca
    38041 ctaaatcttg aaccgttttg attccgtctt ctgtttcgat tttcacgtca aattcatctt
    38101 caatttcatt gatgatttgg aataaatcca agctgtcagc ttctaagtct tcttggatgt
    38161 cagtagtcaa tttgatttct tcagtttctt ttcctaattc ttctgcgatg atttcttgaa
    38221 ttttttcgaa taccataata atatattcct ccgatattgt ttgcttttta tttttataat
    38281 ttaacagtct acaactccat gagaatactt ccccatgtta aacctccgcc atagcctgta
    38341 aatattactt tctgctgttt tcctaaggtg atctttccac tagaaactgc ttcatccaat
    38401 aagataggga tgcttgcagc agatgtattc ccatatttat ccatattggt caggaacttt
    38461 tcttgcggca tctttacttt tcgggcaatt ttctcaataa ttctgaggtt tgcctgatgc
    38521 aaaagcagat aatcaactgg tttgtctttc atgacttctt ggatattgtt tgcaacgtct
    38581 cgtacggcaa aatcaaaaat atctctgcca gtcatttgca aataatacga accttctgaa
    38641 tcttctgaat agaacggatt tttattttgc tggtagccag cggtcaatga gctacttctg
    38701 gtaccgtcag ctgctaattt ttctttcagc aattttttct gtggattcgc ttctaagaga
    38761 atcccacctg ctccatcgcc aaacaacaca gcagtcgtac gatcagacca atcgattagc
    38821 ttagatagtg tttcaccacc aatcaccagt cccttagttg agccgcagcg gatcattttt
    38881 tcagccatgc ttaacgcata aacaaatcct gagcacgcag cactgatatc aaaagcgata
    38941 gcatgagttg ctcccaattt cccttgtact tgtgctgcta cagatggtgt cgtgtaatcg
    39001 ggcgacattg ttgctactaa aataaaatcc aaatctttag ggtttagatt tttctgtttt
    39061 atcaattttt ccgccactaa aatgcataaa tcagaagtct cctgatttgt tacacatctt
    39121 cgttcacgaa tacccgtcct tgacgaaatc cattcatcag aggtatccat catcttggaa
    39181 agatcattat tagtaataat cttttcagga aggacgctgg ctgttgcagt aatcattcca
    39241 aattgttcca ttaaatatcc tcacttatag tcttgcaaga agtcgtgcaa gtttttcagt
    39301 gcatccagca aggcatgctg ttcctccgta gccattccgt ctaagatgtt cttgaccatt
    39361 tcacgatgaa aatgctgatg gacccgaaat agtaactttc cttttttcgt tagtcccaac
    39421 tttacaacgc ggcggtcatc ttcactgcgg atacgttcga catagccttt ttttactaac
    39481 ttgttaatag cagtggtcaa ggtcccaaca gtaattgata gttctttggc tacttctgaa
    39541 gttgtttttt tcttgtacat cccgattgcc tcgatcgtgt gcatctctgt gattgacaga
    39601 tctttgaatt gagatttttt cagctcagac tcttcgattg tcaaaatatc attgaagaca
    39661 ctaacaagat aatcatttac tgtttttaaa tctggttcca tgttccaccg tccactaatt
    39721 agtttgatta tcaaatcttt tgatgttcaa actatatctt aacttttttc aaaatgcaac
    39781 cttaaaagat cattttttta ctatatgagc tttttttaag attttactca tttaaaacaa
    39841 ctgataaatc agcatttata atttctttta ttagaaaaat catctatctc acatattttg
    39901 attatcaaag tattttctgt gaacagaact atagaattcc ctaaaacttg tccattattt
    39961 caaaatttgt tatagtcttt ttattcaaag cgtaaaagga gatcctatgt taaaagaaaa
    40021 aatgaaaact ctccccatgc tgccgggtgt ctatcttatg aaagaccgag atggtaaggt
    40081 catctatgtt ggtaaagcaa aacggctcaa aaatagagta agcagctatt ttcatcagaa
    40141 taaacagcat tcaaaaaaag tattacgtat gattcatcat ataacagatt ttgatttcgt
    40201 cgtagtagac acggaattag atgctttatt gctcgagtgt caattgatcc aacattaccg
    40261 tcctttttat aaccgacaaa tgaattattt cagtaattat aattatgtac atattaccaa
    40321 taagggattt gtattaacag atactcctac ggctcgaaca tacgggcctt ttcgtctcta
    40381 taaaaaaatg ccgtctattt tgcgtataat ggaagaaact tatcaaatgc cttggttgtc
    40441 tgaaattagt ttgttagctt tgcgagtcca gcttcctgac ctacaggaaa tgacttttga
    40501 acagaaaaaa aaggaactcc aagggctgtt tcaaggccga aataaaaagc tactgactta
    40561 cttgaaaaaa agacaacaac atttcatcta tcagttgaat ttcgaaaaag ccggtatgct
    40621 tcaaaaagat atcgaattag ttacttattt tattagacgg atccaggaac aaaagcagtt
    40681 tttaagaaca ccatcgctca ccttcagcat gccattagca gcagatgaga gtcagaaaaa
    40741 gcactacctc gtttgttatg gtcaacttgc agaaacaatg attacctctg gtgacgtttc
    40801 tcctgacttc tattatgaaa agaaagaagc tcatttatct ttgaaacgtc aattgtcaaa
    40861 agaagaaatc gatcctgtac aaatcttgat cagttaccgc aaaaaattag aaaaagaaca
    40921 gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag caactaaatt agttgcttct
    40981 cggcttcttt taggcatctt caacttcaaa tgtcaattat cctaaaattt attggattaa
    41041 gtcatagatt tccatcgcta cgatatcgat attatcaaat tcgtattgct gatgagtatc
    41101 tgtttcagtt aattcaaatg tatcggtcgc tttatcataa gttactgtac acttctctac
    41161 gccttctttt tcaaaacggc gaacttgcag ttcattatct gctgactcga ccatagcctc
    41221 cagtcgtttt atgattgcga ctaattgtga atgtgtcatc ggaggtcctc cttttcttat
    41281 ttctgttata ttgtatcaaa agcatagcct acttgcattg attttctcta aaaaaactcg
    41341 ttttttatga ttttttttct ggtatttttc cttcaggcca ccaaagtttg atcaaagctt
    41401 gggttccgtc attctcaaaa cctttatctg ccaactcttc atatagtttc aatgcttggg
    41461 tagttgctgg caatacaaga tccatttttt ctgcttcttc taatgcaatc tttaaatctt
    41521 tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc ttttaaaata cgaggagaat
    41581 aattgcttaa agaccagtta gctgcacttc cacctgaaag cgtctcaatc actttcttta
    41641 aatcaagtcc gtttttttgt gcatagacaa gcatttccgt cattcctgtc atcgtacctg
    41701 caatcatgat ctgattagcc atttttgtat gctgaccttt cccagcagaa ccatgcaatg
    41761 taaatgtcgt accaaattct ttgaataaag gcagtacttg atcataaacg gcttcttctc
    41821 caccgaccat gatcgtcagt gtaccattct ttgctcctaa gtcaccaccg gataccggtg
    41881 catctaacgc atctgcgcct ttttcttttg ctgtttttgc aattttttct gccaggcttg
    41941 gtgtacttgt agtcaaatca actaaaatat gtcctgagat atctgcagaa aaaatccccg
    42001 actgtccata gtagatttct tctacatctt tggggtaacc taccattgta aagagaatat
    42061 tactttgttc tgctaccgct tttggtgtat ctgcccagat agctccttct gttactaaag
    42121 gatcagcttt tgcttttgtc cgattataga cagctacttc gtgtcctgct tctagtagat
    42181 ggcgtgcgac agcacttccc atgacgcctg ttccgataaa accaagtttc attttttcga
    42241 cctcctaaaa atcaaaaatc cttgcttcta tgctagtata cgctagaaac aaggattttt
    42301 tcataaaaaa tgtttgaatt ttatctttta aatgaattta attttatttt gattcgttca
    42361 atcgagtctt cttcttcttt tcttcgcatc tcttcttcat gataaacaac catgtcttga
    42421 taaaattcga tcatagcttc gccgaaatgt gtagatgaaa tatcataaag tttagcttct
    42481 aaaacttttg gatctttagg aacttcactg gaaatataat caataagtgc tggtgcaaag
    42541 tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat cgaacaatcg gttcaaataa
    42601 gcatttcctt ccacgacaca aggtgttccg gctgccatcg cttctgtata agtaagccct
    42661 tgtgtttctg aagtagaagc actgacaaaa taatctgccg ctttataata aagtgctaca
    42721 tcattgttgt ccacttctcc tgtgaactgc acaaattctt ctaactgcag ttctgagatc
    42781 aattcctcta actcttctac ataaggtcct tttcctacga taactaatct ggcgttagga
    42841 taagcggaga tgacctgagg aaacccatta atcaaggctt ggatattttt ctcataagaa
    42901 atcctgctta aagaaagaat catcaaatgg ttttcctgaa gacctagtga ctcccttaat
    42961 ttgctgatat cctgctgagt gatatcagaa cgtttgaatt tttcaatgtc gataccagta
    43021 ggaatcacgc gaagcggtgc tattacacca tagctgcgta atgtatcaat aacacgttca
    43081 cttggacaaa ctacacctgt cgtatgattt gcaaataatc tagacaagta ttttacatgg
    43141 gattgcctga tcacttttcc tttaccgata taatgaaggt agtcttcata catcgtatgg
    43201 taagtatgga tcagaggtat tttcaacttc ttagctacca tcttcccaag tatccctgct
    43261 ccgaattctg tatgggtatg aataagatct agttctagtt ctttggcaac caaataagca
    43321 taccacatgc ctctaacaac aactcttcga tccttgaaag aaataaatgg tacacttggc
    43381 attcgaatca catctttttc caacttatcc gcattaggat cggtcgttgt aaaaatatag
    43441 acttcatgcc catgtctttc taattcttgt tttaatgttt tgatcgatgt agcaactccg
    43501 ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt tcatgttttt cgcctccaaa
    43561 ctagtccttg acctgattta ctttttttaa tttgctagag actttttcat tcagtacact
    43621 ttcgtataca tttttaagtt cttcaccgat tttattgata cttcgctctt ttgctgtttg
    43681 ataaccggct tgtgtcgtac tcggtagttc ttgattggta atctgttgga tcagtcggat
    43741 aaattcttcg tttgtcttcc ccatatagca gttcttgcta tcttctaacc agccattata
    43801 cactggaata tctcgaacaa gtacgttttg tttgcttgct aaagcttcta aaacaacgat
    43861 cccttctgtc tcttcataag aagggaaaaa gaatagatct gcacctgaat acgcaccttc
    43921 tatgatctct ccacgaatgt aaccaggaaa caggacattt tcaggatgat cttcttttac
    43981 aattttccgg atgttccgag gaatcgagta catgggaaca tgaccaaacc aaataaatgt
    44041 gtattcgggc atcttcttag caatttctac gaaatcaatg attcctttac gttcgaaaaa
    44101 caaaccgaca caaatgatta ctttttgatc cggagacagt ttgaaatatt ctctgaactt
    44161 ttgttctttc attggatcag ggtgatattt ctctaagtcg atcccattgg aaatagcttg
    44221 aatcggcaca cggataccat agctttccaa caactgtttt gaataaggtg ttggcgtgat
    44281 cagatagtcc gctttttggt acaatcctac cagatatttt tttacgatag gagaaagctg
    44341 gttcgatccg ataaaggaat ttcggaaatc ttcctctgta gaatgcgcat gatagacaac
    44401 tttttttcct tctcttctgg ctttattgac catattatga ctattgattc cgtatgtatt
    44461 gatgtgcagg atgtcataat cttctttttc atctagtgta taagagatac cgacttccgt
    44521 cagagcacgt ttttgatgat caagcgcacg cccgattcca gattttgcaa gcatcttttc
    44581 actttcaaaa tataataata ccttcacctt acgtccccct tcacttatat agtataacat
    44641 agcctacaaa ctgtcactca aacttaagcc ctatttttgt ctattcttac tgacaaaaac
    44701 gttttcttaa aaaagaacaa aaaa
//
LOCUS       DAFELO010000016        41267 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFELO010000016
VERSION     DAFELO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.016"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            214..498
                     /locus_tag="LP452_000975"
     CDS             214..498
                     /locus_tag="LP452_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL2013723.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="LP452_000976"
     CDS             complement(779..1198)
                     /locus_tag="LP452_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL2013724.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="LP452_000977"
     CDS             complement(1200..1784)
                     /locus_tag="LP452_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL2013725.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="LP452_000978"
     CDS             complement(1831..3306)
                     /locus_tag="LP452_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL2013726.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="LP452_000979"
     CDS             3487..3798
                     /locus_tag="LP452_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013727.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="LP452_000980"
     CDS             complement(3913..6249)
                     /locus_tag="LP452_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2013728.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="LP452_000981"
     CDS             6391..7059
                     /locus_tag="LP452_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2013729.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="LP452_000982"
     CDS             7062..8105
                     /locus_tag="LP452_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2013730.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="LP452_000983"
     CDS             complement(8342..9223)
                     /locus_tag="LP452_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2013731.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="LP452_000984"
     CDS             9382..10110
                     /locus_tag="LP452_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL2013732.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="LP452_000985"
     CDS             10266..10583
                     /locus_tag="LP452_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2013733.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="LP452_000986"
     CDS             10576..11184
                     /locus_tag="LP452_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL2013734.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="LP452_000987"
     CDS             11239..12030
                     /locus_tag="LP452_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL2013735.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="LP452_000988"
     CDS             12168..12656
                     /locus_tag="LP452_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL2013736.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="LP452_000989"
     CDS             12707..13579
                     /locus_tag="LP452_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL2013737.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="LP452_000990"
     CDS             13755..14117
                     /locus_tag="LP452_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2013738.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="LP452_000991"
     CDS             14126..14878
                     /locus_tag="LP452_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013739.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="LP452_000992"
     CDS             14892..16694
                     /locus_tag="LP452_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013740.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="LP452_000993"
     CDS             16971..18011
                     /locus_tag="LP452_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2013741.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="LP452_000994"
     CDS             complement(18063..20207)
                     /locus_tag="LP452_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL2013742.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="LP452_000995"
     CDS             complement(20564..21937)
                     /locus_tag="LP452_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2013743.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22331)
                     /gene="rbsD"
                     /locus_tag="LP452_000996"
                     /pseudo
     CDS             complement(21937..22331)
                     /gene="rbsD"
                     /locus_tag="LP452_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="LP452_000997"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="LP452_000997"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL2013744.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="LP452_000998"
     CDS             complement(23384..24397)
                     /locus_tag="LP452_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2013745.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="LP452_000999"
     CDS             complement(24478..25077)
                     /locus_tag="LP452_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2013746.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="LP452_001000"
     CDS             25263..25964
                     /locus_tag="LP452_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013747.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="LP452_001001"
     CDS             25974..28685
                     /locus_tag="LP452_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013748.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29136..30764
                     /gene="treC"
                     /locus_tag="LP452_001002"
     CDS             29136..30764
                     /gene="treC"
                     /locus_tag="LP452_001002"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL2013749.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGAKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30824..32005)
                     /locus_tag="LP452_001003"
     CDS             complement(30824..32005)
                     /locus_tag="LP452_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2013750.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32257..33780
                     /locus_tag="LP452_001004"
     CDS             32257..33780
                     /locus_tag="LP452_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL2013751.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33849..34577)
                     /locus_tag="LP452_001005"
     CDS             complement(33849..34577)
                     /locus_tag="LP452_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2013752.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34590..35870)
                     /locus_tag="LP452_001006"
     CDS             complement(34590..35870)
                     /locus_tag="LP452_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2013753.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36011..36613)
                     /locus_tag="LP452_001007"
     CDS             complement(36011..36613)
                     /locus_tag="LP452_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL2013754.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36858..37064
                     /locus_tag="LP452_001008"
     CDS             36858..37064
                     /locus_tag="LP452_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013755.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37376..38095
                     /locus_tag="LP452_001009"
     CDS             37376..38095
                     /locus_tag="LP452_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2013756.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38176..38859)
                     /locus_tag="LP452_001010"
     CDS             complement(38176..38859)
                     /locus_tag="LP452_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL2013757.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39077..41263
                     /locus_tag="LP452_001011"
     CDS             39077..41263
                     /locus_tag="LP452_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2013758.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13385 a         7323 c         8276 g        12283 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcatg
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctggtttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggaac gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa acaaacggca
    28741 aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
    28801 ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat actcaaataa
    28861 aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa agcttttgaa
    28921 caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat agacaaatac
    28981 atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata aagataaatt
    29041 ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata taagttgtgg
    29101 taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac aaagtcattt
    29161 accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga gatttaaaag
    29221 gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt tggctaaacc
    29281 ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac acagccatcg
    29341 atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca aagaaatacc
    29401 ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat gaatggttca
    29461 aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga gaagagccaa
    29521 cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat acggggaaat
    29581 attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac caagcagtaa
    29641 gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggcaaaa ggattccgtt
    29701 tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa gaaaatgatg
    29761 gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa ttgaaccaag
    29821 caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct acgacgatcg
    29881 aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc tttaatttcc
    29941 atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca tttgattttg
    30001 cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt aacggctgga
    30061 atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt gtagctatcg
    30121 atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg aaccgtggga
    30181 cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat tcatcgattt
    30241 ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa caaggattca
    30301 cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct cgaacaccca
    30361 tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg ttaaaagttg
    30421 gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat tctattttgt
    30481 cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca caaggagatt
    30541 atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa tttgaaggac
    30601 agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc atcccagaag
    30661 gattcacaga agccaatatt cttatcgata attattccga tactgttctt catcaaagtc
    30721 tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa aacacaaaac
    30781 gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta aaataatctt
    30841 cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta ttgtagcgtt
    30901 gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag ataggaatac
    30961 caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg attaagatcg
    31021 taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag tcaccgtgga
    31081 gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta gagtcttgga
    31141 atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga atgatacata
    31201 acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt aagaaacaaa
    31261 cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga aatgaaccca
    31321 attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct ggtgggacaa
    31381 tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa gcgacaattg
    31441 gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg ctgatccgtt
    31501 gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc acgtcgtcaa
    31561 tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa aattgtcgga
    31621 gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc tttgcctgtt
    31681 cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa ataatcacat
    31741 aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg atgactggtt
    31801 ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta gcgataatga
    31861 ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc agcgcaagta
    31921 agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg atcaaacgat
    31981 tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact tcattataga
    32041 caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg tcaatcattc
    32101 atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt aaccatatga
    32161 taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg aatcggaaca
    32221 ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat aggtatattg
    32281 gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa agccgaggac
    32341 agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt tacaaaagca
    32401 atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga ttcccacgat
    32461 tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt tgtttataat
    32521 gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga aggcaatgtc
    32581 catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga aggacatgat
    32641 catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca tgtatggcta
    32701 gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat tgaagcttac
    32761 ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa acttcaagca
    32821 ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt tgtcacgcaa
    32881 catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc gattgctggt
    32941 ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca ctttgttaaa
    33001 gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat tgccagaacg
    33061 ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg attgacgaat
    33121 gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct cgaagcgttg
    33181 aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa aaaagaaaag
    33241 agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct ttcagattgg
    33301 gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga tcctgttttt
    33361 gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga atattatacg
    33421 aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat ggcatttacg
    33481 tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga aatcttgacc
    33541 tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa ggaaagtggc
    33601 gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc cgaacatttt
    33661 catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga gaattggcca
    33721 acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct tttgcactga
    33781 taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc
    33841 ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg
    33901 tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata
    33961 acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc
    34021 tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc
    34081 gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct
    34141 attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg
    34201 atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt
    34261 tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt
    34321 tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg
    34381 tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca
    34441 ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc
    34501 atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa
    34561 atataaatgg gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa
    34621 aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca
    34681 tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt
    34741 tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg
    34801 gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct
    34861 tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta
    34921 tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc
    34981 atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt
    35041 agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa
    35101 gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt
    35161 tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa
    35221 tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt
    35281 ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta
    35341 ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag
    35401 tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac
    35461 caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag
    35521 acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa
    35581 aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc
    35641 gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct
    35701 atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa
    35761 gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag
    35821 atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc
    35881 tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta atttttcagg
    35941 gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt
    36001 agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt
    36061 ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa
    36121 agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg ctgatcgcct
    36181 tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc
    36241 catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga
    36301 tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca
    36361 gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat
    36421 aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa
    36481 cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt acttggacat
    36541 acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa
    36601 ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa
    36661 cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa
    36721 tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt
    36781 tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag
    36841 gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat
    36901 ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata
    36961 gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc
    37021 actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt
    37081 gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa
    37141 aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata
    37201 ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat
    37261 aggagttact tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc
    37321 ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc
    37381 gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat
    37441 gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt
    37501 gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa
    37561 caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc
    37621 catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt
    37681 agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc
    37741 gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc
    37801 gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc
    37861 ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc
    37921 caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca
    37981 aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc
    38041 gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta aataatataa
    38101 aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt
    38161 tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa
    38221 tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc
    38281 aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact
    38341 tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa
    38401 gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt
    38461 gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa
    38521 tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata
    38581 tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt
    38641 tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag
    38701 ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc
    38761 aaaagcacca tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa
    38821 aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga
    38881 atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa
    38941 actaaaatca tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa
    39001 atagacaacg aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg
    39061 gaaggaaatc aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg
    39121 ctgggagaac aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc
    39181 tacctatgaa gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa
    39241 agaaagtgaa ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt
    39301 caatcaagaa caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc
    39361 aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca
    39421 aaaacaagta gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga
    39481 actaaaaaaa cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt
    39541 ccatagtatc ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat
    39601 gcaaggaata caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca
    39661 acagctgata cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc
    39721 ttctcggtta gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt
    39781 taaagaaaaa tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta
    39841 tggattgctc acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga
    39901 tgtactccaa aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca
    39961 aaaatatgat tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat
    40021 cgtgaaacgg atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca
    40081 gagtatctat ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata
    40141 tcctaccaaa actcttttct tattaactaa ttatcgttct cgggcagcta ttgtccaagg
    40201 agcaaataga ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc
    40261 acaaaaagaa gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca
    40321 gtggctaatc aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat
    40381 catattatac cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa
    40441 tattcctttt gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt
    40501 ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa
    40561 tcaggcctac cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga
    40621 atgcgatcaa tacttttctc ctaaatatca aaatatatcc gttgcagatt atattttgtc
    40681 gataagcccc aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg
    40741 taaaacagac cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg
    40801 tatagctaag gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca
    40861 tgctactttc agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt
    40921 aaaaacccgc ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat
    40981 tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag
    41041 tttgccttat aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga
    41101 accagtaacg acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag
    41161 aaatcgtctg tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag
    41221 atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag tgataga
//
LOCUS       DAFELO010000017        41152 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFELO010000017
VERSION     DAFELO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.017"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            239..481
                     /locus_tag="LP452_001012"
     CDS             239..481
                     /locus_tag="LP452_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL2013759.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            478..948
                     /locus_tag="LP452_001013"
     CDS             478..948
                     /locus_tag="LP452_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2013760.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1076..2290
                     /locus_tag="LP452_001014"
     CDS             1076..2290
                     /locus_tag="LP452_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL2013761.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3279..3488
                     /locus_tag="LP452_001015"
     CDS             3279..3488
                     /locus_tag="LP452_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL2013762.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3600..5213
                     /locus_tag="LP452_001016"
     CDS             3600..5213
                     /locus_tag="LP452_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2013763.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5248..6459
                     /locus_tag="LP452_001017"
                     /pseudo
     CDS             5248..6459
                     /locus_tag="LP452_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6482..7408
                     /gene="glsA"
                     /locus_tag="LP452_001018"
     CDS             6482..7408
                     /gene="glsA"
                     /locus_tag="LP452_001018"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL2013764.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7616..9076
                     /locus_tag="LP452_001019"
     CDS             7616..9076
                     /locus_tag="LP452_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013765.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9297..11060
                     /locus_tag="LP452_001020"
     CDS             9297..11060
                     /locus_tag="LP452_001020"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL2013766.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11144..11602
                     /locus_tag="LP452_001021"
     CDS             11144..11602
                     /locus_tag="LP452_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL2013767.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11711..12394
                     /locus_tag="LP452_001022"
     CDS             11711..12394
                     /locus_tag="LP452_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL2013768.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12541..13155
                     /locus_tag="LP452_001023"
     CDS             12541..13155
                     /locus_tag="LP452_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL2013769.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13171..13377
                     /locus_tag="LP452_001024"
     CDS             13171..13377
                     /locus_tag="LP452_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013770.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13918..15048
                     /locus_tag="LP452_001025"
     CDS             13918..15048
                     /locus_tag="LP452_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2013771.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15140..15895
                     /locus_tag="LP452_001026"
     CDS             15140..15895
                     /locus_tag="LP452_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2013772.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15953..17992
                     /locus_tag="LP452_001027"
     CDS             15953..17992
                     /locus_tag="LP452_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL2013773.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18101..19252)
                     /locus_tag="LP452_001028"
     CDS             complement(18101..19252)
                     /locus_tag="LP452_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL2013774.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19406..19936)
                     /locus_tag="LP452_001029"
     CDS             complement(19406..19936)
                     /locus_tag="LP452_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL2013775.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20035..20385)
                     /locus_tag="LP452_001030"
     CDS             complement(20035..20385)
                     /locus_tag="LP452_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL2013776.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20542..22071)
                     /gene="ahpF"
                     /locus_tag="LP452_001031"
     CDS             complement(20542..22071)
                     /gene="ahpF"
                     /locus_tag="LP452_001031"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL2013777.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22081..22644)
                     /gene="ahpC"
                     /locus_tag="LP452_001032"
     CDS             complement(22081..22644)
                     /gene="ahpC"
                     /locus_tag="LP452_001032"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL2013778.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22788..23450)
                     /locus_tag="LP452_001033"
     CDS             complement(22788..23450)
                     /locus_tag="LP452_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2013779.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23634..25280
                     /locus_tag="LP452_001034"
     CDS             23634..25280
                     /locus_tag="LP452_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL2013780.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25271..26353
                     /gene="uxuA"
                     /locus_tag="LP452_001035"
     CDS             25271..26353
                     /gene="uxuA"
                     /locus_tag="LP452_001035"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2013781.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26461..28014)
                     /locus_tag="LP452_001036"
                     /pseudo
     CDS             complement(26461..28014)
                     /locus_tag="LP452_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            28162..29303
                     /locus_tag="LP452_001037"
                     /pseudo
     CDS             28162..29303
                     /locus_tag="LP452_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29429..30508)
                     /locus_tag="LP452_001038"
     CDS             complement(29429..30508)
                     /locus_tag="LP452_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2013782.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30611..31051)
                     /locus_tag="LP452_001039"
     CDS             complement(30611..31051)
                     /locus_tag="LP452_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2013783.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31108..31517)
                     /locus_tag="LP452_001040"
                     /pseudo
     CDS             complement(31108..31517)
                     /locus_tag="LP452_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
     gene            complement(31697..32131)
                     /locus_tag="LP452_001041"
     CDS             complement(31697..32131)
                     /locus_tag="LP452_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2013784.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32231..33079)
                     /locus_tag="LP452_001042"
     CDS             complement(32231..33079)
                     /locus_tag="LP452_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2013785.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33468..35234
                     /locus_tag="LP452_001043"
     CDS             33468..35234
                     /locus_tag="LP452_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL2013786.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35336..36595
                     /locus_tag="LP452_001044"
     CDS             35336..36595
                     /locus_tag="LP452_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2013787.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36742..38043
                     /locus_tag="LP452_001045"
     CDS             36742..38043
                     /locus_tag="LP452_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013788.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38046..38897
                     /locus_tag="LP452_001046"
     CDS             38046..38897
                     /locus_tag="LP452_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013789.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38910..39725
                     /locus_tag="LP452_001047"
     CDS             38910..39725
                     /locus_tag="LP452_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL2013790.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39876..41042
                     /locus_tag="LP452_001048"
     CDS             39876..41042
                     /locus_tag="LP452_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2013791.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT        13972 a         7121 c         7991 g        12068 t
ORIGIN      
        1 aaaataagaa attgattgtc gttttctttt gcagaaattt aagtgcaggc aattatggta
       61 gttgtcgtaa ttgctgtcag ctcagtgttt atcactgatg gtgtgaaaga tttacggttg
      121 gagatgttca ataagattat tggtgcttaa ttatttttat tgtagtgctg atatgagaat
      181 gttataattg gggtataaaa catacaaata aatttgctga aaaatgaggt atcaaaatat
      241 ggaaaatgaa ttactggaaa acttaggaag acttcacaca actgaacttg gcgttattag
      301 aattaaaaag aacttatctt tgaatgtaga aaatgtaatt gaatggtgca aagaaaaaat
      361 tagcctttct aatgcggaaa tcacaagaaa aggaaaaaac tggtatataa cgattgataa
      421 ttgtattata acggtaaatg cgtacagtta taccgttatc actgctcaca aggtaaaatg
      481 ataaattttt ctataaagga attaaacgca tttgcagtta ttgggcagga agtagaactg
      541 actaattatc aaaaaaagaa tattcagatt agtacacagt tttggagaaa gtttaacagt
      601 agtttgaaaa aagcgtatct ttcacaatca ggaaattggg taaaatatgc ttttatggaa
      661 agaagaaatg gaaaacttta ttatttctgt tctattcccc aaagaaccat taccccagag
      721 ggctttctgt ataaagaaat accgtcttat aaatatttgg ttgtggagca tattggtgct
      781 atggataaaa tatatgaaac ttatggcaag atttatgaag aaattattcc tagtacatcg
      841 tatattccta taaaagatat tatcctacac tttgaaaaat acgattatcg ttttcattgg
      901 aatagtgata attcagttat tgaaatttgg atacctattc aagattagaa gcaattttat
      961 atccatacac acaaagcagt tagtcttagg attgactgct ttttctatac agatttttaa
     1021 tgacaggcaa ttatcaatta tggtagttgc cttttttgat tggaggaatt gaagaatgaa
     1081 tgataaaaac tttattgaag aactaagaca aaagcgtgag gaatacggag taacacaaac
     1141 aaggcttgct gttgcctgtg gtatcagccg tgaatattac aaccgcattg aaaaaggaaa
     1201 acagccattg aatgacgaat taagagaaat catagaaaaa cagattgagc gtttcaatcc
     1261 gcaagaacca ctattcttat tgattgatta ctttcgtgtc cgctttccta ctacggacgc
     1321 attggcgatt atccgtgatg tattacaact gaaatctaat tacatgcttt atgaagatta
     1381 tggaaaatac ggatatgaaa gcaaatatgt acttggcgac atcaatatca tgtgttccat
     1441 gcaggagcat ttaggtgttt tattggaact gaaaggcaaa ggctgtcggc aaatggaatg
     1501 ttatctgctg gcacaggaac gctcatggta tgacttcatg ctggattgta tgacggctgg
     1561 tggtgtgatg aaacggcttg atttggctat caatgataaa gcaggaatat tggatattcc
     1621 aaagttaaag gaaaaataca aggctggcga atgtatgtcc tacttccgta tgcagaaaga
     1681 ttacagcggt acagaaaaat gcggtagcga tttaccaaag aatacaggag aaaccttata
     1741 tctcggttca acaagtagcg aattatatat gtgtgcttat cagaaaaatt atgagcagta
     1801 tgtcaagaat ggcacagaaa ttgaagatac ggagattaag aaccgttttg aaatacgcat
     1861 gaagaacgaa cgagcctatt atgcggttgt agatttactg acctatcggg acgctgaacg
     1921 caccgctttt tctatcatca atcattatgt ccgctttgtt gacagagagg acgacaagcc
     1981 aaaaagtcaa tggataacaa atgatgattg ggcatggttt gtaggggaaa acagagagcc
     2041 gatacgctta accacaaagc cagagcctta cactttacaa aaagcgttgc attggctaca
     2101 aagacaagtc gcaccaacca taaaaatggt acaggcatta gacagagaaa atcgtacaac
     2161 gatactgaaa gatatgattg agcaggcaga actaaaagac aagcataaac atttattaca
     2221 attagagaaa tcaaccatag aagaacgtat agataccgct gttccacaag agaatgacgg
     2281 tattttttaa tgtaattgaa aaatttcaga tttagtcagc aaagagctgt tttcattccc
     2341 tatgtgaagt gaaagaagaa aaataaattt tttattaaat ctgcaacaaa atggctactt
     2401 gcgtacagat atggtgcgag aaaaggaaat ctaaactttt tttgaaaata ggtcattaaa
     2461 tcggggcttg ctgtccctat atgaagtgaa agaagaaaaa agttttgaaa aaatgacgga
     2521 aattcgtgat tagtggacag tcttctgtga aaagaggaaa aacatcatca ttttgtattc
     2581 aaacaggcat tagcagttga gtgttgatgt gaaaagatga aaatactttg ttctcaactt
     2641 aacaatttaa ggtttccgtt ccctatatgg tgcaaagaaa atatttcatt ctattgttgg
     2701 cagtaacggt atggtttggg tagttagggt gtgaaaagga aaatattctt ttttcatcat
     2761 caacttagca aaatgacatt ttctatccct atatggtgca tgaaaagaag ttaaaatttt
     2821 tccatttaaa cttagcaaat ggacgtttgc tgtacagata gtagtgaaaa aggagaaaat
     2881 caaccgttga ttgccaaaat gaaccgttcc atttgtacta ataatggaaa aagttttgga
     2941 ttttggttac gtttctcctc ttttccaacg cgatatatag gaaagacgat ttttttcttt
     3001 caatcgttct ttgacaactg aataaagcta ttcaatacgt ttgacagaca gctagccgtt
     3061 gaaacaaata cgccacgacc tttcccctgc aagagcgagc gaaaacctat ttgtgttctt
     3121 gtaaaagatt tgtcctcttt tatcgccatg acctgtactg tcagataatg atactcccgt
     3181 acagccacag cttgagcatt aagagcgtcg cacgcaatgg gtacggctac ataagaacta
     3241 tgcagaggtg gaactcctgt gggctatgac aaaagccgtt gaattgctta gaaagatttt
     3301 acagaaaggg ggtatgtgtt attgataatg ctgtaaaaac aattcaaaag aaaaatggca
     3361 agtgtggcat tggcaacaga ggaaagacaa agattgtagt aaaagagcat ttttccgaaa
     3421 agggcaaaac aatggaagaa cttctaactg atgtcatgct ggaaaaagca aagcagacaa
     3481 tcgcataaaa tcggtgcagt tgtaagaaat agattgaatg acaaaaagta cccgtgttat
     3541 actttacttg caagcaggta ttgtaaacac gggttagtta taaaggagga taactgacaa
     3601 tgaaacaaca gatttacaat actgcacttt atcttaggtt aagccgagat gatgaattac
     3661 agggcgaaag ttccagcatt accacacaga gaagtatgtt gcgtttatat gcaaaagaac
     3721 atcatttgaa tgtcattgat gaatatattg atgacggctg gtcgggaaca aattttgaca
     3781 gaccgagttt tcaaagaatg attgaggata tagaggcagg aaaaatcaac tgtgttgtaa
     3841 cgaaagacct ttcccgtctt ggcagaaatt atattatgac aggacaatat acagaattgt
     3901 atttccctag tcataatgtc cgctacatag cgattgatga cggtgtagac agcgaaaaag
     3961 gcgaaagtga gattgcacca tttaagaaca tcatcaatga atgggtggca agagatacaa
     4021 gccgtaaagt caaatcagca tttaagacaa agtttgcgga gggggctcat tacggtgctt
     4081 atgctccgtt aggatataag aaacaccctg acatcaaagg aaaactgttg gttgatgatg
     4141 aaacaaaatg gattattgaa aaaatctttt ccctagccta tcaaggttac ggaagtgcaa
     4201 aaatcacaaa gcagttaaga gcagaaaaag ttccgacagc gtcatggcta aattttacaa
     4261 gatacggtac ttttgcacat atctttgagg gaaaacccga aagtaagcgt tatgagtgga
     4321 cgattgctca tgtcaaggcg atattgaaaa gtgaggttta tatcggaaac agtgttcaca
     4381 atatgcagtc tacggtatcg ttcaagagca aaaagaaagt gcgtaagccc gaaagtgaat
     4441 ggtttcgagt agaaaacact cacgagccga ttattgacaa ggaagtgttc tatcgtgtgc
     4501 aggagcagat aaaatcaaga cgcagacaga caaaggaaaa ggcaacgccg atttttgcag
     4561 ggcttgtcaa gtgtgcggat tgtggttggt ctatgagatt tggaacgaat aagacaaata
     4621 aaacgcctta cagttattat gcttgcagtt actacgggca gtttggcaaa ggtaattgtt
     4681 ctatgcacta cattcgttat gatgtgctgt atcaagccgt attggaacgc ttgcagtatt
     4741 gggctaaggc agtacagcag gacgaagaaa aggtattgaa caagatacag aaagctggca
     4801 atgcagagag aatacgggaa aagaagaaaa aggcaagtac attgaagaaa gccgagaaca
     4861 gacaaaatga gattgaccgt ttatttgcga aaatgtatga agatagagcc tgtgagaaga
     4921 taacagagcg aaattttgtg atgttgtcca gcaagtacca aaaagaacag atagaactgg
     4981 aacagcagat aacaagccta agagaagaac taagtaaaat ggaacaggat atgataggtg
     5041 ctgaaaagtg gattgagtta atcaaggaat attccgtacc aaaggaactg acagcaccat
     5101 tattaaatgc catgatagaa aaaatcctca tacatgaagc aacaacgaat gaggataacg
     5161 aaagaataca ggaaattgag atatattacc gatttattgg aagagttgag taatcaacaa
     5221 gagtaatatt tttaactaag ggaaaccggc ggtgtctata cttgggtcag tcgcacattg
     5281 ggaaaacgat tcggttttgc cgcaatcttt ttccaatggt ttgagataac agttggctac
     5341 ttgaccatgc tgtatttctt aactggtgca ttatcctatg caacgggtat tggtgctata
     5401 caaaacaata aatatctgaa attggcaatc ctattgatta ttttctgggc tattttactc
     5461 tcacagttaa aaggaaccaa atacacttca ttgattgctc ggatcggttt tgtagctggt
     5521 atcttgttgc cagcattagt cttgtttggc ttagggatcc attatgtcgc ttcaggcgct
     5581 ccactacaaa cgatactgtc tatgaaaaca ttgattcctg atttttcgaa attgcctacg
     5641 ctggttgttt ttgtttcctt tattttagca tatatgggcg tagaaacatc tgccagtcat
     5701 gcaaatgaaa tggaaaaccc taaaaaggat tatccattag caatgtttgt attggttatt
     5761 ctggcaatca ttttagatac ttttggcgga ttgaccgtcg ctactacagt acctcaacat
     5821 ggtttgtctc ttaatacagg ggtgattcaa tcgttagaat tcctattaag acacattgat
     5881 ccaaatcttg catggattgc gaaaattctt gcgattcttg tttgtttagg tgtcatcgga
     5941 gaaattgctt cttgggtaac ctctccttca aaagcacttc acgtagcagc agaagaagga
     6001 ttacttcctg aatattttgc aaaagaaaac acacacggtg ttcctgctca tttgatgatc
     6061 gcaaatggga tcgttgcaac aatctgggca gccgtcttta cattaagcgg cggggggaat
     6121 aatctttcat ttcttgcagc gatgtcattg actgttgtaa tctacctgat gttgtacttt
     6181 atgtttttcg cgggctattt gaagctggtt tttaaagatg acagtcttga aagagcctat
     6241 caggttccag gcggaaaagt tgtaaaagta atcgttgctt gtttaggtct tttgacttca
     6301 ctttttgctt tttgtatctc atttgttcca cctgctagta tcgcagctgg acagcatggg
     6361 atttatgaaa cgattctgat tgtttcttat cttatcgtct tagttattcc atttacgatc
     6421 tacgcatgcc gtcataaatt ccagcatgca gaaaaataaa aaatgaaagc tggttattca
     6481 aatggaagta aaagaattag aacaattgat tggaaaaaat ttccactatt atcaaacagg
     6541 acaagtggcg gattacattc cagcattagc tgaagtggat cccaaacaat taggtatggc
     6601 gatttatgat ttggatacaa aacaactgat tgagggagga gacgccgatg tgcgctttgc
     6661 catcgaaagc atgtcaaaag ttcccgtatt gttattagca atcaaagaca acggaataga
     6721 aaaagtcttt caaacaatca acacggaacc aaccggtttt gccttcaatt ctatcatgaa
     6781 tatggaaatc aatcatcgca aacatccaat gaatcctttt gttaatgcag gagcgattgc
     6841 aacaacctcg ttgattgccg gtaaagatgc agatgaaaaa tttgagcgaa tcttgtcatt
     6901 tatgaaagag atctgtgatg atcctaatat cacattgaat gaagaaatct atcattctga
     6961 atcaagaaca ggtgatatca atcgctctct tgcttactat atgaaaggta atcaaatgat
     7021 cgaaggagac gtaccggaga tactcgatgc ttatttcaga caatgctccc tcaatgtcac
     7081 cgctacaggc atagcaaaat tagctgctgt gctggcaaat aaaggaattg ctccttggaa
     7141 tggcaaacaa atcattactg aagaaagtgc tacaatcgtc aaatcaatta tgacaactgc
     7201 tggtttgtat gatgaatcag gagagttttc cgtccatgtt ggtgtacctg caaaaagcgg
     7261 tgtaggcggc gggttgatgg cagctgtgcc aaatcgttac ggcataggtg tcttcagccc
     7321 ggcactagat ccatttggta acagcgcagc tggtatccaa ttgttgaaag atgtagtcaa
     7381 agaactagac gctgacatct ttgaataacc attcttgaaa gaggctgggg taaaagtcca
     7441 aacggctcaa agctacctac aatgaggctg aaattgtgag cttgtttgac ttttgtccca
     7501 gtttcttttt ttattggttt ttgaaagggc ttttctaaat aaaaaggaaa caaaaatacg
     7561 atttacagat atgattaaat aaaatcataa gaaaaactgg aggcgtgtat caggaatgct
     7621 ggaaaattac atcgagcgaa atatatttag aaaagtgtat ctgtgtgaac aattatttga
     7681 attccaagaa atagacatcg aacaaacagc tatttctcta agggtgacta caccaacgat
     7741 ccttcacgat ctagaaagtc tagccgagtg tctagaatat tgtataaaag aacaagtaag
     7801 agagaagcat aagtataaac tcgtattcaa acatggtatt gctctttctg agctcacaca
     7861 gtttctgtac ggtcaatctt attttttaaa gtttttaagt tattatttta atggtatatt
     7921 cacgagtaca gaactggctg atcttgaatt tatttctttg agtaaagtat atacaatcaa
     7981 aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat
     8041 cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg
     8101 ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga
     8161 gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgagaaat
     8221 agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag
     8281 cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat
     8341 tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta
     8401 taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt
     8461 tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga
     8521 cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc
     8581 agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct
     8641 gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag
     8701 atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca
     8761 agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt
     8821 ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt
     8881 atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt
     8941 gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat
     9001 ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa
     9061 aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc
     9121 tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt
     9181 ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata
     9241 aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga
     9301 gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg
     9361 ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg
     9421 atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt
     9481 atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc
     9541 ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg
     9601 gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat
     9661 ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg
     9721 tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca
     9781 atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg
     9841 atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa
     9901 gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg
     9961 atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac
    10021 ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat
    10081 atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag
    10141 aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc
    10201 ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa
    10261 aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt
    10321 ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac
    10381 gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg attattttag
    10441 tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt
    10501 acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct
    10561 ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac
    10621 atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag
    10681 taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag
    10741 aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga
    10801 tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca
    10861 cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg
    10921 attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg
    10981 tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc
    11041 gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta
    11101 tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga
    11161 agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt
    11221 cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa
    11281 gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc
    11341 aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa
    11401 aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga
    11461 aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca
    11521 tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga
    11581 atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc
    11641 tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata acattactgg
    11701 aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc
    11761 tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa
    11821 agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg
    11881 aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt
    11941 tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc
    12001 actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat
    12061 gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca
    12121 agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc
    12181 acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat
    12241 cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt
    12301 aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag
    12361 atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga
    12421 caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta
    12481 cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc
    12541 atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt
    12601 tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat
    12661 atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg
    12721 atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa
    12781 gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat
    12841 ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc
    12901 atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat
    12961 gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa
    13021 tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg
    13081 aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt
    13141 gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca
    13201 gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac
    13261 ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag
    13321 gaaatagaaa tgccagatca aacgattagc ggattgttta attactttaa caaataaata
    13381 tttattccga aaagtgaaaa aacatataaa aaaacacata aaactaaaaa attatcaaag
    13441 aatcctggtg caactgaaag aatttgcaac gggattcttt caacttattt gttatttttt
    13501 tctaaaaaat attaaagatt gttaaatttc actatttcat ttcaaattcc attgcctaat
    13561 cattaggaaa agtctatcat gaactcgtaa atagaatata gtaataacct tgttaggtga
    13621 ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac
    13681 agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta cgttgtatag
    13741 tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg
    13801 accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc tcctcacctg
    13861 acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg gtggattttg
    13921 ggtccttatt tgactttgtc catgatcggt cttttagaag tatacagtgc cagttcttat
    13981 cgtttattgc aagccgatga aaatacaaaa agtctattat tgcgtcaact aattttcata
    14041 tttttgagtt ggagcgtgat cttcttagct cgttcagtca aactacacta tttacttcac
    14101 cctaaaatag caggatacgg tttagcctta tcgattttct ttttagtatt agtaagaata
    14161 gggatattcg gtgtcactgt caacggcgca caacgttgga tctctctgtt tggcattcaa
    14221 ttccagcctt ctgaactggc aaatcttttt ttgatttttt atttaagctg gttttttcgt
    14281 gacggaaata gtagcccaaa agatctaaaa aaaccattcc tgattacagt aggtataact
    14341 tttctgattt tatttcagcc aaagattgct ggagcattga tgatcctttc gattgcgtgg
    14401 gtcatatttt gggcagcggc ggttccattt aaaaaaggga tctatctaat cgttactttt
    14461 tctgcattgc tgattggagc agcaggcggg gtattatatt taggaaataa aggttggctt
    14521 ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt taagagattc ttttatcgat
    14581 agtcatggtg cgggctatca aatgacccat tcgttttatg cactatataa tggtggcata
    14641 tttgggcggg gattaggaaa tagtatcaca aaaaaagggt atcttccaga gacagaaaca
    14701 gattttattt tttcgattat tacagaagag cttgggctaa tcggtgcatt gtgtgtgctt
    14761 ttcttattgt tctcattatg catgagaatc ttttgtttaa gcagtcgctg taaaaatcag
    14821 caagctggat tgtttctttt aggattcgga actttgctat tcgttcagac gataatgaac
    14881 gtgggaagta tcgcggggct gatgccaatg actggtgtgc cattgccatt tgttagctac
    14941 ggaggaacaa gttatctgat cctttcttta ggaatcggaa tcacgttgaa tatttcttca
    15001 aagatacagg ccgaagagtt ggcgctctat cgacctgaaa aacaataaag agaaactgag
    15061 aaactctcag tttgaattat cataaagtac aatcttttat ttttcaagcg agaggttctt
    15121 ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc atattatcta ttttgttact
    15181 gcttacatta gtagttggct atttttcgat tgagtttatc catggatttt cgtctgcaaa
    15241 acagagttca actgtgaaaa aggtagagcc gaaaagtatc cctaccacac taaatgtggc
    15301 tttgattggt tcagatgctc gttcgaaaga cgaaaacggt cgctcggatt cacttctggt
    15361 tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga gagattcata
    15421 tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt atgggggagt
    15481 tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct attatgcaag
    15541 tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg gagtcaagat
    15601 cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag agcaagtgat
    15661 ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag gagacttcgg
    15721 gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga aagacgtaac
    15781 ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc aaacgaacct
    15841 tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat gttagaataa
    15901 acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa aaatgaaaag
    15961 aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag tgatcttaat
    16021 agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc aagcagtaga
    16081 agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag attataacaa
    16141 agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg aaaaagagat
    16201 cttagataaa taccagaata tatacggtgc tgctgatgtc aaaggacttc agatatcgaa
    16261 tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag caaagatgaa
    16321 tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca gaaatgatgg
    16381 tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag gaaatgacaa
    16441 agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa atggtgaacc
    16501 attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac ttggagatgg
    16561 aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa cagaagatgc
    16621 tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc cattgaaaat
    16681 cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag tagacggcag
    16741 atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag atatcacagc
    16801 agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc gctcaggttt
    16861 ggagatggct tttgataagg atcttcgtgg gacaacaggt gggaaattaa gcattacaga
    16921 tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg gcaaagatat
    16981 caaattgaca atcgatgcga aggcacaaaa aacagctttt gacagtctag gaggaaaagc
    17041 tggatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg ctagctctcc
    17101 aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca aagcttatga
    17161 agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc ctggatctac
    17221 gtttaaaatg attacagcag caatcggtct cgacaacggc actatcgatc caaatgaagt
    17281 gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt atcaagcaac
    17341 gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct attccgataa
    17401 tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta caggcttaga
    17461 taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag cacaaatttc
    17521 taatgaagat agctttaact cagatatctt gctagctgat actggatatg gacagggcga
    17581 acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca acaatggcac
    17641 acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga atgtcatcag
    17701 cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag atgtaaatgg
    17761 tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg gtacagcgga
    17821 aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg ctttcaatct
    17881 tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg atgattcagc
    17941 aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat aaaaaattgc
    18001 tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt caaataaacg
    18061 gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca taaacttttt
    18121 ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc acgataacta
    18181 aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt aaaatgatca
    18241 aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta tgttgagaaa
    18301 aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc gctacaagcg
    18361 tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact tctaatccta
    18421 cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca ttgtggtata
    18481 cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca ccaatgacag
    18541 agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact agcgatacga
    18601 tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa ggcgcaatcc
    18661 atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa acaagcggta
    18721 aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt agaactaaaa
    18781 cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct tttgtattca
    18841 ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc gcaagaataa
    18901 tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc aaccagccaa
    18961 aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg aaatattttt
    19021 tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagatc acaccaatct
    19081 ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct tggcctagca
    19141 aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc gagatatgtg
    19201 agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc attctcatca
    19261 ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa aaaataggaa
    19321 aatatactca caaaaaaacc aacaatccaa aagatttttt tggattgttg gtttttatac
    19381 ggtctatttt aataactaaa aatgattaac gtatagtcat ttttcttatt ctgatgaaat
    19441 tgataatctc ctggatgccc atgaaaatca aaataaagcc gaatatttgg aatagaagca
    19501 tcaaactttt gaatggattg aacaataaga cgaatccgcc aatcaacaac agaatactac
    19561 tataaacact ccaactttta gcttttgtat tcatgctgaa gaacaactgg aacaagctgt
    19621 tgatgataac agctaatcca agaagaatcg gcaggataga aacgattgca ggtgcaaaag
    19681 catagacgat cagagctaag atcaggaaaa aaagaccact gaacagaccg atactccaag
    19741 aacctgtttt ccttttaaaa cggtaagcct caaaaagatt caacaaccca agcaaaacaa
    19801 gataggcggt gatcagataa ccgataaaat gaaaaacagc agaagggttt agcacaatcg
    19861 caatcccaga aataatataa acagcagctc ttaataaggc gtatttttga atattttgtt
    19921 taatcgtctc aaacatataa gaaaacctcc tttgacttat tttaacgtga actagtcatt
    19981 ttaccaaatt aaatgttaca aaatgagcga aggagaagaa tatctctgga aaaatcaact
    20041 tctgaggatc ttttccattt ttttgccttt tgctagctca tcgatcattt tatctaaata
    20101 acgaatttct ttcatcaagt tatcctcgat gtcttctact cgtacaccac aaacaacacc
    20161 tgtaatcaag gaacgtgaag gattggggac aggggcttcc gcaaaaaatt gttcgaaagt
    20221 cacttgtttt tgtagttgat attctagttc tgtttgggaa taacccgtca gccaacaaat
    20281 gatttcatct acttcctctt ttatacgatc ttttctctcc gcttttgcta catacatagg
    20341 ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc gccataatca gtcacctttc
    20401 tttttttcaa attatttact acttatagta tataagaaaa agagaagaaa aagcgattat
    20461 tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc atcctttttt ccgctctctg
    20521 actttttaca atcttcatag ctcaagtgcg catcagataa tcgaatgctc ctaaagctgc
    20581 agtagctcct gaacccattg aaacaatgat ttgtttgtat ggactatctg tacaatcacc
    20641 agctgcgaaa acgccgggga tattcgttgc gccatttttc tcggtcacta tttcatttct
    20701 tggattcatt tcgatcgttc catctagcca atccgtatta gggagtaagc cgactagaat
    20761 gaatactcct gaaacagcta gtgtatgcat ctccatcgta cggcgatcga tataggagag
    20821 agattctacc tgattcgtac cgctgatttc tttcgtcgct gcatgtttga taacagtgac
    20881 attcgcaaga gagactagtt tatcctgtaa gatttgatct gcttttaatt ctggtaagaa
    20941 ttctagaaca tatacatgtt ttgctaaccc agccaaatcg attgctgctt caacaccaga
    21001 attaccacca ccgataacag caatctcttt tcctttaaat aaagggccgt cacagtgtgg
    21061 gcaataagca attcctttat ttttaaactc tttttcgcct ggtacattga tagaacgcca
    21121 gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt gatataccgt tagctaagcc
    21181 aatattaata aaatgttgct ctttatttaa ggaaacggcc tgttgattca tcataatgtc
    21241 gatagggtaa ctacgcacat gactttctac ttgctgcatc aattttggac cttctgtgta
    21301 agaggtacca atcatatttt caatccctag tgtttcaacg acttgtccgc cgaaagaatc
    21361 agcgacaagt cctgtacgga ttcctttacg tgctgcatag atagctgcac tactagctgc
    21421 tggtccaccg ccaatcacta atacatcata aggttctttt tcaaacaact cttcttcaat
    21481 caaagggcca gtgatttttt caacaatttg ttcgatcgtc atgcgtccgc tactaaattc
    21541 ttctccattt aaaaacacag ttggcactgc catgattttt ttatcatcga tttctttttg
    21601 gaacatccca ccttcaatca tcgtatgagt aatatttgga tttaatacag ctaatatatt
    21661 caatgcctgt acaacgtcgg ggcaattatg acaagtcaag ctaacataac tgtcaaaatg
    21721 caatgtttgt gttatttttt tgattttaag actcaaagat tcactgattt tcggtgcccg
    21781 tccactaatt tgtaacatag ccagtaaaaa agaggaaaat tcatgaccga gggggatacc
    21841 agcaaaaacg attccacttt ctttgccaac tggattgata gaaaaactag gtgtgcgcat
    21901 cgaatctctt ttttcccaag agattttagg agacatttct gctatttcct tcgtaaattc
    21961 agctagtttt tgactatttt cactattatc caaactaaca gaaaaaacaa taggagattc
    22021 aatcagtgct aaatattgtt cgagattttg tttagtttct ttgtctaaca tatccaccac
    22081 ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt tcgccacttt ctttccattt
    22141 tgccggacaa acttcacctg ggtgggtacg tacatattga gcagcgcgaa tcttatcaat
    22201 cagtgcacta gcgtcacgac cgattccatc tgcattgatt tccatagctt gaacgattcc
    22261 gtctggatca ataataaatg ttcctcgctg agctaatcct tgctcttcat ccaaaacatc
    22321 aaaaatacga gaaatctgat gagacggatc accgatcatc acgtattcga ttttaccgat
    22381 agcatctgat gtatcatgcc atgctttgtg agtaaagtgg gtatcggtag agcaagaaaa
    22441 gacttcaaca cctaaagatt tcaatgtact gtattgttct tgtaagtctt ctaattcagt
    22501 aggacaaaca aaagtaaaat cagctggata gaagcaaaga atactccagt tccccataag
    22561 atctttttca ctgacttcta aaaattctcc ttgatgataa gcatgtgcat taaattcttc
    22621 gatttttttc ccaatcaatg acatagtaaa acctccaata ataattattt atttataatt
    22681 attgtaattt agttggagtt gcaaagtcaa tggatatttt gttatttaat aaagattact
    22741 caaaaacgaa ggttaattat aaaaatagaa gaggtcgcaa gaaaatgcta ctgctggaaa
    22801 tactcgggat aacgttgagt aacagtttcc tgttctttca gcatcatgtg taagtgcaat
    22861 cccgtttgaa atcgagcttc ttctggtttt ttatcaacta aagccttgag taaatcataa
    22921 tgttgttcca tgatcgtttc ccatggaagc ccctgaatgg tcactcgtaa ccagcggaaa
    22981 cgttctaaat gggtattatg gtcatctaac cagttccaaa cttcttttct tcctgcaatt
    23041 tcgaaacaag cttcatgaaa taaattatct gcacggaaaa aagcgcgttc gtcatttatt
    23101 ttgactgctt tttcttgttc ttctaaaatc gtttctagca ctattcgtgt ttgtgtgtcc
    23161 aatttggaac aacattcaat catgatttgc tcttccaact gttctcggac aaagcgagca
    23221 ttttctgcag aaacaagatt aattttggaa acatacgtac cactttgagg tatcgtatac
    23281 acgagttctt gctgacgtag ttgaatcaat gcttcgcgaa tgggagtccg gccgatagca
    23341 agatgttcct ccagtgcctt ttcagatatt ttccttccag gttctaagtc agcatagata
    23401 atttgtttac gaatcgcttg ataagcttgt gattgcaagc tgcttttcat ctttattcct
    23461 ccaaaatcat ttctatgaac gtagtataac agaagaggtc tgaaaaacaa tctggtatgt
    23521 cacattaact agtaaatttg tataaatagt aaaattttgg ttgacatagc gatttttatt
    23581 gcataaaatt cacttgaata caaaaactaa tatgttagtt taggagggga aaaatgattg
    23641 atttaaacaa ctggaggaac tttatccctg aattatctca aaaagaagta gaaatacctc
    23701 attttgaccg tgcatcatta agagaaaatg gacaaaaaaa tcctgtctgg atccatttcg
    23761 gtggcggaaa tctttatcga ggatttcatg cagaaatcgc tcaacgttta gcggatctag
    23821 gcgaattgtc cactggtgtc attgtttgtg aaacattcga tgaagaaata attgaaaggg
    23881 cttaccaaga ttttgatgat ttgattcttg aagtggtcat gccaagcagt ggagagttcg
    23941 tcaaacgtat attagctgcg acagctgaca gtctgtattg tcatccagat tgttctgaac
    24001 actttgataa gatgattcga cactttgaaa atcctagttt acaattagtt acttttgcta
    24061 ttactgaaaa aggttatcga ttgaaagata caaataataa atatatagga ccgattgcag
    24121 tcgatataaa aaatggtcca aagaagccat cacatacaat gagtattctt gctgtgctac
    24181 tttataaacg ttttttatct ggagcttggc cgattgcact tgtctctaca gataattttt
    24241 cacaaaacgg tagtcaatta cgtacttctt tgctaacgat cgcaaaagaa tggcaaaagg
    24301 aaggttatgt acaaaataca tttatccaat acttatcgaa tgaagaaatt gttgcgtttc
    24361 catggacgat gattgatcgt atcaccccga atccttcctt gtcagttagc aaaatgttgg
    24421 caaaagaggg cttttcaaat atgtcgattt tccaaaccgc aaaaggaaat aatattgctc
    24481 catttgtcaa tacagaggac atccattatt taatcatgga agatcatttt cccaatggac
    24541 gtccgaaatt cgaaaaagct ggggtattct tgactgatcg tgcaacggta gataaagcag
    24601 atacgatgaa ggtgacgact tgccttaatc ctttgcatac gtctttagct attatgggtt
    24661 gtttactggg gtataggagc atagctgagg agatgaaaga tccagattta tcgctgctag
    24721 tttctgaaat cgggtacaat gaaggcctcc cagttgtgga gtctccaggt atcattgatc
    24781 caaagatctt tctggaagaa gtaattactg tgcgcttttc taatccaaat attcctaata
    24841 tgcctcagcg aatcgccacg gatacctctc aaaaggtggc gattcgttat ggtgagacga
    24901 ttaaaaaata tatggaaata gaagttaaag acccgcagac acttgtattc ataccattga
    24961 ctctcgctgc ttggcttcga taccttattg gcattgatga tcaaggaaaa ccattcatcc
    25021 cgagtccaga cccactattg acagaccttc agcataagct ttccgatatt caactgggta
    25081 cgcaagatac atcacatatt catgagaaaa tggctcctat tttgtcaaat aaagaaattt
    25141 ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat agaagaaatg ttcatcaaga
    25201 tggtagcagg accaaatgcc gtacgaaaaa cattacagga ttacttacaa gaacatggag
    25261 gaaatttatc atggaaatga cttttagatg gtacggatca caggaagaaa agattacctt
    25321 agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga tggatatacc
    25381 agtaggagag gtatggccta gggagaggat caaagaacta aaagaagaaa ttgagtctcg
    25441 tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta aaattggatt
    25501 accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact tggcagaata
    25561 tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga aatctgacct
    25621 tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg atattccaga
    25681 tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg gattgccggg
    25741 atgggaacct gagcgcttag ctcctatcaa atccttgctt gaagcctatg catcagtaga
    25801 cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc caacatgtga
    25861 agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga tttttggatt
    25921 agctcgaatc gtaaagaatc gagaagattt agattggttg tgtcgcatag tagacagccc
    25981 ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa atgatgtata
    26041 tgggatttta gcagaatttt gccaaagaga tcgaatccca tttgcacatg ttcgaaatat
    26101 taaattttta gctaatcgag atttctatga ggcaccacat aaatccgaat acggttcatt
    26161 agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacggat acatccgtcc
    26221 tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct tatatgatcg
    26281 agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag gaaacaaaag
    26341 taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg atgttcaaat
    26401 tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat cttatttggt
    26461 ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt cttttaattc
    26521 aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg tgatgattcc
    26581 ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc agtcgctgat
    26641 aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat ttgaaaaaat
    26701 ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat agttcacacc
    26761 aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt cttcaaccat
    26821 ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt gttctctgaa
    26881 atccatataa aattgttcaa tggcggtttg gacttctttc aatacgctat gttcttgata
    26941 aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc cattataaag
    27001 tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta taaacaaatc
    27061 aaatacagta caaagggact ccaccatttc tcttgaataa agaacatgag gaacgtttgc
    27121 tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt tcgtgataga
    27181 aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca gattaggtcg
    27241 caaaatatct acagagagat caataaaaaa gtcaatatat tcatactctg tgaattcttt
    27301 ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta atagaaaata
    27361 taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa aaaaagtatt
    27421 catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag tacttgaaaa
    27481 gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc cagaaaaaga
    27541 ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa atgcattaat
    27601 ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac tgaaactgat
    27661 aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga ctgattcgtt
    27721 gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt caaaattcaa
    27781 ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac gctcaaagtc
    27841 ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga aatgatctgc
    27901 taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa tatctaattt
    27961 tgtattgctt tttttcttca agataaacgt tttaatcgct tcttgagttt gcatgaaaga
    28021 ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt cataaattta
    28081 gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt gcatacaaaa
    28141 aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac atttgcagcc
    28201 ttaagcttat cgcttactat aaatcagaca gtccacacag aggagacaga aacagccaaa
    28261 tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga attttcatcc
    28321 ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa agcaaaaatt
    28381 gactattcgg gagacttagc acctatacta caatcagctg ttacatttga gaatgttgat
    28441 cccactttgt cgattggcta tgacgagaac actttgacta tccaacacaa gcaagcgata
    28501 tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt tactgtcaag
    28561 gtatcagatg gactaccgaa cgatcatcat ttaacgccgt atagtcagcg gcagacaatt
    28621 gagcaagatt cagccatcga ctcaggagga gatcttgttg aagaaccaac agataaacca
    28681 gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga acagaaaccg
    28741 gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga caataaatca
    28801 aaacctgaaa taacccctgg agaaaaacca gatgtaactc cagaaccaga tacggattct
    28861 ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa
    28921 aaaccagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga tacggacgca
    28981 agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa
    29041 gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct gaataaaaaa
    29101 tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc
    29161 tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag
    29221 ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat
    29281 ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa
    29341 aaagaaatat ccagaaaatg accccaaaat accagattga aatctgatat ttggggtcgt
    29401 tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt
    29461 tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa
    29521 tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa
    29581 tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat
    29641 ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag
    29701 ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa
    29761 tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg
    29821 tagtgagcaa cgtatttaaa agagcgacaa caaattgagc ttctaatacc aaaccaaatg
    29881 tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt
    29941 ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta
    30001 agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg
    30061 caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga
    30121 tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatta ataacggact
    30181 ggatcatgtc aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga
    30241 taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa
    30301 atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa
    30361 tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga
    30421 gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca
    30481 aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagca
    30541 aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca
    30601 tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca
    30661 gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg
    30721 gaataccttc tgcttgtttt tgcaacgcag caccttgagc agtcagcgta acatttacga
    30781 ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct aactttttta
    30841 gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag
    30901 tctttttctt ttcccataaa actaacatca caagatactg ggtataggta agattcagcg
    30961 gcttgaggag gggagtgtag tgtctaatga tttctttaga ggcggcatat aaaggaaaac
    31021 atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg
    31081 tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat
    31141 atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg
    31201 cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct
    31261 cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt
    31321 ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac
    31381 aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg
    31441 aatgtacgat cgggattatg cacttctcca gagcgtccgc ctgtgttgat cattttcgta
    31501 gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac
    31561 acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca
    31621 tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaatatcaga gacaaaaaat
    31681 aatctcatca taaacattac atatatttct caaaaagttg ttttctttct tcaaaaatta
    31741 cttcacgatg gacctgatga agcatccata aataaccaaa aggatctctg aacattgagt
    31801 tagtcacacc aaagtccatc atttctgtca tttcttgaat ctctacacag ccatttcgta
    31861 atgctttatt atgtgtgtcc tcgatatcag agacgatgat attgaaccag atcgtatttg
    31921 gatgttcagc agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc
    31981 tcgtaccata caaggtaaaa atgacttcat tattgcctct agggtaatct gttgcctcaa
    32041 tcagttgtat ttcatcaaag attgtttgat acagttctag tgctttcaaa ctatcattta
    32101 atacaaaatc gatttccgtt cctttatgca taaagatctc ctctcctttt cagtcgaaca
    32161 gttactagat tcttttatta taccagctaa agaaaacgca ttcaaaaaaa tagcggattt
    32221 acttaccaac tcattcgatt ggatgataag taaatccgcg tcccattact tctgctacgc
    32281 tgctgacaat gacaaaggca cttggatcga tttcttctat caatggtttc attcgggaaa
    32341 attcatgatc agtcacaacg atcaataaaa cctcttttga ctttcgatta tacccgcctt
    32401 ttgcctctag cagggtaagg ccacgtccaa tctcagcact taagcgaagg cggatgtctt
    32461 ccaaataatg ttcactcatg accataacag atttctttcg attgatacct gtttcgatat
    32521 aagtcatgac aattgaagtg attgcaatag ataaaatagc ataaagaaac tcttctattc
    32581 caaaaataaa cagattaaga gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag
    32641 tattcaagtg gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag
    32701 agttgttttt atataaaatc gcaactccta aagcaaaaat cgcacttcca aaaatgacag
    32761 acaacaaccg atctgaagta atcattgttt ctggaactat tgcaatgatt aaaggaaaaa
    32821 ccaagcttcc gaaaagtact ttaaaaaagg gttttttacc taaaaataga agagctagta
    32881 ccaacataac gatattcaac actaaaataa ctatagctcg gtcaaagcct aaagcagctt
    32941 ccagtaatat tcctagaccg ctgacacctc cagcagcaat atgatgaggc cctaaaaaca
    33001 tattgatagc gactcctaat atgaagaggg caaaaataat cttaatgtat gtccatataa
    33061 attgtctcgt ttctttcatt tattcacttc cttaattcat taataatact cttatcttat
    33121 tataacttac ttttcacctt aagttaactt taattaataa aaaaattagg ctagtctaaa
    33181 ttattatgtt tcattcttct agaatatact tctacgccaa aaactgttaa aaaactttta
    33241 ctttagaaaa ttggagaaat tgatcgctta agataaattc aggacactaa atagtacagg
    33301 aagtagacta aaacttttaa aagttttaag tctatctatt tggcaagttt aaaaaataaa
    33361 aattcggtat caaaaaactg ttaccgataa catttttgaa agcgttgtct tttgcttctc
    33421 tatcacttat agtgatgacg tcatattaaa acacaaggag gaaatcaatg gatacagctg
    33481 caatttatca tcgaccggaa agtgaatatg catttctata tacgaataca agatttcgta
    33541 ttcggcttcg tactaggaaa aatgatatca aaaaagtata tgtgctatgt ggagatccat
    33601 ataccataac aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta
    33661 ccaatgttca tgattattgg gaaattgaag tgacgagcga aacaagtcgc ttacagtatg
    33721 cgttccatgt tgttggggaa gataacacgg actgttttta cggtgatcaa ggaatttttc
    33781 cttatcgaga aaatgtgatt actgaaccaa atttttattt tcgaatacct tattttcatc
    33841 aaatcgatcg attcactatt ccagaatggg taaaagaaac agtatggtat caaatttttc
    33901 ccgaaagatt tgcaaacggg gacaaagcaa atgaccccga acacacttta ccttggggaa
    33961 gtaaagatcc agaccgagaa gactttttcg ggggagattt gcaaggtgtg atggatcatt
    34021 tggattactt gacggacctt ggcatcaatg gcatctattt ttgtcccata tttaaagcca
    34081 catccaatca taaatacgat acgattgatt attatgaggt ggatcccgct tttggcgata
    34141 aaaaactttt aaaaaacttg attgatgaag cacataaaag agggatacgt atcatgttcg
    34201 atgctgtatt caatcatatg ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg
    34261 gagaaaaatc gatctataaa gactggttcc acatccgttt tttcccagta gattcttatc
    34321 agatgactga cttaccagaa actgctgaaa atatcccata tgatactttt gcttttactc
    34381 cattcatgcc gaaactaaat acagcaaatc cagaagtgca aaaatatttg ctggatatcg
    34441 caacttattg gataaaagaa ttcgatattg atggatggcg tcttgatgtt gctaatgaag
    34501 tcgatcatca tttttggaaa aaattcagag aagcagttac agaaatcaaa ccagacatct
    34561 atatccttgg agagatttgg cattcctctc aagcttggct gcaaggggat gaattccatg
    34621 cagtgatgaa ttatgcattt acagattcga ttaaagatta ttttgcaaaa aagaaaatca
    34681 cagcttcaca aatggtcagt ggaatgaatc atcagcaaat gctttacaga gatcaggtga
    34741 acgaaggaac attcaactta ttagattctc atgatactgc caggatcttg accctttgcc
    34801 aaggtaacaa agaattgatg aagtctgtga tggcctttat gtttcttcaa aaagggtctc
    34861 cttgtatcta ttacgggaca gaaataggga tgactggtga agatgacccg gattgccgca
    34921 aatgtatgat atgggagaag gaggaacaag atctagagct attcggtttt ataaaagaac
    34981 ttgtttctct aaggaaacag ttgtctaaga ttatttctga aggttctact cagtggctaa
    35041 tagtaaatga tcgtgaagat aagctctatt tcacacggaa actagaggga cagatcatct
    35101 atgtttactt taatcagtca aaagaacctt gggtagtaga acaggaaaat gaggtgattc
    35161 ttagtcaaaa ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa tacggatttt
    35221 tgatcatgtg ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt
    35281 gtgcccatta taataagggt gtaacagcac gttgaataca aaggaggaaa tgaagatgaa
    35341 aaaaagaagt gtgaaacagt tgggtttagg gttaatctct ttaggaatct taggcggatt
    35401 agcggcttgt ggcaatggta actcttcagc agaaggggaa gccgatgaag gaaaaacgtt
    35461 aacagtttcc gttgattctg gatataaaga ttatataaac gaaataaaaa acactttcga
    35521 aaaagaaaac gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc agttggaatc
    35581 attagcgtta gatgggcctg ctggaaaggg acctgacgtt atgatggcag catatgaccg
    35641 gatcggtgcg ttaggacagc aaggacatct ggcggaagta aaactgggaa atgaagcagc
    35701 ttacgatgaa acagacaaag cccaagtgac ttatgatgga aaaatctacg gagaacctgc
    35761 agtaatcgag acccttgttt tgtactataa caaagattta gtcgatacag caccatcaac
    35821 gtttaaagat ttggagaatc tatccaaaga cagtcgtttt gcttttgagt cagaagcagg
    35881 aaaaaataca ggatttttag ctaaatggac tgacttttat tattcctatg gtttaattgc
    35941 aggttacggt ggctatgtat ttggtgatga tggcacaaat ccttcagata tcggtttgaa
    36001 taatgcaggt gctgtagaag gaatctctta tgcaacagat tggttcaaaa atgtttggcc
    36061 aaagggaatg caagacataa aaagtgcagg agacttcgcc agccagcaat tcatgtccaa
    36121 taaaactgca gcaatcattg acggtccatg gcaagcgcaa acatacaaag aaaacaatat
    36181 caattatggt gtagccaaaa ttcctactct aaataatgga cagccttatc aaccgtttgg
    36241 cggaggaaaa ggatgggtag tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg
    36301 gcttgattat gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg taaatgaaat
    36361 ccctgcaaac caacaagcta gggaaacagc aaaggctaaa aacgatgaat taacaacagc
    36421 tgtgattgaa caatatgaaa cagctcaagc gatgccaaac atcccagaaa tgggcgaagt
    36481 ttggacaggt gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga
    36541 atccgcagat gaagcagtaa aaacaatcag tgaagccata gaacaaaaat actgattaaa
    36601 atgataaccg tccattttta taaaaaatgg aacaggtgct tcagataaaa gtggcaagag
    36661 cgagatggaa aattatcttg ctcttgttgc cattatctta aaagcagttt gcggtttagc
    36721 aagtttagag ggggcattca gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa
    36781 atcacgcttt gttcacttat tccagggatg gggcagtttg taaatggcca agcttttaaa
    36841 ggtatcgttt ttttcgccat attcgtccta ttcattggtc agatgcttct gggcggattt
    36901 catgcattgg taggattggt tactttggga agtataccaa tggaagatca ttcattattt
    36961 attttgattg aaggcacttt gcaattcatc attacaatca ttttcttgat tttttacggt
    37021 ttgaatcttt tagacgctta tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat
    37081 cggacgatga aggaagtcat acataatata ggtgatgaag gttttcctta tctattgaca
    37141 cttcccgctt atcttctcat gatttttacg atcattttcc cagtcctagt tacattgttc
    37201 actgcattta ctaattatga cttcaatcat attccaccag ccagtttaat tgattgggta
    37261 ggggctaaaa acttttttag tatcttcttt ttaagctcat atcgtaatac tttttttgca
    37321 gtcttttcgt ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat cacccttggt
    37381 attctcacag cggttgttgc tcaccagaaa tttattaaat tcaaacgtat tttcggtatc
    37441 attttcttat taccatgggc ggtcccagct tttattacta tcatgagttt ttcgaatata
    37501 ttcaacgata gcgtaggtgc catcaatact caagtgattc ctttcataaa ccagcttcct
    37561 tttgtagata tcagtgcaat ctcttggaaa acggacccga actggacgaa agtagcaatt
    37621 atcttagtgc agggctggtt aggatttccc tatatctatg tgatggttac aggaatctta
    37681 caatcgattt cagaagactt atatgaagca gcaaaaatcg acggagcaaa tgcatggcag
    37741 cgcttttcaa acatcacgct tcctgctgtc ttcctagtgg cagcgccaac atttattaca
    37801 cagtatacgg ggaatttcaa taatttctcc atgatctatc tgtttaacaa tggcggtccc
    37861 ggaagcgtag gcggaggagc tggttctact gatattctta tttcctggat ctataaactt
    37921 acgactggaa cttctccgca gtattctatg gcttcagcaa tcacgttgat tatctccgtc
    37981 atcgttatct gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa tatgggggat
    38041 taacaatgaa aaaatttcat tcgagtgctc gattccaaaa acgactttct cactttttta
    38101 cttacttttt tcttttaata ctgtcagtca ttatcatcta tccgcttttg atcacagcaa
    38161 gtactgcctt caagtcagga aatattgcag cttttagttt agatctttca agtgactgga
    38221 ctttaggaaa ctttactcgg ctattcagtg aaacacttta cgggacttgg tataaaaata
    38281 cattgatcat ttctgtccta acaatgatcg tacaagtgac tgtagtaact ttggcgggct
    38341 atacttatag tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc tttttagtga
    38401 ttcagatggt accgactatg gcagcattga ctgcctttta tgtcatggct ttcttattag
    38461 aagcgctcga taagtactgg ttcttgacaa tgatctatat aggcggtgga attccaatga
    38521 atacttggct gatgaaagga tactttgaca ctgtaccaat agatttagat gaatctgcta
    38581 aacttgatgg ggcagggcat ttcaggattt ttttccagat tgtattaccg cttgtcaaac
    38641 cgatgctcgc cgttcaagca ttatgggctt ttatgggacc ttttggggat ttcatgttag
    38701 ctaagttcct gcttcgaaca cctgaaaatc agactgttgc tgtcggctta caaacattta
    38761 tttctgatgt gacaaaccaa agagtctctc tttttgctgc tggggcaatt ttgattgcac
    38821 taccgatttg tctattattc ttctttttac aaaagaactt tgttaccggc ctgacatctg
    38881 gcggaacaaa aggataggag cagagtatca tgcaaacatt tccgttaaac tatttttccg
    38941 cattacgtac gccaactcaa gtgtttgcgg gacgcaaact attatcatgg cctaaatttt
    39001 tcttgatatt tgtattttta gtttccttga tggtcatgcc tgtgactctt ttttatgcca
    39061 atcagatcca agcgattcca ttagaacaat ttctatccgt tcattcgctg atcgatgaac
    39121 aaggaaccca aaaattttct gaacttgaat tatctgaaac tgggttacaa gcatcgcaac
    39181 aaacgatagc tgtgacccct gaaattttgg taggtgtgtc tttatctgag aaacaacaat
    39241 ccgatcatgg aacttttatt gattttgaga aggaacaatg ggtgatccaa caaaaggata
    39301 aatctggtat acgccgctat acgatgaatt atagcccatc tttccaacca gattctgtac
    39361 gtacgcccga agatttccaa cgatttttag aaagagaatt ttatgccagt aatcgtccaa
    39421 caatcatttt atcttatagt ttaagtcttg gtcttgtcct atttgtcatg acgagtctca
    39481 ttttattcgg ggcttccttt ttcttgtgga tgacaagaaa aagccaactt tcatccattc
    39541 atacattcaa agagagtgca aatttgatgc tgaatgttat gggaataggt tccatggcag
    39601 cagcagttgt agggtttatc cactttgact ttatcttgat gctaagtgtc caaacaacga
    39661 taactgtctt gctgctttta tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg
    39721 aatgacaagt ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag
    39781 tttttgaaaa atttctgaaa aaattaaact tcttagttga ttcttttctt ttttagtatt
    39841 aacgtaatag ggtagttttt ttaaggagga aaatcatgtt agagaaaact ttatacaatc
    39901 aattattttc acgttctttc tcgctccctg tcgaagtaaa ttattgggac ggtacaacga
    39961 aacaatacgg agatacggac actcctcccc aaatcaaaat caatatccat gaagaaatcc
    40021 caatgaaaga gattacaagt aacgcatctt tggcactagg agaagcctat atggatcatc
    40081 gaatcgagat cgagggaagt atccaagcat tgatcaacga tgcttatcag caagcaaaca
    40141 gctttttacg aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac
    40201 aaaaaaacaa agaagatatc catgcgcatt atgacttagg taacgacttt tataaacttt
    40261 ggctagatcc aactttgact tattcttgtg cttatttcaa tacacctgaa gatacgttgg
    40321 aacaagcaca aatcaataaa gtccatcata tcttagacaa actctttatc aaagaaggcg
    40381 aaacgttgct tgatatcggt tgtggctggg gaacattgat gttcactgct accaaagagt
    40441 ataatgtcaa ggcaactggg atcacactaa gtgaagaaca atacgatttg attaccaaaa
    40501 gaataaaaga agaacattta gaagataaat gccgtgtatt attaatggat taccgcgaac
    40561 taaaaggcga aacgtttgat catatcacaa gtgtaggtat gttcgaacat gtaggttctg
    40621 aaaatcttga aggttatttc aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt
    40681 tgatccatgg aatcagccga caacaaggcg gtgcgacaaa tgcttggatc aataaatata
    40741 ttttccctgg cggttatgtc cctggtgtta ctgaattagt tgataatatt acaaaaaacg
    40801 atttgcaact gattgatttg gaaagcttac gtcgtgacta ccaattgaca ttagaacatt
    40861 ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt gattccttct
    40921 atcgaatgtg ggatctatat ttacaagctt gtgcagcttc atttgaatca agtaatatcg
    40981 atgtgattca atatttgctt gttcaacctg gaaacaatga catcccaatg catcgtgcat
    41041 aattgaggca aaagtactgt ttaaagacta tttaaaaaga agctaagttt tatgatgctc
    41101 cagaagtgaa cttttttgtc taacttctag gattgtctct aaagcttagt tt
//
LOCUS       DAFELO010000018        39146 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFELO010000018
VERSION     DAFELO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.018"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..1350
                     /locus_tag="LP452_001049"
     CDS             <1..1350
                     /locus_tag="LP452_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2013792.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="LP452_001050"
     CDS             1484..2827
                     /locus_tag="LP452_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2013793.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="LP452_001051"
     CDS             2939..4390
                     /locus_tag="LP452_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL2013794.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="LP452_001052"
     CDS             4739..5371
                     /locus_tag="LP452_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL2013795.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="LP452_001053"
     CDS             5959..7398
                     /locus_tag="LP452_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2013796.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="LP452_001054"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="LP452_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="LP452_001055"
     CDS             8160..8375
                     /locus_tag="LP452_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013797.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="LP452_001056"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="LP452_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="LP452_001057"
     CDS             complement(9035..9946)
                     /locus_tag="LP452_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2013798.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="LP452_001058"
     CDS             complement(9965..10969)
                     /locus_tag="LP452_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2013799.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="LP452_001059"
     CDS             complement(10966..13089)
                     /locus_tag="LP452_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2013800.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..15541)
                     /locus_tag="LP452_001060"
     CDS             complement(13094..15541)
                     /locus_tag="LP452_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2013801.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15528..15917)
                     /locus_tag="LP452_001061"
     CDS             complement(15528..15917)
                     /locus_tag="LP452_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2013802.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15977..16480)
                     /locus_tag="LP452_001062"
     CDS             complement(15977..16480)
                     /locus_tag="LP452_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2013803.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16493..16717)
                     /locus_tag="LP452_001063"
     CDS             complement(16493..16717)
                     /locus_tag="LP452_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013804.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16821..18731)
                     /gene="ltrA"
                     /locus_tag="LP452_001064"
     CDS             complement(16821..18731)
                     /gene="ltrA"
                     /locus_tag="LP452_001064"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2013805.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19477..19614)
                     /locus_tag="LP452_001065"
     CDS             complement(19477..19614)
                     /locus_tag="LP452_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL2013806.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19611..20795)
                     /locus_tag="LP452_001066"
     CDS             complement(19611..20795)
                     /locus_tag="LP452_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2013807.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(21054..21344)
                     /locus_tag="LP452_001067"
     CDS             complement(21054..21344)
                     /locus_tag="LP452_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013808.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(21334..22476)
                     /gene="dcm"
                     /locus_tag="LP452_001068"
                     /pseudo
     CDS             complement(21334..22476)
                     /gene="dcm"
                     /locus_tag="LP452_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22552..22716)
                     /locus_tag="LP452_001069"
     CDS             complement(22552..22716)
                     /locus_tag="LP452_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013809.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22709..23383)
                     /locus_tag="LP452_001070"
     CDS             complement(22709..23383)
                     /locus_tag="LP452_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2013810.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23461..24801)
                     /locus_tag="LP452_001071"
     CDS             complement(23461..24801)
                     /locus_tag="LP452_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2013811.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24884..25219)
                     /locus_tag="LP452_001072"
     CDS             complement(24884..25219)
                     /locus_tag="LP452_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL2013812.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(25229..25603)
                     /locus_tag="LP452_001073"
     CDS             complement(25229..25603)
                     /locus_tag="LP452_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2013813.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25616..25930)
                     /locus_tag="LP452_001074"
     CDS             complement(25616..25930)
                     /locus_tag="LP452_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2013814.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(26043..29270)
                     /gene="fss3"
                     /locus_tag="LP452_001075"
     CDS             complement(26043..29270)
                     /gene="fss3"
                     /locus_tag="LP452_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL2013815.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(29684..30850)
                     /locus_tag="LP452_001076"
     CDS             complement(29684..30850)
                     /locus_tag="LP452_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2013816.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            30875..31201
                     /locus_tag="LP452_001077"
     CDS             30875..31201
                     /locus_tag="LP452_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013817.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDAGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            31354..32406
                     /locus_tag="LP452_001078"
     CDS             31354..32406
                     /locus_tag="LP452_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2013818.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            32482..32700
                     /locus_tag="LP452_001079"
     CDS             32482..32700
                     /locus_tag="LP452_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013819.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            32687..35260
                     /locus_tag="LP452_001080"
     CDS             32687..35260
                     /locus_tag="LP452_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013820.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            35251..35448
                     /locus_tag="LP452_001081"
     CDS             35251..35448
                     /locus_tag="LP452_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013821.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            35452..36063
                     /gene="mobC"
                     /locus_tag="LP452_001082"
     CDS             35452..36063
                     /gene="mobC"
                     /locus_tag="LP452_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL2013822.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            36048..37439
                     /locus_tag="LP452_001083"
     CDS             36048..37439
                     /locus_tag="LP452_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL2013823.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            37655..37969
                     /locus_tag="LP452_001084"
     CDS             37655..37969
                     /locus_tag="LP452_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013824.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            37962..38456
                     /locus_tag="LP452_001085"
     CDS             37962..38456
                     /locus_tag="LP452_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013825.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            38524..38838
                     /locus_tag="LP452_001086"
     CDS             38524..38838
                     /locus_tag="LP452_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013826.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            38937..>39146
                     /locus_tag="LP452_001087"
     CDS             38937..>39146
                     /locus_tag="LP452_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2013827.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT        12473 a         7257 c         6980 g        12436 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    14041 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    14101 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    14161 taactgaacc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    14221 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    14281 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    14341 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    14401 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    14461 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    14521 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    14581 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    14641 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    14701 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    14761 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    14821 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    14881 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    14941 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    15001 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    15061 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    15121 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    15181 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    15241 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    15301 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    15361 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    15421 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    15481 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    15541 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    15601 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    15661 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    15721 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    15781 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    15841 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    15901 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    15961 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    16021 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    16081 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    16141 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    16201 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    16261 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    16321 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca attcgttctt
    16381 tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca ccttcgttat
    16441 atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc ccttacgcac
    16501 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    16561 atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac tcccgcttaa
    16621 atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattgg gcattggtta
    16681 aaaaccattg atatatggat aggtaagttc ctgatattat aaatatctca aacttttccc
    16741 cccattcaca cggagcgtgc gactttcacc gcactccgcg ttccatcgtg tttatattga
    16801 aaagtattta tgtagtagac ctatatcaag tttaattctt gattgccagc taatactcta
    16861 tatttattta gtcttatgag ctggacttta tttagcttca atagagctat atatttttgt
    16921 attgtttctt ctgttttagc gtgtattaga cggtgtaccg cctcttttac caaaataaga
    16981 tttttataat cgtcatttcc accagctcca gttggtacaa tatgatgaca atgtatttca
    17041 ttagcttgtt gaaattcttc cagagttata gcacattttc catgttgagc agaaaatagc
    17101 gaaattcgat tgtctgcgta ttctattgtg cgattttcca aagataattt cattaactgg
    17161 tacatcagta atttatcaac acttagattg gtgtggataa attttcttcc ctctagagtg
    17221 tagcaattcg ctaaatacac ctttgccatt ggttttctgt gttggatata tccaacaggg
    17281 aaaataggtt cgccctcgcc agcaacatac cgaagcattt tagatttacc atatcttgtt
    17341 gcctcctttg tcgtaagttg tctgcctgtt tttctcaatc tgcccctttt gtttgtacct
    17401 aatctgttag tgaagatagc cattacagct ctattcagtt tcatacaatc cagatttaca
    17461 ttcgttgcaa tctgataata attttgcata cctaatacca ttgaattata aagtcttgtt
    17521 tcttctaatt ccgttttccc ttttgaggga gaagcaattt tctttgcctg tgataccagc
    17581 ttgtcttttt ggtttttgaa ttgtttatca cttatatgtg atttaacaac taacttcttt
    17641 cttctcggta tcattttaat cttgaaacca agaaagtcgg agtatctttt tctaacattt
    17701 acaattcttg ttttttcttg tgatacttcc aattttaatc gttgttcaat ccattgagtg
    17761 attgctattt tggctttttc agcactttcc ttgtagcggc agaaaactct aaaatcatct
    17821 gcataacgaa ccatatacat ttcctttaac tttgttttct tcatttcttt gtaagcgttg
    17881 cttttacatg ggctaccgct tttattgata tgaaccacat agttttttgt aacaggattt
    17941 tcctgccatt ggctttctac ccaatggtct aactcattaa gaacaatgtt tgctagtagt
    18001 ggggatatta ttcccccttg cggagttcct ttctctggat atgttattgt accgttagtc
    18061 attttgattg gagcttttaa tatccttttt aaaatgaata tcaagtgctt gtcccgtatt
    18121 cccatagccc atatctgttt tatcagcttg gaatggttca cgttgtcaaa gaaaccctta
    18181 atatcaaatt caatcacata atgtagctgg ctgatttgta ggcggttata ggttgctttt
    18241 attgcatttt caactgacct attaggtcta aaaccatagc tgttttcact aaatttagct
    18301 tcgcagattg gttctaatac ttgttttata cattgttgta taagcctgtc ccacatacaa
    18361 ggaataccta gaggtctggt tttaccattt ggttttggta tttcttttcg cctcactggc
    18421 ttaggtcgat acccatgtgg actgcctgca acaatgtatc ttactttttt gacaactgtt
    18481 tctgctggca atttgccaat atctttaatt attgtaccgt ctgttcctgc tgttttactg
    18541 cctgcatttg ctttgatatt tcgatatgcc agtaatatat tttctctcga taggataata
    18601 tccataagat gagtgaaaat atctccattc ttacttttgg catataaatc atcaaaggtg
    18661 ttttgcattt cgtaatattc agcgtgtctt aaatcttcta cacacaatgt ttcattttta
    18721 tttttaggca taaggcttca ctcctttact tgaaattgag tgagcttttt tatcttactc
    18781 gtaatcctta ttgaaacaac ataatacttt tctaatatat acacgacttg agggcattcc
    18841 tccattctca ttacaagaat ttcttaggtt cgccctctgc ttttcagcat tagttcaact
    18901 gcttattgtt cttcgtcagt ttattctctt gatgagaacc caataccttt cctcgttccg
    18961 ataatcttat ctatacatat atatccttag gtcactagct ataagcctgc tggcttgata
    19021 ccgcctgtaa cgttatatgg catttcataa cctatattct tactttgcca caccagctac
    19081 gatttctcgt acctttgcat ttctacaaag ttcaaattta gacccgtaca ttcgctgttt
    19141 cgtcagttcc ttatagaaca ttctaaccat agatatttta tagacccccg acctgttgga
    19201 tacgctaccc acctctggat agttttcgat agaagatata tgttcatcaa ctattacggt
    19261 atctttcagc cgtctttacc gagcttcaaa cattctgagt ttcctcatac aatgcttgtc
    19321 ggaggctcag ggcaggcgtt tcagagcgtt acttcatcat tccacctgtc agattatcag
    19381 ttctcctaat aaccaaactt tttcgttctt ttattagtgc cttagctttt cactaaggaa
    19441 cgagtcgcac ataaattttg tccgaaattc atcttgtcac tttcctttct tttccattgc
    19501 tcgatccgct gctgcacttg cattcatcaa gcacatcaca gtaacaccaa gcatactgcc
    19561 accaaatacc aaaaaaatat cttttacaat tcccatgacg attcctcttt tcatataatc
    19621 aaattttcaa ttgccatact ttgttgctcc aaaatctttt tatgtcggtc tgataagcgt
    19681 gcattctgta ccatatcctg gatataggtt gtccgattca atcgatccaa ctccttagcc
    19741 attttccacg taggggcgac ttgtcggcct agccaattca aggttcgttc aatggtatac
    19801 ggttttggct cggaagttaa ttgaatctct ttccgattcc gtccaataaa acgcccccat
    19861 tgttcattca agggccagtt ggttcgcctt ttaccttcta ctttatccgc aaatcgcaag
    19921 taacgattga taatggaaaa cgtggtgctc tcaatgctac gaccttttaa caaatcttga
    19981 atcgcatgaa aagcacggtc atttttcagt cgaatctcaa aacgattttt cgtctcagtt
    20041 tggtctaatg gaatccctaa cttgatatat tgttcatagt ctttttcata caaacagaaa
    20101 taaacctcac tcttgagact gccgatatat aaggtattgc ccatgccgtc cttttcatca
    20161 gcctttaaaa gttccccgga acgataactt ttaaaggtac gaaacaagga gatacattct
    20221 tccttctgac attttttcgt tagctcaggt atatccaata gccccactcg atcattgatt
    20281 gccaagtcca agcgtttcat cacgccaccg gcctttagac aatcctcgaa aaagtcgtac
    20341 cagctacgct tttgagcgag taaaaaagtt tcaaattgtc ggcaacctcg cccctttaat
    20401 tctaaaagca cacctttatc ttcttcatga gacaacatca ccacaatatc acccatgaca
    20461 tactgttctt gataggagta aaaggcataa tcttcatgga gcatatactc aaatttcaaa
    20521 tggagaattt cttgaataat ttttcgtgca tccgttgtgg gaaagcgaat ccttacatag
    20581 tcaaacagta acgtcaaagg caaatcggga ttaaagcttt ccagttggtc aaaaattttc
    20641 ctcatggttt ttatagatgc ggttacgcca ccattttcta tctgatttaa gtacggccga
    20701 ctaagtccac aagccgtagc caatttattt tgtgaaacgc cgtattcgtt gcgtcggtct
    20761 tttaatagcc gataatctaa attccgttgt gccaagttct cacctccaaa ttcaaggtgt
    20821 taactttttc aaatgagtta acacctatac aaattactta ctatctttga tacaacaagc
    20881 attcttaatg gttgcttact tattttttgt gtttttgtac ccccctgtta gatacggggg
    20941 tgagacatca tggtcggcta acgccgtcca tgaacaggca cagcaccacg gaggatttct
    21001 gcttcgcgaa tcctccgcgg tcagcgccta acagtcagtt gtgttggttt aggttactca
    21061 tctgtaatac catttacttc caactctgct ttcatttcta acaagtattg acgaatatct
    21121 ggttcttttg cttggtccgc aatccgctca aattctgcaa ttacttgatt tatttcaatt
    21181 cgagcatccg tctcctcatt cattaaatat ggatcgccag cgactttgac accattcttg
    21241 cgaatatcaa attttaaccg tcgcaattgt tcttctaatt ctgggtctga ctgaatcttt
    21301 ccaatcaccc aataaatttc tccaatcaac tcatcataaa ccatgtacat ccaactcttt
    21361 taatcttctg gcaatatcaa aaatgaccgg aactgttaca gagtttccgg cttgcttata
    21421 caactgacta tctgaattga cttgagaatc tcgatcaaaa gcccaatcgg gaaagccctg
    21481 aagtcgccaa cactctctag gtgttagctt ccgaatttga tactgatccg tgacgatcgc
    21541 ttgttggtct cctgttagta aggtatgaac actctgtttt cccagacgac ctcggcgact
    21601 tgtggaatta ggaaaagcca gattcacact gtcttctggc ttcacctctt gatattctgt
    21661 ttcatttttt cgaatcatga tttttgtacc accttgtttc gttgacaagg tgggggcggt
    21721 tccagtaact tcataaactc gattaccttg ttcaaacttt cccggtaata gtcctgctac
    21781 agagattcca tcccttgctg gcagtgcgat tttgattcct tcatcacctg ttaaggttgg
    21841 cgctacgcca tctgaaccat agacttgtcc acccattcct cgattggaag gattgatatt
    21901 taccaaattt ctgatgtttt gatcagatgt tgaagacggt ccaatgatag gaaatacttg
    21961 ttcggtacat gcgtctcgag aatgtccgat aaggaatatc cgctcccggc tttggggaat
    22021 gtaggccttt gagttaagca cctaccactc cacgtcatac cccaatccat ccagcgttcg
    22081 gaggatcgtc tcgaacgtag cccctccttc gtgattgagc aatcctcgga cgttctcaag
    22141 gaataaaagc ttaggtcgga gaagagtggt gaaccgagcg atttcaaaga ataaagtacc
    22201 tcgagtatcg gaaaatcttt gccgctttcc cgcaattgaa aaagcttggc acggaaatcc
    22261 gccgcaaagg atgtccactg gtccgaggga ttgaataaac tcatctgata cacttgtgat
    22321 gtcatgcatt tccacctcct tacttgtatc atgaattgct ttataactac gtctagcaaa
    22381 tttatcaatt tcacaaaaac cgatacaatg atggctcgcc tgttccatac ctaaacgaaa
    22441 gccaccaata ccggcgaata aatctaaaaa tctcatacat tacctcattc attgcaaaga
    22501 aaaaagccaa gaacattttg ctcttggctc atacattatt aatttgtcct actatttttt
    22561 atttaatcca actaatttcc gttgtttttc attggcctga tcggctacat atttaatcac
    22621 cgttttgaac tcttttgcag tagcattttc cgataaatcc gcaatgctct tattgtattt
    22681 ctttacaatt ttcgttactt gttctttctc agtcatgtaa ttgaacctcc aaatctttcc
    22741 tactataccc tagtggtaaa aataattcta gattatccgt agcataatga acttcggaaa
    22801 tattattcgt ataatctttt attatgatat gaagcaatac atctggatgt ttactcttta
    22861 cttttgcaat gttctctctt gatttgaaaa aagcgttaat aaatgccttt ttcggcacat
    22921 gacgtccctc tgcaatttca cgttcttgtg taaatttcca agcgatttta gggtcttgat
    22981 aaacatagaa aatcgtcact cgaaaatgtt ttttcaacgc ccgcgagata tttttttcag
    23041 cacttaatat tgcaaaagtt gcatccagaa taaaagaata gccatgatct aagacatatc
    23101 taaatgcctg ctctaccaac caagaagatg ctttttgaaa gagactagag ttttttccat
    23161 tataatcagg aaactgggtg cgaaagtaat ccgcatcaat aatcacatgg ttatcataat
    23221 tctcagctaa tccaatggca acctctgatt ttccagcccc aggactccct gccataaaaa
    23281 ctgcgtctcg tccaacttcg ggggatttcc ctttgataat ataagaaata aacttatcct
    23341 tatttgcttt tgcatactct aaataagaat cattactttc catattaatt atcccctttc
    23401 ttcctactat ataaaagtat tatacgttac ttgaagtata aagtcagtat tgaagatcac
    23461 tcaatcattc atcttttcta atttactaat tgaatctaag aaatcatacc cttttggtac
    23521 aagtggcgta taaaactcac taatcacact gccacctgta tccacataac ctcgaccttt
    23581 gatgtgcttc ataaagaagt ttttttcgac ctccccaaac atcatggaat atccgagttc
    23641 actcatacga cccaaagcca cgcgaaagtt gaattgatcg cggattccgt ctcctaaata
    23701 tttcgcatcc ggcctttggc aagccaaaat cagaaagaaa ccagattgac gacccaacat
    23761 cacgatttgt ttgagcttat tcaaaatcac cgcactttct ttcgtcgtta acatctccat
    23821 ataagccacg tattcatcaa agattaaaaa gttgggtgga agtccaagat aggcataatt
    23881 ctctcctgtt ttgtagttag acatttcttt cattgccttg ctacgagcca tcatacgttc
    23941 atagaaatct tccacacaag cagaaatttc ttccttttgt gaataaacat gaggcatcac
    24001 cgtacctaaa tcagctaaat ctgcattttt gggatcgagg ataaacaatt ctgcatctga
    24061 cttcaatagt gcttcgataa tggtgaggag gaaataggtc ttccctccac ccgtgccacc
    24121 agcgattaac atatggggta aagaatcata agcccatact tgatttttca tcaatcgcag
    24181 agtcccattt tctgccacta cttcgtctat cccaattcga ttggcgatca tatcatacag
    24241 taaagtatac tccacatagg agtctttgag ttccttttct actaactcac aatacagtcc
    24301 gctttctaac ttcttttcca attttaaaag ctgatcttgg tatttcccaa gtgaaatttg
    24361 aacgcgaata gacagtaatc catctttcat tcgataatag attttgggga agtaactgat
    24421 agtctctctg gttcggctac tattcaaatc tttgaaaaag ccttcacttt gagtttgttt
    24481 cacttcatac caatggttct ctagaatcat ccgagctagc ttttgtcgat gccacaagct
    24541 tcgccaatga tcaagaaaaa agtgaatata aagcaatatc atagctcctg aaaaagcgag
    24601 acttccaagc agacatacta aatcttttat agaccaaacg atttcaatag tagaaagtga
    24661 tagcttagaa atatcaaatt gtctaaactc ttgaaaataa aagacgctca tccaaccgat
    24721 gaacagagcg agtagccagc caagaacaaa gtgataaacc aagtgctgat ctcctgcgcg
    24781 tatgcgatgt cctttgtaca ttttgatggt ttccacctcc aatcgaaaaa gcccaatagg
    24841 atttcattag gcaatttcaa aaagttatgc tagtcattta ctgctacgag gaggggagtt
    24901 gtttcgtttc aaccaattga tttaaaaagc cttcaatagc atccggcatc tccacaccaa
    24961 tttcttccaa aatggattct gcctgccgac ataagttttc atttacttcg acaactataa
    25021 agaaagactc ttcttctcct aaccagtcta gtacatcaga gtcttcttca ctgtcgctat
    25081 tttctccaag taaaccttga tgtataatca gaaacatttt ttcatcagag gttaatgctt
    25141 taacgagttg ttcctcagtc agctcgcctt ttccatattg atcgagacaa tctaaaatat
    25201 gttcaacatt aatttccata atcttcacct atttctttct ttcattttgg ttcgtaggat
    25261 ttccttgatt cggctgccgc ttcaagacta aatcatccgc cttaatgaac caattaacgg
    25321 tagttccttc tcgaaaaaca taattggcaa cggtatccac gattggattg accaactcta
    25381 cctctgcatc ataagcaaac tcgcgtaagg gtacattagc tgggatacta acttgaatca
    25441 ttcgcccttg tgcagaggat tttaaattat aggttcgttc ttttacctca ttaccaattg
    25501 ttccatcctc attttgcttc cgtacttctc ggcgtaatgc ggaaaaacgc aacttcccaa
    25561 atgttaaacc tgaatctacg acaatacctt ctgttaatct catcttcttt tcctcctatg
    25621 ctttcactaa atcatcaaca tacaacacgt aatcagtaaa cccacgattc tcaatctcat
    25681 agcctttggc aaccaaatgg ggattcacgg cttttagcgg ttggtcttga tcaaaatcct
    25741 tttcaccggc ttccgcagct actaccacct caatatcatc ggcccgttgc ttactggaat
    25801 acaaatgata actgcgacca atcaccgtag tattccgtcc atatccttct gttataattt
    25861 ctccttcacc ggcataactt aactttccaa aggtttcagc catatctggc acgacaaatt
    25921 tcaattccat cttcttttcc tcttttcatt gttagttttg gatacaaaaa tagcgcgact
    25981 acttttttag gaggcggtcg cgcacgttgg ttcttaggat ttacttgtat catctcatca
    26041 ccttagttcc ggcgattcca gaaataatag cccgccgtcg caaaaatcaa agtaaaacca
    26101 atgaacagta aaacattgga atttttctcc ccggtttgag ggaaggtctt cactgtggga
    26161 ctagattctt gagagccact tggaacagtt ggtgtttccg gttgtttcgg cgtacttagt
    26221 atggttggaa cttcttttgg tgttaaggtt tgagattttt ctttcaaatc ttcattgtgt
    26281 tttccattga tattcccatc tttatcgtaa ttgatttccg caaaagtaaa cttggttcct
    26341 tctggatatt ttccggtatc tacttttttc gcaagcacag ttttggtgaa ttctttatca
    26401 tttacttcat aatcaatctt gccagatttc caaatttctt tgtttgtacc atccggtaat
    26461 aaagcataca gaattgtttc gaaagcttct tttgagccat ccagtacatc atgagtaatc
    26521 gacacatcat caaacatatc caccacatca ccatgagtaa aggtctgaga accgttttct
    26581 aggtgggcct ttgtttggat agaaacatgg tgttccactg tacaactgac agtttgggct
    26641 tggttattca agctaacatc cttcgctact ggcttatcgc ctgcttcaaa agcttcctta
    26701 ttttcataca catagttgaa taagacaata gttttattta aaacttgttc ggcggttaat
    26761 ttatgctgaa ttttccaagt tcctgttttc tctttgttcg ccaacgttgc gcttgtttct
    26821 gcaatcacca ctggtttggc tttttcatct ttttcttggg cagcttttgt cgcatccaca
    26881 tcaatggctt gcgctaccac ataccagtct tctttgattt catgtagatt gtaactcaat
    26941 ttatcaacta gctcggtaga atccagagaa gttaattctt tgtttccgtc tttccaagtc
    27001 gcaagagaag ttaaactatc ttcttcctca ggcaaatcat agagcattaa acggttcaaa
    27061 ctaacagaaa atgctttgtt cgtcagcttc tcgtaaggaa cggtcaccgt tttaattggt
    27121 tgtttttggt cttgttcggt aatggtaaag acatactcac tcttcacaaa gtcttctttg
    27181 ttttccttaa aagtagaacg gatttctaat ggcgtaatct tttgaaaacc ttctggggct
    27241 tctacttctt caagtaaata atccccataa ggaagatttt caaacttgcc ataaccatca
    27301 aaccctaatt gctcgttata agctgtggtc gcttcatctt cggcacctgt gatttcattg
    27361 gtcccttcca atggcgacac tttaaaggtc agatcattga agccagtttc ggtagtgccc
    27421 gcagctgatc cagcaaattt aaagaaatca aagccaaagc gaatcacttg ttcttttgcc
    27481 gtaacatttc gggtaatcac ggcatttttt tcgctatcat ccaccttttt gatggataca
    27541 ggatactttg tttcatcaag gctatatcct tcgggcgctt ttgtttcttg ccaataatac
    27601 tcgttgatag ctaaatgttt aacggcaact tggttctttt cgtctaaagt caaagtgacc
    27661 gtttcatcag aacctttcgt tcccttcgct aactctggtt taaaagcctc actccattta
    27721 acagcttttc catccttagc agtaaacaag gtgtattcag caccctcaaa gactgcctta
    27781 ccttgtgctt tatccccagt atctttgtct tctttcgtca aagtggtttc gccagtgact
    27841 tcttgatttt gcccttttac gttactggta acaagggcta cggtttgatt ggcatacttc
    27901 aattcgactt ttacaggttt gaacgtattc acgaacccat gactagcttt agtttcggtc
    27961 acataatagg ttcccaattc caacgcatta gcaatctctt ttggtgtttc cgcatgaccg
    28021 ttttcatccg tggtcatttc ttgaacgatt tcaccggttg gactgtcttt gcgaatggca
    28081 aacgtattac cagctaagga ataattgtca ttccaaagat cactccctgt ttcaactccg
    28141 cttttatcca gaatgatttg ccctttttcc cgtgcgtttt ttgaagtcac gtaaatggtt
    28201 tcgcctgctt taatggtggt agtcatgggg gtggtatcaa tcgtataagg cgccggtact
    28261 gatttttcgg taatggttac ttttgtacca tgaggaatct catccaatgt agcaatgcct
    28321 tctttgtcag tagtcacgtc ttttgcaggt aaagtttttc caaagtctaa atggaaaacc
    28381 gttcctggta caacatcacc cgattcttta tcgacttttt tgatctttaa agaaccctcc
    28441 gtttccacat caattttgat tgtgtaggta ttaggtttat caatcgcccc ggccattaaa
    28501 gtctgttggc cagccttttt ataggcgaca ggtgttccgg taccagcaga tttcttaagc
    28561 gtcagcacac cactttcttt tgaattggca tttggggtga ttatcaactg attaccattc
    28621 acacgataat caatgtttgc ggcattttcc acaacctcat cgaactctga caaattcaga
    28681 ttattcgtat ccgtcacagt agtcgattgc cctaatactg ttttagccgt actattatgg
    28741 aaggttggtt tcttctgata atcgtcaatg gcttgattta tttttgcctc aatggctgcg
    28801 atatttacgg cactaccatc tgatcgtttt attgagtgaa gggtataccc attaacttct
    28861 tgccagatca tcttctgtgc cacaatatga gtatctgcat ctgtacctac ttttttccat
    28921 aaaacggaaa ctaatttggc tttatcggac atatctggta acgggttttt ctcatagcca
    28981 ggagtgaact cattgtaaat cgggactcct ggttcaatac agaaaatatc ttgtttaaag
    29041 ccgttgtaca ctgcataatg cggaacagtg acttctgaca ttggacgacc atcactaaaa
    29101 gttccttcaa cagcatattg atgtgctaaa tctagttcta cggttaccgt ttgtggatgg
    29161 gtaatttcat cagcaaaagt aatcgtagga gcaattgctc caacaattgc ttgaacagcc
    29221 aaactcaaaa tagttaatac acataatttc tttttttgtt ttttatacat gaaatgttct
    29281 cctttcaaat taagcacaaa aaaataagat gctcgcttga acatctttgt ttataaagtc
    29341 tttttaaaat ttttattgac tgaatagaat ggatttgaga atgaatgagc ctaaaaattt
    29401 aatctaaaat acgaaatata ctgcctttaa attagaacac attttagaac acaaacattt
    29461 caaatttaat caaatataat aaaatatatt tacgggaaaa cctttataaa tcaaggtttt
    29521 tcaaacttat tcaatgatac gaagaaacca ggtcttaaga aagcacgtaa agcttcacag
    29581 ttttcaaaac gttaatcgtt gttataccaa cgatttcaga cactttctca ttgcgagaag
    29641 gtgtcttttt ttcgttctgg taatgagaaa ggtaatgaca atattataaa ccagtgtaag
    29701 caatgaattt ttctaacgca tttcgttgag ctttttcagt aacatgagaa taagtgttaa
    29761 gggtagtttc agcattttta tgacccaatc gatcttgtgt ttctttgata cttgctccac
    29821 cttctagtag aagagtagcg tgagtgtgcc ggaatccatg aatagtaacg tgcttaaatt
    29881 tatatttctt ctctaaacgt ttcaatatat tatttgaata gtccgcatgc aaaggttgat
    29941 taatgtcacc gtctgcattt acataggtaa agataagttg attttttgta tttgtattta
    30001 tgccaaactg caaaagctct cgagcttgtt cttttttcca gatttttaac atttcaatag
    30061 tttgtgcatc aattggtaat attcgttcgg aagatatatt ttttgtattt ttttgaatcc
    30121 tgacagtttt tcttcttgca ttcttctctt catttcgaat agttgcatag ctctttctaa
    30181 catacattag ctgttcttca aaataaatgt cagaccaatg aagagcagct agctctcctt
    30241 tacgtatacc agtagtaatc aaaagctgga agattaaaat atgttttaaa ggctcttctt
    30301 tattgagaat atcccaaaaa gcttttagtt cttctaatga aaagtaattt tcattttctt
    30361 tcttttcccg ctttggtaga tttgtatttg ccataggatt tcggtgtaat agctctgcaa
    30421 aaatggcata ctcaaatatt tgagagctgt aagacttaag ctgtttgata ttcttgaatt
    30481 ttaaagccca ttcattgact gcattctgac agtgaacagg agtaatctta gaaatggact
    30541 tattcccgaa ttttggtagt atatgcaagc ggaaaaggtc ttttgtacgt tgatatgtcg
    30601 tttcttgtac acgattccta taagaaggca accattcttc atacactgtt ataaacaagt
    30661 cattagaagg cttatgatag ccggtatctt caatctcgac cttttttctt gcttcgtgta
    30721 acttggcttc tttttcagtt ttaaaccctt tttttacata gtattcttgc tttccatttt
    30781 catctttacg aacatacaag cggacactat atcgtgcacc gctttttgtt gtgtactttt
    30841 gtattgacat aactaatctc tccaatctga taacttggcg agttggctga ttgaagaaat
    30901 agaaaatgag aggagaaaaa taatggatgc aggaattaca gtaatgttag ataaacaaca
    30961 aacgaatcaa ttgaaaaatt atgtttttga aatgacaaaa gaggctattg atcaagcaag
    31021 aattgatgcc ggattagaaa gaccatttct aaaaggaaaa gagatggcta aatatttgaa
    31081 tgtatcatat acaacttttt tgaaatttaa aagaatggga ttgccagtta ttttattaga
    31141 aaaaatggag ttgttttcaa aagaagaatg caaaaaatgg atattgagtc atcaaattta
    31201 aaaaacacct cgttttttaa atttgatgaa gtagaaaaaa ttaaaaagag tgttcccgtc
    31261 agtttgcaga tcgggggaac actcaataat aaagttctga gaaaacttta tttgaatcta
    31321 ttatagcaaa taaaatcaga agcaggtgtg acaatgaatg aaaaagaagt tgaaagctta
    31381 gttgaaaagc aaggctattc taattctgat atatatgcag aattatacta tcaatatcct
    31441 atttttttaa acgtaaatga aaaatataag aaaatgaaac taagttctaa gttagtttat
    31501 atgttactaa aagatagaaa tagactttca ttgaaaaata acttgtatga tgagaatcaa
    31561 aaaatttttt tgatatttga aatcaaaaat ctcgtagaaa tttcgggaat gtcaaaaaat
    31621 acaatcttat cagcactaga tgaacttcaa aaagttaaac ttcttgtgaa aagaaaaaat
    31681 ggcttcaata aaagtaaaat gcaaatgttc ccaaattttt attatttgtt aaagcctgtt
    31741 ctgtgcaaag atgatgttta taaaattaat aagataaccc atcaagaaaa tagacaaaaa
    31801 aacttgatag ttcaaaattt gaactatgga acaaatcaat attctgttga tagttcaaaa
    31861 tttgaactat gcgacacatc aatgaaaaca agagacaaaa acaattttgc tgatagttca
    31921 aaaaatgaac tagaattata taagtcttat aaagatagta aagatattaa agaatcacat
    31981 aatgatgatc ttcttgaatc actttcaact aaaaataata aattagatga agaattgatc
    32041 gaaacattta ttgaagaaaa tcttcttaaa caaatatggg gagaaagtat tgtgaattgc
    32101 ttaaaactgg ctagcaatag tgattatcgt caattcgaca actggtacaa aaaattcaaa
    32161 tatgcgatta gatctgctga aaaagaacaa aaaattcaac tgaaaattat ctatgaaatt
    32221 tgtaataaca agtattttgt agatcatgtt agagaacagc tatcaatgac tttacgtgac
    32281 ttgatacgaa gagcaaaaac agatcatcgt atcaaacaaa aagacgatta tatctttgct
    32341 tctctaaaaa ataaagcgtt agaactaatt gaattgcaaa tcagcgaagg aatagataag
    32401 caataagaat agaattgagt caaatgaaat ggaggggtat ctccacttca tttgagaact
    32461 ttgaatatag gaggggttat tttgattaaa aatcgtttaa tagatcaagt ttggtgtcta
    32521 gatcaaaata cagatagtct cgaattaatc gagactattt gcttcaacac tatagttctc
    32581 gatttaagag ctgaaaatga taattgtgta actcatgtca taattaatca aaaaatggct
    32641 cagaagctct ctgagagcct cagaaaatgg gctaagggag gtaacgatga aaataattga
    32701 aaaaaagatt gccaataaaa aaataaattg gttggttgtt aattttcttg ttctgatagc
    32761 tatgattatt tttacaagta atattgctca tgcgagtatt ttcaattggt ttgatgacac
    32821 aaaaagtcaa acagagtttt tgaataattc tctttataga ccttacctaa aaaagtacaa
    32881 tggagatttc cttagtcagt tcgggatttg gctcggatgg gctgtggtaa aaggcatgtt
    32941 tactgtgaca gattcgattc aaaatatgat tccagatgtg ctggatttgt tcaatttcat
    33001 tgaaagtaca gggctaaata atgtttatca atcagccgta aatacaattg tcattggatt
    33061 aatgatcctt tcattgatgt ttgttggtta caaaatgatt actggaaaag gaacagtgga
    33121 tttaaaatcg gttggaatga acgtagtgat gtctatagca ttaatacttt taatgccaac
    33181 catgatttct agtggtattc aatttgctaa aacattttat aaggactcta ctacgattac
    33241 aaattcagat gacggagtcg cttggtcatt gataaaacaa ggagtaacag atttagcata
    33301 tattaataaa acagatcagt actcaagcat tgataaaacg gaggatcgaa ataatttaac
    33361 acgaaaaaat tttcaacaga cggatctcac acaagtgtta acggataaag tgattgataa
    33421 attggaaaaa gaaaatccaa ttgcggacaa tcttcgttat gaattagttg aaaattctaa
    33481 taatgaattt gttgctacaa agttttctga caacttctta tcaacatttt ctgatagctt
    33541 gaaatcaggc tattaccgat accaagcaaa tctatggggg atttcaattg ggttgacagc
    33601 gttagcaatc gcctatgtgt tcagcgcgtt tgtgatcatt acagcaatac tagaacttgc
    33661 atttaaacgt gtgttaggtg ttcttgtttt tgcaacagat attgagacag gacagcgttc
    33721 aaaagttgtt ctatcagata ttctacaatg ttacttaact gttgggtttc aaggatttgg
    33781 gttatcaatg tttgctatgt ttatcaattt tctgaactca ggacaaggta tctcaacaaa
    33841 tatttttata aaaacaattg cttatatctg tgccgttttt gttttaatca aaggcagtgg
    33901 aacagtcatg agatactttg gtgttgatat tggactcaaa gaaggctatg ggcaacttgc
    33961 ttctgctttt ggaatgggcg caatgctgtt tagaaaagga tcaaatggat ttaatcgtgc
    34021 taaaggaagc ggagaaggag aagatcgaaa accagaaaag aatttcggcg aaactctatc
    34081 aaaaaaagct ggcaaaactg gaagagcttt gggatatgcc catgaacgtg gattatccgg
    34141 tttagcttca gatggagcta caatggctag tgaaagagca acaaaaccat ttaagagtat
    34201 gcgtgatata gctaatgata ccaagaataa gtttaaagaa ggtttagatg atggtacagt
    34261 gtccgctatt aataaaaata gcaaacctat gttagctaaa aataaagaag atgaaactgg
    34321 aaaatatgct gattccgtgc caacacgtct ttcagataga aaagatggtg ctaaagttga
    34381 aaatgccgat agaattatgt ctagttctga acgtatgcgt gaagcaatga aaaataatgc
    34441 tgaatcgaat aataatcctg caagcgcaat tcagcaaaaa gtccaacaag atattgaaga
    34501 acgtaaaaat gctatgcacg gtcaagctaa gtctgctgaa gaattaatta atcagaagcg
    34561 tcatgaagcg aaatacacgc ctgaagcaat gaatcgcgag gaaataatgt taaggaaacg
    34621 tgtagaaggg cggacaggcg ctaatattga cggaaaagaa gcgttgacca aagaaagaat
    34681 tcaagaagct aaaaattcaa atacaggatt agaaaaatta gtcaaagaaa atccacaaaa
    34741 ttctacaagt agtcatggtg gccgatcagt agatgtccga gaaaatattc aaggttcact
    34801 gaatggtcat gttggacgga atgttgatgt aagagagaat ttacaaagtt cttcaaatgc
    34861 tcaaggtact aagacaattg atgttcgaga aaatctgcaa agtagcagta caagtcaacc
    34921 aaaaacagta gatgttcgag aaaatgtaca aaattctaca agtagtcaag gattcaaaac
    34981 agtagatgtt cgggagaaca ttcaaaaaga tagtggagaa atgaaagaaa aaacacaaaa
    35041 aatcaatatc gttgaagaca gaaagtcagc gaaaaaattt gattcaaatc atgaaacagt
    35101 gattattgat agtgaaatcc gtgaaaatga taagggatca aaatcaagac gaagattcac
    35161 ttatgaagac aatgagttat tcagagatac acttaatgat cctaatccac ttttcgataa
    35221 tttattaaga aataaaaggg ggggattgtc atgcaattaa ttctaaagat tagtacgcat
    35281 gatttcttag aacttgacga attatgtgaa tcagtaaaga acgcaacaaa tgaaaaaatc
    35341 acggtcacta attatattga actgatgatt caagaagaat taaagatgaa aaatgaaata
    35401 gagagcaatt tcaaatgttg ctctctagaa aaaaatgtga ggggataatc aatggatgat
    35461 aaacaaaaaa tgtacgaaaa aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat
    35521 aaaaagactt atcttgaact gacattgatt gcaaaacaaa ggggaatttc tagagcagac
    35581 gtttgccgtg agcggttaaa gggactcaaa tatgaaccgc cgttgttagc tgaagatgat
    35641 agggtagttt taaccaaaca gttaaatcaa attggtaaca atttgaatca agcggtaaag
    35701 gcattaaata tcattgctaa atattttaga gaagaatctg aagataaaaa agaaaagagc
    35761 ctacatttta gttttgatgc tagccgttac aacattttta aagatgaact tgcgaatagc
    35821 atagcaaaaa agttgatgat tagttatcca gatgacattt ttttggagtc caatcggagt
    35881 gccccctctt tttttaaatt atcagaagta gaaaaagaat atttcgaata ttggatagaa
    35941 aagcaaacta gaccgggtac gaaagaagag tattcccaaa aagttgatcg gaatttaatt
    36001 cactcaattc agattattgc aagtttaaaa gatgaggtga aacaactttg ggagctacta
    36061 taacatcgaa aggacgcaag aaaaatttat cctcgctatt acaatatgca gctaatcctt
    36121 caaaaaaaga aaaatgtgtt gcagtctcag gaataaattg tagtgatgat agtgaggaag
    36181 cactgcaaga aatgaattta gtacgtacca agatgaataa atctaaagat atagcacaag
    36241 cgtttcatgt tattcattcg tttgataaga gtacttctaa agagttaagt attgataaaa
    36301 tgcacgaaat agctgtcgaa tttgcagaga aagcttttcc aaattcgcaa atcattgttg
    36361 caagtcacaa tgataaagat cattttcata gtcatcttgt cattaataat attaacatgg
    36421 aaacatgtaa aaggttgcgt attgacccta aagatttaga actaatgcga aaaattaacg
    36481 atcaagtcct caatgaacat ggtcttgaat cagtgaataa tgagtactat gaaaacttag
    36541 aaaagagaac caatctttat tcaaacgatc cttctaagat gaaatatgga aaaattaatt
    36601 atcaagagtt tgtgcaaaat gcgatcgaag caacgttgga aaaatgcgaa gtgaattctt
    36661 ttgaagattt ttcggatcat ttggcaaaag aatataacat tgaagtttat caattttcaa
    36721 aaacaaataa caaacttggc tatgttcttt acaaacaagg tgtaaatttc tatgagcgta
    36781 gaggtgagtt aattacttta ggaagagata aacaatctag aaaagaaaaa agtcagtatg
    36841 tcgaacgtac ttttagtgca agaaagttag ggaaaagata ttcatttgaa tctgtttctg
    36901 aacaacttga aaagaattta gaagaatcac atataaaaaa tttttttaat atggaaagag
    36961 agcaagttat gtcgctaatt agtggagtaa attacaaaac tggtgagtta aatgtaccgt
    37021 ttgtggaatt agattccatc gcacaaaagc aatttagcga ccgtgaaggc gatgaaaaat
    37081 tcattaaata tgatgacttc ttgcgagagg tagacaagca ttttgtaaat acaatgtctt
    37141 tagaagatga tcaagaatac acgattaaac ttcatacgga tgtgaatcct caaactttac
    37201 gtagtgcgaa aagtaaaaca atttctatta aaaagactga tcagaaagag ttatttctac
    37261 cggcaaaggg ttttgaaaat tgttctcata caccttctgc taaacagatt cactatcaaa
    37321 tcagtcgcat aatgaatgac gatgtaaaaa tgtatcgtgc taaaaaggat tatgaattca
    37381 cacaacaaat gattgaaaat cagcaaaaaa ggcaacaaaa ttttgaaatg gaattctgaa
    37441 tgggcttgtt agtggacttt ccagtttttt gcctatgaag agttgcttgg aaagaagcag
    37501 tttttaggac atacgccagc tacccatcta cttgccagtc aatccgcccc gctcacgcta
    37561 ggctactttc tggcaagtca gatggcgggt aaatctctga gggagcaagc tccctacaga
    37621 ctccctccca aaaaaagaaa ggtggaagaa aaaaatgaac tatccatatt ttaaagtctc
    37681 ggcttcagaa gaaactaaag aaatattcaa caatttttac aaccaaaaca aaggaatttt
    37741 tggttcaaag gcaaatatgt ttcgtgttat ggtttcaaat ttaccagtgt tagctagtcc
    37801 atcaaataat aaatttaatg atcctgaaag cattaaattt gaacaaaaaa tttcagaact
    37861 agaatcaatg ataagtaatg aagtaattga aaagctggat gacattgatc aaaagttatc
    37921 gtattctcta aaaaacaaat acaaaacgga ggagaaaaaa gatgtttaaa gtttacttcc
    37981 cgtttcagtg gctcgttaaa ggaattcttt tagtgctact aggattgatt ctttttggaa
    38041 gttatctaac aattaaagcc agacattttc cagaagaaaa gccaataaaa attgtaacaa
    38101 aagatttaaa aataggtgaa acttatcagg cagatggggt tgaactaaca tttaaaaata
    38161 ttgaagtgac cgaaaaaaga aatgaatttg cacaacatga tcgtgtatta caagtgacct
    38221 atacacttat aaatgataca gaaaatccga tacgtccaca acaatggtcg attgaaaata
    38281 aggatggtga agaactttat gagtatgata tgatattgga taatccagag tatattgaaa
    38341 gtggcaaaga agaaacagta aattactatt atgaattacc gattgaaagg caggactacc
    38401 aaatcgtagt acgtagagag aatggttaca tagttgggtt gccatttact ttataagttt
    38461 tattttgtta gtagttttta aggtaatatc agttttgtga tttttgaaaa tggaactggg
    38521 agaatggctg aggaaaataa agttgttccg tttgaaacat tagtgataaa taaggaagag
    38581 tttgagcaac tttttcttga tttttttatt caatatgaca tgatttatga ttttagttcg
    38641 tcactagatt tatttgaatt agaagcagaa ataatagaaa acagtcgaga gaagaatggt
    38701 aaaatcatta tttcagtaga aaaattcaat gaattattct cacaaacatt tgaaatagat
    38761 ccagactatt atcaaccaca agaagttttt cgagagttta gaaataagat tattggtttt
    38821 agtcaaacta caaaatgaaa gcttaataaa gcaagtgttg atagaattat tcctcaacgc
    38881 ttgctttatt ttatgggcac ttcataatag aggtatagag aagagaggta ttcattttgg
    38941 atactattac tccaacaaac ttcaaagaaa atataggtaa agtgttgcaa gaagtagttc
    39001 attcaaattc tgaaatagaa attactaggg attctaaaaa tggattgaac gatggcgtca
    39061 ttatgatttc taagagaaaa tgggagttgt tacaagaaga actttattta caacgaacgg
    39121 gtacatgggc ttatgtatct gattta
//
LOCUS       DAFELO010000019        38327 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFELO010000019
VERSION     DAFELO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.019"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            154..384
                     /locus_tag="LP452_001088"
     CDS             154..384
                     /locus_tag="LP452_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL2013828.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            481..1782
                     /gene="murA"
                     /locus_tag="LP452_001089"
     CDS             481..1782
                     /gene="murA"
                     /locus_tag="LP452_001089"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL2013829.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1795..1971
                     /locus_tag="LP452_001090"
     CDS             1795..1971
                     /locus_tag="LP452_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL2013830.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2106..2402
                     /gene="yidD"
                     /locus_tag="LP452_001091"
     CDS             2106..2402
                     /gene="yidD"
                     /locus_tag="LP452_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL2013831.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2586..3053
                     /locus_tag="LP452_001092"
     CDS             2586..3053
                     /locus_tag="LP452_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2013832.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3138..4022
                     /locus_tag="LP452_001093"
     CDS             3138..4022
                     /locus_tag="LP452_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2013833.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4136..4372
                     /locus_tag="LP452_001094"
     CDS             4136..4372
                     /locus_tag="LP452_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL2013834.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4460..4540
                     /locus_tag="LP452_001095"
     tRNA            4460..4540
                     /locus_tag="LP452_001095"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4494..4496,aa:Tyr,seq:gta)
     gene            4547..4618
                     /locus_tag="LP452_001096"
     tRNA            4547..4618
                     /locus_tag="LP452_001096"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4579..4581,aa:Gln,seq:ttg)
     gene            4743..4913
                     /locus_tag="LP452_001097"
     CDS             4743..4913
                     /locus_tag="LP452_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013835.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5133..5840
                     /locus_tag="LP452_001098"
     CDS             5133..5840
                     /locus_tag="LP452_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2013836.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5900..6544)
                     /locus_tag="LP452_001099"
     CDS             complement(5900..6544)
                     /locus_tag="LP452_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL2013837.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6671..6958
                     /locus_tag="LP452_001100"
     CDS             6671..6958
                     /locus_tag="LP452_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013838.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7066..7386
                     /locus_tag="LP452_001101"
     CDS             7066..7386
                     /locus_tag="LP452_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2013839.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7467..8780
                     /locus_tag="LP452_001102"
     CDS             7467..8780
                     /locus_tag="LP452_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL2013840.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8777..9496
                     /locus_tag="LP452_001103"
     CDS             8777..9496
                     /locus_tag="LP452_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL2013841.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9624..10175
                     /gene="raiA"
                     /locus_tag="LP452_001104"
     CDS             9624..10175
                     /gene="raiA"
                     /locus_tag="LP452_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL2013842.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10397..12931
                     /gene="secA"
                     /locus_tag="LP452_001105"
     CDS             10397..12931
                     /gene="secA"
                     /locus_tag="LP452_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL2013843.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13057..14158
                     /gene="prfB"
                     /locus_tag="LP452_001106"
     CDS             join(13057..13128,13130..14158)
                     /gene="prfB"
                     /locus_tag="LP452_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL2013844.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14263..14949
                     /gene="ftsE"
                     /locus_tag="LP452_001107"
     CDS             14263..14949
                     /gene="ftsE"
                     /locus_tag="LP452_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL2013845.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14933..15826
                     /locus_tag="LP452_001108"
     CDS             14933..15826
                     /locus_tag="LP452_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013846.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15978..17426
                     /gene="cls"
                     /locus_tag="LP452_001109"
     CDS             15978..17426
                     /gene="cls"
                     /locus_tag="LP452_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL2013847.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17763..18620
                     /locus_tag="LP452_001110"
     CDS             17763..18620
                     /locus_tag="LP452_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL2013848.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18735..19655
                     /gene="pstC"
                     /locus_tag="LP452_001111"
     CDS             18735..19655
                     /gene="pstC"
                     /locus_tag="LP452_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL2013849.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19655..20539
                     /gene="pstA"
                     /locus_tag="LP452_001112"
     CDS             19655..20539
                     /gene="pstA"
                     /locus_tag="LP452_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL2013850.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20554..21360
                     /gene="pstB"
                     /locus_tag="LP452_001113"
     CDS             20554..21360
                     /gene="pstB"
                     /locus_tag="LP452_001113"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL2013851.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21376..22134
                     /locus_tag="LP452_001114"
     CDS             21376..22134
                     /locus_tag="LP452_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL2013852.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22147..22824
                     /gene="phoU"
                     /locus_tag="LP452_001115"
     CDS             22147..22824
                     /gene="phoU"
                     /locus_tag="LP452_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL2013853.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23062..24093
                     /locus_tag="LP452_001116"
     CDS             23062..24093
                     /locus_tag="LP452_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL2013854.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24090..24857
                     /locus_tag="LP452_001117"
     CDS             24090..24857
                     /locus_tag="LP452_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL2013855.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24957..26525
                     /gene="liaX"
                     /locus_tag="LP452_001118"
     CDS             24957..26525
                     /gene="liaX"
                     /locus_tag="LP452_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL2013856.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26822..27142
                     /locus_tag="LP452_001119"
     CDS             26822..27142
                     /locus_tag="LP452_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL2013857.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27142..27492
                     /locus_tag="LP452_001120"
     CDS             27142..27492
                     /locus_tag="LP452_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL2013858.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27659..28597
                     /locus_tag="LP452_001121"
     CDS             27659..28597
                     /locus_tag="LP452_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL2013859.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28612..29442
                     /locus_tag="LP452_001122"
     CDS             28612..29442
                     /locus_tag="LP452_001122"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL2013860.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29486..30511
                     /locus_tag="LP452_001123"
     CDS             29486..30511
                     /locus_tag="LP452_001123"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL2013861.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30526..31464
                     /gene="galU"
                     /locus_tag="LP452_001124"
     CDS             30526..31464
                     /gene="galU"
                     /locus_tag="LP452_001124"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL2013862.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31589..32029
                     /locus_tag="LP452_001125"
     CDS             31589..32029
                     /locus_tag="LP452_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL2013863.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32032..32634
                     /locus_tag="LP452_001126"
     CDS             32032..32634
                     /locus_tag="LP452_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2013864.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32697..33800)
                     /locus_tag="LP452_001127"
     CDS             complement(32697..33800)
                     /locus_tag="LP452_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL2013865.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34010..35011
                     /gene="ccpA"
                     /locus_tag="LP452_001128"
     CDS             34010..35011
                     /gene="ccpA"
                     /locus_tag="LP452_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL2013866.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35366..35761
                     /locus_tag="LP452_001129"
     CDS             35366..35761
                     /locus_tag="LP452_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013867.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTCSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35761..36012
                     /locus_tag="LP452_001130"
     CDS             35761..36012
                     /locus_tag="LP452_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013868.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36055..36450
                     /locus_tag="LP452_001131"
     CDS             36055..36450
                     /locus_tag="LP452_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013869.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36447..37703
                     /locus_tag="LP452_001132"
     CDS             36447..37703
                     /locus_tag="LP452_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL2013870.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37715..38038
                     /locus_tag="LP452_001133"
     CDS             37715..38038
                     /locus_tag="LP452_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013871.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38031..38297
                     /locus_tag="LP452_001134"
     CDS             38031..38297
                     /locus_tag="LP452_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013872.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12859 a         6513 c         8259 g        10696 t
ORIGIN      
        1 cagtcccttt ttttgcatat ttttagcatt tatttcctaa aaaatgtaag gggattccta
       61 tatttttaag tatacttgtt tttcctttta tacattttat ggtattatca ttttattagt
      121 tattataaaa caatcgttag aaaggacgtc attatgcaag tatacggaat tgatgcggtg
      181 cttcgaatcg tgagtcattt ggcgtttatc tacttagcct tctggtcttt acgttcgtta
      241 cgcattgaaa cttttttcag accaatgcat aatacgcaaa ttagaatggc aatcgtactt
      301 ttttcaattt ttttagggta cactgcaagt aattttttct tagaagttat cgctttatgc
      361 cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta ctattttaaa agcaaggatg
      421 ttataataga aaagattttg agtaagtgta tgctcattgg aattttttgg agggaacaac
      481 atggaagaga tcatcgtaag aggtggcaat caattaaatg gaacagtaag aattgaagga
      541 gctaaaaatg ctgttttgcc tattttagct gctagtttgt tagcagaaga aggtattacg
      601 acattagaca acgttccgat tctttctgat gtcttcacta tgaatcaagt gattcgtcac
      661 ttaaacgtag atgtagattt tgatgaacaa aagaatcaag taacaatcga tgcttcaaga
      721 caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa tgcgtgcttc aatcgttgtg
      781 atgggtccgc tacttgccag aaatggacat gcaaaagtag ctatgcctgg tggatgtgcg
      841 attggtaaac gaccaatcga tttgcattta aaaggatttc aagctttagg agcaaaaatt
      901 attcaaaaaa acggctatat cgaagcaatt gcggatgaat tgatcggtaa cacgatttat
      961 ttagatttcc ctagtgtagg tgctactcaa aatatcatga tggctgccgt aaaagcaaaa
     1021 ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa tcgttgattt agcaaacatt
     1081 ttgaataaaa tgggcgctca agtctatgga gctggtactg aaacaatgcg tattgaaggc
     1141 gtggatcatt tacatgcagt caaccactca atcgtacaag atcgtatcga agcaggtacc
     1201 tttatggttg ctgctgcaat gacacaaggc aatgtgctga ttgcagatgc tatctctgaa
     1261 cataaccgcc cattgatttc taaattgatc gaaatgggtg cagaaatcat tgaagaagaa
     1321 ggcggagtcc gtgtcatcgg accaaaacat atcttaccta cagatgtaaa aacgatgcct
     1381 catcctggtt tccctacaga tatgcaagct caaatgacag ctatccaatt agttgcagaa
     1441 ggtacaagtg tagtgactga aacagtgttt gaaaatcgtt tccaacattt agaagagatg
     1501 cgccgtatga acgctcatgt caaaatcgac ggaaatgtag caatcatgga tggaaaccat
     1561 gaattgcaag gtgcagaagt atatgcaaca gacttgcggg cagcagcagc attagtttta
     1621 gctggcttga aagcaaatgg aatcacacgt gtgcgtaact tgaattatct agaccgtgga
     1681 tattataatt tccatatcaa actacagcaa ttaggtgcag acgttgaacg tgtagacatg
     1741 gatcaaactt ctgctgaaaa aacagcacaa acgattgctt aaggaagtgg ctgaatgagt
     1801 aaccaacgat atatcttatt aactttgata aagattttag tggtgatctt attgcttatc
     1861 cttctttttg tggccggaac gatgatcgga tatggtgtca tcggtggcgg caatccattt
     1921 aaagtattcc agccaagttt gtggattcat attcgagact tttttcattg ataaataaga
     1981 atcacagtgg ttctagtcga tatgaagtga gccatgaagc agaacatgac aaaagaaaac
     2041 agaagggaac gttgacgtta gtcgcgatcc ctttttttag taaaagaaaa gaaggaatct
     2101 aattgatgaa gaagatattg atttatttag ttagaggata tcagcgcttt atttcgcctg
     2161 catttccacc tagctgccgt tattacccaa cctgttccaa ttatatgata aaagcaattc
     2221 aaacccatgg tgcactaaaa gggacggtaa tggggactgc acggatctta cgctgtcatc
     2281 cttttgtcaa aggcggtatc gattatgtac cattgacatt ccaattaacg cgaaataaga
     2341 aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa tacgagtagc actgagcgat
     2401 aaagaaacaa tttggaaaat agcttcttat attttgcaaa attgtgagtt attgccgaag
     2461 tgttggattt tgaacaccgt caaaaaaagg ttgtgaaaaa tacgggtagc actgagcgat
     2521 aaagaaaaag aggaaaatta ttgtcaaagt aacaactccg caactactta tatgggggac
     2581 aaaaaatgat cgaagaacta aacaagaaag atttaccatg gtccttgtta ttagatgcag
     2641 atcctgataa agaaaaagtt caagtatatg tttccagagg gagtggctgg atatggaaag
     2701 aaaaagaaaa aacaataggt gtactgatct atgtggcaag ggaaaaagaa tacgaaatcg
     2761 tcaatgtagc agtagcccca acgtatcaag gaaaggggat tggcggaaaa ttacttgaga
     2821 cagcttttca gaagctttca caactcgctt ccagtcaaac tcagatcatc atacgaaccg
     2881 gcagtactag cagtgcggcg ttacatctct atcaaaaaat gggctatgtg gagattggtc
     2941 gagtcaaaga ctattttatc catcattact ctgagcccat ttttgaaaat ggagatcaat
     3001 taagggatca agtaatttta gcaattcact tacctaaaaa tagctttaac taataaactt
     3061 tcaattcgag aaaatgcttt caaatgctaa atagatacat tacaatagct ttagcaaaca
     3121 aaaaggagaa tatttttatg ggaatattag tttttgattt tggtggatcg gctgtcaaat
     3181 atggttgttg ggatggcaaa gagattaaag gtaagggaca atttgcaacg cctgaatctt
     3241 gggaagaaat gaaagcgcaa ttatttcaag tttataaaaa aatactcgtt tcttttgaag
     3301 tcgaaggaat tggaatcagc tctcctggag tagtgaataa tgagaaacag gtgattgagg
     3361 gaatcagtgc gattccttat attcatggat ttaatatttt ccgagatttg gaaactctgt
     3421 ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc cggaatggct gaattttatg
     3481 aaggtgctgc ccgtgattat caagatgttg cttttgttgt ggcaggaact gggattggcg
     3541 gggcgttgtt cacgaaagga caaatcaata aaggggcaca tctttatggt ggagaatttg
     3601 ggttgatgtt cttaaatggc gataagacat tcagtaaact tggtacagcg gtacagatgg
     3661 cttggcgtta ttgtgaacgc aaaggtctcg ataaaaattc ttataccgga aaagatgtct
     3721 ttgaattagc agaaactgga gatgcaatag caaaagaaga agttgagagt ttttatacgt
     3781 atcttacaaa gggattattc agtattcaat tttcgtttga tccagaagtg atcgtattag
     3841 gcggcggtgt ttctgcaaaa gaagggctca ttgatgagat caaaagccga atgaagaagc
     3901 tgacagaaca attcgatctg catgatttcg aaccgcagat tttattatgt gaatacagaa
     3961 acgatgctaa tcttgtaggt gcagcagcaa actatctcgc tgtacgtcaa ggggagaatt
     4021 aagagcttta gagaaggtgc agcttgcata aaagaagtga atgaaatgaa taggagattt
     4081 ttacctaagg aatgttttat ttgctgcagt atgataaaat atctgagagg tgaaaatgat
     4141 gtcaaaaaaa aataaagatg ttgaagtgcg tatagaagaa gtgaaaaaag atatcaaagg
     4201 taagaattat gaagtcactc agttatttat agggaaaaaa atgattggtg agatcttagc
     4261 ctatgggcca aaagaatatg aaattttctt tggtgaagaa gattttggaa aagaaaaaag
     4321 tcttgaaaat gcgatagaaa cagtgattcg acactggaat ctgcacgaat aaaaaaatat
     4381 caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa ttaggtatta taactaagtc
     4441 aggttgatac aaacctttcg gaggagtagc gaagtggcta aacgcgacgg actgtaaatc
     4501 cgttccttag ggttcagtgg ttcgaatcca ctctcctcca tttcattggg gtatagccaa
     4561 gcggtaaggc aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagta
     4621 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
     4681 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
     4741 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
     4801 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa
     4861 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
     4921 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
     4981 aacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
     5041 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
     5101 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
     5161 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
     5221 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
     5281 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
     5341 ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
     5401 aatcgcaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
     5461 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
     5521 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
     5581 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     5641 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     5701 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     5761 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     5821 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccttacttag aaataaaaga
     5881 gaatcagaat cacctgtttt tatatatgat cagttaccag ttgctcgtga taagaaacag
     5941 gaccctcttc aaattggact tgtccgttca gcagctcaat gatggattct ttaaagtggt
     6001 cagcactttt ttcatctaca gcacagatga cgctaacttg atcagtatat tggatttctt
     6061 ttataaaaaa atgttggagt tcaagataat tctgtaattt tcctagttgc ggatacgtga
     6121 tcatcacaga gatctcctgt tgcaaggtac cttctacgat tccaatgtca gacaatgctt
     6181 gagagacagc atgagtgtat gctcgtatta atccgcctgt gccgagtttc gttcctccaa
     6241 aatatcgagt gactactgca gctacattga tcagttgatt ttttttgagg acttccaaca
     6301 tcggtacgcc agccgttcca cttggttctc catcatcgct gcttcgctgg atttcatttt
     6361 tttctccaat gatgaaggcg ctgcagttat ggttcgcttt ccaatgttct ttttttattt
     6421 cttgaatgaa actttttgct tggtcctctg attctattcg tctaagagaa cagataaagc
     6481 gtgatttttt gatctcaatc tcactttgtc cattttcttt tattgtacga tagcgctcga
     6541 tcatcttgtc acctcttttt ttcattttct ttattcagta taaataaagt tctaggttgt
     6601 ttcaacactg taacggttgc gcaatcgctg tagtatgcga taataaagac aaaggtaggt
     6661 ggatctgggg atgcaggaac gaatgaaaaa gtacgatgca attacgcatt atttgaagaa
     6721 caatggcggc tctcaagtaa cgttgacctt tacccaattt gacgaactgc tgtttcccag
     6781 taacggactt cccaaaacag caagggaaag tacagattgg tgggcaaatg attataaaca
     6841 ccctgaaaaa ggtgcctatg gctggataaa tgcaggctat gaagtagttg tcatcaattt
     6901 ggacaaagaa tatgtcgtgt tcaataaatt agtgaaatct agttggctat ttgattaaat
     6961 aaaccgcgtt tagaaaacgt tgacaaattt ctttcttttt atataataga gtaacagacg
     7021 cgaagaaaac gtttgcgtag tttgttttaa aaaggagaga aaagtatggc taaaaaaaca
     7081 atcatgcttg tatgttcggc tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa
     7141 gctgctgaag caaaaggaat cgactcggat attttcgctg tgtccgcttc tgacgcagat
     7201 aacaatcttg caaataaaga tgtagatgta ctgttattag gacctcaagt tcgctttatg
     7261 aaagctgatt ttgaaaaacg tctggaacca aaaggcattc ctttagacgt catcaatatg
     7321 gctgattatg gtatgatgaa tggcgaaaaa gtgttagatc aagcaatcac gctcataggt
     7381 gaataaatag aacagtataa ctatcgaaca agcgatccaa aaaatagatc gcttgttttt
     7441 tcgtatattt ttttagggcg aataatatga atcatttttg gggtagaagg cttctgacga
     7501 gagatattaa agcaatagac tgtgaggagg taaatttacc aaattatgag aagatcgcag
     7561 cagtggaacg tttgggcaac tggattcttt gtcatcgctg tggaatgagg acgtccgtca
     7621 ttgatggaca gcttgcagat tacggttatt tttgtacaca ttgtctatat ttagggcgat
     7681 gcgacactca acaagattta tatttgtttg atcagcctga agcaaaacct cgagaagtcg
     7741 tctttttttg gacaggacag cttactgaaa aacaaacgga aatcgcaaag agaatacttt
     7801 gccattcaga aaaaagacat catcttattt gggctgtaac aggtgcaggg aaaacagaga
     7861 tgctttatcc tattttagtg gaaactttaa aagctggagg aagagtagcg atttgtacac
     7921 cgcgagtcga tgtttgtaat gaattgtttt tacgctatcg ttatgttttt ccagaagaaa
     7981 aaatcacgtt gctccatggg aatacagcaa cggcttatac ctttacttct tttgttatct
     8041 gtacgattca tcagctatac cgtttttatc ggtcctttga tttaatagtg atcgatgaga
     8101 tcgatgcgtt tccttataac ggggatgcag gattagctca tgctgtgcaa acagcgatta
     8161 aaccagacgg acgattcatc tacttaagtg ccacacctga taaaaagtta ctaaaggaaa
     8221 tcaagcagac gtttgacatc cacaaacttg ctttgcgctt tcatcggcgt ttacttcctg
     8281 aacccgtctt ggttttcatg aataattggc gacaaaaatg caagaacaag aagaagataa
     8341 aatctttggt gcggatcatt catcaattaa ttaaagaaaa ccatatcctt ttattttgtc
     8401 caagtatcac gatgatgcat cgtttgaaag ccacacttca agaggctctt ccgagatatg
     8461 agatcacaga tgtttcttca agagacaatc agcgggcgga aaaagtccag aaaatgcgag
     8521 aagcaaaata tgacatactg ttaaccacga tgatccttga acgtggagtc acatttgaaa
     8581 gaatatccgt tatcgtttta ggagcggatc atccggtttt tactaaatct tctttggtac
     8641 aaatcgcagg aagagccgat cgtaaagggc cttatacgaa cagtcaagta tactttttct
     8701 atgaagaaaa aactgcagct attcgaaaag cttgtcagga aatcaaagaa atgaatgaag
     8761 aagggaagaa gatgctgtga ggtgcagttg ctgcagtaaa gatatcgtcc aaaatttaac
     8821 attcagcgag attattggtg tttcgaaata cagattacct cagacttgtt cagtttgtcg
     8881 cgcacaattt catttattgg aaacaagagg ctgttctggc tgtcaaaaac caatccattt
     8941 caatctagag agtacgaaaa agaaagtaaa gtctattccc gtttactgca gtgactgtca
     9001 agaatggaag aagaaatatc cgacctatga ttttcagcac cgtgcctttt ttgtgtacga
     9061 tgaaaatatg aaggcctggc tcaatcaata caagtttatg ggagatatcc gactagcagg
     9121 cacctttgca tataaatgga cgcagataaa aaaagaatac ccgacataca tctattgtcc
     9181 aataccttta tctgaaaaaa ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt
     9241 gaaagcagca agaattcctt ttcagatgct actcaaaaga gagaagcatc tgctggctca
     9301 agcaaaaaac acgcgaaagg aaagattgga gatgcctcaa ccttttgaac ttgcagtcac
     9361 cttttctaag atacaaaata aaaaaatatt gattattgat gatgtctata caacaggaca
     9421 gactcttttc cacgcagcag attgtttgca ggtcgcacag ccgaaaatca tccgaaccat
     9481 ttctttagca agatgaatta gatgtggaaa tttgatagcg atttaatttg caaataacct
     9541 agagttcgat ataatagaat taagaagagg gaaagagtgg tccttctcct tcttgattgt
     9601 ggtagacgaa aggggtaatt tttatgttta gatataatgt acgcggcgaa aatatcgaag
     9661 taactgaagc gattcgcgac tacgttgaga aaaaagtagg caaactagaa cgctatttca
     9721 gtgacgcacc cgatgcaacg gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa
     9781 aagtggaagt aacgatccca ttaccttatc ttgtattacg tgccgaagaa acttctccag
     9841 atttatacgc aagtatcgat ttggttgttg acaaattgga acgccaaatc cgcaaattca
     9901 aaacaaaaat taatcgtaaa tcccgtgaaa cgggcatgaa tactgcagat gctactgctt
     9961 tattcaatga agaggacgaa aacgaaaatg attctgaatt agatatcgta cgtacgaaac
    10021 gcctttcatt gaaaccaatg gatagtgaag aagcagtctt acaaatgaac atgctgggac
    10081 ataacttctt tatttttgaa gatgcagaaa caaatggtac aagtatcgtt taccgccgta
    10141 aagatggaaa atacggactg attgaaacag actgatcagc cttacttaaa taacaacgaa
    10201 aacttgcagc agctactgac agctgccgca agtttttttg agtttcctcc tcttttagat
    10261 gaaatatgaa taaattatga agttcttact ttaatacgct taagatattt caattatcta
    10321 taactaatga taaaatgaaa agtaaagtct acatagtctg gataatacac agaaggaaaa
    10381 ggagattaca caacgcatgg ccaatttgat tcgttcactt atcgaaaacg ataagaaaga
    10441 actaaaacgt ttagatagca ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc
    10501 attaactgat gaacaattac aggcaaaaac tgctgaattc aagggcagat accaaaaagg
    10561 agaaacatta gatcaattac ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg
    10621 tgtattgggg ttatatccac accacgtaca attgatgggt ggaatcgtgc ttcacgatgg
    10681 aaatatccct gaaatgcgta ctggtgaagg taaaaccttg accgcaacga tgccagtata
    10741 tctaaatgct ctgactggcg aaggggtaca cgtagttacc gtcaatgaat atttagcaac
    10801 ccgtgactcg acggaaatgg gcgaacttta taatttctta ggattaacag tggggttgaa
    10861 tatcaactca aaatcttcgg atgaaaaacg cgaggcttat aattgtgata tcacttatag
    10921 tacgaataat gaattaggat ttgactactt gagagacaat atggtcgttt accgccatca
    10981 aatggtacaa cgtccgctga attatgccat tgtcgatgaa gttgactcaa tcttgattga
    11041 tgaagcgaga acgccgctga tcatttcagg gcaagcagaa aaatccacag ctttatatac
    11101 aagagcagac aacttcgtga aacgtttgaa agaagaagaa gattataaaa tcgatgtaca
    11161 atcaaaaaca atcagtttaa ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt
    11221 ggataactta tacgacattg aaaatactgc attgacccat cacttggatc aagcattgag
    11281 agccaactat atcatgcttt tagatatcga ttatgttgtc caagacggaa aagtactgat
    11341 tgttgaccag ttcactggtc ggatcatgga tggacgccgt tattcagatg gacttcacca
    11401 agcaatcgaa gctaaagaag gcgtagagat cgaagacgaa acgaaaacca tggcgacgat
    11461 cactttccag aactatttcc gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa
    11521 aacagaagaa gaagaattca gagaaattta taacatccaa gtataccaga ttccgacaaa
    11581 tcgtccggtt atccgtgatg atcgtccaga tttgctttac ccaacattac aaagcaagtt
    11641 ccacgctgtc gtgcaagata tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg
    11701 aactgttgcg gttgaaacat ctgagttatt atcagatatg ctgaatcaag aacgtattcc
    11761 tcatgaagtg ctgaatgcga aaaatcactt taaagaagca gagatcatca tgaatgctgg
    11821 tcaaaaaggt gctgtgacga ttgctaccaa catggctggt cggggaacag atatcaagtt
    11881 aggacttggg gtaagagaac ttggtggtct agcagttatc gggacagaac gacatgaatc
    11941 tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc
    12001 acagttctac ttgtcattgg aagatgattt gatgaaacga ttcggttcag aacgaatcaa
    12061 atctttctta gatcggatga agatcggtga ggaagatgct gttatccaaa gcaagatgct
    12121 gacgaaacaa gtagaatctg cacaaaaacg tgtagaagga aataactatg acacacgtaa
    12181 aaacgtctta caatacgatg atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg
    12241 acaagaagta atcatggaag aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg
    12301 gacaatcagt cgtgtagtag atgcgcatac acaattagag aaagaaaatt ggaatttgga
    12361 aagtatcatc gattttgccg gcagtactct tgtccatgaa gatagtatta ctcttgcaga
    12421 tatcgaagga aaaacagctc aggagatcaa agagtatcta aacgaacgag cacaagaagt
    12481 atttaatacc aaagcggctc aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt
    12541 cgtgatcttg cgtgttgtgg actctaaatg gacagaccat attgatgcaa tggatcaatt
    12601 acgtcagtca atcggcttac gtgcttatgg acagaacaat ccattagtcg aatatcaaac
    12661 agaaggctat aagatgtttg aagatatggt aggcgccatc gagtatgaag tcactcgaat
    12721 ctttatgaaa gctgagatcc gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc
    12781 ttctcactca gaagacggag atgccaaaga aaatagtaat actagcgcga aaaaacagcc
    12841 aatccatgtg aacaaggtcg gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata
    12901 taagaactgt catggaaaaa atgcaaacta atttctgaca gcaggattgg tgagacaaat
    12961 tcttttttta agaattcgtc tcaccttctt tctgttattt gctgattctg ttagactaga
    13021 tataatgaaa aaagtatagt gaaagaagga aaaaacatgg aaatatccga aattcgaaat
    13081 ctcctgacag aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca
    13141 gctggaagaa gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga
    13201 tagcgaagct gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca
    13261 attccattct cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga
    13321 agaacctgat gcagccatgc aagaagaagc agaaacacgt atcaatgcgc tagatgaaaa
    13381 aatgaagaca tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat
    13441 cgttgaattg catccagggg ctggcggaac ggagtcccaa gattggggca gtatgctttt
    13501 acgtatgtat acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca
    13561 agcaggtgat gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt
    13621 tggttatctg aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc
    13681 agcgaaacga cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac
    13741 gatcgatata gaaatcaatc cagatgattt gaaaatcgat acgtaccgag caagtggtgc
    13801 aggtggtcag cacatcaata aaacagaatc tgctgtacgg atcactcact tgcctacagg
    13861 aacagtagta gctagtcagg cacaacgttc acaattgaaa aaccgtgagc aagcgatggg
    13921 gatgttaaaa gctaagcttt atcaattaga agtggaaaag aaagaacaag aagcagctgc
    13981 acttcgagga gaacaaaaag aaatcggctg gggttcacag atccgttctt atgttttcca
    14041 tccatattct atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagcagt
    14101 tatggacggt gaccttgatt tatttattga cggctattta aaaatgcatc tacaataaga
    14161 acccattgtt aaattgaaat aaaattgtaa gattttgcaa gcatgttgaa acattgccat
    14221 gctataatag ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat
    14281 gtaatgaaga agtactcaaa tggtacgacc gctatccgca atctctcaat tgtcatcgaa
    14341 caaggagagt ttgtctatgt ggttggtccg tctggagccg gtaaatctac attcatcaaa
    14401 ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta
    14461 atagcaatca aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa
    14521 gactataaat tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt
    14581 atcggcagaa aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt
    14641 ttgaaacaca aagttcgagt ctttcctagc gaattatccg gtggggaaca gcaacgtgtc
    14701 tctattgctc gggcaatagt gaatacgcct aaagtactta tcgcggatga accgacagga
    14761 aatctggatc cagaaaactc atgggaaatc atgaaattat tagatcggat caatgcccaa
    14821 ggaactactg tcgtgatggc tacacataac agtacgatcg taaatacgat tcggcaccga
    14881 gtaattgcta tcgaaaacgg ccgcattatt cgagatcaag cggaagggga atatggatac
    14941 gatgattaga acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg
    15001 ctggatgaca gttgcttcag tcagtgcggt aacgatcaca ttgactttag tcggtgtatt
    15061 catggcagtt atcatgaacg caactaaatt agctcaagat atagaaggaa acgttgatgt
    15121 ctctgtattc gtagatatcg gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt
    15181 gaatgactta gatcatgtta aaaaagttga attttcaagc aaagaacagg aattgaaaaa
    15241 aatccaaaac gaaatgggtg attcctggaa attgtttgaa ggtgacaata acccgttata
    15301 tgatgtgtat gtagtaagtg ctactgatcc ttcttataca aaacaagtag cagaagaagc
    15361 agcagatttg aagaatgttt ctcgttctga ttacggtgga gcttcctcag atcggatttt
    15421 ccaaatcgct ggtgcagttc ggacatgggg attagttgca gcggcattgc tgctgttcgt
    15481 agcgatgttt ctcatttcga acacgattcg gatcacgatt ttgtctagac agcgtgaaat
    15541 tcaaatcatg cgtttagtcg gtgcaaaaaa tggatatatt cgctggccgt ttttccttga
    15601 aggtggctgg atcggtctga tcggtgcgat tcttccgatt ttgatcatca catttggtta
    15661 tgcatggttt ttccaattga tcaatccatc attattacgt tcccattatt cattgattca
    15721 tccacaaaat atcgtttgga aaatcaatct tttaatggtc ggcattggtg tgatcattgg
    15781 ttcacttggc tcgatcattt ctatgagacg tttcttgaaa ttctaatacc acaatataaa
    15841 taaaatgaga aagttggaca gtttcgttcc agctttcttt ttttattggc taggagaata
    15901 gaaggaagcg gcttttttcc ctttacttta aaaaacaact tgggtattct taagttggta
    15961 gaagaagagg tgaagccgtg aaaattatcg ttgacaattt ttttacgatc cttttgatta
    16021 tgaatatttt gctttccttt atcatcgttt ttcgagaaag aaaggaaact gctcaaacat
    16081 gggcatgggt gctcgttttg atgttcattc cagttgtagg atttatttta tatatttttt
    16141 taggaagagg gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta
    16201 agaacctgga aattgaagaa gacaaacagg cattgatgcg agggctttat cctcatccgc
    16261 ctactggaca agtagatgtg aaacaattga tctatatgct cacggtattt gaaagtacgc
    16321 tctatacgac taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc
    16381 tgattgaaga tatcaagcag gcagaagacc atatccactt ccagtattat atttatcgca
    16441 gtgatgcatt aggtgaagaa gttcgagatg cattgacaga ggcggcaaga cgagggggaa
    16501 aagtcagagt ccttcttgat gcatggggtt ccacacaagt ttcttccagc ttttttcaga
    16561 atttgaaaaa agcaggcgga gaagtcgcat ttttctttcc tctatttgtt ccgtatatta
    16621 atcctcggat caattatcgc aatcatcgta aaatcgtagt tattgatggg aaaatcggat
    16681 atacaggtgg gttcaatgtt ggaaatgaat atctaggatt ggtaaagaaa tttggttatt
    16741 ggcgagacaa tcatttgcgt atttacggag aagcggtgta taccttgcaa aaccgttttt
    16801 tgatggactg gaattctcag catacaaaag aagtaggcta ttctcccaaa ttttttccgt
    16861 ccattcactc tactggtgat atagctgttc aaatcgtgac aagcggacca gataccgaac
    16921 atgaacaaat caaaatgact tatttgaaaa tgatttcgtt agccaaacgg gagatattga
    16981 tccaaacgcc ttattacata ccggatggat cgattcatga agcactgaag ttggctcttc
    17041 tttcaggcgt aaaagttcat atccaaatac ctaacaaacc agatcatctt ctcgtttact
    17101 gggcaacgta ttctttcgct gcagagctaa tcgagtatgg agcaaggatc gaaacatatg
    17161 agaatggatt tattcatgca aagacgatga ttatcgatgg tggcattgtt tcagttggct
    17221 cagcaaatat cgatgttcgt tcatttcgct tggactttga ggtcaataca ctggtttatg
    17281 atgaacgaat ggcttcacgt gtaagaaaag ctttttttga agactcgaaa atttctacgc
    17341 atctcacaaa agaaatgtat gaaaaccgag gaataatcat caagatgaaa gaaggattag
    17401 caagattgat ttcgccgctc ctttaaaaca taaaaaggac atgagaacca tcaacagatg
    17461 gagatctcgt gtccttttct aacggggcca tttctgagag tacgtcagaa atggagaaag
    17521 cgtctcgcat gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa
    17581 taagctaata tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt
    17641 taatgagata gtttttttta caattatgag aatttatttt gttatttaat gattttgatt
    17701 cgttttcatc aagtaatgaa aatgataaaa taaacatact ttaggattaa aggagatttc
    17761 taatgaaaaa acttaaatta ttcttaccaa taataggatt agctattctt ttatcaggct
    17821 gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct gctttacagc
    17881 ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg
    17941 tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca gtcgacatcg
    18001 gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca ttagtcgatc
    18061 atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc gtaaaagaca
    18121 tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg agtgaagtcg
    18181 gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga acccgtagca
    18241 cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa
    18301 gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat gttgcttttt
    18361 cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg acagatgaaa
    18421 atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca agagatcatc
    18481 caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta caagataaca
    18541 tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt gattggcaag
    18601 gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat
    18661 gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta
    18721 agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa
    18781 aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg
    18841 tattatctat tttttatttt gtagctagta aaggattggc cacctttttt actgataaga
    18901 tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc ggagcaaatg
    18961 ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg acctttttat
    19021 ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg gagatctctc
    19081 ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt attccttctg
    19141 tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt atttttggcg
    19201 ggactgggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc ttgccgactg
    19261 tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg gaagcttcat
    19321 tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc
    19381 ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa gccttagcta
    19441 tccaaatggt catcggtaat gcagcattga tgccaacagg tcttactact cctgcttcta
    19501 cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca gttgaaaata
    19561 atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga
    19621 tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat
    19681 tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag cagctttact
    19741 actttatatc ttagttcggg gaataccgca catttcatgg tccttcttga ccgaaccttc
    19801 caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga tttatttgct
    19861 tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct atctttcaga
    19921 atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa tcttgagctc
    19981 gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta
    20041 tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc ctcttttgac
    20101 tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag caggattggc
    20161 cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag cgacaccagg
    20221 gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag ctgcattgat
    20281 ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc cattaagtgt
    20341 ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata tttggaagat
    20401 caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt
    20461 gattattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg
    20521 aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg gaatgaaaca
    20581 aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga tctacatgtc
    20641 tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa aaataagatc
    20701 actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc gttgaatcga
    20761 atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca aggcgtagat
    20821 attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat ggtttttcaa
    20881 cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt gaaacagcac
    20941 ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagctgcc
    21001 ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc tggcgggcaa
    21061 cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac
    21121 gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac acttgtaaac
    21181 ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc agctcgtatc
    21241 agcgactata ctgccttttt ctatttagga aaagtaatgg aatatgacaa aactaacaag
    21301 atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa
    21361 gacggaggcc tcaacatgac aaaagagatt atttcatcta aagatcttca tctgtattat
    21421 ggaaagaaag aagcactaaa agggatcgat ctaagcttca atcaagggga aatcactgcg
    21481 atgatcggtc cttctggttg cggaaaatcc acttatctgc gttctttgaa ccggatgaat
    21541 gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat
    21601 ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt ccagcagcca
    21661 aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa
    21721 aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg
    21781 gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg gcagcagcag
    21841 cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt ggatgaacca
    21901 acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga
    21961 gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat
    22021 aagacggcat tctttttaga tggtcacttg atcgaataca atgatacgaa gaagatattc
    22081 atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga
    22141 aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac
    22201 gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata catcaaacac
    22261 gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa catggaaaca
    22321 cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac aactgatttg
    22381 cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc cgatcatgcg
    22441 gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa
    22501 aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt cctagttgct
    22561 tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca aagagtcaat
    22621 gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca
    22681 gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat cggagactat
    22741 gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgagttaaat
    22801 acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag
    22861 aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa
    22921 acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt
    22981 ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc
    23041 atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt
    23101 tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc
    23161 gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac
    23221 acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa
    23281 acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag
    23341 ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc
    23401 atgttgctta ttggtggaaa acagggtcta tttgctttag gaagtctcat catcaatatc
    23461 gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat
    23521 atatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg
    23581 aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt
    23641 atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat
    23701 tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga
    23761 ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa
    23821 ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt
    23881 ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgtttctt
    23941 ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa
    24001 tttttacgcg tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc
    24061 ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt
    24121 tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat
    24181 tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac
    24241 tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg
    24301 gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc
    24361 tactattgct tcctatcact gattttttag caatccaagg ttttacatcg gtagaactag
    24421 aagaattatc tggcttagat aagactttag cgattgattt tagattactt acgagatcgt
    24481 tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga
    24541 cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt
    24601 tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg
    24661 gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga
    24721 tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac
    24781 tacttgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac
    24841 aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc
    24901 ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga
    24961 aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct
    25021 tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag
    25081 acaaggtaac tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg
    25141 aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc
    25201 aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc
    25261 gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg
    25321 aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag
    25381 cacagcgtca gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt
    25441 tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg
    25501 agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa
    25561 cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat
    25621 tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag
    25681 atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg
    25741 aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa
    25801 cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg
    25861 ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct
    25921 ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag
    25981 tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag
    26041 ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa
    26101 cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag
    26161 attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt
    26221 ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag
    26281 tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg
    26341 aaggacatgc taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag
    26401 ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg
    26461 aaatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata
    26521 aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat
    26581 agcttcccct attttacaaa attgtgcctt attgccgata gtctatctct tgaacaccgt
    26641 ttaccaagag attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa
    26701 agctcgtcac gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg
    26761 attaagaaga aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga
    26821 catgatgaaa aaaaagttga caaaatctaa taaaaatgtt gttttgacag ggactttagc
    26881 aggaatagga gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct
    26941 gagcttagtt gcttttggaa gtccaattat tttatacatt ttattagctt tgattatccc
    27001 atctgcgcgt tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa
    27061 ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
    27121 tgacgaatgg agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca
    27181 ttcatatcat tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc
    27241 gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata
    27301 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
    27361 attcttaaaa tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt
    27421 gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg taattgtcag cgaacataac
    27481 agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag
    27541 gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact
    27601 tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat
    27661 gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg
    27721 cgacgagaaa agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga
    27781 gttgactggt tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga
    27841 aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg
    27901 tagtgaagat atgcccgcat ttattatttc aagaggatta ccagctccag aagaaatgat
    27961 ccaggcagca aatgaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt
    28021 attaggagaa ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg
    28081 tgttttagtg gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa
    28141 aagtgaaaca gcgttggagt tgatcaaacg cggccatcga ttgatcgcag acgatcgagt
    28201 agatgtttat cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca
    28261 tctgattgaa atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc
    28321 ggtacgtggc tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa
    28381 gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc
    28441 acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc
    28501 gatgaatttc cgtgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt
    28561 aagtcgcttg attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca
    28621 caaatcaatc gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc
    28681 atcgtatcag gtatcattat cgctatgtgg cttagcagca gagaggctgt tcgggtagga
    28741 ttaaaagcag atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggc
    28801 gctcgcttat attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata
    28861 ttcaatatcc gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg
    28921 ctgtattttt ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca
    28981 ccaagtgtat tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca
    29041 tatggaccag acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt
    29101 gataatatgt atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg
    29161 agtttgattg gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa
    29221 ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga
    29281 ttacgtacag acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct
    29341 gtgttatttg tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg
    29401 gattatgacc gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa
    29461 tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc
    29521 ttggggtact gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg
    29581 acatcatgct tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc
    29641 agatcattat ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga
    29701 cgcagatgcg gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact
    29761 agtgcctaaa ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg
    29821 aacacataaa aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc
    29881 tgttgttgtt ctttctggac caagccatgc agaagaagta gcaaaacatg acatcactac
    29941 gatcacagct gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa
    30001 taattatttt cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt
    30061 gaaaaatatt attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc
    30121 aaaggcagca atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg
    30181 tgccaacccg ctgaccttta tcggattaag cggaataggc gatctagtcg ttacttgcac
    30241 aagtgttcat tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga
    30301 agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat
    30361 ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct
    30421 gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atggaaaaaa
    30481 agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa agttaaaaaa
    30541 gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa agctatggca
    30601 aaggaaatgt tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg
    30661 aaatcaggta tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat
    30721 cactttgatt caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta
    30781 ttgaaattag tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca
    30841 aagggtttag gacatgccgt tcttcaagca agagccttta ttggaaatga accatttgtc
    30901 gttatgctag gcgatgatat aatggaagac gaagtaccat tgacacagca attgatcgat
    30961 gactatgagc gtacacacgc ttctacgatt gccgttatgc aagttcctca cgatgaaact
    31021 tcaaaatacg gaatcatcaa cccaggggaa gtactggaag atgggttata caatgtcaaa
    31081 aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattgggcgt
    31141 tacttattga ctccagaaat ctttgatgta ttatcagaac aacaaccagg tgctggaaat
    31201 gagatccaac taactgacgc aatcgatacg ttgaataaaa cacaacgcgt atttgcccgt
    31261 aaattcacag gtaaacgtta tgacgttggt gacaaatttg gttttatgaa aacaagtatt
    31321 gaatatggtc tgacacatcc agaagtaggg gcacctttgc gtgaatacat catgtcttta
    31381 ggtaaaacat tagaagcaga aggaagcaag atcaatcata atccaaaagg aataacagaa
    31441 atcaaagaag aaaagaaaaa ataatctttt tttctaacga gcttcagcgg ctaattgaat
    31501 aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag
    31561 aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc
    31621 agcaatcgcc tttgcgatac ttgtcgtttt catcgttcgc gtattgttgc aagtttccaa
    31681 aactttagaa aaagtagacc agactgtcgc agaagcaaat acgacaattg agatcgtcac
    31741 acaagacgta gatcttttat ctagacaagt agaaggcttg ctagtcaaaa gtaatgagct
    31801 gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga
    31861 tttaagtgaa agtgtatcgg aacttaatca ttctagtaaa aacatcgtga cgaaagtcgg
    31921 cggaatcgga aaaacgacag ctaaagcgac tgttgccgga aaagtcggtg gaacagcgat
    31981 gaaatttttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    32041 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    32101 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    32161 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    32221 ggtattgcaa aagaaaaagc gcaaggtctt tctcaacaag caggggatct agcaggcaat
    32281 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gtgaatcaga tgatatcttg
    32341 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    32401 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    32461 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    32521 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    32581 gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca ctaagattac
    32641 tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta ttttcattac
    32701 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc acttgtcaca
    32761 tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    32821 gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga agggaattcg
    32881 tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    32941 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tgggcgaact
    33001 gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc ttggaactca
    33061 gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt ccaaataacg
    33121 ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg tgtaccatga
    33181 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    33241 tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac gccttctttt
    33301 actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc gatcagtttg
    33361 tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt tagatcggtg
    33421 taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac agtcagagca
    33481 ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc ttttccgatg
    33541 atctcccaag gattttcgct gtctaagtag ccacgtacat cgtacgccca gctgctgttt
    33601 tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc cagagggata
    33661 aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta agcgatgtgg
    33721 ccagggtcgc tgatataagc caattctact tggtgttgtg ccatccattg acgtagttca
    33781 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    33841 tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta aaaattttca
    33901 tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta ttttagaaat
    33961 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    34021 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    34081 tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct tggaagttat
    34141 cgatcgcttg gattatcgtc caaatgcagt cgcacgtggc ttggcaagta agaaaacaac
    34201 aacagttggg gtaatcattc ctgatgtcag caatatgttc tttgcctctt tagcacgtgg
    34261 tatcgacgat gtggcaacaa tgtacaaata caatatcatc ttagcaaact cagatggcaa
    34321 cgatcaaaaa gaagtcaatg tattgaataa tttattggct aagcaagtgg acggcgtgat
    34381 ctttatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaacgcc
    34441 tgttgtacta gctggttcca ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    34501 tgtgtcagca acaaaagatg cagtaaataa attagcaaaa agcggcaata aaaaaatcgc
    34561 ttttgtcagc ggtgcgctga ttgatcctat caatggtcaa aaccgtctaa aaggctataa
    34621 agaagcatta agcgaaaaca atctaccata tagcgaagga ttgatttttg aggcacaata
    34681 caactttaaa gatggtctgt ctctagttga ccggatccat aatagcggtg caacagctgt
    34741 ctatgtttca gacgatgaat tagccattgg tatcttggat ggtttattag accgcggcgt
    34801 aaaagtacca gaagaattcg aaatcatcac aagcaacaat tctttactga cagaagtagc
    34861 tcgtcctcgt ttgacaagta tcacacaacc attatacgat ttaggagcgg tatccatgcg
    34921 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    34981 tggaatcgaa gaaaaaggat caactaaata ataaaagatc aatcatttaa aggttgttcc
    35041 aaaagtggtc aacttctgga acaacgttta tacgagaaag ggggttatga caaaattttg
    35101 tcataacccc tttttttttg tgactttaaa aaaagagaaa atggcggaaa caaaccttac
    35161 gtccatcgta tacgacacaa tctgtgcgta gatgctacat tttgttcact actatcaaaa
    35221 ttactttaaa atgaaaacgt gataaaaaag aaagtagaaa agtagataag taactctaaa
    35281 aaatccaaaa aaatccaggc tcgattgcgt acttatctta aaggagaaac aatccatcat
    35341 atataacccg aaaggagact tctatttgcc taagaaaaga cgaaagacca tagtcatcat
    35401 tttgaccatt cttactatag gaatcggagg gaaattttac atggacaaaa gagaaatgca
    35461 aagacagaaa gatttactcg cagtagaaaa acagtccgtc aaagttctga aaaatacctt
    35521 tgcggatatc aaggaagtga agattgaaaa atctgctcga aatgaaatga cgtgttcata
    35581 ccggatagtt attctaatga ctaataaaca agatcaatcg atttattttt cttatagttt
    35641 ttggaaagaa agaaatgaga taggaagtta cggaatagtt gatgaaaaaa agcaaaaaga
    35701 aggaaatacg ctcaataaag ttaaagtaac ttactctaat ggaaatgagg aaagtattta
    35761 atgattactg acaaacaata tagtgaatta agtgatgctg tttactggct agatcctaaa
    35821 catagagact atgtacctga aatgcaagaa aatttaagtt ttaaaattaa cggcactatg
    35881 tataaaatcc taaaaatcaa aaactccttc gacggcatgc aggcgatggc tgtagcgcca
    35941 atcgtccata gcaaattgga gaaaaatttc aaaaataaaa aaattcctgc aaactttcgc
    36001 gtacttaaat aatgaaggtg tcaatactta cataaaaaga aaggaggcac gttcttgtca
    36061 aggaaaataa gaaaactcag cttagccatt ttattaatcg tcatattagg gatcggaggg
    36121 aaagtctata tggacaaaag agaagcacaa aggcagaaag atttactcgc agtagaaaaa
    36181 cagtcagtca aagttctgaa aaataccttt gcggatatca gggaagtgaa ggtagaagaa
    36241 tttaaaaaaa accctgttac cggaagttat ggtgcacttg tgacaatgac aaataatgaa
    36301 gggaaatctg tttattttga ttatagtttt tggaaggaga gaaatgaact tggtggtttt
    36361 ggcattgaga acagagcggt acaaaagaca ggagtcacta taaaaagagt aaaagtaatt
    36421 ttttctaacg gacaggagga gttagtatga caataaacga taagcattac aatgatatta
    36481 gtgagaaggt ttattggcta gatcccaaat atccccgcta caatgaggga tacaaaaaga
    36541 actctgttaa agaatttgct ggtatggaat ttcaaatcct acaaatcaaa gactcattgg
    36601 acggcatgca ggcgatgtct gtagcgccaa tcaagaacgg taaagtggat ctttctcaag
    36661 tagtgattgc ttttgcagga acgaacttaa gtgattggaa agatttggaa acggatgcaa
    36721 gaagtatcat gaatctctac gggaatagac caggagcaag tttttctgaa aaagcaacaa
    36781 caaacaagat tttatccgtt agtggattga aaacagcggg gcaaatcaat tcagcgattg
    36841 catttgctaa tgaagtaaaa aaggaatatg gagaagctgc tatttctact accggccatt
    36901 cattaggtgg attcttagca ttatatgtag cagcagagaa acaatggaaa aatgtcggct
    36961 tcaacgggcc ggacccgtat gatctcttat caccagaagc aaaaaaatgg gtggaagaaa
    37021 atcccggtat gctcaccaat taccgaaatc acgccgattt tatcggtaat tttggtggta
    37081 atggcacagg tgctgaagtt cggatcagta tggaaacagc attcttgaat ctgcttgctt
    37141 cccacagttt aacctcttgg aagtttgatg aaaatggaaa gctgattatt cctgaaaatg
    37201 aaaacaatgt taaagctcga aaacaacaga aagaaaacca agcgacccag ttgttttcct
    37261 atcagttgca agaactgaac aacatacgta aaaaactacg attaagtggc ggccagctga
    37321 cagttaatga acaaatttac ttagatgaca cacaagctag gttagtagtg gaaaagttat
    37381 cggcaaaaat gaaaagcgct atggaagaag ttatccgcat caatcaaaga ggaatttttg
    37441 agctagagaa aaaatggcaa gaaggcttgc ggcaagcacg tgttgtagcc ccttcgttga
    37501 ctgaacagga aatcatagaa gcattagcta gtgtgggcgc cacaaaagaa aacttagtcg
    37561 attctgcgaa agaagtctat ctgctacgaa tcaataaggc aagacgtatg ggggaggcct
    37621 ttgatacatt ggcgaaggag atccaagaca aaatcaatga aatcgtggca cgagatcgag
    37681 aattagcaca gcaattcaat taaaggagaa gtagatgaaa ctagaagaat tggaaagaga
    37741 ttatcgaagt gaacgaagac aattagaagc tcaagaagat gaattgatcc actttaagaa
    37801 tcaaggatta cgattgatgg agcaagagga gcagcgactg catcatgtat taaagaactt
    37861 agctttagat aatgagcctc tgtttcaagc aagacgaagt tattcgaatt atcaggagca
    37921 ttatcaacaa gccctacaag tagaacgacg aaaactctat cagcagcaag atgaactaga
    37981 acgttcctat cgtcaaaaaa tcagacaaat aactatggag aaatcatctt atgaatgaat
    38041 ttttaatgca attactaaaa gaattagaga aaaatctaca tcgatcaaat gtacgttcag
    38101 ttggtatggg gatatctcag caaatttggg cagaaaaacg attgacagat caaaactacc
    38161 aaatgactgc agaagaagct gctcaagaat tttctaaccg acatgtacac ttgaaaggat
    38221 cagtaaaagg gcaatttgca gaaaaactaa ctgaattatt ccaaagacaa gaaaagaaat
    38281 taaaacagtt tcgttagtaa agttatatag aaaaattttg tgaaaat
//
LOCUS       DAFELO010000020        37037 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFELO010000020
VERSION     DAFELO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.020"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            53..1324
                     /locus_tag="LP452_001135"
     CDS             53..1324
                     /locus_tag="LP452_001135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL2013873.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            1321..2616
                     /gene="rho"
                     /locus_tag="LP452_001136"
     CDS             1321..2616
                     /gene="rho"
                     /locus_tag="LP452_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL2013874.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            2715..2978
                     /locus_tag="LP452_001137"
     CDS             2715..2978
                     /locus_tag="LP452_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL2013875.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            3423..3872
                     /locus_tag="LP452_001138"
     CDS             3423..3872
                     /locus_tag="LP452_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2013876.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            3929..5347
                     /locus_tag="LP452_001139"
     CDS             3929..5347
                     /locus_tag="LP452_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2013877.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            5347..6207
                     /locus_tag="LP452_001140"
     CDS             5347..6207
                     /locus_tag="LP452_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL2013878.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            6381..7154
                     /locus_tag="LP452_001141"
     CDS             6381..7154
                     /locus_tag="LP452_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2013879.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            7301..7666
                     /locus_tag="LP452_001142"
     CDS             7301..7666
                     /locus_tag="LP452_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL2013880.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(7814..9022)
                     /locus_tag="LP452_001143"
     CDS             complement(7814..9022)
                     /locus_tag="LP452_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2013881.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(9041..9319)
                     /locus_tag="LP452_001144"
     CDS             complement(9041..9319)
                     /locus_tag="LP452_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013882.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            9473..10168
                     /locus_tag="LP452_001145"
     CDS             9473..10168
                     /locus_tag="LP452_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2013883.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(10610..11407)
                     /locus_tag="LP452_001146"
     CDS             complement(10610..11407)
                     /locus_tag="LP452_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL2013884.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(11404..12588)
                     /locus_tag="LP452_001147"
     CDS             complement(11404..12588)
                     /locus_tag="LP452_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL2013885.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(12613..13860)
                     /locus_tag="LP452_001148"
     CDS             complement(12613..13860)
                     /locus_tag="LP452_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL2013886.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            14075..15820
                     /gene="dnaX"
                     /locus_tag="LP452_001149"
     CDS             14075..15820
                     /gene="dnaX"
                     /locus_tag="LP452_001149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL2013887.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            15840..16154
                     /locus_tag="LP452_001150"
     CDS             15840..16154
                     /locus_tag="LP452_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL2013888.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            16179..16775
                     /gene="recR"
                     /locus_tag="LP452_001151"
     CDS             16179..16775
                     /gene="recR"
                     /locus_tag="LP452_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL2013889.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            16839..17483
                     /locus_tag="LP452_001152"
     CDS             16839..17483
                     /locus_tag="LP452_001152"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL2013890.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            17498..17827
                     /locus_tag="LP452_001153"
     CDS             17498..17827
                     /locus_tag="LP452_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL2013891.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            17841..18779
                     /gene="holB"
                     /locus_tag="LP452_001154"
     CDS             17841..18779
                     /gene="holB"
                     /locus_tag="LP452_001154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL2013892.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            18815..19639
                     /locus_tag="LP452_001155"
     CDS             18815..19639
                     /locus_tag="LP452_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL2013893.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            19632..19979
                     /locus_tag="LP452_001156"
     CDS             19632..19979
                     /locus_tag="LP452_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL2013894.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            20048..20920
                     /gene="rsmI"
                     /locus_tag="LP452_001157"
     CDS             20048..20920
                     /gene="rsmI"
                     /locus_tag="LP452_001157"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL2013895.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            21029..22150
                     /gene="dinB"
                     /locus_tag="LP452_001158"
     CDS             21029..22150
                     /gene="dinB"
                     /locus_tag="LP452_001158"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL2013896.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(22204..22806)
                     /gene="nrdG"
                     /locus_tag="LP452_001159"
     CDS             complement(22204..22806)
                     /gene="nrdG"
                     /locus_tag="LP452_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL2013897.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(23121..25310)
                     /gene="nrdD"
                     /locus_tag="LP452_001160"
     CDS             complement(23121..25310)
                     /gene="nrdD"
                     /locus_tag="LP452_001160"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL2013898.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            25609..26403
                     /locus_tag="LP452_001161"
     CDS             25609..26403
                     /locus_tag="LP452_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013899.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            26393..28435
                     /locus_tag="LP452_001162"
     CDS             26393..28435
                     /locus_tag="LP452_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013900.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            28716..28865
                     /locus_tag="LP452_001163"
     CDS             28716..28865
                     /locus_tag="LP452_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL2013901.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            28876..30390
                     /gene="dltA"
                     /locus_tag="LP452_001164"
     CDS             28876..30390
                     /gene="dltA"
                     /locus_tag="LP452_001164"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL2013902.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            30387..31592
                     /gene="dltB"
                     /locus_tag="LP452_001165"
     CDS             30387..31592
                     /gene="dltB"
                     /locus_tag="LP452_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL2013903.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKIFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            31637..31870
                     /gene="dltC"
                     /locus_tag="LP452_001166"
     CDS             31637..31870
                     /gene="dltC"
                     /locus_tag="LP452_001166"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL2013904.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            31873..33141
                     /gene="dltD"
                     /locus_tag="LP452_001167"
     CDS             31873..33141
                     /gene="dltD"
                     /locus_tag="LP452_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL2013905.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            33282..34364
                     /locus_tag="LP452_001168"
     CDS             33282..34364
                     /locus_tag="LP452_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL2013906.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            34364..35167
                     /locus_tag="LP452_001169"
     CDS             34364..35167
                     /locus_tag="LP452_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL2013907.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            35237..35977
                     /locus_tag="LP452_001170"
     CDS             35237..35977
                     /locus_tag="LP452_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2013908.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            35988..36992
                     /locus_tag="LP452_001171"
     CDS             35988..36992
                     /locus_tag="LP452_001171"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL2013909.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        11873 a         6530 c         7721 g        10913 t
ORIGIN      
        1 ataaaataca agcatatcca tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa
       61 aatcgtaatc aaaggaaacc gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa
      121 tagtgcagtc gcattgattc cagctgcaat cttagctgat tccccagtga tcttggaagg
      181 tgtgccggac atacaagatg ttcattcatt aattgaaatt ttagagatca tgggcgcaac
      241 tgtccatttt tccaacaaca tactagaaat cgatcctcga gaagtcgtat cgattccgat
      301 gccgcacgga aaaatcaata gtttgcgtgc ttcttattat tttatgggaa cactgcttgg
      361 taaatttggc gaagcggtag taggcctgcc aggtggctgt tatttaggcc ctagaccgat
      421 tgacctacac gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc
      481 catgtattta cggacagaaa ataaaggatt gcgtggcaat cggattttca tggacgtggt
      541 ttcagtcgga gcaacgatca atgtgatgtt agctgctgta aaagcaaaag gacagacagt
      601 aattgaaaac gctgcacgag aaccggaaat catagatgtg gcaactttac tgaataatat
      661 gggggcaaaa gttcgaggag cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt
      721 acatggctgt cggcatttca tgattcctga ccgcatcgaa gcaggaacct atctagcatt
      781 agcagctgca gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac atttggaaag
      841 ctttatagca aaattgcagg aaattggtgt ccatatgaaa atcagcgaag atgaaatcga
      901 agtttacccc tcgaaagaat taaaacccgc aaacatcatg acctatcctt atccaggatt
      961 tgccacagat ctgcagcagc cacttactgc gttactattg atgactacag gcacttcaga
     1021 aatcatcgat acgatctatg cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg
     1081 cgctgatatt accgtggaag gaaatatgat catcgtgaat ggtccaaata aacttcacgg
     1141 cacagaagtc gtggcttctg atcttcgggc aggtgcttgt ttagtgattg ctggactatt
     1201 ggcagaaggg acaacgacga tttacaatgt agattatatc ttacgcggtt atgatcatat
     1261 tatcgagaaa ctaacagcct taggggcatc aattgaaatg atagaggaag tgggagctga
     1321 atgagcgatt atttgacaat ggcagaacta gagaacaaaa cgttaaaaga agtttatagt
     1381 tatgccaaag aatttaaaat tccttattac agtaagatga ataaaaagga attatctcta
     1441 gctgtgattc gtgcacaagc agagaaacaa ggattttatt atatggaagg aatattggat
     1501 atcgttggtc aagatggtta cggtttttta cggccaatca attatggacc aagtgcagaa
     1561 gatatctata tttctgcctc acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg
     1621 gcaggaaaag cccgtccacc taaagaatcg gaacgttatt atggtttgat gcacgtggaa
     1681 agcgttaatg gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc agccttaaca
     1741 cctctttatc cgcaaagaca gcttcaactg gaaacaacac cgcaaagact atctacccga
     1801 atgatcgata tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca
     1861 aaagcaggga aaacttctgt attaaaagaa atcgcaaatg gtatcaccga aaactatccg
     1921 gaagtagaat taatcgttct actgattgat gaacgaccgg aagaagtaac ggatctagaa
     1981 cgaagcgtga aaggcgacgt ggtatcttct acatttgatc tgcagccgca aaaccatact
     2041 cgagtttctg aattagtatt ggaacgcgca atgcgtttag tcgaagataa acgagatgtc
     2101 gttatcttga tggatagtat tactagactt gctcgggcct ataacttagt cgttccacca
     2161 agcggacgta cattgagtgg cgggattgac ccagccgcat tgtataaacc taagaaattt
     2221 ttcggggcag caagaaacat cgaagaaggt ggcagtttga cgattttagc gaccgcttta
     2281 gtcgatacag gaagccgtat ggatgacgtg atctatgaag aattcaaagg aactggaaat
     2341 ctagaattac aactttcacg tgaattatct gaacgtcgga tcttcccagc catcgatatc
     2401 aaaaaatcag gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt agaagaaatt
     2461 tggaaacttc gaaaaaatat gactggagat tcattagaat atacggaaca actgatccgc
     2521 tttttgcgta aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga tgtctccttt
     2581 ggtaaaaaaa atccgaccag aaatctgaaa agataattgc aaccttgact gaaacatgtt
     2641 aagatgttaa tgttgtaaat cgacaaatca actctggttc agcaaatgga gcagggcaaa
     2701 ggagcgaaaa aatcatgaaa gaaaacatcc atccagatta ccacccagta gtattcatgg
     2761 attcaacaac tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg
     2821 aatgggaaga cggtaataca tacccagtca tccgtgtcga agtaacttct gactcacatc
     2881 cattctacac tggacgtcaa aaattcacac aagcagacgg acgtgtggac cgtttcaaca
     2941 aaaaatacgg tctcaaagac gcaaacgctg cggaataata aaaacgctga taactcaacg
     3001 tttagcagac tatctgatag ggatagtctg tttttttatg ttttttgcca atttaaaact
     3061 tcgtttgatg ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa aactagtata
     3121 tagttttaat tatccacttg ataaaaacac cttatattaa cgaaatgaag aggggttatt
     3181 tcgaagtaaa aaaccattta cctaaaatta gaagctaagc tttcctttca aggaaggctt
     3241 tttgttttgt tatagacttg cgattgtttg tgagctagat cgttcatttt aatgacttat
     3301 acacgcggat gatgggtctt tttcctctgt tcattcataa tattgaacgg tacagctagc
     3361 gatgctatac tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt
     3421 ttatgaggtt tgatcaattg atcgcagaag aactgattgt tttcgatcca tcgatagatt
     3481 caaaaggatc attatttgag cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa
     3541 atgttcaaaa agtcgttaag gcactattca aacgagagga ggagatctcc acaggaatag
     3601 aagatggatt tgggattccc catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt
     3661 tttttcatac agccaaatta acggactata gcgggataga tgaaatgcca attgaatata
     3721 tttttgccat cgtagttcct gaaaaatcag cagatatgca tctagagatt ctcagcacac
     3781 tctccagacg attgatgaat gcagatttta gaaagggaat aaaagaagcc actacgccag
     3841 cagatatcat cagacagatt agtcaagaat gaaaatagta gagtctttgt ccgagtatcg
     3901 atagtgaaga aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac
     3961 tggaattgct catacttata tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa
     4021 ccatcaagca aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga
     4081 aatcaaagag gcagatgcaa tcattcttgc agtagataaa gaaatcgaat tggaccgatt
     4141 tgccgggaaa aaagtaaagc gtgtctctac aagccgagca atcaaagaac cgcaagtagt
     4201 aatagacgaa gcattgagag atattggcgt attcgttgtg tctaatgaaa aagaaccagc
     4261 tgcaaatgag aaaaagccga caatctataa tcatttcatg aatggtgtga attacatgct
     4321 tccatttgtg atcgcaggcg gaatcatcat cgcactgagt tttgcctttg ggattacagc
     4381 ggctgatccc aactcagcag actataatgt gttagcagct gcattttcaa ggatcggagg
     4441 agatacagcg tttgccatga tggttcctgc gttaggcgcg gggattgcta catcgattgc
     4501 tggaaaagca ggttttgccc caggaattgt tgccggatta ctggcctcta caggtggttc
     4561 aggttttctt ggcggaatga ttggtggcgt gctagcaggg tatatctgtg attttctggc
     4621 aaataaaatt agcgtaaaaa aagaattttc tgctatttat cagctgattg tcgtcccctt
     4681 tttatcgatc ttaattattg ggcttttgat ggtatttgtg attgaacaac cgatcacatg
     4741 ggtgttggat gctttgacaa attggctgaa tagcttggga aatacatccg ggctattgtt
     4801 cggattactt atcgggatca tgatggcagc tgatatgggt gggccgatca ataaatcgac
     4861 ttcgactttc tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat
     4921 ggctgcagga atgacacctc ccctaggtat tgctttagcc actttattat tcaaacataa
     4981 atttaccaaa gaagaacgga cttcaggtca atcatgctgg attttaggcg cttcgtatat
     5041 tacagaaggt gctataccgt ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat
     5101 gacaggttca gcagtcgctg cagggatttc catggcagta ggagttactt caatggcacc
     5161 tcatggaggg atttgggtga tgctaattcc aaatgttatg aacaatttac ctatgtatgt
     5221 cttggcaatt gcatcaggta cagtcactac agcctttatg cttggactat tgaaaaaaac
     5281 agtgaaagag aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa atgaggtgga
     5341 agcgtaatgt tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca
     5401 gtaccagcct tcaatatttg cagctatgat atgatgaaag caatcatcga agaagcagag
     5461 agattagaag caccagtaat cttagaaatt catcctgatg aaatcgccta tttaagtgat
     5521 gagtttgttg cggcagtaag agcctatgct tatacaagta aactgccgat cgttatccac
     5581 ttggatcatg gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc
     5641 gtgatgatcg atgcgtcttt gaaatcattc gacgacaatg tacagattac gaaaaaagtg
     5701 gttgcgttag ctcatgcggt aaatgtctca gtggaggctg agcttggaac gattgggaat
     5761 aatggttctg ctgaaggagg tgctgaaaca atcatttata cagatcctga tcaagcgaag
     5821 atttttgtga aagaaacagg aatcgataca cttgctgtag caattggaac agcgcatggc
     5881 ttgtatccga aaaacagaac tccaaaatta aatatcgact tactgaaaga attgaaccaa
     5941 caaatcgatc ttccatttgt tttacatggc ggttcaggta atccggacgc agaagtcagc
     6001 gaagcagtaa aatacggagt tgccaaagtg aatttgagct cagatcttaa aagcgtcttt
     6061 ttcaatgctt tgagagaaat attgagtgaa aatccatcga tgtacgagcc gaatgcaatt
     6121 tatccagcag ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga aattttgaac
     6181 acagtaggca aggctagttt atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa
     6241 tagtcttgtc ataccctctt aaccacacac gatgttcaga agtagttttt tcgaaaaatt
     6301 tcttcttatt tctcgaagcc aatcacttct gtcacaacct cttttttcct gttactatat
     6361 aaagtaaatg gggtgatttt atgttgaatg aagtggagaa tagacagcag gagattgtga
     6421 attatctaaa aaacaaccaa tttgcaagag tcaccgattt gatcgagtta gtaaattata
     6481 gcgaagccac gattaaacgt gaccttgttt ttctggaaaa aagtggattg atccgtagag
     6541 ttcgcggtgg cgccatgctt attgacaatc gaaaaattga cgtgccttac ctgatgaaga
     6601 tcaccaaatt agaagaagaa acagaaaaac gtaagattgc agatacagct tcttctttga
     6661 ttcgagatga tatggtctta ttcttggatt ccagtacaac ctctttacac ttagtcagaa
     6721 acctagcaag atttgatggt ttgcagatca ttacgaatgg catcgtcaca gctgctatgt
     6781 tgagtgaata tacaacggca aaagtatctg tattgggagg ggcaattgtt ccgaaacgag
     6841 caacaatcaa cggttcaaaa gcctataacg atgcattgtc atattatgcg gatttagcga
     6901 taatgagttg tcgcgggcta gatttcagcc aaggagtaac agaaactcat gaaggagagg
     6961 caatggtcaa aaaagctttt agaaaacaat cagaccggct tgccgtttta gttacacagc
     7021 aaaaaattgc acataaattc atgcatcaag ccattgcttt tcatgaaatc gattatctgg
     7081 tgacaagtaa agaactgaca aaagaacagt atgcgaaagt aaaggaacac catatcgaat
     7141 gtctgtacgg ataaattatt acgtgaatgt tctaagtggc ttcctcattt ttagatagga
     7201 ccttatcttt gctcgcttct ctcagccgat ggtataataa tgaaaaggtt atcttgttag
     7261 ataatcttac ttttttatcc aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt
     7321 ggtagtgaag gcaatcatga tctgggcgat tctgtgggtc gtgctgacag gtttatacgg
     7381 tgttagcttt atggattcaa cgattgttgg ggtaattatt gtagtcatga tctatgtgct
     7441 aggggatctg ttgatcttac gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg
     7501 ttcagctgca gttatcttgt ggctctatct atattttatg aatgatacag tcgatatttg
     7561 gatgaaagta ttgattcctg ctctattgat cggtgtggca gaatggttct tccacaagtg
     7621 gttgctaagt caaggcatcg taccagatga acggaaaatg gaataaatca acagttaaac
     7681 tattcttatc gataaatgaa agctttaaaa ataaatagat gtttaattaa gaataatcat
     7741 aaactatttt tactcaaaaa accgctcaga ttattttcag atctgaacgg ttttttttat
     7801 ctattcatca agatcaaatt tcatttttca atgattcaat caattcgccc tctgcttcat
     7861 gactatgttt tcggccatta acaagataga gtgctgtaca tatcaatgga atgactccgg
     7921 caatcacata caatcctcta aatgaagtaa gagattttag catccccatc aaataaggac
     7981 caacgcctaa gcctaggtct aaaccaataa agtaggtaga taaagcaata ctgattcgat
     8041 gttcgcggac gatttttaaa caaacagcct gtccatttga cataaacgta ccataaccta
     8101 ggccgactaa tgctccagag ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa
     8161 ataatcctgc tgttaagaat aaatagctag gatacatcac gtattgttct cctttgacat
     8221 cgaaaatcct tccagtcaat ggacgtgtaa atgtgatgac caaagcatat acgacgaaga
     8281 aaaatgaccc agcagcaact aagtggatta cttttgtata agaagataaa aatgataaaa
     8341 cacttgaata agaaagaccc atcaaaaagg caattcctgt aatgaaaagt gctttctttt
     8401 caataaaact attgatattc catgagcgaa gagatatttt ttgttcttca gataatggaa
     8461 tattttttac aggaaagaca aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa
     8521 accagataat gaaacgaaaa tcagtcatat tcagcaaaat cattcctaaa aatggcccaa
     8581 cagcggcagc taaactagta cttaatccat aataattgat gccttcccct tttttagatg
     8641 cagggatata tgcagtaacg atagcatttg tagctgtaga aactgtaccg tagccgaatc
     8701 cattcaagaa tcgaactaaa tacaatgccc cgatagttgg cgtataaaga tatgctaatg
     8761 ttgaaaggag atagaagatc gcgccgcctc ttaaggtaaa cttccgacca aatagttcta
     8821 actgtttccc catgaataag cgtgccagca atgttccgat gatatagatc ccagaagcaa
     8881 aacctgcttg gctcaagctg gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca
     8941 tcaacagata ataaatcagg tagacaataa aattgatcaa tgtgattcca ataaaatatt
     9001 tattaaataa tttcttttcc atgtatgtta tacattcccc ttaagctgaa tatgctttta
     9061 atttttgtat ttgaacttct acatcgtgat aattggatgg atagcgatca acaagttggc
     9121 gaataaataa atctcttgta gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg
     9181 gattctcatc cactgctttg atcaattcga tcgagttttg atacgcatca tttaaaaaga
     9241 ctaaagaagc tttcatatca aatcttgttt gatatgctgt ttctaatcga ttaatgattg
     9301 tgttcatcca gtaattcatg ttttttccct ctattcttaa aaaattttac ttctgcagta
     9361 taacgaataa aaaaaaggaa aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg
     9421 ttaatatcat tagtaaaata cctttggact ttaaaaagtt ttaaagtact gtatgatctt
     9481 tttagatgaa agtttggatg ggggagcatc attggatagt agagtcttaa aggaatattt
     9541 ggataaaaat gattttccga ttatacgcaa aggatatcat aaatatttga cattcgaagg
     9601 gatggaagat tcccatacgt atatcttgaa agagggaacg gtaaaagcaa gtgttatttc
     9661 aaatgatggg agagaattta accttcgcta tatcaaagat cttgaaatcg tctctctttt
     9721 aaaagacgaa tattcacaat tcatcgattc gccgtataat attcgagttg aatcagaata
     9781 tgcagagttt tatcgaatca atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa
     9841 gctacaacac tatgtaaaag agtattaccg ctatcgtctg ctttattcga tgaaaaaaat
     9901 gcagcaaatg ctattaaacg gaaagctggg agctgtttgt acgcaacttt acgaattata
     9961 tgatttattc ggtgtgcgga cagaagaagg gatgctgatc gatttttcga tcacgaatga
    10021 agaaatcgcc aaattttgtg gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca
    10081 actgaaaaaa gatggtatta tcaccgttaa agaccatcat atcgttattt tgaatatgga
    10141 cttgttagaa gacaatatta tcttttgatg aacaaagagg ttgtgaaatc agcgttttga
    10201 cctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt tttttgagct
    10261 aataccgagg gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa
    10321 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
    10381 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
    10441 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
    10501 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
    10561 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
    10621 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
    10681 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
    10741 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
    10801 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
    10861 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
    10921 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
    10981 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
    11041 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
    11101 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
    11161 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
    11221 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
    11281 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
    11341 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
    11401 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
    11461 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
    11521 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
    11581 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
    11641 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
    11701 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
    11761 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
    11821 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
    11881 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
    11941 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
    12001 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
    12061 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
    12121 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
    12181 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
    12241 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
    12301 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
    12361 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
    12421 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
    12481 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
    12541 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
    12601 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
    12661 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
    12721 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
    12781 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
    12841 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
    12901 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
    12961 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
    13021 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
    13081 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
    13141 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
    13201 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
    13261 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
    13321 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
    13381 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
    13441 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
    13501 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
    13561 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
    13621 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
    13681 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
    13741 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
    13801 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
    13861 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
    13921 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
    13981 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
    14041 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
    14101 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
    14161 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
    14221 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
    14281 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
    14341 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
    14401 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
    14461 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
    14521 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
    14581 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
    14641 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
    14701 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
    14761 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
    14821 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
    14881 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
    14941 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
    15001 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
    15061 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
    15121 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
    15181 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
    15241 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
    15301 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
    15361 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
    15421 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
    15481 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
    15541 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
    15601 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
    15661 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
    15721 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
    15781 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
    15841 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
    15901 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
    15961 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
    16021 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
    16081 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
    16141 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
    16201 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
    16261 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
    16321 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
    16381 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
    16441 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
    16501 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
    16561 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
    16621 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
    16681 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
    16741 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
    16801 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
    16861 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
    16921 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
    16981 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
    17041 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
    17101 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
    17161 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
    17221 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
    17281 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
    17341 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
    17401 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
    17461 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
    17521 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
    17581 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
    17641 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
    17701 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
    17761 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
    17821 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
    17881 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
    17941 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
    18001 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
    18061 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
    18121 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
    18181 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
    18241 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
    18301 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
    18361 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
    18421 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
    18481 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
    18541 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
    18601 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
    18661 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
    18721 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
    18781 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
    18841 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
    18901 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
    18961 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
    19021 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
    19081 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
    19141 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
    19201 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
    19261 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
    19321 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
    19381 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
    19441 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
    19501 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
    19561 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
    19621 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
    19681 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
    19741 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
    19801 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
    19861 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
    19921 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
    19981 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
    20041 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
    20101 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
    20161 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
    20221 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
    20281 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    20341 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    20401 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    20461 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    20521 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    20581 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    20641 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    20701 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    20761 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    20821 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    20881 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    20941 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    21001 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    21061 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    21121 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    21181 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    21241 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    21301 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    21361 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    21421 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    21481 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    21541 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    21601 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    21661 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    21721 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    21781 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    21841 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    21901 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    21961 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    22021 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    22081 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    22141 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    22201 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    22261 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    22321 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    22381 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    22441 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    22501 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    22561 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    22621 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    22681 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    22741 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    22801 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    22861 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    22921 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    22981 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    23041 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    23101 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    23161 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    23221 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    23281 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    23341 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    23401 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    23461 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    23521 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    23581 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    23641 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    23701 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    23761 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    23821 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    23881 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    23941 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    24001 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    24061 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    24121 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    24181 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    24241 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    24301 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    24361 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    24421 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    24481 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    24541 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    24601 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    24661 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    24721 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    24781 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    24841 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    24901 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    24961 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    25021 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    25081 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    25141 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    25201 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    25261 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    25321 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    25381 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    25441 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    25501 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    25561 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    25621 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    25681 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    25741 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    25801 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    25861 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    25921 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    25981 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    26041 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    26101 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    26161 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    26221 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    26281 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    26341 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    26401 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    26461 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    26521 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    26581 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    26641 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    26701 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    26761 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    26821 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    26881 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    26941 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    27001 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    27061 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    27121 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    27181 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    27241 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    27301 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    27361 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    27421 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    27481 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    27541 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    27601 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    27661 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    27721 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    27781 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    27841 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    27901 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    27961 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    28021 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    28081 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    28141 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    28201 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    28261 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    28321 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    28381 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    28441 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    28501 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    28561 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    28621 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    28681 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    28741 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    28801 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    28861 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    28921 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    28981 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    29041 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    29101 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    29161 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    29221 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    29281 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    29341 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    29401 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    29461 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    29521 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    29581 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    29641 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    29701 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    29761 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    29821 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    29881 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    29941 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    30001 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    30061 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    30121 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    30181 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    30241 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    30301 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    30361 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    30421 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    30481 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    30541 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    30601 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    30661 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    30721 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    30781 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    30841 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    30901 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    30961 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    31021 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    31081 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    31141 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    31201 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    31261 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa atttttaaaa
    31321 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    31381 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    31441 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    31501 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    31561 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    31621 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    31681 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    31741 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    31801 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    31861 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    31921 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    31981 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    32041 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    32101 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    32161 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    32221 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    32281 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    32341 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    32401 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    32461 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    32521 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    32581 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    32641 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    32701 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    32761 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    32821 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    32881 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    32941 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    33001 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    33061 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    33121 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    33181 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    33241 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    33301 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    33361 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    33421 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    33481 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    33541 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    33601 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    33661 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    33721 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    33781 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    33841 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    33901 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    33961 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    34021 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    34081 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    34141 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    34201 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    34261 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    34321 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    34381 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    34441 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    34501 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    34561 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    34621 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    34681 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    34741 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    34801 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    34861 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    34921 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    34981 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    35041 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    35101 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    35161 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    35221 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    35281 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    35341 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    35401 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    35461 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    35521 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    35581 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    35641 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    35701 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    35761 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    35821 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    35881 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    35941 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    36001 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    36061 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    36121 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    36181 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    36241 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    36301 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    36361 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    36421 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    36481 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    36541 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    36601 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    36661 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    36721 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    36781 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    36841 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    36901 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    36961 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    37021 tggattccgc tattttt
//
LOCUS       DAFELO010000021        36821 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFELO010000021
VERSION     DAFELO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.021"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            318..2096
                     /gene="uvrC"
                     /locus_tag="LP452_001172"
     CDS             318..2096
                     /gene="uvrC"
                     /locus_tag="LP452_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL2013910.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            2241..3140
                     /locus_tag="LP452_001173"
     CDS             2241..3140
                     /locus_tag="LP452_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013911.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            3133..4395
                     /locus_tag="LP452_001174"
     CDS             3133..4395
                     /locus_tag="LP452_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013912.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            4558..6456
                     /locus_tag="LP452_001175"
     CDS             4558..6456
                     /locus_tag="LP452_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL2013913.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(6755..7474)
                     /locus_tag="LP452_001176"
     CDS             complement(6755..7474)
                     /locus_tag="LP452_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL2013914.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            7811..8143
                     /locus_tag="LP452_001177"
     CDS             7811..8143
                     /locus_tag="LP452_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013915.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            8130..10133
                     /locus_tag="LP452_001178"
     CDS             8130..10133
                     /locus_tag="LP452_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL2013916.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            10152..10622
                     /locus_tag="LP452_001179"
     CDS             10152..10622
                     /locus_tag="LP452_001179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL2013917.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            10643..11227
                     /locus_tag="LP452_001180"
     CDS             10643..11227
                     /locus_tag="LP452_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL2013918.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            11242..12228
                     /locus_tag="LP452_001181"
     CDS             11242..12228
                     /locus_tag="LP452_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL2013919.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            12218..12529
                     /locus_tag="LP452_001182"
     CDS             12218..12529
                     /locus_tag="LP452_001182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL2013920.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            12551..14332
                     /locus_tag="LP452_001183"
     CDS             12551..14332
                     /locus_tag="LP452_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL2013921.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            14325..15701
                     /locus_tag="LP452_001184"
     CDS             14325..15701
                     /locus_tag="LP452_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL2013922.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            15708..16343
                     /locus_tag="LP452_001185"
     CDS             15708..16343
                     /locus_tag="LP452_001185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL2013923.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            16373..17728
                     /locus_tag="LP452_001186"
     CDS             16373..17728
                     /locus_tag="LP452_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL2013924.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(17920..19314)
                     /locus_tag="LP452_001187"
     CDS             complement(17920..19314)
                     /locus_tag="LP452_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL2013925.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIYNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            19406..20377
                     /locus_tag="LP452_001188"
     CDS             19406..20377
                     /locus_tag="LP452_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL2013926.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            20374..21156
                     /locus_tag="LP452_001189"
     CDS             20374..21156
                     /locus_tag="LP452_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL2013927.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(21325..21696)
                     /locus_tag="LP452_001190"
     CDS             complement(21325..21696)
                     /locus_tag="LP452_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2013928.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            21830..22273
                     /locus_tag="LP452_001191"
     CDS             21830..22273
                     /locus_tag="LP452_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL2013929.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(22316..23125)
                     /gene="yidA"
                     /locus_tag="LP452_001192"
     CDS             complement(22316..23125)
                     /gene="yidA"
                     /locus_tag="LP452_001192"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL2013930.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(23144..24511)
                     /locus_tag="LP452_001193"
     CDS             complement(23144..24511)
                     /locus_tag="LP452_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2013931.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(24495..25325)
                     /locus_tag="LP452_001194"
     CDS             complement(24495..25325)
                     /locus_tag="LP452_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL2013932.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            25488..25925
                     /locus_tag="LP452_001195"
     CDS             25488..25925
                     /locus_tag="LP452_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL2013933.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            26001..26621
                     /locus_tag="LP452_001196"
     CDS             26001..26621
                     /locus_tag="LP452_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL2013934.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            26810..27316
                     /locus_tag="LP452_001197"
     CDS             26810..27316
                     /locus_tag="LP452_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL2013935.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            27333..28232
                     /locus_tag="LP452_001198"
     CDS             27333..28232
                     /locus_tag="LP452_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL2013936.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            28319..29203
                     /locus_tag="LP452_001199"
     CDS             28319..29203
                     /locus_tag="LP452_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2013937.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(29395..29466)
                     /locus_tag="LP452_001200"
     tRNA            complement(29395..29466)
                     /locus_tag="LP452_001200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29431..29433),aa:Arg,seq:ccg)
     gene            29526..30860
                     /gene="rpoN"
                     /locus_tag="LP452_001201"
     CDS             29526..30860
                     /gene="rpoN"
                     /locus_tag="LP452_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL2013938.1"
                     /translation="MKFEQNFSQKQQQTQKLTMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            31009..32100
                     /locus_tag="LP452_001202"
     CDS             31009..32100
                     /locus_tag="LP452_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL2013939.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            32141..33142
                     /gene="gap"
                     /locus_tag="LP452_001203"
     CDS             32141..33142
                     /gene="gap"
                     /locus_tag="LP452_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL2013940.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            33252..34442
                     /locus_tag="LP452_001204"
     CDS             33252..34442
                     /locus_tag="LP452_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL2013941.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            34546..35301
                     /locus_tag="LP452_001205"
     CDS             34546..35301
                     /locus_tag="LP452_001205"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL2013942.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            35417..36715
                     /gene="eno"
                     /locus_tag="LP452_001206"
     CDS             35417..36715
                     /gene="eno"
                     /locus_tag="LP452_001206"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL2013943.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        12199 a         6450 c         7832 g        10340 t
ORIGIN      
        1 attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa
       61 agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt
      121 ttccaccaac acaaaatatt atagaaccaa aataagctga gggtgggact gaatccagtt
      181 ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt tttgagtata
      241 attacgttgt caggctggat tgtttttttg acagacatgc tataataact ttaattgaat
      301 atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta cttcctgatc
      361 agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc ggcaaagcca
      421 aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg aaaacggaac
      481 ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat atcgaagcat
      541 tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt atgttaaagg
      601 atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt ttggtgatta
      661 ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat gtcggtgcgg
      721 cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc aaacctagtc
      781 agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct tattatttcg
      841 acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt ttaaacggag
      901 gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct gaaaacatgg
      961 agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca atcatgactc
     1021 gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat gcgattgaca
     1081 aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt gaacgagatg
     1141 tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac ctcggtcagt
     1201 tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat acgatcgaca
     1261 agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga ggagaaaaga
     1321 aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa cggtttgatc
     1381 tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga aacgcgatga
     1441 atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg gggacgaatc
     1501 ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat
     1561 ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa gtcatctatc
     1621 gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg
     1681 gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta ggaatcgata
     1741 ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg ctattcggta
     1801 tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta ctccagcgaa
     1861 tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca
     1921 gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc
     1981 ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa ttacaagaaa
     2041 tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag gaatgaggaa
     2101 agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt
     2161 tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata tatgtgattt
     2221 cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact tttgggaact
     2281 acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg gggctaattg
     2341 gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt ttgacacaag
     2401 accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat gatgtcatcg
     2461 gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac caactgcttt
     2521 attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc gatttttgga
     2581 tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg tctaaaggaa
     2641 atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg atcattttgg
     2701 atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat ggaatattgg
     2761 aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat aatgtagaaa
     2821 aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac
     2881 atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt ttaagccaac
     2941 aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag
     3001 agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg actcagttcg
     3061 gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc aaattgaaag
     3121 caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt aaaaaatgtt
     3181 aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg tatcatttat
     3241 gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt attggctgat
     3301 aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga gttcaaagca
     3361 gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga tgcgtattta
     3421 gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa ttcattagga
     3481 caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat gcttcgagca
     3541 agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc aagtttgact
     3601 cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga taactcgaca
     3661 gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat tttgacgtat
     3721 gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc
     3781 ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt tttacttgtt
     3841 gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag tcaattcaaa
     3901 gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt gattggacca
     3961 ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc tgttttagca
     4021 gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat tttgccagtg
     4081 acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc agatccaaac
     4141 aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat tatgccggtt
     4201 cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt ggtacttgta
     4261 gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtg
     4321 tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat gagaaatgaa
     4381 cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta ttgttcccag
     4441 cttctttttt gtatagacct atttctattt ttttattata ttggatatat atattcattg
     4501 aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg cgttttaatg
     4561 gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt catcacggaa
     4621 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagcgat
     4681 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
     4741 gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag ccaattcact
     4801 atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa acaagataaa
     4861 tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa atacactcga
     4921 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
     4981 atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc
     5041 ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca aaaatctgtc
     5101 atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc agccaaagta
     5161 atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca tgatattttg
     5221 tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg gattgccgca
     5281 cgctacaact atttatttga attagagaaa gaatatggat taaatttacg tccattattt
     5341 tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa caggaaagat
     5401 ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc tatcctccaa
     5461 ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa
     5521 ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca gctctatgag
     5581 ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt cttggaagag
     5641 gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag aatgcaaaaa
     5701 cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtactg
     5761 ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga attagaccaa
     5821 tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga aagcgcaaaa
     5881 gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg gagcgggcca
     5941 ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt tctcacagag
     6001 cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga agagtggttc
     6061 agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat catcaatgga
     6121 cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg aaaagcattt
     6181 gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc tggctactca
     6241 ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt gcctattgaa
     6301 caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc
     6361 ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca gattactcaa
     6421 ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc aacattgctc
     6481 aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg aattagctca
     6541 aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact caggtcaggg
     6601 cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
     6661 cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt
     6721 gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca aagaaatcga
     6781 ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat tggacaccaa
     6841 ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg ttggaaaagg
     6901 cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag gaattcacaa
     6961 agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg acttcgtgag
     7021 ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt ggatataatg
     7081 aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt cctcggcaga
     7141 ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt tcgaattggt
     7201 ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa agttgcggac
     7261 taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg
     7321 aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa actgcatcca
     7381 caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc gctcgaatca
     7441 gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa gtagactaat
     7501 tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac ttatctgttc
     7561 ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa
     7621 tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc
     7681 aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta
     7741 agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa
     7801 tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga
     7861 aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag aagaaaaaga
     7921 gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt tggtagaaga
     7981 aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga aagcagaagc
     8041 agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc aagcaatcgg
     8101 tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc
     8161 tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaatg
     8221 ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa aaatattttc
     8281 cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat cggctgaaag
     8341 acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc
     8401 atttgaaaca tcaagaactt tccttacaag acttggaagc aaattattcc gaagaaaatt
     8461 tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa
     8521 aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg
     8581 ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa
     8641 attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg gaagaaatag
     8701 attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgcta
     8761 tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc
     8821 ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag cagcaaaaaa
     8881 agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa tgggtagaag
     8941 aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc catgccactt
     9001 atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg atgcatgtgt
     9061 taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca aactcagaag
     9121 agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa ccttttgaat
     9181 tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg ccttggatga
     9241 caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac ggattgctga
     9301 tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg atgaaacgtt
     9361 tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg atctacagtt
     9421 catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc
     9481 tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac
     9541 agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa gattacttga
     9601 gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt attgcattga
     9661 ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg ctatcgatca
     9721 ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg
     9781 ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat ttagtcagtt
     9841 atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca ttcaatatgc
     9901 tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg atcatcgctt
     9961 tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt gcacgattgc
    10021 agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt gacccgctga
    10081 aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa taattggagg
    10141 atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcagtat
    10201 tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt gttggaatga
    10261 caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga caggctttga
    10321 ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc tttttgattt
    10381 ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta ggcgcttctc
    10441 tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt gcagcagcag
    10501 gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga
    10561 tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta ttgaatgcct
    10621 aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaatga
    10681 agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa
    10741 ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat
    10801 cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag aagtacgaca
    10861 agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg caatagctga
    10921 aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat tgtattcatt
    10981 acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt atctttctca
    11041 gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac
    11101 tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt
    11161 cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt
    11221 cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga
    11281 gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa
    11341 gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa
    11401 tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa
    11461 tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt ccataatctg
    11521 aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta tatcgacgat
    11581 ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactc
    11641 cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt
    11701 cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc
    11761 gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat
    11821 atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta
    11881 ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg
    11941 gaagattaca cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc
    12001 cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta tctaagttca
    12061 ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac
    12121 gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc
    12181 gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt
    12241 tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac
    12301 aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat
    12361 ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaaggaaca
    12421 aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga
    12481 agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga
    12541 gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga
    12601 gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg
    12661 agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg
    12721 aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc
    12781 agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat
    12841 cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa
    12901 atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg
    12961 gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg
    13021 aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga
    13081 aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg
    13141 gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga
    13201 tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg
    13261 gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt
    13321 gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat
    13381 tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa
    13441 catgccagtg gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt
    13501 ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc
    13561 acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta
    13621 tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc
    13681 agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg gaggggatat
    13741 ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc
    13801 cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta
    13861 ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt
    13921 caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt
    13981 agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg
    14041 tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa
    14101 acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc
    14161 gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg
    14221 acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat
    14281 caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc
    14341 gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat
    14401 atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg
    14461 aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac
    14521 gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga
    14581 tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg
    14641 aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg
    14701 agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa
    14761 aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc
    14821 gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa
    14881 tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc
    14941 gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc gctactcctc
    15001 gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta
    15061 tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag
    15121 aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct actttattcg
    15181 aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa
    15241 tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac
    15301 agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc
    15361 cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg
    15421 cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattag
    15481 ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg
    15541 agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa
    15601 cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca
    15661 aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga
    15721 atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa
    15781 gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga
    15841 ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg
    15901 attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta tttgttttgc
    15961 cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg
    16021 tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga
    16081 attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt
    16141 tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac
    16201 gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt
    16261 atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa
    16321 aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa cgatgattaa
    16381 acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat taggattttt
    16441 tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca
    16501 tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg taacgggatt
    16561 gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt
    16621 gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa
    16681 gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt
    16741 atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta tccaattgat
    16801 tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga aaggtatctg
    16861 gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga
    16921 cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat
    16981 ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa
    17041 gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt
    17101 cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga atatgtttca
    17161 acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt
    17221 tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg
    17281 cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat
    17341 ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc gtaccatccg
    17401 acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt
    17461 cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat
    17521 tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga
    17581 cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt
    17641 attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata
    17701 tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag
    17761 taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc
    17821 gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt ttttattgaa
    17881 aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat atttttttat
    17941 tttttctttt acgatactta gtccagaagc taaaatttca aggttattag aggataggga
    18001 aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg
    18061 gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag gatcaactgg
    18121 aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa
    18181 aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat ttgcttcatt
    18241 tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca catttatatt
    18301 ataaattgct tgttctatct tgctttggac atgtactgga actacaagat aagcaactcg
    18361 caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa
    18421 attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc
    18481 atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt
    18541 gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat
    18601 cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc
    18661 aattggaacg atctctaaat gatataactg agccaattca atgaaattag cgtatgtata
    18721 tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc
    18781 attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt
    18841 taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag
    18901 caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt
    18961 acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt
    19021 agagatggtg atggactcaa agacattggg agaattaaat gtgccacttc caatattcgt
    19081 atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa
    19141 atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt
    19201 ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt
    19261 ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttctc
    19321 cactccttaa aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta
    19381 atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc
    19441 ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg
    19501 taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag gcaaaaatat
    19561 gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa aagtcattac
    19621 cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt
    19681 tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg
    19741 aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact
    19801 tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg
    19861 tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc
    19921 taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc
    19981 agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa
    20041 agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag
    20101 agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctgc
    20161 cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg
    20221 tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc
    20281 gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata
    20341 tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat
    20401 gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg
    20461 ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt
    20521 acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat
    20581 ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct
    20641 gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat
    20701 tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt gtctgaatat
    20761 gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta
    20821 ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt
    20881 gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga
    20941 accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc acattctaat
    21001 cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca
    21061 tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat aggaggaaaa
    21121 gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga attaaaaata
    21181 aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga cgcaaacttt
    21241 ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt tttattcaat
    21301 caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat aaccatcagg
    21361 gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt cagtaatctc
    21421 aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg ctatagcgat
    21481 atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag tcaactctaa
    21541 ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc
    21601 acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg atgcttcaag
    21661 atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc ttttcctttt
    21721 actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc atgagaaatt
    21781 tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca tggataaacc
    21841 aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag catatattgt
    21901 catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt tttttcttcc
    21961 tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg agctgataga
    22021 agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg agtattttta
    22081 ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg ctgattcttg
    22141 gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga gccctcaaga
    22201 agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa
    22261 aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt atttattaca
    22321 gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat
    22381 ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga ccagcatatt
    22441 caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct tgatcaatac
    22501 caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta tttagtactt
    22561 cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac
    22621 gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg acgatgttca
    22681 tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact aaacttgctt
    22741 cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg tcatcgatcg
    22801 aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa tcttcatgtg
    22861 tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt gccccgttat
    22921 aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt tcctggacac
    22981 ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa
    23041 tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta ccatcgatgt
    23101 cgatagcgac tagtttgata gacataattt cctccaatat attttaattt atttcaatca
    23161 atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat aaatcataat
    23221 gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca tctccttgtt
    23281 cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc aaagttccgt
    23341 catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat
    23401 aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat ccgacctttt
    23461 ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa aaagtagcag
    23521 atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata ggatcagcag
    23581 attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg aggtattctt
    23641 ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt tcaaaactgc
    23701 tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa attacttcca
    23761 taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg ctaacgatat
    23821 aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca taaggacgaa
    23881 tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg aaataagcat
    23941 cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt tggacaactt
    24001 gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa ccttcttcta
    24061 ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct acagtgatgg
    24121 cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca tgaccgacat
    24181 catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag cctccgttgc
    24241 ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt gaaatctcat
    24301 aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg aaagaagaag
    24361 tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc aaatctaaga
    24421 ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact ttttccatag
    24481 gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt attacgttgt
    24541 tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt tagccagtcg
    24601 gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc aaatgcttct
    24661 ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc cattgtagca
    24721 ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga ttgataaaat
    24781 cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta catcataaca
    24841 gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc atggatgctt
    24901 aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat cggtgccttt
    24961 ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat ggttattttt
    25021 tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc tgatacgatc
    25081 cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag tgtttggatc
    25141 ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt gcagtcatat
    25201 tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa tatatctgtt
    25261 aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg aatcgcaggg
    25321 ttcattcaac cggctccttt gtataaattc atattttcta caatattata ctctaaaatc
    25381 aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt gacaaaacaa
    25441 aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg gaattatcac
    25501 aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag
    25561 atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg tacatacgtt
    25621 ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa ttagttccag
    25681 atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa tttggctata
    25741 aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt gctactttta
    25801 cgaaagaact gcatgttagt ttgaaagacc gtcccacagg agggaaggtt cgaatcgatt
    25861 atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg gattttacag
    25921 cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata aggagtagac
    25981 tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat
    26041 tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc atggattttt
    26101 ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa attcgtgatt
    26161 cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca ttaggggata
    26221 atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg
    26281 gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct
    26341 ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa gatacagatc
    26401 tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat gaagaaatcg
    26461 ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat gatgaagaag
    26521 acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat gatgatgaag
    26581 attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt tcttacgaga
    26641 ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat ttttgtcaca
    26701 ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa aattgaaact
    26761 tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa tgatttttac
    26821 tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg gactcatcga
    26881 aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata aagaagaggc
    26941 cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa aagaaaaaga
    27001 gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat tagatagttg
    27061 gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc cccaacagtt
    27121 acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag acagtccgga
    27181 aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa ttgcgactat
    27241 cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga atgctttacg
    27301 tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa
    27361 aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc
    27421 agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag agacgtctcc
    27481 aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt ctttttaata
    27541 gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt gaattgatct
    27601 taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg aagatcaata
    27661 cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac tttcgcgaag
    27721 gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg gatcattacc
    27781 aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg attgaaaggc
    27841 agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca atcgacaaga
    27901 aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat ctatctgttt
    27961 tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca tctaagtact
    28021 attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt gttacgccta
    28081 cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct caatacgaaa
    28141 ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg acaaaaacga
    28201 ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac tagcagtttc
    28261 aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa gagatatagt
    28321 gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt ttttcgaaat
    28381 tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt cggagcattc
    28441 gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa ctgaaacagc
    28501 taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt catcacaatc
    28561 aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg caatcagctt
    28621 tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa
    28681 cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa
    28741 gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc agaatctggc
    28801 cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg agatcaaaaa
    28861 agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca cgaatgtagg
    28921 agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag attggaagac
    28981 aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg gggcaattat
    29041 tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct
    29101 gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc caacggctgt
    29161 cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac aagtatcttt
    29221 aataaataac acattttaat aagtagttga acacataact accttacttg caaaggctta
    29281 acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc ggacagtttt
    29341 cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt agaacgcgtc
    29401 ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc actacactaa
    29461 ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg ggattggaca
    29521 aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag aaactaacca
    29581 tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag gagctgttgg
    29641 cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag ccggaatggc
    29701 agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca aattacctaa
    29761 atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa gtccatttga
    29821 attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac atcgatttga
    29881 acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct acgcctatcc
    29941 aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta ggtgcacgta
    30001 gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg gaattagctt
    30061 atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac
    30121 aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta caaacgttga
    30181 gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc
    30241 gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa
    30301 atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat aaagaaactg
    30361 aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg atcctacaac
    30421 gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa gctttcttta
    30481 tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact gagatagatg
    30541 tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca gacttcggtg
    30601 tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca gaacagacag
    30661 aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat caagaagaca
    30721 aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat gagatagcga
    30781 tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt tcatcaaaaa
    30841 gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac tttttctagt
    30901 ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg ttgagatttc
    30961 cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat gggtcgtttt
    31021 aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgttgga tgaaataaaa
    31081 atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta taaaatttta
    31141 cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag tatggggatc
    31201 acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt gatcgacacc
    31261 tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag ccttgaaaac
    31321 ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga atattttggt
    31381 aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt ggctgatgca
    31441 ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga gaatatcatc
    31501 gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa tctggaaaat
    31561 aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc gataactgta
    31621 caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca ccgtgcactt
    31681 tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga gccatctatc
    31741 caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca
    31801 ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct gaaacaagca
    31861 aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt cgtttataag
    31921 gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat catggcaatc
    31981 gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc accaaaacaa
    32041 acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt aaccctttaa
    32101 aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta aagtaggtat
    32161 taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg tagatggaat
    32221 cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact tgttaaaata
    32281 cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt cattcaacgt
    32341 aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac catggggcga
    32401 actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat cagcagctga
    32461 aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt
    32521 tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa cagttatctc
    32581 aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga acgacaaatt
    32641 tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc aaatgactct
    32701 agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt
    32761 tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat tgaacggtaa
    32821 attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag aattagtaac
    32881 agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa
    32941 cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg gtatgactta
    33001 cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca aacaattagt
    33061 taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag tacgtacttt
    33121 agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat agacatgtac
    33181 atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa gtttattcag
    33241 gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac aaaaaagttc
    33301 ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac gacactcgta
    33361 tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa gcaatccttt
    33421 tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct ttagctccag
    33481 ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct gaaactcgcg
    33541 gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc gtattcgaaa
    33601 atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca gaactaggta
    33661 aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca gctcaccgtg
    33721 cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct ggattcttga
    33781 tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga ccaatgattg
    33841 caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac ttgattccta
    33901 aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa gcaaaaggaa
    33961 tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa gaattaatcg
    34021 aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca gaattttcaa
    34081 atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg gctttagaca
    34141 tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa acagttgttt
    34201 ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca atcggtgttt
    34261 gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga gactcagccg
    34321 cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca ggtggcggcg
    34381 caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc aacgataaat
    34441 aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag ggagttttca
    34501 tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg taaacctatt
    34561 atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt tgctgaagca
    34621 gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc tcctgcatta
    34681 ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc agcacaaaac
    34741 tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc tcttgctgat
    34801 ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt ccatgaaaca
    34861 gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac accaatccta
    34921 tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg gatcgaagga
    34981 caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat ggttattgct
    35041 tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat cgctgacgaa
    35101 atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt atcagaagca
    35161 gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata catggcaaaa
    35221 gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc attcttgtca
    35281 ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa atgtttgcac
    35341 taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa aaacaaactc
    35401 aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga tcttagactc
    35461 acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt ttggccgcgg
    35521 aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac gtgacggaga
    35581 caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa acaacatcat
    35641 tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca aagctatgat
    35701 cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt taggtgtttc
    35761 tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc actacctagg
    35821 cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg gcggatcaca
    35881 tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg ctccaacatt
    35941 caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa tcttgaaagc
    36001 tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc ttggttcaaa
    36061 cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg tacctggtaa
    36121 agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag aaaaaggtgt
    36181 ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga tcaaattcta
    36241 cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag acgaaaacga
    36301 ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat tagttggtga
    36361 cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag gaattgctaa
    36421 ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg aagctatcga
    36481 aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg aaacagaaga
    36541 ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa ctggttctct
    36601 atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag accaacttgg
    36661 tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca aataattctt
    36721 ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca cttagttaag
    36781 taatcgaact ctctacttgc tgaaatatca gcaggtagag a
//
LOCUS       DAFELO010000022        35999 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFELO010000022
VERSION     DAFELO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.022"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..560
                     /locus_tag="LP452_001207"
     CDS             <1..560
                     /locus_tag="LP452_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2013944.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="LP452_001208"
     CDS             760..1074
                     /locus_tag="LP452_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2013945.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="LP452_001209"
     CDS             1095..1472
                     /locus_tag="LP452_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2013946.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="LP452_001210"
     CDS             1482..2255
                     /locus_tag="LP452_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013947.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="LP452_001211"
     CDS             2277..3680
                     /locus_tag="LP452_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2013948.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="LP452_001212"
     CDS             3862..5046
                     /locus_tag="LP452_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2013949.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="LP452_001213"
     CDS             5043..5333
                     /locus_tag="LP452_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013950.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="LP452_001214"
     CDS             5330..5551
                     /locus_tag="LP452_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013951.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="LP452_001215"
     CDS             5593..6372
                     /locus_tag="LP452_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBL2013952.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="LP452_001216"
     CDS             6439..6933
                     /locus_tag="LP452_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2013953.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="LP452_001217"
     CDS             6989..7627
                     /locus_tag="LP452_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013954.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="LP452_001218"
     CDS             7699..8094
                     /locus_tag="LP452_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2013955.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="LP452_001219"
     CDS             8078..10531
                     /locus_tag="LP452_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2013956.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="LP452_001220"
     CDS             10528..12555
                     /locus_tag="LP452_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2013957.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="LP452_001221"
     CDS             12552..13574
                     /locus_tag="LP452_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2013958.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..14520
                     /locus_tag="LP452_001222"
     CDS             13591..14520
                     /locus_tag="LP452_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2013959.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14765..14881
                     /locus_tag="LP452_001223"
     CDS             14765..14881
                     /locus_tag="LP452_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL2013960.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14897..16816
                     /gene="tet(M)"
                     /locus_tag="LP452_001224"
     CDS             14897..16816
                     /gene="tet(M)"
                     /locus_tag="LP452_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL2013961.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            16914..17102
                     /locus_tag="LP452_001225"
     CDS             16914..17102
                     /locus_tag="LP452_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL2013962.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(17160..17513)
                     /locus_tag="LP452_001226"
     CDS             complement(17160..17513)
                     /locus_tag="LP452_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013963.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            18044..18466
                     /locus_tag="LP452_001227"
     CDS             18044..18466
                     /locus_tag="LP452_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2013964.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18463..18693
                     /locus_tag="LP452_001228"
     CDS             18463..18693
                     /locus_tag="LP452_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013965.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            19191..19391
                     /locus_tag="LP452_001229"
     CDS             19191..19391
                     /locus_tag="LP452_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL2013966.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            19418..20611
                     /locus_tag="LP452_001230"
     CDS             19418..20611
                     /locus_tag="LP452_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2013967.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(20679..22244)
                     /gene="guaA"
                     /locus_tag="LP452_001231"
     CDS             complement(20679..22244)
                     /gene="guaA"
                     /locus_tag="LP452_001231"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL2013968.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(22303..23226)
                     /locus_tag="LP452_001232"
     CDS             complement(22303..23226)
                     /locus_tag="LP452_001232"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL2013969.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            23422..24027
                     /locus_tag="LP452_001233"
     CDS             23422..24027
                     /locus_tag="LP452_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2013970.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            24335..25351
                     /locus_tag="LP452_001234"
     CDS             24335..25351
                     /locus_tag="LP452_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2013971.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            25492..26793
                     /locus_tag="LP452_001235"
     CDS             25492..26793
                     /locus_tag="LP452_001235"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL2013972.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            26839..27501
                     /gene="deoC"
                     /locus_tag="LP452_001236"
     CDS             26839..27501
                     /gene="deoC"
                     /locus_tag="LP452_001236"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL2013973.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            27522..27920
                     /locus_tag="LP452_001237"
     CDS             27522..27920
                     /locus_tag="LP452_001237"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL2013974.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            27986..29068
                     /locus_tag="LP452_001238"
     CDS             27986..29068
                     /locus_tag="LP452_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2013975.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            29280..30842
                     /locus_tag="LP452_001239"
     CDS             29280..30842
                     /locus_tag="LP452_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2013976.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            30835..31968
                     /locus_tag="LP452_001240"
     CDS             30835..31968
                     /locus_tag="LP452_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013977.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVILLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            31968..32924
                     /locus_tag="LP452_001241"
     CDS             31968..32924
                     /locus_tag="LP452_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2013978.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            33023..34189
                     /gene="deoB"
                     /locus_tag="LP452_001242"
     CDS             33023..34189
                     /gene="deoB"
                     /locus_tag="LP452_001242"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL2013979.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            34213..35031
                     /locus_tag="LP452_001243"
     CDS             34213..35031
                     /locus_tag="LP452_001243"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2013980.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            35048..35755
                     /gene="deoD"
                     /locus_tag="LP452_001244"
     CDS             35048..35755
                     /gene="deoD"
                     /locus_tag="LP452_001244"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2013981.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKKFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11855 a         6241 c         7468 g        10435 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatggtgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    14521 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    14581 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    14641 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat
    14701 aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
    14761 tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca
    14821 taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg
    14881 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
    14941 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
    15001 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
    15061 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
    15121 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
    15181 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
    15241 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
    15301 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
    15361 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
    15421 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
    15481 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc
    15541 tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac
    15601 taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa
    15661 aattgaatat acaaaaaaaa gacaacgtct tgcatatata cgcctttata gtggagtact
    15721 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
    15781 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
    15841 tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac tattgccaca
    15901 gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc
    15961 tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct
    16021 acgatattac gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
    16081 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
    16141 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
    16201 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
    16261 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
    16321 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
    16381 ggattgtaaa atctgtttta agtacggttt atactatagc cctgttagta ctccagcaga
    16441 ttttcggatg cttactccta ttgtactgga gcaagccttt agaaaagctg gaacagaatt
    16501 gttagagcca tatcttagtt ttaaagttta tgcaccacag gaatatcttt cacgggcata
    16561 taacgatgct cccaaatatt gtgcaaatat cgtaaatact caactgaaaa ataatgaggt
    16621 cattattatt ggagaaattc ctgctcgatg tattcaagat tatcgcaatg atttaacttt
    16681 ttttacaaat gggcttagtg tttgtttagc agagctaaaa ggatatcagg ttaccactgg
    16741 cgaacctgtt tgccagaccc gtcgtctaaa tagtcggata gataaagtaa gatatatgtt
    16801 caataaaata acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag
    16861 atgaaatatt ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa
    16921 actacaagtg gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg
    16981 atactatact taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta
    17041 atgttcaaaa aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat
    17101 aatttgagaa atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac
    17161 tagccttcaa tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt
    17221 tctttgtctg taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc
    17281 aaattatctg taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca
    17341 tacagcactt gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg
    17401 tcaatttcaa tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct
    17461 ttaatcgcta aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat
    17521 cacctctata cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat
    17581 atagtttatg agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca
    17641 aatagaaagg tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct
    17701 agaaagtaga taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct
    17761 gtcatctagt tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag
    17821 atatgaccat caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca
    17881 gagaaaacaa tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct
    17941 cttcatttat gcaacaaatc cccctttcgt gtcggggttt agtatgaagg gagataaaga
    18001 agcacatgcc actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt
    18061 tcaagaaacc atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg
    18121 aaaagattac ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat
    18181 agataactac cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact
    18241 atttaaatta tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg
    18301 tcatctttca gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga
    18361 tgtggagatt gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag
    18421 tggactagcc atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca
    18481 agggttccct tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag
    18541 attgttaaac attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa
    18601 tatggaaatt ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc
    18661 atctctaaga ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga
    18721 atcgtatcca cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt
    18781 cagtaggttt tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg
    18841 aatacgtttc ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg
    18901 agcgattcat gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt
    18961 atcgtgataa tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat
    19021 gggtacggct acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt
    19081 tcgattgcta agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt
    19141 ataggtgaat attggagttt tcctagccta taattgggaa aggagtaata atgataacgg
    19201 tcacaaaaaa ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa
    19261 aaaaagcaaa agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag
    19321 atagagtccc tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca
    19381 ggtctgagta gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa
    19441 agcaaagaat ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa
    19501 aattcaaaaa tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa
    19561 actgctattg atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca
    19621 ttatatcttt gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga
    19681 aaatcgctta ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga
    19741 tattgagcct attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc
    19801 ttatgtaaga gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg
    19861 gataaccacc acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat
    19921 tgaattttgg acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta
    19981 ctatcaacac tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg
    20041 tgaagctact gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa
    20101 caaaacactc tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc
    20161 gagcgttcgt catattgtat tagataaatg cacattaact tatttatccg aatggaagga
    20221 agtacaacaa aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca
    20281 aaaacataca ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat
    20341 cagaatccat gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc
    20401 acttatcatt aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg
    20461 acatctttat cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt
    20521 taaagaagca aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt
    20581 gcgaaacaaa gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag
    20641 aaacccttgg tataagagga tttctagctc tcctacaact attcccactc cactgttgct
    20701 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
    20761 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
    20821 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
    20881 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
    20941 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
    21001 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
    21061 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
    21121 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
    21181 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
    21241 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
    21301 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
    21361 ttcaacttgt ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt
    21421 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
    21481 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
    21541 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
    21601 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
    21661 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
    21721 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
    21781 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
    21841 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
    21901 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
    21961 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
    22021 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
    22081 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
    22141 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    22201 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    22261 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    22321 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    22381 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    22441 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    22501 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    22561 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    22621 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    22681 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    22741 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    22801 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    22861 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    22921 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    22981 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    23041 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    23101 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    23161 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    23221 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    23281 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    23341 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    23401 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    23461 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    23521 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    23581 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    23641 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    23701 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    23761 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    23821 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    23881 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    23941 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    24001 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    24061 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    24121 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    24181 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    24241 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    24301 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    24361 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    24421 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    24481 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    24541 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    24601 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    24661 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    24721 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    24781 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    24841 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    24901 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    24961 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    25021 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    25081 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    25141 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    25201 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    25261 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    25321 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    25381 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    25441 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    25501 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    25561 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    25621 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    25681 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    25741 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    25801 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    25861 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    25921 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    25981 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    26041 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    26101 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    26161 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    26221 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    26281 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    26341 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    26401 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    26461 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    26521 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    26581 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    26641 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    26701 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    26761 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    26821 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    26881 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    26941 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    27001 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    27061 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    27121 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    27181 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    27241 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    27301 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    27361 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    27421 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    27481 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    27541 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    27601 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    27661 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    27721 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    27781 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    27841 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    27901 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    27961 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    28021 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    28081 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    28141 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    28201 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    28261 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    28321 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    28381 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    28441 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    28501 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    28561 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    28621 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    28681 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    28741 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    28801 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    28861 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    28921 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    28981 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    29041 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    29101 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    29161 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    29221 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    29281 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    29341 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    29401 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    29461 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    29521 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    29581 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    29641 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    29701 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    29761 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    29821 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    29881 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    29941 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    30001 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    30061 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    30121 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    30181 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    30241 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    30301 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    30361 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    30421 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    30481 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    30541 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    30601 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    30661 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    30721 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    30781 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    30841 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    30901 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    30961 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    31021 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    31081 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    31141 ccagatctgc caaaagttat tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    31201 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    31261 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    31321 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    31381 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    31441 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    31501 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    31561 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    31621 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    31681 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    31741 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    31801 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    31861 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    31921 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    31981 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    32041 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    32101 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    32161 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    32221 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    32281 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    32341 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    32401 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    32461 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    32521 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    32581 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    32641 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    32701 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    32761 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    32821 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    32881 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    32941 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    33001 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    33061 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    33121 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    33181 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    33241 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    33301 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    33361 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    33421 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    33481 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    33541 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    33601 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    33661 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    33721 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    33781 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    33841 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    33901 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    33961 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    34021 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    34081 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    34141 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    34201 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    34261 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    34321 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    34381 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    34441 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    34501 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    34561 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    34621 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    34681 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    34741 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    34801 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    34861 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    34921 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    34981 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    35041 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    35101 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    35161 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    35221 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    35281 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    35341 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    35401 aaaattccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    35461 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    35521 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    35581 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    35641 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    35701 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    35761 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    35821 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    35881 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    35941 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttata
//
LOCUS       DAFELO010000023        35719 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFELO010000023
VERSION     DAFELO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.023"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..705)
                     /locus_tag="LP452_001245"
     CDS             complement(<1..705)
                     /locus_tag="LP452_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2013982.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="LP452_001246"
     CDS             1393..1551
                     /locus_tag="LP452_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2013983.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2849
                     /locus_tag="LP452_001247"
     CDS             1623..2849
                     /locus_tag="LP452_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2013984.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="LP452_001248"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="LP452_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL2013985.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="LP452_001249"
     CDS             complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="LP452_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL2013986.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4308..4775)
                     /locus_tag="LP452_001250"
     CDS             complement(4308..4775)
                     /locus_tag="LP452_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL2013987.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4895..6433)
                     /locus_tag="LP452_001251"
     CDS             complement(4895..6433)
                     /locus_tag="LP452_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL2013988.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6513..7199
                     /locus_tag="LP452_001252"
     CDS             6513..7199
                     /locus_tag="LP452_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL2013989.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="LP452_001253"
     CDS             complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="LP452_001253"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2013990.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="LP452_001254"
     CDS             complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="LP452_001254"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL2013991.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8685..9815)
                     /locus_tag="LP452_001255"
     CDS             complement(8685..9815)
                     /locus_tag="LP452_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2013992.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9815..11998)
                     /locus_tag="LP452_001256"
     CDS             complement(9815..11998)
                     /locus_tag="LP452_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL2013993.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11995..13020)
                     /locus_tag="LP452_001257"
     CDS             complement(11995..13020)
                     /locus_tag="LP452_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2013994.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13065..13892)
                     /locus_tag="LP452_001258"
     CDS             complement(13065..13892)
                     /locus_tag="LP452_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2013995.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13889..14956)
                     /locus_tag="LP452_001259"
     CDS             complement(13889..14956)
                     /locus_tag="LP452_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL2013996.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14982..15818)
                     /locus_tag="LP452_001260"
     CDS             complement(14982..15818)
                     /locus_tag="LP452_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2013997.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15815..16684)
                     /locus_tag="LP452_001261"
     CDS             complement(15815..16684)
                     /locus_tag="LP452_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2013998.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16754..18037)
                     /locus_tag="LP452_001262"
     CDS             complement(16754..18037)
                     /locus_tag="LP452_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL2013999.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18051..19736)
                     /locus_tag="LP452_001263"
     CDS             complement(18051..19736)
                     /locus_tag="LP452_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL2014000.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20011..21036
                     /locus_tag="LP452_001264"
     CDS             20011..21036
                     /locus_tag="LP452_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2014001.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="LP452_001265"
     CDS             complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="LP452_001265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL2014002.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="LP452_001266"
     CDS             complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="LP452_001266"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL2014003.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29708..30676
                     /locus_tag="LP452_001267"
     CDS             29708..30676
                     /locus_tag="LP452_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2014004.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30694..31599
                     /locus_tag="LP452_001268"
     CDS             30694..31599
                     /locus_tag="LP452_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014005.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31596..32402
                     /locus_tag="LP452_001269"
     CDS             31596..32402
                     /locus_tag="LP452_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2014006.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32538..33527
                     /locus_tag="LP452_001270"
     CDS             32538..33527
                     /locus_tag="LP452_001270"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL2014007.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33844..34368)
                     /locus_tag="LP452_001271"
     CDS             complement(33844..34368)
                     /locus_tag="LP452_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL2014008.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34655..>35719
                     /gene="scm"
                     /locus_tag="LP452_001272"
     CDS             34655..>35719
                     /gene="scm"
                     /locus_tag="LP452_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL2014009.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10272 a         7539 c         6612 g        11296 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt
     2761 ctacaacgag aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct
     2821 ttcagagagg attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga
     2881 aaaagacact tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta
     2941 acgtttagag aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac
     3001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3601 cactttgtct gcattgatga cgatgacgaa atcaccagta tccaaatgag gtgtgaaagt
     3661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     3901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     3961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4141 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4201 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4261 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4321 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4381 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4441 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4501 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4561 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4621 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4681 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4741 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4801 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4861 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     4921 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     4981 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5041 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5101 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5161 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5221 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5281 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5341 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5401 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5461 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5521 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5581 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5641 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5701 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5761 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5821 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     5881 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     5941 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6001 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6061 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6121 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6181 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6241 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6301 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6361 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6421 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6481 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6541 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6601 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6661 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6721 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6781 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6841 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     6901 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     6961 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7021 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7081 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7141 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7201 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7261 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7321 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7381 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7441 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7501 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7561 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7621 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7681 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7741 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7801 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7861 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     7921 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     7981 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8041 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8101 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8161 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8221 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8281 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8341 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8401 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8461 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8521 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8581 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8641 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8701 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8761 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8821 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     8881 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     8941 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9001 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9061 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9121 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9181 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9241 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9301 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9361 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9421 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9481 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9541 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9601 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9661 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9721 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9781 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9841 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     9901 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     9961 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10021 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10081 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10141 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10201 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10261 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10321 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10381 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10441 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10501 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10561 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10621 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10681 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10741 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10801 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10861 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    10921 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    10981 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11041 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11101 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11161 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11221 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11281 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11341 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11401 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11461 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11521 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11581 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11641 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11701 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11761 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11821 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    11881 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    11941 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12001 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12061 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12121 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12181 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12241 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12301 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12361 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12421 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12481 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12541 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12601 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12661 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12721 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12781 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12841 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    12901 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    12961 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13021 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13081 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13141 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13201 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13261 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13321 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13381 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13441 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13501 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13561 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13621 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13681 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13741 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13801 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13861 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    13921 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    13981 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14041 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14101 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14161 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14221 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14281 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14341 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14401 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14461 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14521 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14581 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14641 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14701 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14761 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14821 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    14881 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    14941 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15001 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15061 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15121 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15181 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15241 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15301 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15361 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15421 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15481 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15541 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15601 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15661 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15721 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15781 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15841 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    15901 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    15961 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16021 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16081 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16141 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16201 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16261 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16321 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16381 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16441 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16501 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16561 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16621 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16681 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16741 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16801 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16861 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    16921 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    16981 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17041 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17101 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17161 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17221 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17281 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17341 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17401 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17461 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17521 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17581 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17641 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17701 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17761 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17821 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    17881 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    17941 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18001 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18061 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18121 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18181 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18241 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18301 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18361 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18421 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18481 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18541 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18601 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    18661 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18721 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18781 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18841 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    18901 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    18961 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19021 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19081 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19141 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19201 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19261 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19321 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19381 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19441 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19501 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19561 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19621 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19681 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19741 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19801 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19861 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    19921 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    19981 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20041 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20101 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20161 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20221 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20281 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20341 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20401 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20461 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20521 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20581 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20641 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20701 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20761 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20821 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    20881 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    20941 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21001 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21061 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21121 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21181 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21241 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21301 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21361 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21421 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21481 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21541 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21601 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21661 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21721 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21781 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21841 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    21901 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    21961 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22021 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22081 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22141 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22201 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22261 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22321 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22381 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22441 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22501 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22561 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22621 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22681 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22741 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22801 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22861 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    22921 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    22981 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23041 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23101 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23161 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23221 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23281 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23341 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23401 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23461 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23521 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23581 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23641 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23701 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23761 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    23821 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    23881 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    23941 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24001 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24061 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24121 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24181 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    24241 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24301 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24361 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24421 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24481 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24541 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24601 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24661 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24721 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24781 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24841 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    24901 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    24961 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25021 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25081 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25141 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25201 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25261 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25321 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25381 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25441 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25501 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25561 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25621 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25681 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25741 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25801 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25861 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    25921 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    25981 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26041 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26101 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26161 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26221 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26281 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26341 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26401 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26461 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26521 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26581 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26641 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26701 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26761 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26821 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    26881 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    26941 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27001 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27061 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27121 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27181 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27241 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27301 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27361 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27421 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27481 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27541 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27601 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27661 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27721 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27781 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27841 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    27901 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    27961 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28021 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28081 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28141 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28201 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28261 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28321 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28381 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28441 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28501 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28561 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28621 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28681 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28741 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28801 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    28861 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    28921 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    28981 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29041 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29101 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29161 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29221 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29281 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29341 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29401 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29461 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29521 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29581 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29641 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29701 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29761 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29821 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    29881 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    29941 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30001 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30061 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30121 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30181 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30241 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30301 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30361 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30421 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30481 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    30541 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30601 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30661 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30721 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30781 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30841 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    30901 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    30961 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31021 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31081 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31141 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31201 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31261 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    31321 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31381 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31441 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31501 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31561 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31621 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31681 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31741 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31801 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31861 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    31921 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    31981 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32041 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32101 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32161 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32221 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32281 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32341 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32401 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32461 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32521 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32581 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32641 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32701 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32761 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32821 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    32881 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    32941 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33001 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33061 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33121 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33181 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33241 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33301 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33361 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33421 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33481 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33541 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33601 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33661 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33721 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33781 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33841 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    33901 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    33961 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34021 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34081 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34141 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34201 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34261 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34321 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34381 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34441 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34501 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34561 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34621 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34681 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34741 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34801 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34861 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    34921 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    34981 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35041 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35101 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35161 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35221 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35281 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35341 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35401 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35461 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35521 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35581 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35641 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35701 tgatagctca gattcatca
//
LOCUS       DAFELO010000024        35018 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFELO010000024
VERSION     DAFELO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.024"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(55..2652)
                     /gene="adhE"
                     /locus_tag="LP452_001273"
                     /gene_synonym="adhC"
     CDS             complement(55..2652)
                     /gene="adhE"
                     /locus_tag="LP452_001273"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL2014010.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2957..3337)
                     /gene="yajC"
                     /locus_tag="LP452_001274"
     CDS             complement(2957..3337)
                     /gene="yajC"
                     /locus_tag="LP452_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL2014011.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3434..4579)
                     /gene="tgt"
                     /locus_tag="LP452_001275"
     CDS             complement(3434..4579)
                     /gene="tgt"
                     /locus_tag="LP452_001275"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL2014012.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4797..5495
                     /locus_tag="LP452_001276"
     CDS             4797..5495
                     /locus_tag="LP452_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL2014013.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5663..8302)
                     /locus_tag="LP452_001277"
     CDS             complement(5663..8302)
                     /locus_tag="LP452_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2014014.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8921..9952)
                     /gene="queA"
                     /locus_tag="LP452_001278"
     CDS             complement(8921..9952)
                     /gene="queA"
                     /locus_tag="LP452_001278"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL2014015.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10145..11320
                     /locus_tag="LP452_001279"
     CDS             10145..11320
                     /locus_tag="LP452_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2014016.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11324..11965
                     /locus_tag="LP452_001280"
     CDS             11324..11965
                     /locus_tag="LP452_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014017.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11962..12879
                     /locus_tag="LP452_001281"
     CDS             11962..12879
                     /locus_tag="LP452_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2014018.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12876..13547
                     /locus_tag="LP452_001282"
     CDS             12876..13547
                     /locus_tag="LP452_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014019.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14088..14576)
                     /locus_tag="LP452_001283"
     CDS             complement(14088..14576)
                     /locus_tag="LP452_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL2014020.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14777..15043)
                     /locus_tag="LP452_001284"
     CDS             complement(14777..15043)
                     /locus_tag="LP452_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2014021.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15043..15306)
                     /locus_tag="LP452_001285"
     CDS             complement(15043..15306)
                     /locus_tag="LP452_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2014022.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            15782..15967
                     /locus_tag="LP452_001286"
     CDS             15782..15967
                     /locus_tag="LP452_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2014023.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16109..17932)
                     /locus_tag="LP452_001287"
     CDS             complement(16109..17932)
                     /locus_tag="LP452_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL2014024.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18530..21235)
                     /locus_tag="LP452_001288"
     CDS             complement(18530..21235)
                     /locus_tag="LP452_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL2014025.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21541..22488)
                     /locus_tag="LP452_001289"
     CDS             complement(21541..22488)
                     /locus_tag="LP452_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL2014026.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22678..22947
                     /gene="rpsN"
                     /locus_tag="LP452_001290"
     CDS             22678..22947
                     /gene="rpsN"
                     /locus_tag="LP452_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL2014027.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(23123..23485)
                     /locus_tag="LP452_001291"
     CDS             complement(23123..23485)
                     /locus_tag="LP452_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2014028.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(23496..24617)
                     /locus_tag="LP452_001292"
     CDS             complement(23496..24617)
                     /locus_tag="LP452_001292"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL2014029.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(24633..24983)
                     /locus_tag="LP452_001293"
     CDS             complement(24633..24983)
                     /locus_tag="LP452_001293"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL2014030.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(25113..26624)
                     /locus_tag="LP452_001294"
     CDS             complement(25113..26624)
                     /locus_tag="LP452_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2014031.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(26946..28298)
                     /locus_tag="LP452_001295"
     CDS             complement(26946..28298)
                     /locus_tag="LP452_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL2014032.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(28451..29527)
                     /locus_tag="LP452_001296"
     CDS             complement(28451..29527)
                     /locus_tag="LP452_001296"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL2014033.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFNMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            29723..31468
                     /locus_tag="LP452_001297"
     CDS             29723..31468
                     /locus_tag="LP452_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL2014034.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(31748..32185)
                     /locus_tag="LP452_001298"
     CDS             complement(31748..32185)
                     /locus_tag="LP452_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL2014035.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(32169..33542)
                     /locus_tag="LP452_001299"
     CDS             complement(32169..33542)
                     /locus_tag="LP452_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014036.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(33864..34352)
                     /locus_tag="LP452_001300"
     CDS             complement(33864..34352)
                     /locus_tag="LP452_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014037.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(34374..34976)
                     /locus_tag="LP452_001301"
     CDS             complement(34374..34976)
                     /locus_tag="LP452_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2014038.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9919 a         7475 c         6466 g        11158 t
ORIGIN      
        1 tttccaagtt ctttttcatc tattttctta tcttttttct acgctttctt ttgcttatcc
       61 gttgtaagca tcaatgatga tctgtttcaa ttcagagatc aatggttctt tcggattagc
      121 tgtcgtacat tggtcttcgt aagctaattc agccatacgg tctacggttg tatctagtgt
      181 ttcttgtgtg actccttgag cttttagact catgttgatg ccgacagatt ttcctaattc
      241 ataaacagcc gttgctaaag cttcgactaa ttcctctgtt gtttcgcctt tcagacctaa
      301 gaattttgcg atatctgcgt aatctgtatc tgcacggaaa tagtcatatt tagggaacat
      361 cgcatgtttt tgtggatctt ttgcattata gcggataata tgcggtaaaa gaatcgcatt
      421 tgtccgtcca tgagggatcc cgtattcccc gccgatttta tgtgcgactg agtgacaaat
      481 acctaggaat gcgttagcaa acgccatacc agccattgta gaagcgttgt gcattttttc
      541 acgtgattcc atatcaggtg tttttactga tttttctaaa tagtcaaaga ctaacttgat
      601 tgcttgtagg ctcaatccac gtgtataatc tgaagccatg actgaaacat aggattcgat
      661 tgcatgtgtt aacacatcca tcccggtatc tgctgtgaca gatgctggaa ctgacatgac
      721 gaattgagga tcgacgattg cgacatctgg cgtcaatgca taatctgcta acggatattt
      781 cacatgtgtt tcactgtcag tgatgaccgc aaatggcgtt acttcagaac cagtccctga
      841 tgttgtcggg atacatacga attgtgtttt ttctggtttg tcgatcttat atgtccgttt
      901 acggatatcc aagaattttt gttttgcacc aaagaaagat gtgtctgggt gttcatagaa
      961 catccacatt cccttagctg cgtccattgc agaaccgcct ccaagagcga ttactgtatc
     1021 tggcttgaag tctgccatca gtttcgttcc tgcgtatacg gtatttgtcg atgggttcgg
     1081 ttcaacatct gagaagactt cgatcttcac atcattttta cgtcgtgcca atacttcacg
     1141 aactgtatcg cagtaaccga attggaccat accagggtca caaacgatca tgacacgttc
     1201 aacgttttcc attttctcta agtacaatag agagttcttt tcaaagaaga tttttggcgg
     1261 taatttgaac cattgcatgt tatttctccg tttcgctaca gttttgacat tgattaagtt
     1321 tacagcagag acgttacgag aaacagagtt cttaccataa gaaccgcatc ccaaagtcaa
     1381 tgacggaatc atttcgttat agatatcacc gatgccgcct tctgcagatg gtgtattcac
     1441 taaaatacga caagctttca ttcgaagacc gaatttgaca tgcaactctt cgttttctga
     1501 atggataact gctgtatgtc ctaatcctcc aagattcaac ataccttcac acaaatcaaa
     1561 cgcatggtcc gtgttgttag ctttgatcat ggcaagaact ggagaaagtt tttctcttga
     1621 tagcggataa tctgggccta cgccttccaa ttcagcaatc agcattttcg ttccttttgg
     1681 tacactgatt cctgccaatt ttgcgatttc catcgcagaa tgtccaacga ttgatggatt
     1741 gaccgcatat tttccttcgt tcatcactgc atcttctaat ttttgcaatt catctggttt
     1801 tacgatataa acttggtgtg cttggaattc tgcttttact gctgcataga tttctttatc
     1861 cacgatcaca gcttgttcag aagcacaaat cattccatta tcaaatgttt tcgaaacgat
     1921 caaatcgttc actgcacgtt taatttttgc tgttttttca atataagctg gtacgttacc
     1981 tggacctaca cctaaagctg gttttcctgt agagtaagca gacttgacca ttccagctcc
     2041 acctgtcgct aagacgatcg cgatacctgg atggttcatt agcatagaag tcgcttcgat
     2101 cgaaggatgt tcgatccatt ggatacaatt ttcaggagct ccggcagcga tagcagcatc
     2161 acgtaccact cgtgctgctt ctgcagaaga tttttgggca cttggatgga aagcaaaaac
     2221 gattggattt cttgttttca acgcgatcat tgatttgaaa atcgttgtgg aagtcggatt
     2281 cgttgttgga gttactccgc aaaccacacc gactggttca gcaatttcaa tcaacccttt
     2341 ttggacatct tcattgatca caccaactgt tttatcatgt ttgatattat gccaaatata
     2401 ttcggacgca tacatatttt tgattgcttt atcttcgtaa acaccgcggc ctgtttcctc
     2461 tacagccatc ttcgctaatg gcatatgttg atccaaagct gccattgcca tttcatgcac
     2521 gatatggtct actttttctt gatcaaagct ttccatttct ttcaatgctg catttgcttt
     2581 ttctgccaaa tcatcaatca ttgcttgtac gtcaattgtc ttcgtttttt ctttttctat
     2641 tactttagcc atcattatcc tcctaatggg tcatggttat ttgttaaata attcacatgt
     2701 atatcataca atatttattt ttctatgtaa acactttttt gtgatttttt tcacagttat
     2761 ttttaaatat aaaattgctt tatttacagg tgtttgtata gaaaaatata taaagttatt
     2821 tattattatt tatatgacac acccgattat tcaatattga gcaatatatc ccatgctctt
     2881 atctctttct tttttgaata aaaaatgagg taggacatct ttctgcccta cctcgtcttt
     2941 aaataagctt tgatacttat ttttcttcct tctcttctgt ctcttccagc gtttctgttg
     3001 tttcagtttt tacttcaact gtctcagctg gttcttctgt tgtttcaaca gtagtcgttg
     3061 catcatttgt tacgacagtc cctggtttga ccgttttgat tgctgcacga tcgtactcaa
     3121 ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc attgtcgatt tctgaaacaa
     3181 ctccatgtaa tccgccgatc gttacgactg aatcacctgg tttcatgcta tctaacaagt
     3241 tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat aaaaaaccac attgctaata
     3301 gcgcgataaa catcacaagc attggtaatg ttcccatttt tttcacctca ctaattctgt
     3361 atctacaaaa aacactttag cagaaaaatg ctgataacac tagtttttta cttaattttt
     3421 aaaagatttt cctttagaag ctttttgcgt tttctttatt gaacccgtat tcttcaaaaa
     3481 aggcttctct aaattcaagc aaattatcat ccatgattgc ttgacgtact tgtttcatta
     3541 gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg aatcccaaat gtttcatcac
     3601 atttgatcaa atggcggata taggcacgtg tatagttttt acatgtataa caatcacatt
     3661 tctcatcaat tggacggaaa tcatgggcat atttcgcatt tttcactact agacggcctt
     3721 gagaagtcat acatgtgcca tttcgagcga ttcgagtagg caagacacag tcaaacatgt
     3781 ctactccacg gataacacca tcgatcaatg aatcagcagc accgacaccc atcaagtaac
     3841 gtggtttgtt ttcggggatc aacggtgtag tgaattctaa tacacgattc atttctgatt
     3901 taggttcccc gacagataac ccgccgattg agtaaccagg gaaatccatc ccgactagat
     3961 ctttcgcgct ttgacgacga agatcttcaa accctgctcc ttggatgatt ccaaataatc
     4021 cttgacggtc tggattagca tgagctttca atcctcgttc tgcccatcga gacgtacgtt
     4081 ctactgattt tttcacgtag tcgtaacttt catcgaatgg tggacattcg tcaaaactca
     4141 tcatgatatc agagcccaat ttattttgga tgttgattgc tttttctggt gataaaaaca
     4201 tacgcgaacc gttcaagtga tttttgaaat gcacgccttc ttcttcgata ttccgcatat
     4261 cacttaatga aaaaacttga aagccgcctg aatctgttaa aatcggttga tcccagttca
     4321 taaatttatg cagtccgcca gcttcttcta ctaagtcttc accagggcgt agccataaat
     4381 gatatgtatt acttaaaata acgccagcac ccatttcttt caactcttct ggggacattg
     4441 tttttactgt agctaatgtt cctactggca tgaacattgg tgtggggaaa gttccgtgtg
     4501 gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc tttcttgatt aaacgatagc
     4561 gaatggcagg ttgcgtcatt catttcccta ctttctatat aaaatatctt gacaactccc
     4621 accatcataa tcgtttcaag tgctttttgc aagttccctc ggcttccttc tactttattt
     4681 aatattcttt tttttatcct ttttttatga ataacaaaaa aaagaaaaac attatgacag
     4741 aaatattttt tgatacatgg tatattggtg ttattaaaga aaaggaggga aaaatcatgc
     4801 aatcttctgc agaattaaaa cgccaagcaa aagagagtct aaaaggacgt tggggacaag
     4861 ctgttttact aaatctaatc cctacattga ttggtattgc tactattctt cttattatga
     4921 ttcctattat cttgtttgcc accaccatat atgatggatc agcgagcgtc tcagaagcga
     4981 caacttcagg aggcggtagt tcaggaggcg gtggcggaat tatttccagc atcattagcg
     5041 ctttgttcat gagcggtatt tcttggactt atcttgatat tttacgtgga caaaaagatc
     5101 agatcgaacc attcaaagat gcatttcgtg gattcaaagg tttgtttatc ggcggagtga
     5161 tcagcttagc tgtgctgatc tcgatcttta caacattatg gggtttgcta ttagttattc
     5221 ctggaatcat taaaagctat tcttattctc aaagctattt tatttactac gatatcgtcc
     5281 aagaaacagg acaaacacct aaagtactgg atacgatcac tgcaagtcga aaattgatgg
     5341 atggttataa aggacaactt ttctggttag atctttcatt tatcggctgg catatcttag
     5401 cattagcaac tgctggtatt ggttatctat ggctcagccc atacatttct gcaacaaaag
     5461 cagcatttta tgaacaattg ccaaaagata tttaaaagag agtgtgactt gtgaccgagg
     5521 gggtggcagg agaacaccgt ttatcgagag gcagtaaaag aaacattcag cccattagca
     5581 aaaaggaggt gtgacaacat tcatgtcaca cctccttttt gtatagttcc agtaaaaaga
     5641 aaatcaactt agaaacagtt aatcattcct cttcttctgc ttccggtaat cgatggaact
     5701 tgatcagttc attcaccgcg attggtacga tagataaaat ccctaccaag aaccagtgat
     5761 tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa gcaacagccg
     5821 ttgtaatagc taatgccagt aacgccattt ctaccaaagc tttattggtc gctaaattga
     5881 ctttgaagat cgattggctg ctgcgtacat tgaacacatg cagaatcgat gaataagcta
     5941 agatcaagaa tgcaacagtt tgaccgacat gctgacttgc gtctacccat gggctgatag
     6001 tatcgacata actacctaaa taataaccaa acaaggttac gatggtgaag acccctgcat
     6061 ttatggcaat tttttgcaat aaacctcgag caaagatccc ttcatttttc ggaatgggtg
     6121 gctgatccat gatgccgtgt tccgctggct ctcttcctaa agcaaatcct ggaagtccat
     6181 cagcaactac attgacaaac agtagttgca cagctgtaaa tggtgctccc catcctaaca
     6241 gcatcgcaat caaaacaata aagatctccg aaatattaca gcttagcaaa aagttgatag
     6301 ctttacgaat attctgataa accgaacgtc cttgactgac agcgtctacg atcgtcgcaa
     6361 agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt cctgtgatcc
     6421 ccatggcaca cccaacgtta cttgctttca acgctggtgc atcattcaca ccatccccag
     6481 tcatcgctac gactgcaccc gtacgttgcc aagatttgac gatccggatc ttgtcttctg
     6541 gcgttacccg agcatagaca gagagagctt tgacacgcgc atctaattct tcgtctgata
     6601 attgatgcag ttctgatcct gaaagtgctt ctgacggatc attcaagata cctaattctt
     6661 tcgctatggc actggcagtc accacatgat ctccagtgat catgactgtt ttgatgccag
     6721 cctttttcgc tcgcttgatt gctcctttac tttctggacg tggtggatcg atcattccga
     6781 tcagaccaat caaacgtaag tctttttcca gtgcttcaga agaaatatct tgcggttctt
     6841 cgtcataagt agcataacca acagcaatca ctctcagtgc ccgtttacca aagcgatcat
     6901 taacgatcgc cgcttgttct acatcgcctg aactaaagcg cggagcaaga acatcgaatg
     6961 ctcctttggt gattgagatg tatttttcgc ccatttggtg aactgttgtc atcatttttc
     7021 gttcagaatc aaaaggaagc tcgtttactc gtggatattt ttcttctaat tctgcctttg
     7081 tatgataatt ctcttcaaca gcccttacga tggctacttc tgttggatta cctttgataa
     7141 caaggtcgtc atcttcttgc tcgaccgtca catctgtaca tagtgcagcc attttcaata
     7201 tttccatcgc gctgttcgtc attgaatctt cgatattcgt tacttcatct tctttgctcc
     7261 atacccggcg gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct gagcagataa
     7321 cattagcggt tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca tgtttcctcg
     7381 ccatcttctg cacaccgtat gcaagtgtca acgtaacaat aacagtcaag gtttccggta
     7441 cagctgcaac agccaaggaa atcgaagtca tgaacatctc aaggagtggt tctccttgca
     7501 gttcaccaat cacaaatact aaagccgcag ccgctaaagc gatcatactg attcgtttgc
     7561 ctagctgatt caatcggacc tgtaaaggtg ttttttgcat agtattttcg ttcaaaagat
     7621 ccgcgatctt acccatttct gtagccatgc caatggcagt gacgatcgct ttcccacgac
     7681 ctgcagcaac cgtacatcct ttaaatacca tattaagttg gtcacctaat gactcatctt
     7741 cttcagcaat aaatgctgca tctttttcaa ctgcttcact ttctccagtc aacgccgatt
     7801 cttctacacg taattgtgat gcttcgatca aacgggcatc ggcagaaata gcatcaccgg
     7861 actctaaaac aagaatatca cccactacca gttgctctga ctcaacagtg gtcactttgc
     7921 cgtctcgtat gacagttgcc gtttgtttat tcatatcttg caaggattcc agtgcttttt
     7981 ctgcgtttcc ttcttggaca atcgccaata ccgagttgat cacaacgata gcgatgatca
     8041 gcagtccttc aaataaatcc ccatgttctg tagcaaatgc ggtataaaag gaaatcaccg
     8101 ctgctacaag aaggatgatc gttgtaaaat cggacaagct tcgaaagaat tttttgatca
     8161 tcgattcttt cggcgcctca tcgaatttat tcaatccctg cttttttcgc cgttcatcca
     8221 cttcttgtga agacaaccca gtatctggat ctgcttctgt taccttcttt acttcttcta
     8281 ttgtctgctt gtattcttcc atcctattcc tcctttaaaa tcttcgtaca aaaaaaggta
     8341 cttattttac tataacatcc tttttttatt tacttcaaat aaagggctta tcgattattt
     8401 gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa ggatgctatt
     8461 atatagatgt aaggaaggag gataccatca aatagacaga agaatttctc gatagaacag
     8521 tgtaagcaaa caaagttgtt agaagtacag gtttcaccca gacatgcgtg tagagtacaa
     8581 acctataaat agtgaatgcg tatggatgtt tggatcacga agtatccgaa acaagaagta
     8641 atgcgatacg taacaattga ataacgtaaa cgtctgaaaa ttgaatagga acaagagatg
     8701 tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt taacatgaag aaattcaaca
     8761 ccgtgctttg catgtatgat ggaaagttac aatcatttct gtttatttga atccttccga
     8821 ggtacaagaa agccgagctt atcgagagct cggctttttc tttgtcataa aatctttgta
     8881 aagctttttt gttattcaaa actagcggct tttcataacc ttatttcacg aacatcgcat
     8941 ctccaaaact aaagaaacga tagcgctctt caatcgcatg ctggtaagca gataaagtca
     9001 attctcttcc tgcaaaggca ctaactaaca tgactaaagt cgattttggc aaatggaaat
     9061 tagttgagaa agcttctacc actttgaatt catagccagg cgtaatgaaa atatcagtcc
     9121 atccgctgtc tgctttgatt tctccattaa atttcgttcc aatcgtttct aatgttcgaa
     9181 ttgaagttgt tccgacagcc acgattttcc cgccattttt acgtacttgg ttcaatcgct
     9241 cagccgcttc ttccgtcaaa cggtagaatt cactatgcat atggtgttct tcaattttat
     9301 ctacgctgac aggtcggaat gtacctaacc ctacatgtag tgtcagatag actaattcaa
     9361 caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa atgcaatcca gctgttggcg
     9421 ctgcagcaga accattttct ttcgcataga cagtttgata gcgatctggg tcttccagac
     9481 gttctttgat atatggaggc aatggcattt ctcccaatga ttcaagaatc tctagaaaaa
     9541 ttccttcata ctcaaagtca atgatccgtc cgccgtgttc caattcttct ttcactactg
     9601 cttgcaatct gccatcacca aaagagattc ttgtcccaac tttggcacgt ttcgcaggtt
     9661 tgatcaatgt ctcccacgta tctccttgtg tattcgttag aagaaggact tctaaatgcg
     9721 caccagtatc tggtttttct ccatataaac gagcaggcag aactcgtgta ttattcataa
     9781 ctagcgcatc accaggctgt aactcttcaa tgatttcatg gaaatgttta tcttccatct
     9841 ctccagtttt ccgatccaaa acaagtaaac gagaatgatc gcggtctttt aatggtgttt
     9901 gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc tgttgttaac atatcgtcac
     9961 tcactttcta tcatgtgctt catcattcta tcatgttttt cgttctgttg gcaaaatcag
    10021 cactttttca cttctatttt ttgttttcct aaataaaaaa actaccctat ttgtattttt
    10081 tcattgtttt aggcatacta gtaatagata gttcttcatt gaaaatagaa aaagaggtgg
    10141 caccatgatt gaatttcaaa atgtgtcaaa aatttataaa ggtggaaaag tcgctgtaga
    10201 ggacgtcaat ctttcatttg aaaagggtga gttcatttgt tttatcggta cgagcggaag
    10261 cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact gatccaacac aagggaaaat
    10321 tttgattgat ggaaaatcca tccaagaaat tgatccagtg gaattgcgac gtcagatcgg
    10381 ctatgttatc caaagtattg gattgatgcc tcacatgacg atccgtgaaa atatcactct
    10441 tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat aaaacagctg aaaaaatgat
    10501 tgatcttgtg gaattgcccc gtgagatgtt agatcgctac ccacatgaat tatctggtgg
    10561 tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg aatcaagata tcatcttgat
    10621 ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat tccttgcaag accttgtcaa
    10681 agatttacaa gagcgtttgg gaaaaacgat cgtatttgta acccatgaca tggatgaagc
    10741 gattaaacta gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac
    10801 accagaaaat attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga
    10861 tcgcctacta caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc
    10921 aatcacgatt acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg
    10981 tgtagatacg ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga
    11041 aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt
    11101 tttttacgta aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg
    11161 gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc
    11221 atcattagtg gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt
    11281 tgcagagtca gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca
    11341 tcagcacggt tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc
    11401 tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa
    11461 agctgccacg attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt
    11521 attggcattg atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt
    11581 tatttattca ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg
    11641 gaattatcgg gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt
    11701 tgaattgccc attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt
    11761 gattgcatgg gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt
    11821 tagtggactg aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat
    11881 tttagcacta ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc
    11941 acttagagag gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct
    12001 cttcttatac ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc
    12061 atttcgatca caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga
    12121 atggttgaac attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact
    12181 atcaaccatc aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga
    12241 acagatttaa cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct
    12301 accgtgaaag atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt
    12361 gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa
    12421 gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc
    12481 aatcgtaaag gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg
    12541 atattaccta tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt
    12601 gttcttggct actctacgga tggacggatc gccagttatg atctagtcat gttggaagat
    12661 gatcgtcaat tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct
    12721 gatacgcctc atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa
    12781 atgcaggaat tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa
    12841 cgtttcttac aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg
    12901 tctttcaaca atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta
    12961 tccgtcactt cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg
    13021 ttgggatcat gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata
    13081 tcatccagac gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg
    13141 gagtcaatgt cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata
    13201 cgtataccgg gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga
    13261 tgactgctag acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg
    13321 caggcataag aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg
    13381 gtgctggtgg tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga
    13441 ttattttagc aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag
    13501 gtattttgga aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa
    13561 aacaaaagct tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta
    13621 aggcaataat ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta
    13681 tttatccatt ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg
    13741 actaagtttt tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca
    13801 tggaattcat accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca
    13861 attcttacat cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc
    13921 gatgccttca gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt
    13981 ttatacaatc gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag
    14041 cttctcgcca tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat
    14101 ccgtcgcaac tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc
    14161 aattgatcga tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt
    14221 gatgcattgc cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc
    14281 gtttgagtga cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg
    14341 acactggtat tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct
    14401 gggaaccaat aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct
    14461 gtttctcgat aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc
    14521 gttgccactg ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt
    14581 cttcctcctt gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc
    14641 ttcaaaggtg gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg
    14701 gttttagaac aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag
    14761 gttggctctt ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat
    14821 tttcctcatc aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc
    14881 cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca
    14941 atgtctgaat cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat
    15001 ccactttcgc agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc
    15061 aatatcatcg atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa
    15121 agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat
    15181 caatacagca ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt
    15241 tacctctttc aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt
    15301 agccattgtc tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa
    15361 aaaaatacaa taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa
    15421 gacccctttt tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg
    15481 tagccaaacg taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag
    15541 cctgtgagtc tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat
    15601 tgatatcttg aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga
    15661 ttttggccgt caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt
    15721 catgttcact atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa
    15781 aatggcactg agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta
    15841 tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg
    15901 tacattattt ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta
    15961 cagctaaata ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag
    16021 agggcacctt tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga
    16081 gggtctgttt ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga
    16141 caacgatatt ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga
    16201 gaatattctg ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt
    16261 tttgtttcgc tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg
    16321 agcgataaga agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg
    16381 cttcgatttc tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat
    16441 aatcaccgat cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga
    16501 ctaatacaag aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa
    16561 gctgttctgc tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg
    16621 gcataatgat gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga
    16681 ttgcatatga gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc
    16741 cttctttttt ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc
    16801 cgatagagta taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga
    16861 tcgaacctag cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt
    16921 gatacatgtg aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag
    16981 aaaaaccggt gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat
    17041 acaaaatacc atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt
    17101 caggaacgat cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga
    17161 tcaatgccat catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg
    17221 gtacagcatt actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc
    17281 gtaaaatcaa agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca
    17341 atggcacagc acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa
    17401 ggataaatgt gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat
    17461 tcagcatcat cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg
    17521 ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca
    17581 tgtagtcgat caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac
    17641 tgctgacgac atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata
    17701 gcgttaatga aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg
    17761 ctgcagcaga caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg
    17821 ataacgcatc agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat
    17881 tttcagcaga ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa
    17941 ctcctttaca cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg
    18001 ttatagtacg ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa
    18061 cagaaaaata tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt
    18121 atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt
    18181 ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca
    18241 ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag
    18301 cttgcatctg cggtaataag ctcaaccaaa ttaaaaacat aagaagctgt ttttatttgg
    18361 tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa
    18421 tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat
    18481 ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa
    18541 tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga
    18601 ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg
    18661 tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa
    18721 atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc
    18781 cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat
    18841 cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa
    18901 atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg
    18961 taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt
    19021 gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa
    19081 cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag
    19141 ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa
    19201 agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga
    19261 cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg
    19321 gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt
    19381 tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt
    19441 ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata
    19501 ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat
    19561 tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg
    19621 ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt
    19681 ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag
    19741 aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat
    19801 taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa
    19861 cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat
    19921 ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca
    19981 ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt
    20041 tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg
    20101 ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg
    20161 taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt
    20221 ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat
    20281 cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg
    20341 tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga
    20401 tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca
    20461 ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca
    20521 cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga
    20581 ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat
    20641 ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata
    20701 ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg
    20761 aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat
    20821 attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat
    20881 cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt
    20941 cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt
    21001 gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag
    21061 caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg
    21121 catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga
    21181 aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc
    21241 tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc
    21301 ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct
    21361 cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga
    21421 aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat
    21481 tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc
    21541 ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg
    21601 tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc
    21661 tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct
    21721 tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc
    21781 atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat
    21841 acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt
    21901 tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt
    21961 actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga
    22021 cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa
    22081 atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca
    22141 actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta
    22201 agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga
    22261 accaatctct gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt
    22321 tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc
    22381 ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc
    22441 aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc
    22501 cctttttctt taagtttacg tttatgtctt tattttgaca aaaaaaatgt cttttcgttt
    22561 tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat
    22621 ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg
    22681 gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct
    22741 gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta
    22801 gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac
    22861 atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt
    22921 ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc
    22981 gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct
    23041 ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg
    23101 ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac
    23161 taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt
    23221 ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt
    23281 ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt
    23341 ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa
    23401 taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa
    23461 aataaatatc tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc
    23521 cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc
    23581 tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga
    23641 aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag
    23701 tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa
    23761 ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg
    23821 attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct
    23881 ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct
    23941 actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg
    24001 ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct
    24061 ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa
    24121 ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca
    24181 ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc
    24241 gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct
    24301 gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc
    24361 aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt
    24421 gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca
    24481 tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca
    24541 acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg
    24601 tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa
    24661 gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc
    24721 acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg
    24781 atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat
    24841 tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt
    24901 cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca
    24961 tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag aacactctca
    25021 ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt
    25081 tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat
    25141 ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta
    25201 taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt
    25261 ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct
    25321 ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt
    25381 gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta
    25441 tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct
    25501 ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt
    25561 tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct
    25621 tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata
    25681 tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc
    25741 gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt
    25801 tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc
    25861 aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca
    25921 tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga
    25981 tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg
    26041 aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt
    26101 ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac
    26161 atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg
    26221 ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag
    26281 ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg
    26341 tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg
    26401 ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca
    26461 aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat
    26521 gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact
    26581 gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat
    26641 ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta
    26701 ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca
    26761 ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt
    26821 tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt
    26881 ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca
    26941 gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg
    27001 ccttttagca taatactatc tgttgctgca agattttgtt tgatagcatc aatcaattta
    27061 ttcttttctt tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct
    27121 tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata
    27181 tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct
    27241 aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc
    27301 atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt
    27361 agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt
    27421 atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa
    27481 cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt
    27541 ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt
    27601 tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat
    27661 ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac
    27721 ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc
    27781 ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt
    27841 tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt
    27901 ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg
    27961 gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa
    28021 taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt
    28081 tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct
    28141 tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg
    28201 gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg
    28261 cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt
    28321 tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt
    28381 tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct
    28441 ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc
    28501 ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga
    28561 gtgggtacat attgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt
    28621 catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt
    28681 aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa
    28741 tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt
    28801 ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga
    28861 tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg
    28921 ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca
    28981 tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt
    29041 ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac
    29101 cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata
    29161 cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt
    29221 ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg
    29281 gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat
    29341 gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt
    29401 cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg
    29461 aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa
    29521 tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag
    29581 cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa
    29641 cgagcgtcat ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg
    29701 ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat
    29761 cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga
    29821 acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct
    29881 ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga
    29941 tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct
    30001 ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg
    30061 gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc
    30121 taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat
    30181 caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg
    30241 ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca
    30301 aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa
    30361 aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga
    30421 aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc
    30481 aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga
    30541 tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag
    30601 ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac
    30661 ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg
    30721 gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa
    30781 agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga
    30841 tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat
    30901 tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa
    30961 tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt
    31021 gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca
    31081 ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt
    31141 ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg
    31201 gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag
    31261 gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg
    31321 gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg
    31381 gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat
    31441 ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa
    31501 gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt
    31561 tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac
    31621 ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc
    31681 ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg
    31741 gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg
    31801 taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac
    31861 gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca
    31921 ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt
    31981 cattcttctt aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt
    32041 agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga
    32101 taaccacgtc aagaccatag gattctccta attttatgac atcttctttt actggagaac
    32161 catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt
    32221 actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca
    32281 ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc
    32341 atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct
    32401 ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt
    32461 tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag
    32521 acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc
    32581 aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt
    32641 gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg
    32701 tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt
    32761 agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa
    32821 acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt
    32881 tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat
    32941 aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa
    33001 cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag
    33061 cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag
    33121 atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga
    33181 aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt
    33241 gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca
    33301 ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg
    33361 actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc
    33421 caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta
    33481 cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac
    33541 atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag
    33601 gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata
    33661 agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa
    33721 aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt
    33781 gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat
    33841 ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt
    33901 tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta
    33961 taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt
    34021 gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt
    34081 actttattta gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca
    34141 gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt
    34201 tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga
    34261 ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc
    34321 agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa
    34381 tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccat
    34441 ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct
    34501 actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt
    34561 ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat
    34621 aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc
    34681 ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg
    34741 cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac
    34801 agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg
    34861 ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga
    34921 ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat
    34981 attccccaca tcttttttca tttattaaaa ctgcctcc
//
LOCUS       DAFELO010000025        34522 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFELO010000025
VERSION     DAFELO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.025"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(133..306)
                     /locus_tag="LP452_001302"
     CDS             complement(133..306)
                     /locus_tag="LP452_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2014039.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="LP452_001303"
                     /pseudo
     CDS             754..3116
                     /locus_tag="LP452_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="LP452_001304"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="LP452_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL2014040.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="LP452_001305"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="LP452_001305"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL2014041.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="LP452_001306"
     CDS             5704..6489
                     /locus_tag="LP452_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2014042.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="LP452_001307"
     CDS             6502..7353
                     /locus_tag="LP452_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL2014043.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="LP452_001308"
     CDS             complement(7477..7929)
                     /locus_tag="LP452_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014044.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="LP452_001309"
     CDS             complement(8394..8762)
                     /locus_tag="LP452_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL2014045.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="LP452_001310"
     CDS             8912..9394
                     /locus_tag="LP452_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL2014046.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="LP452_001311"
     CDS             9431..10441
                     /locus_tag="LP452_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL2014047.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="LP452_001312"
     CDS             complement(10556..11269)
                     /locus_tag="LP452_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014048.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="LP452_001313"
     CDS             complement(11459..12049)
                     /locus_tag="LP452_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL2014049.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="LP452_001314"
     CDS             12533..12760
                     /locus_tag="LP452_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL2014050.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="LP452_001315"
     CDS             12776..13942
                     /locus_tag="LP452_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL2014051.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="LP452_001316"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="LP452_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL2014052.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="LP452_001317"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="LP452_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL2014053.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="LP452_001318"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="LP452_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL2014054.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="LP452_001319"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="LP452_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL2014055.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="LP452_001320"
     CDS             complement(19155..19820)
                     /locus_tag="LP452_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014056.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="LP452_001321"
     CDS             19796..19969
                     /locus_tag="LP452_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014057.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="LP452_001322"
     CDS             complement(20014..20751)
                     /locus_tag="LP452_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2014058.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="LP452_001323"
     CDS             complement(20753..22255)
                     /locus_tag="LP452_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL2014059.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="LP452_001324"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="LP452_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL2014060.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="LP452_001325"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="LP452_001325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL2014061.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="LP452_001326"
     CDS             27597..28862
                     /locus_tag="LP452_001326"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL2014062.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="LP452_001327"
     CDS             28866..29597
                     /locus_tag="LP452_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL2014063.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="LP452_001328"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="LP452_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL2014064.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPEF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="LP452_001329"
     CDS             30613..31611
                     /locus_tag="LP452_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL2014065.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="LP452_001330"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="LP452_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL2014066.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="LP452_001331"
     CDS             33123..33773
                     /locus_tag="LP452_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014067.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="LP452_001332"
     CDS             33960..>34522
                     /locus_tag="LP452_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL2014068.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11453 a         5832 c         7324 g         9913 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg aaatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccagaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DAFELO010000026        32392 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFELO010000026
VERSION     DAFELO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.026"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(440..973)
                     /locus_tag="LP452_001333"
     CDS             complement(440..973)
                     /locus_tag="LP452_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL2014069.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1837..2589)
                     /gene="xth"
                     /locus_tag="LP452_001334"
     CDS             complement(1837..2589)
                     /gene="xth"
                     /locus_tag="LP452_001334"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL2014070.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2702..3622
                     /locus_tag="LP452_001335"
     CDS             2702..3622
                     /locus_tag="LP452_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2014071.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3695..4630
                     /gene="murB"
                     /locus_tag="LP452_001336"
     CDS             3695..4630
                     /gene="murB"
                     /locus_tag="LP452_001336"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL2014072.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            4928..6406
                     /locus_tag="LP452_001337"
     CDS             4928..6406
                     /locus_tag="LP452_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014073.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            6683..8743
                     /locus_tag="LP452_001338"
     CDS             6683..8743
                     /locus_tag="LP452_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL2014074.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            8826..9395
                     /locus_tag="LP452_001339"
     CDS             8826..9395
                     /locus_tag="LP452_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014075.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(9570..10376)
                     /locus_tag="LP452_001340"
     CDS             complement(9570..10376)
                     /locus_tag="LP452_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014076.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            10514..12028
                     /locus_tag="LP452_001341"
     CDS             10514..12028
                     /locus_tag="LP452_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL2014077.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(12431..13414)
                     /locus_tag="LP452_001342"
                     /pseudo
     CDS             complement(12431..13414)
                     /locus_tag="LP452_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(13419..14066)
                     /locus_tag="LP452_001343"
     CDS             complement(13419..14066)
                     /locus_tag="LP452_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2014078.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(14151..14783)
                     /locus_tag="LP452_001344"
     CDS             complement(14151..14783)
                     /locus_tag="LP452_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2014079.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(14799..15350)
                     /gene="coaC"
                     /locus_tag="LP452_001345"
     CDS             complement(14799..15350)
                     /gene="coaC"
                     /locus_tag="LP452_001345"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL2014080.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(15347..16084)
                     /locus_tag="LP452_001346"
     CDS             complement(15347..16084)
                     /locus_tag="LP452_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL2014081.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(16081..17070)
                     /locus_tag="LP452_001347"
     CDS             complement(16081..17070)
                     /locus_tag="LP452_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL2014082.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            17328..17870
                     /locus_tag="LP452_001348"
     CDS             17328..17870
                     /locus_tag="LP452_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2014083.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            17887..18972
                     /locus_tag="LP452_001349"
     CDS             17887..18972
                     /locus_tag="LP452_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014084.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            18984..19790
                     /locus_tag="LP452_001350"
     CDS             18984..19790
                     /locus_tag="LP452_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014085.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            19791..20618
                     /locus_tag="LP452_001351"
     CDS             19791..20618
                     /locus_tag="LP452_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014086.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            20615..21688
                     /locus_tag="LP452_001352"
     CDS             20615..21688
                     /locus_tag="LP452_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2014087.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            22116..24812
                     /locus_tag="LP452_001353"
     CDS             22116..24812
                     /locus_tag="LP452_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2014088.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            24889..25377
                     /locus_tag="LP452_001354"
     CDS             24889..25377
                     /locus_tag="LP452_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2014089.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            25389..25883
                     /locus_tag="LP452_001355"
     CDS             25389..25883
                     /locus_tag="LP452_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2014090.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            25904..26713
                     /locus_tag="LP452_001356"
     CDS             25904..26713
                     /locus_tag="LP452_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2014091.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            26710..27531
                     /locus_tag="LP452_001357"
     CDS             26710..27531
                     /locus_tag="LP452_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2014092.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            27582..29681
                     /locus_tag="LP452_001358"
     CDS             27582..29681
                     /locus_tag="LP452_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL2014093.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            29678..30796
                     /locus_tag="LP452_001359"
     CDS             29678..30796
                     /locus_tag="LP452_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL2014094.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            30835..31713
                     /locus_tag="LP452_001360"
     CDS             30835..31713
                     /locus_tag="LP452_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2014095.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            31859..32056
                     /locus_tag="LP452_001361"
     CDS             31859..32056
                     /locus_tag="LP452_001361"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL2014096.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT        10416 a         5572 c         6687 g         9717 t
ORIGIN      
        1 aacacacgaa gggactgtga caaaagtcgt gtcgcagtcc attttttttg aataaacggt
       61 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
      121 gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tggtaaacgg
      181 tgtccaaaag cttgcatctg cggtaataag tccaaccaaa tgaaaaatat gagaagctat
      241 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccaataa
      301 acggtgttct cctgccagct cctcggtcac cagtcacaat atttaataaa catgagaagc
      361 tattttttga aattgctcct tgtgactcgg agtgaaacgg caggctcacg acctctttgt
      421 gtgtttcata agtattcttt tactttatag tcacaagttt tttcaatgga tctgtcccga
      481 attttttttc ttgataaagc aggatttccg cacatgcacg actatcttcc aatgcatcat
      541 ggtgatgatc taattggatg ttcaaatttt cgcacacagt gttcaatcga tgatttggaa
      601 attcaggaaa caggcgacgg ctacttttca ctgtacataa agaaagataa ttaggctgtt
      661 caagatgata ataatctaag catcccgcta agacctttgt atcaaaagca gcattatgtg
      721 caacgactaa acgatttttt tgaaaatatg gttggatagt ttgccagact tctgggaatt
      781 tcggcgcatt gcgtacgtct tcttgatgga ttccgtgaat ttggacattt cgccagaaaa
      841 aaggagtctc aggctgtatc aatgtataga attcatcaac gatctggcta ttttttacca
      901 tgactagcgc aagagaacaa gcactataag gctgatgatt ggcagtttca aaatccatgg
      961 caacaaaatt catttctgta aaacctcctt gggaaattac tgtaacatac ttttgaaaaa
     1021 agcaaataaa tttaacaaaa aacatagaat tttttatgta atatttgttg agaaaagcta
     1081 gtttttgaac tcattccttg tgatttggag ttagatgggg atcatgactt ctcggtatta
     1141 agtcaaattt atgcaaagtt gtgaggaacc attttcaaaa aaatttctta cgactcgaaa
     1201 gtaggcgact ttttcacaac ttctccacta aacggtgttc aatcagctga ccctcggtat
     1261 tagctcaaaa aatagcaaaa tatgaggaac tattttgcca tttgtttctc taatactcag
     1321 gtcaggacgc tgatttcaca acttctcaat aaacggtgtt caaaagcttg catctgcggt
     1381 aataagtcca accaaacgaa aaatatgagg aactattttt gtttggctgt tcttattact
     1441 tgatgcagtg cagctttttc acaacctctt aataaacggt gttcaaaagc ttgcatctgc
     1501 ggtaataagc ccaaccaaac gaaaaatatg aggaactatt tttgtttggc tgttcttatt
     1561 acttgatgca gtgcagcttt ttcacaacct cttaataaac ggtgttcaaa agcttgtatc
     1621 tgcggtaata agcccaacca aacgaaaaat atgaggaact atttttgttt ggctgttctt
     1681 attacttgat gcaggacagc tttttcacaa tctctcgata aacagtgttc aaaagccagt
     1741 ttctcggtat tatgccaaat ttatgcaaaa tgtggaaagc cattttcaca aaatttttct
     1801 taatactcga aactaaacga ctttttcaca acctctttat ttcaaatcta attctactgg
     1861 acaatgatca ctgcccataa tgtccgtatg gatctttgca tcagctagtc ctggctctaa
     1921 atctttgctc gtcaaaaagt aatcgatccg ccaaccagca ttgtttttcc ttgaattgaa
     1981 tcggtagttc caccaagagt agacaccttc ctgggttgga taaaaataac ggaatgtatc
     2041 gataaacccg ttatccaaca gtcgagaaag agccgctctt tcttctggag tgaatccagc
     2101 atttttttga ttcgttttcc agtttttcaa atcaatcttt tgatgagcga catttagatc
     2161 cccacacacg atgactggtt tttgtttttt tagattttcc aaataattat aaaaggcttc
     2221 ttcccattct aatcggtaat ctaaacgttt taattcgctt tgagaatttg gcgtgtaaca
     2281 ggttacaaga aagaaatcgg aatattccaa agtaatcaga cggccttctg tatcatggat
     2341 atcgataccc attccgtaag tggcattcaa tgctggtttt ttagcaaaaa tcgctgtgcc
     2401 ggaatagcct tttcgttccg cgtaattcca atattgataa taacctggaa gatctaaatc
     2461 aatttgtcct gcttggagtt tcgtttcctg cagacagaaa aaatcggcgt ctaactcatg
     2521 aaaaacttct aaaaaatttt tatttacgat ggcacgaagc ccgtttacgt tccaagagat
     2581 aaatttcaat aaaagcacac tccttcgaat taatttcctt acctttgcga tactattgta
     2641 cctcatttta gtaaaaaatg gtgtaagatg aaagtaacta aatgatccag gaggaaagta
     2701 gatgaagatt ggtgtaatcg gtgtagggaa tatcgcagaa aaagcatatt taccaactta
     2761 tgcgaaaaaa caaggaacac ttgattttta ttttgcaaca agaaataaag aaacaaaaga
     2821 aagaattcaa aaagagtacg ggttccaaca tctatatgaa acgatcgatg agcttttaga
     2881 agaaaaaatc gaagcatgta tgatccacgc agcaacaaaa gtacattatg aattagccaa
     2941 aaaatgtcta gaaaatggta tccatgttta tattgacaaa ccactgagtg tccatctaaa
     3001 tgaagttcgt gaattgcaag aattagctga taagaaccaa ataattttga tgatcggttt
     3061 caaccggcgc tttgctccta tggtagaaga attgaaacgt atcccagata agcggttgat
     3121 ccacctgcaa aaaaatcgaa tagcggcaaa agaaacgaca gagttcgtcc tttatgattt
     3181 gtttttacat cttattgata cagctgtgta tttattagat gaacccattt tgagaacaaa
     3241 ataccagata agagaaacaa aggaattttt ggaatatgcc attttacaat tagaaacaca
     3301 aaatcaaacg gctattttaa cgatggattt agcaagtggc gctaatatag aaaaatatca
     3361 agtaacaagt aaaaatggta cttatgaagt aaacgatctg acccagcttg tcattcaaga
     3421 tgctgatgga aaaaaagtga aagaatttgg cgactggaca aacacgctag tcaaacgcgg
     3481 gtttgagcca atggtagagg ccttcttttc tcagatccgt ttaggtaaat cggaaacaga
     3541 attgcttaaa caaaaagaag ttgtaagaag tcatgaatta tgtgaggaag tgcttaaaga
     3601 tcactaccgt catcaacttt gaacaagact ggcaagattg tattaagcgt gatagaataa
     3661 tgaggacgtt tgagaaaaga aaggaaaatc ctcagtgaat aaagaagaaa tagttgaaag
     3721 cttagccacg attactatat tgtttgatga accattgatg aactatacgt ttacgaaaac
     3781 aggtggacca gctgatgtcc ttgcattccc gaaaaaacaa gaagaagtaa agcaaatcat
     3841 cgattattgc cgaatccatg atataccttg gatggtatta ggaaatgcaa gcaatctgat
     3901 cgttcaagat ggcgggattc gcggtgtagt catcatgctg actgaaatga aacagattca
     3961 tgtcaaagga acgatggtca ttgcggaagc aggtgcctca ctgattgata cgacttatgc
     4021 agctttggca gaatcattga ctggatttga atttgcttgc ggcatcccgg gaagtgtcgg
     4081 tggagcggtt tatatgaatg caggtgcgta tggcggagag atcaaagatg tttttgctga
     4141 agtggatctt ttgcttgaag atggaacatt gaaaactttg acaaaagacg aaatggtatt
     4201 ttcttatcgt cacagtaaag tccaagaatt acgtgctatc gtattggaag ctcgattcag
     4261 tttgcaaaca ggggattatg aagcaatcaa agcaagaatg gatgagttga ccgaattgcg
     4321 tcaatcaaaa cagccattag aatacccttc atgcggaagt gtctttaagc gcccagtagg
     4381 acactataca ggtcagctga tccaacaagc aggattgcaa ggcctgaaat ggggcggtgc
     4441 acaagtctct gaaaaacatg ctggattcat cgtcaatatc gatcatgcga cagcgacaga
     4501 ttatattgaa ttgattgcgc acatccaaga agtaatcaaa gaaaaatttg atgtgtcttt
     4561 agaaacagaa gtgcggatca ttggagaaaa acaagcaata tcagaaacaa gcagaagttt
     4621 agccaattaa taagtaatgg aacttaaaat aagtaaaaga gcaggacggt ggccgtcctg
     4681 ctcttttttg tggttgttta cgcaaatggc gcaaatcttg tacattttaa cacaataaaa
     4741 caaaaaatat agaatatttc ctcataaatt tttccatttt ttgcaatatt ctcaaaaaaa
     4801 agataaaaaa tgagaataac tgatgaaaaa acaagcataa tccatcataa aatcagtata
     4861 ggtgaaaaga tgaactttca tctgcatatc aagtaattgg tatgaggcat tttttgaggt
     4921 gtaggatatg aagttggatt accttataga agaggaagat agagaaaaac tgctgctagc
     4981 tagatattta gaatttgaga aaaagaatta tgtagtgctg cctaagttat atgaaacatt
     5041 aggtttgtct aaatttaaaa caagaaatta tatcatagaa ttaaataaag aatttactga
     5101 attcaaaaca aagccagaga tccaaatttc agaagaagca gagatccatg tcgaacgtct
     5161 gaatttgacg gatatcaaaa atttaagaag aatctacttt caacgatcaa aaattgctcg
     5221 actgttaacg gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag agaatcattt
     5281 tttaagcata tcaagagcct atgtaaaaag aagagaatta gtctcttttc ttgaacagca
     5341 tcaaataaag ttaaagaaaa acatattagt tggggaagaa ataaacatac ggaacgtatt
     5401 atttggggta tatttcgggg tctttaatgg tttttctatg ccatttagag aaagcgtcat
     5461 gaatgaagca aagagtatcg tgcaatattt tgaatatctg ttcaatttaa aactgtctgc
     5521 aacaaaacga atcaaattca atttgctaac cactattagt ttgttgaggg cctatgggaa
     5581 tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt aagagctcag aaggaatgaa
     5641 gatcatcaag gatttatcac atatttcttt tcttcatatc cctaaagagc agatcattga
     5701 tgagatttct tatatcatgt tgtttttgat cacggaagaa gaatttgaat tagatggcgc
     5761 atttggactt gcggaagctc attttcatga tatccacatg attagtcaaa aaatgtcagt
     5821 ggaattgatt gatcaattac cattttcgat tgaaatctct gaagaagaaa gactatatat
     5881 acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga tattcgactt
     5941 tgagatgact agctttacgt ctaaaaagca gctacagttt ttcttcgaga cgtatccaat
     6001 attcagtaca gcaatccaaa aggtcataca agagaataaa cgctatttct tgggaaaaga
     6061 tgaacgtttc atgattcgct tattttatga ttatctcttt ttattggtcg gtatttgtcc
     6121 ggtatcatat tttgaagcac cgattcatgt ctgtattgat ttttcgcaag gatctgctta
     6181 taccgattac attatttctc aagtgaaagg atttaaaaat tacaatattg tgattgagag
     6241 tcgaatcact tctcgtacga atatttattt atctgattgt ttacttgaaa aatttaataa
     6301 agaacagata atttggaaaa atccaccgac tccagatgac tgggaatatt ttggaaatac
     6361 catcatcaga gaaaaaaata gacatttaac tgaaaaaatc agctagtatt tatggatggc
     6421 agctttgtct cacatattta ttagctgatt tatgtttttt tcaaagaaaa ggtaactaga
     6481 tataactaga tataactaga tatattactt aacaaaatta aaaagtacat aagttattat
     6541 tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag tccatcttct
     6601 tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa
     6661 gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca
     6721 cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat
     6781 gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac
     6841 gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac
     6901 tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta
     6961 taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt
     7021 atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc
     7081 tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat
     7141 aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga
     7201 aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag
     7261 gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa
     7321 tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat
     7381 tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat
     7441 caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga
     7501 atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac
     7561 agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac
     7621 atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac
     7681 tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat
     7741 gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc
     7801 aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat
     7861 gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc
     7921 agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat
     7981 accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa
     8041 caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga
     8101 tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg
     8161 tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca
     8221 gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagaca
     8281 agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa
     8341 agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa
     8401 taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat
     8461 tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga
     8521 tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc
     8581 caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg
     8641 tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt
     8701 atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt
     8761 tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata
     8821 gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac
     8881 aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag
     8941 atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag
     9001 cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt
     9061 tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat
     9121 caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa
     9181 aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg
     9241 acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa
     9301 aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa
     9361 aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt
     9421 attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc
     9481 ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa agtattgtca
     9541 ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg aataaaattc
     9601 ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa tacactgaaa
     9661 acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat tcccacgtct
     9721 aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg actttcaatg
     9781 attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact taactccaca
     9841 ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa tttatctgct
     9901 gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa ggaaacaact
     9961 gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa ataaacaagg
    10021 gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt atcccacatt
    10081 gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa aataaaagat
    10141 aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc cagaaaataa
    10201 gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca gtgttggttc
    10261 cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata gattccgttt
    10321 gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa
    10381 taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca aagctatatg
    10441 acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt ataaggagaa
    10501 aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg cacttcttgc
    10561 tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta gtacgaagga
    10621 atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa aagaaatgaa
    10681 agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag cagctatcga
    10741 agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg ccggaggaaa
    10801 cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg
    10861 aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg gaggaacgaa
    10921 tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg attggctaga
    10981 tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg aaaagaggac
    11041 acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt tattgaataa
    11101 cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca ttaagaaaaa
    11161 tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa aagaggtcac
    11221 cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa
    11281 atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa ctggagatgg
    11341 tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa tacaaattca
    11401 tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg gagaaggagc
    11461 aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa gggataaagt
    11521 atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg accaaggcgt
    11581 gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca caactggaaa
    11641 gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag caaaaacagt
    11701 tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc gtgccacagc
    11761 catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac caggaatcca
    11821 ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata aagaaaacca
    11881 gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg gaaacaaccg
    11941 gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg gcatacaagc
    12001 agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat
    12061 cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg aagaggttgt
    12121 gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
    12181 ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt attcggaaaa
    12241 agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    12301 ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
    12361 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttgat
    12421 cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg tttgcttcca
    12481 tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg attggccgaa
    12541 tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg agctctgctt
    12601 tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt ttcccaatca
    12661 atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc aagacccaac
    12721 gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg atgaaatcca
    12781 aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga aagtcttgtt
    12841 ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt ttttcttctg
    12901 atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg atcgctgctg
    12961 ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt aattttccaa
    13021 gctagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca gcagaaacat
    13081 agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca tattcggcat
    13141 aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg gagatatcac
    13201 ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag ggaggtttca
    13261 aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta tggactttga
    13321 tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct aatacatcta
    13381 cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt tgcccattct
    13441 tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt acgagaaata
    13501 tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt gatcgttttt
    13561 gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc gttagtcaat
    13621 gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc atctgcataa
    13681 tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc atctgcataa
    13741 acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat cactgttttg
    13801 attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc agcaaaaatc
    13861 aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt taaatcaaaa
    13921 gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc tctcacccct
    13981 gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc aatttgtccg
    14041 tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa tctttccatt
    14101 caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta tcagttagta
    14161 agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt cgcaagacct
    14221 gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc tgaataaaca
    14281 gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa gtagatcata
    14341 ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg
    14401 gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata aggaaccacc
    14461 ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc atgttctgtt
    14521 ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat aactgtgcta
    14581 cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa
    14641 acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg accgataggg
    14701 ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat cgcagttaat
    14761 gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct ttcattaaga
    14821 gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt tccaaaatct
    14881 ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga tctcaatgtt
    14941 tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc aggggcaatc
    15001 aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc gtctgcaatg
    15061 ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa caagtcagct
    15121 tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat gacgtctgta
    15181 tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg tgtgctgctt
    15241 ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt aagatcagcg
    15301 cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat tttgattcct
    15361 ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga gcttcttcca
    15421 ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt tccgtcaaat
    15481 catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg actgccagaa
    15541 gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct tttggctgcc
    15601 attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca agatgctcgg
    15661 tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca aaccaattgt
    15721 ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct tctgaaaaac
    15781 taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg tagtgttctt
    15841 ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc gtataaatcg
    15901 ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg
    15961 tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct gtcgaatgat
    16021 ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg attagaatct
    16081 tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag caagtagttt
    16141 agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt catctgcaat
    16201 ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta cattgacaga
    16261 atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg cgtttagttt
    16321 gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca atggaagaag
    16381 actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct gttttcctaa
    16441 agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag cacctagact
    16501 ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact ggaatcctag
    16561 tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag taacgacaac
    16621 ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca tagcagtaaa
    16681 ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta gaggaactaa
    16741 ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga cgccaagccc
    16801 aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac tttggacgac
    16861 agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct gaccagactc
    16921 gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta ccaagacttc
    16981 attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa caggttcctt
    17041 tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa aattttcttt
    17101 tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag acaaaaatta
    17161 ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga aatattcagc
    17221 aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa tacgagtggt
    17281 agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg gaaattggtg
    17341 agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta ggcgaacgca
    17401 cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca ccatcaatgg
    17461 aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt tttcatcaag
    17521 aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat ttcgatgaag
    17581 aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg
    17641 tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca ttatctgaaa
    17701 cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg gtctatgtgg
    17761 caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta aggaatcatc
    17821 tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa tatagggggt
    17881 aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta tgatgatgaa
    17941 acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac actgcttggt
    18001 ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac cgaagctaca
    18061 gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa taagcgacaa
    18121 gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt tgataacgtt
    18181 gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat
    18241 gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga aatgtctggt
    18301 ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa agtattgcta
    18361 ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca gtatgaactg
    18421 cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga ccaagaagaa
    18481 gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt
    18541 acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt tgttggggag
    18601 agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt tggtaagcaa
    18661 tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt tttacgccca
    18721 gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt cgatactcag
    18781 ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg
    18841 gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt tgagccagaa
    18901 gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg tcttgacagc
    18961 tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc gtcccttata
    19021 ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat caatcttttt
    19081 ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg tcggggacat
    19141 cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt ttcacgctga
    19201 tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa ttatggctga
    19261 tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca tttattggga
    19321 ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc gggccgaaac
    19381 agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag attccattta
    19441 tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc agtgctagta
    19501 gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg ttatcattaa
    19561 atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta ttcatgctga
    19621 ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa cagcatttct
    19681 tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc attgctatgt
    19741 tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat
    19801 ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca ccgatcttct
    19861 atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca ggatttactt
    19921 gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta ttgaatacgt
    19981 tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt ggggctatgg
    20041 gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg aataacatct
    20101 tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt acactactag
    20161 gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc attccaatcg
    20221 tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc gatgcggcta
    20281 gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct tatctttctc
    20341 caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat tttgcggtta
    20401 catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat tctcgtgcaa
    20461 gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg ttctccatga
    20521 tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa
    20581 acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat ctctgttttt
    20641 aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag
    20701 tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata tcgatcctag
    20761 tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa catttgattc
    20821 taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca tcgctatacc
    20881 aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat tagatcattc
    20941 gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt ttgatccaga
    21001 taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata acgataaatt
    21061 ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat taaaagacag
    21121 tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata gtttaggcta
    21181 ttctttgaat agtaagaata tggcacagct gacagaagca gcaaataaat tgaatcattt
    21241 aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga
    21301 agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg aaaatgaaca
    21361 tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata tcgttatgcc
    21421 aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc tagaaccgga
    21481 aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag aagcaaaagc
    21541 gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg acacgatctc
    21601 ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata atgacctatt
    21661 cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg ggacttatca
    21721 acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc ttctaccaaa
    21781 agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt ttttctacca
    21841 gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta aatctaagat
    21901 tgaaaaagtt acagaaggat aaagtaattg gaaactatgc aaatatactt aacccttctg
    21961 taatctatta ataaaactaa tatatgaatc cagttcaata tttgtagcta tgaaaaaaca
    22021 taaaaatcat attagataaa aactaaattg gtagataaat tattttgtca gatatctgag
    22081 gcaaatattc gactatttgt gtttcaaagt aaaaattgga atacaaatat agaatagaca
    22141 tattgcagcc gatagtttat tatgctttta gacacattaa actggaggaa aaaatgaaaa
    22201 agagcgctaa agatcgagtt tttgatgagc tgatacgact aacagggaat atgtcttctg
    22261 tcactgccga gaaaatttcg aagaatctag gtatctctcg tcagaatgcc agccattatt
    22321 tgacgagatt agtggaagat aaaaaggtcg agaaattacc gggaaagcct gttttgtgga
    22381 agcctttaga tgaatatgcg gttgtagata atacggagca aataaacgaa gcctttcatt
    22441 ctgtagttgg tcatgacgga agtctgagag aggtcattca aaaatgtata gccgctgtca
    22501 aatatccgcc taacggactc agtgtattaa taaatggagc cacgggtgtt ggaaaaagtt
    22561 ttttagccac aaaaattttt gaatacgcta tccatgagca aattattgaa aaagatgcac
    22621 catttgctat cttaaactgt gccgattatg cggacaatcc cgaactgctt tcggcaacac
    22681 tgtttggata caagaagggg gcctttacgg gggctgaaaa ggataccgat ggtttattgg
    22741 caacggccaa caacggctat ctttttctcg atgaggtcca tcgcctatcg aaagaaaatc
    22801 aagaaaagct ctttctattt attgatactg gaaactatcg tccagtcggt gaaaatgtaa
    22861 attggcattc tgctaaggtt cgttttattt tcgccacgac ggaaaaaggt gaaaattatt
    22921 tattagacac ctttgatcgg cgtattcaaa ttagtgttat gttgccaact tttgaagatc
    22981 gccccattcg agaacgatta gagctgattc aattattttt tcaaaatgaa gcggatgttt
    23041 tacaaaaaga tattattgtt tcaagagaag ccttgtcttt aatgttatct catcccttta
    23101 gtgggaatat tggtaagctg aagaatatca ttaaaatcag ttgtgcggat gtgtatagtc
    23161 gaacacaaga agaaccatta acgattggga aaaatgagat cttgattcag ttaaatatgc
    23221 tggaatccga agtagaaagc ttgccgattg ctagtttatt gataaagaaa aatcaaaaga
    23281 taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat tttggaaaaa attactcagc
    23341 tgataaatga aggtaatttc aatgaaaatt tttcttcgat aaagaatgaa gtacagcatt
    23401 tggtgcatca cattgataag gattcttttt tatcaatgag tagaaaattg atgattcaga
    23461 tgtttagaaa ggtctgggta tccgttttag gaaaaacgta cggattgacg acgagtggac
    23521 ctgttgcaga aattattagc agatcctatg tcatttcccc aagctataag acagacttaa
    23581 ccgggataaa tcgtacatta gctgcaaatc tacctcggac gacttactta tgtaatcagt
    23641 tcttggagca tttaccaccg ctctcagaaa aggacgagaa ctttctaaga ctgcttattg
    23701 tcgtgacttt atctgattat gtagatgaat ccatcgagct gaagggcctg ttgcttgctc
    23761 acggagatag tacggctagc agcattcaag cagtagtgaa tcaattatgc ggcaattatg
    23821 tttttgaagc gttggatatg ccagtagaga gtaatgtgtc agagattgtc gaatcgacaa
    23881 ggaatttcgt gaaacggcaa gtgaatacag atagtctagt gctgatcgta gatatgggct
    23941 ctttaaatca aatttacaca cagatcaaga acgatttaaa aggggagtta cttcttctga
    24001 ataatttgac gacttccatt gcacttgata ttggcttgaa gatgacgatg aatgcgccat
    24061 ttaaacagat tgcggaggat gcagcagaca agtattcaat caatgttcaa tactttgaag
    24121 gcttttctca aagtagcaac attatcattt cctgtatgtc gggtttgggg atttcggaca
    24181 aattgaaaga tgtttttcgt catcatttat cggatgaatc catcgaagtt ttcacgaagg
    24241 actacagaga actacgtgaa ttaatcgacc aaaatgatga aacttatttt gacaagacaa
    24301 aattggtcat cacgacttcg gatcttccac gttccttttc aattcctaat attaatgttt
    24361 atgacatctt agacggtgat ggagcagcca atttggagca tattttgagt cctgaaatga
    24421 agccggcaga atttaaccgc ttgattcagg agctagtaaa attcttttcg ttggaaggaa
    24481 ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat gagtgaagtg gaaacagtca
    24541 tttctaaata tgaaaattac tatcatttca ctttaaatgg ccgtgtaaaa ctaaatctct
    24601 atatgcatac ggcattgatg atggaacgtt tattcttggc tcggacagaa aaatctgaag
    24661 aaccgaatta tgatttgaaa ccagatgaag atgaatttta ttatgtttct agaagtattt
    24721 ttcaatcaat ggagatgaaa tataatttta aagtcaatgc ttatgagctt tcgctgctct
    24781 atgaattatt aggaccgtat attcaaaaat aaagtgtcta aaaaagtgtc taatctgttt
    24841 tttttaggtt agacactttt tttaatgtga attaacgtga ttaaaccaat gtttttaaaa
    24901 gttggcacag gaattgctta tataaaagtg aacagaaaga aaggaattaa ccatatgaag
    24961 aaaattctaa tagcgactca cggtcactta gcttcaggct ttcttagcag tattcaatta
    25021 ctgacgggga aggttcaaga gattacggtg attaatgctt atgtggatga gtgtgatttt
    25081 gaacaggagt tgaatgtatt tgtaaaccag gtgaacgagc aggatcagat ttttgttttt
    25141 accgatttat ttggcggaag tgtcaatcag aagattacca aaaccttttt ggagaaagag
    25201 attgctgtaa ctattttgtc tggctttaat ctgccaattc tgctcgaaat agtattggca
    25261 accaaggaat taacaaagga ggatattcag gaactggtaa ataaatgcca acaggaatta
    25321 aagatgacca ttcttgatga ggtacatgta gtaaatgaag aggaagattt tttttaggag
    25381 gaagaaaaat gattaaagca gtgcgaattg atgaacgatt aattcatgga caagtagcga
    25441 tgacttggac aaagagtcta aatttaaccg ggttagtagt agcaagcgat gaggcggcta
    25501 ccaacgatat tcaaaagatg acattgaaga tggctgcacc atcgaatgtc aaagtcatta
    25561 ttaaaacagt tgatgacgcg attcatttgt taaaagatcc gcgggcgaag gacatgcgct
    25621 tgatggtttt agtccctaat gtcaaagatg cggtaaaagt tgcgaaagct ttcccaacgg
    25681 aaatcgaatt aatgaacgtg gggaatgctg ggaaaatgac accgccatcg gaagataaga
    25741 aaaccttatc aaaggaagtg atgttaacga cgtcagaaat tcaagactta aaagaattag
    25801 ttgaacttta tccagatacc gtttttcagc ctacaccggc gatggaaaaa cgttcagcta
    25861 tctctatttt gagtgatttt tagaaaaagg aaggagaaaa attatgtatg atgcatttgt
    25921 agtcgctttg gccgttttta ttggagttgc tggacatgaa atgttcggga tgtccatgct
    25981 gagtcgtccg attgttgttg ctccgttagc aggagcgttg atgggggatt tgcagacggg
    26041 gttgattgtc ggggcgtcac tagaagccat ctttatgggt gtcgtcaata tcggaatctc
    26101 tagtacggcg gagccttctt tggctgctgg gctggctaca gcgttcgcaa tcaaaatggg
    26161 aggaaacgtg gatgcgatta ttcccattgt ctttccacta gcagtattag gattacagtt
    26221 aatgaatatg attttttctt ttgtatgtgg accgatggct gctaaatttt tcaaatttgc
    26281 aaaatctggt gatcaaaaag gaatcatcaa tctgcacttc tttatgtggt ttgtccacta
    26341 tgcgctttat gctcttattc cattctttgc cgttttattt ggagcagatg ttgtcaaaag
    26401 cgtcttggat aatattccag aggtcatcat gaacggatta acagtcgctg gaaatctgct
    26461 gccggcagtt gggatggcga tgctgttacg gatgctttgg agtaaaaata ttgctgtgtg
    26521 gtttttcttt ggggccgttg ttatggcgta tctcgagtta ccattgattg caattgccgc
    26581 agttggaacg attattgcaa ttgttatagc ccaaagagat ctacaactaa aaaatatatc
    26641 tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg cttgaccaag aagaggagga
    26701 tttctttcaa tgaaattagc tgatggaatt tctaaagatg agaaaaaatt ggttcggaag
    26761 atgttttggc gctcgatgac tatgtatgct tcggtgaatc cggtgacgat gggcggcggt
    26821 ggtttcgcct attctatgat gcccttcatt aatcatttct acaaaagtga agaagatcga
    26881 gctgcggcgc tggaacgtca tacacagtat ttttcgacga ccattccttg cgcgtccttt
    26941 gtcatgggga ttgcggcatc gatggagaag gaaaattctg aaaaagctgg ttttgacgtg
    27001 gaaagtatca acgcgatcaa aacgagtttg atgggaccat tggctggaat tggcgattca
    27061 ttgttctggg gagtttggcg tgtcgttacc gcgggactag cgatcaactt agccaatgcc
    27121 ggaaatattt tggcaccaat tatcttttta gttatgttca atatacctaa ttacttgatg
    27181 cgatactatg gcggcgtttt aggctattca ttaggctcta aatatattga aaagctgtat
    27241 gcgaacgggt taattgaaat tctgacaaaa gctgctagta tcttgggtct gatcatggta
    27301 ggtgcaatga cgatcagtat ggtcaaattc aataccgttt tgagcttttc aatgaagggc
    27361 gaagaactaa tgaatgtcca agaaatcctc gatagtattt ttaaaggaat tgtaccgtta
    27421 ttggtaacat tgagctgttt caaattacta gataaaaaag tcggcgtcat caaaattatt
    27481 tttggaatgg tcgtcttggg aattttattg tcattacttg gaatcgcata gtaggaagct
    27541 gcagctacta ggctgcagtt tttttagcaa ggagggaaaa gatgactatt aatgaagtaa
    27601 tttattcacc caaacaccca ttttttaatg ctgagttaaa cgacggaaat gtgactactt
    27661 caaacgaata cagctactat cggaatattc ctgatgtaaa gggattgacc ttcgatgtaa
    27721 gtaatcaaaa gacattagca aaccttgatg tttgcgggac gattcaaacg ttgacctttt
    27781 atcgagaaaa tttactaact gaggaaaaac caggtgtttg ggtcaataaa cagctctctc
    27841 aaaccaattc gttatcctta aaggttgaag taaacgggat tgtctaccat ttgtcagaat
    27901 cggatcatcg tattgaagtc gatgtgattc aagactgttt gcctagatat attcatcatt
    27961 attcgacgtt taaggtcatt gtagtatcct ttgctcccat actggctaat aagcgtttaa
    28021 gtatgcttgt tcaacaggtt tacattgtga atgaaacaca cgaaagcctt caagtttcag
    28081 tgaaagatgt gccttcgtat caagagaagt attcggatca gcaaaatgtg ttgatttctc
    28141 aaaaaggaaa taaggacacg atcgcacaac aggaagtggc aagcttcgcc attgcttttg
    28201 tggacccaaa tgcttttgaa gaagtaatga cttttcagga atctgatatc gaaaaatggc
    28261 tgaaggaaac attgaactat tttgctcagc tatttgggca gctgtcaatg ccaaacgata
    28321 gagttgtcca tctctatcgt cgtgcacttt atcaaagctt ttctagtttc ggtatgaatc
    28381 gacagggaca ggtcgtcggc agcaattggg gcagctatcc tgctactaat cgaatttgga
    28441 ataaggatat gtattacgca tcattgccat tcaccatgtt tgaaccggag ttatgtcaaa
    28501 aaacggttct atggtttgat caatatggcg ttaagtttcc tggttcaaaa tttacaggag
    28561 gtatcaatca ttctctgagt aattcgttat ccagtgcgtt actggctagt ctttattttg
    28621 aacatactgg ggatacaaaa ttctttgagc ggtgtccgca ggttctactg aatgcttcaa
    28681 gaattattga ggacatatta gctcaacgtc ggtcagggga accaatgctt tttccatctg
    28741 tttggttatc agacgccttt gcacttggaa agtatcatac cggcagcaat ctttgtcttt
    28801 ggaaggcctg tattggactt gcagaaattt ttgaagccct agaacaactg agtcttagca
    28861 agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg
    28921 atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa acaacctatt
    28981 cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
    29041 ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac actacgttga
    29101 tgccttttta tcaattttta gataataaag atcatttata tcagaacacc atggcctttt
    29161 ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact tggggattag
    29221 aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg cacaaccctc
    29281 aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac ggttcgtggt
    29341 ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac tttggctgcg
    29401 gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa tatttcggat
    29461 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
    29521 agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
    29581 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
    29641 aaaagggaaa aacgattcac attcaaagga gaaaaagatg agtatactaa aaaatattac
    29701 ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag gcgagaaagg
    29761 taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc
    29821 gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa taattcgtca
    29881 tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac gtgatttaat
    29941 cttaagaatt tattgggatg ggtcggatag ccctgctgtt gaaagtcctt taggggattt
    30001 cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgccgatag tggttaatcc
    30061 tacagggggc tttaattcct atttcgaaat gcctttccaa acatccgcta aaattacgat
    30121 cgagaattta catccgaagg atatgtcctc atttttttat acgataaatt acgctttggt
    30181 ggacgaattg gaagaaaata gcctatattt tcacgcaaat tggcatcggg aacgcattac
    30241 caaattacag gaggattata agttaattga tcgattaaag ggtcaaggct attacgtggg
    30301 tacttacctt gcgcttacct gcctagaacg ttattggtgg ggcgaaggcg agtttaaatt
    30361 ctacattgat ggggatagtg agtatccgac cgtaacctca acaggttcag aagattattt
    30421 tggcggggcg tgggcctttc atgagtacga tgagttcgga cgtccacatg cgaaaaacta
    30481 ttcaacagcc tttttagggt atccttacca atcaaataga gatgccacaa gagatcgttt
    30541 tgaaacggga aaattgaatg ccgttcatgc ctttggtgac gacgcaatgc cacgacatgg
    30601 tctttatcgt tggcatcttc aagaccccat ctgttttacc gaagatttat cggtcacttt
    30661 acaacaaatc ggcaacgatg atgttcgctt atttgagcgg caagacgatg ttgctagtgt
    30721 cgcttactgg tattcaacag aagcttcagg aaatactaaa cgtcatttca ctgatcgaga
    30781 acgcttgccg cgttaatgaa gtgtttttca catattcgaa gggaggaacc tatgatgata
    30841 tatggggcaa ttgaagctgg agggactaaa tttgtctgtg cagtcggtac tgaggaatta
    30901 acgattttgg aaagagtcag ttttcccaca acaactccag ttgaaacaat gaagcaggtc
    30961 attgattttt ttaaaccttt taaggaacaa ttggcaggca tcggggtcgg ctgctttggt
    31021 ccaattgata ttcaatgcaa atcaacgagt ttcggtcata tcacatcaac accaaaactt
    31081 gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat atttcaatat tccgattgaa
    31141 tggacaacag atgtcaatgc ggcttgctat ggtgaatatg ttgctggttc tggcaaaggg
    31201 ttagcaggtg ttgcatactt cactgtggga acaggtatcg gaggtggtgc cttgttcaat
    31261 gggactttcg tggaagggtt cagtcatccg gaaatggggc atatgttgat caagcgacat
    31321 ccaaaggaca atttttcagg aaactgtccc ttccaccaag attgtttaga ggggatggca
    31381 gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc agaatttatt ggctgacgat
    31441 tcgttttggc agattgaagc cttttacctt gcccaatgtg catacaatac aacactgatg
    31501 ttttcgccag atagaattat ttttggcggt ggtgtaatga aacaagaaca catgaagaaa
    31561 aaagttcagg ataaatttgt tgagcttata aatggatatg ttgaaatacc accaattgat
    31621 agctacatta ttacgcccga attaggtgat aatgccggaa taatcggcgg attagcattg
    31681 gccagaaaag cggtaagaaa taagcaacca taattaatga atgtttcatt gatactcatg
    31741 tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag ttggataaag cgaaaatgaa
    31801 ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca ttaaatcact gaaaggaaat
    31861 gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat accttgagga tattcactgg
    31921 tggcgtacac ttaaatagta atatattgtg ttactttatg tctgtggtaa tagttatagt
    31981 tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc ataatccttg gcttaatgtt
    32041 tgaatttttt tggtgataga ttttttcagc cgtataaaat aaatcgtaat tctattatag
    32101 agagttgtga ttataaaaag atagcaagaa aaaaaaagaa agtttctata cattttaaat
    32161 attttattcc tatttctatt gctaaaaaag tagaaattct atatttacct ttaactctcg
    32221 cttctcttgc aaatgtagtt agcattttaa tcgataataa actcaggtag ttatattacc
    32281 ttgaaatgat tgataatttt gtcacttcta ttttttttgc aataaatttt ttattttagt
    32341 gaaaaataag aaactatttc tttgaaggcg ataagttaga aaaatattat tg
//
LOCUS       DAFELO010000027        32242 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFELO010000027
VERSION     DAFELO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.027"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            298..1959
                     /locus_tag="LP452_001362"
     CDS             298..1959
                     /locus_tag="LP452_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2014097.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2029..2376)
                     /locus_tag="LP452_001363"
     CDS             complement(2029..2376)
                     /locus_tag="LP452_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL2014098.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2460..3401
                     /locus_tag="LP452_001364"
     CDS             2460..3401
                     /locus_tag="LP452_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014099.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKTQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="LP452_001365"
     CDS             3402..4445
                     /locus_tag="LP452_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014100.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="LP452_001366"
     CDS             4451..5521
                     /locus_tag="LP452_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014101.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="LP452_001367"
     CDS             5511..6464
                     /locus_tag="LP452_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2014102.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="LP452_001368"
     CDS             6476..7486
                     /locus_tag="LP452_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2014103.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="LP452_001369"
     CDS             complement(7771..8592)
                     /locus_tag="LP452_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2014104.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="LP452_001370"
     CDS             complement(8689..8967)
                     /locus_tag="LP452_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014105.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="LP452_001371"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="LP452_001371"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL2014106.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="LP452_001372"
     CDS             complement(11723..12310)
                     /locus_tag="LP452_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014107.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="LP452_001373"
     CDS             12485..13807
                     /locus_tag="LP452_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2014108.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="LP452_001374"
     CDS             13962..14738
                     /locus_tag="LP452_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL2014109.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="LP452_001375"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="LP452_001375"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL2014110.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="LP452_001376"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="LP452_001376"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL2014111.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="LP452_001377"
     CDS             16628..17257
                     /locus_tag="LP452_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014112.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="LP452_001378"
     CDS             complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="LP452_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL2014113.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="LP452_001379"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="LP452_001379"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL2014114.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19444..19980
                     /locus_tag="LP452_001380"
     CDS             19444..19980
                     /locus_tag="LP452_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL2014115.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="LP452_001381"
     CDS             complement(20020..21783)
                     /locus_tag="LP452_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014116.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="LP452_001382"
     CDS             complement(21786..23501)
                     /locus_tag="LP452_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014117.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24280..24504)
                     /locus_tag="LP452_001383"
     CDS             complement(24280..24504)
                     /locus_tag="LP452_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014118.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24695..27472
                     /locus_tag="LP452_001384"
     CDS             24695..27472
                     /locus_tag="LP452_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2014119.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27553..27972
                     /locus_tag="LP452_001385"
     CDS             27553..27972
                     /locus_tag="LP452_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL2014120.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27984..28454
                     /locus_tag="LP452_001386"
     CDS             27984..28454
                     /locus_tag="LP452_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2014121.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28471..29250
                     /locus_tag="LP452_001387"
     CDS             28471..29250
                     /locus_tag="LP452_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2014122.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29237..30058
                     /locus_tag="LP452_001388"
     CDS             29237..30058
                     /locus_tag="LP452_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2014123.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30075..31124
                     /locus_tag="LP452_001389"
     CDS             30075..31124
                     /locus_tag="LP452_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2014124.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31137..32141
                     /locus_tag="LP452_001390"
     CDS             31137..32141
                     /locus_tag="LP452_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2014125.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT        10501 a         5735 c         6576 g         9430 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaagtgt tgatttttta ataattattc attaaactct
      241 taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt acgtaaaatg
      301 aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc tggatgttat
      361 ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga tggaggaggc
      421 gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt tgcaacagat
      481 acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct tgatgaagac
      541 agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga cggattaact
      601 tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg
      661 gattacgttt atggctggca acgaacagta gacccagcta cagcttctga atatgcttac
      721 ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga taaatcagaa
      781 ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa acaaacacca
      841 tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc cgtagtggaa
      901 gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg accattcaca
      961 ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa aaatgatcat
     1021 tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt aaaagaatct
     1081 tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt gtctggtgag
     1141 ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc gcggacaagc
     1201 tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa
     1261 gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg tgacggatca
     1321 gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac gaacgaagac
     1381 tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa agaatattgg
     1441 gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat ggcttctgat
     1501 gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga aaacttggat
     1561 ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg ctcaacttcg
     1621 ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc aagcagcttt
     1681 actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa tgcagaatat
     1741 gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc ccgttgggat
     1801 gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa
     1861 aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc
     1921 gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc gtgagcatga
     1981 gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt atcctaacaa
     2041 agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga tcagccaaaa
     2101 gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt tctttcggtt
     2161 ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac
     2221 ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat cattagataa
     2281 ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca gaatcagtag
     2341 aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaacccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtatccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg
    23701 aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata tatcaagtaa
    23761 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
    23821 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
    23881 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
    23941 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
    24001 tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa aagaaaacaa
    24061 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
    24121 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
    24181 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
    24241 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
    24301 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
    24361 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
    24421 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
    24481 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
    24541 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
    24601 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
    24661 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
    24721 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
    24781 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
    24841 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
    24901 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
    24961 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
    25021 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
    25081 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
    25141 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
    25201 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
    25261 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
    25321 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
    25381 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
    25441 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
    25501 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
    25561 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
    25621 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
    25681 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
    25741 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
    25801 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
    25861 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
    25921 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
    25981 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
    26041 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
    26101 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
    26161 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
    26221 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
    26281 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
    26341 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
    26401 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
    26461 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
    26521 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
    26581 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
    26641 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
    26701 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
    26761 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
    26821 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
    26881 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
    26941 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
    27001 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
    27061 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
    27121 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
    27181 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
    27241 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
    27301 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
    27361 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
    27421 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
    27481 aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga
    27541 ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg
    27601 atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc
    27661 tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa
    27721 gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta
    27781 caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt
    27841 gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta
    27901 actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa
    27961 gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac
    28021 acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta
    28081 atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag
    28141 gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga
    28201 cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag
    28261 aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc
    28321 gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa
    28381 ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa
    28441 gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt
    28501 gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt
    28561 gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc
    28621 gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat
    28681 gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa
    28741 accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac
    28801 ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat
    28861 tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt
    28921 gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg
    28981 attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt
    29041 tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt
    29101 tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt
    29161 ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca
    29221 ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga
    29281 gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca
    29341 gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa
    29401 agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat
    29461 cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga
    29521 atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt
    29581 aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt
    29641 agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca
    29701 cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa
    29761 aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact
    29821 gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg
    29881 ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt
    29941 atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac
    30001 gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa
    30061 aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa
    30121 gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt
    30181 tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg
    30241 aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa
    30301 aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta
    30361 ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg
    30421 acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag
    30481 aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag
    30541 ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc
    30601 aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt
    30661 ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc
    30721 caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt
    30781 cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc
    30841 atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa
    30901 gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg
    30961 agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg
    31021 ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa
    31081 agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc
    31141 tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc
    31201 aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag
    31261 gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca
    31321 acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa
    31381 caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag
    31441 cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca
    31501 gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa
    31561 tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt
    31621 cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa
    31681 aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt
    31741 ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag
    31801 aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag
    31861 agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt
    31921 tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca
    31981 caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct
    32041 tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc
    32101 cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga
    32161 atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa atattggcat
    32221 caattacgtt ataaaacaca tt
//
LOCUS       DAFELO010000028        31659 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFELO010000028
VERSION     DAFELO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.028"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            271..1140
                     /locus_tag="LP452_001391"
     CDS             271..1140
                     /locus_tag="LP452_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL2014126.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1349..2374
                     /locus_tag="LP452_001392"
     CDS             1349..2374
                     /locus_tag="LP452_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL2014127.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2413..3363
                     /gene="manA"
                     /locus_tag="LP452_001393"
     CDS             2413..3363
                     /gene="manA"
                     /locus_tag="LP452_001393"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL2014128.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3457..5160
                     /locus_tag="LP452_001394"
     CDS             3457..5160
                     /locus_tag="LP452_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2014129.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5162..7195
                     /locus_tag="LP452_001395"
     CDS             5162..7195
                     /locus_tag="LP452_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2014130.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSYIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7206..7646
                     /locus_tag="LP452_001396"
     CDS             7206..7646
                     /locus_tag="LP452_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2014131.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7846..8973)
                     /locus_tag="LP452_001397"
     CDS             complement(7846..8973)
                     /locus_tag="LP452_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014132.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8976..9653)
                     /locus_tag="LP452_001398"
     CDS             complement(8976..9653)
                     /locus_tag="LP452_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014133.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9917..11683
                     /locus_tag="LP452_001399"
     CDS             9917..11683
                     /locus_tag="LP452_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL2014134.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11698..12135
                     /locus_tag="LP452_001400"
     CDS             11698..12135
                     /locus_tag="LP452_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2014135.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12148..13299
                     /locus_tag="LP452_001401"
     CDS             12148..13299
                     /locus_tag="LP452_001401"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL2014136.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13361..14059)
                     /locus_tag="LP452_001402"
     CDS             complement(13361..14059)
                     /locus_tag="LP452_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL2014137.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14052..15404)
                     /locus_tag="LP452_001403"
     CDS             complement(14052..15404)
                     /locus_tag="LP452_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL2014138.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15627..17027)
                     /locus_tag="LP452_001404"
     CDS             complement(15627..17027)
                     /locus_tag="LP452_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL2014139.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17188..17925
                     /locus_tag="LP452_001405"
     CDS             17188..17925
                     /locus_tag="LP452_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL2014140.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18050..18529
                     /locus_tag="LP452_001406"
     CDS             18050..18529
                     /locus_tag="LP452_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL2014141.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18526..19293
                     /locus_tag="LP452_001407"
     CDS             18526..19293
                     /locus_tag="LP452_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014142.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19523..20092
                     /gene="lepB"
                     /locus_tag="LP452_001408"
     CDS             19523..20092
                     /gene="lepB"
                     /locus_tag="LP452_001408"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2014143.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20196..20798)
                     /locus_tag="LP452_001409"
     CDS             complement(20196..20798)
                     /locus_tag="LP452_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2014144.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20904..21373
                     /locus_tag="LP452_001410"
                     /pseudo
     CDS             20904..21373
                     /locus_tag="LP452_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21479..22063
                     /locus_tag="LP452_001411"
     CDS             21479..22063
                     /locus_tag="LP452_001411"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL2014145.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22066..23139
                     /gene="prfA"
                     /locus_tag="LP452_001412"
     CDS             22066..23139
                     /gene="prfA"
                     /locus_tag="LP452_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL2014146.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23132..23971
                     /gene="prmC"
                     /locus_tag="LP452_001413"
     CDS             23132..23971
                     /gene="prmC"
                     /locus_tag="LP452_001413"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL2014147.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23964..24143
                     /locus_tag="LP452_001414"
     CDS             23964..24143
                     /locus_tag="LP452_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014148.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24165..25190
                     /locus_tag="LP452_001415"
     CDS             24165..25190
                     /locus_tag="LP452_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL2014149.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25365..26609
                     /locus_tag="LP452_001416"
     CDS             25365..26609
                     /locus_tag="LP452_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL2014150.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26762..27709
                     /locus_tag="LP452_001417"
     CDS             26762..27709
                     /locus_tag="LP452_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2014151.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27843..28472
                     /gene="upp"
                     /locus_tag="LP452_001418"
     CDS             27843..28472
                     /gene="upp"
                     /locus_tag="LP452_001418"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL2014152.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28611..28853)
                     /locus_tag="LP452_001419"
     CDS             complement(28611..28853)
                     /locus_tag="LP452_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014153.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28974..29046
                     /locus_tag="LP452_001420"
     tRNA            28974..29046
                     /locus_tag="LP452_001420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29007..29009,aa:Thr,seq:cgt)
     gene            complement(29165..29803)
                     /locus_tag="LP452_001421"
     CDS             complement(29165..29803)
                     /locus_tag="LP452_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2014154.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29926..30558)
                     /locus_tag="LP452_001422"
     CDS             complement(29926..30558)
                     /locus_tag="LP452_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL2014155.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30909..31208
                     /locus_tag="LP452_001423"
     CDS             30909..31208
                     /locus_tag="LP452_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2014156.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10823 a         5493 c         6361 g         8982 t
ORIGIN      
        1 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
       61 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
      121 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      181 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      241 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      301 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      361 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      421 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      481 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      541 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      601 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      661 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      721 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      781 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      841 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      901 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      961 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
     1021 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1081 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1141 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1201 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1261 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1321 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1381 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1441 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1501 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1561 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1621 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1681 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1741 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1801 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1861 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1921 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1981 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     2041 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2101 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2161 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2221 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2281 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2341 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2401 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2461 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2521 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2581 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2641 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2701 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2761 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2821 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2881 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2941 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     3001 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3061 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3121 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3181 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3241 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3301 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3361 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3421 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3481 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcacct aaatgaaatg
     3541 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3601 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3661 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3721 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3781 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3841 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3901 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3961 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     4021 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4081 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4141 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4201 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4261 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4321 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4381 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4441 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4501 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4561 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4621 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4681 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4741 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4801 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4861 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4921 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4981 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     5041 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5101 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5161 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5221 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5281 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5341 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5401 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5461 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5521 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5581 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5641 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5701 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5761 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5821 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5881 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5941 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     6001 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6061 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6121 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6181 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6241 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6301 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6361 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6421 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6481 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6541 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6601 caagcagcta aaagaagctt tttcgtatat tgagaagaaa aaaaggcaaa aaattacgta
     6661 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6721 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6781 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6841 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6901 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6961 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     7021 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7081 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7141 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7201 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7261 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7321 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7381 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7441 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7501 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7561 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7621 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7681 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7741 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7801 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7861 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7921 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7981 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     8041 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8101 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8161 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8221 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8281 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8341 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8401 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8461 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8521 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8581 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8641 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8701 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8761 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8821 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8881 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8941 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     9001 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9061 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9121 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9181 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9241 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9301 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9361 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9421 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9481 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9541 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9601 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9661 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9721 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9781 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9841 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9901 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9961 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    10021 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10081 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10141 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10201 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10261 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10321 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10381 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10441 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10501 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10561 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10621 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10681 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10741 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10801 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10861 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10921 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10981 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    11041 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11101 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11161 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11221 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11281 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11341 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11401 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11461 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11521 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11581 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11641 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11701 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11761 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11821 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11881 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11941 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    12001 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12061 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12121 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12181 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12241 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12301 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12361 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12421 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12481 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12541 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12601 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12661 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12721 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12781 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12841 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12901 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12961 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    13021 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13081 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13141 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13201 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13261 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13321 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13381 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13441 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13501 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13561 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13621 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13681 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13741 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13801 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13861 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13921 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13981 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    14041 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14101 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14161 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14221 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14281 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14341 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14401 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14461 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14521 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14581 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14641 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14701 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14761 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14821 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14881 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14941 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    15001 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15061 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15121 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15181 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15241 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15301 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15361 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15421 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15481 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15541 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15601 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15661 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15721 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15781 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15841 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15901 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15961 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    16021 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16081 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16141 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16201 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16261 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16321 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16381 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16441 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16501 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16561 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16621 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16681 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16741 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16801 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16861 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16921 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16981 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    17041 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17101 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17161 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17221 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17281 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17341 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17401 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17461 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17521 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17581 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17641 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17701 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17761 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17821 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17881 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17941 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    18001 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18061 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18121 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18181 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18241 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18301 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18361 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18421 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18481 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18541 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18601 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18661 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18721 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18781 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18841 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18901 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18961 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    19021 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19081 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19141 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19201 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19261 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19321 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19381 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19441 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19501 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19561 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19621 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19681 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19741 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19801 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19861 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19921 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19981 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    20041 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20101 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20161 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20221 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20281 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20341 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20401 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20461 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20521 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20581 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20641 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20701 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20761 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20821 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20881 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20941 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    21001 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21061 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21121 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21181 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21241 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21301 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21361 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21421 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21481 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21541 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21601 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21661 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21721 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21781 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21841 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21901 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21961 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    22021 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22081 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22141 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22201 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22261 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22321 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22381 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22441 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22501 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22561 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22621 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22681 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22741 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22801 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22861 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22921 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22981 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    23041 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23101 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23161 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23221 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23281 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23341 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23401 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23461 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23521 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23581 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23641 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23701 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23761 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23821 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23881 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23941 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    24001 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24061 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24121 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24181 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24241 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24301 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24361 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24421 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24481 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24541 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24601 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24661 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24721 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24781 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24841 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24901 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24961 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    25021 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25081 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25141 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25201 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25261 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25321 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25381 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25441 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25501 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25561 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25621 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25681 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25741 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25801 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25861 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25921 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25981 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    26041 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26101 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26161 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26221 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26281 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26341 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26401 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26461 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26521 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26581 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26641 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26701 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26761 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26821 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26881 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26941 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    27001 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27061 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27121 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27181 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27241 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27301 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27361 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27421 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27481 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27541 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27601 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27661 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27721 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27781 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27841 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27901 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27961 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    28021 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28081 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28141 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28201 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28261 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28321 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28381 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28441 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28501 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28561 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28621 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28681 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28741 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28801 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28861 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28921 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28981 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    29041 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29101 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29161 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29221 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29281 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29341 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29401 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29461 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29521 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29581 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29641 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29701 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29761 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29821 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29881 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29941 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    30001 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30061 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30121 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30181 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30241 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30301 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30361 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30421 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30481 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30541 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30601 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30661 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30721 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30781 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30841 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30901 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30961 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    31021 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31081 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31141 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31201 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31261 aggctaggtt catttatttg aacagagtct gtattttttt attctaaata aagaaacaga
    31321 tgtttcttta tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat
    31381 aatgttaatt aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcatttttgt
    31441 aagttaaaaa ttggaaatat agattgaaaa aatgaaagca ataagtaaaa taaaatgaat
    31501 atatgcgaaa aaataattta cattaggaaa aaccttgtaa aaacagtttt ttggcatatt
    31561 tattttttat aaatatttgt aaaataacta tcaatttttt agttaaaaga ttggaaatga
    31621 gtgggggagg aattgaagaa aggatttttg agagatgat
//
LOCUS       DAFELO010000029        31482 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFELO010000029
VERSION     DAFELO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.029"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..106
                     /locus_tag="LP452_001424"
     CDS             <1..106
                     /locus_tag="LP452_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBL2014157.1"
                     /translation="DDNGDELSVEDTWSYLSDYIVSVEIIEFVADKEE"
     gene            220..459
                     /locus_tag="LP452_001425"
     CDS             220..459
                     /locus_tag="LP452_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014158.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPTRPVIKRKKKITQD"
     gene            526..765
                     /locus_tag="LP452_001426"
     CDS             526..765
                     /locus_tag="LP452_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014159.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            892..1581
                     /locus_tag="LP452_001427"
     CDS             892..1581
                     /locus_tag="LP452_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL2014160.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1587..2753
                     /locus_tag="LP452_001428"
     CDS             1587..2753
                     /locus_tag="LP452_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL2014161.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2835..3506
                     /locus_tag="LP452_001429"
     CDS             2835..3506
                     /locus_tag="LP452_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL2014162.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3559..5535
                     /locus_tag="LP452_001430"
     CDS             3559..5535
                     /locus_tag="LP452_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2014163.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5548..6300
                     /locus_tag="LP452_001431"
     CDS             5548..6300
                     /locus_tag="LP452_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2014164.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            6316..7077
                     /locus_tag="LP452_001432"
     CDS             6316..7077
                     /locus_tag="LP452_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014165.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7090..7350
                     /locus_tag="LP452_001433"
     CDS             7090..7350
                     /locus_tag="LP452_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014166.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            7347..9437
                     /locus_tag="LP452_001434"
     CDS             7347..9437
                     /locus_tag="LP452_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2014167.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(9473..11530)
                     /locus_tag="LP452_001435"
     CDS             complement(9473..11530)
                     /locus_tag="LP452_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL2014168.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11675..11866)
                     /locus_tag="LP452_001436"
     CDS             complement(11675..11866)
                     /locus_tag="LP452_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014169.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12054..12509
                     /locus_tag="LP452_001437"
     CDS             12054..12509
                     /locus_tag="LP452_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014170.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVSYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            12506..12748
                     /locus_tag="LP452_001438"
     CDS             12506..12748
                     /locus_tag="LP452_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL2014171.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12766..13068
                     /locus_tag="LP452_001439"
     CDS             12766..13068
                     /locus_tag="LP452_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014172.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13137..15191
                     /locus_tag="LP452_001440"
     CDS             13137..15191
                     /locus_tag="LP452_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014173.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            15191..17803
                     /locus_tag="LP452_001441"
     CDS             15191..17803
                     /locus_tag="LP452_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL2014174.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17800..20178
                     /locus_tag="LP452_001442"
     CDS             17800..20178
                     /locus_tag="LP452_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014175.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20239..20781
                     /locus_tag="LP452_001443"
     CDS             20239..20781
                     /locus_tag="LP452_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014176.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            20859..21191
                     /locus_tag="LP452_001444"
     CDS             20859..21191
                     /locus_tag="LP452_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014177.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21192..21785
                     /locus_tag="LP452_001445"
     CDS             21192..21785
                     /locus_tag="LP452_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014178.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21807..23831
                     /locus_tag="LP452_001446"
     CDS             21807..23831
                     /locus_tag="LP452_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL2014179.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23831..25030
                     /locus_tag="LP452_001447"
     CDS             23831..25030
                     /locus_tag="LP452_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2014180.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25046..25690
                     /locus_tag="LP452_001448"
     CDS             25046..25690
                     /locus_tag="LP452_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014181.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            25701..26507
                     /locus_tag="LP452_001449"
     CDS             25701..26507
                     /locus_tag="LP452_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014182.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26530..26759
                     /locus_tag="LP452_001450"
                     /pseudo
     CDS             26530..26759
                     /locus_tag="LP452_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26780..27853
                     /locus_tag="LP452_001451"
     CDS             26780..27853
                     /locus_tag="LP452_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBL2014183.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            27897..28166
                     /locus_tag="LP452_001452"
     CDS             27897..28166
                     /locus_tag="LP452_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014184.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            28442..28717
                     /locus_tag="LP452_001453"
     CDS             28442..28717
                     /locus_tag="LP452_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014185.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            28759..29181
                     /locus_tag="LP452_001454"
     CDS             28759..29181
                     /locus_tag="LP452_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014186.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            29201..30793
                     /locus_tag="LP452_001455"
     CDS             29201..30793
                     /locus_tag="LP452_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014187.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30814..31149
                     /locus_tag="LP452_001456"
     CDS             30814..31149
                     /locus_tag="LP452_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014188.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            31295..>31482
                     /locus_tag="LP452_001457"
     CDS             31295..>31482
                     /locus_tag="LP452_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014189.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11333 a         4878 c         6413 g         8858 t
ORIGIN      
        1 ggatgataat ggagacgaat tatctgttga agatacttgg tcatatttga gtgattatat
       61 tgtcagcgtt gaaatcattg aatttgtagc ggataaagaa gaataaaaac tggaggaata
      121 aattttacag ataaaggctt agaaggagtc acgacgaata ataaaaaggt agtattacct
      181 taggcattga gtaaggaatt tatttaggag gaaaattaga tggcgcgacc agttttagag
      241 gcgatggata tggatacttt gacaaaagga aggtatgtac agctgttagc tcgtggttat
      301 tcacctaaag atgtattcaa atccctgtct aaaggaacag atatggaaaa agaaagatta
      361 cgaaaagaat tcgattattg gagaacccat aatggaatca aggatttgaa accaacacgg
      421 ccagtaatta aacgaaagaa aaagataacc caagactgaa gtgtagagcg tatgtgatca
      481 taggcttttt tatttttgca aaaaacaaga aaggtaggaa aactcatgaa ttatgaagaa
      541 atcgaaaaca gaaaaaaagt gtcgaaagaa atggaagaaa agttgctaaa aatgatgaaa
      601 caaaaacacc ttaaaagatt atctgtcatg caatatatca atgatatgaa gataacagga
      661 aaagagaaag cttgtttgtt aggttcaatg aaaaactttg aacagttgag gcgaacttat
      721 gtaaaaacag gttccaattg ccaactatta ttagaagttt cttgacgatt atgatatgaa
      781 aatgatcaat tatgatgtgg cattgttatt gaataaaaag aatagtaaaa tttaacatgg
      841 tttatcttgg ttagatgtag gtaaattaat tcatatatag aaggtagata gatgaataaa
      901 aacaataaaa ctttatttaa tccttacgtg ggcgtaatca ttgtttttct tctttttagt
      961 tattttgctt atcaaggtta tcaaaaggac aaaatcgctg aaaaagtaga tggtgtaaca
     1021 aaaacaatcc atattcagga acaggctaaa acctatcaag ataatttaac cagtttaacg
     1081 gatatttcta agagacaact gattactaat caaaaacagg cattagaaaa ggatctcaaa
     1141 ggtgaaattg ctggaaaact tgttttttct tctacaggat ttattctccc tgtttttcaa
     1201 ggagcgaata gcgacacgct tagtttagga gtagcttcta cttattatct agatactgaa
     1261 atgggaaatg gaaattatat tcttgctggt tataacgtag atacaccaag cgttttatta
     1321 tccgatatga ctgatttatc tgaaggagaa gaaattgatc taatcgatgc tcattacaag
     1381 tatcagtatg aagtctccaa aagaggaaaa atttctgatt acagccaggt agttcaatct
     1441 aaggaaaaca attccttttt atccttgcca aaagcaggta aaaaaccact actcacactg
     1501 ttagcctttc aagcaaataa tatgaaaaag tgcgatgtga tccagtgtga attagtcaat
     1561 gtagaaaaaa tcaataaata agggaagtga gtcacattca agaacttgta gaaaaattga
     1621 tcaataaaat gagtgccatt tttctgttac tgttggtatc cattctatat ttaatatgga
     1681 cggtggggtt aaatcctcct acgatattat ttatttatga taatgctgca cagttagtga
     1741 cgtttttcgt aggctgtttg tttcttttga atctgtacaa ggtaaagaga aacgatctat
     1801 tcttcattgc agcaggcatt cttttttacc ttttcttttc tttttttaga agctaccgag
     1861 gagatacttt ctatggtgat ctgatcgttc cgacaattat tgctattatt ttgattgtaa
     1921 aatggacgaa atttactgta attgatcgta gcttgtatct tgtagtattc tatgtctttt
     1981 tactcgtaac ggtttatcga gtatttactg agataccagt accagaagga caaagtattt
     2041 ggaatccatc gaacaaatta tcagatattt ggatcaatac aaatacgata ggtagttctt
     2101 taatgacatt agcccttctt atcacaggat ttgctagttc atttggaaaa tggtacatgc
     2161 gattgctatc attaccagcg ttgattcttg ctggggcaac gatttgggtt tgccaatcga
     2221 aagcagcatt atatgcgctt gttatcttca ttatcttaga aattttacct aagaaggttt
     2281 ttcgaaatac acacttgtcc ttttggatgt atgcagcagt ttcggttcta gctttaccga
     2341 tttcttattt tgctgcaatg tcagacaaag ttcacttatt tacaggacga gaagaaattt
     2401 ggcgcaaatt ctatgaaaca ttaggcgaaa aaacaggaca aatatttgta gggatgaaac
     2461 ctttcttttt ccatagagga aatcagattc taggtaatca taatacgtat cacgcactcg
     2521 tcaatcacta tggattgatt ggttttgttc tatcgattat tcttttgctt agtttcttat
     2581 ggttgctcgt aagaaaacgt gagctgacaa atggacaact aacattcttg tgggcttttt
     2641 tagcgatcat gtgtcaatcg ttcatggaag aaacattaac gacaatcagt tgggtgccaa
     2701 ttgtattctt aatgttagca atggctacac accaatataa aaccaccaga taaatgaaat
     2761 agaatagtaa taagagaaaa cactttttcg ttgtgaaaaa gtgttttttg ttttgtctga
     2821 aaggaggcag atggatgaaa aaatggttat ttggtttttt aggagtcgct ttaatcgtcg
     2881 tttgcagtgt gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa
     2941 atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca cgcaaagagt tataaggagg
     3001 atgcaacgaa cgtcaataat gatatgtctt tggggcaaat gttgtccata caaaaagaag
     3061 caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt
     3121 tagcattgcc aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt
     3181 atttttatga agatgccgaa atgggtaaag gaaactatgt cctagcaggt cataacatgg
     3241 aaatgccagg tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact
     3301 tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt
     3361 actttaaatt aatcgatggc gttcctgaag agaactcttt cttgtcttta ccaaagaaag
     3421 gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat
     3481 acgtggtaca gggagagcta caataacaag aatttaatca acgcgagtcg ttgaaatatg
     3541 aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgattgtt
     3601 ggaatgttag gaatcattgc aggctgcttt caggggtatc gagcttatgc agaagatgtc
     3661 acgcaaaaaa cgccaccaga aagggtcaat atcacggttc ataaattaat gtacgatcaa
     3721 agtacgcaat tgaacgttga tattgacgga atcaaaaatg atggttatac gcacgatcaa
     3781 tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta
     3841 ggaaatatta cggatcaagt attaccgact gaagattctg atctaaccaa cgcgaaagtg
     3901 gacgaaatcg ttaaagatgt agaagacaag ggatctaaca gtgagtacgt taagaatgca
     3961 accaataaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttgc tgatcaacca
     4021 gcctatgtca attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga
     4081 ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga caacacagga
     4141 tcaggatttt taaaagatat tcatttgtat ccaaagaaca ttgtttctaa gttgagtttt
     4201 gaattaacga aatttggtga cgatggcaca gctcaaagca aacaaacacc tttaaaaggg
     4261 gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattagg agagcttgta
     4321 tcggatgatc aaggaaagct gactgcaact gatttgacgt taggaaaata ctactttgtg
     4381 gaagttcctt cagaagttgt agttggatca gatcaagaac caacagcaga ccaatatttg
     4441 ttaggcgcag acgctcgcaa cgatgcgcac aataagttaa cctttgaaat tacgaatgac
     4501 ggcgtgacaa gtgatttaaa agcttcctac gtgaactata aagcaccagt gatcgataaa
     4561 acagtgacaa atggaacagg agaagaacat tctttccaaa ttggtgacgc agtgaattat
     4621 caaggtacaa ttcatatccc tacagatatc gcaggtggaa cagatgggat tacagtaaat
     4681 ggtgtaaaaa gtgaaacttc tccttacagt gtatttaaat ggggcgacac agcaggtaaa
     4741 ggactttctt atgttgctga aaaagcagat attaaagtaa caaataatga tggttcagtc
     4801 gtattaaaag aaaatactga ctataaaatc gaaaattctg atagtggttt tgtgatcgat
     4861 tttatcgtaa ataatggaca agtaagtgat acagttgcga atttacatgg tcaagatcta
     4921 aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa tcgcaaatcc attaacaaat
     4981 agcgtggatt tcgtttataa caataatcca tttaatcagg aagaacacca cgaaaaaacg
     5041 aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag tcgatagcgg cctatttgga
     5101 acaggtatca aagcaacacc gcttgaaggc gcagaatttg cagctaagaa tgcagaaggt
     5161 aagtactacg gtggacttac tgatacagat aaagatggtg tcaaagaagc agtttgggta
     5221 gacgatgtag caaatgcagc aatcctaaaa tcagataaag agggtcattt tgaaatcact
     5281 ggtttaactg aaggagatta ctcactggaa gaaacaaaag ctcctgaaaa ctatcaaaaa
     5341 ttaacaaaag agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc
     5401 acgatcaaaa ataatcaaaa agcttccgta ccaatgactg gtagcaacgg tttccaatct
     5461 tacgtattga tctcttgctt gttgcttggg gcaggcgcaa tttctgcagt tgtttacttt
     5521 aagaagaaag cctagaagga tcagctaatg attatcagac accctaagaa aaagcgaatc
     5581 atgggtaaat ggatcattgc tttctggtta ctttcagctg ttggtgtctt acttttgatg
     5641 cctgctgaag cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga
     5701 acaggaacgg cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat
     5761 cctgttggta ttcttacaat tccaagtatt tctttaaaac taccaattta tgacggaact
     5821 tccgacaaaa ttttagagaa tggtgtaggg attacggaag gaactggcga tattacagga
     5881 ggaaacggta aaaatccttt aatcgcaggg cacagtggac tttataaaga taatcttttc
     5941 gatgatttgc cttcagttaa aaagggagaa aagttttata tcaaagttga tggtgagcag
     6001 cacgcttatc aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt
     6061 gtcacttact tagaaccaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct
     6121 aaggggatca acacacaccg tttcctagtg tatgggaagc gagtaacctt tacaaaatca
     6181 gaattaaaag atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt
     6241 actgtttttc tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa
     6301 aggagggtaa ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt
     6361 tctttcactg cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca
     6421 gaacaaacaa tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat
     6481 tcgtcaacag ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa
     6541 cagaccattc acgttcaaaa aattatttct tccgaacaat tagacaacca ggggcaaaag
     6601 cttgaaaaac aagaaggagt aaatggcgcg aagttttctg tttatgatgt atcggacatt
     6661 ttacaaaaga tggatgttaa ggatctcact actgaccaaa ttgaatccca attaaaagat
     6721 cgagtgaaaa aactatccag tgatcaactg aagctaattt ctaatggaga aacaaagaca
     6781 attgatcaac aaacaggtgt ctttgaattc tcggtagaag tacaggcaaa tcagaaacaa
     6841 gcttattata ttgtcaatga atcaagtcct gaaaacattt ctaatagcga ggatattttg
     6901 ctattaacac ctgtttctga taaaaatggt gaattcttaa aggatgtgtg gatttatcct
     6961 aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa tcgtatctac tggcgtaaag
     7021 aaaaacttct ttgagaattg ttgggatttt gttactcact tgttttctag agattaagga
     7081 agaaataaaa tgaaactgat caaattatta ttattcaatt tttcttgttt aaccgcagtc
     7141 tttttgtact gcggtttatt attttctaaa ctatctattt tgccaagcta ccaactatac
     7201 ctaatgagga ttttaaacat accagctgct tccatgccgc tagatattat ggaaaagctc
     7261 attaggaatc cattatttac tacgatcaat ttgatcgtta ttgtcggaat ttcactagtc
     7321 atttataaaa aagaggggat gaagggatga attttaaaca ctcgtggaca agacggttaa
     7381 aatatgtttt tcccataatg atgatgttag gtacattgtt tgggttaaaa acagccaatg
     7441 tctttgctga aaaagttatt gtcgatccgt caaatccaat ccagaacgta aagaataatt
     7501 ttattattcc aaaagatgtt cctaatgcaa aaacaaatat tactgtaaac ggagcgtcaa
     7561 aagtttacaa atatcatgtt gatgccaaca aaggtggttt tacagacgac tacgtaggat
     7621 tagtggaggg aaacaaaaat atacgctatc cttctttcca aaaagaatac ggtgaaacaa
     7681 accttgtttt agaaggcgta acaaccaaca ccaaagtcaa tattgactat ggaaaaatcg
     7741 gtacgtataa cgggaaaaaa gtcaatatca agctagttct atctaatatt cacttatact
     7801 cggatacgtt gccgtggaat ctactagata acaactatac aaaaactcat tttcgagacg
     7861 atggctataa aaatacgaat ggtgcaatga gtaaatctaa aaaaagaaca gtactttgga
     7921 tctcagacaa tcttttctca ggaattgttt accattcaac acaaatgaat gtgcagctag
     7981 tagccacata tgaggacggt agccccgttc aattttcagg agatacattt atcagtttta
     8041 actctttgaa ccctgcagga ggaaaaagca cggatcttaa aggagaatat gcccactatg
     8101 acaaaatgaa tacgacagat tggtatgtca gaaaagatac ggttctttca gaatttaaat
     8161 ctttctacaa caatctgaac gtggtaggtg gccacccagg aggaagctct aagctgacac
     8221 aagcggataa tgatttcaat aatttgcatg ataaattagg agatccgaag tttggtcaag
     8281 gtactgtgtc ctttaaaata tctgaagcaa atccgacgtt tgtgattggt tcttctaatg
     8341 ttcaaacctg gtttacttta tcttctgcga caatctttag tgttgttcct gatcaacctg
     8401 aaaaaacagg ggttgataaa aatggaaata acgtgaacga taaaatgttg caagtaggag
     8461 acacgattca atatcgaatc aaacagaagg tgaatcgatt aggagtggat ttactagcgg
     8521 tttacgatcg atttgagtta atcgataatt taccaaaaga agtaaattac gtcgatgcac
     8581 acgttgaaag tggtacaaat aaaaaattcg atgtatctgg tgaagtgacc tatgacaaaa
     8641 caaaacacca ggtaaaatat gcagcgaaag ctgatacatt aaaaaatcga atgaagtata
     8701 acggcgaaac ttacgaatta gtgatcaatg taaaagtcaa tgaattggcc aatcaaaata
     8761 gtgtagccaa aaatcaggga acgtcgatca ttaataaagt agaaaaggat acaaatatca
     8821 ttaccgttta tttccctaaa attccagtga aagaagtaca acaaaatgga aaagatgtaa
     8881 acggacgaaa tgacggtaag aagggtacgc cgacagctcc tttaaacgca ggatctgaag
     8941 tacaatatct agtcacacaa aaatggcaca caaaaggcgt agacgctgcg tcagatcact
     9001 acaaacaatt tagtattcaa gatccaattg aagctcgttt gacatataaa gaaggttctg
     9061 cgcaagtaat agataagagt aaaggaaaag atattacttc tgaaggcaca ctaacctatg
     9121 atagcaattc aagaacgctg aagtgggaag cttccgctga cttcttaaat aacaatcttt
     9181 tagatggacg agaaattcag ctgatattta cagctaaaac tccattacaa tcagaaaaaa
     9241 atattgataa ccaagccgta gtagcagtag ataatgtttc taataaaaca aatgttgtga
     9301 caattggggt tgatcctaac ttgccacaag ttattgttcc taaaacaggt tctacacatt
     9361 tagtaacgat tttagcggtc agcttactat tacttgtgtt agcaactttt ggctacgcag
     9421 ttctgaagtt caattaaaca aaaaaacaag gctgaaaatc agccttgttt ttctatttga
     9481 aaatgggatt ggcactaata aatacgttgt ctgaagaaag tgatccgtct gcagaaactt
     9541 cataagcatg ggataaacca acgatacttt gattgttgta aagcaagttt gcgttatgag
     9601 cagcgctatc tttccacata accatagcat ttactgctcc tgtatctgaa aggatctcgt
     9661 caacgtaaag gtagaaacta ttgatatttt ctcctttagc accgtagcag cctaagcttt
     9721 ctaagagatc ttggaaagta ccgccgccag gtcggtaatg actgaagctt acttgtaatt
     9781 cttttgcacg aatatctgca gcttgttgaa cgatatcatt gaattgcatt ggggcaacgc
     9841 cattttgttg acggtagcta tttaaccaag caagttcttt ttgacgcaca tagttgttta
     9901 aatcacttag tgagatattt ttgtatttga aagtgatcgt tcgattttgg tcaacattat
     9961 tgattacacg ttgtccattt ttatcaacgg taacagatga atcgtcattt ctacacaaga
    10021 agtagttttg atcggtcata tttttccaag aagtagagaa actatctccc tcgtaaaccg
    10081 tttgtgtttc agtagataaa acagcgtttg tgtcaacgtt tatatggttc acagtgacgt
    10141 tgtattgatg agtataggca aaattgattc cttgattact tgtaacgttg tttaaagtca
    10201 cagtgttgct agagctttct ctaggatcaa gaacaaaatt agtgaacata ggtgcttcaa
    10261 ttgtgatgct ttgtccataa acgacgggat aactcttact gtaattatag agttcttgga
    10321 tatttggatc agtatccgag actttatttg gacgagcttt cacttcaact agaaagactt
    10381 catcatagta aaaagtaaat gtggtatttg tgttaatcgt ttgtgtttgt gtttcattcc
    10441 catttacttg atactgtggt gcttcaaagt tagtcaagtc ggttctagct tttgcagtgt
    10501 aacttgtacc agcttttact gagacatgtt cttcttttag cagcgttcca gtttgtcgat
    10561 tcatatgacg gacagttacc gcatattctg aaggtttagg tttgtgttgt aaccctttaa
    10621 atgcagaatc aagactattt ttagcattat ttacttgggt ttgagaagct ttagaatcgt
    10681 ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat ccttctgtaa
    10741 aatctccttt agcagttgat tttacttggt tgtacaaggc ttgtaattcc gttttgttca
    10801 cagtaggagc tggttttttc tttaatccct tgtaagcaga atctaatgtg tttttggcac
    10861 tatccacttg tttttgagtc gcttttgagt catttaacac ttttttagca ttgtttaaag
    10921 cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttagtt cccttaacgc
    10981 tcgtataaag agcttctaaa gctgatttat tcactttggg tttagaggtg tcagcaccat
    11041 accaaccaat accttcgtat ttccagccat agtctttggt taaccattgt acttctttcc
    11101 aacttgcagt gtagtgatgc gaaccagagg taacacctgg aaggaaaagg cggtagatcg
    11161 gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccaa ccgtaatttt
    11221 tggtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga aggcctttat
    11281 tgtataaacg atagacaagg tggccaccgc tagtcggtgc ttcccaagcg gggccttcat
    11341 aattacccca cttaattttc actaggtgat cacgttcttt tgtgctagag gtgtaaaaat
    11401 gttcatggtt atttggatta taaaggcgat acatgacgtt agttgaagca gcgtcagcag
    11461 atccagcaag agcaactgtg gcagcaaatg ttccaatagc tataacagtt tttgggttta
    11521 ataatttcat gttttcaact ccctattaaa tgattaaata ttttgtttac attcataata
    11581 taccacggta taccgaggta gtcaacggta taccgtggaa attttgaaat aaaaaagccg
    11641 ccctatgaag agtgtaagga tggctgttta tatgctatga agaaatggat tcggcgtatt
    11701 tggttagttt ggcagcatta tcaaatcgca ttttttcaat cgcacttttt ttctttatta
    11761 aatccgcaat tgttgattct gcgactgaag aatgtttgct aatttcatat gctgtcttgc
    11821 cagtttcaaa tagtaaccat tcgattttct ctttatcagc gtacattgca atgctccctc
    11881 ttgatttttt ctttattata gctctttttt gtaataaatc acaatatcaa gaggaaaagt
    11941 ttctatagaa aggtgggaaa ttatgagtat ccgttccccg tgtatcaaaa tgaggtttgt
    12001 tgctactttc gttatttaat atactaaaat aaaacaacga aagaaggaat agaatgaaaa
    12061 agttaataat ggtatttact tcgttgattg ccatgattgt tattttaggt ggatgtggta
    12121 gtgaaaaaaa taagcaagat agtcaagata atggcgtgtt aaaaccagga acagtgtgga
    12181 aagaagaagt aggcggactt gttcataatt taaaaattat agatgataca acatgggaat
    12241 attccgaaag tgtttggcat cctgaacaag tacaaataac agttgagaag ctaaaagact
    12301 ataaaggatt agaaagatat aaggtaatcg attcaggtgg agtagattta ttcataggta
    12361 aaacagcctt aatagtttcc tataacaaag acggtttaga aacgtttaaa tttattccaa
    12421 caaacgaaaa agaacatcga gataaaagtg aaatattaaa agaatacagt actaaatctg
    12481 gatacaagtt acaaaagact agcgagtgat tgctagtctt tttatttatc ctgaaggagg
    12541 aatcgcagtg gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg
    12601 tcgcggtgta tctgaaacac agtggaaaca agaagtcctt gaaaaagcac aatttgacct
    12661 attacgggga aaagacaagg acttagaagg atatatctat caacgtgaac gtgaaaagct
    12721 tgttttcgat gaaattatta aacactaaaa ggagcgaaca attttatgtt tgaatcaatt
    12781 acaatgaatt ttttaacaca accgaaagta gtagctgcgg ttgatatttt tagagcatta
    12841 caaggtgcag caactagttt aaaaaacaat ctgatgattt ttgcggctgt agctgccgtt
    12901 gcctccattg ttcttggggg gattttcttc ttctttggcc gaagaggagc tgaaaacggg
    12961 aagatgcacg taggttatgt attgatgggg gtaggagtta tattagctgc cgcctctatt
    13021 gtggtttcgt tatttggaat gtttggacaa accccacagg gactataaaa aataaataga
    13081 aggagtaaaa agtacgtggc tactttttac tcctttttta tgaaaggaga atactcatgt
    13141 actttacgat ttcaggcaat accaaaaaag ataaagagtt aataaaaaaa gcaacgaaga
    13201 aagtgggaaa gcgtactgcc tttttaccga aaacaattaa atattctgtc attgaaacgg
    13261 aagaacagct acatgtgtta ggacaagtgt tagaacaaga gaatcaatct tctgttaaat
    13321 tgactctcac gttacatgat aaagaaggaa aaatagaaaa ggctggcaca gaatacgttt
    13381 ttggcccact ggattatcaa atttcagaag gtttttatcc tacattgatg gatttgatcc
    13441 gccttgatgt gtttaaggaa acggatgaaa ccatgagtgt ggaagagtct tatcgaaacg
    13501 aaaaactgct acaagtttta gaagaagagt tagagagcga ccaaagaaaa gtgagtcaag
    13561 tgagtgaagt cgaagtaaat gatgaagtac catgggaacg accaatcttt ccaaagaagg
    13621 agtcaaaaga aaaagagaca gtctatgttg acgaacaagc tgatattccg actcagaaat
    13681 cgatatttga ggaagagaac gatgtaatgg gtcaattgga ggtagatccc tttgaagaaa
    13741 caatggagga tgaagagacg gccgaagtag aagttgatct agttgaagct aatgaagagc
    13801 caacatggac tgaaggggat tctcaggaat atattgaacc acttatcagt gaagaagtag
    13861 agaaaaacga aacagagata caaaaggcaa ctgtatttga ttatattttg gatcaatatc
    13921 cgccagagca tgaatggatg aaagaaaaaa ataaattatt tttaaatgag ctttattcga
    13981 aatatcaatt accccaaacc tatcaagcat ttttagagag taaagaagaa ttaatctcac
    14041 agggtaaagg cacactttta gaacagttag agtctgttca atcaacaaat tggcatgagc
    14101 ttgcacaaga agatttatta gccacttttg aagaacgtca acaagaaaca gatcaagaaa
    14161 taaatgagta tgcgacggaa cagtcatcta attgggagag aacaaaagag gaactaaata
    14221 aagaagaaca acgagtgatt gaagaagaag tacaaaaaat taaagagcga tacgaaaaga
    14281 aacgcgacaa tgcgtattcc ttgtgtatag agaaaattaa taactttact aaaactaatc
    14341 aagaagcttt atcgaaagaa aaagaaaact tgttaaaaga acgtataaaa gaacgaaaag
    14401 aagagttcta ccgaggttta cgtacagata agctaaagat tagcaaagaa ttaaatgaac
    14461 aactgaataa actttatcaa acaacgactg aagttcttat ggaaagacac gggcagatcc
    14521 agcaagaatt acaagcacaa attccaaaat ggaaaaaaga gtatgaaatt caacaacaag
    14581 aattgaaaga acaagaagaa aaacaacgac aagaagaaaa agagcgcgaa caattagaac
    14641 taaaaaaacg tgagcagcat cttgcagaac aaaaagtggc gatagaaaaa caacggttag
    14701 agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa atggatacat
    14761 tgcgacaagc acagttgaat tatctagcac aacctcaacc agtagctgct gttcaaacgc
    14821 cagtgaacaa ccaagcaaat ttaactcctt caccacagcg ttcctttaac ggatggatgg
    14881 tgggatgtat tgcaagttta agcttgcttc taggtggagg aacagtattt gcttttaatc
    14941 atatcaatca atcttcacag gcagattctg tagccacaac ggcgtcaatc aaggaagaag
    15001 cccaggaaca agcaagaaaa gagtatgaac aacaattgga agaattattg aatcgtacca
    15061 atcagtcaac agcagagtca agtatggaaa atagcaaaga gagtgatcct agtaaggcta
    15121 aagaaggatc aagcgattca actgtacact caaacacttc cgaaagttca gaatcaaatg
    15181 aggtgaaata atgcgtatta gagaaacaac gagacgaaaa gacaaacgat tcaatttaga
    15241 aaagaatagg tatctttctc atttctatcg ctatactcct tcaggagaag agctaccaga
    15301 aaaacatgac aaacgtattt ctcaaaagat tttttatagt ggcttagctt atcttccttt
    15361 aaactatgtt gttacaagaa taccagaagc atttcatcga tttgagatta ttccaggaag
    15421 tttacaagga gtgaatattt ttcagttaca agaaatgccg cagttattcc attttgattc
    15481 aactggtttt tggaatcctt tgaccccaac aacaagttta ggcacacgtt tagttgtttc
    15541 tacgatttgt gttgttccag ctgtacttat gggattgaaa atggacatgt ggtttcgtcc
    15601 gctagcagat gggcaaaaag gagataaccg attaaaaacg ttaaaggaaa tccaacaaat
    15661 ttatccgcaa ataccagaat caaagggaag ttttcctggt tatggcggaa taccaattac
    15721 tcattataaa aaatactggt atattcagac ggatacagta aatacttgta ttgttggtac
    15781 ttcccgctca gggaaaggcc agatagaagt tttagcaacc attgacaatt tgtcgcgtgc
    15841 agaaagacaa agttcaatgg ttgttaatga tccaaaaaca gaattatatg ttgctagtaa
    15901 agatgaacta gaagctagag ggtatgatgt gtatgccttt aatatcaccg atccactgca
    15961 atcaatgagt gataatccat tggcactcat tgtgaaatac tggaagcgag gagacataga
    16021 caccgcaaca cagttaacca atacttttac gaatacgatt tattatgatg caaatgcttc
    16081 agacaataaa tactttaacg aaaatgcgca gaaagctgtc aatgcgctta tttttgcgtt
    16141 attggaacaa gcggatcaaa caggggatta ctccaaatta acaccaaata atctcgttga
    16201 actactttca gaaattggcg gttttaatta ttatccagat cctgagaatg aattcaagca
    16261 gctcaatgcc ttagatgagt tcatgaatca gttaccccct ggaaatatag caaaaaaaca
    16321 atatggggca acaaaaattg gatctgataa agcgaaaggg aatatcttgt ctacggcaat
    16381 tacaggattg aatccattta ccctaacgaa aattgcgaag atgacttctc aatcgaccat
    16441 tgattacaag tccattggtt ttccgaagta tttggatctc aaattacatg aaagcctatt
    16501 aaatcaacgc attgtcgtgg aattttatca taagggaaag aaatttcacg aggaacgagt
    16561 caaagttggc tatcgtggtt tttgtgaaat caactttgat tgtcatttag agcagggaga
    16621 tcatgtttgc ttaaaataca atcatcaagg aaaacaatat gttgcttggt atgagttttc
    16681 tcaaaaagta ttgaaagacg aagagggaaa aatcgtttat cgaaaaaaac gaggagaaac
    16741 tcatttgcca gaactagaaa aagaagccgt tctaacatta gatgagaaga aaagtaacct
    16801 attagtagag tctattcgga tgcactatac cgataaacca atggctattt ttatgttaac
    16861 gcctgattat gatccatcta accatatcat tgttagcatc tttcttagtc agttgtataa
    16921 agaattatct acgcaatgtg ttaagactaa aggagataaa tgtcaccgta ggattcattt
    16981 tattttagat gaatttggaa atatgccacc gatggacaac atggaaggaa tcatgacggt
    17041 tacggcaggt cgtaatatgt tatgggatct gtttattcaa tcttatcagc aactttatgc
    17101 gaagtatgga gaacaaaacg cagcgattat caaagaaaat tgtcagacac atatttatat
    17161 tatgtcgaca gatgataata ccatcgaaga attttctaaa aaagtgggga ataagacggt
    17221 tgaacaagag aacagtacca tcaattcatt aggtatgaat actcatatca atcgcaatgt
    17281 tgattcagat cgtatcttaa ctcctgaaag aatttctact tttttagaag gtgaaacgat
    17341 tgtactcgct cctttaaaac gacaagattt gaaaggtcat aaaatacggc cgtatcctat
    17401 ttttaatcat ggggaaacga atcttcctta tgcttatcaa tatattgaag atttcaatcc
    17461 atcgtgggat atcaatgata tagacataca ttgtacacat gcggacttgg atcttaaggc
    17521 tttacaaatt gattatgatt atttcctacc agatgaatat gctagaaatc tctatcatgg
    17581 actgcatgat caattagaag aaaaaaacga agaaaatatg ttttataaaa tcgttcaatc
    17641 ttttgagaat gagacggcta aaaaattgtt aattcaattt tacgaagaac aagcaacgga
    17701 tcgtcttcaa gaatatgttg aaaagttgat caaaaatgaa gaaatttctg aaatgattag
    17761 gaaacggctg ttattggaag ctgaaaaggc ggtgaaagaa tgatcattgg aggaattttc
    17821 tctggcatag ccgactggtg gtcaggttta ttcggagaag gggatgcaga tcccaaaacc
    17881 gtaacgaaag tcttacaaga ttatggtaat tatctgcagt atcaagatgt attcagttgg
    17941 atttgtaata cttttttatg gggattgatc aaagggttat ataatctcaa tactttatta
    18001 gaaaaaacaa tttatcaaag ctttgattta aaagacttat tgaacgccgc tggtgttaat
    18061 gaactatttc aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt cacattaatt
    18121 tatttaggct tgaaattttt tatgagtaaa cacccaccga aagtcaaaaa tatcttggtt
    18181 aatttattta tggccatgat cttgattttt ggtggtagtt ctttaatcga tgaagggtta
    18241 aatatcagta aaaactttta tggagacgtc actgcagcaa ataaagcaga taaaacgggt
    18301 tcacctgctt ttcaaattat tcaaaataat gtgattgatg tcagtacatt actagcgact
    18361 gacccgaata aagtagaatc cttaccaaca gataaaagaa atgctttaac agcgaaaaat
    18421 ttcaaaacag cgaatatcaa tgggattatt acacctgatc aagctaagaa aatgtctgaa
    18481 tcaagtgata ttagtacagt acaaaaagat cggttaaagg cattacagta tcgactagag
    18541 ttagacgata atgggaaaga aatccctgtt caaattagtg atgaaggttt agcaaaatat
    18601 gtgtatacgt caggctatcg tcgatacatt agtagtccag ggattatttt ggctggggaa
    18661 tcttctttgt cggtagccta tttatttatt ttgtttacaa tcgttacttg cattattgag
    18721 ctagtattta aaaaattcta tttagtaatt gccgcgtcaa cggactttga gacaggacaa
    18781 cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc gtttacgatt
    18841 ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca tgcggtagga
    18901 aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc gctattcaaa
    18961 ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa cggcagcaat
    19021 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    19081 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taagaatgga
    19141 tttggccatc gaaaagatat tacccatgat aacggggaag aacaatcaga cgataaagct
    19201 acaactgtac ctaatagtaa gaagacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    19261 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagtt
    19321 gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg cacgagagct
    19381 aaagaatttg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    19441 gcttcagcag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    19501 gtgaaggcaa atacaacaag tgaaaaagaa aatcaagaag gtgcagcgat tccttcagcc
    19561 aaagatcacc aagatgagtt acattctaac gaaaattcga tcggagaaaa agattcagaa
    19621 gaaggacaac atggtcagtt accaaaaacc gatccagaac aatatcgtta taaatctaac
    19681 attaattctg aaaacgaaaa acgcatttca gatttaccaa aacgaaatca agaagtacaa
    19741 acggatgagg attccatcga tctgccaaat gataatggca tcgaagttgg aactgaaaaa
    19801 tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa tgaaagatag tcgtttgaat
    19861 caattcaaag agaatgatca aagctttgtt catcaatcac ctgcaggtac tgttcaaatg
    19921 aaatcaagtc gtttgcctga aaatggaaat ctgacaactg tttcttacaa tttagctgaa
    19981 agtggtgaaa cagagaatgc gtccgttcaa aatattgtga caaaagaagc tgcacctaag
    20041 ccaatcaata aagttcataa tggatacaga ccagcaaacg tagcatttac tgcaccgaca
    20101 aaaataaatg tgacaagaga aactaaaaac aggaacaatg aattgaaaga acaactattg
    20161 aaggatatca aaccataaaa ggcctattta agggcctttt tctttataaa acaagtttaa
    20221 aatggaaagg agaaaattgt ggaggatatt ttaatagcta cgtttataac tgcactggga
    20281 gcatttttaa cgatatttgt ggtgactatt aaaggattta aagatacgga tagtttacaa
    20341 aaagatcatc agtctatttt agatggtcaa aaaacacata ccgaaagcat tattgtacac
    20401 ctttcagaag gattcactta tttgaaagat gctatgccca cattcacagt aaaattaacg
    20461 gaaatatttc attctgttga gcaaatatcg tcagaattga aagagcaaag acaacaaaaa
    20521 gatcgtcaac ttggctcact taattttagg caagaaacga tacaagaatc attggagaat
    20581 gtaaatcgat taaacgatga aatgcaacga gttcatttgg aaaatgaaga attacgcgca
    20641 caaaattatg atttaattca aaagaaaaga aatctggaac aaaaaattgt tgaattaaaa
    20701 gaagaaaatc gtcaattgaa ttatcaatta cgacgaggaa aaaatcgttc tcgtgattta
    20761 ggaaatgaat tagagttgta atatttagaa aaacgctgtg agaaatcaca gtgtttttta
    20821 tttctaaaaa tacaaaatat aaggggtatg gttatattat ggataaatac attcatatga
    20881 aaaatcctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt ttagatctat
    20941 taacgcttgg tggctgcttt ttttgggcga aaatagtcaa taatcttgtc cacttaccac
    21001 tcttttttgc actcttgaat tatggattat ggcttgcgat agcgttagtg attgtttggc
    21061 gaccctataa taaccctggt caacgccaaa tttttgcgat tatcgaatct attttcttta
    21121 cggataagaa ccactatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg
    21181 aggatgaata aatgcgagtc aaaggacaaa attcagttga taagaaatcc attttgcagt
    21241 ttggaaaacc agaagagcgc tctatcttgg acttatttcc atataaatcc tgtacagaag
    21301 agggagtctt aattactaga gaagatcatt ttcagcgtta ttatcgtgtc gtttccactg
    21361 acgtggaggg attaaatgaa caagagaaac tagaacgaat gaatcagttg accaccgtca
    21421 tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg gatctaacgg
    21481 aacaaatcgt gcaaaagcgg cagttattag ataaaaatcg tatggaacag tcactaggaa
    21541 gaaatctaca gcgattaaga aaatatgaaa agaaattagt agaggagatc caggaattaa
    21601 aacgggcaga acaagaacgt cctgacttaa gcttcttttt cgttattgaa ggaaagacaa
    21661 tcaaggattt acaaactaga aatcgtcagt taatgcgatc tagtggcatt ttagggttaa
    21721 aaccactcta caaaaaagaa ttgattaaga ttctttatcg aatgaacaac atgaatgacg
    21781 aataaaaaag atggaggaaa tgattcatgc tagatgtgat aaaaacagct ttttcaacgc
    21841 aaagtaatca aaaggtgatt gatccattaa aacaacttgg taatcgtgaa atagagattt
    21901 taaaatcaaa aggttatgat ttagatttct taaaacaagt gatgccacaa ggtgggttga
    21961 attttgatga agaatatgtg acctatggaa atggttattc acgatgtgtg acagtttatc
    22021 attacacaaa aaatcctact cttttttggt tggccaatct catgaacaat cctcatacag
    22081 tggccacact agatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaaccgcg
    22141 cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac cgaaataaaa
    22201 gtgcggaaga atacctggaa ctagcggaat atgcccgaaa attgacttct aatggggaaa
    22261 tttctaaaca agtcaaagtg agagtacatg tttacggaaa tacgatccaa gaagtggatg
    22321 aacgggcgca ggaactgatc gaaaatctaa aagggatgga ttatcgtgcg gtgacttatc
    22381 tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat
    22441 ggtttggaaa tatcaaaggg caatcacttc catcgttaaa cattggcgga ggattcccgt
    22501 tccaccacca aacgctaaaa gatccaggag gcacacaata tggggaaacc atgacaggcg
    22561 gcccatttgt ctggaatgcc acgtataaaa atagtgtacg tttatctacc aataccttgc
    22621 ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt
    22681 tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa
    22741 aggaatataa cggtattatg gtgcgtttag acggatctga cgggatgatc aatcctttag
    22801 agatctttgc cacacggacg tatgatgaag catccagttt atatgacgat ggttcagtca
    22861 aagatttgaa gatcaatgaa gcagcgtcct atcaaacgca cttagacaaa gtagtctatc
    22921 aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattc aagacctact
    22981 tgaatcgttt ttatgaagag tatggaacag taccacgcgg ttttacttcc tcaaattcgc
    23041 gttcaaatac tgatacgcaa gtcacaggga aagatccaga agcttatccg acgttcaaag
    23101 aatttttagc ctttttagga caactggaac taccaggggc ttcccaagag aaaaagaatc
    23161 ggaaagaaga aatggagtct atcgtggaat ccttatgtga gacttatggc atgatttttg
    23221 atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact
    23281 ctattacagg cctagatcga ggggtacaac aatgtcagat gtacatggcc atgacgttga
    23341 tttggaatca ggcattgatt caaggacgaa gacaacgacg cttaattaaa gaaaaacaaa
    23401 tggaagaaca agacttcaaa cgcttcttgg cgatgattga tgagtgccat aatgcgatta
    23461 atccagattt cattgaaacg gtaaattatg tcacaaactt ccaacgagaa atgcgtaaag
    23521 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    23581 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    23641 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacga
    23701 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    23761 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    23821 gaggaaaata atggaaaacc aaaatgaaag tttaatcaaa cagtatgtta agcgtcgtgc
    23881 aaagcggcgc ttatttttgt gggtgtttgg tacttctgca ggtttgatta ctatattgat
    23941 cactgtgttt gtgacccttt ttctaatact tgcggcaggt tctattgata attctgattc
    24001 aagtagtggt ggcgaagctt ttacaggaga atattcggaa ggcttaccca tttataaaga
    24061 aatcaaagga agaggccctt tttctgatga gattgcgcaa tatgctgtag gtgcagcagt
    24121 gaagtataaa ttattaccaa gtgtgatcct ttcgcaatac ggttatgaat cagcgtttgg
    24181 tacgtcggcg tccgcaagaa atgatctcaa ttattttggg attacgtggt ttgatgggtg
    24241 tctgtttcct aaaggaactg cgcgtggaat tggtggcatt gaaggtggct ggtacatgaa
    24301 gtttcccaac tcaaaagctg ccttctccta ttacggcttt atggtagcca cacaatcaaa
    24361 ttttaatgct tgtgtcggaa ataaaagccc tggcgcaagc ttattgatct taggacgagg
    24421 agggtatgcc gccgcaggaa ttactgaaga cagtgcctat tataaaaatt gtatgtctat
    24481 tattaataca aataaattga tggagtacga tgagtttgcg attaaacact ggagagaagg
    24541 cggcggaact ggtggtacga ttacaggaag ctggggcaat ccattcccag gaagtagctt
    24601 agataaaaat tcgttcagtg gaggacaact ttttgggaaa aatccaggag gagaattccg
    24661 acctaacggg ttccatgatg gactagactt tggttcagtc gatcatccag gaagtgagat
    24721 tcatgcggtt catggcggaa aagtcgtgta cgttggcaat cctggtattt caggtttggg
    24781 tgcgtgtgtg atcgtgatta attacgatgg cttgaatatg gtgtatcaag aatttgcaaa
    24841 tagtacagga aattctcgag tgaaagtagg cgatcaagtg aaagttggtc aagtaattgg
    24901 gatacgagat acagcacact tgcatttagg atttactcga atggattggc gtcaagcaca
    24961 aggacatgca ttcattgatg atggtacatg gatcgatccg ttaccatttt tgaatagcag
    25021 caaaaaatag atcaggagaa aaaatatgga aaataaaacc ttaaaaatcg tcttaacagc
    25081 tgcaattagc gttagtttaa tcggcaatat tgcgttgttt tggcagctta aagaagaaca
    25141 agagacaaat acctataaaa ttgaagaagt ggctaaaaac gcgtctgata agctcaaaaa
    25201 aaattcttct gaagcacagg cgaagattga caagttaaat caaaccatca atgagaaaaa
    25261 ccaagaaatc gatcagctga agcaacaaga tggcacaaaa gcaaaagagg aagtaactga
    25321 agcacaaaga aagacggcag aagagttcgg tcaattggcc attgataaat cattaagcag
    25381 aaatgaatta acggaaaaac taaaagatgt cgcaacccaa gaagtgatcg acaaattaag
    25441 tcctgtagac gatgatcacc aacatgatga ttacggaagt tactctttta cattaggtga
    25501 cgtgcaaact tacgctcaaa caagcagtgt aaaagaggaa caaaattttg tcgtatttgt
    25561 tgattataca atcggcaatc ctcaattcaa agatgtcaaa ccacaaaaaa taaaaggtgg
    25621 attaactgtc actgaaaaac aagtagatgg tcaatggaaa gtcacagact ttagttattt
    25681 cactcgttag gaggaagaaa atgagtaaga aaaaaggagc aagacaacgt tttaaaaaac
    25741 gaaaaagtta tcttagatgg ctggggatct ttatgattct cggctttttg attcttgcta
    25801 gtccgattta cacacataaa aaggtagaca taccagatga agtaagcacg aacgcatttt
    25861 taaacacgaa ggaactgacg atggtttcaa aagaaaaagg agcgaaccag caattagttt
    25921 ttgaatttgc gatcgacgat gaagatcaga gcttattaaa agaactagct aatcttaatt
    25981 atcgtgtaga agtaaagaca gctaaagggg attatcaagc aataaaagcc aaagtgatca
    26041 aggtgtctga tgattatttt gtagtgaagt tagcacatgt accagaagaa tatattgcta
    26101 tgcgattaac tattattcct gaaaaaattg atccaaaggt ggatatgcaa gaaccgcaag
    26161 atctgatttt ttatattcac gaagataagg tcaaagatca cgtgaaagac gaagattatg
    26221 aacaacatgc gatcaattac aaagtgaaag gatacaagaa agaacaaaaa gcagcacaaa
    26281 aacaaattga atccttccaa gcaaccattg atttgaatga agaattagtg aagaaactaa
    26341 aagatcaatt gccttatcaa gtcgcagaag atcaggaaaa tactgaaaat aagattaata
    26401 gttaccagca agaaattgaa acatcgaaaa cgcaaatgaa agatcaagaa gagagcattc
    26461 aaaaacttca agaaaaaatt gatcgtatga caaaagaaaa tatttagaag aataggtgga
    26521 gtaaaagaaa tgaagcgagc gaacttggtc ggcaaagata ctttcgctga gcagaaagta
    26581 ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcaaga agtgaattat
    26641 ctaacacaac aatatctgag ttgaagaatg gtagaaagag tttagatacc ttaaaattca
    26701 gatcatttga agctctatat atagctgcta aaaaatattt tacagctgat aataaataaa
    26761 aaattaaggt gggttctatg tgaaaaaaac gtatcaaaag tttttatgga ttagctatat
    26821 cgtcctattt atcgttgggt ttctcgtcag tgtgtccgtg atcgtcggaa cgctttttct
    26881 tttgttctct gacgaagatc tggtacttaa ggggttaatg agtacgttat cgtgtgtcat
    26941 gttactttat ttatgtatac agatcaacga aattatgccc cctcattggc aagaaaagaa
    27001 gaaagaattt aaggaagaaa tcgaaataaa atccaatgac tggattgaat acacatggct
    27061 tcctctgaaa agaaatagca aacgaaagga attttgggca ttgcaattat ggccaatagg
    27121 aaaacttttt atttatatgg cttcacttat ggttacttta atcggttatt tggtggtggt
    27181 ctatggggtg gcctattact tgttaagtga gccaaaacag atcgcggcgt caaatatttt
    27241 cacggcagta atgacattaa tgtgtgtcat gtgcttttct atttttagtt acttactcgt
    27301 ccaatttatc aggcaaaatt taacaatgag taaacttttc aaaagtttat tggacttact
    27361 tatcttaggc agtatgttaa cgactctaac agatcggttc gaacaatctg aaaaaacggt
    27421 agaggggata aagaacttct taacgacgat tgatcaattt ataaatggat atatgtattt
    27481 gtttgaagta ggctttattc tcactttgtt tggttgtgta tttataaaac tcattaaaag
    27541 atcaatcgaa aaagaaatgg ttattgatct aattccgacg ctgagcggct atgagtatta
    27601 cgttgatttt ggaaagtatg aggagttgat ccatacggat atcgttcaca cgagtatcca
    27661 tgaagtaacc gttaagggca tcagatctac tttaaaagat ggacaggaga cgaaaacaat
    27721 cgaccgaaga aaagaggtct acaattttta taaagttgaa gcaagtctac gatatggcat
    27781 tgggaaattg aggttagatg ggagtaatat tcaacaaaac aaaccatatg ttttaggaaa
    27841 agtggtgatt tagaacttcc tatgaaagat gaataaataa ggagaatctt ttgaaaatga
    27901 ttcttcttta cagaattgtg cttatagttg gcacggagtt aaaaatctta aagaaggagc
    27961 agttattcaa agtgaaagaa catcataatc agttactatg tattaaaacg ggtacgatga
    28021 acattcaata ccgaacagaa tatttagaat taacgaatca aactgatttt ttgtcgattc
    28081 gagagcaaca taagcaagaa aaattaatgg ctacttatag ccaattgatt gatgagtggg
    28141 aacaattcaa ggaatctaaa acataaaatt ccatttaaac accttatttt ttaaagagca
    28201 taaggaaacg tgtacccact ttcaaaataa agtgggtaga taggctgtgc ctatcacgtt
    28261 tctgaaaaaa ataaatgacc ccaaaatttt caatttatgt gtaggtttat gggtagcaaa
    28321 aatatgaatg tgggcagcaa aaagttcatt tgtgggtagt aaaattcaaa tgtgtgtagc
    28381 aatgtgtgta gctacacaca ttgctacaca cgctataaca tatagtgaaa aggagatatt
    28441 tatggaagta aaaataagag atattgatga aaaaacgatt caaagaattg accgaagatg
    28501 tattgaatta agtgaacgca ccgggactaa atggagccga aatcgatacc taaaagtttt
    28561 gattgaaaat gattttgacc atgaacttat ggatcataga aaagatcaat ttgatcggtt
    28621 gttagaaaaa tttgtggata ttcaaactta taatacgaaa attttagaag aatatattgc
    28681 aacaaataat caattaatcg ccttgttaat tgaataataa aaatagtatt gtgaatttat
    28741 aaaaggagga aatcaatttt ggcaaaagca acaagaaaac atgtatatct ttacgatatg
    28801 catatcgaag tacttgatga aataatttct actcatgaag atatatctaa ttatagtgaa
    28861 gcagtcagac atttgtgtat gatgtatgat ccaaaaatcg aagaaaaaat aaatccaatt
    28921 caaattaaat tgaatgcaat gggaaaagaa atttctatat taaccgaaat ggtttcagag
    28981 tttgcggatc ttcatttgaa agatacagga atactaattg gtacaaattc aactgtctat
    29041 caagaagcaa aagagcgtgt ggaagggaaa ataaaagcga atcaaacgaa aaagtattct
    29101 cctaagatca agaaaccaat aaatgaatct gttcatgcca gagtagaccc gtttgatcca
    29161 acaaagtttt taaaaagata aaaagtagaa ggggaacatg atgaataaat cgcctgcagt
    29221 cattctcact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat acattgatta
    29281 tatgacaaga gaaaaagcgc tggaagaaaa ggaacgacgc acgccggacg aagaacagga
    29341 actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc
    29401 ggagagtaag acagataaag aattcagtga aacgtcgaat gaagctcaat cgttgatgga
    29461 acatggaatc aattttgaca agttagagga acaagatttt accaaatatc tttcttatat
    29521 gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagggaac aaaaagaata
    29581 ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa aagcttcagg
    29641 aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg atctgaagga
    29701 gatcaaacaa atcttccaaa gagggcaaca aaaaggctca atcatttatc aagatgttgt
    29761 ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa cagatacctt
    29821 agatgaagaa ggattaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga
    29881 acaattgaat gaaacaggtt actggatggc aacgattcac cgtaacacta agcatatcca
    29941 tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa
    30001 tggtgtgagt tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa
    30061 gaccaattat gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat
    30121 ggagaaacga aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt
    30181 aaaagagcca gcgcgttacg atcgacacgc aatgaactta ttaaatgaag tatataaaac
    30241 cctacctgac aaacgttcgg aatggcatta tggggatgaa aaacgaacca aactttcacc
    30301 atacacaaga gagaaactcg atgagttaac caaatatgtg ttaacaaaag aacctgaata
    30361 tcaagaatat atacagattt caaaatcatt gaaagaagaa gcaaaaagtc tttatgggga
    30421 aagcaagaga gaatcaaagg atgcagaaaa aaatgctctc tttgatttga agaagcgcac
    30481 aggaaatgcc attctaacag aactgaagaa acaggatagt caaataaaac ctttaataga
    30541 aagtatggat cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacagtt
    30601 accaacgaat gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca
    30661 acaaagagtt gtcacgcaac ggagtttgcg tcaattaaat cgaacgatta atgcccatga
    30721 agaaaaatac cacgcaagta aagcctacga agagcaacaa cgaagaattc aaatggaaca
    30781 agaaagacag taaagacagt aggaggaata gcaatgaaac aatatttaga tcaatggaaa
    30841 gttatagaag gatcattaag agaagaacga atcgaacaac ttcccgattg tttagaaaaa
    30901 gagcatttgt ttcagataag agaaatgttg agaaacgaac agtttgatcc aaatcaattt
    30961 ttagtcgttg agtactcagc tactggtgtg tactgttgta atcatgtgaa gggtgaaaaa
    31021 tattttatca ttcaagaata tgagggcaag ttagcgcctt attatactac ttgggaaatg
    31081 aatgaagagg ggatcaataa cttcccgtgt aaaagcattg aagagtctat ttcactcaca
    31141 gaatgttagt tgttaagtga ataaaagaag taaaagagtc ctgaacgtaa ttgtccagga
    31201 ctcttttttt caatgaattg ttttccgcca attcaatgag tggtgcgatt tgcgactaca
    31261 ttatatcaaa agaaagagaa gggaggaaga agaaatggct tattcaacga ttggagttgt
    31321 tgttgaaaaa agcagagaca atctcgtttt tgtgacagaa attcaaacgg gcagagcttt
    31381 tgtcgtcaca gacaaagcgg caaaagcgta tcaaagtggc gatattttaa cgttaaatct
    31441 gactacgaag acgttcgtcg atgcggcaga agattatcca tt
//
LOCUS       DAFELO010000030        30348 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFELO010000030
VERSION     DAFELO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.030"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            170..514
                     /locus_tag="LP452_001458"
     CDS             170..514
                     /locus_tag="LP452_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL2014190.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LP452_001459"
                     /pseudo
     CDS             515..934
                     /locus_tag="LP452_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="LP452_001460"
     CDS             complement(1026..1376)
                     /locus_tag="LP452_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014191.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LP452_001461"
     CDS             complement(1342..2673)
                     /locus_tag="LP452_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL2014192.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LP452_001462"
     CDS             2929..3495
                     /locus_tag="LP452_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2014193.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LP452_001463"
     tRNA            3640..3727
                     /locus_tag="LP452_001463"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="LP452_001464"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="LP452_001464"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL2014194.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="LP452_001465"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="LP452_001465"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL2014195.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNETIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="LP452_001466"
     CDS             complement(7450..7896)
                     /locus_tag="LP452_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2014196.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="LP452_001467"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="LP452_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2014197.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="LP452_001468"
     CDS             8361..8879
                     /locus_tag="LP452_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2014198.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="LP452_001469"
     CDS             complement(8956..9912)
                     /locus_tag="LP452_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2014199.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="LP452_001470"
     CDS             10078..10884
                     /locus_tag="LP452_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014200.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="LP452_001471"
     CDS             10881..11870
                     /locus_tag="LP452_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL2014201.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="LP452_001472"
     CDS             11867..12871
                     /locus_tag="LP452_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL2014202.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="LP452_001473"
     CDS             13003..13545
                     /locus_tag="LP452_001473"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL2014203.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="LP452_001474"
     CDS             13565..14263
                     /locus_tag="LP452_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL2014204.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="LP452_001475"
     CDS             14284..14496
                     /locus_tag="LP452_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL2014205.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="LP452_001476"
     CDS             14496..15458
                     /locus_tag="LP452_001476"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL2014206.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="LP452_001477"
     CDS             15476..15871
                     /locus_tag="LP452_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2014207.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="LP452_001478"
     CDS             16034..16399
                     /locus_tag="LP452_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL2014208.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="LP452_001479"
     CDS             16396..18207
                     /locus_tag="LP452_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL2014209.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="LP452_001480"
     CDS             complement(18269..18436)
                     /locus_tag="LP452_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL2014210.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="LP452_001481"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="LP452_001481"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL2014211.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="LP452_001482"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="LP452_001482"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL2014212.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="LP452_001483"
     CDS             21193..22173
                     /locus_tag="LP452_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2014213.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22364..23086
                     /gene="tsaB"
                     /locus_tag="LP452_001484"
     CDS             22364..23086
                     /gene="tsaB"
                     /locus_tag="LP452_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL2014214.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="LP452_001485"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="LP452_001485"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL2014215.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="LP452_001486"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="LP452_001486"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL2014216.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="LP452_001487"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="LP452_001487"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL2014217.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25705..26406)
                     /locus_tag="LP452_001488"
     CDS             complement(25705..26406)
                     /locus_tag="LP452_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2014218.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            26867..28096
                     /gene="arcA"
                     /locus_tag="LP452_001489"
     CDS             26867..28096
                     /gene="arcA"
                     /locus_tag="LP452_001489"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL2014219.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            28187..29206
                     /gene="argF"
                     /locus_tag="LP452_001490"
     CDS             28187..29206
                     /gene="argF"
                     /locus_tag="LP452_001490"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL2014220.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            29321..30268
                     /gene="arcC"
                     /locus_tag="LP452_001491"
     CDS             29321..30268
                     /gene="arcC"
                     /locus_tag="LP452_001491"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL2014221.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         9921 a         5198 c         6316 g         8913 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaaa cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaaaaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttgttcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 atacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcagcc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatatc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgcttta agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat cttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaatcgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaatgaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctggatgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatttatcag
    25681 ggtcactgct tatttttgtt cgcgttattc tctcatagct ggagtagtag ctgtcttaca
    25741 gaaagtaaaa cgctttttgt cataatctaa cagcccggaa tcacgtagtt gtttgattac
    25801 ttgtccagca gtttcacgtg tcgtgccgct attcactgct aattctttta aggtgatcgg
    25861 ataaggaatg gtgatatgac catcttttgt tggtacgccc aattcttctt tcaaaatgaa
    25921 taaattttgt tttacccggt caaaggcgct agaggtcaat ccgatttgga tacgcaattc
    25981 atgtatttca actactttag aaattttttg tatcatatag atcaactgtt cgggattttt
    26041 tcgtgctaat ttttcatagt aggaaacagg aaggtagaat gtttcaatat cagtcatcgc
    26101 ttgagctgta taagtgtagg tctcttctcc aaacattcct gacataggaa aaaggttatt
    26161 tgtattcaca tagtcatagt agtaataagt tgcactttca tcataacgtt ctaaacgaac
    26221 gagtcctttg gtcaagatat acaaccgttg tctgggatca ccatcgtcga aaaggatctg
    26281 tcctttttta taagaacgaa gaacagtatg ttccttgatc tgttggaact cttcatccga
    26341 gaaatgttca aaatcaggtt gatatctgag ttttgaaaag tgaaaatcga ttttattaac
    26401 acgcattaaa aagtcctctc cttattgttc actatattta ttgtcacttt ttttaaagaa
    26461 tttgtaaagt tttaacatac caacattaga aacaagtttt aatactttac aaatataatt
    26521 tgttattatg taaaaaatat acaatggtat tgaataattt gttttgaagc gaatgatttg
    26581 tgtataaaaa acaacaataa tacatatagt aaacgctaac aacgataata taatttattt
    26641 ttttaattta ttcaaaaaaa ttttttcaat gaataaatat ttttttgaaa gtgtatacag
    26701 aataaaaaat gaataaatta cgcttaaaaa tagaaaaacg tttaattatt taattatcga
    26761 atattaatgt gaggaatgtc tcattttgaa tgagacaaaa ttatgaaagt gattacaaaa
    26821 ggaggatatc ctatacatgt aatcaaaaag aaggaggcaa cacaacatgg ataaacctat
    26881 tcacgttttc tctgaaatag ggaaattaaa gacagtttta ctgaaacggc ctggacaaga
    26941 agtggaaaat ttgacaccag atattatgga tcgtctgttg ttcgatgata ttccatatct
    27001 tccgattgct caagaagaac atgacaactt tgcgaagaca ttgcaaaatg aaggcgtcga
    27061 gactctttac ttggaaaaat tagcggcaga agcgattgac gcaggcaatg tcaaagaaca
    27121 attcttgaat aaaatgttgg atgaatctca tattgcatcg aatgctgtaa gagagggtct
    27181 tcatgagttc cttctatcaa tggaaacgca agaaatggta gataaaatca tggctggggt
    27241 tcgtacaaaa gatatcgaag tacgttctag cagtctatat gatctgtcag cagacgatga
    27301 ctatccattt tacatggacc caatgccaaa cttgtacttt acacgcgacc cttccgcatc
    27361 aatgggaaat gggatgacag tcaataagat gacttttgaa gcacgtcgtc gtgaatcgat
    27421 gtttacagaa tatattttga aacatcatcc aagatttgca aataaaggaa tcgaagtatg
    27481 gttggatcgt gaaaatccag atcatatcga aggcggagac gaattgatct taagcgataa
    27541 agtagtagct gttgggattt ctcaacgaac taatgcgaaa gctttagaaa aattagcacg
    27601 ccacctattc gcaaaaaatt caggatttga aaaagtgttg gcaatcaaaa ttccgaacaa
    27661 ccgtgcaatg atgcatttgg ataccgtatt tacaatggta gatcacgata aattcactat
    27721 ccatccagca attcaaagta aagacggtaa gatggatgtc tttacaatcg aaccagatgg
    27781 tgatgatatc aagattactc attcagataa cttacacgaa acgatgaaag ctgctcttgg
    27841 attagatgat cttgtactga tcccaactgg taatggagat gagatcgttg cacctcgaga
    27901 acaatggaat gatggatcaa acacattggc aatcgctcca ggtgttgtag tcacttataa
    27961 ccgaaattat gtatcgaacg aattgcttcg tagctatggc atcaaagtgt tagaaatcaa
    28021 ttcaagtgaa ttgtcacgag gtcgtggtgg tccacgctgc atgagccaac cacttgtccg
    28081 tgaagacttg aagtaatttg tttcctgacg gcagtcttac tgtcagctgc cgtcgggatt
    28141 tcctctttaa tcttaaataa aaacaatcta aaggagaatg tttaatatga aagaatcagt
    28201 attccaagga agaagcttat tagcagaaaa agactttaca aaagaagaat tacaatattt
    28261 gatcgatttt tcagaacact tgaaagactt gaaaaaacgt ggtatcccac atcactatct
    28321 agaaggaaaa aatattgcgc ttttatttga aaaaacatct acacgtactc gttctgcatt
    28381 tactactgca gcgatcgatc taggtgcaca tcctgaatat ctaggtgcaa atgatatcca
    28441 attaggtaaa aaagaatcca cagaagatac agctaaagta ctaggacgta tgtttgacgg
    28501 aatcgaattc cgtggattca gtcaaaaaat ggtagaggaa ctagcagaat tctctggtgt
    28561 tccagtttgg aatggcttga ctgatgaatg gcatccaaca caaatgatcg ctgacttttt
    28621 aactatccaa gaaaactttg gaacagtaga aggtatcact gttgcttact gcggagatgg
    28681 tcgaaacaat atggctaact ctttattagt cacaggtgca attttaggag cgaatatgcg
    28741 tatcgttgca ccaaaagaat tacaacctga agaagaaatc gtcaaaatgg cagaaggctt
    28801 tgctgaaaaa tccggtgcac aattgatgat taccgacgat gttgataaag gtgtagacgg
    28861 agcagacgtc ctttattctg atgtatgggt atcaatgggt gaagaagata aattcgaaga
    28921 acgtatcaaa ttgttgaaac cttaccaaat caatatggaa atggtagaaa aaacacacaa
    28981 cacagatcgt ttgatcttct tgcactgctt gccagctttc catgatacaa acactgttta
    29041 cggtgaacaa atgaaagaac gctttggcat cactgaaatg gaagtaacag atgaagtctt
    29101 ccgcagcaaa tatgctcgtc aatttgacca agcagaaaat cgtatgcact caatcaaagc
    29161 gatcatggca gcaacattag gtaacttgtt tatccctcgc gtttaagcat aaccacattc
    29221 atctcacgaa aaaacgagtc agcattgagg gaaaatcaca aaacgctggc tcgttttgtt
    29281 atctagaaaa ttcggtagaa ctgaagaaag gacgaatgaa atggccaaac gaaaagtagt
    29341 tgtcgcttta gggggcaatg ccatcttgtc tacggatgca agtgcaaaag cccaacaaga
    29401 agcgttaatg gaaacagcaa agtatttagt gaaatttatc gaacaaggag atgaattgat
    29461 catctcccac ggaaatggtc cgcaagtcgg taacctattg attcagcagc aagctgctga
    29521 ttctgagaaa acaccggcaa tgcctttaga tacatgtgtt gcaatgacag aaggatctat
    29581 cggttactgg ttacaaaacg ccatgggcga agtactgaag gaaaaaggga tcgataaaga
    29641 tgtggtttct ttagtcactc aagttattgt ggatgaaaat gatccttcat tcaaaaatcc
    29701 aagtaaacca gtcggtccat tctacacgga agaagaagcc aaagaacaaa tgaatgctga
    29761 ctccactgtg acattcaaag aagatgctgg acgtggatgg agaaaagtag tcgcttcccc
    29821 taaacccatc tcgattaaag aagcacgtgt catcgaaacg ctggtcgatc aaggagttat
    29881 cacagtctct gttggtggcg gtgggattcc tgttgtagaa acagctactg gattagaagg
    29941 acgagaagcc gtaatcgata aagattttgc ttcagaaaaa ttagccgaaa tcatcgatgc
    30001 ggatttgctg atcgtattga caggagtaga caatgtgtat gtcaattacc aaaaaccaga
    30061 tcaaaagaaa ttagaaactg ttactgtttc agaaatgaaa caatacattg atgaaaagca
    30121 attcgctcca ggaagcatgc ttccgaaagt agaagcagct attgcatttg ttgaagcaaa
    30181 accaaacgct aaggcaatca tcacttcttt agaaaatatc gagaatcttt tagcttctga
    30241 agaaggaacg attatcgtag ctgattaatt tgttaagtct ttcgtattta cgaaatgatt
    30301 tatagctatc caggtggtat ttcttctcct ttaggccatc tggatttt
//
LOCUS       DAFELO010000031        28994 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFELO010000031
VERSION     DAFELO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.031"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(53..577)
                     /locus_tag="LP452_001492"
     CDS             complement(53..577)
                     /locus_tag="LP452_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014222.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(570..1043)
                     /gene="tsaE"
                     /locus_tag="LP452_001493"
     CDS             complement(570..1043)
                     /gene="tsaE"
                     /locus_tag="LP452_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL2014223.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1166..2146)
                     /gene="pta"
                     /locus_tag="LP452_001494"
     CDS             complement(1166..2146)
                     /gene="pta"
                     /locus_tag="LP452_001494"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL2014224.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2299..2979)
                     /locus_tag="LP452_001495"
     CDS             complement(2299..2979)
                     /locus_tag="LP452_001495"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL2014225.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3105..3974
                     /locus_tag="LP452_001496"
     CDS             3105..3974
                     /locus_tag="LP452_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2014226.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4000..4515
                     /locus_tag="LP452_001497"
     CDS             4000..4515
                     /locus_tag="LP452_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014227.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4562..5155)
                     /locus_tag="LP452_001498"
     CDS             complement(4562..5155)
                     /locus_tag="LP452_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014228.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5180..5359)
                     /locus_tag="LP452_001499"
     CDS             complement(5180..5359)
                     /locus_tag="LP452_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014229.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5436..5945)
                     /locus_tag="LP452_001500"
     CDS             complement(5436..5945)
                     /locus_tag="LP452_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014230.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6041..6421)
                     /locus_tag="LP452_001501"
     CDS             complement(6041..6421)
                     /locus_tag="LP452_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2014231.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6751..7500
                     /locus_tag="LP452_001502"
     CDS             6751..7500
                     /locus_tag="LP452_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL2014232.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7684..8961)
                     /locus_tag="LP452_001503"
     CDS             complement(7684..8961)
                     /locus_tag="LP452_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014233.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9040..9684)
                     /gene="pcp"
                     /locus_tag="LP452_001504"
     CDS             complement(9040..9684)
                     /gene="pcp"
                     /locus_tag="LP452_001504"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL2014234.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9883..10827)
                     /locus_tag="LP452_001505"
     CDS             complement(9883..10827)
                     /locus_tag="LP452_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL2014235.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10829..11515)
                     /locus_tag="LP452_001506"
     CDS             complement(10829..11515)
                     /locus_tag="LP452_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL2014236.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            11989..12309
                     /locus_tag="LP452_001507"
     CDS             11989..12309
                     /locus_tag="LP452_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL2014237.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12463..13368
                     /locus_tag="LP452_001508"
     CDS             12463..13368
                     /locus_tag="LP452_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2014238.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13365..14138
                     /locus_tag="LP452_001509"
     CDS             13365..14138
                     /locus_tag="LP452_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2014239.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14319..15743)
                     /locus_tag="LP452_001510"
     CDS             complement(14319..15743)
                     /locus_tag="LP452_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2014240.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            15954..16400
                     /gene="nrdI"
                     /locus_tag="LP452_001511"
     CDS             15954..16400
                     /gene="nrdI"
                     /locus_tag="LP452_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2014241.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16465..18447)
                     /locus_tag="LP452_001512"
     CDS             complement(16465..18447)
                     /locus_tag="LP452_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2014242.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18461..19357)
                     /gene="murQ"
                     /locus_tag="LP452_001513"
     CDS             complement(18461..19357)
                     /gene="murQ"
                     /locus_tag="LP452_001513"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL2014243.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19360..20421)
                     /locus_tag="LP452_001514"
     CDS             complement(19360..20421)
                     /locus_tag="LP452_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2014244.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20632..21198)
                     /locus_tag="LP452_001515"
     CDS             complement(20632..21198)
                     /locus_tag="LP452_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2014245.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21226..22728)
                     /gene="eat(A)"
                     /locus_tag="LP452_001516"
     CDS             complement(21226..22728)
                     /gene="eat(A)"
                     /locus_tag="LP452_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL2014246.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(22986..24107)
                     /locus_tag="LP452_001517"
     CDS             complement(22986..24107)
                     /locus_tag="LP452_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2014247.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24233..25174
                     /locus_tag="LP452_001518"
     CDS             24233..25174
                     /locus_tag="LP452_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2014248.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25324..26220)
                     /locus_tag="LP452_001519"
     CDS             complement(25324..26220)
                     /locus_tag="LP452_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL2014249.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26489..27829
                     /locus_tag="LP452_001520"
     CDS             26489..27829
                     /locus_tag="LP452_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2014250.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28047..28490
                     /locus_tag="LP452_001521"
     CDS             28047..28490
                     /locus_tag="LP452_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL2014251.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT         8350 a         6002 c         5210 g         9432 t
ORIGIN      
        1 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
       61 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
      121 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
      181 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
      241 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
      301 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
      361 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
      421 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
      481 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
      541 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
      601 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
      661 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
      721 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
      781 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
      841 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
      901 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
      961 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     1021 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     1081 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     1141 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     1201 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     1261 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     1321 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     1381 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     1441 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     1501 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     1561 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     1621 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     1681 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     1741 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     1801 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     1861 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     1921 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     1981 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     2041 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     2101 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     2161 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     2221 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     2281 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     2341 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     2401 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     2461 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     2521 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     2581 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     2641 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     2701 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     2761 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     2821 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     2881 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     2941 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     3001 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     3061 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     3121 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     3181 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     3241 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     3301 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     3361 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     3421 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     3481 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     3541 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     3601 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     3661 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     3721 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     3781 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     3841 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     3901 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     3961 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     4021 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     4081 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     4141 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     4201 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     4261 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     4321 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     4381 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     4441 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     4501 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     4561 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     4621 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     4681 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     4741 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     4801 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     4861 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     4921 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     4981 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     5041 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     5101 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     5161 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     5221 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     5281 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     5341 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     5401 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     5461 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     5521 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     5581 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     5641 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     5701 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     5761 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     5821 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     5881 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     5941 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     6001 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     6061 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
     6121 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
     6181 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
     6241 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
     6301 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
     6361 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
     6421 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
     6481 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
     6541 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
     6601 aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa
     6661 agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac
     6721 tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat
     6781 cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct tggctgccag
     6841 gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc
     6901 acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta
     6961 ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt
     7021 atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa
     7081 tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg gtttatcgca
     7141 atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat
     7201 gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc
     7261 agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg
     7321 gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc
     7381 tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa
     7441 gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa
     7501 aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     7561 aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
     7621 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctctat
     7681 tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc
     7741 taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc
     7801 tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg
     7861 ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc
     7921 tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc
     7981 ttctgtaaaa ccatagtgac aatattgctt ccaaataaat agatagattt ttggttggat
     8041 gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg
     8101 ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac
     8161 ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa
     8221 ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg
     8281 tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc
     8341 ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc
     8401 taattttata tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca
     8461 gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc
     8521 gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga
     8581 gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg
     8641 cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg
     8701 gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc
     8761 agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac
     8821 tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta
     8881 ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa
     8941 gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac
     9001 taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt
     9061 tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc
     9121 aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga
     9181 gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag
     9241 caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg
     9301 tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc
     9361 ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat
     9421 tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc
     9481 ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg
     9541 actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc
     9601 atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc
     9661 aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt
     9721 tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata
     9781 acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag
     9841 aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat
     9901 ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc
     9961 gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg
    10021 tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt
    10081 cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc
    10141 aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc
    10201 gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac
    10261 tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg
    10321 ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg
    10381 agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc
    10441 acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat
    10501 aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg
    10561 gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc
    10621 accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca
    10681 gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt
    10741 gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa
    10801 aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg
    10861 ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca
    10921 ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg
    10981 ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc
    11041 agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt
    11101 ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc
    11161 cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa
    11221 tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt
    11281 ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca
    11341 tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac
    11401 cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa
    11461 atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt
    11521 ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt
    11581 tgttttctca cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa
    11641 attatcttac ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa
    11701 ataaggtcta gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa
    11761 ttgcaaagca ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt
    11821 actaaaaagt atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat
    11881 acaatctgcc accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg
    11941 attttcctct ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg
    12001 gaattaatca tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat
    12061 ggtttttcta aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac
    12121 tttttgtcaa aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg
    12181 attggcgcag ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt
    12241 tccacttggt tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc
    12301 ggccactaga cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt
    12361 caattttttt atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc
    12421 aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc
    12481 caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta
    12541 tcccctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg
    12601 aaagctattt taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag
    12661 atttctccct ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt
    12721 atctatccat tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta
    12781 tctgctatcg acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg
    12841 aaatcctact cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat
    12901 ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat
    12961 gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac
    13021 ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc
    13081 atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct
    13141 acaacaaatg atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat
    13201 cctgataaaa tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta
    13261 ttgtctgcac aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct
    13321 tctctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa
    13381 aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag
    13441 ttgttttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag
    13501 ttgaaatgac tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag
    13561 ctgcaagcac gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc
    13621 gaaagtattc tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg
    13681 tttatttata ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt
    13741 cgatttcctt cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt
    13801 atactttggg tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt
    13861 gctttatcaa tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt
    13921 caatcatatc tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc
    13981 cgatcagtat gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga
    14041 ctcatctttc tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag
    14101 cattaggata tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg
    14161 tgaaagagac gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa
    14221 aaaggagctg tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg
    14281 acaggctcac aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg
    14341 gcaaataaaa ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct
    14401 aagtggatat gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg
    14461 cctggcaaga aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc
    14521 catacttgaa aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga
    14581 taagaaagtt tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta
    14641 ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca
    14701 ttcgtacata acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt
    14761 ccccatgctt taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag
    14821 atcgtccatg agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact
    14881 gctccccatt taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc
    14941 gtttgcagct gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata
    15001 ataatcaagc cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct
    15061 cgtgaaccta cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg
    15121 caactcaata cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg
    15181 ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat
    15241 aaaaataatg gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg
    15301 actccccgat tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat
    15361 gccagatttt gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat
    15421 gttgcaaaag ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa
    15481 ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga
    15541 cgttttttat tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt
    15601 acccaactga aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat
    15661 acgccacctc caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct
    15721 tttttctgtt gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa
    15781 agtataacaa acctttttta tttttactta attaataaaa actttaatag aaatataatt
    15841 ctaccggctt ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac
    15901 caaaaaactt aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca
    15961 ttttatatat atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg
    16021 ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg
    16081 aaaatagtga ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag
    16141 acggcggaaa tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat
    16201 atctggaaca tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa
    16261 acttcaataa tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt
    16321 tcttggcaga ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt
    16381 tagcagaaaa caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct
    16441 ttcgtttcta ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt
    16501 tgctttacct gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac
    16561 gatcatggat gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag
    16621 cgtatcaggt gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa
    16681 gtcaactgtg ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc
    16741 aatagcgtgt ttcgttggga agacagagct gactacccca gtaactggag aatagatatg
    16801 tccgttttct ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc
    16861 acttgcttta tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact
    16921 ttcagctgaa gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt
    16981 cgctaattct tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa
    17041 gccaccagca taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat
    17101 caatgggatc aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat
    17161 tccgatcact gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag
    17221 aggtaatgtt actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc
    17281 tcctttgatc atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc
    17341 tgcacccact tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt
    17401 ttcatcaatc atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat
    17461 cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca
    17521 gttgatccca ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat
    17581 ttcaatcaga ccgatggcaa tcaacgaaag agttggtgta acgatgatat ctaccgcatc
    17641 aggtactact ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac
    17701 acctaggatc ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg
    17761 tgcttccgga ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg
    17821 tgttgcacca aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat
    17881 cccgtttttc aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt
    17941 tccggctgtg actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac
    18001 aaatgcagga atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt
    18061 agatggtttt tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg
    18121 gtcttttcca gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc
    18181 taatttcacg cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc
    18241 gatgatgact tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa
    18301 ttcagagaca tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg
    18361 gacaatgctt tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc
    18421 ttccaaagtc agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa
    18481 atccgtttgc ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca
    18541 tgacgatagc cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt
    18601 aatcagcttc tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg
    18661 ttggtttgac atcgaccatc aagttattga atactttacc tataccaatc atactgatag
    18721 tagaaatcat attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg
    18781 tcaaaaattc tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg
    18841 cttgtttatt gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc
    18901 caccgatcac atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg
    18961 tcaaattcag cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga
    19021 cagctacagt cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta
    19081 caccgaatgt cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg
    19141 ctcccatata aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg
    19201 gttcgatact tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca
    19261 ttagttgaac ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc
    19321 gttccgttgt taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat
    19381 aaattgatag ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac
    19441 tttagctact acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc
    19501 acccatatag cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc
    19561 tgtatttctt gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac
    19621 atctcttgca tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc
    19681 atctacaggt tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc
    19741 ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa
    19801 tagatctaga gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc
    19861 ttgatatagt gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc
    19921 ttgaattttg agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg
    19981 acgtggataa taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa
    20041 ttcatcgata tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga
    20101 ccagtcggca atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat
    20161 tgctttcaaa ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga
    20221 aatatcgacc atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg
    20281 cttttgatat gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa
    20341 gccattggat gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat
    20401 aaatattgaa aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga
    20461 ctagagtata tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca
    20521 tttctaaagg ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct
    20581 aagcaaaagc agtgccttga aatattttat ttaaataaca caaaaacata attactcata
    20641 agaaaccaat tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg
    20701 aaaataagca ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc
    20761 ttcgtcttcg atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga
    20821 ctccccattt gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt
    20881 gcttggcagt ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag
    20941 cggcaagctg agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga
    21001 atggatgatt ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc
    21061 ctgcatctgt cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc
    21121 gttccaagtt gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc
    21181 aaagttcccc ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag
    21241 agaaataatc tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat
    21301 ggcaggtttc acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata
    21361 attcaatggt tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc
    21421 cacttttttc cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc
    21481 catcccaagc tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc
    21541 aaattccgcc aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag
    21601 atgttttggc tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg
    21661 gataatggat gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc
    21721 gattgtaaaa tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac
    21781 gcttaaaagt cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc
    21841 gatatctttt aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc
    21901 tttggatcgt ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct
    21961 agattcagta tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga
    22021 ccattcggct ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc
    22081 attttgagcc atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt
    22141 cccttgataa agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc
    22201 gataaatccc cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc
    22261 cgctacttgt tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa
    22321 atgagtgtca tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag
    22381 tgtgctgaat tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc
    22441 gatttcccat atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt
    22501 atcttttgtc tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata
    22561 aggtagtttg ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat
    22621 aagtcctagt ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc
    22681 ttggctgtcg tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct
    22741 cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac
    22801 acacaaaaag acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt
    22861 ccttgtctcc acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat
    22921 gcacggatcc ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt
    22981 gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt
    23041 ttttctaatg tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata
    23101 cttaagacaa ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa
    23161 atagcgatta ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt
    23221 ccgtggatcg tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg
    23281 atcttttttt ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct
    23341 cccaacaaaa aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct
    23401 aaatccacct cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag
    23461 aaagctagcg tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt
    23521 tgacctgtat atggattggt aacatctgca gcgataatca attttacttt agaaacaaga
    23581 acgtttcttc gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac
    23641 ccttctaaag aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt
    23701 cctcctaaag tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct
    23761 cctaaggcat aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct
    23821 aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat
    23881 aaatagctga tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt
    23941 cccctgagcg cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca
    24001 ttgatttttc ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat
    24061 gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac
    24121 tttcttctta agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt
    24181 ccatttttct atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat
    24241 cgcaatagct ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg
    24301 aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg
    24361 gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga
    24421 agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca
    24481 attagatgat atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg
    24541 tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat
    24601 cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt
    24661 aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa
    24721 agttgtagaa gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta
    24781 ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga
    24841 ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt
    24901 atctgaattt gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta
    24961 ccaacatatt gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta
    25021 ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg
    25081 gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga cccaattagt
    25141 ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga
    25201 atcagcagct ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct
    25261 catgatgcca taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt
    25321 tttttatttg ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc
    25381 gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag
    25441 gattggctta aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag
    25501 caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac
    25561 tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc
    25621 ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt
    25681 aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc
    25741 tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc
    25801 accaaaaaat agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa
    25861 tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc
    25921 atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg
    25981 attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc
    26041 catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc
    26101 tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat
    26161 ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat
    26221 cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa
    26281 gccagtaaaa cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga
    26341 aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg
    26401 aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata taaattttga
    26461 ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag
    26521 ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg
    26581 aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg
    26641 gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac
    26701 gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac
    26761 tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc
    26821 ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc
    26881 tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg
    26941 gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt
    27001 cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg
    27061 tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc
    27121 ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc
    27181 gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt
    27241 gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt
    27301 tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat
    27361 tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt
    27421 cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca
    27481 agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc
    27541 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt
    27601 attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt
    27661 atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat
    27721 cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta
    27781 tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa
    27841 aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag
    27901 tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc
    27961 aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag
    28021 gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg
    28081 gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata
    28141 tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca
    28201 tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag
    28261 ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca
    28321 acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta
    28381 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
    28441 aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg
    28501 tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta
    28561 cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    28621 cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc
    28681 aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag
    28741 agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    28801 cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta
    28861 agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa
    28921 actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc
    28981 cctttttatg tttt
//
LOCUS       DAFELO010000032        28140 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFELO010000032
VERSION     DAFELO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.032"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(82..777)
                     /locus_tag="LP452_001522"
     CDS             complement(82..777)
                     /locus_tag="LP452_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL2014252.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="LP452_001523"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="LP452_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL2014253.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="LP452_001524"
     CDS             complement(1976..2167)
                     /locus_tag="LP452_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL2014254.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="LP452_001525"
     CDS             complement(2188..3078)
                     /locus_tag="LP452_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL2014255.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="LP452_001526"
     CDS             complement(3065..3832)
                     /locus_tag="LP452_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2014256.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="LP452_001527"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="LP452_001527"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL2014257.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="LP452_001528"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="LP452_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL2014258.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="LP452_001529"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="LP452_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL2014259.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="LP452_001530"
     CDS             8403..8786
                     /locus_tag="LP452_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014260.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /locus_tag="LP452_001531"
     CDS             complement(8954..9712)
                     /locus_tag="LP452_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL2014261.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="LP452_001532"
     CDS             complement(9730..10548)
                     /locus_tag="LP452_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL2014262.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="LP452_001533"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="LP452_001533"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL2014263.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="LP452_001534"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="LP452_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL2014264.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="LP452_001535"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="LP452_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL2014265.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="LP452_001536"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="LP452_001536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL2014266.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="LP452_001537"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="LP452_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL2014267.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="LP452_001538"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="LP452_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL2014268.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="LP452_001539"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="LP452_001539"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL2014269.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="LP452_001540"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="LP452_001540"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL2014270.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /locus_tag="LP452_001541"
     CDS             20590..20889
                     /locus_tag="LP452_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL2014271.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="LP452_001542"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="LP452_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2014272.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /locus_tag="LP452_001543"
     CDS             21477..21713
                     /locus_tag="LP452_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL2014273.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="LP452_001544"
     CDS             21856..23829
                     /locus_tag="LP452_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL2014274.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="LP452_001545"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="LP452_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL2014275.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="LP452_001546"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="LP452_001546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL2014276.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26708..28000
                     /locus_tag="LP452_001547"
     CDS             26708..28000
                     /locus_tag="LP452_001547"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL2014277.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8574 a         5515 c         5591 g         8460 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtact taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agaccaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26161 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26221 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26281 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26341 atctgcggta atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt
    26401 cttattactt gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta
    26461 gcttcttcga aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc
    26521 cttcttattt cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa
    26581 aaagataaag ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt
    26641 atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg
    26701 tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa
    26761 aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa
    26821 tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg
    26881 gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc
    26941 attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat
    27001 ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga
    27061 tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga
    27121 taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga
    27181 acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga
    27241 accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca
    27301 atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt
    27361 gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac
    27421 ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat
    27481 caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc
    27541 aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac
    27601 gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa
    27661 acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga
    27721 aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc
    27781 aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga
    27841 agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg
    27901 tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac
    27961 acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa
    28021 tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa
    28081 cactgtttat tcagaataag ggactgtgac aaaaaccgtg tcacagtccc tttttccgaa
//
LOCUS       DAFELO010000033        28126 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFELO010000033
VERSION     DAFELO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.033"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LP452_001548"
     CDS             6..305
                     /locus_tag="LP452_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2014278.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LP452_001549"
     CDS             378..1739
                     /locus_tag="LP452_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2014279.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LP452_001550"
     CDS             1761..2021
                     /locus_tag="LP452_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014280.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LP452_001551"
     CDS             2035..2787
                     /locus_tag="LP452_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL2014281.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LP452_001552"
     CDS             complement(2881..3486)
                     /locus_tag="LP452_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2014282.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LP452_001553"
     CDS             complement(3539..4513)
                     /locus_tag="LP452_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL2014283.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LP452_001554"
     CDS             complement(4891..5610)
                     /locus_tag="LP452_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2014284.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP452_001555"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP452_001555"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL2014285.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LP452_001556"
     CDS             7161..8558
                     /locus_tag="LP452_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2014286.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LP452_001557"
     CDS             8639..9079
                     /locus_tag="LP452_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2014287.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LP452_001558"
     CDS             9227..9553
                     /locus_tag="LP452_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2014288.1"
                     /translation="MISSDGIRGYNDAIILSIINKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LP452_001559"
     CDS             9554..10303
                     /locus_tag="LP452_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014289.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LP452_001560"
     CDS             complement(10383..10838)
                     /locus_tag="LP452_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL2014290.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LP452_001561"
     CDS             complement(11265..11540)
                     /locus_tag="LP452_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014291.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LP452_001562"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LP452_001562"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL2014292.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP452_001563"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP452_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL2014293.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LP452_001564"
     CDS             14770..17436
                     /locus_tag="LP452_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2014294.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LP452_001565"
     CDS             17800..18552
                     /locus_tag="LP452_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2014295.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LP452_001566"
     CDS             complement(18574..19728)
                     /locus_tag="LP452_001566"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL2014296.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..21148
                     /locus_tag="LP452_001567"
     CDS             19913..21148
                     /locus_tag="LP452_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBL2014297.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKSFISLRHLNGAVN"
     gene            21148..22353
                     /locus_tag="LP452_001568"
     CDS             21148..22353
                     /locus_tag="LP452_001568"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL2014298.1"
                     /translation="MEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIPMGIAQN
                     FQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKSEYQAVI
                     NAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDVKDAMGA
                     NMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNKEIGEQI
                     AKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAARNGLYQ
                     GLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQVIAAVG
                     LAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKMNQQTAI
                     QILEKIREK"
     gene            complement(22683..23387)
                     /locus_tag="LP452_001569"
     CDS             complement(22683..23387)
                     /locus_tag="LP452_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014299.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23574..23819)
                     /locus_tag="LP452_001570"
     CDS             complement(23574..23819)
                     /locus_tag="LP452_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014300.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23836..24687)
                     /locus_tag="LP452_001571"
     CDS             complement(23836..24687)
                     /locus_tag="LP452_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2014301.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24853..25857
                     /locus_tag="LP452_001572"
     CDS             24853..25857
                     /locus_tag="LP452_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL2014302.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26098..26454)
                     /locus_tag="LP452_001573"
     CDS             complement(26098..26454)
                     /locus_tag="LP452_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2014303.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26822..28009
                     /gene="tuf"
                     /locus_tag="LP452_001574"
     CDS             26822..28009
                     /gene="tuf"
                     /locus_tag="LP452_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL2014304.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         8975 a         4940 c         5804 g         8407 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc attaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaagtt ttatcagctt
    21121 acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg
    21181 ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa
    21241 gtgagtgaag tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa
    21301 aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca
    21361 aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt
    21421 ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag
    21481 ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa
    21541 gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta
    21601 gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca
    21661 aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt
    21721 gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac
    21781 aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt
    21841 gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg
    21901 gcagctacag gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga
    21961 aatggattat accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa
    22021 ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca
    22081 aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct
    22141 gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa
    22201 aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag
    22261 gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct
    22321 atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag
    22381 caagatttac agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg
    22441 tttattatta gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa
    22501 gatatcttca ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga
    22561 caggtcaagc tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa
    22621 attattatat gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat
    22681 ttttatcgat cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat
    22741 ttgactttgt atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata
    22801 gaaatagttg ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt
    22861 tcattttgtt ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa
    22921 cttaaagcgg cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt
    22981 tccgtttttt cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc
    23041 gcagttggtt catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga
    23101 agattgtcac ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca
    23161 gtattgaagt tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct
    23221 aatccgatcg ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca
    23281 taacgtcgca agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat
    23341 gaaggacgga attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt
    23401 cgctcatacc ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa
    23461 aacttgcgta tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga
    23521 aaaataaaaa atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt
    23581 ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag
    23641 ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt
    23701 gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg
    23761 gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata
    23821 aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc
    23881 atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt
    23941 attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag
    24001 cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat
    24061 ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc
    24121 atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg
    24181 attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa
    24241 attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca
    24301 tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat
    24361 caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat
    24421 cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct
    24481 aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc
    24541 agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc
    24601 cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag
    24661 acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata
    24721 agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt
    24781 taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa
    24841 gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa
    24901 actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat
    24961 tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca
    25021 gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt
    25081 gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca
    25141 gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc
    25201 aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc
    25261 ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct
    25321 gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa
    25381 aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa
    25441 aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa
    25501 tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg
    25561 ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct
    25621 tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta
    25681 caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa
    25741 ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg
    25801 gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa
    25861 tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa
    25921 ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt
    25981 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg
    26041 gagttaaacg cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta
    26101 attatcgatt ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct
    26161 taatacacct ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg
    26221 ttctctttct tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg
    26281 cttgtaggta taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc
    26341 attcgtcgtg atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa
    26401 acttccgccg agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt
    26461 cacctctttc cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac
    26521 agctgaattt taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact
    26581 atcttgaagt ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt
    26641 ataagacaga cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt
    26701 tcgaatctta caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat
    26761 aataaaaagg tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat
    26821 tatggcaaaa gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca
    26881 cgttgaccat gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg
    26941 cctagcaaat cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg
    27001 gattacgatc aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat
    27061 tgatgcacca ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga
    27121 tggtgcaatc ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat
    27181 tttattgtca cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt
    27241 agtcgatgat gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga
    27301 atatggtttc ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca
    27361 aggcgatcca gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat
    27421 cccaacacca gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc
    27481 aattacaggt cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg
    27541 tgatgaggta gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt
    27601 agaaatgttc cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt
    27661 acgggggatc caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat
    27721 tactccacat acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg
    27781 tcatactcca ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac
    27841 aggaacaatc acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat
    27901 tgatgttgac ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga
    27961 aggcggacgg acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact
    28021 cgaatatttt ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt
    28081 ttggaccgct ctttgttctt caattgtata agaagagatt gtagaa
//
LOCUS       DAFELO010000034        27633 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFELO010000034
VERSION     DAFELO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.034"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            119..766
                     /locus_tag="LP452_001575"
     CDS             119..766
                     /locus_tag="LP452_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL2014305.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            850..1794
                     /locus_tag="LP452_001576"
     CDS             850..1794
                     /locus_tag="LP452_001576"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL2014306.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1929..3380
                     /locus_tag="LP452_001577"
     CDS             1929..3380
                     /locus_tag="LP452_001577"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL2014307.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3401..4237
                     /locus_tag="LP452_001578"
     CDS             3401..4237
                     /locus_tag="LP452_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL2014308.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4237..4896
                     /locus_tag="LP452_001579"
     CDS             4237..4896
                     /locus_tag="LP452_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2014309.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5022..5711
                     /locus_tag="LP452_001580"
     CDS             5022..5711
                     /locus_tag="LP452_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014310.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5717..6673
                     /locus_tag="LP452_001581"
     CDS             5717..6673
                     /locus_tag="LP452_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL2014311.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6717..7229
                     /locus_tag="LP452_001582"
     CDS             6717..7229
                     /locus_tag="LP452_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014312.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7485..8033)
                     /locus_tag="LP452_001583"
     CDS             complement(7485..8033)
                     /locus_tag="LP452_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2014313.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8122..9303)
                     /locus_tag="LP452_001584"
     CDS             complement(8122..9303)
                     /locus_tag="LP452_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL2014314.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9539..11047)
                     /locus_tag="LP452_001585"
     CDS             complement(9539..11047)
                     /locus_tag="LP452_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL2014315.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11206..11397
                     /locus_tag="LP452_001586"
     CDS             11206..11397
                     /locus_tag="LP452_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014316.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11600..12517
                     /locus_tag="LP452_001587"
     CDS             11600..12517
                     /locus_tag="LP452_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL2014317.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12510..13583
                     /locus_tag="LP452_001588"
     CDS             12510..13583
                     /locus_tag="LP452_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014318.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13731..14249
                     /locus_tag="LP452_001589"
     CDS             13731..14249
                     /locus_tag="LP452_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014319.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14230..14622
                     /locus_tag="LP452_001590"
     CDS             14230..14622
                     /locus_tag="LP452_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL2014320.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14698..15633
                     /locus_tag="LP452_001591"
     CDS             14698..15633
                     /locus_tag="LP452_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL2014321.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15766..16545
                     /locus_tag="LP452_001592"
     CDS             15766..16545
                     /locus_tag="LP452_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2014322.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16538..16855
                     /locus_tag="LP452_001593"
     CDS             16538..16855
                     /locus_tag="LP452_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2014323.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17099..18709
                     /locus_tag="LP452_001594"
     CDS             17099..18709
                     /locus_tag="LP452_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2014324.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18866..19714
                     /locus_tag="LP452_001595"
     CDS             18866..19714
                     /locus_tag="LP452_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2014325.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19945..20424
                     /locus_tag="LP452_001596"
     CDS             19945..20424
                     /locus_tag="LP452_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014326.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20457..22685
                     /locus_tag="LP452_001597"
     CDS             20457..22685
                     /locus_tag="LP452_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2014327.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22814..24457
                     /locus_tag="LP452_001598"
     CDS             22814..24457
                     /locus_tag="LP452_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL2014328.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKINK"
     gene            complement(24530..25075)
                     /locus_tag="LP452_001599"
     CDS             complement(24530..25075)
                     /locus_tag="LP452_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL2014329.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25098..25583)
                     /locus_tag="LP452_001600"
     CDS             complement(25098..25583)
                     /locus_tag="LP452_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2014330.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25573..26385)
                     /locus_tag="LP452_001601"
     CDS             complement(25573..26385)
                     /locus_tag="LP452_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL2014331.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26524..>27633)
                     /locus_tag="LP452_001602"
     CDS             complement(26524..>27633)
                     /locus_tag="LP452_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL2014332.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9048 a         5026 c         5526 g         8033 t
ORIGIN      
        1 tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa
       61 ttttttcttc gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat
      121 gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc
      181 aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca
      241 ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag
      301 tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca
      361 agataaatac ccagatactg caattactag tttggaatta gatagtgatt ggggatctta
      421 cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca
      481 aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca
      541 aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa
      601 aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt
      661 aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat
      721 caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa
      781 aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga
      841 gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg
      901 aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa
      961 gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca
     1021 cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt
     1081 tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg
     1141 ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata
     1201 gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga
     1261 aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc
     1321 atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat
     1381 cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg
     1441 ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg
     1501 ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt
     1561 tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa
     1621 gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat
     1681 aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa
     1741 aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga
     1801 gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct
     1861 gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg
     1921 aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt
     1981 ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg
     2041 cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag
     2101 aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg
     2161 gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta
     2221 attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag
     2281 agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga
     2341 accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa
     2401 tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt
     2461 gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga
     2521 gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg
     2581 ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg
     2641 agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga
     2701 tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg
     2761 gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca
     2821 cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag
     2881 gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat
     2941 atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag
     3001 caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat
     3061 ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac
     3121 acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga
     3181 gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct
     3241 tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag
     3301 gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt
     3361 taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt
     3421 tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca
     3481 ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg
     3541 cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga
     3601 tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt
     3661 ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg
     3721 tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga
     3781 cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg
     3841 gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc
     3901 taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg
     3961 tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc
     4021 agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt
     4081 attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa
     4141 agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa
     4201 accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt
     4261 tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt
     4321 ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt
     4381 ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt
     4441 cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta
     4501 acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga
     4561 ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct
     4621 ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca
     4681 ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg
     4741 ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc
     4801 tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag
     4861 tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg
     4921 ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc
     4981 atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca
     5041 tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag
     5101 aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg
     5161 atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta
     5221 cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg
     5281 aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg
     5341 ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag
     5401 agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag
     5461 taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca
     5521 aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac
     5581 ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc
     5641 ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc
     5701 cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc
     5761 attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc
     5821 agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg
     5881 aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca
     5941 cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa
     6001 tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc
     6061 aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga
     6121 tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca
     6181 aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta
     6241 tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg
     6301 ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc
     6361 ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca
     6421 agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca
     6481 tcaactcttg attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt
     6541 ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca
     6601 cctgctagat catccaaaag attggcttaa atattattca aaagttcagg aactatatga
     6661 agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt
     6721 atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag
     6781 cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag
     6841 aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa
     6901 ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa atagatggta
     6961 gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta
     7021 ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca
     7081 aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca
     7141 tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa
     7201 gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca
     7261 ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag
     7321 cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg
     7381 tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt
     7441 tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat caataatcct
     7501 ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag
     7561 gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct
     7621 gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag
     7681 atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca
     7741 tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta
     7801 ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc
     7861 atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt
     7921 cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg
     7981 agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt
     8041 tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa
     8101 gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac ttaaaaagct
     8161 gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc
     8221 ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga
     8281 aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc
     8341 taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc
     8401 atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc
     8461 taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat
     8521 caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag
     8581 aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc
     8641 atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa
     8701 taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc
     8761 gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc
     8821 agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc
     8881 agaagctact ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag
     8941 accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc
     9001 agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc
     9061 tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat
     9121 ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag
     9181 aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact
     9241 gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact
     9301 caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact
     9361 aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa
     9421 aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa
     9481 aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct
     9541 attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt
     9601 ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct
     9661 caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg
     9721 ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg
     9781 atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga
     9841 tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac
     9901 ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat
     9961 cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt
    10021 ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt
    10081 cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt
    10141 cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca
    10201 cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt tcaataatac
    10261 gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg
    10321 catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat
    10381 aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg
    10441 agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag gaagtagcga
    10501 gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat
    10561 actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt
    10621 cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt
    10681 tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac
    10741 cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac
    10801 ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt
    10861 tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg
    10921 catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca tagtggttca
    10981 ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt
    11041 ttttcactag gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc
    11101 aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag
    11161 atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag
    11221 aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg
    11281 cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta
    11341 actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa
    11401 ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga
    11461 aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt
    11521 tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga
    11581 gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga
    11641 actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat
    11701 caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa
    11761 agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga
    11821 agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat
    11881 ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac
    11941 gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc
    12001 aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca
    12061 accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc
    12121 tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga
    12181 tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt
    12241 agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac
    12301 gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct
    12361 tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga
    12421 gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa
    12481 taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa
    12541 aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa
    12601 gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa
    12661 aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa
    12721 ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga
    12781 ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag
    12841 attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca
    12901 cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg
    12961 atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga
    13021 tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg
    13081 atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa
    13141 tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat
    13201 tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa
    13261 gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga
    13321 atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa
    13381 tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag
    13441 ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct
    13501 catctggaga ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc
    13561 cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat
    13621 tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg
    13681 tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa
    13741 ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc
    13801 aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa
    13861 gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg
    13921 gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt
    13981 caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt
    14041 ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt
    14101 tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag
    14161 gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag
    14221 gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt
    14281 ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa
    14341 acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa
    14401 gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa
    14461 gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc
    14521 tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg
    14581 aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt
    14641 aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg
    14701 gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta
    14761 gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa
    14821 agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa
    14881 gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc
    14941 agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct
    15001 tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata
    15061 ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa
    15121 gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa
    15181 atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca
    15241 ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg
    15301 atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc
    15361 atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct
    15421 gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt
    15481 gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat
    15541 attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca
    15601 gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa
    15661 atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta
    15721 caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa
    15781 agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca
    15841 gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga
    15901 ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg
    15961 acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt acgctataca
    16021 ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc
    16081 ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac
    16141 ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc
    16201 ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc
    16261 cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta
    16321 ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa
    16381 aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag
    16441 aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag
    16501 ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact
    16561 tttagttgtt tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt
    16621 tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt
    16681 atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg
    16741 gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat
    16801 ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt
    16861 tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg
    16921 catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt
    16981 ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga
    17041 ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat
    17101 gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt
    17161 tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat
    17221 gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg
    17281 tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga
    17341 taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat
    17401 cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc
    17461 taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc
    17521 aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac
    17581 ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt
    17641 gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt
    17701 cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt
    17761 cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc
    17821 tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt
    17881 tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc
    17941 agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt
    18001 gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat
    18061 gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt
    18121 attgattctt cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt
    18181 gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt
    18241 gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt
    18301 tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt
    18361 aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt
    18421 tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag
    18481 caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt
    18541 tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg
    18601 tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg
    18661 gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc
    18721 gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt
    18781 ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat
    18841 tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata
    18901 tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat
    18961 ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt
    19021 aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt
    19081 atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg
    19141 gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt
    19201 cattccttat gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt
    19261 agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg
    19321 agataatacc tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg
    19381 tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt
    19441 ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg
    19501 agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt
    19561 gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata
    19621 tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga
    19681 aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa
    19741 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
    19801 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
    19861 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
    19921 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
    19981 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa
    20041 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
    20101 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
    20161 attttagatt tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt
    20221 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
    20281 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
    20341 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
    20401 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
    20461 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
    20521 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
    20581 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
    20641 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
    20701 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
    20761 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
    20821 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
    20881 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
    20941 attacccttc tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc
    21001 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
    21061 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
    21121 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
    21181 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
    21241 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
    21301 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
    21361 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
    21421 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
    21481 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
    21541 agcatacaaa aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
    21601 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
    21661 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
    21721 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
    21781 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
    21841 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
    21901 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
    21961 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
    22021 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
    22081 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
    22141 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
    22201 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
    22261 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
    22321 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
    22381 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
    22441 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
    22501 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
    22561 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
    22621 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
    22681 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
    22741 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
    22801 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
    22861 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
    22921 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
    22981 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
    23041 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
    23101 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
    23161 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
    23221 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
    23281 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
    23341 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
    23401 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
    23461 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
    23521 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
    23581 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
    23641 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact
    23701 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
    23761 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
    23821 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
    23881 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
    23941 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
    24001 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
    24061 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
    24121 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
    24181 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
    24241 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
    24301 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
    24361 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
    24421 accttatgaa gctcggttat ataaaataaa caagtaaaac ttcctcgtta aagttcatta
    24481 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
    24541 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
    24601 gggttttcaa acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg
    24661 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
    24721 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
    24781 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
    24841 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
    24901 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
    24961 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
    25021 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
    25081 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
    25141 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
    25201 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
    25261 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
    25321 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
    25381 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
    25441 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
    25501 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
    25561 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
    25621 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
    25681 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
    25741 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
    25801 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
    25861 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
    25921 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
    25981 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
    26041 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
    26101 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
    26161 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
    26221 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
    26281 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
    26341 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
    26401 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
    26461 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
    26521 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
    26581 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
    26641 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
    26701 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
    26761 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
    26821 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
    26881 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
    26941 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
    27001 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
    27061 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
    27121 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
    27181 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
    27241 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
    27301 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
    27361 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
    27421 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
    27481 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
    27541 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
    27601 caaagcaggt tgttccatta aataaagaat agg
//
LOCUS       DAFELO010000035        27590 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFELO010000035
VERSION     DAFELO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.035"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            476..1339
                     /locus_tag="LP452_001603"
     CDS             476..1339
                     /locus_tag="LP452_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2014333.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LP452_001604"
     CDS             complement(1468..3090)
                     /locus_tag="LP452_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2014334.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LP452_001605"
     CDS             3339..3860
                     /locus_tag="LP452_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL2014335.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LP452_001606"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LP452_001606"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL2014336.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LP452_001607"
     CDS             5941..6759
                     /locus_tag="LP452_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL2014337.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LP452_001608"
     CDS             complement(6862..7059)
                     /locus_tag="LP452_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014338.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LP452_001609"
     CDS             7228..7806
                     /locus_tag="LP452_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL2014339.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LP452_001610"
     CDS             7901..8038
                     /locus_tag="LP452_001610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014340.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="LP452_001611"
     CDS             complement(8851..9354)
                     /locus_tag="LP452_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2014341.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="LP452_001612"
     CDS             9713..10738
                     /locus_tag="LP452_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2014342.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="LP452_001613"
     CDS             11085..11963
                     /locus_tag="LP452_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL2014343.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="LP452_001614"
     CDS             12439..12555
                     /locus_tag="LP452_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL2014344.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..13821
                     /locus_tag="LP452_001615"
     CDS             13483..13821
                     /locus_tag="LP452_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014345.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13915..14145
                     /locus_tag="LP452_001616"
     CDS             13915..14145
                     /locus_tag="LP452_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014346.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14545..14916
                     /locus_tag="LP452_001617"
     CDS             14545..14916
                     /locus_tag="LP452_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL2014347.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15190..15882)
                     /locus_tag="LP452_001618"
     CDS             complement(15190..15882)
                     /locus_tag="LP452_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2014348.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15885..16670)
                     /locus_tag="LP452_001619"
     CDS             complement(15885..16670)
                     /locus_tag="LP452_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2014349.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16907..18307
                     /gene="lacG"
                     /locus_tag="LP452_001620"
     CDS             16907..18307
                     /gene="lacG"
                     /locus_tag="LP452_001620"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL2014350.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18332..18649
                     /locus_tag="LP452_001621"
     CDS             18332..18649
                     /locus_tag="LP452_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2014351.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18662..20335
                     /locus_tag="LP452_001622"
     CDS             18662..20335
                     /locus_tag="LP452_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL2014352.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20349..21980
                     /locus_tag="LP452_001623"
     CDS             20349..21980
                     /locus_tag="LP452_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL2014353.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22852..24042
                     /locus_tag="LP452_001624"
     CDS             22852..24042
                     /locus_tag="LP452_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBL2014354.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24176..26365
                     /locus_tag="LP452_001625"
     CDS             24176..26365
                     /locus_tag="LP452_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2014355.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26362..>27590
                     /locus_tag="LP452_001626"
     CDS             26362..>27590
                     /locus_tag="LP452_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2014356.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         8847 a         4566 c         5443 g         8734 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13861 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    13921 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    13981 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14041 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14101 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14161 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14221 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14281 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14341 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14401 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14461 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14521 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14581 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14641 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14701 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14761 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14821 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    14881 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    14941 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15001 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15061 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15121 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15181 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15241 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15301 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15361 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15421 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15481 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15541 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15601 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15661 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15721 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15781 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15841 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    15901 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    15961 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16021 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16081 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16141 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16201 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16261 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16321 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16381 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16441 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16501 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16561 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16621 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16681 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16741 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16801 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16861 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    16921 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    16981 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17041 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17101 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17161 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17221 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17281 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17341 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17401 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17461 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17521 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17581 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17641 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17701 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17761 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17821 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    17881 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    17941 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18001 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18061 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18121 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18181 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18241 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18301 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18361 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18421 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18481 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18541 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18601 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18661 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18721 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18781 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18841 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    18901 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    18961 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19021 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19081 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19141 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19201 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19261 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19321 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19381 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19441 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19501 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19561 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19621 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19681 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19741 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19801 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19861 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    19921 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    19981 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20041 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20101 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20161 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20221 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20281 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20341 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20401 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20461 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20521 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20581 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20641 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20701 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20761 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20821 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    20881 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    20941 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21001 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21061 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21121 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21181 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21241 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21301 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21361 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21421 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21481 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21541 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21601 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21661 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21721 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21781 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21841 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    21901 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    21961 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22021 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22081 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22141 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22201 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22261 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22321 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22381 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22441 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    22501 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    22561 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    22621 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    22681 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    22741 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    22801 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    22861 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    22921 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    22981 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    23041 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    23101 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    23161 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    23221 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    23281 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    23341 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    23401 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    23461 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    23521 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    23581 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    23641 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    23701 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    23761 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    23821 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    23881 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    23941 tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa
    24001 ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg
    24061 aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt actaacataa
    24121 tttatgataa acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt
    24181 tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga
    24241 taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc
    24301 attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga
    24361 tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt
    24421 tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc
    24481 tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat
    24541 ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga
    24601 attagactct catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt
    24661 ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt
    24721 aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg
    24781 ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct
    24841 tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac
    24901 aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt
    24961 aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca
    25021 actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc
    25081 agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt
    25141 agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt
    25201 tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca
    25261 tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac
    25321 tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat
    25381 gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa
    25441 ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag
    25501 atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg
    25561 agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga
    25621 cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt
    25681 gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc
    25741 aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa
    25801 agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac
    25861 aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc
    25921 aattatagat gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca
    25981 aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa
    26041 ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta
    26101 tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa
    26161 aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc
    26221 tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt tattagagta
    26281 ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat
    26341 actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat
    26401 ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg
    26461 agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga
    26521 tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa
    26581 cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac aatacatcag
    26641 ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga
    26701 tttacaatta ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg
    26761 aatttaaatc tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt
    26821 aaaattaatg aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa
    26881 aataaagaaa ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac
    26941 agtaacgttg cacgtaatat tgctagtaat ataagggcga tttatgtgga tgaatttcaa
    27001 gatactagag aattacaata caatgtaata gctaaattac ttcaaaacaa ccctcaaatt
    27061 cagtcaatgt ttgttggaga tattgaccaa gcaatttatg gatcattaga tggtatagct
    27121 aaatcagttg aagaactaga aatgctaact agtcacactt ttcaatcaaa gacgctacat
    27181 ggctgttaca ggtctaatca gagattagta gacttctact ctaattttca gagttgtcct
    27241 tacgagattg aaagcagagg gaataataaa aacgaccgtg gttttataag ttatgattgc
    27301 aaagttacta aggaggattt acccaacata ataaaaaata taataaagga aaagattgaa
    27361 agcggcattc atgaaaaaga tatttgtgta cttgctcctc aacactatcc tctgttttca
    27421 ctgggtgagt atttaaagaa agaattaccg tattgcaatt ttgatgctat aaatatatca
    27481 ccaattaagg taaataatca tagtttattt ttcaagttag caattttgta ctttactgaa
    27541 tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg atattttaat
//
LOCUS       DAFELO010000036        27415 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFELO010000036
VERSION     DAFELO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.036"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(106..1293)
                     /locus_tag="LP452_001627"
     CDS             complement(106..1293)
                     /locus_tag="LP452_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2014357.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            1615..2457
                     /locus_tag="LP452_001628"
     CDS             1615..2457
                     /locus_tag="LP452_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2014358.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            2457..3404
                     /locus_tag="LP452_001629"
     CDS             2457..3404
                     /locus_tag="LP452_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL2014359.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            3419..3652
                     /locus_tag="LP452_001630"
     CDS             3419..3652
                     /locus_tag="LP452_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL2014360.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            3983..4567
                     /locus_tag="LP452_001631"
     CDS             3983..4567
                     /locus_tag="LP452_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2014361.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            4698..5365
                     /locus_tag="LP452_001632"
                     /pseudo
     CDS             4698..5365
                     /locus_tag="LP452_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            5376..6791
                     /locus_tag="LP452_001633"
     CDS             5376..6791
                     /locus_tag="LP452_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2014362.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYTNMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            6809..7378
                     /locus_tag="LP452_001634"
     CDS             6809..7378
                     /locus_tag="LP452_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL2014363.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            7488..9242
                     /locus_tag="LP452_001635"
     CDS             7488..9242
                     /locus_tag="LP452_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014364.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            9202..10968
                     /locus_tag="LP452_001636"
     CDS             9202..10968
                     /locus_tag="LP452_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014365.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            11491..11760
                     /gene="rpsN"
                     /locus_tag="LP452_001637"
     CDS             11491..11760
                     /gene="rpsN"
                     /locus_tag="LP452_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL2014366.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            11775..11954
                     /gene="rpmF"
                     /locus_tag="LP452_001638"
     CDS             11775..11954
                     /gene="rpmF"
                     /locus_tag="LP452_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL2014367.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            11986..12363
                     /locus_tag="LP452_001639"
     CDS             11986..12363
                     /locus_tag="LP452_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014368.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            12384..12533
                     /gene="rpmG"
                     /locus_tag="LP452_001640"
     CDS             12384..12533
                     /gene="rpmG"
                     /locus_tag="LP452_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2014369.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            12557..13540
                     /locus_tag="LP452_001641"
     CDS             12557..13540
                     /locus_tag="LP452_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL2014370.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            13570..13692
                     /locus_tag="LP452_001642"
     CDS             13570..13692
                     /locus_tag="LP452_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL2014371.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            13710..15233
                     /locus_tag="LP452_001643"
     CDS             13710..15233
                     /locus_tag="LP452_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL2014372.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            15257..15496
                     /locus_tag="LP452_001644"
     CDS             15257..15496
                     /locus_tag="LP452_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014373.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(15689..16150)
                     /locus_tag="LP452_001645"
     CDS             complement(15689..16150)
                     /locus_tag="LP452_001645"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2014374.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(16345..16563)
                     /locus_tag="LP452_001646"
     CDS             complement(16345..16563)
                     /locus_tag="LP452_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014375.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            16681..18297
                     /locus_tag="LP452_001647"
     CDS             16681..18297
                     /locus_tag="LP452_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL2014376.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(18538..19878)
                     /locus_tag="LP452_001648"
                     /pseudo
     CDS             complement(18538..19878)
                     /locus_tag="LP452_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(20154..20309)
                     /locus_tag="LP452_001649"
     CDS             complement(20154..20309)
                     /locus_tag="LP452_001649"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL2014377.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(20501..20695)
                     /locus_tag="LP452_001650"
     CDS             complement(20501..20695)
                     /locus_tag="LP452_001650"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2014378.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(20605..20937)
                     /locus_tag="LP452_001651"
     CDS             complement(20605..20937)
                     /locus_tag="LP452_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2014379.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(21451..21642)
                     /locus_tag="LP452_001652"
     CDS             complement(21451..21642)
                     /locus_tag="LP452_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014380.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(21736..22122)
                     /locus_tag="LP452_001653"
     CDS             complement(21736..22122)
                     /locus_tag="LP452_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2014381.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            22816..24021
                     /locus_tag="LP452_001654"
     CDS             22816..24021
                     /locus_tag="LP452_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL2014382.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            24038..24472
                     /locus_tag="LP452_001655"
     CDS             24038..24472
                     /locus_tag="LP452_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014383.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            25089..>27415
                     /gene="esp"
                     /locus_tag="LP452_001656"
     CDS             25089..>27415
                     /gene="esp"
                     /locus_tag="LP452_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL2014384.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         9408 a         4021 c         5131 g         8855 t
ORIGIN      
        1 caagtaaaaa ttagccaaat caaaaaatat tttataacag ataattccga aaaaagggta
       61 caacaaataa gcttcttacc attagttttt ttgaatgttc aatagctaat cagaaacaat
      121 tatataaggc tcgtttttag tcaaaatagc atgaatcacg taacaaagct tacgagaaac
      181 cgcacctaag gcagttagat gatgttttcc ttcggaaact tttttctggt aaaacgcact
      241 aaaaacagga tcacattgag aagctactaa agccgccgaa aacaaggctt ttcgcaaata
      301 tggagaccct cgtttactca taacattatg cgttgcctca tattgccctg attgtgtcac
      361 ggaagcgtct atcccagcgt aagcaacgag ttttttcggg ttagaaaatt tttgaatatc
      421 tccgatctca ccaagaatag tcgcaccgtt tataggacca attccaggaa ttgtgagaat
      481 aacagaattt aaagtgtcca ttatgtgctt gatttctctt tccgtttcct ttatttggct
      541 ttcaatgaat tttaattgtt caatcattga acgtagttgg aaggtgaacg cttcttgagc
      601 aaattttact ccaaaggagt tagaagctgc ttgcttcaat tggtccgctt tttctgcctt
      661 gattcggttc ctgcttaact cagctaagta ttgggccaaa gtttctgagg aaatgttttc
      721 taagtcgatc ggggcactaa acgccagcaa taactcttta gaagcttttc cgaaaacgcc
      781 ctgcttggta aaaatcgtcg cgtattctgg aaagacttga tccaatacgg ctataatttt
      841 tcttttgaag tcgctcgctg tacctactaa ataagttcgg taacgagaaa gttgtttcaa
      901 agaaaaaaca ttttcatcgg ataagaccgt ttcgacaaat gagccatagc gcattaagtc
      961 agcaatcaaa acagaatcaa taatatcgtt ttttcgtttc cgtatttctg tacctttacg
     1021 ccatccgtct gtttgaaggg gattcacaac gtgaatcaaa taatctgatt cgtgaaggta
     1081 tgaaaagatg gacagccaat agtgccccgt ggcttccatc cctataacaa agtcactggg
     1141 tgctccggaa tagtcgacca ttcgctcaat taatgactct gctccttcag tggtgttagg
     1201 aaatgaaaaa ccttttagta atacctttcc ttcctcgttc atgatagaag caacgtgagt
     1261 gcgtttaccg atatcaatgc ctaaaaagta catgtatatc tgacctttca aaaaaagaat
     1321 tttggattga aatccactca actgaaaagc gtcactacct tgtacgagat acggagtaaa
     1381 gtaaggactt tcaacatcta actcatacgc aatccactta tcagagagag gagccagtct
     1441 ctcgagtgcg ggttcaatgg cccaaggggg gaacggctta gcctctcaat ccaataaata
     1501 tattatctca aaagaaatga gttaatacca aattaaaata acgatggttc atagggttat
     1561 ccaatcaaaa ccggaaggtt aaaaccctaa ctctaatata caagggggaa taatatgctt
     1621 caaaatttta ttaatggtct gtatgacttt cactttttac agaatgctct aattacatct
     1681 gtagttatag ggattgtttc tggaacagta ggttgtttta ttattttaag aggaatgtct
     1741 cttatgggtg atgctatttc tcatgctgtt ttgccaggag tagcaatttc ttatatttta
     1801 ggtattaatt tttttattgg tgcgattgta tttggtctat tagcttctat cattattact
     1861 tttataaaaa gcaatagtgt aataaaagga gatacagcaa ttggtattac tttcagttct
     1921 tttttagcat taggagttat ccttatagga gttgcaaata gctcaacgga tttatttcat
     1981 atcttatttg gaaatatttt agctgtacaa gatattgata agtggattac aattggcgtt
     2041 tctgttttag tgttggttac agttgttcta ttttttaaag aattattaat cacttctttt
     2101 gatccattaa tggcaaaagc aatggggatg aatgtcagtt tttatcatta tctattaatg
     2161 attctattaa ctttagtagc agtaacagct atgcaaagtg tgggaactat attaattgtt
     2221 gcaatgttaa ttacaccagc tgctacagca tatttatatg caaacagctt aaaaactatg
     2281 atatttattt cggcgacagt cggagcgtgc tcatcattgc taggtttgtt cattggttat
     2341 agttttaaca tagcagcagg atcaagtatt gttttaacag cggcattctt atttgtaatt
     2401 agttttctaa tctcaccaaa acaaaaattt ttaagaaaga aggagagaaa gttatgatga
     2461 gaaaatggaa agcagtactg ggaagtctgg ggattttgat tgctcttttt atattcgggg
     2521 catgttcaac aaatagtaaa gacaaagata cagtggcttc gaacgaaaaa ttaaaggtag
     2581 tagttactaa ttcgatttta gcagatatta ctgaaaatat agcaaaagat aaaattgatt
     2641 tacacagtat cgtacctatt gggaaagatc cccacgaata tgaacctttg cctgaagatg
     2701 ttcaaaaaac ttcaaaagca gatttgattt tttataacgg tgttaacttg gaaactggag
     2761 gaaatgcttg gtttacaaaa ttagtaaaaa atgcgaacaa agaggaaaac aaagactatt
     2821 ttgcagcaag tgatggcata gatgttattt acttagaggg tcagagtgag aaagggaagg
     2881 aagatcccca tgcttggtta aatttagaaa acggtattat ttacgctaaa aatattgaaa
     2941 aacagttagc ggaaaaagat cctgataata aaaaattcta taaagaaaat ctagataagt
     3001 atattgaaaa attggattct ctagacaaag aagctaaatc taaatttgct tcaattccga
     3061 atgataaaaa aatgattgtt acaagtgaag gatgctttaa atatttctcg aaagcgtata
     3121 atgtgccttc tgcttacatt tgggaaatca acactgaaga agaaggaaca ccagatcaaa
     3181 taaaacactt agttgaaaaa ttacgcacaa caaaagttcc ctccttattc gtagaaagca
     3241 gtgtggacga tagaccaatg aaaacagtat caaaagatac caatattcct atctattcaa
     3301 cgatttttac tgattcaatt gcagaaaaag gacaagatgg tgatagttac tatgcgatga
     3361 tgaaatggaa cctggataaa attgctgaag gcctttcgaa ataatttgga gggtaggtat
     3421 ggtacaaatt aaaacgacta ctatactaat ttatttacta gcaattcagg aattatcaaa
     3481 aagaaaaaag aaattaaaaa ataatgattt agcaaaagtt ctaagtgtta gtccagcatc
     3541 tgtaagtgag atgctggcta aacttagata tgaggactat ttagatatag gatttcaatt
     3601 aactgataaa gggaaaaaat ttattgacga ttacaaaaaa ggttttaagt aaaagacttt
     3661 atttttaggg aggataagaa aatggctatt ggagtgatta tgataggact taccctaata
     3721 gctggatata atttttttaa atctttctcg gattatttta gaaaaggtga atgatttaga
     3781 tgagtctatt aattcttgta ggattgcttt ttgtatcagt tattttgctc tttctcatta
     3841 gtttagctct aaggaatgag ttcagtaata gacgttaacg attgaaactt caagtcctgt
     3901 gtaagataat atagttaatt catcttatta ggaggaagta cccatagaaa ccagagatta
     3961 tattacaact caccaaaaat taatagaaag tggactaagc cattttgaaa aagaaggatt
     4021 tcagcaaact aagataaggc aactgtgctt gaccgcaggc gtaactacgg gtgcttttta
     4081 taaacatttt acttctaagg aagcgttatt tgaagaaatt attcagccgc atattaatgc
     4141 gcttattaat atgtataatg aagagtttac caaatacctc ttaaacttag aaaatataga
     4201 tatagagaaa agttggagag agaacttcaa tgaattagat ccatttatta attatatttt
     4261 tgagaataaa cgtatattcg atttggtttt attccaatca gctggaacca aatatcaaga
     4321 tattgttgaa aaaattacat catttgtgac aaaaaagaca gtctgttgtc tagataagtt
     4381 aaagactatc ggaatgatca atcaaaattt agtctttaat gaagaagaaa tccacttcta
     4441 catttactca ttctacgcta cattctatga tatattgaag catagctatt caaaagaaaa
     4501 gactatgcta ttaaccagaa gattgtatca attttctgtt cctgggtgga ttgaattact
     4561 gagttaatta aaaaaaagct tgcattgaga attgttctca gttatgcttt ttgataagat
     4621 cgcactgtta tctgaataac ggtgcatttt tgtcagctta agataacagt gcgatcttat
     4681 tttttggagg tgaaagagtg tcaaaaatca atccgttaac cttgattact ttaaatattt
     4741 ttttccggtc attatttttt taggaaaggg aaatatattt caagtggggt gtttcattat
     4801 tgctacagtt ttagtattga tgtttcgtaa aataaagaca tgtatttatt tcatcttgat
     4861 ttattcagtt ctgtttttag taatgaaaat tattgatgct atcaagatag agttttttac
     4921 tatatttttt gggacatcct tatatattgt atttcgaatg ttgccggtcg taatgatctc
     4981 atggttgttg gttagtgtct atactagcaa tgaattatta tcctctttag aaaaaataca
     5041 tttaccgaaa aaaataatgt taagtattac agtagtgatc agattctttc cgacttatcg
     5101 ttctgaaata agaatgatta aagaatcttt aaaaatgaga aacattcgct tgtctttttt
     5161 tcagcctctg caatatttgg aatttctgtt agttcccgtt cttatacgtg caactatgat
     5221 tgctgaagaa atgaccgcta atgcaataac aaaggggata gaatctcctg tgaaaagaag
     5281 ttcttactat aaaacaaaca tgagcttatt agactatatc atcctgtttt ccgttactat
     5341 ttttttctta agtattttga tttgaggtga aagaattgtt tgaatttaac aatgtttctt
     5401 tttcatataa ggatgttaag gaacaagcgc ttgttgacct taatttttca ataaaaaaag
     5461 gcagtcttac attgattact ggttcttctg ggagtgggaa atccacacta ttaaagttaa
     5521 tgaatggact tattccaaga ctttatgacg gaaaattggt tggggaaatt ttatttgaaa
     5581 ataactcgct acaatcctta acacaagaaa gtatatcaaa aaatattggc tacgtaagtc
     5641 aaaatcctag aggacaattc tttacaacaa atacaacgag tgagttagta ttttcgatgg
     5701 aaaactttgg atttacaaaa gagcatatgg ataaaagaat caatgaatta gctgaattat
     5761 tagatctttc aaatatatta gataaaaaca ttttttcgct atcaagtggt gaacggcaga
     5821 aaatttctat tggatgtagt ttatcgctaa actctaaatt aattatattg gatgaaccgt
     5881 cttcaaattt ggacttcaaa gcgactaaga aacttgctga cttactttgt gatttaaaaa
     5941 ataaaggatt tactattgtt atagctgaac atcgttttta ctatttgaaa gaattaattg
     6001 atcaagttct atttgttaaa aatagaactg taaaaactac aactattcag ttgttaaaaa
     6061 ctgaaaacaa taacgcatta aggaaatttg acatttttaa tagttcgatt gaagttccct
     6121 atatcgaaaa ggtaagtgca gatgtattaa aagttgaaga aattacttat gaaaatattc
     6181 taaaagacat aaattttgat gtcaaagttg gagatgttat tggtttggta gggaaaaacg
     6241 gtgtggggaa aacaacctta ctaagaatac tcatgaagat tattcgccct accaaaggaa
     6301 agataatcga aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa gaaatggatt
     6361 accaattttt cacagaatca gtgaggagcg agttagccct ggggaatgaa atagttagta
     6421 aagatcagca agaaaaaatt ttaaaaaaaa tggaacttta taaaatgaaa gatcaattac
     6481 cgtttgattt atccggtgga gaaaaacaaa gattattggt ttctatagct tcattgtcaa
     6541 aaactaattt atttctattt gatgaaccta caagtgggtt ggattatacc aatatggaaa
     6601 ggattgctga ccttattaaa gacttaaaag aaaagggtgc agtagttata gccactcatg
     6661 atccggaatt tctttataaa acatgcaata gaattattta tttaaaaaat ggtggaataa
     6721 aaaaagatat aaaacttaga gtagaagatt caactttgat agaacagatt tttaatgaca
     6781 taacgaaata gaattgagga gaatatatat gaaaacaaaa gattatattt ttattggttt
     6841 agctacagtt atttccctag taatttactt tttttcaata atggttagta gtattggtgg
     6901 ggcgtttggt cacagcatat ccccagggat ttttggatta ttatctggaa tcatatttgt
     6961 ttatattagc tataactacc ctagaaaagg aatttttact atttacacaa ttgttttatt
     7021 gttatttttt acgttaatgg gcggggcgta cttaccttgg tttatcagtt cgattagtat
     7081 ggcgatctta gctgatatta ttctatctgt atttggatat gatcgagcaa ttcctcaagt
     7141 tgtgtcttgg gttctaatgc aactaggcag tgctgccgga cagtggattc ccatatggtt
     7201 ctttacagat cgttttcgtc aagactggat agatagagga caatctgctg caacaatgga
     7261 tgcaatgatc cactacgcgg tgggtatatg gggaattatt tcagtgttag ttgtagcaag
     7321 tttatctatg attggtgtat tgattggaag aaaagtctta aaaaaataca aaaaatagaa
     7381 cgtagtttac cttctatctg tttgtacatc tctaatttac gctattacgg aaagcattaa
     7441 cagttgtaaa cgaataagag aagaaattga atgcggggga ggaaaaaatg aaagtataca
     7501 aaaaattatt tagttatgtc cctaaaatga aatactacgg atggttagcc actttatttt
     7561 ctgatttttc tgtcgtatta acagtctatg gatattacag tatttacaaa tttcttcagt
     7621 ctctaattat tgtaggagat gaatatttag ctaagagtta tgcggttcaa actgttgctt
     7681 ggttaacttt aggagcttta ttttatattt tttctggctt attttctcat atccttgggt
     7741 ttcgcttaga gactaatttg agaaaaaagg gaattagcgg tttaacagaa gcaagttttc
     7801 gtttttttga tttaaattct tcgggatata ttcgaaagac aattgatgac aacgcggcta
     7861 aaacacatat ggcaattgct cacttgattc cagatagtgc tcaagccata gtaacacctg
     7921 ttttatcagt tgccttaggc tttttcatta actttaaaat tggattggtt ctaatcgcca
     7981 tgttagtaat tggtgtattc ttgttaaaga aaatgacaga gaatacaaat tttattcaaa
     8041 aatatcaaga atcgttagat gtgttaagct ctgaaacggt agaatatatc cgcggaattc
     8101 aagttataaa gatatttggt gctaaagtaa catcatttaa agccttgaat aaagcaatta
     8161 acgattatgc taagtttgca tataagtatt ctttgagtgg gaaaacacct tttgttcttt
     8221 ttcaatggct attttttggc atagtttgca ttttgatagt tcctataacc ttttttataa
     8281 cttctcttac cactcccaag atcttagctg ttgacttgtt gatgttgttt tttttaagtg
     8341 gaattatttt tgtttctttc atgagaataa tgtatgtaag tatgcacttg tttgaagcaa
     8401 gatatgcagt cgaaacacta gactcccttt atttagaaat gaagcaagac caactaaaat
     8461 atgggaacga gacacagatt aaaaattgtg acattgcatt tgaaaatgtc agcttttcat
     8521 acaaagaaga gtgtgtttta gaaaatttca acttagtttt agaacaaaaa agaatttatg
     8581 cattagtagg aaaatcagga agcggaaaga gtacgatagc taaactaatt tcaggtttct
     8641 acaatgtgga taagggacag attaaaattg gtggtaagcc tttaaatcac tattcaaaag
     8701 acacaattat taacacaatt gcttttgtct ttcaagatac gaaactattc aatcagagta
     8761 tttacgataa cgtttctata gcaaatcctc aagcgccagc aagtaaagta tttgacgcgc
     8821 taagattagc cggttgcgaa tcgattttag aaaaatttcc cgaaagagag cataccttaa
     8881 ttggtagtaa aggagtttat ctgtcaggag gagaaaaaca acgtatagct attgccagag
     8941 caattttaaa agatgcaaag attgttatta tggatgaagc cagtgcatct attgatccag
     9001 ataatgagta tgaactacaa aaggcttttc aatatttgat gaaagaaaaa acagttatta
     9061 tgatagctca tcgattatcc agcattaaat ctgtagatga aattcttgtc ttgtcagaag
     9121 gaaaaatagt agaacgtgga aatcataatg aattaatggc taaacatcga gtgtataaac
     9181 aattggtcac taattatgag aatgcaaatg attggaggct tgacaatgaa aaatttttat
     9241 aaaaaaaaat ttgctttaac tgatcagggc gcagaagctt taactaaagc ttctatttct
     9301 agtttttttg tatactgcat taatatggta ccagcattca ttataatgat gctgatagac
     9361 gaattagttt tagaaaatgc aaagccccgt tggctttatt ttgcagtatc gtttgttact
     9421 ttgcttttca tgtattggtt gttagatagg gagtatgaaa acttatataa tagtacttat
     9481 aaagaaagtg cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt accattatcc
     9541 tatttttcaa aacataattt atcagattta tctcaaacta tcatgtctga cgttgaaggt
     9601 attgagcatg cgatgagtca tgcaatacct aaagccggcg gtatggctct gtttttccct
     9661 tttatttcag tgatgctttt ggttggtaat gtcaaaatgg gattagctgt tattttgcca
     9721 acgttattta gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa agccaatact
     9781 aaatattacg atactttgag agaaaactcg gaagaatttc aagaaactat tgaattgcag
     9841 caagagatta atagctttaa tctatctaaa aaagttcaag acagactttt caaaaaaatg
     9901 gaagagagtg aaaggattca tttaaaggta gaattaagta ctttttcagt catggcctta
     9961 tcctctattt tctcatatgt tagtttagca gtagtcattc tagtaggtgt tcacttactg
    10021 ttaacgggtg aagtgactat actctacgtc gttggttact tactagccgc aataaaaata
    10081 aaggattcct tcgattctat gaaagaagca gtgcttgaaa tattttattt agctcctaaa
    10141 atacagcgaa ttagagctat gaaggaaacc agtatccaag agggatcaga tagtcctctg
    10201 aaatcttttg acattgagtt gcgagatgtc tctttctcat atgataacaa taccccaatt
    10261 ttagatcata tctcttttac ggctaaacaa ggagaagtga ctgcgttagt aggtgctagt
    10321 ggttccggga aaactagtat cttgaaatta gtgtctagac tgtatgatta tgatgaaggc
    10381 tgtattttaa ttgatgggta tgatattaaa cgtgtatctc cagcttcttt gtttagcaaa
    10441 atcgctattg tttttcaaga ggtaactttg tttaatacaa gtattttgga aaatatacgt
    10501 attggaaata gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt agctaactgc
    10561 gaagacttta ttgaaaaact acctgacggt tatcatacat tggtcggaga aaacggaagt
    10621 tcactttctg gaggagagcg acaacgccta tccatagcac gggctttttt gaaaaatgcg
    10681 ccaattttaa ttttagatga aattactgcc agtttagatg cggaaaatga aaaacgtatt
    10741 caggaaagtt taaatcgttt aatacaagat aagacagtac taattatatc gcatcgcttg
    10801 aagtctattg aaaaggtaaa taaaattgta gtgatggatc aaggaaaact agttgatcaa
    10861 ggtactcata atgagttgta tcgaaggtca gaaatataca aaaatctcat aaagaaaact
    10921 aaactatctg agaaattcgt ctatgaaaaa gaagctcaat cacgatgatg aaataggctt
    10981 taaattaacc tacaattttt gggatatagt tataattctt tttcaaaaag aataatttta
    11041 ttaaacagca tctaattttg aatacacgaa agaatcagta tgattgaatt agttcactta
    11101 atgtctattc ttataatata aggaataatc aaagttttaa aaaattggcg ataaaccagt
    11161 taaacataaa agattgaata aaaagttata gtttctttca tattgccgtt ttaagtagag
    11221 gaaaagctac ttaaaacggt atttgtgttt gattggacta ataaaagtga attcttattt
    11281 ttcagagcct aaactgcata ttcttttaaa tgatttgatt atctctatgt accgataaat
    11341 tcgttttcaa atagttatct attcatatga gttttatttc atgttattta ttgttgacaa
    11401 gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat ttgaggtgtc
    11461 taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca
    11521 aagaaacaaa tggcgatgat tgaaaagtat gcagataaac gtcaggaatt aaaagccgca
    11581 ggagatcgca ccgctctcgc taaattgcca agagattcaa atccgaatcg tctgaggctt
    11641 cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa agttcggtat gtcccgtatt
    11701 aagtttagag aacttgccca tcaagggttg attcctggag taaaaaaagc aagttggtaa
    11761 aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa
    11821 gaagaactca tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt
    11881 atagacgtag ccatcatgta tctttaaaag ggtactataa tggaaagaaa gtaattaaag
    11941 gagcttcaaa atagtttaat atataacttt taggagggaa tcaaaatgga aattatttac
    12001 ccacctttag ttgaagaagg attaaagtat tatcttgaaa cgacccagca atcgctagat
    12061 aaatgcactt tttatcgttc gatggtagag agaggaatta ttactgaaac gggtttaccg
    12121 acccaacagg caattgaaaa tggattagtc aaagattact atgaggacca aggcttatca
    12181 tttgatgatt ttttaagaat ctatccaatt tttgaagagt atgacgagga gttatttcaa
    12241 tgtatcgatg gttattggga gatacctgtt gatatgaaag aaaatttagt ttcacaattg
    12301 gaatctggcg agttaacttt tgaagatgca caacaaattc aagcatatct tgaagatcga
    12361 taagtattga aggaggaaga tttatgcgtc aaaatattat tttggaatgt gtcgaaactg
    12421 gagaacgtct gtatctcacc agcaaaaata aacggaataa ccctgaacgg ttagaattaa
    12481 aaaagtattc gccaaaattg cgcagacggg caatttttaa agaagtgaaa tagctaagtg
    12541 gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt tctgggtttt taggggctgg
    12601 aaaaacaacg ctaatcaatc aagccttgca agtctcaccc taccccaggg aagaaattat
    12661 tataattgaa aatgagtttg gtgaagttgg gattgatcac aaattattag ttcatagcga
    12721 ggaacgggtg ttacaactaa ataatggatg tatgtgttgc agtcttcgca gtgatttatt
    12781 agcaatattg atttctttac tggaaacaat agaagaacat caacaaccaa tttcacaaat
    12841 catcattgaa actacaggta tagcagatcc ccaaccaatc attcaaacac ttttaacagc
    12901 gcctcaacta agaggaagag tatatatcga tagtttattg actgttattg atagtgacag
    12961 ttttgaactt attgaaagag aaccacaagc ccttaagcaa cttgtcatgg cagatcgcat
    13021 ttttgtgtct agaaaacaaa acttgaattt atctgcacta aaaaaattac agagagaaat
    13081 caaaagtatt aaccctttat ccgatattcg aatgttcaca catagtgaaa cgattcaaaa
    13141 aaaggatttt tataatttga aaaaatttaa tcatccctta gtttttgaaa gtgattcatc
    13201 agaaaatcac tataatggca aatactatca tcaacaccat caagtacatg agtttaaatc
    13261 aattctctta acttcagatc aagacataaa aaaagatttt ttactccaat ggattgactg
    13321 gctgatttta accaaccaag ggagcctgta tcgttttaaa ggattgattg ctttacaaga
    13381 gcaggatttt atagttgcca tgcaagaggt aaacgaacag gttaattttc aatacaccac
    13441 tcgacaaaaa gaccaaacca ctattattct aatcggaaaa atgttggatg aagaaaggat
    13501 caaacagagt tttgataaat tactggcgga aagcaaataa tgataagtag tgattggggg
    13561 taatttctaa tggagaaaac ggatatatct agtgcatatc gacgacttaa aagcccgaat
    13621 attaaaacta gaaaaagggc attaaaaatt atcaaagaac ataaacgaaa taaaatgaag
    13681 aaattagcat aattaaggaa ggaaaaagaa tgaagaaaat cagtgtagga cttattggga
    13741 tagcagctct aggattattg ggagcttgct ccagcactaa tgatgcaaaa gtatcgaatg
    13801 ataaagatgg aaaattagaa attgtaacaa cattctatcc aatgtatgat ttcaccaaaa
    13861 acatcgtcgg cgatgaagcg aacgtggatt taatggttcc agcgggatca gaacctcatg
    13921 actatgagcc ctcagctaag gatatggcaa aagctcatga tgctgatgtt tttgtatatc
    13981 ataacgaaaa catggaatca tgggtaccaa aagctaaaga aagttggaag aaagctgggc
    14041 ctaatgtagt agaaggaaca aaagacatga ttttattgcc aggtagtgaa gaagaagatc
    14101 atgatcacgg agaagaagat caccatcatg aactagatcc tcatacgtgg gtttcaccca
    14161 aaatggcaat taaggaagtt tcaaatatca aggatcagtt agttaaattg tatccgaaaa
    14221 aagcaaaagt atttgagaca aatgctgaaa agtatttgac taaactgaaa cggttggatg
    14281 ctgactatac cactagtctt aaagaagcga aacaaaaaag ttttgtgacg caacacgcag
    14341 cctttggata tttagctttg gattatggat tgaaacaagt tcctattgct ggactaagtc
    14401 ctgaagaaga gccatcttct ggtcgattag ctgaattaaa agagtatgtt aaaaaaaata
    14461 agatcaatta tatttacttt gaaaagaatg ctaatgataa aattgcaaaa actttggcca
    14521 acgaagctgg gataaaattg gaagtactga atcctcttga aagtcttaca aaagaacaaa
    14581 tggataatgg tgaagattat gtttcagtaa tggaagacaa tctaaaagcg ttagaaaaaa
    14641 caactatggt tgctggtgaa gaggtagttc cagaaaaaga agcgaaggat gaaaagacag
    14701 ttgctaatgg gtactttaaa gatgccgatg tgaaggatcg cgaattgagt gattatacag
    14761 gcgaatggca atctgtttat ccgttgttaa aagatggaat tttggatgaa gttttcgatt
    14821 acaaagcaaa aatcaataaa gacatgacag ctgccgagta caaagattat tatactactg
    14881 gctataagac agatattgat actatcaaca ttaaagacaa caccattgat tttgtagtca
    14941 acggtgaaca tcatcaatac acatacaaat atgttggata caaaattttg aattatgaaa
    15001 aaggaaaccg tggtgttcgt tttaattttg aaactgatga tgcaggtgct ggtcgtttta
    15061 aatatattca atttagtgat catggaattg caccaagtaa ggcagagcat ttccatattt
    15121 tctttggtgg agaaagtcaa gaaaaactct ataatgaatt gcataattgg cctactttct
    15181 atccagcttc attgtctaaa catgaaattg ctcaagaaat gatggctcat taaaattcaa
    15241 tttttggagg aaatacatgg taagaaaagt attattgtct atggattcca agaatattga
    15301 tgcttatcgg cgttatcgat ttgcaaagca aatgagatta aatgggagta cattagctga
    15361 tattgctgca actttacatg ttagtattga cacagttcat agagtattaa aacgggatcc
    15421 tgattgttgt tttatcaagg tcaatactgg aagaagtaga cgtttagcaa aacggcataa
    15481 tagcttggcc aattaattga taataccaga gtagatcatt tttcccttat ttggatttga
    15541 tctgcttttt attatgtctt gaattgtcaa atcaagcgca acactttatc aaagaagact
    15601 tcataaggtg ttctccaatc taaacacttt ctaggtctgt tattcagttt ttcaatcatc
    15661 tgatttatcg ttgagttatc taccgtagct atctctttcg attttggcaa gtattctcga
    15721 atgagtctat tcgtatttta atttgttcat tgttgccaag ggcatgagga tctggaaaat
    15781 aaaagggcat attattcaat gcttctgtaa ctgcgaaatg attggagaat tcttttcctc
    15841 tatctggagt gatcgttttg accgcctcat ttggaatact gcttaaaagt tcaatcattt
    15901 tctcgctcac ataagcggct ttctttctgg ggaccttctt tcctgctaaa aggaagcatg
    15961 attttctgtc tgtcaaagtc actaaacaag taccaccagt tttgccagct acaatatcgg
    16021 ctttccaatg accaaaatca cttctatcat ctgcggattt tggacggcca tggatcgaat
    16081 gagtaaggca aatctttcca cgagactcga catgattttt tgtatgtctt gtttttcctc
    16141 gatggtgcaa tgaacggatc aggcctttat tgccgtgtga gaggggttct gtatcgaaaa
    16201 gatcattgca aattgcacgt tagatcgtga taaaactaat tgaaaagtcc atatgttcat
    16261 gcttcaaacg attcgagatt tcctctggcg accactgaaa atcgacaaat aaatgacgaa
    16321 caattgacca aactcacgga tcatttagaa tgtgataacg tccgcatttt tttcttcgat
    16381 aagcataatt tttttccgct ttggaaggtg aatagttttt ttcttgtttc ttctcagttc
    16441 atgtaaaata gtaaacgggc tatgctgaat gtcgttgcca attatactaa tagaaaatcc
    16501 ttcttcgtga agcagaaaga ttctttctcg ttcgagtata gtaagatgac gatactggct
    16561 cataattatt ttccttctag gagtgttctc gacaaactta tactagttgg atatcgatta
    16621 tgactcattt ttatgtgttg cacttaaagt gtaaattcaa ggtatagaaa gaggttccct
    16681 atgaaaaaaa tacctattac aatcttaact ggctatcttg gttcaggaaa aacgatactt
    16741 ctaaatcata ttctgtacgg tgatcacgga aaggaaattg ctgtcattgt aaatgacata
    16801 ggggaagtaa atgttgatgg ggtattagtc gacaagaaag gttttaaaag aactggagaa
    16861 aaacttgttt cgatgccgaa tggatgcatt tgttgtacat tacgcgaaga tttgattgtt
    16921 gaactagatc aattagtgca attttctgac ttgaatcaaa ttgttattga agcttcagga
    16981 atcagcaaac caattccaat tattcaagca attatattag ctaaaaaccc tttagacatt
    17041 gatttgaccc aacgattaga gattggtgcc attattatag tagtggatgt aaaacgtttt
    17101 tgggataatt tcgaaacggg tgaaaagatt caggaaagaa aagctgaagg taatgtagtt
    17161 gaagaagaac aggatattcg tggcttgtta attgatcaat tcgagtgttg taacatatta
    17221 cttttaaata atagtgactt agtgtcaacc aagacgattt acaaaattaa cggtctaatt
    17281 gctaagttac agtccaatgc caaaataatt aggacaatta aaggtgaagt agacttatcg
    17341 aaaattctaa atacaaatct ttttaatata gatgagactt cctattcagt gggctggttc
    17401 aaagagttag agcttggcta tcaaaatcat attcctgaaa cagaagaata tggtatctca
    17461 agttttatct atcgaaatcg gttactcctt gacgcacaaa aatttgccca tcttacggat
    17521 aaaaattttc ctggcaatat tacaagagca aaagggtttc tttggtttgc ccaacatagt
    17581 gatttttcga cgatctttga aacatcgggt agctataaaa gtattgaatc attaaattat
    17641 tgggtagcta gtttacctaa aattgagtgt caaatgatta tacaaaaaaa tcccgaattg
    17701 aaggaagcct ggcatccgtt gtttggtgaa cgcattaata aactagtttt tattggtatg
    17761 aagatggatc aacaatgtat taaggaacaa cttgagggtt gtttaactga ttctcttaat
    17821 gcagataatt tccgaaaaat ttcaccattt ccatggaaaa agtctaatat gtttagccag
    17881 atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga taggcgatgt cataattatt
    17941 ggcattttga ttattacctc ttttattccc ttatttgtat tctctataga aaaggagaat
    18001 gaaacaactg aagatgctat caaatatgcc atcgttcgaa ttgatggcaa ggaaatggat
    18061 cgcttcgatt tggataagat caatcataaa ctcgtgaatt atcatcctgc gaagggacaa
    18121 tataatattg ttgagattaa agatggaaga attcgagtta aagaagataa tagtcctgat
    18181 caaatcgcgg ttagaactgg atggatctca aaacccggtc aaacgagtat ttgtttaccc
    18241 cataagttag ttattagtat tgagaaaaaa gaaagcacag attactacat ttactaatct
    18301 ggaaatagat gaggaggttt ttaataagaa aaagatatta ttattaataa tagcaggggc
    18361 tttattaagt gtgttaagcg cttgtggaca agaaaaagaa gcggtgacaa caggcacaag
    18421 tatctttaac gggacctgca atggtttagt taacagtcgg ttagattact tattttggct
    18481 cttcgtcaat gagtgttggt gaagaaattc aatagaataa tcgagatact cacacggcta
    18541 gacagctatc ttgtctagcc gtgttgttgt tctggcttta gtcagttggg tagtattctt
    18601 agttttctta aaatattttc ctcgatataa aaagattcgt tctcggtagt tttgaaagtt
    18661 tctatagcca taggcaattc gttttagtac tttgatatgg ttattcaaac attctatagg
    18721 gccgttggaa tagggaacgc gcaacgctcg tttgatttct gtttggtatt tcttgaaggt
    18781 cgtgaacact ggttggtagt gctctggcaa ctccttgtag tcttctttta ggagcgcgac
    18841 gaattcagga acatcttgtc ttctgataac ggaaagaaaa tcctggtaga cttcgtagcc
    18901 tcgctttaag gaatcatcat aagataatag ccgatcgact atctctgctt ctgtcagatg
    18961 atctcgaaat gacggacgcc agtagcgatg ctgacctttc aattcccacg cattcttttg
    19021 aagtagtttc cagtatttct ttaagtgctt cgaccgacga tccccttttt taaaggtctt
    19081 cattacttga attcggtggt tggtaaaagc ccgaccaatg tgttggctaa tatgaaagcg
    19141 atcggcgacg attcgggcat tcggaaaaca ctctttcacc aatgtgcgat agggtgtata
    19201 gaaatctgaa accactagac gcacttgttc acggactttt atagggtagc gcaagaaata
    19261 gtcgcgtaaa ggattgagtt gccgattttc cacaatatca atcagttgat gggtcttgcc
    19321 gtccatcatg acaaagctca tggctcccga gacatttttc actgatttga attcatcgaa
    19381 acacaccact tctggtaaca gctggctcaa agaaatggtt ttaggccgaa ggctgcgaag
    19441 tattcgaatg acagtggtcg gagaaatgtg cttcatacgt gcgatcaaag acatagaaag
    19501 aggttcgctt aatagctcaa gaatggcttg tttgactctg cgagcaatgg agcagcgacg
    19561 atcactaact gaatcttctg ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg
    19621 tttgttaatc cgcagaatgg tcttgtagtt actgacatca ttcaataaga tggatacttt
    19681 tttccagccc tatttaatga tcgtttgttc attttgatag tggcgatgaa agcattcctc
    19741 cggttgatag gtcaatgtgc catcaaaagc aaaacatgtt tcgtcattga tgcgctcttt
    19801 gcgaaaggca tcctcatgaa aggtaagatt taggtctaga atatctagta actctttggt
    19861 aaactctgaa taggacatct tgaaacacta ctttgaatgg tttttgtcga ctttattcta
    19921 aaggatttta aggtgtctgt ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa
    19981 ggcgtcacca acactagata ttatagatcc cttattttct tcgcttcaaa ttgtcaagga
    20041 gtcatcataa attttgtatg gattcttttg tttgggtagt agtcttctaa ataatcttgg
    20101 attaacacct tcatctctat tttactttta tatttgttgg agtcgattaa ttctcatttg
    20161 attgatcgat gaaacgcttc aattactgca ttgtcaacag gagtcccagt ttggctcact
    20221 gaatgtgtga tgtggtggtc gttcaacagc tcttgataaa taaaagaccg atgctggcta
    20281 cctctatcgg aatgaattac cgtaggcaat tctccttaat aaatttgtaa tggacggacg
    20341 acaatctctt tttttatgtg tgaatctacc atatagtaac gtagaatacg agttgctaag
    20401 tcaatatagg tggacaaata gaggcgaccg tcattacatg gaatgtaagt aatatccatc
    20461 acccaaacag catcaatttg gttctgttca aattatcgat tcaacatttt tttaccattt
    20521 ctttgattgt tttttctcta ccatgagaag atttgcgtcg atgaaatcct cttgtgcata
    20581 aattcatttc acgcataaga acagctacaa cacgtttatt gatttcgatt ccttcatcgt
    20641 aaagtttttg agtgatccct ggagatccgt atcgatcttt gtgttcgtag aacactcgtc
    20701 ttcctctttt agtcgtttat tttcctgttc aagaatgtag aaatctgctt tatcaggtaa
    20761 tctttttcct tgtccagcaa aagcattttt cccgtatcga cgatattctg aaacccatcg
    20821 ggaaattgtt ggacgagaga tattgtattc ttcagcaatt ttcttggtag ttactttttt
    20881 ctcaaggata tcctgtgaaa tctttagttt aaatttacga tcatatgttt ttctcaatag
    20941 agttcattcc ttccggtaac gtaactgata gtcaagaaaa gtgtatcata gcccaactta
    21001 gtatcagggt ttgtcaacta aactgtggaa gttgatagat aagagttttt aacaaccaaa
    21061 attctcttga ctatttttga aatcagacaa attttgattg gacacatagt tgaataaatc
    21121 taccgtcgtt acggaaggta aatcgcatgc ttttcaattc tagaggagaa gtcctcgttg
    21181 aagtaccata gcccagttct ggattcgacc tcctgcccac ttcatacgta aataaagtaa
    21241 ttttaggagt gagactgtga caaaagttat ttttaagcta aaaacgaatt aaaaaacgtt
    21301 gataattaaa atcattcgtt ttagattcta tgaatcatta tagaacgctg taaaatcgat
    21361 actttataat tataaagaag atagtttcaa atttattgtt cgaaatttga aactatcttt
    21421 ttacttatgt cacagactcc cttagttgag ttattcttta atttcgaatt tcgagatagc
    21481 tagatatatt gcaagcatac attcttgata acaatcttga tcatattttc cagttctcca
    21541 ggattgctta tgaatcaaag agctaaatct tttgagaatt gcctcaacag cttcttcatt
    21601 tccagccttt gctaaaatga aatcttcttt taaagaattc ataaatctac cttcttccca
    21661 tggacttata atctatttct ttgggaagga ggagaacgag caaagaagca tacaaaatta
    21721 gttattatgc agatattaaa ccagcatttt ttgaattttt ttgtaaagtc gatgttttaa
    21781 atttgtcgca ccctgtcttg taatccccag aacctttcca atttctttat cagttttatc
    21841 aaagataaat ttttcgataa gaaaaatttg ttcaggttct ttcaacaaag gaaggatttt
    21901 tgctaatttt atatcttcaa caaggagagt cacatttaaa acattaatcg gttgtttaaa
    21961 ttttatagta tctttagtaa acaaatatgg tgggagttga tcaagttgtt catgtttttg
    22021 aatttcttct tgttttttat aataagtatt agcggtgttc aaaatgacct tttcaaagaa
    22081 tcgaatcaac tgggcattta cgcgttcgtc gttatctgac atattgcaca cctcctccat
    22141 taagatttaa agggaaaaga ggcaaaaaac acgaaaagaa gctaatgcaa ttatgctgcg
    22201 ctgcgaataa cttctataag ctcacatgca ttgataggac tgctgatgtt tattttcaaa
    22261 aatgtttttt tagttgataa tggatagtta tctttatact tagaaaagaa aattcatata
    22321 tcttcttaaa tatcaaacat gtccttatgg ctaagaaaat gtcttgagct ttttacgtat
    22381 aataaatctt ttctaaaacc tccttttgga taatattctt attcgtgtat agattgtatt
    22441 ttttatttct tcccccttca taattaacaa ctagaaaaga gttcgaatag acacgtattt
    22501 gaacgaaaat ttttattgga ttcccaaatt ggaatattta gaaacgaaaa attcctatta
    22561 tctgtatagt gcttagataa taacggatca taataattat tgtctttgaa ctagatatat
    22621 gtaaatagta ttcatcatga tgaaaaatga tgagttctcg gtgataaaat gtacttttta
    22681 cggtggaata tacatgaaag cgataagtgg atattttata atgaagtaag ttagcttatt
    22741 ttgacagagg aatagtattt tagataacgt ataaaatact atttaatgat gtagaaagga
    22801 aagagagaaa caaaagtgtt ttcagataaa gaagtcttat taaagtctct tcatgtttgt
    22861 ttaaagttac ctatcgtggt ttttaattcc gaattcagta ttataaaaga gtatcgctca
    22921 gatagaacta cttatttttt ttatgattat aaaaagttac taactgataa tattaaaaaa
    22981 actaaagatt tccatttctt tactggtgat tttaatgaga tgtttcttct atacatgttt
    23041 aaaaccagat attacttatt tggtccattt agagcaaata atattgataa agtttttttt
    23101 aaactaaaaa tgaacaattt gaatgttgca atggctgata gagaacgact ctataattca
    23161 ttgcaaaatt tgactttgta ttctttagga gacattcgag atattctgat tttagttcat
    23221 tattttttta ctggaaaaat agaagatcta ttccatgaac cgttaattga atatacagaa
    23281 aatctaagta aaactatcga gcaaatcaaa atagataatc tgttatcaca aaattacgat
    23341 ccagaaatat atctgtttct ttatgaaaat aaaatactgg aatatgttaa aaatggagat
    23401 attcgaaatt tagaaaacat ggtttttaat ctgagtaatg gaataattcc aagtgttagt
    23461 ggagatacga ttcggtctga aaaaaattat tctatcattg tttttgaaaa gttagcacaa
    23521 acgtctataa cattaggaat ggatataata gaagcgtatc aaagtagaga tgctttgata
    23581 caagaaaacg aattagctgt atcgttacct gaggtcttaa aagtgaggga ttctgggatt
    23641 gtttattata ctaaggaaat cggaaaaact aagatagagc atctttctcc cttaatttct
    23701 tctgtcgttc aattcatcgg cttaaatatc tataaaagaa ttactgtaaa ggaaatagct
    23761 aattattttt ctgtaagtga aactaaatta agagaaagct ttaagaatga aatgcatatt
    23821 acgatttata attatatctc taagcgaaaa atttctacgg ctaaaataat gttgaaatca
    23881 aaccatacta tcagtgaagt ttctttaggt ttaggatttt cagattcttc acacttttcg
    23941 agagtattca aaaagtacgc aggtgtatct cctaagcaat atcaattagg tttagtggac
    24001 aatttcaaca acattagctg agaggtttta ttttattatg agactatatc atttacgtag
    24061 tttggaggag ttgaggaaat tggattttaa caaaaaattt ttttgggaaa tttctgattt
    24121 gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata
    24181 tgacaaagta gtaattaata aagtaaaaag tttatgtgaa gaagctagag aatctatcag
    24241 tcatattaat ggtagcaatt ttaaacaaac attatttaat ctgattaagc tagatgctaa
    24301 aatatcaagt tatttattct ttttgataca tgatgaattt atggattatc aaaaattgga
    24361 tcagttaatt gaaagagaaa gctgggaaga ttattacgtg ggtctaactt tttcagaaaa
    24421 gttaaacaac tacaagttaa tagattataa gtatttgagt gaaagcaatt aattattcat
    24481 gtagagtact agacacttta aatatggaca attcaattat aaagaaaaag aatcaataat
    24541 ttaatgaggc tgtgacaaat gtcatttttt gtttcgctaa aaacgagtga aaaaatgttg
    24601 ataactccaa tcattcgttt aagattctag gaatcattca gaaatgctgt gaaaccgata
    24661 ctttataatt ataaagaagg tgatttcaaa ttccgaagaa ggaatttgaa gtcacctttt
    24721 tacttatgtc acagtctcat tcagtaatgc gggataaggc tccttattcc gttatttgaa
    24781 tactctgaag tttaatctaa gaaagggtaa tattttgaaa acatgcttca tcgcatagaa
    24841 atgaacttaa tttcagaaag aatacataca tatcttttta tatgttttca gctgctgaat
    24901 ggttaaaaag cacaaatatc cgaaaaaaac atgaagaaat gtactttttt tgttgtaatg
    24961 ttcatgaaat tctctaagta aattagttaa cataaggtta gtaagattcc tattctgtga
    25021 ataatacgta tcagatttat cttttattct tactaggaaa gtaattatta agggagttga
    25081 tttgaataat ggttagcaag aataataaga gagtatttct agaaaaaaca aagaaacgag
    25141 tattgaagta cagtatcaaa aaattaagtg ttggggttgc atctgttttg attggtgttg
    25201 gtcttgtctt gggaacaact gaactagtta aagcacaaga tgaaatttct ccaagcagcc
    25261 cacttgaaac agcgataagc tccgtacaag taggtgacaa agtagcttcg gggaatactt
    25321 ttcaggagaa tcctggttat acgaaaaatt ataacttttc ggatttacaa tttagtcctc
    25381 aagaactaac tggagacaca ctaaaaggca atacaattgg atttgaagtt tacggaaaac
    25441 ataatattgc agcatcaact aaaaactggg aaatccgtct tcaattagat gaacgattag
    25501 ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg aattggatct tctagacgga
    25561 cctttgtaag aattaatgat tcgctgggta gacctacaaa tatttggaag gtcaattata
    25621 ttcgtgcaag tgatggatta tttgctgggg cagaaacaac cgatacgcaa accgctccta
    25681 atggggtaat tacgtttgaa aagagcctag atgaaatttt taaagagata ggcatagaca
    25741 atctcaagac cgatcgttta atgtatcgaa tctacttggt aagtcatcaa gacgatgata
    25801 aaattgtacc gggtattgat agtacgggct actttttaac agattcagat gatttctata
    25861 atagcttaga tgtatctgaa aataatcctg accagtttaa acatggatca gtaagtgcaa
    25921 aatatgagaa acctaatact caaacaaaag atggttcagg atccacaggt gctaatggag
    25981 ctataatctt agatcataag ttaacgaaaa attataattt ctcatattca tcttccgcaa
    26041 agggcacgcc ttggtatgct aattacaaaa ttgatgaacg attagttcca tatgtagcgg
    26101 gtatacagat gcacatggtt caggctgaca aagtaacata tgatgtttcg tttgaatccg
    26161 gaacaaaagt agctgattta gcaatcgagc gacgggaagg tcatgaaaat tatggcatag
    26221 gttcaataac taataatgat ttaactaaac ttatcgattt tgctaatgca agtccacgtc
    26281 cagtcgttat tcgatatgtt ttacaattga ctaagccatt agatgaaatc ttagaagata
    26341 tgaaagcaac agcacaagtt gaagaaaata aaccatttgg tgaagatttc atctttgatt
    26401 cttggttgtc ggatacgaat aaaaaattaa tccagaacac ttatggaaca ggttattatt
    26461 atttgcaaga tattgatggt gatggaaacc ctgacgataa agaagagagc ggagacacga
    26521 atccatatat cgggaaacct gaattagaag aagtatatga tgttgacaca acagttaagg
    26581 ggaaagtatt catccacgag ttagcgggaa caggtcacaa agcccaactt gttgataaag
    26641 aaggtactgt attagcagaa aaaactatcg ctccaaatga aaaagatggg gctccaattt
    26701 cagatactgt agaatttgaa tttacgggtg tagattcaag taaattaatc gcgaaagatg
    26761 aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt tcaaccgtta
    26821 ttaaggaatc accaaaagcg gttgataaac aaaccgtggt agttggattt aaaccagatg
    26881 ctaaagaatc aattcggaat aataaaaact tacctgaaga tgcagagtat tcatggaaaa
    26941 cagagcctga tacttctaac gttactgata gtacgaaagg tattgtaact gttaagatcg
    27001 gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa
    27061 atgatgcaac atacgtacct ataacaacaa ctccagaaac gacagttcaa agtggaaaac
    27121 ctacatttga taaaccagat gttcctttag ctaaagatgc cttttcaatt tcagatgtct
    27181 ataataagga cttcggtaat gcaagtgttg atgcaaacac tggtattgtt acattcactc
    27241 cagctaaagg tgtaggagaa tcggagccga ttactggaac aattcctatt aaaattgttt
    27301 accaagatgg ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa gatatttatg
    27361 aaaatccagg agaaaacatt cctgcaggct accacaaagt aaccttcacc gcagg
//
LOCUS       DAFELO010000037        27335 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFELO010000037
VERSION     DAFELO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.037"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(88..456)
                     /gene="rplL"
                     /locus_tag="LP452_001657"
     CDS             complement(88..456)
                     /gene="rplL"
                     /locus_tag="LP452_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL2014385.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(509..1012)
                     /locus_tag="LP452_001658"
     CDS             complement(509..1012)
                     /locus_tag="LP452_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL2014386.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1216..1343)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1422..2111)
                     /gene="rplA"
                     /locus_tag="LP452_001659"
     CDS             complement(1422..2111)
                     /gene="rplA"
                     /locus_tag="LP452_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL2014387.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2215..2637)
                     /gene="rplK"
                     /locus_tag="LP452_001660"
     CDS             complement(2215..2637)
                     /gene="rplK"
                     /locus_tag="LP452_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL2014388.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2980..3648
                     /gene="sdaAB"
                     /locus_tag="LP452_001661"
     CDS             2980..3648
                     /gene="sdaAB"
                     /locus_tag="LP452_001661"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL2014389.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3661..4533
                     /gene="sdaAA"
                     /locus_tag="LP452_001662"
     CDS             3661..4533
                     /gene="sdaAA"
                     /locus_tag="LP452_001662"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL2014390.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4624..5643)
                     /locus_tag="LP452_001663"
     CDS             complement(4624..5643)
                     /locus_tag="LP452_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL2014391.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5816..6721)
                     /locus_tag="LP452_001664"
     CDS             complement(5816..6721)
                     /locus_tag="LP452_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2014392.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(6780..7325)
                     /gene="nusG"
                     /locus_tag="LP452_001665"
     CDS             complement(6780..7325)
                     /gene="nusG"
                     /locus_tag="LP452_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL2014393.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7426..7596)
                     /gene="secE"
                     /locus_tag="LP452_001666"
     CDS             complement(7426..7596)
                     /gene="secE"
                     /locus_tag="LP452_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL2014394.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7620..7772)
                     /gene="rpmG"
                     /locus_tag="LP452_001667"
     CDS             complement(7620..7772)
                     /gene="rpmG"
                     /locus_tag="LP452_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2014395.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7934..8578
                     /locus_tag="LP452_001668"
     CDS             7934..8578
                     /locus_tag="LP452_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2014396.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8634..9137)
                     /locus_tag="LP452_001669"
     CDS             complement(8634..9137)
                     /locus_tag="LP452_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL2014397.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9150..9196)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9734..10699
                     /locus_tag="LP452_001670"
     CDS             9734..10699
                     /locus_tag="LP452_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL2014398.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10798..11970)
                     /locus_tag="LP452_001671"
     CDS             complement(10798..11970)
                     /locus_tag="LP452_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014399.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11957..13102)
                     /locus_tag="LP452_001672"
     CDS             complement(11957..13102)
                     /locus_tag="LP452_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014400.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13116..14048)
                     /locus_tag="LP452_001673"
     CDS             complement(13116..14048)
                     /locus_tag="LP452_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014401.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14165..15244
                     /locus_tag="LP452_001674"
     CDS             14165..15244
                     /locus_tag="LP452_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2014402.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15241..15870
                     /locus_tag="LP452_001675"
     CDS             15241..15870
                     /locus_tag="LP452_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2014403.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15980..16207)
                     /locus_tag="LP452_001676"
     CDS             complement(15980..16207)
                     /locus_tag="LP452_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2014404.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16310..18898)
                     /locus_tag="LP452_001677"
     CDS             complement(16310..18898)
                     /locus_tag="LP452_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL2014405.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18918..19577)
                     /locus_tag="LP452_001678"
     CDS             complement(18918..19577)
                     /locus_tag="LP452_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2014406.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19626..20129)
                     /gene="trmL"
                     /locus_tag="LP452_001679"
     CDS             complement(19626..20129)
                     /gene="trmL"
                     /locus_tag="LP452_001679"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL2014407.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20350..21216)
                     /locus_tag="LP452_001680"
     CDS             complement(20350..21216)
                     /locus_tag="LP452_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2014408.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21325..21966
                     /locus_tag="LP452_001681"
     CDS             21325..21966
                     /locus_tag="LP452_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL2014409.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(22028..23389)
                     /gene="mgtE"
                     /locus_tag="LP452_001682"
     CDS             complement(22028..23389)
                     /gene="mgtE"
                     /locus_tag="LP452_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL2014410.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23407..24306)
                     /locus_tag="LP452_001683"
     CDS             complement(23407..24306)
                     /locus_tag="LP452_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2014411.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24308..25105)
                     /locus_tag="LP452_001684"
     CDS             complement(24308..25105)
                     /locus_tag="LP452_001684"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL2014412.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25083..25787)
                     /locus_tag="LP452_001685"
     CDS             complement(25083..25787)
                     /locus_tag="LP452_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL2014413.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25918..26493
                     /locus_tag="LP452_001686"
     CDS             25918..26493
                     /locus_tag="LP452_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL2014414.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26611..27288
                     /locus_tag="LP452_001687"
     CDS             26611..27288
                     /locus_tag="LP452_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL2014415.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7976 a         5761 c         4921 g         8677 t
ORIGIN      
        1 tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaacatag
       61 gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc caacttcttc
      121 aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa ctggtgcagg
      181 agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt cacgaactgc
      241 tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga attctgtttg
      301 ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag cagcagatac
      361 gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag ttgcttcttt
      421 taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg tttaattgtt
      481 cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct ttgtctgcca
      541 ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat agcaacatag
      601 atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc aatgaagaga
      661 ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta gcaaattcgt
      721 ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct gtaggtccag
      781 tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa agaatagaat
      841 ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa cgtgttactt
      901 catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct gtaaattttt
      961 cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc atttttgagt
     1021 ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt cacttttata
     1081 gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag tgattctact
     1141 cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc atcatctctt
     1201 cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca tagagggact
     1261 attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc acctactgtc
     1321 ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag ttgagtcaag
     1381 aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga agcttggtca
     1441 acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta ttgaccttta
     1501 gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt ttcaactaat
     1561 ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc tttgtccaca
     1621 cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc cattgtaact
     1681 gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc tagacgacct
     1741 acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca tccgttttgg
     1801 attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc agcttctttt
     1861 gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc gtttggtaat
     1921 acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa acggtatgca
     1981 acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg
     2041 gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat ttttttgctc
     2101 tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct cccacgtgtt
     2161 ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta tagattattc
     2221 tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt
     2281 agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat cgctagaaac
     2341 tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag ctgctttttt
     2401 caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac
     2461 agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt
     2521 tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg gtggagctgg
     2581 agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct ttgccacgag
     2641 acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc ctcccacgtt
     2701 ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac tggtacagta
     2761 tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt tgcttgaata
     2821 aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc ttttatgtta
     2881 cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt tttagaccct
     2941 tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt aaggtataga
     3001 agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca cactgccgga
     3061 gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga ttctgtagat
     3121 atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga tattgcatta
     3181 gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc tttgaaattg
     3241 gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca
     3301 aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac tggcatttcg
     3361 atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc tttaagcatg
     3421 ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc taaagtgact
     3481 agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg ggaatcaaaa
     3541 ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga aacggtaaaa
     3601 agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg agctaaaaaa
     3661 atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag tgtagcagaa
     3721 ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat catcgagatc
     3781 atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga aggtgttacc
     3841 tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt aaatgaggca
     3901 gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa
     3961 gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc gggtgttgtt
     4021 cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca gcaattggaa
     4081 ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt
     4141 gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc agcagctttg
     4201 gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat tacattgaaa
     4261 aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa
     4321 cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt agcgggtatc
     4381 gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg
     4441 ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac tgctaaacga
     4501 cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc gagtattaag
     4561 agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc tcttcgtttt
     4621 ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc ctgcaagttg
     4681 catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc
     4741 acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag caccacttgc
     4801 cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca ctggtacacg
     4861 tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac ctccatctaa
     4921 ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga tagcgattac
     4981 tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga tttctttgat
     5041 tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt ctgcgattcc
     5101 ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt taggcattgg
     5161 taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc
     5221 tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa tgaactcatt
     5281 gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc
     5341 ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg atccagcagc
     5401 agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt gggccgctga
     5461 tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat gcaaatcggc
     5521 tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag aagtcgtatt
     5581 ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag ctcctgcttc
     5641 catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga ttggatgttc
     5701 ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga ttacatataa
     5761 atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt tatcgctatt
     5821 ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga agcatactat
     5881 gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt tgcttcaaca
     5941 acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag tctgtttcat
     6001 ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg atcatttgct
     6061 ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca tctgtcagag
     6121 actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa
     6181 cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca ctaactcctc
     6241 ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc agatacaaaa
     6301 gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct tcaaacaata
     6361 cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt atttgtccat
     6421 ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc aaacgccgca
     6481 acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag
     6541 tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa acttcagtag
     6601 tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg actaaagctg
     6661 ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc ttcctttcca
     6721 tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa gtctctttat
     6781 taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg tccaaacata
     6841 tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg tccttctaat
     6901 ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc tagatcattt
     6961 tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg aagtaatggt
     7021 gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg
     7081 acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt
     7141 ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc ttcaggaaca
     7201 acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc gatatttgct
     7261 tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt ttcaaaagat
     7321 tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt
     7381 tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca
     7441 gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga aaataataga
     7501 agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc aagttacttg
     7561 tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct tgctactgtt
     7621 tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt attgatttcc
     7681 aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga cccacaaacg
     7741 gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc tgagaatcag
     7801 acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga aataaactat
     7861 ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa atgagacaat
     7921 tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc tgacaaccgt
     7981 tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg gataccgttg
     8041 tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca aaatgcatat
     8101 ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa
     8161 cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta cgatcaaaga
     8221 gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt tgacacatag
     8281 tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct atgtactgac
     8341 aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca tttcaaaaca
     8401 cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca ttcgacgtac
     8461 attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg tcaaccactt
     8521 agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag aagcataatc
     8581 aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta taattagaca
     8641 cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga aacagccatg
     8701 acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt
     8761 ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt gttcaatact
     8821 aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc taaagaaagc
     8881 aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa ggcattatag
     8941 acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac taaatgattc
     9001 aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac taaaatagtt
     9061 aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa ccgttgtctg
     9121 cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg cgggatggtt
     9181 gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat ttatttcaat
     9241 ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc
     9301 gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa
     9361 gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag gatgtgggaa
     9421 tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta agtggacatg
     9481 ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta ggcttttttg
     9541 tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat aaccaagaat
     9601 taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag
     9661 cttgctaatt attggttaac cagcaaacag gagtattaaa atatcttgtt ttattgaata
     9721 gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg caggcagcgg
     9781 aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc atattgacta
     9841 tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa ctttggcaaa
     9901 agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt tccccctttt
     9961 gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct atcagatgga
    10021 agtagaattc ccagttgctt atattccagc aggttctgga aatgattttg ctcgaggggc
    10081 aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc aatcaccaga
    10141 aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa
    10201 taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt cttccacaaa
    10261 aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta ttctccgtgc
    10321 tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac aattaagttt
    10381 ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag gaatcgcgat
    10441 tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa
    10501 tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat tgaaatcgaa
    10561 atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac cgcaacatgg
    10621 tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa ccacaactaa
    10681 acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt
    10741 attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac tatgcagtta
    10801 attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa taaattggat
    10861 cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa aagattctgc
    10921 aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa caaaccaata
    10981 tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg ctggtacaaa
    11041 caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac cgctggtttc
    11101 tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca ataaaaacaa
    11161 gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga tcactaacag
    11221 aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca aactaaaggt
    11281 tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt tactgatctc
    11341 tttgcgatta aacgctagat tgatgacgct gaaaccgcta aaaatggata gaaacaaccc
    11401 ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct gactatatcc
    11461 cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc
    11521 atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg
    11581 ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga tcgtcgccat
    11641 ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg
    11701 attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg taacgactgt
    11761 ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt cttcactaat
    11821 caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa
    11881 gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat ttgctttaag
    11941 caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc caagcatttc
    12001 ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa aaactagcca
    12061 aatcagtgta gaaataaagt ttatacaaac tttcgctgat caagttgacg agatttaatt
    12121 gaccaacaat cggaatatga agatcaatcc aatacttcac ttgttccgac cccatcatac
    12181 cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat gctatccttt tgcaccagat
    12241 tcatttttgg tatcaaatta ccaaccaatg tacctaacaa aatcgcacac aacgacccca
    12301 gagcggtaac aatcatcaac cattgccaac gttcaccaaa gtctacttga tagataaaac
    12361 gaaagacagc taacataata taaatctctg tcaaaacaat cgtaaagctt gctgccaaat
    12421 tagcgattga aactaacgat tgctttctag gaatcaagga caagcgaata ccatttgacg
    12481 attgatttgc ttgctgatcc tgtacattgc ggacccccca catcgtcccg tagagaatcg
    12541 ccattccaat cagagtgaag aaataaaaac ttttgaaact catattacga cctgcatgaa
    12601 cttcttgaaa agatacttct tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct
    12661 ccacttctgc cactttactc gaaattatac caatacgctg taaaaattga tccatgacgt
    12721 ttttcatcaa cgtctgactc attcctgctt gacccacatg gagcgaaaca gatgaaccat
    12781 caatgagata aaaaccagcc aaatcatctg catttagctt ttcattcgct tctacttccg
    12841 atagttcctg tacttcaaat aatggtttat cattgacttc gacttgttct aagactgtgg
    12901 taaaattttc cgaaagcgct gtttgatccg ctttgattac aattccagta ttaatcgttt
    12961 caagattgcc accttgatca atctcgccaa aggccgccat aaacatcaat cctaaaagaa
    13021 ttggaaaggc caatgtccaa aacaacatgg atttatttcg cagcagtatt tttgtgcgat
    13081 atttataaaa atgccaaaac attatttccc ctcctctagt ctctcaaatc cttgccagta
    13141 atctctaaaa agacatcatt caaggacgct tctttggttg ctatttttcc gtaactgatc
    13201 ttttcttctt gtaagattgt aagaatcggc aacaatactg tatttagttc agtagttgtt
    13261 acttggagct gacctttttt ataagcgatt ccatcagaag ctattgttcg ctgaatcgct
    13321 gacaacacat tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
    13381 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
    13441 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
    13501 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
    13561 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
    13621 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
    13681 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
    13741 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
    13801 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
    13861 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
    13921 gtcgatttcc cactgccgtt tggacctaac agacctaaga cttctccttc ttgtacttgc
    13981 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
    14041 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
    14101 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
    14161 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
    14221 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
    14281 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
    14341 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
    14401 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
    14461 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
    14521 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
    14581 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
    14641 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
    14701 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
    14761 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
    14821 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
    14881 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
    14941 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
    15001 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
    15061 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
    15121 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
    15181 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
    15241 atgataacag tagtcattat tgatgatgat ccatttgtga cgaaatcgtt acaaaccatt
    15301 ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta
    15361 gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa
    15421 acgggcatcg atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt
    15481 ttgacgacat tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg
    15541 tatttaatca aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga
    15601 caggccgtat ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg
    15661 acgattgata gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa
    15721 gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact
    15781 gttcgcaatt acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg
    15841 gctatctatt tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga
    15901 tgctccttcg catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat
    15961 ttcctcacga ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg
    16021 gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac
    16081 gatcgtctcg ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta
    16141 actttttaaa gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt
    16201 tagccataaa gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa
    16261 aagaaaacgc taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt
    16321 ttgccggtat aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact
    16381 ggttatcgat cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt
    16441 ctttcttttc ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt
    16501 ggtttttcgt ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc
    16561 gtggctcttt tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat
    16621 aagctgctag tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt
    16681 tcaacgtagt catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt
    16741 cccctaacaa aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct
    16801 gaagcatccg ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt
    16861 gtgatttatg gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat
    16921 aagtgacctc atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt
    16981 ttgccaagat gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag
    17041 tattgactaa ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt
    17101 ccttttttcc aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga
    17161 tccctgcagg tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct
    17221 tatttttcgc tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc
    17281 ggaaatagga acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga
    17341 tctcatgggc aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt
    17401 ccattttggg aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag
    17461 agggaagctg atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga
    17521 ataatgtgtt agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc
    17581 cttctaattc tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac
    17641 gatggatcgt actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag
    17701 ttggtgctgc aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca
    17761 gtccgttcaa ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac
    17821 ctggtcctcc tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt
    17881 gcgaaggtcc atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt
    17941 ctttttcagg ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga
    18001 tcccccattc agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct
    18061 ttccttcttc aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg
    18121 ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg
    18181 tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc
    18241 tcggtgaatc tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga
    18301 ttccttctat gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt
    18361 tcttataggc ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc
    18421 ccatgatcac ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt
    18481 ttggcttcgt caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga
    18541 tagcatcttc tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga
    18601 atttttcgct ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat
    18661 atctttcgag tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac
    18721 gataagtctc attttcttga atatcgccga aacttccggt gactacaatc tctttttcac
    18781 ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat
    18841 tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg
    18901 gaagccgcct ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct
    18961 gtttggttcc atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa
    19021 atcgtcaaac tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct
    19081 gtcatccatt gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca
    19141 tctttatgtc gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca
    19201 atcgtttctc cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc
    19261 agattatccg gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc
    19321 cctagttctc ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg
    19381 ataccttctg ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga
    19441 acatatttta cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga
    19501 gaatcgccat tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg
    19561 gtaaaatata gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa
    19621 actgtctaat ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct
    19681 tgctggcgaa gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg
    19741 acatgctcat cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt
    19801 tctggcaaac cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa
    19861 ttttcgtcac tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct
    19921 aaaaatgtta aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa
    19981 tccaaccctg cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa
    20041 tgaagcggag tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt
    20101 aatggttcaa ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt
    20161 tctattttct gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca
    20221 ctgcgagtcg ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga
    20281 taaaaaccaa tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac
    20341 ctctactcat taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat
    20401 gtcaattgta atcttcttca aaggattctt atataagtct gctttgattc gctcttctac
    20461 ctgccattct aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa
    20521 ttcataggtt acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac
    20581 acgttcattg gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc
    20641 ctttaaataa ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg
    20701 ttggaattct tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc
    20761 agcaaacggc aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt
    20821 agctgcgtag actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa
    20881 ttttgaaagt tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc
    20941 ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat
    21001 tctacgcctt ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa
    21061 ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt
    21121 atcagacttt tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc
    21181 caaaaactgg cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat
    21241 cttaccgtta gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa
    21301 gtgaaagata aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct
    21361 attcctctag caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc
    21421 ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa
    21481 gatatccccg taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc
    21541 gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta
    21601 gtatttggtt gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc
    21661 gaaacagcag aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat
    21721 cggcaattcg aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat
    21781 ggcggtcctg ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc
    21841 tatacagatg gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca
    21901 catgtagcta aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag
    21961 ttataatctt ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt
    22021 tgttttttta cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac
    22081 tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta
    22141 gacgatccat tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata
    22201 gcatagccat ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta
    22261 gaaaaatcgt gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa
    22321 caagtcgaac aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag
    22381 ctagcgactg ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta
    22441 aaatactcgc ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac
    22501 ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt
    22561 cattaatttc ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat
    22621 caataacgtc gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg
    22681 taacaggaag agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt
    22741 cgcctacatg aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat
    22801 cattcacgat taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga
    22861 catagatgta atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag
    22921 agcgaacagt ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg
    22981 tttcatcctc gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac
    23041 ttaaatattt agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg
    23101 tatacatctc agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt
    23161 tcatatgttc gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca
    23221 gataggaata gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct
    23281 catagatatg catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat
    23341 cttgagtcaa ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac
    23401 ctcgccttat tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt
    23461 gaactccagc aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc
    23521 ttgtcttttt attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca
    23581 gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc
    23641 aactaacgcg atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc
    23701 ttcatcagag accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat
    23761 atgtccatgt gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac
    23821 aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa
    23881 aagcatcaag ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc
    23941 gtaaccttgt tttttataat aagcctttac acgattagcc attgtcccat ttggatggta
    24001 ttgggcagga atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc
    24061 atacacgata tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg
    24121 aatcacaatg gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc
    24181 attaactttg atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc
    24241 tttttctttt aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa
    24301 ctgcatatca atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc
    24361 gataagaagc aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg
    24421 cttttgcgac attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta
    24481 tttctagcca ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat
    24541 tattcagtga agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac
    24601 cgccgacact tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc
    24661 ctctaaaact ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg
    24721 cccgtttaat cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat
    24781 aactgatccg aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc
    24841 tgtctactag ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg
    24901 tatgcacgcc caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca
    24961 ataatgtgcc gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa
    25021 tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt
    25081 tattatgaac gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa
    25141 agcgttcgat agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct
    25201 tcttggattt cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc
    25261 gcacgctgaa gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc
    25321 gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa
    25381 attttcttct caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg
    25441 tagccacttg cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt
    25501 cttttacgga tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt
    25561 aacccggcaa tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt
    25621 gattttgtta caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca
    25681 tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat
    25741 acagtttgtt catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct
    25801 acttcattaa cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat
    25861 ttatgcattg accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg
    25921 acacaaatgc ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc
    25981 atcgctcact atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat
    26041 accgttgatg aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat
    26101 caagctgaat atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc
    26161 tttataacag aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca
    26221 gtttttcaaa aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag
    26281 cttacaacaa aacgagctga attccctatt gaagaaggac ttttagctat tgatgaaagc
    26341 tggggagaca atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga
    26401 aaaattgctt ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac
    26461 aagatccaac gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg
    26521 agttcatttt tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt
    26581 tgtatagaat ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct
    26641 ttaggggaat actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac
    26701 gggacaccat caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca
    26761 attggggatg tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac
    26821 ttattctcaa tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag
    26881 aaaaaaggcc gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca
    26941 tattctcaag aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt
    27001 aaagaagatc gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct
    27061 cgggaaatgg gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt
    27121 gattttggtg ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg
    27181 tgtaaaacaa cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat
    27241 tctcagttcc attcaagaac tagccattta cacttgcttt ctaattaaaa aaggctcttt
    27301 gtcaaatgat gttggtaaag agaaatctta gaata
//
LOCUS       DAFELO010000038        25937 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFELO010000038
VERSION     DAFELO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.038"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(1238..1903)
                     /locus_tag="LP452_001688"
     CDS             complement(1238..1903)
                     /locus_tag="LP452_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014416.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2083..4065
                     /locus_tag="LP452_001689"
     CDS             2083..4065
                     /locus_tag="LP452_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL2014417.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4069..4707
                     /locus_tag="LP452_001690"
     CDS             4069..4707
                     /locus_tag="LP452_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014418.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4735..5163
                     /locus_tag="LP452_001691"
     CDS             4735..5163
                     /locus_tag="LP452_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014419.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     EKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5620..6531
                     /locus_tag="LP452_001692"
     CDS             5620..6531
                     /locus_tag="LP452_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL2014420.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVENQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6748..7185
                     /locus_tag="LP452_001693"
     CDS             6748..7185
                     /locus_tag="LP452_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014421.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7280..8164
                     /locus_tag="LP452_001694"
     CDS             7280..8164
                     /locus_tag="LP452_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL2014422.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8388..11555
                     /locus_tag="LP452_001695"
     CDS             8388..11555
                     /locus_tag="LP452_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014423.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11575..12120
                     /locus_tag="LP452_001696"
     CDS             11575..12120
                     /locus_tag="LP452_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBL2014424.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12148..12603
                     /locus_tag="LP452_001697"
     CDS             12148..12603
                     /locus_tag="LP452_001697"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBL2014425.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12600..13358
                     /locus_tag="LP452_001698"
     CDS             12600..13358
                     /locus_tag="LP452_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2014426.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13444..14184)
                     /locus_tag="LP452_001699"
     CDS             complement(13444..14184)
                     /locus_tag="LP452_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2014427.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14245..15498
                     /locus_tag="LP452_001700"
     CDS             14245..15498
                     /locus_tag="LP452_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2014428.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15820..16047
                     /locus_tag="LP452_001701"
     CDS             15820..16047
                     /locus_tag="LP452_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL2014429.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16170..16496
                     /locus_tag="LP452_001702"
     CDS             16170..16496
                     /locus_tag="LP452_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL2014430.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16564..17277
                     /locus_tag="LP452_001703"
     CDS             16564..17277
                     /locus_tag="LP452_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL2014431.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17587..18438
                     /locus_tag="LP452_001704"
     CDS             17587..18438
                     /locus_tag="LP452_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2014432.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18559..20424
                     /locus_tag="LP452_001705"
     CDS             18559..20424
                     /locus_tag="LP452_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2014433.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20472..21881
                     /locus_tag="LP452_001706"
     CDS             20472..21881
                     /locus_tag="LP452_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL2014434.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            21989..22690
                     /locus_tag="LP452_001707"
     CDS             21989..22690
                     /locus_tag="LP452_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2014435.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22849..23328
                     /locus_tag="LP452_001708"
                     /pseudo
     CDS             22849..23328
                     /locus_tag="LP452_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24176..24751
                     /locus_tag="LP452_001709"
     CDS             24176..24751
                     /locus_tag="LP452_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL2014436.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24751..25191
                     /locus_tag="LP452_001710"
     CDS             24751..25191
                     /locus_tag="LP452_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL2014437.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25207..25908
                     /locus_tag="LP452_001711"
     CDS             25207..25908
                     /locus_tag="LP452_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2014438.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         8756 a         4214 c         5355 g         7612 t
ORIGIN      
        1 gactacctgt attagtcctc ttttttgacc agattttgac cagtctattt tacattctaa
       61 tatattcata tgagctaatt ttatgattaa atggtaagtg tttccttgtt tgttttagtt
      121 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      181 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      241 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      301 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      361 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      421 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
      481 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
      541 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
      601 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
      661 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
      721 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
      781 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
      841 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
      901 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
      961 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1021 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1081 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1141 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1201 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1261 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1321 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1381 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1441 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     1501 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     1561 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     1621 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     1681 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     1741 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     1801 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     1861 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     1921 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     1981 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2041 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2101 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2161 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2221 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2281 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2341 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2401 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     2461 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     2521 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     2581 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     2641 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     2701 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     2761 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     2821 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     2881 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     2941 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3001 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3061 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3121 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3181 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3241 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3301 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3361 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3421 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     3481 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     3541 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     3601 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     3661 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     3721 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     3781 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     3841 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     3901 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     3961 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4021 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4081 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4141 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4201 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4261 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4321 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4381 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4441 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     4501 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     4561 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     4621 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     4681 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     4741 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     4801 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     4861 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     4921 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     4981 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5041 gagaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5101 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5161 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5221 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5281 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5341 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5401 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     5461 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     5521 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     5581 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     5641 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     5701 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     5761 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     5821 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     5881 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     5941 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6001 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6061 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6121 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6181 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6241 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6301 gaatattatg tggttaaaat ggtggaaaac caaaacaaag gcaacgacat ggataaatac
     6361 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6421 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     6481 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     6541 ataaaaaata tgaactattt aaatcaataa aactgttcat ctaaatcttt ttattagaac
     6601 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
     6661 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
     6721 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
     6781 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
     6841 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
     6901 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
     6961 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
     7021 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
     7081 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
     7141 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
     7201 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
     7261 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
     7321 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
     7381 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
     7441 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
     7501 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
     7561 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
     7621 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     7681 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
     7741 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     7801 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
     7861 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
     7921 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     7981 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
     8041 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     8101 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
     8161 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
     8221 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
     8281 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
     8341 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
     8401 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     8461 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     8521 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
     8581 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
     8641 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
     8701 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
     8761 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
     8821 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
     8881 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
     8941 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
     9001 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
     9061 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
     9121 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
     9181 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
     9241 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
     9301 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     9361 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     9421 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
     9481 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
     9541 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
     9601 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
     9661 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
     9721 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
     9781 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
     9841 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
     9901 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
     9961 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
    10021 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
    10081 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
    10141 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
    10201 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
    10261 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
    10321 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
    10381 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
    10441 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
    10501 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
    10561 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
    10621 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
    10681 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
    10741 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
    10801 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
    10861 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
    10921 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
    10981 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
    11041 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
    11101 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
    11161 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
    11221 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
    11281 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
    11341 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
    11401 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
    11461 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
    11521 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
    11581 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
    11641 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
    11701 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
    11761 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
    11821 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
    11881 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
    11941 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
    12001 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
    12061 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
    12121 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
    12181 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
    12241 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
    12301 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
    12361 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
    12421 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
    12481 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
    12541 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
    12601 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
    12661 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
    12721 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
    12781 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
    12841 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
    12901 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
    12961 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
    13021 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
    13081 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
    13141 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
    13201 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
    13261 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
    13321 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gatttcaggg
    13381 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
    13441 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
    13501 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
    13561 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
    13621 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
    13681 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
    13741 aggatacagg acgagacttc cacctttttg atcaataaag attttatcga catttacgat
    13801 ctgctgttct ttccaattga tctgttctag cacttgacga atttgttcag ataaattcct
    13861 gtcttttctg atatatccaa tcggaaggat tttccgattg atgatctcta ctgcaaaatt
    13921 cgtataagcg gtgaaaaaaa caatattact atgtgcattt tttttacgta tttgttcggc
    13981 gacatcaata cctgtgagtg attgcttcaa gtcgatatcg ataaagaaaa catcgtgatt
    14041 atcaatagtg cccaactgga tcaactgatt gatttgagag atagaagtga gctgcacgat
    14101 attctcgata tgatgattga ccaattctct gatgatccat tcgctgtgaa actggttatc
    14161 ttctatgata aaatactttt tcatatgcag gctcctatta aaaaaatatg tgtacatttt
    14221 gaaacaaaac aatggaggaa taagatggtc gcaacagtga cactttattt gacctttact
    14281 ttgattttag atttaggtat ctatttattc ttttcgaaac aactgctagt aaaaaacagc
    14341 tttggttgta tagctttgat ggtggtgaca cttttacttc ctatccctaa agtgagcttt
    14401 ccttacctag ttggcttatt attgatgttt cttttttatt ttactacgaa aaacctgttt
    14461 atcagctgtt tgatcagtaa tctttttttt gctatcactg gactcagctt ttttctggtt
    14521 tttgatctga tggattattt aggaaagaga catcttggaa cagcaagttt ttttgcacta
    14581 cttatcgcat ttattttcta tctattagta aaaagtatag acaaacagac gagatttctc
    14641 aacttgcttt ttacttatcc aactgaacac tattttggag gtatttgtat tttattaagc
    14701 tggggcgtga tttacttggt attgggaagt gttcacttcc gctcatctaa ttatctgctt
    14761 ctatctcttt tgtcgatagt catcagtttt ttttctttgt ttttttccat tatgttgatc
    14821 agtttccaag taaaagaaag acaacatcag gaatcgcttc aactgtatca aataaatgaa
    14881 gaatattacc atcatctaga ggaattcaaa catgattata aaagtctact gttttcatta
    14941 aaaattactc ttcaggaaaa taaaaaagaa acgttggaat ttttagaaat attagaagat
    15001 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
    15061 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
    15121 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
    15181 tgtttatcta tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt
    15241 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
    15301 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
    15361 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
    15421 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
    15481 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
    15541 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
    15601 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
    15661 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
    15721 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
    15781 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
    15841 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
    15901 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
    15961 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
    16021 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
    16081 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
    16141 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
    16201 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
    16261 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
    16321 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
    16381 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
    16441 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
    16501 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
    16561 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
    16621 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
    16681 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
    16741 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
    16801 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
    16861 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
    16921 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
    16981 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
    17041 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
    17101 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
    17161 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
    17221 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
    17281 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
    17341 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
    17401 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
    17461 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
    17521 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
    17581 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
    17641 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
    17701 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
    17761 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
    17821 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
    17881 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
    17941 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
    18001 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
    18061 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
    18121 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
    18181 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
    18241 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
    18301 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    18361 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    18421 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    18481 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    18541 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    18601 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    18661 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    18721 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    18781 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    18841 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    18901 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    18961 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    19021 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    19081 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    19141 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    19201 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    19261 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    19321 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    19381 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    19441 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    19501 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    19561 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    19621 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    19681 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    19741 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    19801 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    19861 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    19921 aatcaattag aaaacaaaca tgcgaatgca ggagaaccga ttacttcagc acggcatacc
    19981 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    20041 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    20101 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    20161 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    20221 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    20281 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    20341 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    20401 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    20461 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    20521 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    20581 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    20641 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    20701 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    20761 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    20821 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    20881 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    20941 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    21001 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    21061 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    21121 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    21181 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    21241 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    21301 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    21361 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    21421 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    21481 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    21541 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    21601 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    21661 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    21721 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    21781 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    21841 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    21901 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    21961 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    22021 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    22081 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    22141 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    22201 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    22261 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    22321 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    22381 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    22441 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    22501 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    22561 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    22621 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    22681 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    22741 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    22801 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    22861 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    22921 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    22981 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    23041 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    23101 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    23161 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    23221 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    23281 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    23341 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    23401 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    23461 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    23521 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    23581 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    23641 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    23701 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    23761 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    23821 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca
    23881 aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa tacacgttat
    23941 tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata tcgatgaaaa
    24001 cacgttattt atttttgttt tatctataaa atggctgaaa aatgattttt ttctaaatac
    24061 tatatatagt ggcgtctatc ctatatacca ctaaaaaact acaaaatcta gtcttttatt
    24121 ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt agggagtgtt tttttatgaa
    24181 acttactgtt ttttgcggtt cccgttttgg taataaagaa agttacaaat ttattgcgca
    24241 aacgttagga aaatacatgg cacaagaaga aatcgaactg gtttacggtg gttcatactc
    24301 cgggattatg ggagtgatta gtaaaacagt actggaaaat aacggaaaag tcacaggtat
    24361 ctatccgaat gggctttttg aaactgaatt gcctggaaaa gatgttacta catttattcc
    24421 tacagaaacg atcgatgaaa gaaaggtatt attatttgaa aagggagatg ctgtcctcgt
    24481 tttccctgga ggtctaggta cattggagga attctcacaa cttctttctt ggatagcaat
    24541 cggtctgacg cccgacaagc caatcggaat attgaatatt ggcggttatt acagcggctt
    24601 gcaacaatta ttagaaactt ttgcaaaaga ggattttatg gataaaaaat ggttggatca
    24661 agtaatcttt tccaacaatc cattggaact ggtcaatgaa ttacgagcag tatctattaa
    24721 aaatcagtta ttattgaagg aggcaaaata atgagtcaaa tatatttagc aggaccattt
    24781 ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa aagcgttgga ggaaaacaaa
    24841 acagtcacaa gtttctactc tccacgtcat caccaagaaa gcaattatga actattcagc
    24901 gctggatggg cgcaggaagt atacgaaaaa gatatggaag aactgacaaa tgcagaattt
    24961 gttgttgcta tccttgactt tgagcatcaa acaatcgatc caggaacggc ttacgaatta
    25021 ggtgtggcaa ctatgttgaa aaaaccgatg atcattgtac aagaagagac ggtacctacg
    25081 aacttgatga ttacacaaag tctccatacg tatttaaaat cagaccaagc tgtacgtgaa
    25141 tatgattttg aaacattgcc tgttgaaact tacgtaggcg agtacttata gaagaggaag
    25201 ggttgtatgt cagtgatttt gatttcagga acgattggtg caggtaagag cagtttgacg
    25261 gatatgcttg caaaagagat tgattctaaa ccattttatg aaaatgtcga agataacgaa
    25321 gtattgccgc tcttttattc aaatcctgag cagtacgctt ttttattgca aatcttcttt
    25381 ttgaacaagc gatttttagc aatgaaaaat gctttagtga acgatgacaa tgtgctagac
    25441 cgatccatct atgaagatag cctgttgttc catctaaacg ctgatttagg tcgggtaacg
    25501 gatatcgaag tacagcagta cgatagctta ttagatacga tgctaaacga gttagatgat
    25561 gttgctccaa aaaaacggcc tgatttgatg gtccatatca aagtttcttt ggacacgatg
    25621 ctagaacgta tcaaaaaacg tgggcgtgac tatgaacagc tagaaagcga tgagacgtta
    25681 tatacctatt atgaaacgtt gaacacccgc tataatcaat ggtatgaaga ttttgatgta
    25741 tgtcctaaaa tacaagtgga tggggataaa ttcgattttg tagaaaatcc agaagatgca
    25801 aaaaaggtga tccgtcagat ccaagaaaaa ttagctgaat tggaaggaaa gcctgctgga
    25861 aaatatttta cggataacca gcatgcaaaa ggtcaagaat taggctaaaa ataaaaaacc
    25921 aaacgacggg aaagaaa
//
LOCUS       DAFELO010000039        25817 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFELO010000039
VERSION     DAFELO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.039"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            133..3660
                     /locus_tag="LP452_001712"
     CDS             133..3660
                     /locus_tag="LP452_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL2014439.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LP452_001713"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LP452_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL2014440.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LP452_001714"
     CDS             7448..7789
                     /locus_tag="LP452_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2014441.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LP452_001715"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LP452_001715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL2014442.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LP452_001716"
     CDS             9146..11566
                     /locus_tag="LP452_001716"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL2014443.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LP452_001717"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LP452_001717"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL2014444.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="LP452_001718"
     CDS             13000..13737
                     /locus_tag="LP452_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2014445.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="LP452_001719"
     CDS             13751..14566
                     /locus_tag="LP452_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL2014446.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="LP452_001720"
     CDS             14579..15226
                     /locus_tag="LP452_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2014447.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="LP452_001721"
     CDS             15242..15898
                     /locus_tag="LP452_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2014448.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="LP452_001722"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="LP452_001722"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL2014449.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="LP452_001723"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="LP452_001723"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL2014450.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="LP452_001724"
     CDS             18240..18758
                     /locus_tag="LP452_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2014451.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="LP452_001725"
     CDS             18865..19365
                     /locus_tag="LP452_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2014452.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="LP452_001726"
     CDS             complement(19645..20565)
                     /locus_tag="LP452_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL2014453.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="LP452_001727"
     CDS             20730..21743
                     /locus_tag="LP452_001727"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL2014454.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="LP452_001728"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="LP452_001728"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL2014455.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="LP452_001729"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="LP452_001729"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL2014456.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="LP452_001730"
     CDS             complement(23383..24144)
                     /locus_tag="LP452_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2014457.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="LP452_001731"
     CDS             24333..25427
                     /locus_tag="LP452_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2014458.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25817)
                     /locus_tag="LP452_001732"
     CDS             complement(25513..>25817)
                     /locus_tag="LP452_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2014459.1"
                     /translation="ITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8543 a         4475 c         5441 g         7358 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatag
//
LOCUS       DAFELO010000040        25695 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFELO010000040
VERSION     DAFELO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.040"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(153..587)
                     /locus_tag="LP452_001733"
     CDS             complement(153..587)
                     /locus_tag="LP452_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014460.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(697..2556)
                     /locus_tag="LP452_001734"
     CDS             complement(697..2556)
                     /locus_tag="LP452_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2014461.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(2674..3833)
                     /locus_tag="LP452_001735"
                     /pseudo
     CDS             complement(2674..3833)
                     /locus_tag="LP452_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(4140..5564)
                     /gene="araA"
                     /locus_tag="LP452_001736"
     CDS             complement(4140..5564)
                     /gene="araA"
                     /locus_tag="LP452_001736"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL2014462.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(5580..6278)
                     /locus_tag="LP452_001737"
     CDS             complement(5580..6278)
                     /locus_tag="LP452_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL2014463.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(6235..7896)
                     /locus_tag="LP452_001738"
     CDS             complement(6235..7896)
                     /locus_tag="LP452_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL2014464.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            8114..9514
                     /locus_tag="LP452_001739"
     CDS             8114..9514
                     /locus_tag="LP452_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL2014465.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(9766..10845)
                     /locus_tag="LP452_001740"
     CDS             complement(9766..10845)
                     /locus_tag="LP452_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2014466.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10931..11890)
                     /locus_tag="LP452_001741"
     CDS             complement(10931..11890)
                     /locus_tag="LP452_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL2014467.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(11902..13413)
                     /locus_tag="LP452_001742"
     CDS             complement(11902..13413)
                     /locus_tag="LP452_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL2014468.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(13435..13998)
                     /locus_tag="LP452_001743"
     CDS             complement(13435..13998)
                     /locus_tag="LP452_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL2014469.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(14004..14867)
                     /locus_tag="LP452_001744"
     CDS             complement(14004..14867)
                     /locus_tag="LP452_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2014470.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(14881..15777)
                     /locus_tag="LP452_001745"
     CDS             complement(14881..15777)
                     /locus_tag="LP452_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2014471.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(15952..17241)
                     /locus_tag="LP452_001746"
     CDS             complement(15952..17241)
                     /locus_tag="LP452_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2014472.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(17213..18730)
                     /locus_tag="LP452_001747"
     CDS             complement(17213..18730)
                     /locus_tag="LP452_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL2014473.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            18928..19818
                     /locus_tag="LP452_001748"
     CDS             18928..19818
                     /locus_tag="LP452_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL2014474.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(19891..20715)
                     /gene="nadE"
                     /locus_tag="LP452_001749"
     CDS             complement(19891..20715)
                     /gene="nadE"
                     /locus_tag="LP452_001749"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL2014475.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(20727..22199)
                     /locus_tag="LP452_001750"
     CDS             complement(20727..22199)
                     /locus_tag="LP452_001750"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL2014476.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(22836..23198)
                     /locus_tag="LP452_001751"
     CDS             complement(22836..23198)
                     /locus_tag="LP452_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL2014477.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            23421..24173
                     /locus_tag="LP452_001752"
     CDS             23421..24173
                     /locus_tag="LP452_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2014478.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(24313..25236)
                     /locus_tag="LP452_001753"
     CDS             complement(24313..25236)
                     /locus_tag="LP452_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2014479.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(25251..25523)
                     /locus_tag="LP452_001754"
     CDS             complement(25251..25523)
                     /locus_tag="LP452_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014480.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         7508 a         5377 c         4334 g         8476 t
ORIGIN      
        1 aaaaggagta tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc
       61 attatcataa aggattttga tcactctatc ataaaggtaa tattacattt atattagata
      121 actctagttt ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt
      181 ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg
      241 aatagatctt catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct
      301 gctacatatg aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt
      361 ggacgcaaag tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat
      421 aacaggatga tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt
      481 gcgtttctga tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg
      541 acgatttgat cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta
      601 ctttctccat tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt
      661 gaaaaaattt aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc
      721 tgctaaacta tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta
      781 aatctcttgt tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta
      841 cagttctcgt tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc
      901 atttgttgtc tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat
      961 tgcatcgtga gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt
     1021 ttcatgcaat gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag
     1081 atgatccccc caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa
     1141 ctgcttcagt gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt
     1201 cccacttcca tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg
     1261 catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag
     1321 tgtcattact tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc
     1381 cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc
     1441 ctctctttta tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc
     1501 gtttaacgct gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg
     1561 ggtggtgagt tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat
     1621 atttgctgtt ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc
     1681 tacttcataa taatctgcca atggctcacc agatacttct acgccgttta atcgactagg
     1741 atctgagaaa ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt
     1801 atttttttca tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga
     1861 atatccttca ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa
     1921 accagtgtta tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata
     1981 tgcttttcgc aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat
     2041 tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg
     2101 aaacatgtac cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa
     2161 cagcatagtg ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc
     2221 tggataaatc ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc
     2281 gatggctacg acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa
     2341 aataagaata atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa
     2401 agcaaatttc aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt
     2461 actaaaaaca acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt
     2521 ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca
     2581 ataaaagaat gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa
     2641 gaagaattaa tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa
     2701 gcggaccttt cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc
     2761 aaaatcagta ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga
     2821 tatatctgcg atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt
     2881 ggttttcttt tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga
     2941 tggataattg accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac
     3001 tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata
     3061 agtatacggt cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga
     3121 tgttgttttt ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca
     3181 ttgataagta cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt
     3241 gtatcttctg tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct
     3301 aaatctgctt gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc
     3361 atccctaagc tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga
     3421 ccgatggttt tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt
     3481 tctaaaatat taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg
     3541 gaagataaga gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt
     3601 gtattcatga cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca
     3661 acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta
     3721 ctacgaataa ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt
     3781 aacttttcta tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc
     3841 cctccttact aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact
     3901 tcctagaagc aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg
     3961 acaagttact gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga
     4021 taaatggtgt tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag
     4081 caaatttcgt caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt
     4141 tatctaataa ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt
     4201 gttagattta gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca
     4261 ccttcatgga aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct
     4321 tcagtcgttg gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa
     4381 tctgccatcg atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga
     4441 tcttctctat ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc
     4501 gtcggatcta cttccatcat atgggattcc aagattgctt cttggccact agctagctcg
     4561 taagtataat cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga
     4621 tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa
     4681 cgttgtacag ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta
     4741 aaagcgttat agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt
     4801 acttttacat gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat
     4861 tccttgtact cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt
     4921 acaagatctc cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact
     4981 ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt
     5041 ccaaaacttt cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc
     5101 tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg
     5161 aacccatatt cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc
     5221 gttttccaag gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga
     5281 agctttgtcc cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca
     5341 cctgctactt cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta
     5401 actgctaatt cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg
     5461 accatttctt gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt
     5521 gatcctacaa caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt
     5581 tatttgtttt gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa
     5641 atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg
     5701 catacttcat ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta
     5761 aatggaccgt gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct
     5821 ttgaatgttt ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt
     5881 tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc
     5941 atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg
     6001 acgattcctc cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa
     6061 gagggattga gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct
     6121 gctgtcatct cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga
     6181 tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga
     6241 tttgcttggt atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg
     6301 caattgatga atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat
     6361 ctgatagcct gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa
     6421 cacgtcttct gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc
     6481 taataacgcg attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc
     6541 ctccatagca gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg
     6601 ccccactaaa cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat
     6661 tgcaccaaaa gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc
     6721 tggtgtacgt acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa
     6781 ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa
     6841 taccgtgaat aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa
     6901 aatactgacc caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag
     6961 ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc
     7021 taatacgatc atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac
     7081 actgttcgta gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc
     7141 tggtctcaaa tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc
     7201 ttcttctcct gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc
     7261 tttttcgatt aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg
     7321 atcgatagga aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg
     7381 ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt
     7441 attcaaaata gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa
     7501 tttttccgtt aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg
     7561 tactagtaac tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc
     7621 aatcgacccg attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt
     7681 tcggtaactg acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt
     7741 ttctagtttg ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat
     7801 caatacagct ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat
     7861 gacatctaac atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg
     7921 tcaaacctga tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta
     7981 cgtacatgtc aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca
     8041 taagttataa aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa
     8101 ggagagaata cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg
     8161 cgcatttggc ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt
     8221 tttacagcat gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc
     8281 ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg
     8341 acgacgaaaa atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc
     8401 atctgcccct cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc
     8461 tgttggcgct gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact
     8521 aaggggaaga ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta
     8581 tgttgcagat tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg
     8641 gctcgctgct gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc
     8701 gcgttttctt atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg
     8761 gacaccaaaa gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa
     8821 gacaaccgga acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc
     8881 cggtattggt gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat
     8941 tccgctgatc gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat
     9001 catccaagga atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt
     9061 caatcggcga actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc
     9121 agctgtttta ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt
     9181 atgtatttat gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg
     9241 cgaaatattc ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg
     9301 gattggttct ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga
     9361 aatcgttttc ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat
     9421 cgtaccagaa actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac
     9481 ttatcctaaa cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct
     9541 tcaagaaata agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc
     9601 ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg
     9661 gagaggtggt gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc
     9721 gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc
     9781 tttcactgtt tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc
     9841 tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg
     9901 cgtcaatgtt gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac
     9961 gatcgatagg tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact
    10021 agctatttga tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg
    10081 tactgttttt tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt
    10141 caaaaaacgc acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc
    10201 ttgaagatcg tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata
    10261 ttctgctgcc ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc
    10321 ttcataagca gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag
    10381 tgctaagttt gggttatact gattactttt cgtaggctca acgatcaaac catctacccc
    10441 ataagcaatc attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc
    10501 taaaagcaat gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg
    10561 aaaaatataa tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg
    10621 tgttttttct ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag
    10681 atagccctca tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata
    10741 tatttcttgc agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag
    10801 gatcttttgt tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt
    10861 ttatttgtac gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg
    10921 tttcttgtgc tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat
    10981 tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt
    11041 ggttcttctc tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg
    11101 taaacgagtt gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct
    11161 ccgataattt atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca
    11221 ggcccatggc atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat
    11281 tgcgagtaag gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact
    11341 caggtataga aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata
    11401 gataaagatt agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct
    11461 ttccttgaga agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt
    11521 gtcccttttc tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca
    11581 tgcggtgatg atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat
    11641 accattttcc attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt
    11701 cttcatgagc ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg
    11761 cccttcttaa ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat
    11821 ctgtatgttt atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg
    11881 tcttcatcat aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt
    11941 ttctatccct acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt
    12001 tattttttct ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta
    12061 gtgctctcct tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt
    12121 tgtgcgattg atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac
    12181 ataaggaaca ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa
    12241 tacttgacct ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca
    12301 tgcttgtcct ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca
    12361 actaatcttt acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa
    12421 tgtatcttcc atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg
    12481 tcttttttcc ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc
    12541 acttctcaaa tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc
    12601 gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt
    12661 agtcaagtct tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt
    12721 ctcataagct tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt
    12781 agagcttcca actaagattg tttcaattga atcatccact aacttcatga cttttgaggc
    12841 ttcatttgct aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc
    12901 tagctcattc cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat
    12961 tcttaaatca ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc
    13021 ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact
    13081 gatccattta aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa
    13141 atcaattttt tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga
    13201 tgtaaagttt cctccaggat agcgaatgac acctaattct aattccttta cataagagag
    13261 gacatcttgt ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt
    13321 atagacgaca cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc
    13381 taccgtaaaa tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct
    13441 ttcatttcct atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa
    13501 aataaaagga gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag
    13561 aatatttctc aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa
    13621 tgtgatatct ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat
    13681 aagtaaaact acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt
    13741 aacaagaaaa taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg
    13801 ctttattcct tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc
    13861 cattacataa aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat
    13921 tacccagaga atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt
    13981 agtctcaact attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca
    14041 aaatatttct gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca
    14101 gccattacag gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt
    14161 aaagttctca tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta
    14221 ttcatcagcg tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt
    14281 ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct
    14341 ggtaaagtaa taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga
    14401 atgatcaaac ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga
    14461 atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat
    14521 tttcctttga aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa
    14581 gtgatcactg tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc
    14641 gttgtccaaa ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga
    14701 aatgagataa tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac
    14761 gcaataacca acaatataac tagcatacta atcgttcgaa tgattttttt accactaact
    14821 gcttttcgac tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc
    14881 ctatttcatt tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc
    14941 aacaatgata aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt
    15001 aaatcctcga ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc
    15061 ggttgcaaca tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca
    15121 aaaacctaag gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt
    15181 tgcaccatcc atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa
    15241 gagaatcgtc atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat
    15301 agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta
    15361 aggaatcgcc cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa
    15421 tgaacaaata actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt
    15481 agataatgct actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt
    15541 gaaatataaa gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa
    15601 attttcaaaa cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat
    15661 agcaaatgtt cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt
    15721 taaaaacaaa tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata
    15781 gtccacctca tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa
    15841 gatatccgaa caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat
    15901 cattcagata tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg
    15961 cagtaattga ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc
    16021 taataatttt tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa
    16081 acctggtaaa tatggctctg catagtccat ttgtttcgat aattcagata caataggatc
    16141 attttttact tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat
    16201 actccatttt ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc
    16261 ttttttatta ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt
    16321 ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc
    16381 tttcaatccg ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat
    16441 taaattgtcc atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat
    16501 tttttggatc gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata
    16561 atcatcgttc actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc
    16621 atccttattg aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat
    16681 ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa
    16741 caaatcatta ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt
    16801 accttctact tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc
    16861 atattcccag aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc
    16921 tggataattt aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt
    16981 ttcattcaaa ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt
    17041 tttattaaat tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt
    17101 ccaaaaagtc aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca
    17161 agctcctaaa atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt
    17221 cattttttgt tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt
    17281 tcaataaaca attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta
    17341 cttgttcact tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat
    17401 caatcactcc ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc
    17461 cacggttaac tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat
    17521 acggcacttc ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca
    17581 cctctccttg tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag
    17641 cttctccgtt tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag
    17701 caatctttac tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg
    17761 catcttcaaa attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt
    17821 gctcatcttg ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct
    17881 gcttcttact atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga
    17941 accgatccat gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat
    18001 aatattgatg cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact
    18061 cccaatcacc aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg
    18121 aatcatctac gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg
    18181 ctgttttatg tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa
    18241 ttccaaaagg gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag
    18301 gaaaattaca atattcgact aaattacgag ctgcatctac tccacgagta cccagattga
    18361 cagccatatt gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa
    18421 cttcattcgt ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg
    18481 gcccaatact gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca
    18541 tgggaatgtt caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag
    18601 ctgatggatg atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa
    18661 atgaactgta caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg
    18721 tggcttccat aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt
    18781 tgtcaataat ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat
    18841 taataatgat taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata
    18901 agaaaaacct tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg
    18961 gtatatgaag cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa
    19021 aagatgaatg tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg
    19081 cacgtgaaaa agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa
    19141 atttcgagcc ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct
    19201 tttgatacga aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca
    19261 acatgcggtt tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt
    19321 atggatcctc gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat
    19381 caggcaccta attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa
    19441 atttcttctg aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta
    19501 aatggtgtgg agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa
    19561 tatacgcctg attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga
    19621 attaccaaac acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac
    19681 atccctaaag agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat
    19741 gtggggggct gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta
    19801 aaagtttact acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc
    19861 tatgaaggga cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat
    19921 tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg
    19981 agcgggaact tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc
    20041 gacttcatct gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta
    20101 taatttttcc ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg
    20161 gaataatggc aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt
    20221 ttctgcagca tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg
    20281 cgtaatcata cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc
    20341 tgcttcgctt aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact
    20401 aacatcgggc tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc
    20461 ataaggaaga cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc
    20521 ttccatcgtt agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc
    20581 tagtacaaaa gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact
    20641 tttacgtatt tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc
    20701 tgtctgcagg ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt
    20761 ttcattcgtt ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg
    20821 tatttttgcg gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt
    20881 gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag
    20941 atatcttgta aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc
    21001 actggatgga acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg
    21061 tagtctcctt cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct
    21121 ggagtagtta ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca
    21181 tcatcttcga tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg
    21241 atcaattttg tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa
    21301 atcgttgctt catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca
    21361 tctaattgtt cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg
    21421 ccttggaagt tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct
    21481 gatttcaacg tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag
    21541 gccataaatg cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg
    21601 ctagctggta tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg
    21661 atatatgccg ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca
    21721 aattccaata gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc
    21781 aacgtttgga aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa
    21841 ggcccttcca cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa
    21901 cgaactgttg ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact
    21961 tctaatccac gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg
    22021 actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag
    22081 taacattcaa aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata
    22141 ttgatttgat atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc
    22201 ttgtattact cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata
    22261 ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa
    22321 aaatgaactc catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag
    22381 aatacttatt ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt
    22441 acttgataaa ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg
    22501 aacaaatata accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa
    22561 tacgtctttc cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg
    22621 aatttttttg gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa
    22681 aaaatatcac acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa
    22741 acaaaaatgg cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg
    22801 gagcaggtta ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt
    22861 tgttctatca ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc
    22921 tcctgtttca ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga
    22981 atcgcatagt cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc
    23041 tttttatcat atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa
    23101 taattaatag ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata
    23161 tttaaattac tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag
    23221 tcgatttaga tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta
    23281 caattgaacc tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt
    23341 tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac
    23401 atttgtcagg aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag
    23461 tcagtccgag aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca
    23521 attcttcagg ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat
    23581 tttatcgaac aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt
    23641 ttgaccacca gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa
    23701 tcgataccct cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa
    23761 tgagtgacaa agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat
    23821 cctatggaca agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa
    23881 aatggatgaa tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt
    23941 tatttgatct cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct
    24001 tccaagctca taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata
    24061 aaggactagg aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa
    24121 gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta
    24181 catgatccaa caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag
    24241 ttagattttt atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta
    24301 ctttaaatat attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc
    24361 tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg
    24421 gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc
    24481 ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc
    24541 aactccaagc tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc
    24601 ccaaaaaaca agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat
    24661 ttttgagtta taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc
    24721 ttgctgcttc aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca
    24781 gcttgcctgt tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc
    24841 caataaatca tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc
    24901 tgcaaattct tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt
    24961 cactttgtaa tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata
    25021 cggatgattg gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg
    25081 tctggcattt gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct
    25141 tttttcctca tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata
    25201 acctgtgagc tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa
    25261 agattttctt gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt
    25321 ccttcatgcg aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg
    25381 atttcattca ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac
    25441 gttaatacgg atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct
    25501 gttatctcta cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat
    25561 gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat
    25621 tattaccaaa gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt
    25681 tattattagt gaatg
//
LOCUS       DAFELO010000041        23570 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFELO010000041
VERSION     DAFELO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.041"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="LP452_001755"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="LP452_001755"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL2014481.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="LP452_001756"
     CDS             complement(1171..1866)
                     /locus_tag="LP452_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL2014482.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="LP452_001757"
     CDS             complement(1879..2595)
                     /locus_tag="LP452_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL2014483.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3530
                     /locus_tag="LP452_001758"
     CDS             2925..3530
                     /locus_tag="LP452_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL2014484.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3855..4178
                     /locus_tag="LP452_001759"
     CDS             3855..4178
                     /locus_tag="LP452_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL2014485.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4360..5730
                     /locus_tag="LP452_001760"
     CDS             4360..5730
                     /locus_tag="LP452_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL2014486.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5860..7161)
                     /locus_tag="LP452_001761"
     CDS             complement(5860..7161)
                     /locus_tag="LP452_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL2014487.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7391..9349)
                     /locus_tag="LP452_001762"
     CDS             complement(7391..9349)
                     /locus_tag="LP452_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL2014488.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9526..9792)
                     /locus_tag="LP452_001763"
     CDS             complement(9526..9792)
                     /locus_tag="LP452_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2014489.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10302..12428)
                     /gene="pnp"
                     /locus_tag="LP452_001764"
     CDS             complement(10302..12428)
                     /gene="pnp"
                     /locus_tag="LP452_001764"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL2014490.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12616..12885)
                     /gene="rpsO"
                     /locus_tag="LP452_001765"
     CDS             complement(12616..12885)
                     /gene="rpsO"
                     /locus_tag="LP452_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL2014491.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13136..13699
                     /locus_tag="LP452_001766"
     CDS             13136..13699
                     /locus_tag="LP452_001766"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL2014492.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13952..14473)
                     /locus_tag="LP452_001767"
     CDS             complement(13952..14473)
                     /locus_tag="LP452_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL2014493.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14476..15318)
                     /locus_tag="LP452_001768"
     CDS             complement(14476..15318)
                     /locus_tag="LP452_001768"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL2014494.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15311..16036)
                     /locus_tag="LP452_001769"
     CDS             complement(15311..16036)
                     /locus_tag="LP452_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL2014495.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16212..16508
                     /locus_tag="LP452_001770"
     CDS             16212..16508
                     /locus_tag="LP452_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014496.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17076..18668
                     /locus_tag="LP452_001771"
     CDS             17076..18668
                     /locus_tag="LP452_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2014497.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18681..19421
                     /locus_tag="LP452_001772"
     CDS             18681..19421
                     /locus_tag="LP452_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2014498.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19589..20938
                     /locus_tag="LP452_001773"
     CDS             19589..20938
                     /locus_tag="LP452_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2014499.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21066..21656)
                     /locus_tag="LP452_001774"
     CDS             complement(21066..21656)
                     /locus_tag="LP452_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL2014500.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21803..23155
                     /gene="brnQ"
                     /locus_tag="LP452_001775"
     CDS             21803..23155
                     /gene="brnQ"
                     /locus_tag="LP452_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL2014501.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6749 a         4747 c         4490 g         7584 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcgaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaaa
     3481 gcacaggtga aggtggcgaa ccagaagtga tcggttcaac agaagaataa tactgaaaca
     3541 atcaggtggt gttagaagcg gcgggtcagc agcttttaac acgccttttt tatttctaat
     3601 cgtttatttt catatggggc ttccactttt gtaaatggct ttacaaacga aaaatcctaa
     3661 tttaccaagc ttttcctcgc atacttttat cgtaccttta taaaaattta tactttgaaa
     3721 aaaagtattc aattattgtt gcatttttcc tgattcttta ttagaatgaa ccctcgttga
     3781 agtaagtatt tctagtttat cctaggatta attataaaaa taaaacattc gttgattcga
     3841 aggaggttaa acagatgttt gaaagttttg atagcagccg caaccgttac gcttcagttg
     3901 gtgtcgtatc aagtttgcca gatgaattga ttgacagtat atggtttatt attgacctag
     3961 atcttaaggg agtcattcca ttagaaaata tattaacctt tgatttgatc gaaaatcgtg
     4021 gaaaagtgac gatgcatttt tctcaagcag gaagcaatgt ggaaatggat attgatttgc
     4081 cgtttgccta ttcgaaccaa tttcctgctc aagtatacgc ctatgatgac ggtacgagag
     4141 aaacgatcct tttaccaagt gagattagac aatattaagg tgatagataa acgtggatgt
     4201 cccttatttt acagaccagg ccaagttgtt gattctttga ctcaattctt ggcttctttt
     4261 tatggagttt cataaattat cattgatttc taattggtat tctcaggata aacgctatat
     4321 actactaact aaattttaaa gatataagga gtttaaaaga tgcgcgattt aacaaaagga
     4381 agccccgcta aactgatttt gatgttcact gttcctttac tgatcggaaa tgtttttcag
     4441 cagttctaca atatgatcga tatgatcatc gtgggtcaga cgctaggtaa aaatgcgctg
     4501 gcagcggttg gtgcaactgg aagcctgaca tttttgatca ttggctttgc ccaaggttta
     4561 acagctggtt tagccattat taccgcacaa cgttacggtg caaaagatta tcggggtctg
     4621 aagaaaagtt ttgctgctag tgttgtcatt agtcttgtcg tcactgtgat tttaactgtc
     4681 ctaagtctcc tgtttattcg tccgatgctt caattgatgc aaactcctcc tgaaatactt
     4741 gatcaagcac aaacatttat ttcaattatt ttattaggga tctttgcatc agtcagtttc
     4801 aatttgcttt ctaatgtcat tcgcgcactt ggtgacagcc gcacaccact attctttctg
     4861 atcattgcgg tcatcatcaa cgtcgtactt gatttgatct ttattatcta ttttggcatg
     4921 ggtgtggaag gtgcagctat tgctacagta atcgcacaag tttcttccag cgtactgtgt
     4981 ctagtgtata tcaagaaaaa aatgcctttg ttacagttac gcaaaaaaga tttcaaattt
     5041 gaaaaagaag agtttgctgt acacctaaat gctgctttgc caatggcgtt tcaatcttcg
     5101 atcatcgctg ttggtgctat cgtgttacaa gcagcactaa atagtctagg gacagatgtc
     5161 gtagctgctc aagccgctgc tggtcgtatc gaccaatttg caaatcagcc gatgatgtca
     5221 tttggtattg caatggcaac tttcactgca caaaactatg gtgcgaaaga atatggccga
     5281 atcttgcaag gggtacgtca aactttactg atgagtgtcg gatttagtct cttagcaggt
     5341 gctatcgtca ttttcttcgg tcatttcttt gttcaattgt ttgttagccc atcagaaaca
     5401 cgtgtatttg aattagcaca aacctacttc aatatcaatg ggtctcttta ctggatctta
     5461 gcagtcctgt tcattttacg ctacacactt caagggcttg gacaaagcaa gatccctacg
     5521 atcgccggta tgatggaatt gcttatgcgt tcttttgcag ctatcgttct tacaggaatg
     5581 tggggatacc caggagcagc agcagcttct cctttagctt ggatcggttc tgttgccgtg
     5641 ttgctttact cttatatgcg ttcgatgaaa caattgaaac acatgggtga agaggaacat
     5701 ttcacattag aacatgcaga agcaagatag ccttgattaa aagaaatcgt tgcggctaaa
     5761 aatgaaaact tttattgaaa atatcttctt cgtttaccga aaacaggttt tttagataac
     5821 aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt tattctattt ttctttccgt
     5881 ggactctact ttgaaataat cgatatgtgc atgctggtat tctaataccg tgaaacggaa
     5941 tgatgcaatc tcaatgattg ttcctgcttt tatttcttta tatcggctag aagcaaagcc
     6001 cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc tctacatgaa aataacgctg
     6061 gaaatcatct aatgttagtt tgccggagat gatataagag ccatctgact gttccgctat
     6121 caatgcttcg tccaaatctg tctcatctcg gatttccccg acgatttctt ctaaaatatc
     6181 ttcaatcgtc actaagccgc ttgtccctcc gtattcatct tttaagatag caatatgctt
     6241 tccctttttt cgcatgatcg tcaataattt ttttataggg atcgtttcag ttgttacgat
     6301 cggctcttca atcaactgtt ctactgtcag ctggtcatct ttataagatt gcttgatcaa
     6361 atctggcaag gtgacataac ctaaaacatt gtcttttgat tctaagataa ccggatagcg
     6421 agtatgtcct tgttcaatcg aagcttttat cgcttctttg acggtttctg aagcttcgaa
     6481 tacttgcatg gacgtacggt ctacttgtat atcttttgcc aaggtatcat caaattcaaa
     6541 cacgttttcc aaataatgat attcttcttt ggtcaactct cctttttcat aagaattcaa
     6601 tgcgactaat gtcagctctt cttgagacaa tgtctcttct ccttcagata cgagacgcac
     6661 acctaatagc ttccctagcc ctgcagctga atgattcaat acccaaataa aaggaaacat
     6721 tactttataa aaataatgaa gtggtttaac tacgaacaag acgaccttct ccgtattgac
     6781 gatactatag ctcttaggaa gcagttcgcc aataaccaca tcgatatacg taactaaaat
     6841 aaatcccagc gccacagaga ttgtactgct aatagccgaa ctaaaaggta gtttaccgat
     6901 cactggttct agtaaagccg cgacagatcc ttcaccgacc cacccgatga tcaaaccggt
     6961 tagcgtaatt cctaattgac tagctgataa atagttatct aaatggtgtg taacatgaag
     7021 agctagcttg gcgttcttta cattttcctt ttctagttgt tccaatcttg atcgtctgat
     7081 tttcactaaa gcaaattcac ttgctacaaa aaatgaagta attgcaatca tcaaaaataa
     7141 aataatgaaa ttgataacca tattctcccg cctttctctt tttctattgt accctttttc
     7201 tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa taaaaaagga gatgcggcat
     7261 ttttcagcca catctcctta tcacttcaaa atcattgcgt tacttgttgc taaacagctt
     7321 tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag ttcaggtcaa
     7381 agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca atgataagcg
     7441 gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct ggcgaggtag
     7501 cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt cctagagttg
     7561 tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact tctcgccctg
     7621 gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc aaatcatgga
     7681 tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag aacagattca
     7741 cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta tatggctcat
     7801 ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt ttatctaaaa
     7861 tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc aagaagacca
     7921 catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc tgcgggatat
     7981 aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct tgttcccaaa
     8041 taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg cctcggaaga
     8101 atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa aaggtaacta
     8161 aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc agtgctgttg
     8221 tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga tgagacgttc
     8281 ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact gctgggatat
     8341 acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc agtttcaact
     8401 tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa gcaatgactg
     8461 cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc atttggaaga
     8521 atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc aacaaccacg
     8581 cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag ctcagacgaa
     8641 tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct gtcgtagcca
     8701 ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa agcagctgga
     8761 tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag ttcattaatc
     8821 ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct tttccgacaa
     8881 tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa gtgataatga
     8941 cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct cgcaagcctt
     9001 caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt aaggcagctc
     9061 ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt gtgtagagtg
     9121 agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca tatttgattg
     9181 ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca aaatcaggat
     9241 tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa agcggacttg
     9301 tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt gatatctttt
     9361 tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga tatcctttag
     9421 gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta aattgcttct
     9481 aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt ctttctttgg
     9541 acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa ttgcacccaa
     9601 taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta gtaaaaattc
     9661 gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc ccgggacttc
     9721 ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata acgaccataa
     9781 aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt ttacgcttta
     9841 ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag ttgtaacaaa
     9901 agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag aggttgtgaa
     9961 atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    10021 cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg agaggttgta
    10081 aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc ctaatgtttt
    10141 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    10201 aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat tgtccccctg
    10261 tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc ttcatttttc
    10321 gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc ttgtttatcg
    10381 atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc gactttattg
    10441 acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt gatcaagttg
    10501 acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc accaactttg
    10561 acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc tgcatcagct
    10621 gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt ttcatcgatg
    10681 attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc aggttcgatc
    10741 tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc agcaatcgtt
    10801 gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa tgcttcagtt
    10861 aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt gataccatct
    10921 tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc ttggatatca
    10981 gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc ggcgactggc
    11041 gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat acttgcttgg
    11101 gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata aggaaattct
    11161 tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg tccgatttcc
    11221 cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg gaaattgtag
    11281 tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg gtgctctcct
    11341 agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa caaacctgat
    11401 ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc atccaattta
    11461 cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc tttttcaaga
    11521 tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg acctgcaaat
    11581 ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc ttcgcgagca
    11641 agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc aaagatttct
    11701 tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt ccctactgct
    11761 tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc aaacagcaat
    11821 gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat attgatggct
    11881 tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc tactgttgga
    11941 ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg gccgtcaaat
    12001 gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat agctggcgag
    12061 cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg gaagccttct
    12121 gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc ttccgtactt
    12181 ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata cattttttct
    12241 tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga ggccacagct
    12301 gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc ttgttttgct
    12361 agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa tacttgtttt
    12421 tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa caaatgaaaa
    12481 ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca tctagcttga
    12541 tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg tgaatcccac
    12601 tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc ggtaacgttg
    12661 aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt ttttcattag
    12721 gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca agtggttgat
    12781 ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt catggcgagc
    12841 gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt tcacctcttt
    12901 cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag tggcaggtca
    12961 tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca agtagttaat
    13021 cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt tgtggtaaac
    13081 tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat attccatgat
    13141 tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg caaaagaagt
    13201 ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg agttcttaaa
    13261 aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg ttggcttagc
    13321 tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa gtcctgaccc
    13381 agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt tgagccattc
    13441 tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc gagaagtacc
    13501 aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga atggtgaaaa
    13561 acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa tcgaccatat
    13621 caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac taaaagaagg
    13681 cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta caagaaagct
    13741 ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc tccactaatc
    13801 gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat tggatacgca
    13861 tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga tgatagaata
    13921 gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt cctgcttcag
    13981 ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc gtatgcagat
    14041 ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa cgtctcataa
    14101 aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca ccgtaaaggg
    14161 gatgttccac aattacatca atgatttcac caccattttc aacgattagt ctcagttctt
    14221 cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca gtattggttt
    14281 cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct gcagctcgta
    14341 gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc gcaaacttgc
    14401 tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt cgccgtgtct
    14461 ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg agtgtttcat
    14521 caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca gcagactaac
    14581 aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt tttcttcttt
    14641 tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag caaccaactc
    14701 tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg ttagttcttc
    14761 ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt cgggaaaaga
    14821 tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga cacccggcgt
    14881 gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt ttgcgtactc
    14941 cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa gctggacatt
    15001 cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa atgtacaatt
    15061 gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa aattgataac
    15121 tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca attgggatcg
    15181 cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa acatacggtc
    15241 ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt tttctttttc
    15301 ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa acaaccgctc
    15361 cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga ttgattacta
    15421 agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga acttgaaaac
    15481 tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc tgctccatga
    15541 ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt cctccgcatg
    15601 cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga cattttcgac
    15661 aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta gcctttgcgt
    15721 gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct agatgtgcta
    15781 tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg acaaattgat
    15841 agaatttatc cggttcattc accattgctt gatgactcag caagtattcc ggctgctcca
    15901 tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt gtggataccc
    15961 ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct gcttcgtcta
    16021 ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac tcgaacaaat
    16081 ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt tgagcaattt
    16141 atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt ataaaagtga
    16201 ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct tgtatcgcct
    16261 ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa aatggaaatc
    16321 tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt acattagccg
    16381 tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat ctaatattag
    16441 ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca aagaaagcgg
    16501 cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag ttcttttttt
    16561 attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga tagatgattc
    16621 acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt attttttcaa
    16681 aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt ttgtataaat
    16741 aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat taaccctgtg
    16801 gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg cacttttttg
    16861 cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc agtgatgcta
    16921 agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg cctttttata
    16981 tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt catctagacc
    17041 gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg gtttcagttg
    17101 ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca aatgcagatg
    17161 aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca cccttcaact
    17221 ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat agttacgccg
    17281 gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa aaattagtca
    17341 tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag atcgacgcga
    17401 tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt accgatcctt
    17461 attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca aaagcaaaaa
    17521 gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc cactatactg
    17581 tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc tcagcaatga
    17641 gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg gaaggtgtaa
    17701 cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca gatggcttcc
    17761 agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac ccggatatcc
    17821 aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa attatggacc
    17881 aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc atcatttctg
    17941 atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct ggacttacct
    18001 tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg attggtgtct
    18061 tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa atcggaaatg
    18121 cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta caagcaatgg
    18181 tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg attgccgctt
    18241 tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt ggggggattt
    18301 ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc catggccaaa
    18361 cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca acaggaaatg
    18421 agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta gctgatttgt
    18481 tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca tttacgatcg
    18541 tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg gcggtagaaa
    18601 agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat tcaaatttgc
    18661 aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat ctaagaaaaa
    18721 gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa ggtgaagttg
    18781 tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt atcaatttac
    18841 tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta gctcctaaat
    18901 ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc aacctgttca
    18961 acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg aaacgagata
    19021 aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg gatcgttata
    19081 tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca attgctcggg
    19141 cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg ttagaccctg
    19201 aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc ttgacaatgc
    19261 ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc atttttatgg
    19321 ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat ccgaaagaag
    19381 aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag ctgattggga
    19441 caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa aggtaaattt
    19501 ctgaaaattt cggaatctta gttgtgtttt tcagaaaact ctcttaatat ggaagtaatt
    19561 aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc agcgtggact
    19621 gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg ggttgttttt
    19681 aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct atcttattac
    19741 tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat caaacaccaa
    19801 taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg ctttcatcac
    19861 tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa cagccatcgg
    19921 aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac tgattgcatt
    19981 aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt tagagttttg
    20041 gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag cttatatgat
    20101 cctcacccac tatacgacag atattggcac agccagtatc acgaatctat ggtctcatgg
    20161 cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg taacctttgc
    20221 ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc ctgaaaaagt
    20281 gttgccggaa gctatcaata atattcctat caggatcatc ttattttact taggatctct
    20341 ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca gtccatttgt
    20401 agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt tagtcgtatt
    20461 aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta tgcttcgttc
    20521 tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac atcatgtgcc
    20581 agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct tgaattacgt
    20641 gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt tcttgtttat
    20701 ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg gcaaaaaagc
    20761 tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc tttacatggc
    20821 ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat tactaacacc
    20881 tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta agaagtaaga
    20941 aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac gaaaattgct
    21001 tcttaaattt ttgtgaaatt tcggcttatt tcttggagat aaaagcttct gtcccagcct
    21061 cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt ataggaaaaa
    21121 atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac aaaaaagaca
    21181 ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc ctttgacaaa
    21241 actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat caagatacca
    21301 aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca aaaagcttca
    21361 gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa gagcaaaaag
    21421 atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat acgatgatgg
    21481 ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa tgtatttcgg
    21541 atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa tacaataact
    21601 ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt tgtcacgatg
    21661 attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa ataaccatcg
    21721 ttttatatag ggaattttct gaaaattgtt gcatattttt cgaaattttg ttacttttaa
    21781 aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact atctttttat
    21841 aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc ccgttcattt
    21901 gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg ttaccggtat
    21961 cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag gtgtatatga
    22021 attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc tttacttagt
    22081 tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga ttggtttagc
    22141 accatttatc gatcctagca gacagacgct atatctagca attttcagta ttgtattttt
    22201 tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg ttggaaagtt
    22261 cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt ttttgaatcc
    22321 actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg cttttttcac
    22381 agggtttact caaggatata acacattaga tgcgcttgct gctttagcat ttgggatcat
    22441 cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg ctaaagacac
    22501 gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt tgctcgctta
    22561 tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga tcacgctggc
    22621 tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat ttatcgttat
    22681 attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa cattcacaga
    22741 tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc ttccttgttt
    22801 agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt tgatgttcat
    22861 ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt ttaagcagcg
    22921 tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt ttgatgggtt
    22981 gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt tatatgttgc
    23041 ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag cagttatcgg
    23101 ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag attgataaaa
    23161 gaggttgtga aagagacgtt cagccttgag caataaaaag taattttgaa aaatggcttc
    23221 tcacattttt gcataaattt gacttaatac cgagaaactg acttttgaac accgtttatt
    23281 gagaggtcgt gaaaaagccg cttagtttca agtattaaga aatattttgt gaaaatggct
    23341 tctcacattt ttgcataaat ttgacttaat accgagaaac tggcttttga acaccgttta
    23401 ttgagcgtct ataaactgac ctgagtatta gagaaacaaa acggcaaagt agctccatat
    23461 actttgccgt tttgttttga actaataccg aagagaagct gatcaaacaa catttctttt
    23521 ataatataga ttttagacat atatatgata taaaaatagt tttcgcaaaa
//
LOCUS       DAFELO010000042        23012 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFELO010000042
VERSION     DAFELO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.042"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(260..1492)
                     /locus_tag="LP452_001776"
     CDS             complement(260..1492)
                     /locus_tag="LP452_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL2014502.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1747..2316
                     /locus_tag="LP452_001777"
     CDS             1747..2316
                     /locus_tag="LP452_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2014503.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2494..2934)
                     /locus_tag="LP452_001778"
     CDS             complement(2494..2934)
                     /locus_tag="LP452_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL2014504.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3092..3856
                     /gene="map"
                     /locus_tag="LP452_001779"
     CDS             3092..3856
                     /gene="map"
                     /locus_tag="LP452_001779"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL2014505.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3888..4811
                     /locus_tag="LP452_001780"
     CDS             3888..4811
                     /locus_tag="LP452_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL2014506.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4887..6026
                     /locus_tag="LP452_001781"
     CDS             4887..6026
                     /locus_tag="LP452_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL2014507.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6019..6819
                     /locus_tag="LP452_001782"
     CDS             6019..6819
                     /locus_tag="LP452_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2014508.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6819..7646
                     /locus_tag="LP452_001783"
     CDS             6819..7646
                     /locus_tag="LP452_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2014509.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7624..8358
                     /locus_tag="LP452_001784"
     CDS             7624..8358
                     /locus_tag="LP452_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2014510.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8458..9324
                     /gene="rfbA"
                     /locus_tag="LP452_001785"
     CDS             8458..9324
                     /gene="rfbA"
                     /locus_tag="LP452_001785"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL2014511.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9338..9910
                     /gene="rfbC"
                     /locus_tag="LP452_001786"
     CDS             9338..9910
                     /gene="rfbC"
                     /locus_tag="LP452_001786"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL2014512.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9932..10960
                     /gene="rfbB"
                     /locus_tag="LP452_001787"
     CDS             9932..10960
                     /gene="rfbB"
                     /locus_tag="LP452_001787"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL2014513.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11058..11909
                     /gene="rfbD"
                     /locus_tag="LP452_001788"
     CDS             11058..11909
                     /gene="rfbD"
                     /locus_tag="LP452_001788"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL2014514.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11943..13976
                     /locus_tag="LP452_001789"
     CDS             11943..13976
                     /locus_tag="LP452_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014515.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14020..15299
                     /locus_tag="LP452_001790"
                     /pseudo
     CDS             14020..15299
                     /locus_tag="LP452_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15509..16315
                     /locus_tag="LP452_001791"
     CDS             15509..16315
                     /locus_tag="LP452_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014516.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16327..17547
                     /locus_tag="LP452_001792"
     CDS             16327..17547
                     /locus_tag="LP452_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014517.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17537..19123
                     /locus_tag="LP452_001793"
     CDS             17537..19123
                     /locus_tag="LP452_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2014518.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19162..21300
                     /locus_tag="LP452_001794"
     CDS             19162..21300
                     /locus_tag="LP452_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2014519.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21668..22651)
                     /locus_tag="LP452_001795"
     CDS             complement(21668..22651)
                     /locus_tag="LP452_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL2014520.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7216 a         3934 c         4748 g         7114 t
ORIGIN      
        1 cgagtctctt ctttctgagg agagacatca agagtcgctg cgctactgct cctgacctct
       61 ctcctcagaa agaaaggcat ggcagttagg ttcacaacgc aaaacattgt gcttttgatg
      121 aggttgaact tttacacaag attgtggact tgactaaaaa aacggtgggg ttgtttagcg
      181 aaataaagga tctaagccaa tagcttagat cctttattct atgctttata ttattttttg
      241 catgtgtgcg aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc
      301 catcttcacg gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg
      361 tttgacttct attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt
      421 tcacactgaa ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata
      481 atgtattgaa aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga
      541 cttctcctaa acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc
      601 cttgttcggc agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga
      661 tgatggtgcc agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac
      721 tgtcaggtgc tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag
      781 cattatagct tccagcgcct tcccagagat ggtttttagg caatccgata atgatatctg
      841 taccgggagc agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt
      901 ttgctaattt ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag
      961 tcgttttgaa aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga
     1021 agacttttgc tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg
     1081 cttgagttgc cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta
     1141 ctcgatggct atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc
     1201 ttgctccttt ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa
     1261 tacgatccgt tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa
     1321 cgataacctc agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg
     1381 cctgttccac gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt
     1441 ctgcaattaa gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa
     1501 ttcctcctca ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa
     1561 caatgaggct ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat
     1621 gttactactt gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa
     1681 aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt aaggagatta
     1741 aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc tgcttatgaa
     1801 gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa caaaatgaat
     1861 tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag acaagctctt
     1921 tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca tacagggaat
     1981 acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa gaagttgtat
     2041 cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt ctcttatact
     2101 tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga agagcagatc
     2161 gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt gatgtcttca
     2221 agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag tatagatgct
     2281 atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt tccacaaatc
     2341 agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac agcgtatgca
     2401 tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca tacagattga
     2461 ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta gttctttagc
     2521 gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt taacattttc
     2581 ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca ccgatttgca
     2641 gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc ccaatagatc
     2701 caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc catcatcgta
     2761 tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat ctacttgggt
     2821 acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa caatatcggc
     2881 gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag ccatgatgtg
     2941 cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat tgaaaagaaa
     3001 ataaaaccta gagacacctg gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa
     3061 tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag cacgtgaaat
     3121 tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc tgcgtgattt
     3181 tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact atatcgagag
     3241 tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga cttgtatcag
     3301 tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa acggtgattt
     3361 ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt gttgggccta
     3421 tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga agaaagcttt
     3481 gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg gtcatgctat
     3541 ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg gtcatggcgt
     3601 tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg gaaaagggtt
     3661 gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg gaacttggaa
     3721 aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac tttcttgcca
     3781 atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat cacaaggaga
     3841 agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg aaagtttgga
     3901 acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa aagcgcatca
     3961 ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc ttgtctttat
     4021 ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat caagagactg
     4081 tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc ggtcctgcaa
     4141 tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca ttagcggctt
     4201 tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg
     4261 tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc attgtcttgt
     4321 tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg attttggatg
     4381 ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa actttgaagt
     4441 ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta attgtcccta
     4501 atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact gtcggctgga
     4561 tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg atcagcggtt
     4621 atcagatcat aggaagtttt attgtactca tgctttggct taattttgct gctacgatca
     4681 tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt catcaaatca
     4741 aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa ttttctcata
     4801 aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta taataattat
     4861 gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt ataagatttt
     4921 ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt tcagtacaaa
     4981 caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg ccttcagcgg
     5041 gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc cgtaaaatta
     5101 tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata gtcacaggag
     5161 tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc ttgattgctt
     5221 ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt ccttttattg
     5281 gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc ttcggtatca
     5341 cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt tcattgatcg
     5401 gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca gtgtatattc
     5461 cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac aatttttatc
     5521 cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg atggccgtcc
     5581 tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg ctgatcgtca
     5641 tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca aaccgtgttt
     5701 ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta ggctttactc
     5761 ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc acagctatgg
     5821 tcatgaatta tactggaaga atcggaacga tcatattgat tattgccatg ttgtttgctg
     5881 caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt attttaaaaa
     5941 cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga
     6001 aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat tagtattgtg
     6061 acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac tgtaaaagat
     6121 gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta caaaaaaaga
     6181 ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga aaattccgga
     6241 tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg cattatttgt
     6301 aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt attgaaagaa
     6361 catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac aactcagcac
     6421 ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg
     6481 gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt gcctactgat
     6541 gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat tgataaattt
     6601 aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt
     6661 ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc agttatccat
     6721 ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt gacttctatg
     6781 atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa atattagtag
     6841 tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca ttaagcaaag
     6901 cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa aggcagcaaa
     6961 atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat cctggtttag
     7021 cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc
     7081 tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa gtaaccttta
     7141 cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa gtttctcccc
     7201 tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa ggcccaacag
     7261 ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga
     7321 ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg ttttggaaag
     7381 tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat gatctttcgg
     7441 ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa gcagaaaatc
     7501 gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa ttattgttaa
     7561 gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg actttgcgct
     7621 catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat
     7681 ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc agcagaagat
     7741 gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt aactgaatac
     7801 gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt gatcgctaat
     7861 tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga ccaagatgat
     7921 gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa tccggatcag
     7981 ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat ccatgattcg
     8041 tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag aagtcactat
     8101 attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc gatcccgtca
     8161 gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac tgggttccta
     8221 agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat tgaaacacat
     8281 tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt gatcaaacga
     8341 aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg acaaatataa
     8401 caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca gatatccatg
     8461 aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac aaaggcaaca
     8521 tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat gtctactctg
     8581 atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac accacgtttc
     8641 aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc agtacaagaa
     8701 agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg agatgatagt
     8761 gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa aatgttgcaa
     8821 cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa tgatccagaa
     8881 cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga agaaaagcct
     8941 gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa tgaggtaatt
     9001 tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac ggatgtcaat
     9061 aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg atttgcttgg
     9121 cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga aacaattgaa
     9181 aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat gggctacatc
     9241 gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata cggtcaatat
     9301 ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag atacaaaatt
     9361 aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt tcttcacaga
     9421 aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg ttcaagacaa
     9481 ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa aaggagaagc
     9541 tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag tagtggatat
     9601 ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg aacataatca
     9661 tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga caccaaatgt
     9721 gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg gcatcgcatt
     9781 tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga ttatgtcgga
     9841 aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat ttgtttacgg
     9901 agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta ccggaggtgc
     9961 cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag aagtgcatgt
    10021 cactgttttg gataaactga cttatgcagg aaacaaagag aacttggctg gacttcctag
    10081 cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg accgtctagt
    10141 aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata attcattaaa
    10201 agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt tagaagcttg
    10261 ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg gcgatcttcc
    10321 attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca ctgcggaaac
    10381 accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc tgttagtaaa
    10441 agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa acaactatgg
    10501 tccttatcag catattgaaa aatttattcc tcgccaagta acgaatatct taagcggtat
    10561 tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata cgaatgatca
    10621 ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg
    10681 tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa tgatgggtca
    10741 accaaaagat gcgtacgacc atgtaaacga ccgtgcagga cacgacttgc gttatgcaat
    10801 cgattcgaca aaattacggg aagagttagg atggaagcct gagtttacga acttccgtga
    10861 tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga aaaaagaaaa
    10921 agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagagaaa atgtcactgt
    10981 attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc
    11041 aaggagcgga taatcatatg tttttattaa ctggaggaaa tgggcaacta ggtacagaat
    11101 tgcgccattt attagatgaa aaaaatctgc aatatgtttc aacagatgca aaagacttgg
    11161 atatcacaga tgccgagaaa acaatggctt acatcacaga attaaaacct gatgttattt
    11221 tccattgtgc agcttacaca gcagtagata aagcagaaga agaagcaaaa gaattagatg
    11281 agaagatcaa tgttgatggt acaagaaatg tagcacttgc agcaaaagct gtgaatgctc
    11341 aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat gtagaatacg
    11401 aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa
    11461 aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg gttttcggga
    11521 tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc gacaaactga
    11581 ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca gaatttatgg
    11641 tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac gaaaatagct
    11701 gttcttggta tgaattcgca aaagaaatat tgaaagatac agatgtagaa gttgcacctg
    11761 ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc atgagcctga
    11821 aaaagacaga agagttagga tttgtcatac caacttggca agaagcactt gcgcaaatgc
    11881 tggaagctgc caaaaaaatg aataagtaat ttccttttat caaatagaat ttggagcatg
    11941 atatggaaaa acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aaccaggcag
    12001 attcgaattt ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca
    12061 tcgtggtcat cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg
    12121 tgtcgcaaag agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc
    12181 ggtcagacga atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttc
    12241 cgttggtgaa cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc
    12301 ctgtaaagga tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa
    12361 gagatcgagg actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat
    12421 tcgaagtact gatgatatta acgaagagaa aaaaagctct atcgctaatc ggtgcatttg
    12481 cgctgacatt ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt
    12541 tctttacttt gggactgatg actgcttttt atcattattt ctacaatcat gataaaaaat
    12601 ggctacgaac gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga
    12661 tttacccagc ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc
    12721 ttatttacta tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg
    12781 cagttttatt tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt
    12841 tagcttcttt gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga
    12901 ttggcgaatt tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt
    12961 cgaataactc tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc
    13021 catttgtgtt atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga
    13081 ttttttgtct gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa
    13141 tcactctgct gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt
    13201 gtttattatc gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt
    13261 ataaattcat tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga
    13321 aaatggataa ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga
    13381 ttttagcttt tgcattattt ggctggagac gcttgtttta cctcgctttt gccgccttag
    13441 ttatcgtatc tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa
    13501 aaacactggc aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt
    13561 ctgaagatga attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac
    13621 gcttttatcc agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt
    13681 attataatcg gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt
    13741 tagatcgtcc agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga
    13801 tcgactatgt gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt
    13861 tcacgcagct atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg
    13921 attatccaga tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg
    13981 ctagctgaga ataatcctga aatgttagta gaaaggagag tggagataat tcaagcaatc
    14041 gtacaaaaat ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttact gacgctagtc
    14101 ttttttgttt ggacagcagg gttaggtttt ccagcaactt tgtttatata taacaatagt
    14161 tcattactta tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa
    14221 ggcgtcgatt ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt
    14281 cgggaattaa gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt
    14341 tgtttagtaa ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc
    14401 ttttcggtct tcctcggaat cacgtttatc gtgtatttac agagatccaa gtaccagaag
    14461 gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat acgaatacaa
    14521 tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt tcttttgaac
    14581 gatggtatgt acgaatcatc ggtgcagtag cagttgttgc agcttttctc gggatttggg
    14641 tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg gatcttttac
    14701 ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ttacacggtt acgattcttt
    14761 tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg tttaccggtc
    14821 gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa caaattttac
    14881 taggaatgaa aacatttatc ttccaacgag gaaaccaatt tttaggaaac cacaatagtt
    14941 ataattctat tttgaatatc tatggattga taggatttgg gattgctgct ttgctgctta
    15001 tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag atgactttta
    15061 tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg acttcataac
    15121 catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat gatgaaccag
    15181 aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact tatgatgata
    15241 catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc tatcattgat
    15301 aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat
    15361 tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat agaaaattaa
    15421 gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact tgactgtaat
    15481 tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat ttatgaagga
    15541 catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca aatcaagata
    15601 tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta ctgtattgat
    15661 ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa aatacccatt
    15721 tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg catttgcatc
    15781 aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat tcaatattcg
    15841 tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct tcattctgat
    15901 tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt tacagctgat
    15961 ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga cggcatcgat
    16021 ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg
    16081 gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa gcatattgaa
    16141 attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg
    16201 gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag taatgttcgt
    16261 aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc tgtaaggaga
    16321 atagctatga cagaatatgc aattagacta aaagatatca caaaatctta taatatgtac
    16381 gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat
    16441 gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc
    16501 gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg agtactgaca
    16561 ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga actaggatca
    16621 ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat ggttctagga
    16681 ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc tgacatcgga
    16741 gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg tctagctttt
    16801 gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt ggcagtaggc
    16861 gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa ctcgggaaag
    16921 acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga tcgttcctat
    16981 tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac gacgaattat
    17041 gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa ccaagtagaa
    17101 cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga taaagacaaa
    17161 aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac ttatgaagta
    17221 aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt cgacaatttt
    17281 tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa aaaaggagag
    17341 aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata ttattttgac
    17401 gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa gtacatgaac
    17461 ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca taaatgggaa
    17521 gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac aaagcagtgt
    17581 gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg gtcccggaaa
    17641 cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca ttgttgagtt
    17701 agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt atggtgatat
    17761 cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg tcttgtttgc
    17821 ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca gagaattttt
    17881 gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt ctgtcatgat
    17941 cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg aaacccataa
    18001 ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat tattcattaa
    18061 tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga
    18121 attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag tctaccaata
    18181 ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc cacaaaattc
    18241 taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa caatccaaca
    18301 aataccattc aataatttca caggggaaaa tgagacacta agtttccccg tatcagaaac
    18361 aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat tcagcgcaga
    18421 agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa cagtaaatga
    18481 ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata tttctgggaa
    18541 agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa caatgaaaga
    18601 attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg agaaaaatga
    18661 cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag accatacgtt
    18721 aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga caataaaaca
    18781 tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag aactttctat
    18841 atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca tcggatgggg
    18901 aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc aatcgccatt
    18961 tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc tcgcaaaagg
    19021 aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat tcttagtgga
    19081 agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga ttaacttata
    19141 aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc
    19201 actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga tgaagtgacc
    19261 aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc
    19321 gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag cggttttaag
    19381 gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac agcgagacat
    19441 caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg cattgaatct
    19501 tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag gactcatggc
    19561 gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat cgactatgca
    19621 gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga agcacaagga
    19681 tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga aatcaaatgg
    19741 ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga actatgtatc
    19801 agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta tcaagcaaaa
    19861 gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct ggcaactaat
    19921 tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga tgatgaatta
    19981 ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga actggatgtg
    20041 atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc gcatttcaaa
    20101 gcagactggt cgcctgatac attgatggga aataattata tctctcactt aggcgtatat
    20161 cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg ttctcaagat
    20221 tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta ccacattgac
    20281 cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg cgaagccaaa
    20341 agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag aagaaacatc
    20401 aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta tgacgtcttg
    20461 caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga tctaaaaact
    20521 tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat tattgcggat
    20581 aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca tcagctgaaa
    20641 gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat taacaattta
    20701 gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac agaagtgatt
    20761 gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat cggctgtgta
    20821 ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt attaggaatc
    20881 ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg ttatttcggc
    20941 cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat ggtcaaggca
    21001 gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt caacgatgtt
    21061 gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca tcaagcagaa
    21121 ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa acaaagacgt
    21181 tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc gcatgatcca
    21241 ttttacaatg ataatttaac aaaagaagga atcggcgact tttcgttgag accagattaa
    21301 actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt ttatagtttt
    21361 ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg ggacagaagt
    21421 gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    21481 tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt taaagaatga
    21541 gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc gccaatagat
    21601 acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata ttttatttta
    21661 atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc actctatcaa
    21721 ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta gcgtcaatat
    21781 aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa tctaataccc
    21841 accataaata gccatatctc atattttgta agtcttctaa agcactggtt ctaggtttga
    21901 acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc cctttattta
    21961 ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca tatcctgccg
    22021 tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta tccattgtaa
    22081 actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt atccctatag
    22141 gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc tgatacaaca
    22201 agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt ataccttttc
    22261 ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag acttttgtcc
    22321 atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg attgtaatag
    22381 cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct aatactttat
    22441 catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa gatacaatac
    22501 ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc cattcatctg
    22561 tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt tgtcgaaaac
    22621 caataaaatc atgcaactct tgtttattca taagatatta cctcactttc ttctataagc
    22681 ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac aggtattctt
    22741 atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg ttcactttat
    22801 gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca ttagtgaaaa
    22861 tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt gtataaagct
    22921 ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt tttaaaggtg
    22981 actatggtaa gataatagag atattatttt gt
//
LOCUS       DAFELO010000043        22129 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFELO010000043
VERSION     DAFELO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.043"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..523
                     /locus_tag="LP452_001796"
     CDS             <1..523
                     /locus_tag="LP452_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL2014521.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="LP452_001797"
     CDS             523..2109
                     /locus_tag="LP452_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014522.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="LP452_001798"
     CDS             2128..2349
                     /locus_tag="LP452_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL2014523.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="LP452_001799"
     CDS             2346..3107
                     /locus_tag="LP452_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL2014524.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="LP452_001800"
     CDS             3487..3996
                     /locus_tag="LP452_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL2014525.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="LP452_001801"
     CDS             complement(4071..4610)
                     /locus_tag="LP452_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL2014526.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LP452_001802"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LP452_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL2014527.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5623..6474
                     /locus_tag="LP452_001803"
     CDS             5623..6474
                     /locus_tag="LP452_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL2014528.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            6502..7137
                     /locus_tag="LP452_001804"
     CDS             6502..7137
                     /locus_tag="LP452_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL2014529.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="LP452_001805"
     CDS             7157..7930
                     /locus_tag="LP452_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2014530.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="LP452_001806"
     CDS             8338..9135
                     /locus_tag="LP452_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL2014531.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="LP452_001807"
     CDS             9113..9526
                     /locus_tag="LP452_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2014532.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="LP452_001808"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="LP452_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL2014533.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="LP452_001809"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="LP452_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL2014534.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /gene="maf"
                     /locus_tag="LP452_001810"
     CDS             14303..14863
                     /gene="maf"
                     /locus_tag="LP452_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL2014535.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LP452_001811"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LP452_001811"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL2014536.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="LP452_001812"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="LP452_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL2014537.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVLHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="LP452_001813"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="LP452_001813"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL2014538.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18565..19167
                     /gene="ruvA"
                     /locus_tag="LP452_001814"
     CDS             18565..19167
                     /gene="ruvA"
                     /locus_tag="LP452_001814"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL2014539.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19180..20181
                     /gene="ruvB"
                     /locus_tag="LP452_001815"
     CDS             19180..20181
                     /gene="ruvB"
                     /locus_tag="LP452_001815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL2014540.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20210..20788
                     /locus_tag="LP452_001816"
     CDS             20210..20788
                     /locus_tag="LP452_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2014541.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20840..21322
                     /locus_tag="LP452_001817"
     CDS             20840..21322
                     /locus_tag="LP452_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL2014542.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21439..21813
                     /locus_tag="LP452_001818"
     CDS             21439..21813
                     /locus_tag="LP452_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014543.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7238 a         3991 c         4788 g         6112 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt taattggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 tttgcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt
    18541 gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt tacctttatc
    18601 aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc actaggaaat
    18661 ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca tcaggtaata
    18721 cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca actgtttttg
    18781 cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa
    18841 gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt gacgaaattt
    18901 ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg aaaattcggt
    18961 gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa agacgctaca
    19021 gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg agaaatcaaa
    19081 cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta tctacgccaa
    19141 gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga atacttgtta
    19201 tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc acagctgctt
    19261 tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat cgaagctgcc
    19321 agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa
    19381 acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac gacgagtggc
    19441 cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga agcaggggat
    19501 gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat gctttattca
    19561 gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc acatccggtt
    19621 catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg tatgctttct
    19681 gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga cgaacaggat
    19741 ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat tgaagaaggg
    19801 gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg cttgctcaaa
    19861 cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc tatagcggat
    19921 caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga ccagaaattg
    19981 ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac gctttcagta
    20041 aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct gatccaaaaa
    20101 ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta tgaacatttt
    20161 ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa tgagtgatac
    20221 aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt
    20281 tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta
    20341 ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg atgcattgca
    20401 ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat tgaaaatgct
    20461 gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg acccagaaat
    20521 tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt ttgaacaaat
    20581 ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt ttttttctta
    20641 cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa caccgatgga
    20701 aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt atcgtcttta
    20761 tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa agcaataatc
    20821 gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa atatttgccg
    20881 ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa gggaagcaga
    20941 attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg
    21001 aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg ctcggcaaat
    21061 cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag aaaatgtaga
    21121 agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt ttttaagtag
    21181 cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata actttgatga
    21241 gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa
    21301 taaaataaca aacaatttgt aaagttaacg ctttcttttt aaggcgatgt tagagcattt
    21361 ccttgttttt ttgtaaaatg ttaggtaaaa taaaattaat taagtaacac tttcttagct
    21421 aaaggaggag tgcattcgat gaacgaagag acagtatcgc caatgaagaa gcttacgaag
    21481 ttagttactc atacttcgta tcaacttttt gattcttttg taaatgacac tgaagaacat
    21541 gctaagaatg tacgattgca aggaaagttt attcttgagc tgcaaaaagc tgctactgaa
    21601 catagtctag ttatcttaca agtaagagca aatcgtttga gtagcaagta tgaaacgatc
    21661 tcaggctggg tggcgacaaa aacggtcgaa aaagaccgag tgatgatccg cctacaaaac
    21721 gaagagcagc agatccggat ggttcctgta gaacaaattg aaaaaataac aatcctttca
    21781 ccagcagggg aacaggaacg gttatcaaaa taatttatac cagatagaaa agcacgtatg
    21841 agcttgtacc atcaatcatt actaatcaaa aataaccgaa tgctttaagc tgatatcagt
    21901 aaaacggaac gttttaggat gagtatcata gcatgacagc attcggttga ttagttgaat
    21961 cgattggatg gtacaagttt ttttgataaa aaaaggtcct gctagccggg gagctagcag
    22021 gataaggagt taaaaatgaa aaagtgttgt tagggttgtt tgttggtatg cttatataat
    22081 agtcattagt tgtgaatatt ttgtgacgaa agttttcgaa agttgttaa
//
LOCUS       DAFELO010000044        21863 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFELO010000044
VERSION     DAFELO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.044"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(46..705)
                     /locus_tag="LP452_001819"
     CDS             complement(46..705)
                     /locus_tag="LP452_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2014544.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            1021..1965
                     /gene="mvk"
                     /locus_tag="LP452_001820"
     CDS             1021..1965
                     /gene="mvk"
                     /locus_tag="LP452_001820"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL2014545.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1958..2935
                     /gene="mvaD"
                     /locus_tag="LP452_001821"
     CDS             1958..2935
                     /gene="mvaD"
                     /locus_tag="LP452_001821"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL2014546.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2948..4033
                     /locus_tag="LP452_001822"
     CDS             2948..4033
                     /locus_tag="LP452_001822"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL2014547.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            4030..5094
                     /locus_tag="LP452_001823"
     CDS             4030..5094
                     /locus_tag="LP452_001823"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL2014548.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(5343..6146)
                     /locus_tag="LP452_001824"
     CDS             complement(5343..6146)
                     /locus_tag="LP452_001824"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL2014549.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(6166..6915)
                     /gene="kduI"
                     /locus_tag="LP452_001825"
     CDS             complement(6166..6915)
                     /gene="kduI"
                     /locus_tag="LP452_001825"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBL2014550.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVCESCQLQMGYTILEPGSAW
                     NTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPSWSIHSG
                     VGTSNYSFIWAMCGENITYTDMDMVQMDQLK"
     gene            complement(6931..7938)
                     /locus_tag="LP452_001826"
     CDS             complement(6931..7938)
                     /locus_tag="LP452_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBL2014551.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7945..8589)
                     /locus_tag="LP452_001827"
     CDS             complement(7945..8589)
                     /locus_tag="LP452_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL2014552.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(8689..9447)
                     /locus_tag="LP452_001828"
     CDS             complement(8689..9447)
                     /locus_tag="LP452_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBL2014553.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(9574..10725)
                     /gene="fucO"
                     /locus_tag="LP452_001829"
     CDS             complement(9574..10725)
                     /gene="fucO"
                     /locus_tag="LP452_001829"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBL2014554.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10807..11118)
                     /gene="rhaM"
                     /locus_tag="LP452_001830"
     CDS             complement(10807..11118)
                     /gene="rhaM"
                     /locus_tag="LP452_001830"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBL2014555.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(11120..11965)
                     /gene="rhaD"
                     /locus_tag="LP452_001831"
     CDS             complement(11120..11965)
                     /gene="rhaD"
                     /locus_tag="LP452_001831"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBL2014556.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11979..13235)
                     /gene="rhaA"
                     /locus_tag="LP452_001832"
     CDS             complement(11979..13235)
                     /gene="rhaA"
                     /locus_tag="LP452_001832"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBL2014557.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(13232..14692)
                     /gene="rhaB"
                     /locus_tag="LP452_001833"
     CDS             complement(13232..14692)
                     /gene="rhaB"
                     /locus_tag="LP452_001833"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBL2014558.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14845..15714
                     /locus_tag="LP452_001834"
     CDS             14845..15714
                     /locus_tag="LP452_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBL2014559.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15840..16739)
                     /locus_tag="LP452_001835"
     CDS             complement(15840..16739)
                     /locus_tag="LP452_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014560.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16904..17728)
                     /locus_tag="LP452_001836"
     CDS             complement(16904..17728)
                     /locus_tag="LP452_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2014561.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(17739..19034)
                     /locus_tag="LP452_001837"
     CDS             complement(17739..19034)
                     /locus_tag="LP452_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBL2014562.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(19050..19529)
                     /locus_tag="LP452_001838"
     CDS             complement(19050..19529)
                     /locus_tag="LP452_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL2014563.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(19535..20563)
                     /locus_tag="LP452_001839"
     CDS             complement(19535..20563)
                     /locus_tag="LP452_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBL2014564.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(20580..21686)
                     /locus_tag="LP452_001840"
     CDS             complement(20580..21686)
                     /locus_tag="LP452_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL2014565.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6271 a         4850 c         3924 g         6818 t
ORIGIN      
        1 aagtcgctca aataaaggtt taccctttat ttgagcaact gtaaatcaaa tggctacttt
       61 ggtttggcga atctctgcta atttttcttc gatcattttc aagacttctt cttttgcttg
      121 aggatcttcc acaaaattat agcggtctcc atcgatttgg atttttggac tttctttgta
      181 ttcttcatac cattgatcgt aacgtctgtt caattctttg taataatcgt atagacttgg
      241 atcaaaggac aattgttcat acgaacgacc acgtttttcg atacgctcca acatcgtatc
      301 aaaagataca cgaatatgaa ctaataaatc cggatgtttt ttatgggcag catagggaag
      361 ttcttccatc atgttttcca gcagttcatc atacacacga acttctgttt ctgttgctct
      421 tcctagatct gcatttagat ggaaaagcag tgaatcttca taaatcgaac ggtccaatac
      481 attattgtca tctttcattg cttgtttgat gcttgcaaaa cgtttattca aaaaataaat
      541 ttgcagcaaa aaagcatact gttcagggtt tgcataaaat aagggcaaga cttcattatc
      601 atcaatcgat tcataaaatg cctgactatc aaagtgttca gccatcattt ctgttaaact
      661 tgattttccg gcaccaattg tacctgctaa aacgatcacg ctcatttacc tccagctcac
      721 ttcatcacta aatattgtgt ttaactccaa cctttttaaa tataccccca atatctagta
      781 gtgtcaattg tatttttttc tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa
      841 aagtgttttt tctttagaaa acgtattctt agttttactt ctgagatggt tttagtaaaa
      901 taaaggagga atgttttttg agacccttag tggacaaaaa aattcgtgaa gcagcgcaac
      961 tcgttttggg cgctttattt tttgcttaaa aatcactttt acgtgagaag gagttagatt
     1021 atggcaaact atggccaagg agaatcaagc ggaaagatca tattgatggg cgagcacgcg
     1081 gttgtttatg gagaaccagc gattgccttt cctttctatg caacaaaagt caccgcattc
     1141 cttgaagagc tggatgcaat ggacgatcaa ctggtttctt cctactattc aggaaattta
     1201 gccgaagctc ctcatgcatt aaaaaatatc aaaaaattat tcattcactt aaaaaaacag
     1261 catgacatcc aaaaaaactt gcaactgacc attgaaagca cgattcctgc tgaacgtgga
     1321 atgggatcaa gcgctgcagt cgccacagca gtcactcgtg ctttttatga ttacttagca
     1381 tttcctttgt ctcgtgaaat actattagaa aatgtccagc tttcggaaaa aatcgcccac
     1441 ggtaatccta gtggaatcga tgcagccgct actagcagct tgcagccgat ttattttaca
     1501 aaagggcatc ctttcgacta cttttctttg aacatcgatg cttttttgat tgtcgctgat
     1561 acaggaatca aaggacaaac aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc
     1621 cagcctcatg aaactggaca aatgatccaa aaattaggat acttgacgaa gcaagcaaaa
     1681 caagcaatca ttgaaaattc accagaaacg ttggcacaga caatggatga atcacaatca
     1741 cttctggaaa agctgacaat cagcaatgat tttcttaatc tattgatcca aacagcaaaa
     1801 gataccgggg cattgggtgc caaattaact ggcggtggac gcggtggctg tatgattgca
     1861 ttagcacaaa caaaaacaaa agcccaagaa atcagtcaag cacttgaaga tgcgggtgct
     1921 gctgaaactt ggatacaagg attaggagta catacctatg tttaaaggca aagcacgcgc
     1981 atataccaac attgctctaa tcaaatattg gggtaagaaa aatgaagaac tcatccttcc
     2041 aatgaacaac agcctttcgt taacattgga tgcattttat acagagacag aagtcatctt
     2101 ttctgatagc tatatggtag atgaatttta tctagatggc accttgcaag acgaaaaagc
     2161 aacaaaaaaa gtcagtcagt ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa
     2221 agcttcagta atcagtcaga atttcgttcc taccgcagct ggattagcct cctctgccag
     2281 cgggctagct gctttagcag gcgcttgcaa tactgctctt aagcttggat tagatgatct
     2341 ctctctttca agatttgctc gacgcgggtc tggttcagct tgccgaagta ttttcggtgg
     2401 tttcgtcgaa tgggaaaaag gccatgacga cttaagttct tacgctaagc cagtcccttc
     2461 cgattctttc gaagacgatt tagcaatggt tttcgttttg atcaacgacc agaaaaaaga
     2521 agtgtccagc agaaatggga tgcgtcggac agtcgaaaca tccagttttt atcaaggctg
     2581 gttagattcc gttgaagggg atctatatca attgaaacaa gcaatcaaaa caaaagattt
     2641 ccaacttctc ggagaaacga tggaaagaaa cggactaaaa atgcatggaa cgactttagc
     2701 tgcccagcct ccattcactt actggtctcc tgacagctta aaagcgatgg atgcggtgcg
     2761 acaattgaga aagcaaggca ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa
     2821 agttttagtg gaaaacagcc atttatctga ggtacaagaa acatttacca aattatttag
     2881 caaagaacaa gtaattactg cacatgctgg tccaggaatt gcgattattg aataaaggga
     2941 gatttttatg attgaagtat ctgcaccagg caaactctat attgccggag aatatgcagt
     3001 tgttgaaaca ggccatccag cagttatcgc tgcagtcgat caattcgtga cagtcactgt
     3061 agaatctgca cgaaaagtcg gaagtatcca atctgcacaa tatagtggga tgcctgtacg
     3121 ttggacaaga cgcaacggag aattagtttt agatattcgg gaaaatcctt ttcattatat
     3181 ccttgctgct attcgcttga ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt
     3241 ttatgatctg aaagtaacaa gtgagttaga cagttcaaat ggccggaaat atggattggg
     3301 ttctagcggt gccgtgaccg ttgcaacggt caaagccttg aatgtttttt acgcgttgaa
     3361 tttatctcag ttggagattt tcaagattgc agcactagcc aatttagcag ttcaagataa
     3421 tggttcctgc ggcgacatcg ctgctagctg ttatggtggc tggatcgctt tctcaacctt
     3481 cgaccatcct tggctccaag aacaagaaac tcagcattct atcagtgagt tacttgccct
     3541 ggattggcca ggtctatcca ttgagccatt gattgctcct gaagatttac gtttattgat
     3601 tggttggacg ggtagccctg cctctacttc tgatttggtc gatcaagttc accgttcgag
     3661 agaagataaa atggtggctt atcagctttt cttaaaaaat agtacagaat gtgtcaacga
     3721 aatgatcaaa gggtttaaag aaaataatgt aacgttgatc caacagatga ttcgaaaaaa
     3781 ccgacaatta ctgcatgatt tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt
     3841 gaacaaattg tgtaatttag ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg
     3901 tgggggcgat tgcggaatcg taattgttga ccagaaatct ggcattcttc ctttaatgag
     3961 tgcatgggaa aaagcagaaa tcactccact gccgttacat gtctatagcg atcaaagaaa
     4021 ggaaaaccga tgaatcgaaa agacgaacat gtttcactag ctaaagcctt tcatgataaa
     4081 caaaaaaacg aatttgactt cgtacggatc atccataacc ctttgccgca aatagcagtt
     4141 tctgatgtcg atctaagtac acaggcagtt gggtttacac taagcagccc attttatatc
     4201 aatgcgatga ctggcggaag cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc
     4261 gcaagagaag cagatttgat gattgccact ggttcagtca gcgctgcctt gaaagtccct
     4321 tctttagccg atacttatac gattatgcga caagaatatc ctcacgggaa aatcattgcc
     4381 aacatcggtg cagggacttc tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca
     4441 gatgccttac agattcattt gaatgcacct caagagttgg ttatgcctga gggagaccgt
     4501 gattttacca attggaaagt acttattcaa gaaactcaaa cagccattga cgtccccctc
     4561 atcgtaaaag aagtcggttt tggcatgaca agagaaacgc tcaatgatct agctgcttta
     4621 ggagttcata cagttgatat cagcggtcga agcggaacaa gcttcaccca gatcgaaaac
     4681 gcccgtcgct ccaagcggga actgagttat ttagctgact gggggcaatc gacagtctct
     4741 tcattgcttg aagcaaatga agcagacact tctatggaaa tcctagcctc cggaggtatc
     4801 cgcaatgcct atgacatatt taaagccctt tgtctcggcg caaatgccgt gggtacttca
     4861 ggaaccgtac tgacgcatct gatgaatcac ggcgtagaag aaactattat actgatgaag
     4921 caatggcaag aagagcttcg tctgctttat actatggtcg gcgcaacgaa taccgcggcc
     4981 ctacaccaac aatctctgat cttttccgga ccagtgaagg attggtgcga agcaagaggg
     5041 atcgacttag tgaagtatgg aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca
     5101 cttttgtctc acgctctaaa cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa
     5161 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aattccttct tatttcttgg
     5221 agttaaatgc ttctgtccca gcctcttttt tcgaataaat gatgtttaat cagctgtcct
     5281 tcggtattcg ttcaatccgc tgatttcaca acctctccac taaacagtgt actaaagctc
     5341 ttttaaggtt gttttccgat ataggcaagt atgcctccgt ccacatacaa gacatggccg
     5401 ttaacgaaat cagatgcgct agaagccaag aagacagatg gtccagccaa atcgattggc
     5461 tcgccccagc gtgctgctgg cgtacgtcca acgataaatt gatcgaatgg atgacgttca
     5521 ccgttttctt gtgtttcgcg taatggagca gtttgcggtg tagcaatata acctggtcca
     5581 attccattac attggatatt gtattgaccg tattcagaag cgatgttttt cgtcagcatt
     5641 tttaatccgc cttttgccgc agcataagca ctgaccgttt cgcgtcctaa ttcactcatc
     5701 atggagcaga tattgatgat tttaccgccg cctttttcga tcatatccgg aataactgct
     5761 tttgaaacga tgaatggggc atttaaatcc acgtcgatta cttggcggaa gtctgccgca
     5821 tccatttcga tcatcggtgt ccgtttgatg atccctgcat tgttgactag aatatcaact
     5881 gaacctacat cttctttgat ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca
     5941 tcgcagacat aaccttttgc atcgatgcct gcttctttat aatgcgctat accttcatca
     6001 actgattctt gattcaagtt attaaatacg atttttgctc ctgctgtagc taaagagcgt
     6061 gcaatctcaa atccaatacc atagacagca ccggtgacta atgctacttt tccatctaaa
     6121 cgaaacatat ccatactaaa ttccatcatt ttctctccct tatgcttatt ttaattgatc
     6181 catttgcacc atatccatgt ctgtataggt gatattttct ccgcacattg cccagatgaa
     6241 tgagtaattg cttgtaccta cacctgaatg gatcgaccag cttggcgaaa taatggcttg
     6301 ttcattgtct acaactaaat gttttgtttc atctggtttg cccatcatgt ggaacacacg
     6361 tgtatcttca ttggcataat caaagtagac ataagcttcc atacgacgtt catgtgtatg
     6421 acaaggcatc gtattccaag cgcttcctgg ttctaaaatc gtataaccca tttgtaattg
     6481 acagctttcg catacatttg gatatttatg atgagctgga acagaactaa tatagaattt
     6541 agctgggttt tcagcatctt gagaagaaaa tacaacatgt cgtgtttctt ttccgatata
     6601 gtatccgtct tgttttttca tttcttcttt ttgcccatcg atctcgatga atcctggtcc
     6661 accgatattg atcacaccta gttcacgacg ctctaagaag taatcaacac ctagttcttt
     6721 gtctaatgtg atttctaatt ctttttcagt aggtgtcact ccaccaaaga tcattcgatc
     6781 gttgtgcgta taagtcaaac tgattttccc tggttcaaat accttttcta ctaaaaattc
     6841 ttttctcaat tgttccgttg aataatgacg gatatcttct ggactgtgtg tataacgtgt
     6901 ttccatttct tgcattttct tctactcctt ctatcgaatg atttttccag agccgcatgc
     6961 taaaaattgt gcgacttctt cttgactaaa ttggttgcag tccccatgga tcgtatgctt
     7021 caaggcagaa gctgccgtag caaaatcaat gatttcttgc ggagacatat tggataaaat
     7081 cccatggagc acgccacctg caaaagcatc tccgccacct actcgatcca cgattggggt
     7141 gattctatgg acttgggatt ccacatattc atggttcatc cacaatgtcc ccttcaattc
     7201 attttcactt gctgaatgaa cggtacgctt ggtcgaataa aacacctgaa tattcgggaa
     7261 cttttcctgc attttttgat aataataaat tagaggttgt tcttctgctt ctggcgcttt
     7321 ctcgatacct aacaaataaa gagcatccat ttttccagct gagcagatat ctactaaagg
     7381 taaaagctga tggattactt cgctggcttc ttgctgtgtc cataattttc cacgatagtt
     7441 gatatcgaag ctgatcaggc agccagcttt ttttgcagct tctatcacct gtttcgtcat
     7501 tttttgccat tttttagaca aagctggggt gattcctgaa acatgaaata tatctattcc
     7561 ttggaataac tcctctagcg accattccaa atgatccatt tcagcaaaac ttgatccggc
     7621 acgatcatag atcactgagg cggcgcgttc gccactacca gtttccatat aataagtacc
     7681 tagtcttgat ccaccagata atacctgttt cgtatctact ccataacatt gcaagtgctt
     7741 tttgactgct tttcccaatg cgttatcagg tacttttgta gcaaaaatcg cttcatgtcc
     7801 gtaattagct aaagaaattg ctacgtttgc ttcccctccg ccataatggg cagcgaatcg
     7861 ttcacttgaa tcgatacggg ttccctgctc tgtggacaat cgcaacatga tttctccgag
     7921 tgtcactact tttcccataa gcagctacct cttgatttct ttcatttttg ctgcatactg
     7981 ttgcgcgact tccgttactt tgtcaaaatc tcctgtagct gcgggtgcta aaaggttccc
     8041 acctactccg actgttacga caccagcatc gaaccactct ttcatattgt ccagactcac
     8101 accgccagtt ggcatgatat tgacttgagg catcggcgcc ttgaaggcag aaatgatact
     8161 tggtccataa gcgcttccag gaaatagttt gacgatatct accccgcttt tcagcgctgt
     8221 tttgatctcg gtaatagtca tacatcctgg taagtaagga atttgataca gattgcacat
     8281 ctctgccgtt tcttgatcaa aggaagggct aacaatatac tctgcgccag ctaagatagc
     8341 caagcgagct gtgacagcgt ctaatactgt gcctgcgcca atcactattt ctggttggtc
     8401 tttgtagaat gacaaaagtt ctttgattac ttgatctgct tgagggactg taaaagtcaa
     8461 ctcaatacca gttagaccac ctttgatgat tgcgtggcaa gcattcaaag cttcttcttt
     8521 gctttttccg cgtaccaccg caatcactcc ggcgtttttc aatcgagata aaatatcaat
     8581 ttttttcatc ttctccacct atttcctatt tagaaatttt attttataac ttggaaatct
     8641 atacccatat tatacggcag aaaagaaaaa ttgcaaacgc ttttttcatt atttttgaat
     8701 tctttgctcg atttcgcttt ttgtttgctt gagggcactg attatttgtt cttttgtctc
     8761 atctgttaaa cgatatttcg gcatacttac actaaatgca ccaatgattt gttcgttttt
     8821 cacgattgag gctcctatac aaaaaatatc acgttcgatt tcttcatcgt caaatgcgat
     8881 gtgggtttct tttaccgttt ggatttcctt tttcaaccgt aatgcattgg tgatcgtata
     8941 ttctgtgtaa gggatcaacg gatgcttttc aagataatca gtgtactgct cttcactgaa
     9001 ttctgctaat acggctttgc ccatcgcaga attgtataat ggtcgggtga ttccgatttt
     9061 tgaagacatg cggatcgttt gatgttttgg atctagtttg ttcacgtaca agatctcatt
     9121 gttgtccaat acccctaagt ggatagtctc atcgatcttt tgctgcagct gttctaaaaa
     9181 cggtaaagtc aactctacca aatcgatttg ttcaatgttt ttattcgcgt agcgaataag
     9241 ctttgcacct aaacgatagt ttttctctga atttttattt acgtagccga tcatcaacaa
     9301 agtatctagt attttcaaga ctgtagaaga ggtcattccc acaccctttg cgatcgtctg
     9361 caaagagaca tcttggtttt ccgctaaata gtctagtatt ttagaggctt tgatcaatac
     9421 agttccataa ggctttttat tttccatata atattctcct atttctttat aagaaaaatt
     9481 atagcggaaa taccatcaat ttaaaagaga tatccagata taaactacca tttctcttta
     9541 caggcaattt aatgttgaag atccgcttcg tttttacatc aaactttgat ataaagcaat
     9601 gatttcttct actgtcgttt ctcttggatt tcctggtgtg catacatctc gcaaagcatc
     9661 ttctgcaatt gctggaatat ctgcttcttt tacacctaat tcagaaatgg tttctgggat
     9721 tccaaccgct ttgctcaacc gatcgatctc accacaagcc gcttctctta catcttctaa
     9781 agacatttca gctgctcctt taatccctag aacaagagcg atctcacggt acttttctcc
     9841 agtatattct ttgttatatt tcatgacagt tggcaaaagt gctgcacacg ctacgccatg
     9901 aggaatgttg taccatgcac ttaacgggtg agccatcccg tgtaccagtc ctaaaccgac
     9961 atttgagaag cccattcccg caatatactg tcctaatgcc attttttctc gcccattttg
    10021 atcgccatct acagaagctt ccaaagaatg gccgatgatt tcgattgctt ttaaatgcag
    10081 catatcagtc atttcccaag cgccttttgt gataaaccct tcaatagcat gcgtcatagc
    10141 atccatacct gttgatgcag ccaatttttt cggcattccc atcatcatat cgctgtcgat
    10201 gaacgcgacg attgggatat catgaggatc aacacaaaca aattttcggt gatttacttc
    10261 atctgtgatg acgtagttga tcgttacttc tgctgctgtt ccagaagtcg ttggtatagc
    10321 taatataggt aaacatggat ttttcgtatc agcaacacct tctaaactaa tgacgtctga
    10381 aaactctgga ttcgtcaaaa taatcccaat ggcttttgcc gtatcgattg gcgaacctcc
    10441 accgattgcg atcaaacagt ctgcctctgc ctctttaaca aaagccactc catcttttac
    10501 attttggatc gttggattag gtacgatccc gtcgtataaa gcataagcga ttgctgcttt
    10561 atctagaagt tctgttactt ttgttgctat accgtgctcg atcaatcctt tgtcagtaat
    10621 gacagctgct ttgccgaatc ctcgctgctg aaattcaggg acgatcgact caatcgctcc
    10681 ttttccaaaa tacgatgttt catttaaaat cattcgatta ctcattttct attcctccta
    10741 atttgtatta tttttcacaa taaaaacgaa agaaccccaa gcaaccggtt gcccagttgc
    10801 ttgttgctac agttcaaaga ctggcatcaa ttcccaactg actggagagg catctggatt
    10861 tgtctccatg atatcttcca tgtacttcca ccatttttga ttgatagatg tatttgccat
    10921 tttttcccat aacttttcat cttcgatcac taagtaacca aataacaaac ttgtactcgg
    10981 atcaagatag atcgaatagt cgatgacccc gtgttcacga agtgctgtac gcatttctgg
    11041 ccaaagttca tcatgtcttt ttttgtattc ttcttgtttt tctgggtaaa ctttcatgca
    11101 aaaagctttt ttcaccatgt cagacacctg ctttcgtatg aacttcctcc aaataacctg
    11161 cttttggagt gacaccaaac gcatctgcta aatgccacag gttttcgtcc gtgattgttt
    11221 gcaagattgg tccttgcgct ttgacgtacg tatagacttc tgctgccttt tctgctgtct
    11281 cgatcaaacc aaagacttca tccatgtctt ttcctgaccc gtagattcca tgttgtggcc
    11341 ataaaaccaa tcgagtttct ttcatttttt cagctgttgc ttccccaatc tcatttgttc
    11401 ccggaaccat ccacggaatg atggaaattc cttctggaaa aacaactaag cattccgtac
    11461 acatttgcca tagcgtacga ctaaaggaac gttcgtctaa ttcatgcgta aaactcatcg
    11521 ctaatagatg actagcatgg caatgcataa cgatccgatt atctgggtct acttctaaac
    11581 gggcgatatg actcatgaaa tgacttggca actcgcttgt aggaaggcat tcgttttcca
    11641 gcccccacaa aagttccagt gttttgccat cttcagcaac tcgaacgatt ccaagattct
    11701 cggcaggttc gctcgctaca tttttaaaat atcttcctga accagttaca ataaaatagc
    11761 atcctgctaa acggctggca tcaaaaatca tcgagatgcg gcggatcact ttttcaggat
    11821 ccaagaacgg tgtgatctct tcttctttta gtaaataaga aatattcccg ccatttcttt
    11881 catcccatcc caagcgataa agattcgacg tcgtttcgat catttctttc acataaggcg
    11941 cttgtaaaat atctattttt ttcaatatag ccacactcct attttcttaa ttccaacacg
    12001 tctttttgat attcctggac ttcttccagc caatccaaac caactggaac ttgatttttg
    12061 taacagaaat aattccatac atcggcatac gggaaatctt tcaattcttc cgtataagcc
    12121 attcttgaag taaagtcaaa agccagctcc atttctttca atcgctcaac tggttccagc
    12181 atagctttca gcagtgcttt ttgtgtattt ctcgtaccga tgacccaagc agcgatacgg
    12241 ttgatcgttg catcaaagaa atccagaccg atatttgttt tttccaacaa ttcgttacgg
    12301 actaattctt tagcgatttc ctgcaattca tcatccatca aaaccacatg gtctgaatcc
    12361 caacgcactg gacgggaaac atgcaacatg attcctttgc tgaataaagc taatgaagat
    12421 aatttattgg aaatcacttc tgttggatga aaatgtccag catctagaca aacaagtttg
    12481 tcacgagtca acccgtaacc catataaaat tcatgggaac cgactgtata agcttcagcg
    12541 cctaaaccaa atagtttgct ttctactgca tcttgtacat accgttcatc taactcttct
    12601 gtaaaaattt catcgagtga ttccattaaa cgttcccgtg gggtaaaacg atcaatcgga
    12661 ttatctttca tcccatcagg tacccaaaag ttattgatac ttgtttgccc agtttctttc
    12721 ccaaagtact ccgcgatttt gcggcttctt ttcccgtgtt cgatccagta ttcacgaact
    12781 tctttatctg gatgagccaa tgtaaagccg tctttcatca ttggatgcga aaagaaagtc
    12841 ggattgaagt caagaccgat ttcttctttt tttgcccact ctacccattt ttcaaaatgt
    12901 ttcggctcaa tttcatttag atcgacagat tcttctgtat ccaaatagat agcatgaagg
    12961 ttcaatttgt gtttgcccgg tatcaacgaa taagccttct ccagatcttg tctaagctct
    13021 tcaggtgttc gtgcggctcc tggaaaattc cctgtcgcca tgattccacc ggtcaattcg
    13081 ccatccggtg tcaagaaacc tttgacatca tctccttgcc agcaatgcat agaaattttg
    13141 atgtcctgta tcttttctag tacagcatct gtatctacac cgatagttgc atatttttct
    13201 tttgcttgtt cgtaatttct ttcaatcgtg ttcatacgat tactcctttg attttattca
    13261 cgtaccattc catcgtgttt tgcttatttt catctgggta aaaagtttca catggaaacg
    13321 actgtttgat tacatttctt gcttgttcta tattcgaaaa ctgacctgta ctgagcattt
    13381 gcatggcaac attcccgatt gctgtggctt cactaggtcc tgcttctaca atgattccgg
    13441 cataatctgc tgtcaattga ttcagtagac gattattcga tccgccccct acgatgtgca
    13501 gcttttccaa tttcgttcca gttaagtgtt ctactttgat cagctcttgt gcgtaacaaa
    13561 gagcaagatt atcatacaca caacgagcga tagctcccga tgagacaggg actggctgct
    13621 ctgtttcaaa acagtattgc tgaatctctt ctatcatatt tgggggattc aaaaatcgag
    13681 ggtcattgat ttccatcact tgctgcagcg gtttttcttt tgcagcaagc tctgccaatt
    13741 ccgcataact gtactcgtac tcttgcattc gggcaacttc ctggatcaac cacatcccca
    13801 tgatgttttt caaaaaacgc accgtgttat gggcacccca ttcattcgta taattttcag
    13861 aaaaagcatt gcgagaaaca ttggcgatct ttgtttcaat ccctaaaagc gaccatgtcc
    13921 cactgctgat ataaccccaa cgttctcctt gacctggcgt tcctaataca gctgacgccg
    13981 tatcatgact tgctactgtg ataaaggtca catctggcaa atcatatttt gaaaattgtt
    14041 ctttttgcag atctcctagt atcgttcccg gctctgtcaa tggtggaaat aattccttcg
    14101 agacaccgat tttttcaaga agttctattt cccattcttt tgtccctgca ttcagcagct
    14161 gcatagtaga tgcattagtt ttctccgtca ccatgttacc agtaaagaca taccctaaat
    14221 aatcaggtat cagtaacaat ttatgtgctt ttgccaaatt ttctttttct tcagcaaaca
    14281 attggaacaa ggtattgaaa ggctggatct gaatccctgt ctgctcatac aacttatcta
    14341 gagaatagat ttcagaaaac tgttctactg ctccattggt acgcttgtcc cgataagcaa
    14401 tcggatcatt tatccgatcc ccttgttcat caatcagaca gtaatcgact gcccatgtat
    14461 cgatccccgc aaaaaggcga tcaatccctg tctgctttaa tttttcaaat ccaatcaaaa
    14521 tctctttgat aagataatcg atcttccagc gatcgtgacc gtctttgttc cgaaagccgt
    14581 ttttgaaccg gtgtacttct tccaagatca aagaaccttt tgcatccctc gtacttttca
    14641 ttagtcgtcc gcttgatgca ccgatatcga tggcaacaaa tgttggattc atttctccac
    14701 ctcttttact ttattgttga ttagcacttt attaataaag cgttatcatt tattaacaat
    14761 gctatcataa tacagaatct tatattttgc ttcacactat tttggtatat aattgcatta
    14821 tcttgacaag ttgggagtgt ttacatggat tactgggaaa aagccaacca ctcatggtcg
    14881 gaagactcaa aaagagtgat cttgacgcct acacaaagaa gcaggaattt atttttttat
    14941 atccaagaaa tcggttactt caaagcacca atcccttact atactgaacg agcacattta
    15001 ccttcttact tgataaagtt tactttgtct ggcaaaggtt cgcttacata taatgataat
    15061 cagcaacagc taaaagctgg agacttattt tttattgact gccaagaata tcagcgttat
    15121 caaacgatca gcgaagaacc atgggaaatg gattggatcc atttgaatgg tcagaacgtc
    15181 caatcatttt atcaggagtt cacaaaagac ggccataaca ccttccattc aacaggggat
    15241 ccatcagaaa acaagatcca ccatttgatc acagaattga tccacatgca acaaggcacg
    15301 aatgcccgta ccgatttcca gtcctctgta gctatccacg agttgctgaa tgaactgatt
    15361 ttgcaaaagt atcagctaga tttcgaagaa gaggatattc ccgcctacgt ttacaaactg
    15421 aagcaatact tagacgaaca tttcaaagaa acgattacac ttgatcaatt agaacaacaa
    15481 ttcttattga ataaatatca gctgagcaaa gatttttcca aatttatcgg ttctccacca
    15541 atcgattatt tgatcagcaa aaaactttct tacgctaaag accttcttcg gtatacagaa
    15601 tttacggttc gaatgatttc cgaagaaata gggattgaga attttgctta ctttagccgc
    15661 ctgttcaaag caaaaacagg actatcgccg agagactttc gtaaattcag ctgattcata
    15721 taatatgtac gtcatttttt caaaatattt tgaaatatat agataaaaat aaaataaccg
    15781 cagtttctgc ttctatgtaa acaacagcag ctactgcggt tattttttag tgatcattct
    15841 cattgttcat cttcaaaatg ttcataatga accaactgtt tctgtgtatc acgtttcaaa
    15901 aattctttag gcgttatgcc gaactctctc ttgaatgctt tcagaaaatg cggataagaa
    15961 gaaaaaccac ttttactata tacagcacta gagctgcggt attctcgcaa caagtgcttg
    16021 gcatagatca gctttttctt cattacatac tgatgaaacg taaatccagt gtacttttta
    16081 aattctcgtg tgatatggta tttgctgaca aaaaactgct tttccatttc ctctaaagaa
    16141 agcggatgag ataaattttt tgagatatat tgtaacattt gttccacacg ctcattagga
    16201 actacagcat tctgttcttg cgcactctct tctttttgta caacttgatt gagaaaaatc
    16261 aagtattcta ccagagcttg ttcatatttt atgtcttcac cgtaaatttt tttatcagga
    16321 ggatggtcga taaatgataa tttttgcttc aatacttcct gttctatttg caatacacga
    16381 ctcctcttct ctcccattgg ttgaaagcaa gccgatagat tagaccatgg cgtggaacga
    16441 ctttctagaa aagaaggggt gacaaagata tagacacgct cgaatacatc tgaagattgg
    16501 cggacgatac gatgcaaatc attggaatgg atgagcagca cacttcctgc tttgatcgta
    16561 aattgtcgtc cgttcaaata aaaagtcgct tctcccttta atgtagcatg gatctcatag
    16621 aaatcatgat gatgatagat cgttttattt tggctgacga catctttgac atgatagatt
    16681 tcatactgat cagaaaccat cgtattgact tgttcaccgc tgattttctt ttggagcatg
    16741 tgccgcacct ccttttgttc acttatgcac aattttatta taccacgttt tttgtaatca
    16801 aaaagagctg cttttatata ctcttataaa aaagaagaga cgtggcatct tctagccccg
    16861 ccccctccat tctaaatatt agttaaccaa cgtcttcttt acattattca agaagacttc
    16921 ttccataacg aatcccatca tcttttgttt cttcaagaac cacatacagg tacggacgat
    16981 tcttcatgat gatttctttg atttttgtgt actcgatcac accgtctccc aaatttaccg
    17041 gcacgatttt ttcctgttcg acacgaaaat ctttcaaatg aaccgcacat atcttttcac
    17101 caaaacgttc aaaagcttct tctaccaatt ggacttgttc ttgataatca ctacttgtga
    17161 tcaaattggt cggatctaaa atgattccta gatagtccga atctactgct tggatcagct
    17221 gctctaccct tgctaaagaa tacaccggat gattcaaacc tggttctatc cccaccatca
    17281 tttggtattt ttctccagct tttaccaagc gacggataac ggacacagct tcagcaaatg
    17341 cttcatctgt gaaattttct tccgtatatt ggatttccgg caaaacacat ccagtttccg
    17401 aagcaaccat cgacgcacca aaggaagaag catagcgaag atactgctcg aactttgtca
    17461 gcaactcttg tcgtatcgtt aaatctggat gaatcatatt gatatagcaa cttaaaattc
    17521 ctacggtcac ttcttgcttt tctaaaactc gtttgacata atttcccatc ccagaattga
    17581 tctcatttgc gccagaaggt atttcaggaa acgaaagacc taaggcgagc tgcagtgtat
    17641 gaatcccttg ttcttttgtt ttttctgcaa gatcttctat cgtttggact tcggaaagat
    17701 catgtcctct aattgtaatg ttcaacataa ctattctcct aactcagcat attgggtagc
    17761 cacatgacga tttgcgggaa gtaagtaatc aaaaacaaga cgactaagat aaccccataa
    17821 aaaggaagaa gcggtttcag cattgcctct gcttttacgc ctcctacact tgcaccgaca
    17881 tatagtcctg tacctactgg cggcgtgatg gagcctacac acagattcat gattgaaaac
    17941 aacccgtaat gtacaggatc aacccctaca cttgtgacga ttggtaaaaa tattggtgta
    18001 aaaatcaaga tagcaggcgc cacatccata aacataccca ccagtaataa cacaagattg
    18061 actaataaca gaataataaa tttattatcc gttacgccca taatcaacga actaattgcc
    18121 tgaggaatac cggtaaacgc catggcgaaa gacatcatgg aagaagtagc caataagaac
    18181 atgatcgtcc ctgacattgc tactgattga aacaacatat taggtatatc ttttagtttt
    18241 accgttttat agtaaaacat agcaatcaac agggagtaaa tgacagctat cgctgaagct
    18301 tctactgctg taaatatccc cgtcaagata ccaccgataa tgatgacaat caatagaagg
    18361 ctaggaatcg cctcttttat tatttttcca gcatgagcca tctgtccttt ttcgacaaca
    18421 gggtatttat tctttttagc aacgatgaat gctactagca tgattcctag cgcccacaaa
    18481 gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc agcaacaagt
    18541 gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc tgacgcgata
    18601 ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg gatcaacact
    18661 ccgccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa taaagcattc
    18721 cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa
    18781 tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag aacgaaaaac
    18841 ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc tgaactaaaa
    18901 gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa tgcaatactt
    18961 atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa cactaatccg
    19021 gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc tggcttgttg
    19081 cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc cgatacaggt
    19141 actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg ccccattgtc
    19201 aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac cagaaataaa
    19261 ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc acttaaaacc
    19321 gttacggcta aatgtgcttt ttgtcccaca acatacgcgg aagacaacat ggcaaaccag
    19381 acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa aaccgttctg
    19441 gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc tcccaggatc
    19501 tctaatacct tgtttaaaat cattttcatc acagctacgc tccttgctct tggatctttt
    19561 gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg gcaactgcct
    19621 ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct tgtgctaaag
    19681 ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg
    19741 ctgcttcttc aatggctttt tcctgctcat cagttagctg ttctttgatg gcatcattca
    19801 tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta gccacaccac
    19861 catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc aaattggatt
    19921 gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct aaaagctcta
    19981 ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc aaatcttccg
    20041 gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca taataggtca
    20101 atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt tcttcattat
    20161 ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaactgaa aaaatcgaat
    20221 aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct actgctcctg
    20281 actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg aaatactgga
    20341 tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc attgcatcaa
    20401 ttactggctg gctgttgctt gcataagcaa agcgcaaggt aactatctca tttacatctg
    20461 catctgcgac agttgccaca ctagaattat ttccgcagcc gcttagacct agcagaccta
    20521 ggctaagcga acttgctgca aacagcagtg atgttttttt catatgggtt tcctttcttt
    20581 taacaataag agacgagcaa ttctgtcaaa caaagcaccg ttaacgactg tccataaggc
    20641 attgctgtga ttccgatatt ccgataaaaa tctaaactgt cccccattcc tgtcccgatt
    20701 gaaacgtttt gaacttctcc tttttcatcg atttgttcaa gcaatccttg gatagcacga
    20761 taagcgactt ctttatattc ttgtggtaaa taacgtttat ggaccgcttt caaaataccg
    20821 taagcaaatc ctgcagttgc agaagattct acgtaagaag aaggatcatc caacaacgta
    20881 tgccataaac cgctttcaga ctgataagtt tttaatgcac gcacttgtgc ttcaagtgtt
    20941 tcgatcaata atttgcgcaa tgcatcttct ttcgataatt ctaagatttc aatgatctct
    21001 ggaatagcaa tcgtcaacca gcagtttcca cgagcccaaa atgcttcggc atagttatga
    21061 tttccttcaa acgtccagcc gtgataccac aatcctgttc gtttatccat caagtatttg
    21121 atatggatca taaactggta acgagcttct tctaaatatt ctgggcgatt caacagtttc
    21181 ccgatttttg ccaaaggaag cactgtcatc atcaatgtat cgtcccacaa ctgattctta
    21241 ttttccggtc catatgtggc atgctgtaac ccgtcttcat ttgtccgagg catgtcattc
    21301 atgacccatt ctgcccattg ttctaaataa ggcaaatagc gtgaatcttt cgtgtcttca
    21361 tacaagtaag ccatagtcaa tagcggtgcc attgtattga cgtttttggg cggagcgcct
    21421 tcttccatac gatcctcaaa ccattcattg accacttgat aagcgcgagg attttttgtc
    21481 atctggtaat ttttataaat accatataga ccgacacctt gtggccaatt ccacactgtc
    21541 cagcttttgt catctacttt cagtccatca aagtccaaca aaaattcacc attatcgtct
    21601 ttgatcgttg tcaggttttc aacgagcagg tcaatttttt ctttcactaa ttctttaggt
    21661 acaaaaatct cttctttaat aatcatcttt acgccacttt tttaaacgct ttcattttat
    21721 atccttaata tagcagtaat accgtccact ataaatatcg ctttttgaac aaaaactgtc
    21781 taaaattgac ctaaatgtta aataacaaaa gtaactttca gttacttgga aattaaaaaa
    21841 agaacttgga gaaacaacgt ttt
//
LOCUS       DAFELO010000045        21612 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFELO010000045
VERSION     DAFELO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.045"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..204
                     /locus_tag="LP452_001841"
     CDS             <1..204
                     /locus_tag="LP452_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014566.1"
                     /translation="IESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVHDFEES
                     KAVEFEIVKKVMELIEKYEEDSE"
     gene            225..314
                     /locus_tag="LP452_001842"
                     /pseudo
     CDS             225..314
                     /locus_tag="LP452_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            389..856
                     /locus_tag="LP452_001843"
     CDS             389..856
                     /locus_tag="LP452_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBL2014567.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            1131..1367
                     /locus_tag="LP452_001844"
     CDS             1131..1367
                     /locus_tag="LP452_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014568.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1391..1732
                     /locus_tag="LP452_001845"
     CDS             1391..1732
                     /locus_tag="LP452_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014569.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1725..1916)
                     /locus_tag="LP452_001846"
     CDS             complement(1725..1916)
                     /locus_tag="LP452_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBL2014570.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            2126..2773
                     /locus_tag="LP452_001847"
     CDS             2126..2773
                     /locus_tag="LP452_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014571.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            3033..3377
                     /locus_tag="LP452_001848"
     CDS             3033..3377
                     /locus_tag="LP452_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL2014572.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3382..3663
                     /locus_tag="LP452_001849"
     CDS             3382..3663
                     /locus_tag="LP452_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014573.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3766..4080
                     /locus_tag="LP452_001850"
     CDS             3766..4080
                     /locus_tag="LP452_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL2014574.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            4058..5752
                     /locus_tag="LP452_001851"
     CDS             4058..5752
                     /locus_tag="LP452_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL2014575.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYNIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5772..6950
                     /locus_tag="LP452_001852"
     CDS             5772..6950
                     /locus_tag="LP452_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2014576.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            6913..7599
                     /locus_tag="LP452_001853"
     CDS             6913..7599
                     /locus_tag="LP452_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL2014577.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7599..8759
                     /locus_tag="LP452_001854"
     CDS             7599..8759
                     /locus_tag="LP452_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL2014578.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGECVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8769..9644
                     /locus_tag="LP452_001855"
     CDS             8769..9644
                     /locus_tag="LP452_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014579.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCFYAYVKAIKAVVR"
     gene            9641..9952
                     /locus_tag="LP452_001856"
     CDS             9641..9952
                     /locus_tag="LP452_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014580.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9942..10295
                     /locus_tag="LP452_001857"
     CDS             9942..10295
                     /locus_tag="LP452_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL2014581.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10285..10686
                     /locus_tag="LP452_001858"
     CDS             10285..10686
                     /locus_tag="LP452_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014582.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10679..11083
                     /locus_tag="LP452_001859"
     CDS             10679..11083
                     /locus_tag="LP452_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014583.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            11095..11700
                     /locus_tag="LP452_001860"
     CDS             11095..11700
                     /locus_tag="LP452_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2014584.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKNSVVTTTATQSQ"
     gene            11720..12082
                     /locus_tag="LP452_001861"
     CDS             11720..12082
                     /locus_tag="LP452_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014585.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            12085..12267
                     /locus_tag="LP452_001862"
     CDS             12085..12267
                     /locus_tag="LP452_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014586.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12284..15703
                     /locus_tag="LP452_001863"
     CDS             12284..15703
                     /locus_tag="LP452_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL2014587.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15754..16491
                     /locus_tag="LP452_001864"
     CDS             15754..16491
                     /locus_tag="LP452_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2014588.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            16501..18792
                     /locus_tag="LP452_001865"
     CDS             16501..18792
                     /locus_tag="LP452_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2014589.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18816..20906
                     /locus_tag="LP452_001866"
     CDS             18816..20906
                     /locus_tag="LP452_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014590.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLIANINADSFS
                     QGTGALSVIDDGDEVVVTLDPNHKLEVFKAKSQPDLLIGKTYTMSVEIMLEDDFTGDP
                     SKIGLRYIKMPNWTSELYTRNALTTAKGVWQKLTGTVKITATSDNAESWLIMLQNKDA
                     NNSLSGKLRLRYAKLEAGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            20923..21072
                     /locus_tag="LP452_001867"
     CDS             20923..21072
                     /locus_tag="LP452_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014591.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            21069..21515
                     /locus_tag="LP452_001868"
     CDS             21069..21515
                     /locus_tag="LP452_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014592.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21517..>21612
                     /locus_tag="LP452_001869"
     CDS             21517..>21612
                     /locus_tag="LP452_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2014593.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7696 a         3207 c         4500 g         6209 t
ORIGIN      
        1 atcgaatcag gagcaacact atctgtggaa tcggaccatc taatcataaa ttttggtgga
       61 aagcgccaga ttattttgtg ggtagttgat gatgtactgt ttccagaaat tgttcatgat
      121 ttcgaagaat caaaagcggt tgagtttgaa atagtgaaaa aagtaatgga attgattgaa
      181 aaatacgagg aggacagcga atgataccga agtttagagc gtgggataga gaaaaagaca
      241 gaatgaaggc agtagctttg gttgaatttg ctgaggcgta taaatcagga gaactcattt
      301 cacgtacaaa ataaaaaaag tcggattcct ccgactatga ataatatttc cgacataagt
      361 attatatcat aattggggga atcagaggat ggtacttttc gatgtaaaga aatatgagac
      421 accaagcgcg aaagatgttg atatggaacg cacaaaacat aatgtcgctg tgtttctttc
      481 agcatatcta tcagctagat gtagagtagg gcaacctcga gaaccaaaag tgacagcatc
      541 ttattccttg gttccacctt ctacagctga tcatatattt gaagcagaaa gaatgctgat
      601 cgataaagaa gaagcacagg aagaatttga gtatctgcat aaattgtttg ttcgaggcta
      661 ttcagcgata cagcatccgc ataagcctga tgtgactgaa aggcgcaaga agatattcta
      721 tgatcgttat atcaatggtc tgcccattta tgtaactgct caaaggaata atactagcga
      781 agaatcggtt aaagtagaat caaacagaat tatcatccaa tttgcttcat cgttagaact
      841 ggttgctttc aagtagccag tttttacact ttttataccc ttttattacc aatttggttt
      901 ccattttata ccttttttat accaatcact tacctattca acgttgtatt atgatagtgt
      961 cgaaagatta ggaaacagga tcgacaaaat aaaatgtaag ggaggaaatc tccctcatcg
     1021 tttaattaag cttcgatagg cagcagcgga aatattaaga ataaggatgt gaatttcaac
     1081 tccttctaaa ttgttcttat tatctatcat ccgttgctgt ctattaattt atgtattgga
     1141 gggtatataa aatgaagatc gcttccgaaa aaattgatca actaacaatt ttagtaaacc
     1201 aaagaatcga agatattatc aaagaattcg aaggtgaaaa gattaagaac ataaagctta
     1261 ctttaatgga tcataagttc gaattaaatt cgacaaaaag gaagatcgtt gagcttatag
     1321 aagaagaaat agctctccag atcaatgagc aacaattagc taattaattt atgtattgga
     1381 ggggaaatga atggaagtcg aacaaataaa gtatcaaggt atggatcggg aacggttgat
     1441 tgatattatt gagcaacaac gtttagaatt gatccaaaaa gaagctgtat gtaaagagta
     1501 caaaaaacat ttggaacaag taattgagta tcattcagta gaaaagtaca gatcagtggt
     1561 gcaaaaaaat agagcaacga gtcctgattc tcgtcaagat gtatcgatta gtatcaagac
     1621 gccagtattt aattctgtta aatcagaaaa gctagaaagt atctcaactg ctttggtaga
     1681 aaaagcagct gaaatgaact ccctagcaaa tgaagttagt cgtcttattt agtcatatcg
     1741 ataacttgag agtctttggt gattccgttt tttatagaat taatggtttg ttcagcggaa
     1801 tatttagtca agtatgtttc gctagttgct actacttcat tgttattgga cttaataacg
     1861 aagtaatatt ggccattagt tgcttctctt ataacaaagt acaaattttt caccacctat
     1921 aatttatttc agcggaccac tcgctgataa ttaaaattat aagctatgta taaaatttca
     1981 caatatcaat ttgtcgctgt ggcggaaagg gtagacgcaa taaaggatga atgggaaaag
     2041 tcaatgagta attatctgaa atttacaagt cccctgttta gttatctaag attatttgat
     2101 aatattattt ctgaggtgat agaaaatggt gggcgtagtt aaatttcttg ggtttacatt
     2161 tgatggatat ggtacgttag gcgaatgggt aggtattatt ttgatagtga ttacaattca
     2221 acaaacatca aagtactact ggtattctaa tagttctaaa attgtatcaa gaataactga
     2281 aggaataacc aaaaaagata ttactgtcgt cggtaaatat gggtttacta taactttatt
     2341 aaatgaaggg aaatctgaaa ccgcaataaa gttttgcggg attataggta aatataacat
     2401 tttccaacgt tttatcagaa agcattggta caaatatatt tacaaatacc ctgtagttaa
     2461 aaagcttatt gaaaaatatg tggaccacga aatatcttat ctcaatccaa cagaccttat
     2521 taatcaagaa ttccagaata ttggatactt agaaaaaggt ctaaaaatac caattgtaaa
     2581 tgaaagttta gttactgcaa tggtagctaa cataaagaac agcaagaaat tagaaaagag
     2641 atataaaaaa tgtaaaaaat tgaaattttc ctttttattc ttagaatata atggcaaaga
     2701 atattatcaa gatatttatg ttttgcgagg ctcgaactta tataaagaca ttaaaaataa
     2761 gatttcagta tgagattttt taaaattcgt agatcactct ttgagtggtc tttttatttt
     2821 gcgtaaagga ggcacgacga tgtatagacc acaatactta gaacagaagc atgaagtaat
     2881 cactgtgcaa aacggtaacg gtgagatagt acgaaagtat agaagaccaa taaagagcga
     2941 tacatataaa cggaaggaaa gcaatgaagt tattccattg tatggcaaaa gaatagctaa
     3001 gtattaaata agattgcgaa aggagatggg acatgaccga ggaattctat agatggttat
     3061 tacagttgat aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     3121 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     3181 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     3241 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     3301 gatatcttac agcgttagat aaacaagaga agaagataga aagcttcgct aacttcgatg
     3361 caagtgaaag gtggtaagtg catgatcatc aatgacaatg gcagagagta tgatacagaa
     3421 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     3481 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     3541 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     3601 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     3661 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     3721 aggaaaagag actttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     3781 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     3841 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     3901 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     3961 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     4021 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     4081 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     4141 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     4201 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     4261 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     4321 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     4381 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     4441 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     4501 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     4561 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     4621 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     4681 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     4741 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     4801 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     4861 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     4921 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     4981 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     5041 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     5101 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     5161 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     5221 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     5281 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     5341 tgaaatgagt aataaatata atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     5401 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     5461 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     5521 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     5581 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     5641 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     5701 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     5761 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     5821 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     5881 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     5941 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     6001 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     6061 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     6121 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     6181 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     6241 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     6301 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     6361 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     6421 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     6481 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     6541 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     6601 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     6661 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     6721 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     6781 gcattagaaa tggagtattt acaatcaacg aaggaagaga gcgcgttgga aatgatcgct
     6841 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     6901 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     6961 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     7021 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     7081 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     7141 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     7201 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     7261 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     7321 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     7381 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     7441 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     7501 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     7561 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     7621 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     7681 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     7741 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     7801 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     7861 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     7921 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     7981 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     8041 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     8101 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     8161 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     8221 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     8281 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     8341 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     8401 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     8461 gagaatgcgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     8521 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     8581 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     8641 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     8701 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     8761 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     8821 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     8881 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     8941 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     9001 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     9061 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     9121 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     9181 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     9241 acactaattc aggaggcgca cagtgggtta ttaaccgccc taaagcgaat tattggaaaa
     9301 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     9361 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     9421 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     9481 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     9541 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     9601 gcttttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     9661 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     9721 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     9781 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     9841 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     9901 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     9961 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
    10021 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
    10081 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
    10141 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
    10201 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
    10261 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
    10321 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
    10381 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
    10441 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
    10501 ttctattcag aaaatagaga agatttaaca ggagattcac tagatattat ttcattgatt
    10561 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
    10621 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
    10681 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
    10741 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
    10801 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
    10861 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
    10921 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
    10981 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
    11041 tacagaactt ctgattcgtc aattcgataa attaaataaa taggaggaac aacaatggct
    11101 aaaacaacaa cagtaacaac ttttgacaat ataagtatta aaagaatttc ttttaatttt
    11161 aagaatgcaa caaatgcaat ttcaactgat tgtaatggac aattagatgg cgaaacagaa
    11221 atgcagagaa ttgtaaaaaa atgcggttca acagaagtaa aatcgaaatc taaaccaatc
    11281 aatatgacgg taacaattac tgcacatgta ccgatggaag tttatcgacg cttcaatggg
    11341 ttgaaacaag atgaacgtat taaatcggga atttactctt atggtcctga ttccgtaggg
    11401 gaagatttct ctcttgctgc agagatcgtg gatgatttcg aagaaaaaaa taagctgatt
    11461 ggtatgttag catgcacttc gaatacagga ttaacattct ctattgaaaa tggtgcggat
    11521 gaagtagctg cgttagaact agaaacaaaa gttatgcaag atgaatttgg taaattttat
    11581 catgaagcaa ttgttgcaga acttgaagaa gacttaacag atcaatggat gacgaatcta
    11641 tctgctgatg tgattaaaaa aaattcagtt gtgacaacta cggccactca atcacagtaa
    11701 aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa attgaactaa acgatggaac
    11761 aattttgaat ttagaaccta aactgaatat caagaaatta ttgatgatca atagagattt
    11821 taacacagac gagtttgcaa aaatgactgt gggaaaagga tccatggata tttctgttat
    11881 tcaaggtgca aaggctgtgt atattgctta ccgccaagcg aacatgactg attatatttc
    11941 attcgatgaa tttatcgata aatgggattt tgatatggct actgccagct atatttatca
    12001 attgatgatg ttcaaacaag cacgcgatgc ttatcaaaaa gaatttgaaa aagcaaataa
    12061 ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag ttgaaacgtg ggtcgatgtc
    12121 tattcgatgt tgaccgacgt tttttctatg ccttcagatt tggttttaag cgatatctgt
    12181 ttagatgaca ttttgcaaat ggcttacaac aagagtgctt atgaaggatg gaaaaactat
    12241 gcaataaacc aatcccagaa aaactaaaga aaggaggtaa aaaatggcta aaaagagaac
    12301 agaagcagaa gtaacattca tagctaacga tgacggattg aaatctacgt taaaagaaat
    12361 cagtgctgaa ttaactaaaa atagagcaga attaaaacta gaacaagctc aattacaaca
    12421 gactggttct gaatcagaca agttaggaag taaattatct tctttagaaa agcagtatga
    12481 attacaaagt caaaaagttg aagtaactag ccaacgttta gccaatgcca aaaaatatta
    12541 tggagaaaat tccaccgaag ttcagaaact tgagagagaa ctgattaacc aacaaacagc
    12601 gcaacaacgt ttgtcaaacg aaattgataa aacgagtaat gcactagctc aagcaaaagg
    12661 cgaaatacag acgtacgagt ctacaatgca acagttggat agtgaacaaa aaaatgttca
    12721 agctagtgct tctctgattg aatccgaata caaaaaatgg caagcaactg ctggtcaatc
    12781 agcttctgaa tccgagaaat tagcgaaagc ccaagaatat gtttctcaac aatctgaaaa
    12841 tgcagagaaa acgatagata tcctgagacg acagttagaa gctacacagt ctgagtttgg
    12901 cgctacatcc acagaagcaa tgcagatgga ggcgaagctt aatgatgctg aacgtgaatt
    12961 tgaagagtta ggacaagctg ctaaaaatgt agatacaact aacttggacg atatcggaag
    13021 caaaatagat atgaataatc taatggaagc ttctgacgtt ttaagcgaca ttggcgataa
    13081 gcttacagaa ttagggaaac aagcagtgga ctctgctaat agtgtaggta gttcccagag
    13141 taaaatacaa gctaattttg gtttgactaa acaagaggct gaagaattaa cgaatgtagc
    13201 cagagacatt tattataaag gttttggaga atcgttagat cagtccacag atgcattgat
    13261 tttggtaaag cgtaatttag gcgatttaaa taatcaagat ttacaaaata tcacggaaca
    13321 agctatggtc ctagaaaaca ccatgggcgc tgatatggat gaaacgttac gtggtgtaaa
    13381 tggcttaatg gtcaatttcg gcttgagcgc tcaagatgca atggatttaa tggtttcggg
    13441 tactcaaaac ggtttagata aaacgcacga attaggcgac aatatggcag aatatagcca
    13501 attatggagt caaatgggat attcagctga tgaaacgttc ggaatgcttc aaaatggttt
    13561 agatgcgggt gcttataacc ttgataaagt caatgactta gttaaggaaa tgggaatatc
    13621 gttaacagat ggtcgatttg agcaaaacat ggatatgttt agtgaaagta ctagaaaagc
    13681 ttttgaagag tggaaaaatg gcggaggaac acaaaaagac gttattaatt ccatgattca
    13741 agattttagc aatatggatg gtcaatacga ccaattaaat aaagcttcta caatttggtc
    13801 tgcacttggc gaagataacg cgatgaaagt tgtccaatct ttgactgatg ttaaccatac
    13861 atttgatgat gttagtggat ctgcacaaaa aatgaatgaa gattctacta ctccgttaca
    13921 agaattaaac ggaaaaatag ctgaattaaa ggattcatta gctcctatag gcaacacaat
    13981 catagatgca ctcgaaccag taattgattt tctaggaaag atggctgatg cgtttaataa
    14041 tcttccacaa ccagtacagg attatgccgt agcgattggc ggattgactg ctgcatttac
    14101 tttattaatg ccaataatag ttggcttcat ggctctaggt ggtcctacta cattaataat
    14161 aggagcagtt attactgtta ttgctggagt tatagcaatt ataaaaaact ggggcgcaat
    14221 tactgactgg tttaagggaa tatggagtaa attcactgat tggttgggtg gtacttggga
    14281 aagtataaaa gaaggtgcct catcagtttg ggatggagtt aaagaaacct ggtctggatt
    14341 tgtagattgg gttcaagata tttggcaagg agtttctgat tggtttggag agctatggag
    14401 cggattagtt gaaggagctt ccaacatctg gcaaggagtc caagagactt ggcaagcatt
    14461 cgttgattgg gtttcaaata tttggaacgg agtcaaagaa gtatggtcga ttatttgggc
    14521 agacattgta ggaattgttc aaataccatg gaccttaata acgtcattga ttcaagccgg
    14581 tattaatatt atcgtgggta tttttgatgt agctggacag ttattaggcg cagcttggca
    14641 agctgtttgg acacctattt ctgatttcct taaaaacact tgggatacta tgacacaatg
    14701 ggtaagcatc gcttggaatg gaattgtaac tacattccat actatatttg atccagtagt
    14761 ggcatggtgg aatggtatat ggacagctat tagtactacg gcttcaaata tttggaattc
    14821 aattagtgca acagcttcta gtatttggaa cagtatcaag aatacaatca ctagcttggt
    14881 acaagcagct gctacagtaa ttcaaaatat ttggtcaact gtatctagtt ggttaggtgg
    14941 aatttggaat tcaatcagct ctacagcatc aaatatctgg aatagcgtga ctagtagtat
    15001 aagcaatgct ataaacgcag ctaaaagtgc cattcaaagt gtttggaata gtatatcttc
    15061 gtggatcagc ggaatttgga acggtatcaa aaacactgct ttgaatcttt ggaatggaat
    15121 tacaagcact attagctcta aagtaaacga tggaaaaaat gcaatttcaa gcggttggtc
    15181 caatctaaca ggtattgttt ccgacatatt caataatgtt aaaagtacaa ttgctaacat
    15241 ttgggaaggt atcaaaaaga ctgttagcgc tccaattgat tggatcagag ataaaatcag
    15301 tagtatcttt gataatttga atatttctat accacatatt ccgttaccac attttaaatt
    15361 gagcggagaa ttcaatccat tgaaggggaa aatcccaacg ttgggtgttg attggtatgc
    15421 gaaaggtagt gtgtttaatt ctccgaatat tatcggtgtc ggcgaagcag gacctgaagc
    15481 agttttacct ttgaaaagat ctgtgctgca agaaattggt gatcgtatct tgagtagcac
    15541 atcagtttca tctagggcac aaacgattca acctgtgaat aactacgaat tcaatttcac
    15601 aattgatggt aacgcagatg aggttactat gaagcaaaca actcaacaaa tcattgatag
    15661 cattacaaaa gttcaaaatg ataatgcttc ggcatggcgt taaacaggag agtatttctc
    15721 ctgttttttt agtattaaaa aggatgtgaa aaaatgactg attgtataca ttctataatc
    15781 gatggatttc ctgattattt gcataaattg gctttagctg aaagaccaac cataccttct
    15841 ccaaaaagac agagagttga aacttctgtt ttaggaaggt taggtggctt agtacaagat
    15901 tactcgtttg aagacatgtc gtttacattg cactataact atttagagga tgtggaagac
    15961 catcaagcgt tcaagcaatc gttttatatc atgcgtcatt ggttaaatta tgcaaagaaa
    16021 ttagaattct ctgatgatcc caacgtctat tatgttatcc agactatcga tattggggat
    16081 gcagaaaacg atattgttga atggggagag ttcgatgtaa atattactgc gaaaccattc
    16141 gcaagagttc aagaagatgt acctataacc gtagataaac cacagtcatt taacttgctg
    16201 aataatagtt tagaagaaag ttttccaaag attatcatca ctccttcagc tacttcatgc
    16261 cagttcatct taaatgatta tgtgtttagt tttgaaggct tagtagtagg aactgacgta
    16321 gtcattgata gtgatttgat gctttgctac gaagagcaat cggacggaga tattttagat
    16381 cggtccaaca aaatgaagac catgcaatat ccgacattgc aagtggatat taattatttt
    16441 aattgtactg gtttgagcaa aatacaaatt tatcgtaatg ggttaaggta ggtgaaatag
    16501 atgatcgata atttaataac tatttacgat aaaaacgacg cgaataattt agctgaacat
    16561 ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac tgttagcaat
    16621 aaattaaacg gagccgagat atttcagggt acttatccaa taagcggaac taatgcagat
    16681 ttgattgtag aaggacgtat tattcagtgt tatgtagatg aaaatcgagc aaaacagcgt
    16741 ctacggattt attatgcaaa gacttctgta ataggcaata cgatagaagt aaaagctgaa
    16801 cctattttca atgatataag aaaatcggtg ttgaataaat atgacagtgg aacagaaaag
    16861 atcactgcta gtcaggcatg gcaaaacgca aaaactttag cgaaaccagt tattccttca
    16921 cagttttctt tctcgtcatt agtagatacg cttgctaatg tgaagataga aaaggcgaat
    16981 tttttagaat tctttggtgg aaaagaggga tctattctag atcgatttca tggggaattt
    17041 ctaaaagata ataacacatt acgtcatgaa aaaaggctag gcacggatca taaaatcaaa
    17101 gcgatttata ctaaaaactt aactggtctt gacttagaga ttgatgctca aagtgtttta
    17161 gttggagttt atccattcat tagcagctct tcagaaggag aagacgagat cactctacca
    17221 gaagaagtta ttttcacgga ttacgtggat gattatcctg ctggatatgt ttcttttgtt
    17281 gattttaaag acaaagcgac tgatgtagcc acattaaggg aagctgctaa agactggttg
    17341 aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt cgattgaatt agtaccattg
    17401 aggcatcaaa gaggctatga aaaatttgtt gatctagaaa aagtttcgat gggtgacgga
    17461 gtagatgtgt atcatccaca gttaaaagtg aatatgtcag cgagaattgt ggaatatacg
    17521 tttaatgttc taaccaattc atacgataaa ttagttgtag gaaacgtcaa aacaaacttc
    17581 ttagaaaaca cagagaataa tgtcagcaat ttgattaatg atgccattga tcaattgaaa
    17641 aacggtggcg aaatcagtga tttactcaat gatattgtag atcatcaaac tgatatgatt
    17701 actggtcaaa atggtggtta tgttttatta gatcctaaag aagcgcctag tcgtattttg
    17761 attatggaca caccagataa gaataccgca aggaatgttt tacaaatcaa caatgctggt
    17821 attggttttt ctaaaactgg cattaatgga acatatgaaa cggcatggac gttagatggc
    17881 ggattcaatg cctcgtttat tacggctggt gaaatagtag gaattactat tagaggtact
    17941 acattaatta gtgatggtgc tgattataga acaagtattg ctaatggcaa aatgacttgg
    18001 tactcaaaaa aagttaacaa agatattatg gagctagaag cacgtgatta tgtaagtgct
    18061 gatgccggta ttgtatcata caccatgaaa actggtggtg gtttcatgat tagaaatcca
    18121 cagggtaact tggtttttag tacgtgggat aatggtaata acagaccgtt tctatctttt
    18181 ggtgcgccca atttcaggta tagcaatgct agttatgtaa cttctggcga cggtagttct
    18241 ttaagcatta atggtagtgc gggtaactca tgggaattta aggtagctgg taggactatg
    18301 aaatttacta gtgatggtat gctaacgtta ccaggttgtt tttttggttc atgggaagat
    18361 gggaaacttg ctaggtttga acaatcaacg gtacaagtat ataaagattt tactgttaga
    18421 ggtactaaaa actcaactgt accaacagaa cattatggac aacgactatt gaacgcttat
    18481 gaaactccag aatattattt cgctgattat ggggaagccg ttacaggtga cgatggtaaa
    18541 gttcgtgttg atattgaccc catgtttgct gaaacagtaa atctaagtcg gtatatgaca
    18601 catgtgacac ctacagaact agttttgtgt gctgttactc atgaagatat tgaccatttc
    18661 atcattgaaa ctagtaagcc aaacgtatta gttagatgga atttagtggc acaccgtcta
    18721 gggtatgaag atattagatt aaaagaggat acagcatatg atagcacagt gcttgaccaa
    18781 aaacgttttt aaaacgaaga caaggaggta tataaatggc tagcagttta tataatttgg
    18841 ctttagattt cagcaaagaa ttaaactaca ccaaagctat tatggctcgt cagggtgata
    18901 aagggattac ggtgacggtt aaaccgtttc taaatggctt gcagatggat acgagtggcg
    18961 gaacatttac tttaaaagga acaacaccat ctaaccgtta cgtagataat gttgcaacta
    19021 gtgtaactag tgaagaagtc acgttttctc ttgatggcac atttatgagt gaagcaggat
    19081 attataaaca ctgctacgta gaatatagaa aagacaatca aattttaaca acgcaagata
    19141 tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat gaatatgttt
    19201 cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt atggctgaaa
    19261 tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt caagctaaaa
    19321 cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt aacttacaag
    19381 tgatgtacag caacagcatc gactttgggg gctatgatta ttcggggaat ccgaatttaa
    19441 tagccaatat aaacgctgac agcttctcgc aaggtactgg tgctttatct gttattgatg
    19501 atggtgacga ggtagtggtc acgcttgatc caaaccataa attagaagta ttcaaagcga
    19561 aaagtcaacc agacctgtta ataggcaaaa catataccat gagtgtagag attatgttag
    19621 aagatgattt cactggagac cctagcaaga taggcctaag atatattaaa atgcctaact
    19681 ggacatcaga actatatacg cgtaatgcat taactactgc taagggtgta tggcaaaaac
    19741 taactggcac cgttaaaatt actgctacga gtgataacgc tgaaagctgg cttataatgc
    19801 tacaaaataa agacgctaat aacagcctat ccggcaaact acgtttgaga tacgctaaac
    19861 ttgaagctgg ttcaacagcc acaccgtacc agcctaatct acttgatgca ccgtattatt
    19921 tgagtaaggt ggctttgggt gagaatctaa ttaaaccaga atcacaacaa ccagttacta
    19981 atagtaacta tcttattaac acctataaca ttaaaccaat ggtaaaaggt aagaagtata
    20041 ccatcacact tgaaggaact aagccagcaa cacaggtttt tagaccattt ttcacacgag
    20101 caacaggaga tgcatgggag gttggtgact tacaaccagt agaaggctta actaatgtgt
    20161 ggtctaaaac atttacagca gcagatgact cacaccctac taccccacaa gtgcagattt
    20221 atcaagtacc aagcacaagt gtaggacaat gtacaattaa gtggttaaaa ctagaggaag
    20281 gcaatactcg aaccccgaat attagtgagt ataaatatcg tggtactggt atgcgtgatt
    20341 caaacaatcc aaaagattat gtttgggatc tagcaccaga atatgtcgaa gataatttgg
    20401 ccacagatat taaaatttct gaaattactg gtaaagcaaa caattatacc gatgggaaag
    20461 tatcggagat taattcgcag ttgactgctt caattaatga agtagacacc acagctaagg
    20521 atgctcaaac aaaagcgaat gctaatgcga ctgctataga tgaattagac aataagatcg
    20581 atgaacgcat taatgataca gctactacca cattaacagt tacaaacggg aataccggat
    20641 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
    20701 aacgcagtgg tggaaatgat tcaacgatac taacaattcc agaaggctat cggccaccaa
    20761 ttagttttga gcaactggtt ggctcgatag accgttctac tttgaacagt gctcagttat
    20821 ctattggtgc agatggagcc attaaatggc gaagaaactc aagttatgga tcggattata
    20881 cctttgcaat tacttacacg atttagaaaa gcgtgaatcg atatgaaggc agcatataga
    20941 ccaattgaac cttacggatt cgagcaaatc attgtgaatg atgaagaaca tttaccggaa
    21001 gaatgcacag aagtcgaacc accgattcca aattggaaac cgaaattcaa ttactgggag
    21061 ggaaataaat gaaaaacatt tggaaatatg gacgtactgg cggagagtac gcaggaaaag
    21121 tattggacga catgcttgta tccgttcctt acacagatca gccaccgctt gaaggaattc
    21181 gtgctgatgg cgaaccgtta acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat
    21241 ggattatttt agcgaacgca ctagatcaca acgatttaaa caatctcaaa gcaatgtatg
    21301 agtcgttaga aaatgagaac ggcgatttaa aacagatcaa tgccaaactc atgctaagcg
    21361 atgtagcaat taaacaggaa aatactgcat tgaaagaaaa agcggatagt ttagcacaaa
    21421 tcaattcaaa aatgatgctt gcttcgttac aaaatagcaa agacatttca gaaattaaag
    21481 agcaactaaa tccagcttca aagggaggtg agtagtatgt ttagttttag cgatgtgaaa
    21541 atgatgtatg attggggctg tttcactaac gaacaagtaa tggttttcgt tccgttgtgc
    21601 attactgaag aa
//
LOCUS       DAFELO010000046        21503 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFELO010000046
VERSION     DAFELO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.046"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            5..403
                     /locus_tag="LP452_001870"
     CDS             5..403
                     /locus_tag="LP452_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL2014594.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="LP452_001871"
     CDS             387..1082
                     /locus_tag="LP452_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL2014595.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="LP452_001872"
     CDS             complement(1666..2553)
                     /locus_tag="LP452_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL2014596.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="LP452_001873"
     CDS             2738..3577
                     /locus_tag="LP452_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2014597.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="LP452_001874"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="LP452_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL2014598.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="LP452_001875"
     CDS             5045..5401
                     /locus_tag="LP452_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014599.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="LP452_001876"
     CDS             5404..6561
                     /locus_tag="LP452_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014600.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="LP452_001877"
     CDS             complement(6587..7027)
                     /locus_tag="LP452_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL2014601.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="LP452_001878"
     CDS             7187..7693
                     /locus_tag="LP452_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2014602.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="LP452_001879"
     CDS             7861..9774
                     /locus_tag="LP452_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL2014603.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="LP452_001880"
     CDS             9779..10780
                     /locus_tag="LP452_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2014604.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="LP452_001881"
     CDS             complement(10982..11188)
                     /locus_tag="LP452_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL2014605.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="LP452_001882"
     CDS             complement(11834..12682)
                     /locus_tag="LP452_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2014606.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12681..13748
                     /gene="gnd"
                     /locus_tag="LP452_001883"
     CDS             12681..13748
                     /gene="gnd"
                     /locus_tag="LP452_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL2014607.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGEHEIVEKGE"
     gene            13750..14751
                     /locus_tag="LP452_001884"
     CDS             13750..14751
                     /locus_tag="LP452_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL2014608.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="LP452_001885"
     CDS             14748..15032
                     /locus_tag="LP452_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL2014609.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="LP452_001886"
     CDS             15065..15862
                     /locus_tag="LP452_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2014610.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="LP452_001887"
     CDS             15876..16709
                     /locus_tag="LP452_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2014611.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="LP452_001888"
     CDS             16727..17584
                     /locus_tag="LP452_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2014612.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="LP452_001889"
     CDS             17606..18115
                     /locus_tag="LP452_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2014613.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="LP452_001890"
     CDS             18152..18553
                     /locus_tag="LP452_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014614.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="LP452_001891"
     CDS             18665..19018
                     /locus_tag="LP452_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014615.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="LP452_001892"
     CDS             19052..19495
                     /locus_tag="LP452_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2014616.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="LP452_001893"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="LP452_001893"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2014617.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="LP452_001894"
     CDS             20590..21303
                     /locus_tag="LP452_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2014618.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7401 a         3301 c         4490 g         6311 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcga acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttc
//
LOCUS       DAFELO010000047        21218 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFELO010000047
VERSION     DAFELO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.048"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            168..362
                     /locus_tag="LP452_001895"
     CDS             168..362
                     /locus_tag="LP452_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014619.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..929
                     /locus_tag="LP452_001896"
                     /pseudo
     CDS             631..929
                     /locus_tag="LP452_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1026..1460)
                     /locus_tag="LP452_001897"
     CDS             complement(1026..1460)
                     /locus_tag="LP452_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014620.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="LP452_001898"
     CDS             complement(1692..2402)
                     /locus_tag="LP452_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL2014621.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="LP452_001899"
     CDS             complement(2392..3252)
                     /locus_tag="LP452_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL2014622.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="LP452_001900"
     CDS             complement(3257..3895)
                     /locus_tag="LP452_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL2014623.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="LP452_001901"
     CDS             complement(3910..4209)
                     /locus_tag="LP452_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2014624.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="LP452_001902"
     CDS             complement(4252..5736)
                     /locus_tag="LP452_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL2014625.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLVIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="LP452_001903"
     CDS             complement(5756..6217)
                     /locus_tag="LP452_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2014626.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LP452_001904"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LP452_001904"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL2014627.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="LP452_001905"
     CDS             7520..8290
                     /locus_tag="LP452_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2014628.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="LP452_001906"
     CDS             complement(8563..9390)
                     /locus_tag="LP452_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2014629.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="LP452_001907"
     CDS             9958..11193
                     /locus_tag="LP452_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2014630.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="LP452_001908"
     CDS             complement(12192..12452)
                     /locus_tag="LP452_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL2014631.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="LP452_001909"
     CDS             complement(12479..13780)
                     /locus_tag="LP452_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2014632.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="LP452_001910"
     CDS             complement(14072..14530)
                     /locus_tag="LP452_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2014633.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15874)
                     /locus_tag="LP452_001911"
     CDS             complement(14543..15874)
                     /locus_tag="LP452_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL2014634.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17327)
                     /locus_tag="LP452_001912"
     CDS             complement(15885..17327)
                     /locus_tag="LP452_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2014635.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="LP452_001913"
     CDS             complement(17314..18078)
                     /locus_tag="LP452_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL2014636.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="LP452_001914"
     CDS             complement(18182..19057)
                     /locus_tag="LP452_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2014637.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="LP452_001915"
     tRNA            complement(19312..19396)
                     /locus_tag="LP452_001915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="LP452_001916"
     tRNA            complement(19414..19485)
                     /locus_tag="LP452_001916"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="LP452_001917"
     tRNA            complement(19495..19567)
                     /locus_tag="LP452_001917"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="LP452_001918"
     tRNA            complement(19629..19713)
                     /locus_tag="LP452_001918"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="LP452_001919"
     tRNA            complement(19734..19804)
                     /locus_tag="LP452_001919"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="LP452_001920"
     tRNA            complement(19850..19921)
                     /locus_tag="LP452_001920"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="LP452_001921"
     tRNA            complement(19933..20005)
                     /locus_tag="LP452_001921"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="LP452_001922"
     tRNA            complement(20008..20081)
                     /locus_tag="LP452_001922"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="LP452_001923"
     tRNA            complement(20088..20168)
                     /locus_tag="LP452_001923"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="LP452_001924"
     tRNA            complement(20170..20245)
                     /locus_tag="LP452_001924"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="LP452_001925"
     tRNA            complement(20281..20353)
                     /locus_tag="LP452_001925"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="LP452_001926"
     tRNA            complement(20363..20435)
                     /locus_tag="LP452_001926"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="LP452_001927"
     tRNA            complement(20451..20522)
                     /locus_tag="LP452_001927"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="LP452_001928"
     tRNA            complement(20537..20626)
                     /locus_tag="LP452_001928"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
     gene            complement(20666..20739)
                     /locus_tag="LP452_001929"
     tRNA            complement(20666..20739)
                     /locus_tag="LP452_001929"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20703..20705),aa:Asn,seq:gtt)
     gene            complement(20749..20864)
                     /gene="rrf"
                     /locus_tag="LP452_001930"
     rRNA            complement(20749..20864)
                     /gene="rrf"
                     /locus_tag="LP452_001930"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(20959..>21218)
                     /locus_tag="LP452_001931"
     rRNA            complement(20959..>21218)
                     /locus_tag="LP452_001931"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         6667 a         4472 c         3376 g         6703 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcactaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 aattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcgtcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gagaactccg gcagtaggac tcgaacctac gacatcatga
    20701 ttaacagtca tgcgctacta ccaactgagc tatgccggaa taacatcagc gtggcgacgt
    20761 cctactctca caaggggcaa cccctcacta caatcggcgc taagaagctt aacttctgtg
    20821 ttcggcatgg ttacaggtgt atccttctcg ctatcgccac cacactgtgg tgttatcttt
    20881 tattgagtaa attttgttca ctcaaaactg gatttgaagt cacataagtt tttttccgag
    20941 tacttttctt ttaacctatt ggttaagtcc tcgatcgatt agtatcagtc cgctccatac
    21001 atcactgtac ttccactcct gacctatcta cctgatcatc tctcagggat cttactttct
    21061 taaagaaatg ggaaatctca tcttgaggtg ggcttcacac ttagatgctt tcagcgttta
    21121 tcccttccct acatagctac ccagcaatgc ccttggcaga acaactggta caccagcggt
    21181 aagtccatcc cggtcctctc gtactaagga cagctcct
//
LOCUS       DAFELO010000048        20784 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFELO010000048
VERSION     DAFELO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.049"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(20..1576)
                     /gene="rny"
                     /locus_tag="LP452_001932"
     CDS             complement(20..1576)
                     /gene="rny"
                     /locus_tag="LP452_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL2014638.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1987..3036)
                     /gene="recA"
                     /locus_tag="LP452_001933"
     CDS             complement(1987..3036)
                     /gene="recA"
                     /locus_tag="LP452_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL2014639.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3145..4386)
                     /locus_tag="LP452_001934"
     CDS             complement(3145..4386)
                     /locus_tag="LP452_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL2014640.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4626..5204)
                     /gene="pgsA"
                     /locus_tag="LP452_001935"
     CDS             complement(4626..5204)
                     /gene="pgsA"
                     /locus_tag="LP452_001935"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL2014641.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5413..6336)
                     /locus_tag="LP452_001936"
     CDS             complement(5413..6336)
                     /locus_tag="LP452_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014642.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7075..8361)
                     /locus_tag="LP452_001937"
     CDS             complement(7075..8361)
                     /locus_tag="LP452_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL2014643.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8354..9622)
                     /locus_tag="LP452_001938"
     CDS             complement(8354..9622)
                     /locus_tag="LP452_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL2014644.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9874..10335
                     /gene="mscL"
                     /locus_tag="LP452_001939"
     CDS             9874..10335
                     /gene="mscL"
                     /locus_tag="LP452_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL2014645.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10394..10840)
                     /locus_tag="LP452_001940"
     CDS             complement(10394..10840)
                     /locus_tag="LP452_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014646.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10933..11178)
                     /locus_tag="LP452_001941"
     CDS             complement(10933..11178)
                     /locus_tag="LP452_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL2014647.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11292..11870)
                     /locus_tag="LP452_001942"
     CDS             complement(11292..11870)
                     /locus_tag="LP452_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2014648.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(11947..12477)
                     /locus_tag="LP452_001943"
     CDS             complement(11947..12477)
                     /locus_tag="LP452_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL2014649.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12481..13341)
                     /gene="rlmB"
                     /locus_tag="LP452_001944"
     CDS             complement(12481..13341)
                     /gene="rlmB"
                     /locus_tag="LP452_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL2014650.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13334..13729)
                     /locus_tag="LP452_001945"
     CDS             complement(13334..13729)
                     /locus_tag="LP452_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL2014651.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13726..15138)
                     /locus_tag="LP452_001946"
     CDS             complement(13726..15138)
                     /locus_tag="LP452_001946"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL2014652.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15135..15671)
                     /gene="cysE"
                     /locus_tag="LP452_001947"
     CDS             complement(15135..15671)
                     /gene="cysE"
                     /locus_tag="LP452_001947"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL2014653.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15757..15996)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15993..17453)
                     /locus_tag="LP452_001948"
     CDS             complement(15993..17453)
                     /locus_tag="LP452_001948"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL2014654.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17623..18780)
                     /locus_tag="LP452_001949"
     CDS             complement(17623..18780)
                     /locus_tag="LP452_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL2014655.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18814..20184)
                     /gene="radA"
                     /locus_tag="LP452_001950"
     CDS             complement(18814..20184)
                     /gene="radA"
                     /locus_tag="LP452_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL2014656.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20247..20774)
                     /locus_tag="LP452_001951"
     CDS             complement(20247..20774)
                     /locus_tag="LP452_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL2014657.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         5473 a         4593 c         3706 g         7012 t
ORIGIN      
        1 tcttcttttg tattttttat tatttagcat aatcagttgc acgagtttcc cgaataacga
       61 ttactttgat gtgtccagga taatccagtt cttcttcgat tcgtttacga atatcacgta
      121 ctaaacgaac agcgtctaaa tccgagattt cttctggctt gaccattacc cgaacttcgc
      181 gtccagcttg aactgcgtaa cttgattcta caccggcgaa gctgttcgaa atattttcca
      241 gacttcttaa acgacggatg tagttttcta atgattcact tcgcgcacca ggtcttgctg
      301 ctgagagcgc atctgctgca gctacaagga cagagataac agaagtagct tccacatctc
      361 catgatgaga agcgatcgcg ttgattacta ctgggttttc tttgtacttg gcagctaatt
      421 ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc aagtgctttc ccaatatcat
      481 ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc aagctctgct gctaagatac
      541 cagctaattt tgcaacctcg accgaatgct tcaagacgtt ttgtccataa ctagttctga
      601 atcgcagacg tccgatgatc ttgattagat ctggatgaag tgtatgagca ccgacttcaa
      661 acgctgcctc ttccccgtat tcgcgaatac gttcatccat ttctttacga gatttctcga
      721 ccatttcttc gatacgcgct ggatggattc gtccatcttg tatcagtttt tccaaggtca
      781 tccgtgcgat ttctcttcgg attggatcga atccggaaag aacaactgct tccggtgtat
      841 catcgatgat taaatcgata ccagtcagcg tctcaagcgt tcgaatatta cgaccttctc
      901 gtccaatgat acgacctttc atttcatcat tcggaagggt aacgactgaa acagtcgttt
      961 ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga taataagttt ttcgctttgc
     1021 gatctgcttc ttctttggca cgctgttcag attcttttac cattagagtt aactcatgat
     1081 tcagctcttc ttcagtcgat ttcatgatga catctcgtgc gtcctctttt gataaaccag
     1141 caattttttc tagttctttt tgttgatctt caatcatttt ttcaacgtct ttttctcgct
     1201 cttcaattaa ttgctgtttt gaactaagtg cttcttcttt actttcaagt gagttctcgc
     1261 gtttttctaa cgagtcatct ttacgattca gcgtttgttc gcgttgaatc aaacgattct
     1321 cttgagattt taattctaac ttactttctc tcagctcact ttcaacttca gaacggtatt
     1381 tctgattttc ttccttagct tctaacaatg cttctttttt tagagtttct gcttcttttt
     1441 ttgcactatc gatgattcca gcagctgatg attgtgcacc agctatttct ttctcgtgac
     1501 gtgactttgc gaccataaac cctaaaccaa gaccgacaat taaaccgatg atagcgagga
     1561 gaatgttgaa taccataaaa tccacctcca tactatcttt ttcattttta tcatctatac
     1621 agcttctatg ataaattatt ctaaactcgt caatatgcat cttcgcatat atgtgtacaa
     1681 aaaacatgca caactttatt ctaatgtttg taccccgtag tgtcaacttt aaataaacaa
     1741 agaaacggct ttctacttat tcattaaacg atggttttta aaataaatga cactagtttc
     1801 atataggtaa acctttaaat tgagaaactt ttctattaaa ttattctttg tagatgaaca
     1861 gatattttta ttttatgtac ccactcgtat aattaaataa ctcacgattt ctttattcaa
     1921 taaaaaaacg gcaggtcaag gagagtacct actcttcaga ccagccgttt ttgtttatca
     1981 taatttttat tcttctacta aaggcaattc ttcttgtcct ttttcatctt ctgggacagc
     2041 gacttcttca ccaataccat aagctgctct aactagagca gaaacttctg ccatcatttc
     2101 aggatgatta gccatataga ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt
     2161 ataagaatac caagcaccgc ttttatcaac aatgtctttt tctactgcca tgtctagaag
     2221 ttctccttct tgtgagatcc ctaaaccata catgacatct acttcagcga ttttgaaagg
     2281 aggtgctact ttgtttttta cgactttgat ctttgtacgg ttaccaacaa tatctgtacc
     2341 ttgtttcaat tgttctgcac gacgtacttc caaacggatc gttgcgtaga atttcaatgc
     2401 gcgtcctcca ggagtgattt ctggattccc aaacatcacg cctacttttt cacggatttg
     2461 gttgatgaaa atagcgattg tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc
     2521 ttgagacatc aaacgtgctt gtaacccgac atgtgagtca cccatttcgc cgtcgatttc
     2581 tgctcgtgga actaaagcag caactgaatc gactactacg atatctacag ccccacttga
     2641 tactaaagca tcagcgatct ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag
     2701 ttcatcgata ttcacaccta atttttgtgc atattgcgga tctaacgcat gctcagcatc
     2761 aatgaaagcg gccgttccgc catttttttg tacttctgca atagcgtgta gtgcaactgt
     2821 tgttttacct gaactttcag gtccatatac ttcgatgata cgtccacggg ggtaaccgcc
     2881 gacacctaaa gcgacatcta acgcaagaga gccacttgga attgtagaga tttgttggtc
     2941 gattttttcg cccaatttca taatagagcc tttaccatag ctcttttcaa tttttttcag
     3001 tgcagcatct aaggctgctt tacgatcatc tgccaatacg ataatcctcc ttatatttac
     3061 aaatcagagc aacatttcat tatccttatc atatcttttt tagtgaaaaa aagcaagaaa
     3121 aaaccgaaca aatattcgca tttatcattt tcttaaaagc tgttttctca aaagatcaaa
     3181 tccttgcata accgcattat atctgatagc agcacgatct ctggcaaagt gatatttttc
     3241 cgaaaccacg ccgtctttag atgaaatagc gatccatact gttcctgcag attgaccttc
     3301 taacgtgtcc ggtccagcca ctccggtgaa agctacactg taatctgttc ttgctatctc
     3361 acgtgcttga atggccatct gctccacaca ttctttgctg actgttccat attctgctaa
     3421 tagctccgga tcaatctcca gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc
     3481 gcctggaaat acttcagata caccagcaat gtcgcctaat gcggcttgga aggcgccggc
     3541 tgtcaaactt tccgctgctg ttacagtctg cttgttttct ttcagtagtt ccactacgac
     3601 ttttgctagc gaattatcat cgccgtatcc ataaaaatat tcccctactc gttcttggat
     3661 tttttcttct aatgctaaaa gagcttgatt ccctgcctga acatcatttg ttttcactgt
     3721 cagtcgtaat gtcacttcat ttggtttggc atatggtgcg atcgttggat tgatctgtgt
     3781 ctcaatcaga tcttttagct cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa
     3841 aactttagaa attagttttt cttcagaagg aaaacgttcc tctagtaaag gacgtacttg
     3901 ttccacaaac attggtttca actcattagg cggaccggga agaaggatat acgcatgtgt
     3961 atctgtttga taaaaagtcc ctaaagcaag ccctgtacga tttggtagag gaaccccgcc
     4021 ttcgataatc tgggactgtt gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa
     4081 atatgccaat aattttttgt atccttccgt attttgtatg agagacttac cgaggtgagc
     4141 cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct aatccgccac ataagagtat
     4201 gagttcactt cttgtttcag ctagagcaat caactcttct aaacgctgct gattatctcc
     4261 tacaactgtt tgataataaa cttcgatacc tagatcagct agctgttctg acaagaaagt
     4321 ggcattcgta ttcacgactt gtcctagtaa aagttctgtt ccgacagcta ttatttctgc
     4381 tttcattact tcccgctccc ttatatagta tacgctgtat ttttatattt ttttatttaa
     4441 aaagagggca aggagctgtg acaaaaatct tgtcacagct ccttgttcca aataaacagt
     4501 gttctcctgc caaaacctcg gtcataagtc acaatataca taaaacatga aaagctgttt
     4561 tttgaaatta tcccttgtga ctcagagtta gacggcaggc tcacaacctc tccactaaac
     4621 gatacttaca ttgatccttt gaacacaccg ctgtttttga tgaagtaatc cacacctgaa
     4681 tagatagtaa agatcagaca agcataaagc atgatttgat ctactggaat accgatcagg
     4741 ctgaatggga tattattgat aaacagcaag ataatagcaa ccatttgtgt agccgtcttg
     4801 acttttcccg gccaagctgc agccatgact tctccatgtt cgacaagtag taaacgcaag
     4861 cctgtgacgg ctaattcacg acagacaatg atcgctacta cccaaccagg ggcctttcct
     4921 tggtcaacca acatgataaa agcagtcata actagcattt tatctgccaa cgggtccgca
     4981 aacttgccaa aattagtgac aaggcctcgc gcacgggcaa tttttccatc taaccagtct
     5041 gtgatactag ctaaagcaaa aataactgca cctactagct gtgtgacagc cagtgtggtg
     5101 tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta caaccaaaat gaaaatcggg
     5161 atcatacaaa ttctaagtac agttaattta tttggtaaat tcacactatc gactcctttc
     5221 ttccctattc tacgtgacta tcagcgaagt ttccaccgat attatttact tttccaaatg
     5281 aatttattca aaggagtatt tttattgaaa ttgatctttt tgaattaacg aagaaggacc
     5341 gcgacacaga tttgtcacga tccttctttt ttatacctac aagtaaacag atgcgtaaac
     5401 taatttatac gatcattctg cgtattgaag agtaaggttc acattttttg gactctgatt
     5461 gttttcccct ggattaactg gtacggattc tccattcgct ttgatcgttg cattgcttgc
     5521 tgcaccaatc gtgatggtcg catttgttgc tgtttccggt aacacggtac tctgtgtttc
     5581 atttgctgcc agtgtccctt gataaacata ggcattgtct actagcacac ctaaccatac
     5641 acgattgaca gcagtaaatt caagggttac aggcttggta gcgtctgtga tatttactgc
     5701 gatggcactg ccagaatctt ctcctgtagc tattgccatt ttcttttctt cacttgattt
     5761 tgttgttgat tctactgtag aagactcttt ggtctgcgtg ctttgctctg aggaagagga
     5821 ctcctcgact gatccttcta ctttaagcga agaagctgtg tctccgatta ttggatcagc
     5881 ttgacgatct tgccaagtca tatagcccac gacaacgaca atcgccaaag caaccaatcc
     5941 taataaaatg acaggaagac ttgtccaaaa gcgacttgga tgcgattctt cctgatgcat
     6001 tgcttttcta gaagtactta ctgtttcagg ctgcggacgt tcagggaccg atcctgtcaa
     6061 tttgcttttc ccatcaaaaa catcgactaa atggtctcca tcttctccta cagcttcagc
     6121 gtattgccga ataaatgccc gcacgtaaaa tttaccaggc aattggtcaa aatcatcatt
     6181 gtcgattgct tccaaataac gtttttgaat ttttgtaatc tgctgtaatt catctaaaga
     6241 tattttttta ttcaggcgtg cttgccgtaa tttttctccg atggtttcaa aagccactcg
     6301 ttcatcttct ccagtctgtt tgattctccg aagcatctgc tgttgcaacg aaagacgtta
     6361 tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt tttccgaata aacattgttc
     6421 tcctgccaac tcctcggtca caagtcacaa tatccaataa atataagaag ctgttttttg
     6481 aacttgttcc ttgtgactcg gagttaaacg gcaggctcac gacctctttc ttaaacggtg
     6541 ttcaatcagc tgttcctcgg tattagctca aaaaccggca aaatataagg agatattttt
     6601 ccgtatgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg
     6661 tgttcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat taagaagctg
     6721 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttggtac
     6781 acggtgttcc agaagtagct tcttcggcaa taagccgaaa tatcacaaaa atttgagaag
     6841 caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctttg
     6901 attacttcgt ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta
     6961 ttttctcact aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg
     7021 ttcctactac aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt
     7081 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
     7141 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
     7201 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
     7261 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
     7321 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
     7381 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
     7441 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
     7501 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
     7561 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
     7621 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
     7681 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
     7741 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
     7801 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
     7861 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     7921 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     7981 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     8041 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     8101 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     8161 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     8221 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     8281 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     8341 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     8401 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     8461 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     8521 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     8581 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     8641 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     8701 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     8761 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     8821 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     8881 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     8941 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     9001 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     9061 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     9121 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     9181 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     9241 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     9301 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     9361 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     9421 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     9481 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     9541 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     9601 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     9661 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     9721 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     9781 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     9841 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     9901 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     9961 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
    10021 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
    10081 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
    10141 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
    10201 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
    10261 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
    10321 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
    10381 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
    10441 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
    10501 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
    10561 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
    10621 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
    10681 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
    10741 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
    10801 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
    10861 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg
    10921 ctcgtttgct ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac
    10981 atctgaatag ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac
    11041 gaatactgaa ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc
    11101 tgtctgagca accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga
    11161 agctaaagtt gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa
    11221 ttttcaagtt taccataaaa aaaaagaaat gcaataaaaa taaataagaa gctcattaag
    11281 atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt gagttttact
    11341 ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc catgtctaca
    11401 aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga aaactggatt
    11461 tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg ttcttgcaat
    11521 gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat tgagacttca
    11581 tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata gatttgattg
    11641 cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc gtaagttctc
    11701 aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac ataatttctt
    11761 aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata cagggcagca
    11821 aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat aaaataattc
    11881 ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac tgattagaac
    11941 gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt tttttaactg
    12001 ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt tattttcttg
    12061 ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag aagttcgtaa
    12121 tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg
    12181 tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag ccgtttcgtc
    12241 ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga tgcccggaac
    12301 tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat aatttgataa
    12361 acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa gctggacaag
    12421 ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt ttttcatggt
    12481 ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag caacactggc
    12541 atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca tttctttttt
    12601 caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg atcctttggc
    12661 attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa atcctgtttc
    12721 tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac
    12781 tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt gttttggtat
    12841 gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg
    12901 atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc
    12961 aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga ctccttgatg
    13021 cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga ctggcacagc
    13081 ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat cttcttgaag
    13141 aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt gcccaaagac
    13201 gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc gtccagtttg
    13261 ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg attgatgttt
    13321 gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag cggatcaatt
    13381 cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct tcgaaccctg
    13441 ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt ttagcatttc
    13501 ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc agcatttgct
    13561 gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga tgcagcttat
    13621 tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg taaatcgcat
    13681 ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg cttctcttct
    13741 ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca acaaatcacg
    13801 aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa
    13861 ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga agatcgaaag
    13921 ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt tcggctggtc
    13981 tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat ttgctgcatt
    14041 aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt
    14101 atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca aattgtcaaa
    14161 cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt cactgtagcg
    14221 gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt
    14281 tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt tttcatcgtc
    14341 ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt tccctgtttt
    14401 cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt gcccgccacc
    14461 atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt caatatgcca
    14521 accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt
    14581 ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg tacgctgact
    14641 tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat tttcaaattt
    14701 acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt caaccaacac
    14761 ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca gtgtagcagg
    14821 ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat cagcaacttc
    14881 tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta catccgtgaa
    14941 attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa
    15001 agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg gtccgcagac
    15061 atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc tggtcaatgt
    15121 attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg aacgactttt
    15181 gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac ggcacctgca
    15241 ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt cgcagagagc
    15301 atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt gccgcctaat
    15361 gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt
    15421 cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg gatctcaaca
    15481 ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag ccggtgctga
    15541 tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg
    15601 atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat cgctcgggta
    15661 aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat catcttctta
    15721 taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt ccgcgtggtt
    15781 ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat acgttcttgg
    15841 ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa gaccactccc
    15901 agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc tcttgtaatg
    15961 atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt taaatgtgct
    16021 aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc atgcatttgt
    16081 cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc agtttctttt
    16141 tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat ttgttctagc
    16201 tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac ttcttttgct
    16261 gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac gatttctgca
    16321 gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc gattgtcttt
    16381 tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca catgtcagta
    16441 aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag ttttttcgca
    16501 tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat caattcttgg
    16561 tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat aaagttgaac
    16621 attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa gattgattcg
    16681 tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat atgtccgaag
    16741 gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt attggcaata
    16801 tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca
    16861 aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc gatattatcg
    16921 gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt attgcgaggc
    16981 acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg ttcttcaggt
    17041 gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc
    17101 cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc
    17161 agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta agggccgtat
    17221 tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag gttttccaac
    17281 tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat acgaataata
    17341 aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct tgcatttcca
    17401 atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt cattttcttc
    17461 ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa
    17521 atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta aatagtaaag
    17581 ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg attgtttagt
    17641 ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa
    17701 gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga tacgttcatt
    17761 catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt aggctactcc
    17821 ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac cagggatgac
    17881 gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat tttggaattc
    17941 agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa gcttagccaa
    18001 tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt acatttcgac
    18061 agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac gaccacgcac
    18121 acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa caaaattagg
    18181 gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc gaccgtcaat
    18241 gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt ttcgttctaa
    18301 cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa
    18361 aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat agcctaagat
    18421 gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat tgtaaagagg
    18481 ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag
    18541 ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag ctgagatatg
    18601 tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa
    18661 tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg agggtaaaaa
    18721 tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac gtttttgcat
    18781 cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct tcaacgtttc
    18841 agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt tacctaagtt
    18901 gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga ctcgttgttc
    18961 aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga aacattccgt
    19021 tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag ctaaatcaat
    19081 cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt gattctgaag
    19141 aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat tgaaatcaag
    19201 acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg cttggatctc
    19261 taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag ttgcaccgtc
    19321 taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc catgctcatg
    19381 catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg ctcgcaaaat
    19441 ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca tatgttctaa
    19501 cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa aaatggctat
    19561 cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt ggctgacact
    19621 tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa tgataacata
    19681 atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt cagcgtatag
    19741 ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct gctctgcgct
    19801 ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct gagaaacttg
    19861 taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca tagaccctgg
    19921 caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga tccgaggttc
    19981 ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg tcaaacttgt
    20041 ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat tccattttcc
    20101 gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt ggcaaacaaa
    20161 ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca tgagacatta
    20221 aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc gccttgacgt
    20281 tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc ttggccgata
    20341 cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg gaacatgata
    20401 tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac tccgtttgtc
    20461 aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa aaattcatct
    20521 tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg tttttcattt
    20581 tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt ccagcctgct
    20641 ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc agcagcatga
    20701 tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat aacttcaaat
    20761 cctctttttc tcaccggttt tcct
//
LOCUS       DAFELO010000049        20285 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFELO010000049
VERSION     DAFELO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.050"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            140..460
                     /locus_tag="LP452_001952"
     CDS             140..460
                     /locus_tag="LP452_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014658.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="LP452_001953"
     CDS             912..1112
                     /locus_tag="LP452_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2014659.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="LP452_001954"
     CDS             1259..4048
                     /locus_tag="LP452_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL2014660.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="LP452_001955"
     CDS             4095..4622
                     /locus_tag="LP452_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL2014661.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="LP452_001956"
     CDS             4643..5833
                     /locus_tag="LP452_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2014662.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="LP452_001957"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="LP452_001957"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL2014663.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="LP452_001958"
     CDS             8224..8898
                     /locus_tag="LP452_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2014664.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="LP452_001959"
     CDS             8895..9236
                     /locus_tag="LP452_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014665.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="LP452_001960"
     CDS             9266..9625
                     /locus_tag="LP452_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL2014666.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="LP452_001961"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="LP452_001961"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL2014667.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="LP452_001962"
     CDS             12541..14007
                     /locus_tag="LP452_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2014668.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14622..15161
                     /locus_tag="LP452_001963"
     CDS             14622..15161
                     /locus_tag="LP452_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBL2014669.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15235..15675
                     /locus_tag="LP452_001964"
     CDS             15235..15675
                     /locus_tag="LP452_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL2014670.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15688..15897
                     /gene="copZ"
                     /locus_tag="LP452_001965"
     CDS             15688..15897
                     /gene="copZ"
                     /locus_tag="LP452_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL2014671.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15897..18083
                     /locus_tag="LP452_001966"
     CDS             15897..18083
                     /locus_tag="LP452_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL2014672.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18103..20274
                     /gene="copB"
                     /locus_tag="LP452_001967"
     CDS             18103..20274
                     /gene="copB"
                     /locus_tag="LP452_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL2014673.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6776 a         3527 c         4407 g         5575 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagtttcggc ttgtttttcg cagaagctac ttctggaaca ccgtgaatca gaggttgtga
    14161 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt
    14221 cgttttgctg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggta
    14281 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacaa cttctcatgt
    14341 ttgttgacta ttgtgccttg tgaccgaggt gttggcagga gaacaccgtt taaggaagag
    14401 gttgtaaaat cagcgatttg acctgagtat tagaggaaca aacgaaaaaa tagctcctca
    14461 tattttgccg ttttttgagt taatgctgaa ggtcagctga ttgaacacca tttattcgaa
    14521 acaagggact ttgacacgac ttttgtcaca gtcccttgtt tttttgcggc gttataagtg
    14581 gtatgatcga accaaattcc aaagagaagt gagggagaag aatgaagatc atcgtgattg
    14641 gacaaagtgg gtcgggaaaa tcgaccctcg caaggaagat caaagaaatc accgactttc
    14701 cgctactgcc gctagacctt ttatggcata caacagacta cagtattcag gcaaaaaagt
    14761 ggtttttgca agaacagcaa tcgtttatgg tatctaatcc gtcatggatt gttgaaggaa
    14821 attatacttc tacattagag gaaaggatta aagaagcaga taagatcatt tggcttaaag
    14881 tgccgcgtta tctcgccatc taccgagtag tttggcgttc tctaaaaaga aagataaaca
    14941 aaaaatctcg gccagatatg cctgaatcat tttcagagaa actaaacagg gaatacttag
    15001 aatttttatc ttttatctgg cattttgaag aaaacaatga accgaaaata cagcagctga
    15061 tccaagtagc taatgcaaga aataaactga tcatccttaa aacaagacga gagaaacaag
    15121 aatttttaac taaattgaaa atagagagca aaaataattg acagtttttg tttacaagcg
    15181 taatctgtat tcataagcta atgattacat ttgtaaacga aaggtgtgaa ataaatggaa
    15241 aaagtcaaaa cgccagtaaa gatcagtgat tcagagtggg agatcatgcg tgttatttgg
    15301 acacttggac agacgacagc ccaagaaatc acgcagatct tagctgattc gatggattgg
    15361 aaaccagcaa cgatcaaaac tttgctggga cgtttggtaa aaaaagaagt gatctggaca
    15421 gaacaagaag gaaaaaaatt catttatcat ccagctgtct cggagatgga aacagttcgc
    15481 agtgcgacgg aaaatctttt ttcccatatc tgtgcaaaaa ggattggaga gacgattgcg
    15541 gatctagtag aagaagccac tttgactcaa gaagatatca atgagatttt gaaacaactg
    15601 gaacaaaagc aaccagtaga tacgatcgaa tgtaactgta tcccaggtca atgtgaatgt
    15661 aaacaacatc aataggagga agaaaaaatg aaacaacaat tttcaatcga aggtatgagc
    15721 tgcaatcatt gtgcagcaag agtagaagaa acagtaagcg cacttgacgg cgtgcaaaaa
    15781 gtgaaagtaa atttgaaaaa agcaaacggt acggtcaagt ttgatgaaac aaaagttcaa
    15841 tccgaaaaga tttgtcaagc aatcaatgga ttgggttata aagcagaggt gatttgatgg
    15901 caacagcaga aaccaaaatg gatacgttcg tcattacagg tatgacttgt gcaaattgtt
    15961 ctgctaggat cgaaaaagag ctgaacggac aacctggtgt ggtcaatgcc acagtcaact
    16021 tggccactga aaaagcaagt gtcaaatatg aagggacgac cactgaaaaa ttaatccaaa
    16081 gcgtagaaaa catcggttat ggtgcaattt tgtacgatga agcccacaaa caaaaaattg
    16141 cagaagaaaa gcaagcgtat ttgaaaaaaa tgctattcga tcttatattg agtacggttt
    16201 tgactttgcc gttgatgttg tcaatgattg ctatgatgtt aggaagccat gcggcgattg
    16261 ttcacttttt tcattttcca atcgtccagt tagtgctgtc tgctcctgtc caattttatg
    16321 tcggtgctcg tttttataaa ggtgcgtacc atgcaatcaa gacgaaagca ccaaatatgg
    16381 atgtattggt agcaattgga acatcggctg cctttgcatt gagcatttac aacggatttt
    16441 tccgtggaca cccccaagat ctttattttg aaagcagcag tatgatcatc acgctgattt
    16501 tgctcggcaa atatttggaa catacagcaa aaacgaaaac aggtaatgca atcaaacaat
    16561 tgatgtctct tcaaacaaaa accgcccaag tgataagaaa cgggaaagaa gaaacgttag
    16621 cgattgaaga agtagtcgtt ggtgatcaac tagtcatccg tcctggtgag cagattccag
    16681 cagatggtcg aattatttca ggaagcagtg cgatagatga aagtatgctt actggtgaaa
    16741 atcttcctgt agaaaagaat ccggatgaca cgctttttgg cgggacaatc aatacgaatg
    16801 gtctgctaca catggaagtc acacaagtcg gcaagcagac agtattagcg caaatcattc
    16861 aaatggtgga agatgctcaa gggagcaaag ccccaatcca aaaaatcgca gatcggatct
    16921 cagggatttt tgttccgatc gtattagtca tcgcgtttat cacgctcata gctacaggtt
    16981 tgatcactgg tgattggcaa ctcgcattga tccatagtgt ctctgtatta gtcattgcgt
    17041 gtccatgtgc attaggttta gcgaccccaa cagctattat ggtaggaacg ggtgttggtg
    17101 cacgaaatgg aattttaatt aaaggcggag aagcattaga agcagctgct cacttagaca
    17161 gcattgtttt ggataaaact ggaacgatta cagaaggaaa gcccaaagtt acagatctag
    17221 tcggttcaaa agaagtccta tctatttttt atactttaga acaagcttct gaacatccac
    17281 taggtaaagc aatcgtggaa tacggaaaac tgcaagaagc agcaacatac gatatgattg
    17341 attttacagc ccacccaggt gctgggatca gcggtacgat taatggagta cgctattttg
    17401 cgggtacacg taaacgatta attgaattga acctatcatt tgatgaatac caagaacacg
    17461 cgttagaatt agaacagcag ggaaaaacag tcatgttttt agctgatgaa aagcaagtga
    17521 tcgggtggat tgccgtggct gatcaaatca aactagaagc caaacaagca atcaagcagc
    17581 tccagaataa aggattagat gtttttatgc tgacaggaga caacaaactg gcagccgaaa
    17641 cgattggtaa acaagttggt attgatccaa agcatatctt tgctgaagta cttccagagg
    17701 ataaagcagc ctacgtagaa aaacttcaga aagatggcaa aaaagtcggt atggcaggcg
    17761 atggaatcaa tgatgcaccg gccttagcat tagctgatgt cggtatggca atgggaagtg
    17821 gaacggacat tgcgatggaa acagccgatg tgacgctgat gaatagcagt ttggcatcca
    17881 ttgcacaaac aatcgagttg tctaggttga cattgagaaa aatcaaacaa aatcttttct
    17941 gggcttttgt ctataatacg attggtattc cttttgccgc actaggcttt ttgaatccaa
    18001 tcattgcagg aggagcgatg gcgtttagtt ccgtcagtgt attgttgaac tccctgagct
    18061 taaatcgaca catgaaaaag taagagaagg gttggagtaa aaatgaatgg gaacgatatg
    18121 gagaaaaaac atgagcataa aaaagaagta gtaaacaaag aaagtacaga atctcatgaa
    18181 aaacagatgg agatgaaaca tgaccatagt cagatggatc attccatgca catgggacat
    18241 aaccatggag atatagcaaa cagcaaacaa atggaccatt cgatgcacat ggatcacgaa
    18301 cacggcggca tggatcattc catgcacatg gggaatttca aacaaaaatt ttggctatcg
    18361 cttatcctag ctatcccaat cattcttttt tcaccaatga tgggaatgga atttccattt
    18421 caagtaacgt ttcctggttc tgattggctc gtattgatct tagcaactat actatttatc
    18481 tatggcggtc agccattttt aagtggagct aagatggaat tgaaacaaaa aagtcctgct
    18541 atgatgacac tgatcgcgat ggggattaca gttgcatata tttatagtgt ctattcgttt
    18601 atcgcaaatc tgctcaatcc acatacacac gtcatggatt tcttctggga attggctact
    18661 ttgatcgtta ttatgctact aggtcactgg attgaaatga atgctgtgtc gaatgccagc
    18721 aatgctttac aaaaactagc agaattactg ccagaatcag tgaaacgctt gacgaaagat
    18781 ggaaaagaag agacagtttc attgaaagaa gtaaacgaag gtgatcgttt gatcgtccgg
    18841 tctggtgata aaatgccaac agatggtgta atcttgaaag gtgaaacaat tgttgacgaa
    18901 tcagctgtga ctggcgaatc aagaggaatc aaaaaacaag ccaatgacaa agtgattggc
    18961 ggttctatca atggggatgg aacgatcgaa atcgaagtaa ctggaactgg tgaaaatggt
    19021 tatctagcga aggttatgga aatggtgcga aaagcacaag gagaaaaatc caagctagag
    19081 tcattatctg acaaagtggc aaaatggctg ttttatgtcg cattaattgt gggtatcctg
    19141 gcattcatcg cctggttgtt cttgacagat cttcctaatg cattagaacg tatggtaaca
    19201 gtctttatca tcgcctgtcc tcatgctcta ggtttagcta tcccattagt tgttgctcgc
    19261 tccacatcaa tagcagctaa aaacggatta cttttgaaaa accgcaatgc actggagcaa
    19321 gccaatgatc tagatgtgat catgctggac aaaacaggaa cactaacaga aggaaaattt
    19381 actgtaacgg gtgtcgaagt acttgatgat gcgttcaata aaaatgaaat ccttcaatat
    19441 ctaggagcct tggaagcgaa tgccaatcat ccattggctg ttggcattat gaattatttg
    19501 aaagaacatg aaatcaagcc ttatcaagct gaaaatctaa aaaatctttc aggagtcggg
    19561 ctagaggcaa cagtcaaaaa acaacaagtg aaaatagtta atgaaaaaga agttgagcgt
    19621 ctcggattaa acgtagaaca agcattattg aaaccttacc aagaacaagg aaataccatc
    19681 agcttcctga ttttagaaaa tcatctagct gcaatcgttg ctttaggtga cgtagtaaaa
    19741 acagaagcaa aagaatttat ccgaacattg aaagaaagaa aaatcactcc tgtcatgctt
    19801 actggtgaca ataaaaacgc tgcccaagca gtagcagatt atttagggat cgaagaatat
    19861 tatggcggtt tattaccaga tgataaagaa gctgttgtcc agaaatacct tgaccaaggg
    19921 aaaaaagtaa tcatggtggg tgatgggatc aatgatgcgc caagtttagc ccgtgcttca
    19981 atcgggatgg caatcggtgc agggacagat atcgcaatcg attctgcgga tgtcgtattg
    20041 acaaacagcg atccaaaaga tattttgcat tttcttgatc tagccaagca aacgcgaaag
    20101 aaaatgatcc agaacctctg gtggggggca ggttacaata ttcttgcaat tccgctagca
    20161 gccggaattc ttgcgccaat tggtatcata ttaagtccag cagttggtgc tgtcttgatg
    20221 tcgttaagta cggtagttgt agctctaaat gctttgacat tgaaaatcaa ataataatga
    20281 aaaaa
//
LOCUS       DAFELO010000050        19490 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFELO010000050
VERSION     DAFELO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.051"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(519..1040)
                     /locus_tag="LP452_001968"
     CDS             complement(519..1040)
                     /locus_tag="LP452_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL2014674.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            1239..4931
                     /gene="nifJ"
                     /locus_tag="LP452_001969"
     CDS             1239..4931
                     /gene="nifJ"
                     /locus_tag="LP452_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL2014675.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            5120..6007
                     /locus_tag="LP452_001970"
     CDS             5120..6007
                     /locus_tag="LP452_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL2014676.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            6004..7173
                     /locus_tag="LP452_001971"
     CDS             6004..7173
                     /locus_tag="LP452_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014677.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            7197..8552
                     /locus_tag="LP452_001972"
     CDS             7197..8552
                     /locus_tag="LP452_001972"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL2014678.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8624..10429)
                     /gene="glmS"
                     /locus_tag="LP452_001973"
     CDS             complement(8624..10429)
                     /gene="glmS"
                     /locus_tag="LP452_001973"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL2014679.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10838..11128
                     /locus_tag="LP452_001974"
     CDS             10838..11128
                     /locus_tag="LP452_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014680.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            11141..12166
                     /gene="sppA"
                     /locus_tag="LP452_001975"
     CDS             11141..12166
                     /gene="sppA"
                     /locus_tag="LP452_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL2014681.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            12169..12828
                     /locus_tag="LP452_001976"
     CDS             12169..12828
                     /locus_tag="LP452_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2014682.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12985..13917
                     /locus_tag="LP452_001977"
     CDS             12985..13917
                     /locus_tag="LP452_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014683.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            14071..15360
                     /locus_tag="LP452_001978"
     CDS             14071..15360
                     /locus_tag="LP452_001978"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL2014684.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15607..16857
                     /gene="clpX"
                     /locus_tag="LP452_001979"
     CDS             15607..16857
                     /gene="clpX"
                     /locus_tag="LP452_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL2014685.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16940..17527
                     /locus_tag="LP452_001980"
     CDS             16940..17527
                     /locus_tag="LP452_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL2014686.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17601..17756)
                     /locus_tag="LP452_001981"
     CDS             complement(17601..17756)
                     /locus_tag="LP452_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014687.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17892..19220
                     /locus_tag="LP452_001982"
     CDS             17892..19220
                     /locus_tag="LP452_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014688.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            19231..19440
                     /locus_tag="LP452_001983"
     CDS             19231..19440
                     /locus_tag="LP452_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014689.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6398 a         3450 c         4191 g         5451 t
ORIGIN      
        1 aatacagatg cgtatgcatt tgtgtttgcc atcttgcgtt tggtagacga gataggtata
       61 agctaaagaa acaacttgtt taaaataaac gacctagctc aacaaatgac catctaatca
      121 actaacagca tacataaaag caactatttt tgaagtagaa ttgaaatcgg gataacttaa
      181 aaagtgattg ggatactagt gagaatcacc gtacataaat attactcgtt aagggaaatt
      241 gagaaatttt gtatgaaaat ttaagaatta gttagtatgc gcaagtcaaa aatatgaata
      301 aattatttgg tggagataat ttgcttagct tgtcataaga aatgagataa tctggcagat
      361 gcaagctttt ggacaccatt tattcggaat aaggaggctg ggacagaagc gtttaactcc
      421 aagaaataag aaggagttgc ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca
      481 aaagtgttct ttacttttgt cccacgctcc ttattttttt attttgtttg gatcaattct
      541 tttaattcct ctattgaaat agattcatca aagatttctt tacccaagat gacagttgga
      601 acaaatcgaa tatgtgcttg ctcagcttcg ttgatgacag cttggctcag ctgttcgtct
      661 ttttgttctg tcagctttag tttatctact gcaaaatccg ctacttcttg gagagaaaga
      721 tgtttccact cgtcttgagt atcgaatatt tgtttgatct ctttgatcgc tttttggcca
      781 tcgctgattg tgagataccg gtgcatcacg ttccctcgct gtaagctttc tttttcttta
      841 tcgaacaatt tgatcactcg ctgcaattgc ccgctttgga ctgcttcttc taatagctca
      901 taagattctt caaaccattg tcggcaatac ggacaagcta agttgatgaa ttcgaccatt
      961 tttttaggag cattcgatga accataaagg attccttttt gtgtatttgt tttcgttgca
     1021 tcaatcactg atatatccat gttaaaacac ctctttcgct tatagtttat acatgtctga
     1081 caaaaaaagg caacgattta gactgtttgt ctaaaaatta gatgaccaat tcaaatagat
     1141 acatgtaaaa tatttttata aataaaagaa aaaaatggca tgttttacgt tataatggaa
     1201 cagataataa gagagaatat gaacaaggat gatgaatcat gcgaaaaatg aaaacaatgg
     1261 atggcaatac ggcagccgca tatatttctt atgcatttac cgagattgct gcaatatatc
     1321 caataacacc cagttcgacc atggctgaat tagtagatca atggtcagca gcaggaaaag
     1381 aaaacgtatt cggtcaacca gtaaaagtag tagaaatgca atccgaagca ggagcagctg
     1441 gtgtggtcca tggatcattg aaaactggtg cactgacaac tacatatacc gcttcacaag
     1501 ggctgctttt gatgataccg aatatgtaca agattgctgg ggaactactg ccaagtgttt
     1561 tccacgttgc tagtcgggca gtgacgacaa atgcgttgaa tattttcgga gatcacggag
     1621 acgtgatggc tgcaagacaa acaggatttg ctatgttagc tgaaagcagt gtacaagaag
     1681 tgatggattt agcacctgtt gcccatcttg cttctattga aggagaagtc ccttttttga
     1741 atttctttga tggattccgt acgagtcacg agattcaaaa aatcgaagtt ttggaatatg
     1801 aagaattagc tcctctgatc aatcaggaga aattagcttc atttcgtaaa cgagggatga
     1861 atcccaatca tccgactgtt agtggtacga accagaatcc agacatccat tttcaacaac
     1921 gggaaacgat caacccctat tatgataaat tacctgaaat cgttcaaaaa tatatgaaag
     1981 agatcaaccg attacgagga acttcttatg acttggtgac ttattacgga gcagacgatg
     2041 cggaagaagt cattgtttct atgggatcag ttgcacagac gattgaacaa acgattgatt
     2101 acttgaatgc aaacggacgg aaaacaggtt ttttgaatat ccatctttat cgtccttttc
     2161 cagtggaaac attcgtgcaa aagttgcctc atacagtaaa aacaattgct gttcttgatc
     2221 gaagtaaaga accaggggca ggtggagaac ctctcttatt agacgttcaa agtgcgttat
     2281 atgattctga acttcgcccg gctgttattg gtggtcgcta cggattgggg tctaaagatg
     2341 tgactcccga ccaaatctca gcagtttttg atgaattgaa aaaagaccca agcatacgca
     2401 aaaaacggtt taccataggg atcgtggatg atgtgactta tcaatctttg gaaccaaagg
     2461 aaagtctgga tttgacagaa ccgcaaacat tccaggccaa gttttgggga tttggatctg
     2521 acggaacagt tggagcaaat aaatcagcta tcaagattat tggagatcac accgataaat
     2581 atgcgcaagg ctatttttat tatgattcga aaaaatctgg cggtctgact gtctctcatc
     2641 ttcgttttgg agatacgccg atacgttcag cgtatcttgt cgagcatgcg gaccttgttg
     2701 cctgtcacac accagcttac ctgcatagct atgacttggt caaaggattg aagccaggtg
     2761 gcattttttt attgaatact ctttggagcg acgaacaatt agaaacgcat ctgcccttaa
     2821 aattaaaacg ttacttggct gaaaataaca ttagattcta tacgatcaat gccatgagat
     2881 tggcccaaga agtcggtttg ggacgccgga tcaatacggc aatggaaacg gcttttttta
     2941 aattagctga catcattcct tttgatgagg tgcttccgct gctaaaagaa gaagccctga
     3001 aaagctatgg acataaatca atggctatcg tagaaaaaaa catacaagca atcgatcgga
     3061 cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg gagaacgttg gagatgcctg
     3121 aaaaaatcaa taaaaggaac actggttata tacaagaaat acttgaacca gtgaatgcac
     3181 aggaaggaaa tcaactctct gttggtacac ttgtagcaaa tggaatgaca actggcgaaa
     3241 tgcctttagg aacggcttct atggaaaagc ggggcgtcgc tttagaagta cctgagtgga
     3301 ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt ttgtcctcat gctgccatcc
     3361 gtccattttt agcagatgaa gaggaaatgg aagaagcgcc agaaggattt atcgtgagag
     3421 aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt ttcagtggaa gactgtacag
     3481 gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa agctttagtg atgaagcctt
     3541 atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc aatgacatta agacaaaaag
     3601 caaatccagt caaaggaaag aatactgtct tgtctacaca attcaatcaa ccgcttcttg
     3661 aattctcggg cgcttgctca ggctgtggcg aaacacctta tgtaaaatta ttgacacaaa
     3721 tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg ttcttctatt tggggcggtg
     3781 cttctcctgc ttctccttat acgaccaatg aatgtggtca agggcctgca tggtcaaatt
     3841 ctttattgga agataatgct gaatttggtt atggtatgct tctagcggca cagactcgtc
     3901 gcgaggcttt ggcaagttca atcaaagaag tattgccaaa agtatcagaa gaactacgag
     3961 aattgatgga agactggtta gtacacatgt ttgagagtga aggaacgcag caaagagcag
     4021 caaaactgca agctgcgatc gaaagcgaac gatcaatttt ccctgaactg gaggatatat
     4081 acaagagaaa ggatctattc gtaaaaaaca gccaatggat gattggtgga gacggatggg
     4141 cttacgacat tggttttggc gggatcgatc atgtacttgc cagcggagca gacgtgaata
     4201 tccttgtttt ggataacgaa gtctattcca atactggcgg tcaaacctca aaagcaacac
     4261 cagcttcggc aatcgctaaa ttttcggcta gtggaaagta tgcttcaaag aaagatctag
     4321 gaatgatggc gatgacatat ggaaatgttt atgtggcgca aattgcttca ggtgccaatc
     4381 aaatgcaaac gatcaaggca tttgaagaag cagaaaagta tcctggtcct tctctgatta
     4441 ttgcctatac gccttgtatc acacatggct tgcttggcgg aatgaaagaa tcgcttaaag
     4501 aagcaaaaga agctgtttct tctggttatt ggtcattata tcgatataac cctacaatgg
     4561 tcgaaaaaaa caagaaccca atgactttgg actacaaaaa acctgatttc tctgctatgc
     4621 ctgatttcat gaggaaacaa gttcgctttt cctcgctaga taacgcaaat ccaaatcttg
     4681 caaaagcttt atttgaaaag actgtccacg acgctaaaac acgtttttac aactatgctg
     4741 cactgacggg agaattagaa aaaatcaaga aacgacttga accagaggaa tctatcgatg
     4801 tacccgttaa gactagaaaa gaaaaaacga cggaccttga agcagaagaa agaagagctg
     4861 ctagaagagc tgccagagca gaacgacgta aaaaaatgga acaagctaca aaagaaaaaa
     4921 aggaaaaata acagaaaaaa tataatgaat aattctctga agtttaaaaa tatttattgc
     4981 agagaaaaat aaaacgcata cttgcaattt tcttgaaaat tatgcgtttt tctcaagaaa
     5041 aatacgagca tatcagcctc taaaattaaa acatatgata tactagagag gaaatctcaa
     5101 aagagaagag aggcttttta tgtcatttca aatcggggaa ctttttaatt tggactattg
     5161 gcagcaaatg ttttccggtg acttgttctc tctgaatttt ttggtgaaca tacttgatat
     5221 tctggttgtc tggtatcttg tatacaaact gatccaactg ctgcgaggca caaaagcgat
     5281 tcagcttctg aaaggtgtag ctatttttat tgttatccga atcgtagcgg aaatcattgg
     5341 tctgcacacc ttgtcttggc tgatgaacca agtcattacg tacggggtga tagctgctat
     5401 cgtgattttt cagccggaaa tccggagagg actggaacat cttggacgca gttcgctttt
     5461 caaacaatca aaaagcgaac agcaggaaga tgagaagatg atcttgtctt ttgacaaagc
     5521 catccaatat atgtccaagc gaaaaatcgg tgcgttgatc actatcgaac gtcatactgg
     5581 attagatgag tatattgaga cagggattgc tttagatgca gatatcactg gggagctgct
     5641 gatcaatatc tttattccta atacaccgct gcatgacggt gcagttattg taaaagaagg
     5701 aaagatcgca gtagcaagcg cctatcttcc tctatcagaa agtatgttga tccctaaaga
     5761 attcgggact cgacaccggg cagctgtcgg tataagcgaa gtcagtgacg cgattaccat
     5821 cgttgtctct gaagaaaccg gggacgtcag catcacgtta gataatgaac tgatggctgg
     5881 cttatcccag caagaatatt tggcaatctt gcgtagagaa ctaattgctg aagagaaaac
     5941 aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt atgactaagg gaggggatcg
     6001 caaatgatct caaaagagaa acggacaagc attctttaca gttttattgc attgctgttc
     6061 agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc caaatacgct ttctggaaat
     6121 gaagcgtata atcaaacagt tacgggggtg ccgatcaaga tttcttatga ttcaaagcgc
     6181 tactatatcc atggatatga aaatgcagtg actgtcaaac tttccagtgc aaaccgagtt
     6241 cagttgaatg cagaggcaaa cgaagataca cggatgttcc gcgtcacagc agatatcact
     6301 aagctgggcg aaggaacaca tgaagttccg ctaaaaattc aaaatttgag cagtgctgtc
     6361 acagcaaaga tcgaaccatc gactattaca gtaacggttg agaaaaaggt aataaaagat
     6421 ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat ctggatatga acttgatgat
     6481 acttccgtta gtcctaaaaa agtctctatc actacaggtg acaaaacatt ggcagaaatc
     6541 aaacgagtag tagctgatgt ggacgcttcg atggtcacag atgatgggat taatagtgag
     6601 gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa gtatcgtctc tgatccagtg
     6661 caagtaacag tgagagctga ttcgatcaag ccaagcaagt cggtacgact ttatgggata
     6721 cagcaaggaa caccagctgc cggggtagaa agctatgatt tcagtttttc aaaactggaa
     6781 gcagaagtaa gcggctctag cgatttgctt gcatcgatcg gtgacagtat tgctgtaccg
     6841 atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa ttgaaatacc agtagaagag
     6901 gggttaagta tcaagcccga atcagttagt gtggagatta cacccgtgtt gcaaacgtca
     6961 agcagtagct ctgcttcgac gagcagtcgg acacaagaaa atacaagtac gagtagaagt
     7021 atttcatctg ctgattcagg aacggaacca tcgacaacaa ccatttcttc cagttcggaa
     7081 tctgggacaa gcagctcgac cacaagtaca gaagaaacat cagaaacaag cgaatcaaca
     7141 caaatgagca gaacaaattc ctcagaaaat taagttaaag gagtaatttt acaactatgg
     7201 gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa caaggaactt acgccagaac
     7261 ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag ccagcatgaa gaagaaggaa
     7321 aacgcccacg cgtgttagta gggcgggata caagaatctc aggtcaaatg ttagaagaag
     7381 cgctgatttc aggactttta tcggtgggta tcgaagtgtt ccagctaggt gtgatctcta
     7441 caccaggtgt tgcttatctg acacgtttgc aaaaagcaag cgcaggggtc atgatttctg
     7501 cttctcataa tccagcacaa gataatggaa tcaaattttt cggtgcagat ggttttaaac
     7561 tagtagatga ccaagagcta gaaattgaag cattattaga tgctgaagtg gatgaattgc
     7621 ctcgtccatc agctgaagga ttgggaactg tagaagaatt tccagaaggc ttattgaaat
     7681 attctcaatt ccttgtacaa acgatcaacg gtgacctttc cggtttgacc atttgtgtcg
     7741 atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt gtttgccgat cttgaaacag
     7801 atttttatac aatgggcaca aatccagacg ggttaaatat caatgacggc gtaggctcta
     7861 cccatccaga acgtttagct gagatggtcg ttgaaaaagg agcagatgct ggactagctt
     7921 tcgatggcga tggcgatcgt attattgcgg tggatgaact tggaaacatt gtcgacggtg
     7981 ataaaatcat gtttatctgt gctaaatact tggcagaaaa aaatcgattg aaaaaagata
     8041 cgatcgttac gactgtcatg agtaacttag gattccacaa agcggtggaa ggaatcggtt
     8101 tgaaagatgt cgttacacaa gttggtgatc gttacgttgt agaagaaatg cgcaaaaacg
     8161 attataactt tggcggcgag cagtctgggc acatgatttt cttagatttt aacacaactg
     8221 gagatgggat gctttcagga attcagttac tgaatatcat gaaacagaca ggtaagaaat
     8281 tatcagagct tgcagcagag gtaacaatct atccgcaaaa attagtgaat atccgcgtaa
     8341 gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa agtgattgaa gaggcagaag
     8401 ctgaaatggg atcagaagga agaatcctag ttcgtccttc cggtacagaa ccattacttc
     8461 gagtgatggc agaagcgcct actgatgaga aagttaatta ctatgtcgat aaaatcgcgg
     8521 cagtagtcaa agaagaaatc ggactggatt aagagatatt ttcataaaaa ataaaagaac
     8581 aaggaccagt gtcagatgtc tgacactggt ccttgttctt ttattattct actgtcactg
     8641 atttcgctaa attacgtggt ttgtcaacgt cgaatccccg taatagcgtt gcgtaatatg
     8701 caagtaattg agtcggtacg actgtggtta gagtagttag ataagggtgg acagttggta
     8761 gcaccagctg gtcactttcc ttttctaatc cctctgagac aataactagt gttttagcgc
     8821 cacggctttc gacttctttc aggtttcctc gagtatgcgc accagtcact tcttcactga
     8881 tgattccgat cactggtgtt ccttcttcga tcaaagcaat cgttccatgt ttcaattcgc
     8941 cagcggcaaa accttctgcc tggatataac tgatttcttt caatttcaaa gcagcttcaa
     9001 gagaaacgtt gtagtcagca ccacgtccaa tgtaaaaggc atttcttgta tcgctgagat
     9061 atacttctgc caactcttct aaataatctt tttgggcgat gaccgattcc atcgcattag
     9121 caactaaact caattcatga gtgatatcga atacaagaga agtttcaaat tcttttttat
     9181 caccgatagc tttggctaat aaagttagta cagctatttg tgcagtataa gctttcgttg
     9241 atgctactgc gatttcgggt ccagcatgca atagcagtgt aaaggatgct tcccgcgaaa
     9301 gagtagaacc agcaacattt gtaatcgtaa gtgaagggta gccgagacgg ttgattttta
     9361 ccagcacttg gcggctgtct gctgtttcgc cactttgact caagaaaata aagaatggtt
     9421 tttttgaaag aagaggtgtg ttatagccaa attcactggc taaatgaacc tcaacaggaa
     9481 tctgtgtcaa tttttccaaa gtctgtttac ctgctaaccc cgcatggtaa ctagttccgc
     9541 aagcaacgat atagattcga tcgctatcag acaatgtatc gaccagcttt tgatcaagag
     9601 agacacgatt gtcttctgtt acgtattttt gagcgatacg acgcatgata ataggctgtt
     9661 cgtcgatttc tttcagcata taaaaaggat aggtaccttt ctcgatatct gaagcatcta
     9721 cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc aaaattttga ataataactt
     9781 tttctgatgt gacggtaacc atctcttcat cttcaatctc aacgaattcc ttcgtatgag
     9841 caagcatagc cattgcatcg ctggcaatca cattgaatcc atcgccgagt ccgattaata
     9901 gtggactttt atttttagcg acgtaaataa catcaggtgc tgtccgatca atcaaagcga
     9961 aagcgtaaga acctttgatg acccgcaaag cttttaaaaa cgcctcttgc gttgtagtat
    10021 ccttcgcgaa agtttcaatt agatgagcga taatctcagt atcggtctca ccaataaagt
    10081 ggtcatttgc taaataagca attttaagtt cttcaaagtt ttcgattacg ccgttgtgaa
    10141 cgagcacgaa gcgtccactt tgggaagtat gtggatgggc gttttcttca gaaggttgcc
    10201 cgtgagttgc ccatcgtgta tgaccaatac ctatatttcc tgtagtatca atatttattt
    10261 tgtttttcaa attttgaatt ctgccttgtg ctttgaccag ataatcagtt gtgccatctg
    10321 agacaaagat accagctgaa tcataaccac ggtattctaa tttttctaga ccgtcaatca
    10381 atcctggtgt tacatttttt aatccaatca ttccaacaat tccgcacata aaaaacatcc
    10441 tactttctat aatgaattcg tatgaatgct tccagctgca ggaaaatcct tttgttttat
    10501 ctttgcagac attttttgtg gattttctgt agagtgcagt ccctagggcc atccgccgaa
    10561 tctttcgata aaccctctcc tcgtcatctg tttacacaga cctggcgcta tcctaaaagg
    10621 tataactatc ctcctttttg ttttttatct ggaaacattt aaaatcataa ccttttgcga
    10681 atgttcttgt caacaaaaaa agaataaaaa ataaattggt atatatcaaa ttctttgtca
    10741 aatgtccttt ttatgtcaaa aaataatggg tttttatcta tcatcttttt caggagttcg
    10801 gtacaataga aagtaatcgg atagaggagg agtaaaaatg gattcaaaaa ttgagatcat
    10861 gacactggga atgttaaaaa aacaattgtc caaattcgaa gcatcagctg gtgtatcaga
    10921 tgatacgaaa atatttttag atactggctg ggatagcatc caagaaatcg cacctgatgc
    10981 cttggaagtt gctcaagctc gtgagtttac ggtagaagat gaattaacaa aagagtcgtt
    11041 ttccgggtat gcgcgggaag aaaaagcaga gcggttcgac acttctgagc agtcagaaac
    11101 tgtgatcgtc ataaaaaact tgtattagaa aggagaaaga atgaataaaa gacgttgggc
    11161 agcagttctg attgctgcag gattatttgt ggtttcgtta gtttcagcta gtttgactaa
    11221 gcctcaggaa aacgatcaaa ttgagggatt gaacagttgg ttatatgggg atgaagaact
    11281 agctccaaat gtgttggaag agggagacag tacgcaaaaa atcgtaaaat tatcagtaga
    11341 tggaacgatt gctgcgggag ggtcttcagg tttatttgct tctgaaggtt acaatcataa
    11401 aaattttatg gaacaactga aggcgattga agaagatcca acagtcaaag gtatcttttt
    11461 agaagtaaat tctcctggtg gcggtgtcta cgaaagtgcg gaaatcgcta agaaattgga
    11521 tacgattcgt aaagaacatg atatcccaat gtatgtcagc atgaaaaaca tggcagctag
    11581 cggtggctac tatatttctg ctcaagcaga taaaatcttt gcaacagaag aaacagtgac
    11641 cggctcaatt ggtgtgatca tgtcaggatt gaattattca ggacttctag aaaaactggg
    11701 tgtggaagat acaacagtta aaagcggagc gttgaaagat atgggttcag gaacgcgacc
    11761 agagacaaaa gaagaagaag cggtgcttca agcatatatc gacagcgcat atagtcgttt
    11821 tgtagcaatc gtcagcgagg gacggaataa atctgaagaa gaagtcaaaa aaattgctga
    11881 tggaaggatt tatgatggtg tccaagcaaa agaagtaggt ttagtcgatg aattgggatt
    11941 tcctgaagat gctttaaaag ctatacgaaa ggaacaacag cttgaagatg cggaactagt
    12001 agaatattct tccacgccat ctgggtttgc tagtacatgg ttaggaagta aactagcaga
    12061 attccaggga ttgaagcctt ctgagactag tcagatctta tccgttcttg agaatctagg
    12121 gacagcggaa tcaccaaaag caatgtatta ttacggaggt gagtaagcat gtcagagctt
    12181 tcaaatgaaa ataaggaaca agaaaaagat aagcaacagc agcctgctgt aggacctgac
    12241 tccaactttt cccaaaaggt actgagctct tttgaaagag agcctttgac gcccgaagaa
    12301 atcaaaaaga aacaacgtaa atggaaaaag tatacggaca caagaaatga accgccaaaa
    12361 gcaaatgatt ttcctaagta tttctatgca ggttttttta tccgaatgtt tgccttcttg
    12421 gtcgatcttt tgtgtatcgg agctattacg cgtttaacac ttggtattgc caaaaatcta
    12481 ggttggctta atttttcaga ttcttatctg agtctctacg gattcacagc cttacttatt
    12541 tatctagcgt attttatttt attgacgaaa ttcaatcatg gccaaacgat tgggaaaatg
    12601 atctttggta ttcgagtcat cagctttgat gaagaagaac ttagctggtc aaccgtttta
    12661 gttagagaag gtgcttgccg gttcattcta aaatttcctt tgctgatgtt agggtatctt
    12721 ccagcagtct ttaatcagaa aaagcaacat ctgggcgatt ttttcactaa tacaagtgtt
    12781 gttacgatca atctgatcaa agcgttcaac caggaagtca acagctgatg agcaataaaa
    12841 agcagtaaca gttgaaagca ccttctaaaa gtcagatcta aaatctagct tttagagggt
    12901 gcttcagtta tttttaattg tcatgctttt tttgttttgc tactattgaa catgagctga
    12961 atatgagcaa gaggaggaca ctgcgtgaag ccgattgatt ttccagaaaa atatgagaat
    13021 ttaatgcgag tggcacaaca agcactagca aaccagcaat atcagcaggc aaaagaatta
    13081 tttcaacgag cttacgagtt gaaagaaagc tttgaggcta atagcctgct tgttttttgt
    13141 ctttacgagt tggacgagaa aaaagaagca ttgaagcaag cactcttgca tgaaaaacag
    13201 tatcttcaaa atgaggagtt tgctgaattt tattttgact tgttaatcag tacacaagat
    13261 tttttatatg ctagaaaatt gatagcaagt acagattttt atgaagcttt cgagcagcgg
    13321 gtcattgaaa aaatccaatt tgcagaagaa ttatccggac aaatggagag gcagaagatc
    13381 aaggcactcc acaataaatc ggaggaactt ccttcgttgg aacctgcacg tcagcttgcg
    13441 tctattgatg agatcgaaca gcttccttat catgagttta tccaaacagc gagtaaactt
    13501 attgtatcgc cagaggtcca tattttagca agggcaaaat tactggagac acttaggcaa
    13561 ttgaacgaat gccatcctgt tttctatttg acgattgaag aaaagttagt caaagtcata
    13621 ccaaaagatc tgccaaagcc gcagcagcag gcaagttacc agcaattatg tgtattttca
    13681 gatcgttacg ggaatgaaga tgcgctgctt agttccgtgt taaaagaaga attcacgttg
    13741 caatctgcaa ttgtttatcc tgtctatgat acatacatcg aagacccgaa gcgctggttt
    13801 caactgactg tagaggcgtg tacaggaaaa acgatgtatg aaggtacaga agatgaaaaa
    13861 caagattttt taaaaaaaag agaaaaaatt ttgcagcaaa tgatcttctt ccattagatt
    13921 ttatcagtga aaaagataaa aatgttatga aaaacaagga ttctatccat tcaatatgtt
    13981 tacaatttga aagggttagt gtactattta aaggtatgca tgaaacgttt ttgatgtata
    14041 aaatgaattt ctaaactcgg agggaattaa atgactgcaa catttgaaaa aaaaggcacg
    14101 aatgatggtg ttttaaaatt tacgattgcg caagacgaaa tccaaaaagg attaacaact
    14161 gcgtttaata aagtgaaaaa aagcttgaac gtacctggat tccgtaaagg gaaagtttct
    14221 cgtcaagtat tcaaccgtat gtacggtgaa gaagctttat atgaagatgc attgaatgct
    14281 gtattaccag aagcttatga agctgctgtc aaagaagctg gcatcgatcc tgtttctcaa
    14341 ccaaaaatcg atgttgaaag catggaaaaa ggtcaagact gggtaatcac tgctgaagta
    14401 acagtgaaac cagaagtaaa attaggacaa tataaagatc tagttgttga aaaacaagac
    14461 cgtgaagtaa cagatgctga cgtggatgca cgtatcaaac gtgaacaaga agcacaagct
    14521 gaattagtta tcaaagaaga tgctgcagct gaaaatggcg atacagtagt aatcgacttc
    14581 gaaggctttg tagatggcga agcgtttgat ggcggaaaag gcgaaaatta ctcacttgaa
    14641 ttaggttcag gctcattcat tccaggtttt gaagatcaat tagttggtca taaatcaggt
    14701 gaaagtctag atgtaaatgt aactttccca gaagattatc aagcagaaga tcttgctgga
    14761 aaagaagcag tcttcaaagt gacaatccat gaagtaaaag caaaagaagt gcctgaatta
    14821 gatgacgaat tcgcaaaaga tgttgatgac gaagtatcaa cattagacga attgaaagaa
    14881 aaatatcgca aagaattagt agaaacaaaa gaaaaagcag cggacgatgc aaaagatgaa
    14941 gcagcgatcc gtatggctgt tgaaaacgca gaaatcgttg aattgccaca cgtgatggtt
    15001 catgatgaag tacatcgttc aatggacgaa ttcttgaaca acatgcaacg tcaaggtatc
    15061 tctcctgaaa tgtactatca attaactggt tctacagaag aagacttgca taaacaattc
    15121 gaaggtgaag cagaaatgcg cacaagaaca aatcttgtga tcgaagcaat tgcagctgct
    15181 gaaaatcttc aagcaacaga agaagaaatc aatgcagaaa tccaagaatt atcagaaaca
    15241 tacaacatgc caatcgaaca aatcaaacgc gtgttgacag aagatatgct ggaacatgat
    15301 gtgacaatga aaaaagcaat cgaattgatc acaggtacag cgacagaagc gaaagaatag
    15361 aaatcaagta ataaatcatt tctgaagaag ctacttctag aaaattgttt attcgaaata
    15421 agggactgtg acacgatttt gtcacagttc cttatttttt gttaagagta aaaaatgcaa
    15481 aggtataggt cagttgatcc tttgttaaac actttttctt tttactaaac tatgttatga
    15541 tagtaaaaga ctgtaaagtc tttgatagtt tcttgcatac tttttgcaag ttaaggggtg
    15601 aagaccatgt atgacaatcc aagtagcaat gagaccgtac gctgttcttt ttgcgggaaa
    15661 acccaagaag aagtgaaaaa gatcgtagct ggtccagggg tctacatctg taatgaatgt
    15721 atcgacctat gtaaagaaat cattgatgaa gaattttatg aagaagctgt acgcgaattc
    15781 actgaagtcc ctaaaccact agaaattctt gaagtattga ataactacgt gattggacaa
    15841 gatcgcgcaa aacgctcgtt agctgttgcc gtatacaatc actataaacg tgtcaatcag
    15901 caagcacaag aaaacacaga tgatgttgaa ctgcaaaaaa gcaatatctg cttgatcgga
    15961 cctacagggt ctggtaaaac atttttagca caaacattag caaaaacatt gaatgtacct
    16021 tttgcaatcg cagatgcgac aagtttgaca gaagctggtt atgtggggga agatgtagaa
    16081 aacattttgc ttaaattgct tcaatctgct gattacaatg tggaacttgc agaaaaaggg
    16141 atcatctata ttgatgagat cgataaaatt gcgcgtaaaa gcgaaaatgt atctattact
    16201 cgtgacgttt ccggcgaagg tgtccaacaa gccttgctga aaattctaga aggaactgtt
    16261 gcaagtgtgc cacctcaggg cggacgtaaa catccacatc aagaatttat tcagattgat
    16321 acgacgaaca tattgttcat cgtaggtggt gcatttgatg gcatcgaaac gattgtgaag
    16381 aatcgtatgg gcgaaaagac gattggattt ggtacacaaa accaaaaact ttctgaagac
    16441 gaaagtgtca tgcagcatat cattcctgaa gatttactaa aattcggatt gatccctgag
    16501 tttatcggac gattacctgt tatggcagct ttagagaaat tgacaacaga tgatctggta
    16561 cggatcttga ctgagccgaa aaatgcgcta gtcaaacaat atcaaaaact tctttctcta
    16621 gataataccg aacttgaatt cgaacctgaa gcgttgagag ccattgcgaa aaaagcaatc
    16681 gaacgcaata ccggtgctcg aggacttcgt tccatcattg aagaaatcat gatggatgtg
    16741 atgtttgata tcccttcaaa tgaaaacatc gaaaaagtga ttatcacaaa agaagcagct
    16801 gaattgtctg gtgagccaac agtaatctat aaagattcag agaaaaaagc gggttgatga
    16861 aaagaggctg ccgtaaaagt cattttggct tttgcgacag ccgcttgctt tattcatcac
    16921 cttgttaagg agagagacca tgaaagtcca tcaagctgaa attatcataa gtgcagtggc
    16981 accaaaacaa tatccagata cacaattgcc agaaatagct ctggcaggac gatcaaatgt
    17041 agggaaatct tcttttatca acacattgat tgatcgaaag aatttagccc gtacttccgg
    17101 taagccagga aagacccaga cattgaattt ttatttgatc gagaacgcat tacattttgt
    17161 cgatgttccg ggatatgggt atgcaaaagt atccaaaact gagcgagcaa aatggggaaa
    17221 gatgatcgag acatatttaa cgactagaga ccagctacgt gcggtcgttt ctttagtaga
    17281 tttgcgtcat aagccttctc aagaagatat ccagatgtat caatttttga agtattatga
    17341 tatccctgta attgttgttg ccactaaggc agataagatc cctcgtggta aatggaacaa
    17401 gcatgagagc gaaatcaaaa aagcgttgga ttttgatcgc aatgacacgt ttattctctt
    17461 ttcttccgtt acaaaacagg gaaaagaaga agcgtgggca gcaatcgaac aagcaattga
    17521 atcataaaaa gaataaaaaa gacttaccag tcaaactctt tcataaaagt ttggctggta
    17581 agtctttttt tgagcttaac ttacttttgt ttcttgtctt ttttcttttc attttgtggc
    17641 tgtggtttta gaaatcgatc gatctcttct tttggtgcaa ctgttttttc cggatcgaca
    17701 gcaaatgatt cgaaaagttt atctaaatta tctgtacgtt caaatttcaa ccccattgta
    17761 aacacctctt tgtttctttt tacttagtct atcatgtaat cgtccaaatt ggtttctaag
    17821 acataaagtt ttagtttttc tttatcattg gttataatta aattataaaa agatttgaaa
    17881 gaggtgagaa aatgattggc gaagataaat tattgatgac tatccagctc acagacttaa
    17941 atgaagaaga aaaaaaacag gaaataagcg attgggctat tttgacacgt ctgttgatcc
    18001 agccaacatt tatccgctct ttcacacaac aagcggatcc gtatgatctt cgaaagcttc
    18061 aagaaaaaat aatgctagca tcggtaagag acgaaagttg gttagttgta ggaaatgacg
    18121 agaacgaatg tagttttcgc ttggaagata atcaattatt gataaaaaat gtattgtcta
    18181 tctctgtttt caaagaaaag caatttttga ttcgagactt cattcagaaa aaaatgattg
    18241 ctcatggggt gttcgcctat atgcgagctt atagcgagtt tatctatcac aatacaaaac
    18301 agatctctca acggctattg tttgagaaaa aggatgaaat cgaacattta cctaaaatga
    18361 aacaacaaaa cggggaagta gtagtcgatt gcaaccaatt tccaggatac gatctttttt
    18421 accaaggttt atgtttcact tcgtgttggg aaatgtatta tagtcgctat tatcatcaaa
    18481 tcatccctaa accgattttt ctcgacatcc aacaagtaga aaaagtgaag gaattagaaa
    18541 acgaagtcat atgtatccag ctttatcgag acccctttaa ctggcaaaag ccaaacaatc
    18601 aaatgtttca atcctatttt agggaccagc ttgggtttga tcatcttgct tgggacaatg
    18661 gtgtcggttt attgaaaccg ccattcgttg aatacattta cactgaccat acgatccaat
    18721 ctgtccagta tcagaatgca caaatgcaac ctgttcctaa aaagaatgct tcattttttg
    18781 taacaaaaag ttatgatatc caacaaggag attataaaga aaggcgtgtt cgcggaacac
    18841 taaatgctca ggcgtatttt ccttgggttg acgagaatcg atcacgcatg atgtgctata
    18901 aagttatcga tccgactgtt gctttggata atggaatcga agcttattgt tattatatta
    18961 gggaatatct agaagtagag gtaacagatg aaaaatatca agagtatctt ttatccttaa
    19021 gaatctatgt tccttcagaa tccctgtcag aattaccgtt aaaggaaatc aaacatagac
    19081 tctctgatgt taccttcaaa cgaataaaga aaaagcggcg aagtatccaa ttcgatgtca
    19141 agaaaggtga gcagcatctg cgtgtgcaat ttttagatta ccgagaatta gaacaattga
    19201 ttactttaca aaaaatatga ggtgaagaaa atggataaac aactctatta tcgaataatg
    19261 gttccggcat cattattatc cgtctgtctc ttttttgttc ctaattttcc tattcgatca
    19321 gtcatcggtt ttttggtcat gtttggcggg atgggacttt attattatct tcttcataag
    19381 gaaagaaaac ctgcttctgt ttatatgaaa aaaacaagaa gcgaaaataa aaaacgataa
    19441 aacggcttga tgatagacaa aaaaataaaa aaccagtctt tctgactggt
//
LOCUS       DAFELO010000051        19414 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFELO010000051
VERSION     DAFELO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.052"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            99..1025
                     /locus_tag="LP452_001984"
     CDS             99..1025
                     /locus_tag="LP452_001984"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL2014690.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            1207..2100
                     /locus_tag="LP452_001985"
     CDS             1207..2100
                     /locus_tag="LP452_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL2014691.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            2234..2359
                     /locus_tag="LP452_001986"
     CDS             2234..2359
                     /locus_tag="LP452_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2014692.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(2493..3125)
                     /locus_tag="LP452_001987"
     CDS             complement(2493..3125)
                     /locus_tag="LP452_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014693.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(3273..3815)
                     /locus_tag="LP452_001988"
     CDS             complement(3273..3815)
                     /locus_tag="LP452_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL2014694.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3950..4339
                     /locus_tag="LP452_001989"
     CDS             3950..4339
                     /locus_tag="LP452_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014695.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            4343..5107
                     /locus_tag="LP452_001990"
     CDS             4343..5107
                     /locus_tag="LP452_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2014696.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQAGEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            5236..6183
                     /locus_tag="LP452_001991"
     CDS             5236..6183
                     /locus_tag="LP452_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2014697.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNQYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            6313..7053
                     /locus_tag="LP452_001992"
     CDS             6313..7053
                     /locus_tag="LP452_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014698.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(7344..8012)
                     /locus_tag="LP452_001993"
     CDS             complement(7344..8012)
                     /locus_tag="LP452_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2014699.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            8146..8433
                     /locus_tag="LP452_001994"
     CDS             8146..8433
                     /locus_tag="LP452_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014700.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8849..10387
                     /locus_tag="LP452_001995"
     CDS             8849..10387
                     /locus_tag="LP452_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL2014701.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            10384..11349
                     /locus_tag="LP452_001996"
     CDS             10384..11349
                     /locus_tag="LP452_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014702.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            11336..12658
                     /locus_tag="LP452_001997"
     CDS             11336..12658
                     /locus_tag="LP452_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2014703.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      12721..12825
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12899..13642
                     /locus_tag="LP452_001998"
     CDS             12899..13642
                     /locus_tag="LP452_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014704.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            13657..14649
                     /locus_tag="LP452_001999"
     CDS             13657..14649
                     /locus_tag="LP452_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2014705.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            14661..15398
                     /locus_tag="LP452_002000"
     CDS             14661..15398
                     /locus_tag="LP452_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014706.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            15388..16110
                     /locus_tag="LP452_002001"
     CDS             15388..16110
                     /locus_tag="LP452_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL2014707.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(16194..16817)
                     /locus_tag="LP452_002002"
     CDS             complement(16194..16817)
                     /locus_tag="LP452_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2014708.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            17212..17903
                     /locus_tag="LP452_002003"
                     /pseudo
     CDS             17212..17903
                     /locus_tag="LP452_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            18111..18401
                     /locus_tag="LP452_002004"
     CDS             18111..18401
                     /locus_tag="LP452_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014709.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         6680 a         3198 c         3912 g         5624 t
ORIGIN      
        1 atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc gtttttttgc
       61 tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt ttagtttttg
      121 gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca cacttgcaaa
      181 atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa gaaacacaat
      241 acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct aaagcagaaa
      301 atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca gatatcagcg
      361 aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa acagctaatc
      421 cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta aaactatata
      481 aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca gctatcattt
      541 cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca gctgcaaaag
      601 aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg ttgaaagcag
      661 gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca aaaagtttcc
      721 caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt aatgaagtga
      781 tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca aatggctacg
      841 atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta cttgttgctg
      901 gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac aaccgtgctt
      961 tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg gctgtattta
     1021 gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg atataaaaag
     1081 aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa ttatgactct
     1141 tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat agaaaagaga
     1201 acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact gaccgatgat
     1261 ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga aacgattcta
     1321 agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga tttattgatc
     1381 gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc aatatttgat
     1441 cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga acaattgaag
     1501 agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc gtattgtttc
     1561 gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga gacagaattg
     1621 ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc tatagaacac
     1681 ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc tgtaacgaag
     1741 gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt ttttgatcaa
     1801 atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg gcctagtatt
     1861 ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt cacattagaa
     1921 ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga tccttcattg
     1981 acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag caagaaattt
     2041 ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag taaaacctaa
     2101 gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc ttcctttatg
     2161 gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca ctacatggtc
     2221 tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg aagtagtgat
     2281 aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg gcattttatg
     2341 ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca ctaagaaacg
     2401 aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt gaagtgttta
     2461 aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt tttattttgt
     2521 ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat atccaacaga
     2581 tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag gacaaaaaag
     2641 gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag cggacatttc
     2701 aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat cacgactaaa
     2761 ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag aattgctaaa
     2821 aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt accaggatgt
     2881 cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag agaagacaaa
     2941 atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa aagcaaccag
     3001 cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag aaaaaataag
     3061 atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct acgttgaatt
     3121 tccataatat ctccaaatta aattgataag tatataaagt ataataacaa attgatgagt
     3181 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
     3241 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
     3301 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
     3361 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
     3421 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
     3481 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
     3541 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
     3601 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
     3661 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
     3721 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
     3781 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
     3841 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
     3901 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
     3961 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
     4021 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
     4081 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
     4141 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     4201 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     4261 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     4321 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     4381 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     4441 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     4501 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     4561 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     4621 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     4681 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     4741 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     4801 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     4861 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     4921 tggacaagct ggcgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     4981 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     5041 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     5101 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     5161 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     5221 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     5281 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     5341 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     5401 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     5461 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     5521 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     5581 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     5641 gaaaattctg aaggatatga ttatgtagac agcgataatc aatatggtac agcaaaagca
     5701 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     5761 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     5821 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     5881 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     5941 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     6001 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     6061 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     6121 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     6181 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     6241 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     6301 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     6361 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     6421 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     6481 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     6541 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     6601 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     6661 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     6721 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     6781 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     6841 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     6901 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     6961 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     7021 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     7081 agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa agtttatatc
     7141 gtatcctcaa atttttttga gtttaagcga attttttctc ccaataagta agaaaataat
     7201 ggcaaaataa aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta
     7261 taaagaagat tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta
     7321 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
     7381 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
     7441 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
     7501 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
     7561 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
     7621 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
     7681 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
     7741 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
     7801 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
     7861 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
     7921 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
     7981 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
     8041 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
     8101 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
     8161 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
     8221 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
     8281 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     8341 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     8401 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     8461 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     8521 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     8581 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     8641 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     8701 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     8761 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     8821 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     8881 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     8941 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     9001 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     9061 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     9121 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     9181 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     9241 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     9301 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     9361 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     9421 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     9481 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     9541 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     9601 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     9661 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     9721 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     9781 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     9841 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     9901 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     9961 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
    10021 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
    10081 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
    10141 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
    10201 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
    10261 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
    10321 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
    10381 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
    10441 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
    10501 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
    10561 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
    10621 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
    10681 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
    10741 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
    10801 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
    10861 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
    10921 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
    10981 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
    11041 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
    11101 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
    11161 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
    11221 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
    11281 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
    11341 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
    11401 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
    11461 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
    11521 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
    11581 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
    11641 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
    11701 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
    11761 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
    11821 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
    11881 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
    11941 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
    12001 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
    12061 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
    12121 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
    12181 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
    12241 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
    12301 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
    12361 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
    12421 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
    12481 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
    12541 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
    12601 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
    12661 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
    12721 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
    12781 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
    12841 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
    12901 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
    12961 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
    13021 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
    13081 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
    13141 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
    13201 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
    13261 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
    13321 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
    13381 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
    13441 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
    13501 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
    13561 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
    13621 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
    13681 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
    13741 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
    13801 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
    13861 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
    13921 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
    13981 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
    14041 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
    14101 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
    14161 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
    14221 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
    14281 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
    14341 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
    14401 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
    14461 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
    14521 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
    14581 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
    14641 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
    14701 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
    14761 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
    14821 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
    14881 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
    14941 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
    15001 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
    15061 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
    15121 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
    15181 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
    15241 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
    15301 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
    15361 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
    15421 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
    15481 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
    15541 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
    15601 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
    15661 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
    15721 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
    15781 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
    15841 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
    15901 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
    15961 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
    16021 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
    16081 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
    16141 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
    16201 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    16261 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
    16321 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
    16381 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
    16441 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
    16501 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    16561 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gtaaatcagg
    16621 ttaatatcag aaattgaatt tgattctgca atggcattga tcaaggagtt atcaccaacg
    16681 tatttttgag aaatcgttga tagagtatca cctgattgaa cggtataagc ttcgtccgca
    16741 tgagcagtcg ttcccatgaa gaatgcagcg ccggcagcca aagttgttcc aaaaagtaaa
    16801 gttttaagtg atgtcatgaa aaatctcctt tattcgttaa aaagttttat ttgttgtgtt
    16861 caacgaggaa tactttaaca cgtgaaaata tttcataggt aaaagaagca tgacaattta
    16921 tgacaaatcc gcgttttttg taataaatac agtaatctat gaaatgaaaa gggttacgtt
    16981 taataaaact tgcttttttg tttttgttca ccataaatat ttatttaaga aagtattaac
    17041 ctgcattttt ctctataaaa aagatttttt aaatagataa aaaaggagga ttaaatgagt
    17101 atttgatagt atttatatta aaattacata tgtaatttaa aaacgcttga cattgtgttt
    17161 gagatcactt acttttagca tgtaaaagta ttggattaag gagctatgca aatgaaaaag
    17221 aaatttatta ctggtgggat tgtcgttgca tttctatgtc taattggcta cacaggcacg
    17281 cggtacgcaa atgaaattat tttgtggggt ggagagacag atattgatac aatcaatgag
    17341 aatctgacac agcttgatca tacattgaca gataaagagc aagcgattac agatcttacc
    17401 gctcgactag cacaaaaaga acagacattg caagattcaa aggcagatgt ccaagaatac
    17461 caaacaaaag taaatgaatt agaaaaccaa aaaaatcaat cggcagcaga aaaacaaaac
    17521 ttagaaagtc aattagtcag caaggatgca caactacaaa gcaaacaaaa tgaaatcaat
    17581 gtcaaactag atgagatcaa ccaaaaaaat gatgaaatca atcatctgaa tgcagattgg
    17641 acagcaagac tacaagaagc acaagcaaaa ctttcagaat tacaaaatac tgtaaatggt
    17701 ttgaatcaac aaatcgcaac actgacacaa gaaaaaacaa atgtagaaat cagttaacag
    17761 atacacaaaa gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc
    17821 aaaagttaga aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg
    17881 ttgaagaaca tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc
    17941 aacagtaatc tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag
    18001 tgccaatatg attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc
    18061 ttctttacaa agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa
    18121 aatatggaac agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt
    18181 tcagtttaaa tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag
    18241 tagacgaagg gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg
    18301 tgagatccta tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat
    18361 acccaagaaa aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca
    18421 aaaatgtcct agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca
    18481 cgagcatacg ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg
    18541 atgaatcact aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca
    18601 attcgaagcc ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag
    18661 tcattactag tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt
    18721 atgtaactcg aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt
    18781 cacacgagaa cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga
    18841 atctatatct tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt
    18901 cgacgaattc tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata
    18961 taatgaaaat ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac
    19021 aattaagtat ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta
    19081 tactctatta ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac
    19141 gaataacaaa gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa
    19201 tatgtatatg ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact
    19261 tggctatttg ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga
    19321 actattctta atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat
    19381 tatgttagga attgtagtat tactttgcaa gtat
//
LOCUS       DAFELO010000052        18362 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFELO010000052
VERSION     DAFELO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.053"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="LP452_002005"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="LP452_002005"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL2014710.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="LP452_002006"
     CDS             1011..2258
                     /locus_tag="LP452_002006"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL2014711.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="LP452_002007"
     CDS             2334..2678
                     /locus_tag="LP452_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014712.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="LP452_002008"
     CDS             complement(2795..3121)
                     /locus_tag="LP452_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014713.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="LP452_002009"
     CDS             complement(3494..4696)
                     /locus_tag="LP452_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2014714.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="LP452_002010"
     CDS             4813..5328
                     /locus_tag="LP452_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2014715.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="LP452_002011"
     CDS             complement(5527..5973)
                     /locus_tag="LP452_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL2014716.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LP452_002012"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LP452_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL2014717.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="LP452_002013"
     CDS             complement(7852..8481)
                     /locus_tag="LP452_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2014718.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="LP452_002014"
     CDS             complement(8465..8929)
                     /locus_tag="LP452_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2014719.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="LP452_002015"
     CDS             complement(9416..10684)
                     /locus_tag="LP452_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2014720.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="LP452_002016"
     CDS             complement(10915..11652)
                     /locus_tag="LP452_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2014721.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="LP452_002017"
     CDS             complement(11719..12288)
                     /locus_tag="LP452_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL2014722.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="LP452_002018"
     CDS             12416..13288
                     /locus_tag="LP452_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2014723.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="LP452_002019"
     CDS             13630..14502
                     /locus_tag="LP452_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2014724.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="LP452_002020"
     CDS             14499..16457
                     /locus_tag="LP452_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2014725.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="LP452_002021"
     CDS             complement(16708..17559)
                     /locus_tag="LP452_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2014726.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="LP452_002022"
     CDS             17682..18149
                     /locus_tag="LP452_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL2014727.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
BASE COUNT         5559 a         3757 c         3383 g         5663 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgatg aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tacttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tt
//
LOCUS       DAFELO010000053        18250 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFELO010000053
VERSION     DAFELO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.054"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(35..601)
                     /locus_tag="LP452_002023"
     CDS             complement(35..601)
                     /locus_tag="LP452_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2014728.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(601..1557)
                     /locus_tag="LP452_002024"
     CDS             complement(601..1557)
                     /locus_tag="LP452_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL2014729.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1557..2213)
                     /locus_tag="LP452_002025"
     CDS             complement(1557..2213)
                     /locus_tag="LP452_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL2014730.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2583..4601
                     /locus_tag="LP452_002026"
     CDS             2583..4601
                     /locus_tag="LP452_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2014731.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4671..4877
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5028..7442
                     /locus_tag="LP452_002027"
     CDS             5028..7442
                     /locus_tag="LP452_002027"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL2014732.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7790..9343
                     /locus_tag="LP452_002028"
     CDS             7790..9343
                     /locus_tag="LP452_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL2014733.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9724..11508)
                     /locus_tag="LP452_002029"
     CDS             complement(9724..11508)
                     /locus_tag="LP452_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014734.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11523..13244)
                     /locus_tag="LP452_002030"
     CDS             complement(11523..13244)
                     /locus_tag="LP452_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014735.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13344..14105
                     /locus_tag="LP452_002031"
     CDS             13344..14105
                     /locus_tag="LP452_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2014736.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15072..15340
                     /locus_tag="LP452_002032"
                     /pseudo
     CDS             15072..15340
                     /locus_tag="LP452_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16012..>18250
                     /locus_tag="LP452_002033"
     CDS             16012..>18250
                     /locus_tag="LP452_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014737.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLT"
BASE COUNT         5984 a         3258 c         3692 g         5316 t
ORIGIN      
        1 gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata
       61 aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct
      121 gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac
      181 catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca
      241 tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc
      301 ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt
      361 cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac
      421 gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga
      481 tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat
      541 cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca
      601 tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag
      661 ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa
      721 ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc
      781 cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc
      841 atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc
      901 ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc
      961 gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc
     1021 cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc
     1081 aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag
     1141 gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac
     1201 acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt
     1261 gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat
     1321 catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc
     1381 tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt
     1441 cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg
     1501 taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa
     1561 aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa
     1621 gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga
     1681 acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga
     1741 agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg
     1801 taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact
     1861 aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt
     1921 ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac
     1981 cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta
     2041 gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac
     2101 cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa
     2161 aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc
     2221 ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag
     2281 ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt
     2341 aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt
     2401 ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt
     2461 gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt
     2521 aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga
     2581 agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa
     2641 aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc
     2701 cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa
     2761 tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca
     2821 acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta
     2881 gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac
     2941 aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc
     3001 ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa
     3061 atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct
     3121 atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca
     3181 aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa
     3241 acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg
     3301 gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc
     3361 aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga
     3421 tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag
     3481 gtagccagga aaatggctct tcgacgaata attccggtaa caataccaac tcttcaggaa
     3541 atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg
     3601 attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga
     3661 acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt
     3721 cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt
     3781 ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact
     3841 ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca
     3901 atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa
     3961 caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta
     4021 atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga
     4081 gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa
     4141 acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa
     4201 atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa
     4261 gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg
     4321 tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata
     4381 atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc
     4441 aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg
     4501 ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca
     4561 actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat
     4621 aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt
     4681 gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca
     4741 cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc
     4801 ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg
     4861 tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa
     4921 gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta
     4981 tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc
     5041 ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc
     5101 atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc
     5161 aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga
     5221 aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg
     5281 ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg
     5341 aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga
     5401 atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa
     5461 aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta
     5521 tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa
     5581 aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc
     5641 tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt
     5701 cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta
     5761 cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc
     5821 ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga
     5881 cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta
     5941 ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt
     6001 atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg
     6061 actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg
     6121 ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca
     6181 tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga
     6241 aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg
     6301 aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc
     6361 taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat
     6421 tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc
     6481 gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg
     6541 accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg
     6601 acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc
     6661 ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca
     6721 atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag
     6781 tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt
     6841 tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta
     6901 aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta
     6961 tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga
     7021 cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca
     7081 atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac
     7141 tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg
     7201 gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag
     7261 aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag
     7321 gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag
     7381 gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt
     7441 aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg
     7501 aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag
     7561 gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa
     7621 gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac
     7681 tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt
     7741 gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga
     7801 actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag
     7861 agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt
     7921 cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat
     7981 gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt
     8041 gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc gtcatttcga
     8101 tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga
     8161 tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga
     8221 ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc
     8281 aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa
     8341 aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt
     8401 tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc
     8461 ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca
     8521 aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa
     8581 gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg
     8641 tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct
     8701 gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga
     8761 tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa
     8821 tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa
     8881 agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc
     8941 agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag
     9001 tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt
     9061 ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt
     9121 tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga
     9181 agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc
     9241 aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg
     9301 gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag
     9361 cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt
     9421 gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata
     9481 gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt
     9541 tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg
     9601 agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca
     9661 tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt
     9721 ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg gtgattggag
     9781 tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc
     9841 tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac
     9901 agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc
     9961 atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg
    10021 ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag
    10081 tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt
    10141 ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt
    10201 ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag
    10261 accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa
    10321 agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga
    10381 aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa
    10441 agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc
    10501 tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt
    10561 gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc
    10621 ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact
    10681 tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac agttcataaa
    10741 ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc
    10801 atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg
    10861 attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc
    10921 aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt
    10981 gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa
    11041 ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact
    11101 aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt
    11161 tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc
    11221 ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa
    11281 caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc
    11341 tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt
    11401 cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt
    11461 ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct
    11521 ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct
    11581 tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt
    11641 tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga
    11701 agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat
    11761 acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg tttctggccc
    11821 cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc
    11881 actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt tgaggcatat
    11941 tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc cttctgaggg
    12001 acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg aataccacca
    12061 agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc caaactactt
    12121 tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat tgtgaaatca
    12181 atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg ttcaaaagca
    12241 gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc ttcctcaatt
    12301 tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga tttagggata
    12361 atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga gtattcgact
    12421 acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc gccaaaccac
    12481 aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat
    12541 attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt cgttcgcttt
    12601 tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa gaattctctc
    12661 aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg tgcccctcgt
    12721 ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt aacccatgtt
    12781 agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac cggtgctggt
    12841 aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc attcgtcatt
    12901 cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag ctgttggaat
    12961 ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa tgctgaaaag
    13021 taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat ttcaactcca
    13081 atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag ctttgctact
    13141 aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa ggtaaggaga
    13201 gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt cctccttttt
    13261 tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga aaaaagaata
    13321 aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa gtatcggaac
    13381 tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta atccgttcaa
    13441 gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt atgctttatg
    13501 atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat ttatatggaa
    13561 aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc cataaagaac
    13621 tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag ttgaaattga
    13681 tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac tgtatgatct
    13741 caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat tcaagctttg
    13801 ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt atcgacccta
    13861 gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg gctgtttatc
    13921 gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt ttagaaatca
    13981 gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag tatctattga
    14041 cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata gaaatgaaaa
    14101 aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt ataaagaaaa
    14161 attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat agatagaatt
    14221 ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct tagcgatata
    14281 tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat
    14341 aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga gtgagattgt
    14401 atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa aatacattgt
    14461 tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt gaataaacat
    14521 attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat actaccgata
    14581 gcattttttt gcttataaaa taattataag tatttagaga tttttcttct aacattcttc
    14641 aaacaagaaa aatcacaaag gttattgtta tttttatata ttctttttaa gaatgattgt
    14701 ttttttatag aaattttaaa aaataaaaca cgttattgat ggatagttag tttgttattt
    14761 ggtaatatta tagaagttga ctggtagtga tttatttttc gtcgttttat aaaaatgtta
    14821 ttttttggaa aaattcacaa atatgtaacg aatgtttttc tggaatttgc tgtgttaaaa
    14881 agcagtatga ctaatataaa tactattgtc atttatgcaa ttttttatgg atagtaattt
    14941 tagaggggaa tcaatgaagg aagaatagaa gggaaaaata gttgcaaata cgccagttag
    15001 caaaaatagc aatacatctt caagctttaa aattcgagag aatcatcttg gtagtggaat
    15061 caactacaac aaaaggatta aaccaattaa tagatagaat taccaatgga aaaatagaaa
    15121 aggtagttat tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt agtgaaaccc
    15181 tttgcaatat atggtacaga atatagaaat tattgacatt acagaaaatg atgaagattt
    15241 aatacagatt gtcactattt gtagatacct tttacaagga aaccgagcca ataaagctaa
    15301 gaagatgatt aaggagttga tggaagacga tacaagttaa gaaagtgaaa ctacgcccaa
    15361 ataaagagca aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca tcgattcggt
    15421 ggacagttta ttagtaatga cggtttgaga acatccatga cgagaatgaa ggaacatcct
    15481 aaatgcgttt agttgaacca agtgccaaat aatatggcca aataagcgat cagttatcga
    15541 tgaatcccaa gtattctaaa ctatatattc attttgatgc taagcattga tatatttttt
    15601 tgggattgat gaaggttaac caatacaggg actcactgat gaaattctag agtctgacct
    15661 tggtatcaac gatttagtga ttgtttctaa tggaaaagta taaaagtata taagtacatt
    15721 aacaaaataa ctattagggt tacgctccag cttgtgcaag gcactggcag gaggaatcgt
    15781 gaaatcataa ttatggttaa gccacacgga caactaaaag tcatacaaag ctatcgaatc
    15841 aatcaacaga agacaatcgg caatcattag ctttgtttac agcttacatt gcaacgcttg
    15901 agttgaaccg aaaatacgag ggaggtaacg cagccatttt gagttgggga atctttttag
    15961 aaaattatcg ggtaccttgt tgtttagtca aaattataag ggggagtaat agtgaggaac
    16021 aaatttcata aactgttaag tatgtttcta gttatgctta ttgtgatcaa tcaatttggg
    16081 aacataacgg ttctagctga gtctttagca gaacagccag agcgttcgct gacgatcgaa
    16141 gttgatcaag aggaggctgc tttgggtgag cagttccagt tgcgagtcag aggaacacca
    16201 gacctgctaa atgcattggt cattgataag agtgaggatt tgattgaagg ggaagtgaag
    16261 caactcaatg attacgaacg tctgattact tattcttccc agcattcagg taaatacgaa
    16321 ttgaccgcaa gaagcggtga tattcaatca aatacggtct caatagaaat cagtgtacca
    16381 gaagaaagca caagtgagtc ttttaatgta ccagaagaaa gcacaagtga atcttttaat
    16441 ataccagaag aaagcttaag tgaatctttt aatgaagcca gatcatctga gccatctata
    16501 acgcaggaat ctcagattga aagacaagag gagctcttga attatgacat cggcatctct
    16561 gctgagcgcc ccactttgcc gagcaggcat acacaaacgt taaagggata cattcaagga
    16621 ggcgctggcg atattcttga agaatatggg gagatggtcg tacgagttcc tagagcatcg
    16681 attggcttaa ttgagttcat tagagtggag gtcgctgatg attcaccttt aaagccactg
    16741 gcattttacc aagaaggcac agacgttgtt tttccattta cctttgatta tgaaggatta
    16801 aatgaagccg ctcagtcgta tacattcagt atcacgtttg aagttgcttt ctttcaatca
    16861 ggcggtactg agccaaataa tgactttcaa gtgagtctta cttatgctga gcgagcagca
    16921 aacacggaat tttcaattac gcctattcct tcatcaagac ccattttttc gaaattatac
    16981 tatggctcta gagaaatcga cagccaagga aatcttggga atctgctgat tgacgggcaa
    17041 gacgtgacca acagtaataa atttattttg caggtaaatt atgaaggatc acgaaaagac
    17101 ggtgtagtgg ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa tccgactaca
    17161 tggagttcca ctggaaatag agaacctatt tcaggtgggg ttcggatcgc agaagtaacg
    17221 aatccaggtg aagcaaacgt tagaactcat tatgtttccg aggaatttgt ggacaaagta
    17281 tcttttaata aggaaacgaa tacacttgtt gtggactttg gtaatctagc agagggtcaa
    17341 tcgtatttta ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa tgaaggaaga
    17401 caagaggatg ggttttctcg gaatcgagcg agtatgacct acaataatca ggtaatgagc
    17461 cgagactacc gcttgagaat ccctgaaagt aatcttccgg caatctcttt aagaaaaagt
    17521 gtggacaaaa atgttatcaa tctcaatgaa aatgaactaa cgtatactct gcatgtctcg
    17581 atgttttctg gaacaagaaa tacgcttgtt attgaagatg ttttggaaga aggcatggaa
    17641 tttgtgtcat ttctatcaga tattccctct ttcattgatg ccgaagtcaa taatggaata
    17701 gttcgtttca gtgcgaataa tacgattggt ggtccgacaa gttttaccct ctcttttaag
    17761 gtcaatgtag aggcgtatgc ggtcggcgat caaattttaa actttgcaac gcttgtgaat
    17821 agtgacaatc gattgaatac gagtactgta agaacaagga aaatcgatgg gcgtattaaa
    17881 gtaattaaaa ctgatgagaa tggccaaagg ctggcaggag caaaatttga aattttatca
    17941 agcgattcac aagtgataca agaaggtata acggcagaaa acggagaatt tttaagtgaa
    18001 ccgcttcctg taggtagcta tcaagtccga gaaacagaag caccggaagg gtatctttta
    18061 gatgcgacgg tacatgatgt ttttataaat gaatactcca tagaagaagt gacgttaacc
    18121 attgaaaaca tccttgatac gggtggtgtt gaactaatta aacatgctga aggcagtggt
    18181 gaagtccttc aaggagctgt ttttgagtta cagaaccgag aaggagaaac gctacaaacc
    18241 ggtttaacta
//
LOCUS       DAFELO010000054        17516 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFELO010000054
VERSION     DAFELO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.056"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            99..2426
                     /locus_tag="LP452_002034"
     CDS             99..2426
                     /locus_tag="LP452_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2014738.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2552..2881
                     /locus_tag="LP452_002035"
     CDS             2552..2881
                     /locus_tag="LP452_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL2014739.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2921..3925
                     /locus_tag="LP452_002036"
     CDS             2921..3925
                     /locus_tag="LP452_002036"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2014740.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3922..5351
                     /locus_tag="LP452_002037"
                     /pseudo
     CDS             3922..5351
                     /locus_tag="LP452_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5557..6729
                     /locus_tag="LP452_002038"
     CDS             5557..6729
                     /locus_tag="LP452_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2014741.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6891..7346)
                     /locus_tag="LP452_002039"
     CDS             complement(6891..7346)
                     /locus_tag="LP452_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014742.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7935..8357
                     /locus_tag="LP452_002040"
     CDS             7935..8357
                     /locus_tag="LP452_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014743.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8523..9716)
                     /locus_tag="LP452_002041"
     CDS             complement(8523..9716)
                     /locus_tag="LP452_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2014744.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9940..10317
                     /locus_tag="LP452_002042"
     CDS             9940..10317
                     /locus_tag="LP452_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2014745.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10305..10643
                     /locus_tag="LP452_002043"
     CDS             10305..10643
                     /locus_tag="LP452_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2014746.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10777..11220
                     /locus_tag="LP452_002044"
     CDS             10777..11220
                     /locus_tag="LP452_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL2014747.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11482..12297
                     /locus_tag="LP452_002045"
     CDS             11482..12297
                     /locus_tag="LP452_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2014748.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12307..12969
                     /locus_tag="LP452_002046"
     CDS             12307..12969
                     /locus_tag="LP452_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2014749.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13174..14838
                     /gene="alsS"
                     /locus_tag="LP452_002047"
     CDS             13174..14838
                     /gene="alsS"
                     /locus_tag="LP452_002047"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL2014750.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14851..15561
                     /gene="budA"
                     /locus_tag="LP452_002048"
     CDS             14851..15561
                     /gene="budA"
                     /locus_tag="LP452_002048"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL2014751.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15691..16188
                     /locus_tag="LP452_002049"
     CDS             15691..16188
                     /locus_tag="LP452_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL2014752.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16373..16633
                     /locus_tag="LP452_002050"
     CDS             16373..16633
                     /locus_tag="LP452_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014753.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16995..17234)
                     /locus_tag="LP452_002051"
     CDS             complement(16995..17234)
                     /locus_tag="LP452_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014754.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5942 a         3045 c         3475 g         5054 t
ORIGIN      
        1 aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt agaataacgt
       61 gtaaaggatt atttgatgat tttttattga gaggagggat gaaaatgatt aagaaaactc
      121 aaaataaaat ccaacgtata tgtcaaaaac attttgtttc tagactcaaa gctgtatcta
      181 aaagaaaata cttacatccc attatgattt cgatcatctt gatttttact ttcttaggga
      241 gcttatcaag tatttatgta aacgtttata cagaaacgtc aaattttgct aaccaaacga
      301 cagaggagac aaatagtcca ccctattctg aaatacagtc catagaaagc accaatcagt
      361 ctagcaacaa cgagacaacc tcaaccacat cctcgactca actgattgat ccgacagaaa
      421 aagaaatcac agcagcaaaa gcagcagggg agaaagctta ttcagaaaca ggtagaagcc
      481 agacatttag tactgttgcg gccactgcga atttcaacgg acataccata ccagatggat
      541 atacagtgat atatgccaaa gatggagtca ttgttgcggt taacggatcg atgagtgcgg
      601 ataaaactgc gttacaaggc gtaaccaaag caatcgcttt tattgataac cgtccagata
      661 tccataatct gtttattggc tccacagtaa atggtactac tatcacattc ccaaaaagta
      721 tctatatggg ggtggctacc gctggaaata cgatcgattc agttacaccc aatgcggata
      781 aaagttattc ttttacaccc tcaacatggg gagggcttcc tgccggagga ggtatgagta
      841 ctttttcaat cggtatacag ttagcaaatg atattccatt cacggggtac tcgatcaaaa
      901 atgggcagat cgttgatggt aagggaaatg tttcaaattt tccaacctat caagcagcaa
      961 cggttcaggt caatgcgaat cggacaggaa ctttttctta tacagtcaat ggtggagcag
     1021 caaaaatcgg aacgtacggg actacattat ctggaatcca tgtgggtgac aaagtggtta
     1081 tcactccaac agcagtaacg ggctattctt ttgcacaaac aaacggttct ttcacagccc
     1141 tagaaagtaa cgtcgatacg gtgacctata caggcaaaca aacaccaatc actattaagt
     1201 atcaagacta ttttggacag atgattgctc cttctaaaat aatgaatctt acttacggaa
     1261 gtgctgcaca agacctgacg actaatacgc ctactatcaa cggatatacc tttacagctg
     1321 tttcagcgac agaaacaaag aaccaatcag caatgagtgt tttagctagt ttagatacaa
     1381 atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat ttctgaagtc atcctatatt
     1441 ataagacaaa tgcaacagta tcaatcaatg caaatggcag taagtactat gatggtcttt
     1501 ctgttgtacc gatcgttaaa tatgccttta atgataaaac agaatcaaca tcccttttaa
     1561 acaatgatct cagtattgat caaatcacat ggaatccaac tgatttcaaa gcgatgaatg
     1621 aaaagggagt tgaagtaact gcgcctactg aaattggaac ctatacgtct tggcaattga
     1681 caaaaagcgg actagctaaa ctagctgcca gaacaaacta tttatttact atcgtgcaga
     1741 catctgatgt ctatacgatt aagcaaatca gcggtgcggt agagctcagt ggcagtaaga
     1801 catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta
     1861 gttcgcttac tccagtacaa gtggcactta gcagtatgga ttacacagca gatgcacaat
     1921 cagcaaaaaa tatggttaat gctggcagct atacaattaa actaacgaat agcggaatag
     1981 acaaagtcaa agcagcaaat cccaagttgt catttactga tatagccaac acatcaggaa
     2041 cttataccat ctataaagct aacgcagtga ttactgtcga tgacgctgcc tttaattatg
     2101 atgcccaaag tcactcgatt tcggttggaa atctccacgt taccggtgtt gtcttaggcg
     2161 aatcattgga ttatacccta acagttaaca gccggacaga tattggatca caaatagtaa
     2221 cgattagttt aggcgaagga agtgtaaaca ataattatac gattatcgca acagataccg
     2281 ctaagttgac gatcaatccg actctcttgc ttccttcgac aggtgggata ggtctactgc
     2341 cctttgtctt tttaggactg atctttattt tttctggctt cttttatttt atccatcgta
     2401 agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc
     2461 tgttctcttt tttatcctac tggtcttttt tataggttct ccgactgtgt cagctagaga
     2521 atcacctatt acatttactt tgcataatcg aatgttcgaa agcgataaga tgccaagtat
     2581 cagtcaaaac gacggaataa atatcccgga taactttcaa ggagtaaaag ggttgaacgg
     2641 cgttactttc gctatatatg atgtctcgga tgaattttac aagctgcggt cagaaggaag
     2701 tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt gatagtgaaa aaatactggc
     2761 agaaaatgtg acaaaaacag tagacgagga agagggcatt gcttctttta gtgcatcgga
     2821 caaagatgag caaggtcgtg acgctgttta ccgatttgcg gaaataaaga cttcagatta
     2881 agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct gtgacagatt catctaatca
     2941 taaattgacg accatccact tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac
     3001 attaacaaaa acagtagaga ataagcagac agattttgca gatggggata aagttcctta
     3061 tctcatcacc gtaacaattc ctgagaacat caacgagata ggaacctata cgatcaaaga
     3121 tacagctgat cctcagttat ggctagaacc agaaacgatc gattttttga ttggtggaga
     3181 caagattcat acatttcaca cacaaaaaac aaaacatggt tttgtcttat cagattctgg
     3241 aaaagatctt agttcgtttg ctgggaaaaa attaacgatc cgttaccaaa tgacattgaa
     3301 agaaaacagt gaaaaaacga catttgataa cgatgtacgt ttgacgactg acaatcaaac
     3361 agtagaaaca catcttgcca tccaaacagg aggcaaacaa tttgtcaaag tggatcggca
     3421 aacgaaacaa aaactagcag atgctcaatt tctagtcaaa aaaaatgacc aatacttagc
     3481 gcaagaaaac gggataaatc attgggtagc caaagatgat tcaagagcaa cgatttttac
     3541 ttccagtaaa gatggaacat tttctgtcca tggattgtct tttggaagtt acgaactagt
     3601 ggagattaaa ccgcctaaag gatatatatt gagtcaatca accattctct ttgaagttga
     3661 aaaaggcagt tatgatccgc ttcatcctat accattggaa atcatcaacg aaccaaaagc
     3721 aaatccgacg aataatggaa aaacgccagt tcagatgcag acaaatggtc catcaaatga
     3781 aaaaaatgga ggaagctttc caaaaacaag cgaagagatg cttggaggtt tctcaatttt
     3841 agggttgttg ctagtccttt caacagggac agcttggttt tataaaaaac aacaaaaagg
     3901 aaacaatagg agggaaataa aatgaaaaac atcaaaacaa aaatatggac gtttttagga
     3961 actgctatca tgctgcttcc tttcgttctc ggcctaggaa ctgcagaggt ttctaggccg
     4021 tatcacctac accagaaaat gttacggtta atttgcataa attgaaattc acttcagcac
     4081 cagaaaatca aataaacaac ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg
     4141 gcgttgaatt caacgtatat gacatcactg ctacgtacta tccaagtaaa gacacagcag
     4201 ttccagcaga cgctacaccc ttcgcaagtg taacgacttc tggtgaagga ttagctaacc
     4261 tgactcttcc aggtaaatct gatgggaaag atgctgtata cgtctttgta gaaacaccaa
     4321 agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt acccttttat aacccgaatt
     4381 catcagaccc tgacaaacct ttagacacga ttcacctata tgctaaaaat gtcatcaaag
     4441 aaagcaatat caaaatccag aaagctggga acttttcagg ggtagataag ttagcagggg
     4501 cgcaattcca gatcaaaaat tcagagaatc aattcgtgac cggttttgat gaaaacactg
     4561 gtgtcgctct ctatgatcct gctgagtcag cgttgacatt cacaactgat actgaaggat
     4621 ccatcagtgt caatggatta atagacggca attatacttt agttgaaaca aaagcaccag
     4681 atggttatca attaaccaat ccacaaaaag aaactacttt tatagtaaaa aacggaaaat
     4741 tgcaacaaac aaccggtatc atccatggcg cagatgaagc caatcttggg aatccaaacg
     4801 gtgtagtcaa tcgggaaaat acaactacgg ataacacgat tactgtagaa aatgtccaaa
     4861 actacggtga tttccacttc atcaaacaag atgtgaacac gcaagaaaaa ttagccgatg
     4921 cagaattttc tattctaaga gcaaaagatc caactgttaa attaatgaaa aaaatagcaa
     4981 atcttgcctc aggagaatac ccatatgctt ggtcagatga agtcacaggt tctgactggg
     5041 aagaagtgaa attgatttct gattcagaag ggaaattttc aatcgaaggg ctgaaacaag
     5101 gggcatactg ccttcaagaa acaaaagcac cagaaggcta cgcattacct aagaatgaag
     5161 ctgcgtacac tgaatttaca gtagatgatg atccaacaac ttctgatgaa acaacgatcg
     5221 ataaccaacc taaaggaatc ttgccttcaa ctggtggtat ggggatcatc gtattcatct
     5281 tggtaggtac tgctttagtt ggaggagcag tgatctactt caaaaaacgc catcaagaaa
     5341 cagaagctta aactattgga aacaaaaatc atacgataaa taaacgatgg tgattcaaat
     5401 ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt cgctcaacaa acggcaaaat
     5461 aggaggaact attttgccgt ttgttgagcg aatactcagg tcaaggcgct gatttcacaa
     5521 cctcttcata tacatattag aaaataggtg gacatgatga ataaaagcag aataagaata
     5581 ctggacatct tcatggcaat cattttagtc gttgggatcg ggatcttttc ttatcctttt
     5641 gtagaagatt cactgaatga ttttcttgcc cagcaaatga tcatccatta tcaaaagcaa
     5701 gcatcgaata agaattcaga ggaaataaaa aaacagcagg aaaaaatgac aaagaaaaac
     5761 caacagttgg cagagcaaaa cgtatcacca gggataggca gtttcaacca agcagttgat
     5821 gcaaaagcat taaaagattt acctagtaat gcctttttta tggaacatat gcttggagtc
     5881 atcgaaatcc caaaaatcaa tgtcagctta cctatattcg atcagacaac ggaaatattt
     5941 ttgcaaaaag gaacgtcatt attggaagaa agttcttatc cgactggcgg gaaaaacacc
     6001 catgccgttc tttctgggca tcgtggactc ccagaagcaa aattatttac agatttgcct
     6061 aagttgaaaa aaggagacca gtttttcatc cagatcaatg gaaagacatt ggcgtatcaa
     6121 gtagaaaaaa tccaagtggt cttacctgat gaagtagatt ctttgggtat ccaaaaaggc
     6181 agagatctcg tgactttgct tacctgtact ccttatatgg tcaatacaca tcgactgctt
     6241 gtgacggggc atcgtattcc ttatcaagca aaagaggcaa aaaaagcgat ccaaggaatt
     6301 gatcaatgga aaaaatggaa attttttatt tggtttatcg gtatactact tggatcaatc
     6361 ggcttagttt ggctactcat cgcttattta gattctttgg ccatcgctaa gcgaaactat
     6421 cctttatctt tttatgtaaa gaacacaaac ggacgaccaa tcgaaggaat ggtcttttct
     6481 gtaaaaacgc tcaacggcaa acactatatt actagagaaa aagttccatt tgtgaaagct
     6541 tctgatgaat acggactagt gagattttct gatttaaaag gcaggaacta tcgcttgcaa
     6601 catgaggagc tcctgcttaa aatccatgta aaacataaac attcgaaaca gttttcaatg
     6661 aaattgaaaa aaggaagata taaactgcga aaagaaaaag aggtgtacta tttgattgag
     6721 aaagaatgaa tgaagatgtc caaaaaagca gagggaacag actaaatgaa aaaagagaga
     6781 agctgacgaa atggacgtgt aaactgctat ttttggttta ctatctggaa cgccagcttc
     6841 tattttttta tgtttcagaa gaattttatt attatagtat aacttttgaa ttagtttcca
     6901 taacatttgg agcaaggggt taaaccacga gcttgagctt cgcttaaagt ggcaggggta
     6961 tacgtaccat ttccacattt atgtgtatgg tattttgaac cggtcggtgt gacatagaca
     7021 gtttgttcat tattttgctc aggagctgcg gcttgtgcct gctgagaggc ctgttcagct
     7081 gctgcttgct gtcgcgcctg ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt
     7141 tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt cttcttctgc ttcttttgct
     7201 ttgattgtgg aatcaattgc agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc
     7261 gcttggatag ctgctgaagc agttgcatag ttttcttttg taggatttgc ttcagcttgt
     7321 gacaaagcag attccgcttc tgtcactttt ttttctagtg cagctttttc cgctttttgt
     7381 ttctcttctt cttttttctt ttcaatttgt tcttgttctt tcgatgaatc tgccgtactt
     7441 tcggagatcg tggtactgct gctggaagcc tgagctgaac ttgtgctttg agtagtagac
     7501 gtagtagcac cacacccact caacaaactt cctgcgacca aaaacaatgc aactaatttt
     7561 ttcactaaat ttcctcctta agtaaaaaac aaccgttttc aataaatgtc cttttcaatt
     7621 gtttacttac ttgttataat aattgtgtta tgaatagtga ttgaggaaac ttaatctaat
     7681 cccgttttga tacacgggat tttttgctat agttctgtta ttaagtatgc aataataaaa
     7741 tatgtacgtt attttattat tagattattg acatttatat aataaacgat taaatgattc
     7801 atatcaatag aatacttagg aaaaaaagag aaataataac aggtttatgc actattgacg
     7861 gtgtgacaat atgatatttg tatccgtttt tttcttgtat gtagtatagt aaagtagata
     7921 caggaggaga aaatatgcag acttttttaa caaaagtttc tcaaaagaaa attaaaatga
     7981 ttcatttact gcttgaaaca gaacgttggt gttcaataga agaactacaa aacgaattga
     8041 aggtatcaag taagtctatc ttgaattatc taacagattt agaagaattg ttccaaaaat
     8101 atcctgataa ggtcattttg aagaatgaga ataatcggcg cttttctatg gaaaagcagg
     8161 agaacttccc aatctacatt atttatcttc atttctacag aaaatcatat aattatcatt
     8221 tgattgaatt catgtatcaa catcctgaga aaaatttgga agattatgca gacgctcaat
     8281 atacgagtgt atccacagtt tttcgttatg ctaagttatt agtaaagtac tttgaaatga
     8341 cggacgtcaa agaatagtag ccgccagata gaaggaaatt agcagttgaa aaagctgccc
     8401 tgcatcaagt aatacgacca gccaaacgga aacagtgtct catgtttttg atttggctgg
     8461 tcttattacc gcagatgcaa gcttttgcca cacctctttc ttgtcattta cagactcaac
     8521 tatcaatgct gcaagctttc ccttaatgct gtaacaccag taagagttgt caagaccagc
     8581 agactgacta ggtaaatacc gctcaaaaac aagattacgg cagttaaact ataatgatcc
     8641 atcaagaaac cgatgacgat agaagaaaaa ccaccaacag ctcgtccaag gttcataatg
     8701 aaattattcg ccgtcgcgcg gatttctgtt ggatacaaac tactaatcac tgcgccatat
     8761 ccaccataca tgccattgat aaagtagccg actatcacag cggctgctac caaactccac
     8821 tggctatggg ctaacacaag tagatagaca gataaggatg aacaaattaa aaacaaagaa
     8881 aaggctagtt taggccccaa tttatccatg attatgccaa aagtcatcat ccccaaagac
     8941 atgcctaaaa tagtactgac catccacaat gaagacccag aaaccgaaag ccctaatcga
     9001 ttttgcataa ttgaaggcag ccaattcatt aacccaaaat agccggcaat ttggacagtt
     9061 accatgatac taaggccaca tgtttgccaa gcaagatgtt ttgattcaaa cagtctgcta
     9121 agtttggttt tcttttcggc agattgtaca gatttgaatt cctttggttc agtcaagccg
     9181 cgacgaatga acaagaccat caacaccggc agtacaccta cgacatacaa catcttccaa
     9241 ccaaacgttg gaatgatcac agcagctaaa atcgcagcca aagccgcacc gacctgcgca
     9301 ccaataccag caaccgaaga agctcgtcca atttgttttt ttgaaaaact ttcggaaatc
     9361 aaggacatac acgccccgta ttctcctccc gctcctacac ctgcgataaa gcgaaagaga
     9421 taaaccaagt agatgtttga agcaaaatac atcaataaag aagcaatcga aaacagaagg
     9481 accgtttgag aaaatacttt cacacgtcca tatttatcag ctatgatgcc aaaaaaaata
     9541 cctccaatga gcatccccaa attagtgatc gttgaaatga aacccgcttg cgtaccacta
     9601 atatgaaaag aagtaatcat tgaagataat gaaaaagcta aaaacataat atccatattc
     9661 tctaaagcaa ttcccgccga tgtcgataat aataactttc gctgatacgt tgtcattttt
     9721 ataccccttt tcctaaaatt tccctaaatt gttaaaagta tatccaaaaa agaataaaaa
     9781 aacaagactt ttttatttga aataatacac aaacaaccga tatagataat ccagcttagt
     9841 tttaatagaa atgagtattg acttggagta cacttaaagt tgtagaataa ataaaataca
     9901 taagtaaagt ggcaagaaat taagagaaag agtgatcaga tgaatatcaa acaagtcagt
     9961 gaagaaaagg gcatttcggc cgatacattg cgctattatg aacgaatcgg gttgattcca
    10021 ccagttaatc ggacaaaagg cggaatccgt gattatacag aagaagacct aaaatgggtc
    10081 gactttacga tctgtatgcg aaatgccgga ttatcgatcg agtctttgat tgaatatatt
    10141 cgcctatcta gcgaaggaga agcaactgta tttgagcggc gtaagctttt gattgaagaa
    10201 agtgaacttt tgacgaaaaa aattgctgaa atgcaagaat gtttggaacg tttacaagga
    10261 aaaatcaaga aatatgatcg tgtgttatct agaaataaaa tggaatgtat caagtaaaaa
    10321 aatcaaggag catcgctatg gagttacgct tactaaaata tttttggaca attgcagaag
    10381 aaggaacaat ctctcaagcc gcagaagttc tacatcttac acaaccaacc ttaagccgac
    10441 aactgaagga gttagaagag gaactaggaa cagaactgtt tgtacgcgaa aaccgcaaaa
    10501 tgatcttgac agaagcaggt tattttctaa aaaagccgag cagaagaaat tttagattta
    10561 acgaatcaaa ccactcaaga attcgagaac agaaaaaaac aattattcag cggccgattt
    10621 acgatcggct gtgtggaagc tgataattcg aatacattgg cactattact agaaaagttg
    10681 atccgtgatt atcccagcgt tcaatttcat atccacagcg gaacaagtga tgatatcacg
    10741 gagtgactag aaaaaagcct gttagattta gcgattttgc tagatccagt agatacagag
    10801 aaatacgaaa cgattttgtt accaagaaag gaaaaatggg gattgctcat tttagaaaat
    10861 tctgttttag caaaaaaaga cgtgataaca aaagaagact ttgcgaatgt acctcttttg
    10921 atctcaaagc gtccagaaat acagcgaatg tttgctgact ggttaggcat aaaaaaggag
    10981 cagttgctga tcacaggaac gtacaatttg attttcaatg tatttgcgct aattgagaat
    11041 caggtaggtg ctgctttagt catagaaggt gctgtaagtc atctttcaaa gcgagtgaca
    11101 ttcgttccgc ttgacccaat gattgaaacg aactgcatgc tagtctggaa aaagaatcgg
    11161 attttatcac ctgtagtcaa tgaattgatt caccgtttca aagaagcttt tgaacagtaa
    11221 taagtatctc taatcgaaga aatttcaata caaaagcgag acgtatgaca gtcttgcttt
    11281 tgtatttttt catttttaaa tcaaaaaaaa taaagagatt tacataactt gttaaataag
    11341 cgcttacaaa cataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    11401 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    11461 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    11521 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    11581 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    11641 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    11701 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    11761 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    11821 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacatat
    11881 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    11941 caatactaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    12001 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    12061 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    12121 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    12181 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    12241 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    12301 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    12361 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    12421 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    12481 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaaaagt
    12541 ttttcaccag tacgtttgtt cataaagacg tttcgccaat tttggctaaa gggatttttt
    12601 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    12661 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    12721 tcctttagca tcaactactg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    12781 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    12841 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    12901 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    12961 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt atgtaaaaac
    13021 tgtcattctt tttcattttt tgaaacatat ggctaaacaa atgaaataaa acgtgctata
    13081 cttttgaatg aaacaggttt agaaactgta tcaattattt gtgattattt ctaactaata
    13141 attaaaaata aagcataaaa aggacgatgt tcaatggacg aaaaaatgaa acaaggttcg
    13201 aaatctgtag tagaaagttt gattaatcat catgttgatt atgtattcgg tataccaggt
    13261 gcaaaaatcg atggtgtatt taatgaatta gaggatcaag gtcctgaatt aatcgtgact
    13321 cgtcacgaac aaaatgctgc atttatggca caagcaatcg gacgtattac aggagagcca
    13381 ggagtagtta tcgcaacaag tggtcctggt gctagtaact tagctacagg tttggtaaca
    13441 gcgacagcag aaggtgaccc agttttagca attgccggac aagtaaaacg aagcgatcta
    13501 ttgaagttga cccatcaaag catggataat gcagcactgt tccagccaat cacaaaatac
    13561 agtgcagaaa tccaagatcc agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg
    13621 gctaagagtt caaaaaaagg cgcgagcttt atcagtatcc cgcaagatgt agtggatgca
    13681 cctgtccaag ggaatgtcat caagccattg agcgatccta aattaggttc tgcttctgca
    13741 gatgatatcc gttacctagc tgaacgcatt agagaagcaa aactaccggt actattagta
    13801 ggtatgcgag gatcaagtga aaaagaaacg ttagccatca ggcaattagt tggaaagaca
    13861 gcattgcctg ttgtagagac attccaagca gctggagtga tctcacgtga attagaagcg
    13921 catttctttg gtcgtgtggg tttatttaga aaccaaccag gagatatgct tttgaaacga
    13981 agtgatctag ttattgctat tggctatgat ccaatcgaat atgaagcaag aaactggaat
    14041 gcggaaaaag atgcgcggat cattgtgatc gatgaagcgc cagctgaaat cgatcctttt
    14101 atgcagcctg aaagagaatt gatcggtgat atctcagcca ctcttgattt attgacaggt
    14161 tcattagagc ctcaacaagt gtcagaagat gcaaaagaat atttagcatc tcttcaagca
    14221 gaattaacgg agcgggacat tgttcaatca aaaggagaag caggtatctt gcatccttta
    14281 gaagttatta acacgcttca gtcaaaagta acagatgata tgactgttac cgtagatgta
    14341 ggtagtcatt atatttggat ggcacgccac tttagaagtt atgaacctcg tcatttgtta
    14401 ttcagtaatg gtatgcaaac gcttggtgtt gcattgccat gggcgatttc tgctgcattg
    14461 gtgcgtccaa atacacaaat cgtatcagtt tccggcgatg ggggattttt attctctgcc
    14521 caggatttag aaacggcggt acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat
    14581 ggacactaca atatggtaga gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta
    14641 gactttggtc cagttgattt tgtgaaatat gcagaagctt ttggcgctaa aggaatccga
    14701 gctacaagtg tagaggaatt agaaaaagct ttagaagaag gatttgcgac agaaggaccc
    14761 gtaattattg atattccgat tgattaccgt gataatgaga aattgggaga aacaatctta
    14821 ccagatcaat tctattaagg aagtgtcgac atgactgaaa aaatcttata ccagcacggg
    14881 acattaggag ctttgatggc tggcttgatg gatggaacag aaacgattgc tcatatatta
    14941 gaaaaaggaa ctctgggatt gggaacattg catgggcttg atggtgaagt catcttttta
    15001 gatggcgtag cttatcaagg aagatcagat ggctcagttg cccaattaga tggaagtgaa
    15061 cttaccccct atgcagcaat tacagatttt acgccagata ctagtttttc tgtctcaaaa
    15121 actgcagatg gagaacaatt gaaaaaagac atattagtaa gagaagccgg ggaaaaccta
    15181 tttttggcag tgaagataac tggattattc aaaaatatgc acatccgtat catgccgaag
    15241 caagaaaaac cttacagaag actagcaaaa atttcagagt ctcaaccaga attccaacaa
    15301 agcaatgtcc aaggaacatt agtcgggttc tttacgcctg aattattcca aggagttgct
    15361 gcagcgggat ttcacttgca ttttatcgat gatacacata catttggcgg acatgtaatg
    15421 gattttgaag tggctgaagg aaaagttgag attagccgca tctcgtccct tgtacagcat
    15481 tttccagttg atgatccgac ttttgttcac gaaacgattg attatgctga tttagcagca
    15541 gaaatccaac aagcagaata gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac
    15601 gtatctcgtt tttttgagga ggaaacctag cgtttacaaa aaaactgagc tataatcttc
    15661 tccatagatc aaaaaggagc ggtttttatc atgtggacag aaaaacagaa aaaagatgca
    15721 tacgaaattt tattattaca gcaacaagga ttattagaag cagaagataa ctggttagca
    15781 aatttagcaa attcatctgc attgttgaat gagactttgc ctgagactgt ctttgcagga
    15841 tattatttat tcgatggtaa tgaattgatt ttaggaccgt tccaagggcg agtttcttgc
    15901 acacgtatca agatgggaaa aggagtttgt ggagaatcag ctgaaaaaca agaaacattg
    15961 atcgttgatg atgtcaaaac ccatgcaaat tacatttcct gcgattcagc cgcacaatca
    16021 gaaatcgtag tgccaatggt aaaagaaggt aatttaatcg gtgttttaga tatcgattgc
    16081 ggagtaacga aagggtatga tgaaatcgat caagcatttc tagaaaaata tgtagatctt
    16141 ttattagcaa attttaatat aaatgctttt tcatttatta ataactaaaa tcgtctattg
    16201 aaacaaaaaa gaagagaaat caagaataaa aagagccgga aagacagtgg gcatttctgt
    16261 ttttcggttt ttttattttt ttataaaatc acaagtgtta ttgattttca taatcatatt
    16321 ttaatggtat tatcatttta aataatggaa aaacattaag gatggatgaa atatgaataa
    16381 cgaaactatc attttaggaa aaaaatatat ttgccaacct attggcttga aacaaaaagt
    16441 ggttggagaa gtaatcagta aattagaaaa ttgtgttgtg atctgcgtag acgaatatca
    16501 tgacgtcgat cgcaatgaaa tattagaaaa atgcggaaaa gtagtggcaa aatatactca
    16561 cgtttatgag ttggaaacaa cagaatatct atataactat agcaacaaat atgaacatac
    16621 tttagtttca taaagattta aataaagtgc cttcaaaagc gagaagacca agcttttgaa
    16681 ggcactttat ttagttggca gtgattgttc atgatttttg atttattcaa aaggttcgcc
    16741 tagagaatct gtctctgtaa cgaggcctgg tggagaaact cgataaaaac ctatactgcc
    16801 agaactgtcc tattttcgga tagacaaact agtagtttta aaaagatcat ccgatccaat
    16861 catttcaaaa aatgcaaaac tgatatcagg atcatttagc tcaggcttgt tatcgtaaag
    16921 catttgtaaa ggttgcattt agttaggagt aaacgcgaaa ctatcatcat tatttgctga
    16981 agaagatgaa gaagctaatt cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc
    17041 tttaccctgt tttttgcaga ggtagaaact tttatatcgg aagtttctac cggagaattt
    17101 ttctcatttg tagaaattgt tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg
    17161 ttctttgatg cagaagtccc acaagcagta agtaacaata aatttgataa actgataaac
    17221 agaattcttt tcattatgta cacactcctt atagtatcgt ttagcataca cctatgctca
    17281 gtattaggca aaatcaatgc aaaactatca agtcttttat agtatctatt agtcttagta
    17341 atacatatat ggagagggtg atatcccagt tcaaaccaga tacattggtt accatctgta
    17401 ttaacgatta tgcctctaaa cgcttttttt atgaaaaaat ttataagtga aaacgtaggt
    17461 atgatattat ctagaggtaa tttttttggg tatttaaata gttgctagga ggatag
//
LOCUS       DAFELO010000055        17106 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFELO010000055
VERSION     DAFELO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.057"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            7..1398
                     /locus_tag="LP452_002052"
     CDS             7..1398
                     /locus_tag="LP452_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL2014755.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="LP452_002053"
     CDS             1414..2454
                     /locus_tag="LP452_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2014756.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="LP452_002054"
     CDS             2473..3558
                     /locus_tag="LP452_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL2014757.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="LP452_002055"
     CDS             3591..4553
                     /locus_tag="LP452_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2014758.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPQKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="LP452_002056"
     CDS             4546..5979
                     /locus_tag="LP452_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL2014759.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="LP452_002057"
     CDS             5976..7046
                     /locus_tag="LP452_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014760.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="LP452_002058"
     CDS             7033..8454
                     /locus_tag="LP452_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL2014761.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="LP452_002059"
     CDS             8467..9474
                     /locus_tag="LP452_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2014762.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="LP452_002060"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="LP452_002060"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBL2014763.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="LP452_002061"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="LP452_002061"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL2014764.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="LP452_002062"
     CDS             11608..12285
                     /locus_tag="LP452_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL2014765.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="LP452_002063"
     CDS             12275..13417
                     /locus_tag="LP452_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL2014766.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="LP452_002064"
     CDS             13430..14407
                     /locus_tag="LP452_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2014767.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="LP452_002065"
     CDS             14407..15315
                     /locus_tag="LP452_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBL2014768.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="LP452_002066"
     CDS             15316..16068
                     /locus_tag="LP452_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL2014769.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="LP452_002067"
     CDS             16073..17020
                     /locus_tag="LP452_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL2014770.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2189 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc aaaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gataga
//
LOCUS       DAFELO010000056        17006 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFELO010000056
VERSION     DAFELO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.058"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..127)
                     /locus_tag="LP452_002068"
     CDS             complement(<1..127)
                     /locus_tag="LP452_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL2014771.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="LP452_002069"
     CDS             complement(231..542)
                     /locus_tag="LP452_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2014772.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="LP452_002070"
     CDS             complement(544..741)
                     /locus_tag="LP452_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL2014773.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="LP452_002071"
     CDS             complement(1135..2862)
                     /locus_tag="LP452_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL2014774.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="LP452_002072"
     CDS             complement(2855..4048)
                     /locus_tag="LP452_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2014775.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="LP452_002073"
     CDS             complement(4235..4879)
                     /locus_tag="LP452_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2014776.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="LP452_002074"
     CDS             complement(4973..5107)
                     /locus_tag="LP452_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL2014777.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="LP452_002075"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="LP452_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL2014778.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="LP452_002076"
     CDS             complement(7238..7711)
                     /locus_tag="LP452_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL2014779.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="LP452_002077"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="LP452_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL2014780.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="LP452_002078"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="LP452_002078"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL2014781.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="LP452_002079"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="LP452_002079"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL2014782.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="LP452_002080"
     CDS             complement(11758..12897)
                     /locus_tag="LP452_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2014783.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="LP452_002081"
     CDS             complement(13206..13919)
                     /locus_tag="LP452_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL2014784.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="LP452_002082"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="LP452_002082"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL2014785.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="LP452_002083"
     CDS             15108..16640
                     /locus_tag="LP452_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2014786.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17006)
                     /locus_tag="LP452_002084"
     CDS             complement(16831..>17006)
                     /locus_tag="LP452_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2014787.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
BASE COUNT         5279 a         3493 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gaaaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaata
//
LOCUS       DAFELO010000057        16843 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFELO010000057
VERSION     DAFELO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.059"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            163..1104
                     /locus_tag="LP452_002085"
     CDS             163..1104
                     /locus_tag="LP452_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL2014788.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="LP452_002086"
     CDS             1173..1904
                     /locus_tag="LP452_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL2014789.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="LP452_002087"
     CDS             2048..4228
                     /locus_tag="LP452_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL2014790.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="LP452_002088"
     CDS             4248..4730
                     /locus_tag="LP452_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL2014791.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="LP452_002089"
     CDS             4746..5507
                     /locus_tag="LP452_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL2014792.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="LP452_002090"
     CDS             5638..6972
                     /locus_tag="LP452_002090"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL2014793.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="LP452_002091"
     CDS             7279..7764
                     /locus_tag="LP452_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL2014794.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7757..8440
                     /locus_tag="LP452_002092"
     CDS             7757..8440
                     /locus_tag="LP452_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL2014795.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8566..11211
                     /gene="polA"
                     /locus_tag="LP452_002093"
     CDS             8566..11211
                     /gene="polA"
                     /locus_tag="LP452_002093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL2014796.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11256..12092
                     /gene="mutM"
                     /locus_tag="LP452_002094"
     CDS             11256..12092
                     /gene="mutM"
                     /locus_tag="LP452_002094"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL2014797.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12056..12685
                     /locus_tag="LP452_002095"
     CDS             12056..12685
                     /locus_tag="LP452_002095"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL2014798.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12737..13069
                     /locus_tag="LP452_002096"
     CDS             12737..13069
                     /locus_tag="LP452_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL2014799.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13381..13872
                     /gene="nrdR"
                     /locus_tag="LP452_002097"
     CDS             13381..13872
                     /gene="nrdR"
                     /locus_tag="LP452_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL2014800.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13895..15268
                     /locus_tag="LP452_002098"
     CDS             13895..15268
                     /locus_tag="LP452_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL2014801.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15268..16194
                     /gene="dnaI"
                     /locus_tag="LP452_002099"
     CDS             15268..16194
                     /gene="dnaI"
                     /locus_tag="LP452_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL2014802.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16323..16763
                     /locus_tag="LP452_002100"
     CDS             16323..16763
                     /locus_tag="LP452_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL2014803.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5748 a         2949 c         3710 g         4436 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     8521 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     8581 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     8641 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     8701 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     8761 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     8821 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     8881 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     8941 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     9001 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     9061 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     9121 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     9181 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     9241 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     9301 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     9361 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     9421 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     9481 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     9541 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     9601 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     9661 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     9721 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     9781 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     9841 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     9901 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     9961 ttgagccaag aactggcaga taaaaaccaa gaagatcttt tccggaaaat ggaactgcca
    10021 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
    10081 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
    10141 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
    10201 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
    10261 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
    10321 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
    10381 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
    10441 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
    10501 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
    10561 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
    10621 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
    10681 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
    10741 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
    10801 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
    10861 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
    10921 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
    10981 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
    11041 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
    11101 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
    11161 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
    11221 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
    11281 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
    11341 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
    11401 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
    11461 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
    11521 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
    11581 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
    11641 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
    11701 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
    11761 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
    11821 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
    11881 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
    11941 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
    12001 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
    12061 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
    12121 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
    12181 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
    12241 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
    12301 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
    12361 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
    12421 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
    12481 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
    12541 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
    12601 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
    12661 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
    12721 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
    12781 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
    12841 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
    12901 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
    12961 acctgatcgt agtaaaacac cgctcccaga aagtgaccac atttccgatg agcaaaaaat
    13021 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
    13081 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
    13141 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
    13201 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
    13261 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
    13321 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
    13381 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
    13441 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
    13501 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
    13561 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
    13621 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
    13681 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
    13741 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
    13801 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
    13861 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
    13921 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
    13981 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
    14041 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
    14101 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
    14161 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
    14221 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
    14281 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
    14341 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
    14401 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
    14461 atctgaatca gtagcattac ttgaagcgga ttcgacattc gattggcagt ttttgaatga
    14521 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
    14581 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
    14641 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
    14701 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
    14761 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
    14821 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
    14881 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
    14941 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
    15001 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
    15061 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
    15121 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
    15181 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
    15241 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
    15301 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
    15361 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
    15421 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
    15481 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
    15541 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
    15601 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
    15661 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
    15721 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
    15781 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
    15841 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
    15901 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
    15961 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
    16021 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
    16081 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
    16141 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
    16201 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
    16261 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
    16321 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
    16381 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
    16441 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatta
    16501 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
    16561 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
    16621 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
    16681 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
    16741 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
    16801 tatagaacga aaaggtatat tttttaagga gatatgggaa ttt
//
LOCUS       DAFELO010000058        16522 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFELO010000058
VERSION     DAFELO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.060"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(142..417)
                     /locus_tag="LP452_002101"
     CDS             complement(142..417)
                     /locus_tag="LP452_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL2014804.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /gene="der"
                     /locus_tag="LP452_002102"
     CDS             complement(660..1970)
                     /gene="der"
                     /locus_tag="LP452_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL2014805.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="LP452_002103"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="LP452_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL2014806.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="LP452_002104"
     CDS             complement(3516..4196)
                     /locus_tag="LP452_002104"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL2014807.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="LP452_002105"
     CDS             complement(4245..4880)
                     /locus_tag="LP452_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2014808.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="LP452_002106"
     CDS             complement(4946..6349)
                     /locus_tag="LP452_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL2014809.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="LP452_002107"
     CDS             complement(6336..7367)
                     /locus_tag="LP452_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014810.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="LP452_002108"
     CDS             7398..7619
                     /locus_tag="LP452_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL2014811.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="LP452_002109"
     CDS             complement(7784..8521)
                     /locus_tag="LP452_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL2014812.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="LP452_002110"
     CDS             complement(8674..10014)
                     /locus_tag="LP452_002110"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL2014813.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="LP452_002111"
     CDS             complement(10251..11774)
                     /locus_tag="LP452_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL2014814.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="LP452_002112"
     CDS             complement(12152..12763)
                     /locus_tag="LP452_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2014815.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="LP452_002113"
     CDS             complement(13093..13809)
                     /locus_tag="LP452_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL2014816.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="LP452_002114"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="LP452_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL2014817.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="LP452_002115"
     CDS             complement(14368..15165)
                     /locus_tag="LP452_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL2014818.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKGEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="LP452_002116"
     CDS             complement(15135..15506)
                     /locus_tag="LP452_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL2014819.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="LP452_002117"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="LP452_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL2014820.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3576 c         2798 g         5503 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacttgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacctttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tt
//
LOCUS       DAFELO010000059        16509 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFELO010000059
VERSION     DAFELO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.061"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            184..1182
                     /locus_tag="LP452_002118"
     CDS             184..1182
                     /locus_tag="LP452_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2014821.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="LP452_002119"
     CDS             1294..1803
                     /locus_tag="LP452_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL2014822.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="LP452_002120"
     CDS             complement(1833..2492)
                     /locus_tag="LP452_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL2014823.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="LP452_002121"
     CDS             complement(2494..3258)
                     /locus_tag="LP452_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL2014824.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="LP452_002122"
     CDS             complement(3266..3943)
                     /locus_tag="LP452_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL2014825.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="LP452_002123"
     CDS             complement(3980..5365)
                     /locus_tag="LP452_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2014826.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="LP452_002124"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LP452_002124"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LP452_002125"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LP452_002125"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL2014827.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="LP452_002126"
     CDS             6304..7161
                     /locus_tag="LP452_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014828.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="LP452_002127"
     CDS             complement(7242..7577)
                     /locus_tag="LP452_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014829.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="LP452_002128"
     CDS             complement(7644..9014)
                     /locus_tag="LP452_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL2014830.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="LP452_002129"
     CDS             complement(9092..9628)
                     /locus_tag="LP452_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2014831.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="LP452_002130"
     CDS             9937..11418
                     /locus_tag="LP452_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2014832.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11521..12228)
                     /locus_tag="LP452_002131"
     CDS             complement(11521..12228)
                     /locus_tag="LP452_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL2014833.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12307..12645)
                     /locus_tag="LP452_002132"
     CDS             complement(12307..12645)
                     /locus_tag="LP452_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL2014834.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12756..13718)
                     /locus_tag="LP452_002133"
     CDS             complement(12756..13718)
                     /locus_tag="LP452_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL2014835.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLVSGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13733..15064)
                     /locus_tag="LP452_002134"
     CDS             complement(13733..15064)
                     /locus_tag="LP452_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2014836.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15072..15752)
                     /locus_tag="LP452_002135"
     CDS             complement(15072..15752)
                     /locus_tag="LP452_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL2014837.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15971..16450)
                     /locus_tag="LP452_002136"
     CDS             complement(15971..16450)
                     /locus_tag="LP452_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL2014838.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         4885 a         3441 c         2736 g         5447 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat aaatggtatt
    11461 caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa cttctttatt
    11521 ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca ggaatgtcga
    11581 aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg ccatcggtac
    11641 ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc gagacgcgtt
    11701 aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag cgaaaatccc
    11761 gatattgatt aacgtagagt tccagctaag caatacacct accataatca aggggaacga
    11821 aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca atgggacata
    11881 acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta tcgcagcaac
    11941 tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg aattgtagtt
    12001 atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt gactttgtaa
    12061 tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt gatcgtattt
    12121 tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc caagaatgac
    12181 caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt tcgtttcact
    12241 ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa agaccttgaa
    12301 tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa atccttctac
    12361 cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt ttgttccttg
    12421 tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca cgctatcgcc
    12481 ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta tgacagtttc
    12541 ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc ctcgatcttc
    12601 atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct ttctccttta
    12661 aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat acatttgtga
    12721 tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct gctcttatac
    12781 gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa atcttttgca
    12841 ttcgcaccac ttactaatgg atggcctcct ccatgatggc gtttagccaa ttcattgatg
    12901 atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg aacaaaaatc
    12961 ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc tgtctctgaa
    13021 tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc tgcaccattt
    13081 tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt catcggcatt
    13141 tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa tatcgaagca
    13201 atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc tccgacgatt
    13261 cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg aaacatcaaa
    13321 gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca cacaagatct
    13381 ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc caactgatat
    13441 ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag cgctccttgg
    13501 tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc aatcgtttca
    13561 cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc taatcctact
    13621 tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat tttatcgtat
    13681 ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct ttctaagaac
    13741 gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca ataaacactt
    13801 cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga atgtctaatt
    13861 cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc aagatatttc
    13921 gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg ctaagtacgc
    13981 caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat ttgaacttag
    14041 ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt ccagatatct
    14101 gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc gctttcatta
    14161 catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc ggcatgactt
    14221 ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt ttctttacac
    14281 tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc cctaaaacat
    14341 cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga aaacgcgaat
    14401 gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc tgcaaataat
    14461 gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt ttgatcaatt
    14521 ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa gaggtgcgta
    14581 agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta ttgaatcctc
    14641 ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc gcccggttgc
    14701 cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta gtcatcgcgc
    14761 cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa acgtcccctt
    14821 caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt aatttgcgtt
    14881 cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt tctgcatctt
    14941 tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg cttcttacag
    15001 aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca tgttttgtcg
    15061 ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc ttcactatct
    15121 tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt attgtaatgg
    15181 attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc ttctggcccc
    15241 atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc ttctgcaagc
    15301 aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt tccttctgct
    15361 tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac ttcatagctt
    15421 gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg ttttccaccg
    15481 atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat ctcaacaata
    15541 ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat gtggtcacta
    15601 tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa gtctgtcaat
    15661 ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat ttcaattgtt
    15721 aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt tctctgtatc
    15781 ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag ctgctaaata
    15841 tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat aatacaggat
    15901 gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc ttagcttctc
    15961 ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt tctaaatcgg
    16021 ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact tcttcttttt
    16081 cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt attccaatag
    16141 gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt ccaggaaaac
    16201 caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga tcgttcaaag
    16261 gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt ttacatatca
    16321 aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct accacatgac
    16381 taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg acttttgttt
    16441 gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag ggatgctgta
    16501 ttcaaaaat
//
LOCUS       DAFELO010000060        16402 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFELO010000060
VERSION     DAFELO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.062"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            374..3181
                     /locus_tag="LP452_002137"
     CDS             374..3181
                     /locus_tag="LP452_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2014839.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3372..3863
                     /locus_tag="LP452_002138"
     CDS             3372..3863
                     /locus_tag="LP452_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2014840.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3966..4955
                     /gene="manX"
                     /locus_tag="LP452_002139"
     CDS             3966..4955
                     /gene="manX"
                     /locus_tag="LP452_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL2014841.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4988..5791
                     /locus_tag="LP452_002140"
     CDS             4988..5791
                     /locus_tag="LP452_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL2014842.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5812..6726
                     /locus_tag="LP452_002141"
     CDS             5812..6726
                     /locus_tag="LP452_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL2014843.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6809..7183
                     /locus_tag="LP452_002142"
     CDS             6809..7183
                     /locus_tag="LP452_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL2014844.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7322..7567
                     /locus_tag="LP452_002143"
     CDS             7322..7567
                     /locus_tag="LP452_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2014845.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7739..8803
                     /locus_tag="LP452_002144"
     CDS             7739..8803
                     /locus_tag="LP452_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL2014846.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9049..9474
                     /locus_tag="LP452_002145"
     CDS             9049..9474
                     /locus_tag="LP452_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2014847.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9467..9916
                     /gene="nusB"
                     /locus_tag="LP452_002146"
     CDS             9467..9916
                     /gene="nusB"
                     /locus_tag="LP452_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL2014848.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10013..10867
                     /locus_tag="LP452_002147"
     CDS             10013..10867
                     /locus_tag="LP452_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL2014849.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10868..12205
                     /locus_tag="LP452_002148"
     CDS             10868..12205
                     /locus_tag="LP452_002148"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL2014850.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12210..12434
                     /locus_tag="LP452_002149"
     CDS             12210..12434
                     /locus_tag="LP452_002149"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL2014851.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12436..13326
                     /locus_tag="LP452_002150"
     CDS             12436..13326
                     /locus_tag="LP452_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL2014852.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13335..14153
                     /locus_tag="LP452_002151"
     CDS             13335..14153
                     /locus_tag="LP452_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL2014853.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14260..14709
                     /locus_tag="LP452_002152"
     CDS             14260..14709
                     /locus_tag="LP452_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL2014854.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14727..>16402
                     /gene="recN"
                     /locus_tag="LP452_002153"
     CDS             14727..>16402
                     /gene="recN"
                     /locus_tag="LP452_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL2014855.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAK"
BASE COUNT         5429 a         2716 c         3691 g         4566 t
ORIGIN      
        1 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
       61 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
      121 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
      181 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
      241 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
      301 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
      361 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
      421 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
      481 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
      541 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
      601 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
      661 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
      721 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
      781 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
      841 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
      901 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
      961 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     1021 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     1081 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     1141 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     1201 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     1261 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     1321 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     1381 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     1441 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     1501 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     1561 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     1621 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     1681 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     1741 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     1801 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     1861 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     1921 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     1981 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     2041 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     2101 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     2161 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     2221 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     2281 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     2341 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     2401 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     2461 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     2521 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     2581 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     2641 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     2701 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     2761 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     2821 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     2881 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     2941 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     3001 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     3061 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     3121 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     3181 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     3241 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     3301 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     3361 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     3421 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     3481 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     3541 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     3601 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     3661 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     3721 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     3781 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     3841 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     3901 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     3961 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     4021 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     4081 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     4141 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     4201 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     4261 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     4321 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     4381 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     4441 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     4501 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     4561 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     4621 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     4681 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     4741 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     4801 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     4861 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     4921 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     4981 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     5041 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     5101 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     5161 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     5221 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     5281 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     5341 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     5401 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     5461 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     5521 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     5581 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     5641 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     5701 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     5761 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     5821 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     5881 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     5941 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     6001 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     6061 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     6121 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     6181 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     6241 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     6301 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     6361 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     6421 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     6481 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     6541 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     6601 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     6661 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     6721 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     6781 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     6841 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     6901 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     6961 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     7021 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     7081 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     7141 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
     7201 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
     7261 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
     7321 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
     7381 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
     7441 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
     7501 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
     7561 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
     7621 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
     7681 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
     7741 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
     7801 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
     7861 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
     7921 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
     7981 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
     8041 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
     8101 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
     8161 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
     8221 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
     8281 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
     8341 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
     8401 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
     8461 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
     8521 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
     8581 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
     8641 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
     8701 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
     8761 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
     8821 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
     8881 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
     8941 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
     9001 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
     9061 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
     9121 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
     9181 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
     9241 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
     9301 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
     9361 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
     9421 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
     9481 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
     9541 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
     9601 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
     9661 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
     9721 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
     9781 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
     9841 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt
     9901 agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc
     9961 gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga
    10021 attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca
    10081 aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc
    10141 agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc
    10201 attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa
    10261 atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat
    10321 agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc
    10381 gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg
    10441 catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat
    10501 cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac
    10561 tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat
    10621 tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc
    10681 tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa
    10741 atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc
    10801 aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg
    10861 tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa
    10921 atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg
    10981 tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc
    11041 tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt
    11101 ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga
    11161 gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa
    11221 gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg
    11281 aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa
    11341 aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc
    11401 agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat
    11461 gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt
    11521 tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga
    11581 taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga
    11641 attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt
    11701 gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg
    11761 ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca
    11821 gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac
    11881 gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg
    11941 ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca
    12001 acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat
    12061 catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga
    12121 gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt
    12181 acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc
    12241 aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc
    12301 tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag
    12361 cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa
    12421 acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa
    12481 aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg
    12541 agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt
    12601 gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg
    12661 gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt
    12721 agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat
    12781 ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag
    12841 cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa
    12901 gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat
    12961 gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag
    13021 caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt
    13081 cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct
    13141 ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca
    13201 cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag
    13261 gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag
    13321 aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat
    13381 ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa
    13441 acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag
    13501 gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat
    13561 ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta
    13621 ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc
    13681 agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc
    13741 gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt
    13801 cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag
    13861 aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg
    13921 gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg
    13981 cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa
    14041 atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta
    14101 ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa
    14161 ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta
    14221 tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat
    14281 cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa
    14341 atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa
    14401 ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa
    14461 acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt
    14521 gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca
    14581 aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat
    14641 gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat
    14701 tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga
    14761 ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg
    14821 gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa
    14881 gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa
    14941 aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc
    15001 tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata
    15061 ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa
    15121 acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag
    15181 atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc
    15241 tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata
    15301 tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga
    15361 aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg
    15421 caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg
    15481 tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata
    15541 tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt
    15601 tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac
    15661 agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca
    15721 ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac
    15781 tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa
    15841 aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa
    15901 acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat
    15961 ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag cctttggtga
    16021 aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt
    16081 ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag
    16141 ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca
    16201 tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag
    16261 tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag
    16321 aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag
    16381 aactattgaa actcgcaaaa ag
//
LOCUS       DAFELO010000061        15853 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFELO010000061
VERSION     DAFELO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.063"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            293..1432
                     /locus_tag="LP452_002154"
     CDS             293..1432
                     /locus_tag="LP452_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL2014856.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1499..2074)
                     /locus_tag="LP452_002155"
     CDS             complement(1499..2074)
                     /locus_tag="LP452_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2014857.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2079..3053)
                     /locus_tag="LP452_002156"
     CDS             complement(2079..3053)
                     /locus_tag="LP452_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL2014858.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3077..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3336..4304
                     /locus_tag="LP452_002157"
     CDS             3336..4304
                     /locus_tag="LP452_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2014859.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4417..4695
                     /locus_tag="LP452_002158"
     CDS             4417..4695
                     /locus_tag="LP452_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL2014860.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4813..5049
                     /gene="secG"
                     /locus_tag="LP452_002159"
     CDS             4813..5049
                     /gene="secG"
                     /locus_tag="LP452_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL2014861.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5152..5907
                     /locus_tag="LP452_002160"
     CDS             5152..5907
                     /locus_tag="LP452_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2014862.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5910..8267
                     /gene="rnr"
                     /locus_tag="LP452_002161"
     CDS             5910..8267
                     /gene="rnr"
                     /locus_tag="LP452_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL2014863.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8289..8753
                     /gene="smpB"
                     /locus_tag="LP452_002162"
     CDS             8289..8753
                     /gene="smpB"
                     /locus_tag="LP452_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL2014864.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9055..9774
                     /gene="atpB"
                     /locus_tag="LP452_002163"
     CDS             9055..9774
                     /gene="atpB"
                     /locus_tag="LP452_002163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL2014865.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9827..10042
                     /gene="atpE"
                     /locus_tag="LP452_002164"
     CDS             9827..10042
                     /gene="atpE"
                     /locus_tag="LP452_002164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL2014866.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10133..10657
                     /gene="atpF"
                     /locus_tag="LP452_002165"
     CDS             10133..10657
                     /gene="atpF"
                     /locus_tag="LP452_002165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL2014867.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10644..11186
                     /locus_tag="LP452_002166"
     CDS             10644..11186
                     /locus_tag="LP452_002166"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL2014868.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11209..12765
                     /locus_tag="LP452_002167"
     CDS             11209..12765
                     /locus_tag="LP452_002167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL2014869.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12779..13681
                     /locus_tag="LP452_002168"
     CDS             12779..13681
                     /locus_tag="LP452_002168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL2014870.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13704..15110
                     /gene="atpD"
                     /locus_tag="LP452_002169"
     CDS             13704..15110
                     /gene="atpD"
                     /locus_tag="LP452_002169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL2014871.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15124..15546
                     /locus_tag="LP452_002170"
     CDS             15124..15546
                     /locus_tag="LP452_002170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL2014872.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5264 a         2840 c         3419 g         4330 t
ORIGIN      
        1 ttttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
       61 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
      121 agtagaatag gaatctatga gcaagtcctc tcgtataaat aaaataggag ggaagtagca
      181 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
      241 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
      301 agaagaaaaa atcgcgattt tacaagagat catccgaatt aaatcagtga atggaaacga
      361 aggtgaagta gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat
      421 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
      481 aaaagtgtta ggcctatctg ggcatatgga tgtcgtggcg gctggtgatg aatcgtcttg
      541 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
      601 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
      661 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
      721 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
      781 agagccaact aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
      841 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
      901 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
      961 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
     1021 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
     1081 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
     1141 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
     1201 tcctttgatt cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc
     1261 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
     1321 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
     1381 tttggatatg attgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
     1441 taataaatga aaaaacagag ccagattagc attattttgc taatccagct ctgtttattc
     1501 agcctatttt tctttttgaa tatatcaagc gttgtggcaa catagtgatg actaatgaaa
     1561 caacgacgat agacaacaaa cgatcaaggt agtctgtacc tgcctgtacc aagatcacac
     1621 ttgttgtctg acttaaacct aacccatgta atagctgaac gatcatactg gatcctgagg
     1681 aggtgatccc gccaaataac tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg
     1741 acaaaatcaa agcgagtaat aacagtttca agctgttttt atctttgccg agatatcttg
     1801 gcaaaatcaa tccagcaagc aagcctgtga gaatttggat tggcgaataa tacagagaaa
     1861 aaatatctgt agtcactcca cttaaaatac cgctggacaa tcctgtcagc attccaggta
     1921 caggcccaag caatgcacca gcaaaaatcg ttccgataga atcgagatag attggcaaac
     1981 gaagccataa agcaatcgtt cctccaatat aattgatccc aatacacaaa gccatcactg
     2041 taagtacgcg agttgccatt ttgtttttat tcattttgtt agcccatctt taacctttcc
     2101 aaatctttca taatcgtttc ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa
     2161 aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg ccaaaattca
     2221 taacgatcaa ccaatgtttg tccacgactt attccttgag tttccacagc agtgaagctt
     2281 ttgaaacctt cacagatggc cccctcgata aaataagcaa cagctaacgg atcattgatg
     2341 acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg agtgatcgct
     2401 tttagatact ctccctcttc aggattcatt tgacagcagt attcgaggat agtcggtgtc
     2461 aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt gagattctcg
     2521 aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac tggcgaacag
     2581 ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg tttcccaata
     2641 tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc aatgactgat
     2701 gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag aggttctgcc
     2761 tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc atgagtgtct
     2821 tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac aggaatgtct
     2881 aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc tgcagggaca
     2941 ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac agctaatgat
     3001 aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg cattatttcc
     3061 aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga accagtccaa
     3121 gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg cgaatgtaaa
     3181 tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca atgaaaaaga
     3241 aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt ggataatagt
     3301 tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat ttcattctta
     3361 ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca aagaaattag
     3421 taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat cgtttgatgg
     3481 accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa gagtgtgaaa
     3541 gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg aaactagctg
     3601 gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta catgattacc
     3661 gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag aaaggattca
     3721 acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc atcggaatgg
     3781 gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat gaacagccga
     3841 atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg atggcaagtg
     3901 gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat gtttctgttt
     3961 atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa atggtcatga
     4021 gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa gcatcagcta
     4081 cgagacagct tggcagcaac catctgcctt tattggtcat tcatggtgaa agagatcatt
     4141 ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat aaagcactgc
     4201 tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa acctattgga
     4261 cagtgatttt ttcatttatt gaacaaagaa taacgatgaa ttaattttct cttctttttt
     4321 gtaagtaatt cgaggataaa taattgttta tagatcagga gactgctaaa ataaggtagg
     4381 aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga agaaaaaaat
     4441 cgtcttgctt tattcaatga tgaacaacaa gagatcggag aaatgacatg gtcagatgcg
     4501 ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg tggtcaaaag
     4561 ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg gaaaaagatc
     4621 attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata tcaagacgtc
     4681 ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt attccattta
     4741 tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat cttttgggaa
     4801 ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc gattgttatg
     4861 gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc atttaccggc
     4921 ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt catgcagcga
     4981 tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc atatttatct
     5041 tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat tgcaaagaga
     5101 acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga ggtgagacga
     5161 gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt tttgctgctt
     5221 cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt ggaaaaagca
     5281 gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc atatgatatt
     5341 ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca ttttttacag
     5401 tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat ctttgctgta
     5461 cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc tttgtctcct
     5521 gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct gaagacagca
     5581 ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt agctgaacaa
     5641 caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc agatatccaa
     5701 cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa tggcgtattt
     5761 gaaactgcac gcaagttgag ccgtcagcga gtgacacttc gatggtatcc tgaaagtggt
     5821 catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt ggaattttta
     5881 gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa gagaaaattc
     5941 tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc gctgaaggtt
     6001 tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct caaatggaaa
     6061 gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta aaaccaatct
     6121 tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg attgatcccg
     6181 aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat ggagacaccg
     6241 tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca gaggggaaag
     6301 tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt gcttattcag
     6361 aagaagaaat acaagaaacg gatctttacg gcgttgttat cccgaaagat agaaagctca
     6421 accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga agtattgtgc
     6481 tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa gggataatca
     6541 agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt gtcgtggcta
     6601 atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa gttccagatc
     6661 aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc attgttacga
     6721 ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa ttagataacg
     6781 gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca caaggcagtc
     6841 aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat cgagtgattc
     6901 cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat gttccaaggc
     6961 tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct catgatattt
     7021 tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat caaatcttag
     7081 aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg ttccaagaaa
     7141 tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg atttcttttg
     7201 aggatcgcga agcaaaaatc cttgttgatg ctgaaggaca cccaaatgat atccagctaa
     7261 gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc aatgaaacgg
     7321 tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat gaacagccaa
     7381 aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt ctggtccgag
     7441 gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga gtagaagaca
     7501 aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa gcacgctatt
     7561 cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac tttacatctc
     7621 ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac agtgaagata
     7681 aaagtgaaag caatcaaagt tcgtgggcta cggaacttcc tgaaattgca gatcatagtt
     7741 ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg aagaaagcag
     7801 aattcatgat ggacaaagtg ggggaagaat tcgatgggat catcagctct gttgtgaaat
     7861 tcggcctgtt tatcgaactg ccgaatacag tagagggatt gatccatatc aatgaattga
     7921 aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa agaactggtc
     7981 tgacttttaa aatcggacag aaagtgcgag taaaagtagt caaagctgat ccagatgaac
     8041 gagcagtaga ttttgaactt gtttccgcag aagaggttac agcgttggaa cggccaaaaa
     8101 cacgaaatgg aaaatcagga aaacgcgaag gaaaacaagc aggaaaacgc ccagaaagaa
     8161 atggcaagaa gaacaaacga tatagcgata gtaagcaaac gttcggtaag aaaaaaggga
     8221 agaagccatt ttacaaaggt gttaaaaaga agaaaaagaa aaaataaaat gagttggagg
     8281 aaacagctat gccaaaaggc gagggaaaat taattgcaca aaacaagaaa gctcgccatg
     8341 attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacc gagatcaagt
     8401 cgatacgaaa cagccggatc aatctaaaag atggatttat tcgcgtccgc aacggagaag
     8461 ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt aatcatgatc
     8521 cgttgcgcac gagaaagtta ttattacaca aaaaacaaat catccggctt gaaaatgaat
     8581 tgaaaaatac tggaatcact gttgttcctt taaaagtcta tattcgtaac ggctatgcca
     8641 aggtattgat tggtctggcg aaagggaaaa aatcttatga taaacgggaa gatttgaaac
     8701 gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga taatttcatt
     8761 atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt gttagcattt
     8821 aatctgataa aacatgatta tcataggttt tttcttagat caggagcacc gtgtataaag
     8881 aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg aaaatgtttt
     8941 gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg gaaaaaacca
     9001 gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa tggattggat
     9061 gaacgctcgc taacgttcca cattggacca atttggtttg atggtacagt ttgtataatg
     9121 gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag aaatcttaag
     9181 ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag tcgaggaatc
     9241 gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc atttacattg
     9301 tttttatttg tttttgttgc aaacaacatc ggtttagtaa caaaaatcgt tttacctaac
     9361 gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac tttggcattt
     9421 ataatgatct tacttactca cttatttggg gtgaaaaagt taggcttcaa aggatatttc
     9481 gtaaattcgt tcttgaaacc atatagtttt atgtttccga tgaaactgat tgaagaattt
     9541 acaaacttat taacacttgc tttacgtctt tatgggaata tttatgcggg ggaagttttg
     9601 ctaaccttga ttgcaaaaat ggtaataagc ttaggctggt tctcattgcc gttagcaatt
     9661 ccattagaga tggtctggat cgcattctcc ttgtttatcg gaagcatcca agcatttgtc
     9721 ttcgttactt tatctatggt ttatatgagc cataagattg aagtggaaga ataatattcg
     9781 ttttaaaaac aaactactat ttatctaatt taggaggaaa acaattatga attatatcgc
     9841 agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg gtcaagttat
     9901 ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat tccgtacaac
     9961 aatgtttatc ggggtagctc tagtcgaagc tgttcctatc ttaggggtag ttgtcgcctt
    10021 gattctagtt tttggtgttt aattgaatca gtattaaggc agaaacaatt tggtttgtta
    10081 ctgctttttt acatgaaaac tagaagtact tcaaagaaag gaaggctcgc ctatgctgaa
    10141 tcaattggca attgcagaag ttggcaatgc gatgttagga aatgtcatcg ttgtcagcgg
    10201 ctcatttttg atactactgg ctctcttgaa gcattttgct tggggaccaa tcagcgatat
    10261 tttgaaaaaa cgtgaagaca agatcgccaa tgatttggat tctgcagaac aatctcgcat
    10321 caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc gttctgatgc
    10381 agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa atattttgaa
    10441 ggatgctcaa gaagaagcag ctcgtctaaa aagtaaagcc caagctgata tcactgtaga
    10501 acgtgattca gcgctgaact ctgtaaaaga cgatgttgca gaactatctc ttcaaatcgc
    10561 ggaaaaaatc ttgaacaaag aattatctcc agaaatgcac gaatcattaa tcaatcaata
    10621 tatcgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga agacgttacg
    10681 ggaaagcttt atttgaactg gcgatcgatt caaataaagc agaagaaatt tatcaagatc
    10741 tcgtaagctt acgtcaaatc tatgaacaag tacctggatt aggtaatatg ttaagtgatg
    10801 ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgttagt ggctatgatg
    10861 gaatcgttaa aaacttttta gaagttgttt atgcgtataa ccgaatggat gatcttttgt
    10921 tcatgatcga agaatatgaa cgtcgatatg atgaacatca aggtttgctg ttgggaagtg
    10981 tcacaacagc agttccactt tcagacgaac aacgaataca attagaaaag aatgttgcaa
    11041 aaacaatgaa ctatcaatca gtcgaattaa aacaaatcgt tgattcttct attattggtg
    11101 gcgcgattgt tgaagctaac catcgagtaa tcgacggaag cattcgcacg caattagaaa
    11161 atatgcgtaa tcaattaaat agatagtaca aagaggtagg tgaatcgaat ggccatcaaa
    11221 gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa cgtgctttca
    11281 gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc acatggttta
    11341 gaaaacgcga tgagtggtga gttgcttgaa ttttcaaatg gctcatacgg aatggcgcaa
    11401 aacttagaat caaatgatgt aggtatcatt atccttggcg atttcgagtc cattcgtgaa
    11461 ggagataaag taaaacgaac aggaaagatc atggaagttc cagttggaga ggccttgatt
    11521 ggacgggtag ttaatccgct aggtcagcca attgacggac taggtgaaat cgttactgac
    11581 aaagctcgtc cagtagaagc aatggcacca ggcgttatgc aacgtaaatc tgttaacgaa
    11641 ccaatgcaaa caggtctaaa agcaatcgac gctcttgtac caatcggacg cggccaacgt
    11701 gaattagtta tcggtgaccg taaaacaggg aaaacttcca ttgcgatcga tacgatcatc
    11761 aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa agattctaca
    11821 gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac aatcgttgtg
    11881 aatgccggtg cgtctcaacc agcgccgttg ctttatatcg caccatatgc tggtactgca
    11941 atgggtgaag agttcatgta caacgggaaa catgtcttga tcatttttga tgatctttca
    12001 aaacaagctg ttgcttatcg tgaactttcc ttgctgcttc gccgtccacc aggtcgtgaa
    12061 gcttatccag gggatgtttt ctacttacac tcacgcttat tggaacgtgc agcaaaatta
    12121 agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac acaagctgga
    12181 gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca aatcttctta
    12241 gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact atctgtatct
    12301 cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac tttacgtcta
    12361 gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga cttggatgca
    12421 gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa acaaaaattg
    12481 catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac acatggattc
    12541 ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt cgaatatctg
    12601 gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct tccagaagaa
    12661 gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac aaaaggaaat
    12721 aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg tgagatgaat
    12781 gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa cgagccaaat
    12841 cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag cttcttcaca
    12901 gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga ctgctactca
    12961 gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca gcatgttgct
    13021 cagccgtcct gttaagaaaa cgggttatat cgtcatcact gcagatggcg gtttagtcgg
    13081 tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag accaccaatc
    13141 acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct ttaaagcacg
    13201 cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct ttgatgaagt
    13261 ccgcaaaatc gtaaatatgg caacaactat gtaccaaaac gaagtattcg atgaattata
    13321 cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag aaaaaatgct
    13381 gccaatctca gacttagatc cagaagaagc aactactttc gaacaagaat atatttttga
    13441 accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa gtttgatcta
    13501 cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag caatgaaaac
    13561 agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttaca accgtgcaag
    13621 acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg cactagaata
    13681 gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta atcggtcccg
    13741 ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac gcattagttg
    13801 tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta gaattagggg
    13861 acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga ggaatggaag
    13921 tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta ggtcgcgtat
    13981 tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat gcggaaagaa
    14041 gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca gaaattttgg
    14101 aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg aaagtcggac
    14161 tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc cataatatcg
    14221 cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact cgtgaaggta
    14281 atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc atggtgttcg
    14341 gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt ttgacgattg
    14401 cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac aacattttcc
    14461 gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct tctgcggttg
    14521 gttatcaacc aacattggca acagaaatgg gtcaattgca agaacgtatc acatctacga
    14581 aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac tacactgacc
    14641 cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa cgtaaattaa
    14701 cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt gctttagcac
    14761 ctgaaatcgt gggagaagaa cattataaag tagcaacaga agtccaacac gtattgcaac
    14821 gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta tccgatcaag
    14881 aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa aactttaacg
    14941 tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa acagtgaaag
    15001 gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct ttccgaagtg
    15061 ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag aaaggggtgc
    15121 cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt ctatgatcac
    15181 catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt gcctaaacat
    15241 gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac agattcagat
    15301 acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga taatgttgtt
    15361 tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg tgcggaacgt
    15421 gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga tacgaacgaa
    15481 ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt atctaagcac
    15541 tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga ggttatgaca
    15601 gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc aaatttttgt
    15661 gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc ggaggttgtg
    15721 aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc tcatattttg
    15781 ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt ggaataaggg
    15841 actgcgacaa gac
//
LOCUS       DAFELO010000062        15558 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFELO010000062
VERSION     DAFELO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.064"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(330..632)
                     /locus_tag="LP452_002171"
     CDS             complement(330..632)
                     /locus_tag="LP452_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014873.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LP452_002172"
     tmRNA           complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="LP452_002172"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1389..2162)
                     /locus_tag="LP452_002173"
     CDS             complement(1389..2162)
                     /locus_tag="LP452_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2014874.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2306..2656
                     /locus_tag="LP452_002174"
     CDS             2306..2656
                     /locus_tag="LP452_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL2014875.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2637..3353
                     /locus_tag="LP452_002175"
     CDS             2637..3353
                     /locus_tag="LP452_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2014876.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3353..4801
                     /locus_tag="LP452_002176"
     CDS             3353..4801
                     /locus_tag="LP452_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2014877.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4871..5380)
                     /locus_tag="LP452_002177"
     CDS             complement(4871..5380)
                     /locus_tag="LP452_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014878.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5370..6095)
                     /locus_tag="LP452_002178"
     CDS             complement(5370..6095)
                     /locus_tag="LP452_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014879.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6318..8438)
                     /locus_tag="LP452_002179"
     CDS             complement(6318..8438)
                     /locus_tag="LP452_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2014880.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8705..9847
                     /locus_tag="LP452_002180"
     CDS             8705..9847
                     /locus_tag="LP452_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL2014881.1"
                     /translation="MKKGYPLIQKEDLAHPQKETTQWVELVDSQNQFIAKGYLGKQNK
                     GIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNGEGDGIGGLIVDY
                     YAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNLPESQFIYGKEAP
                     EPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVLNMFSYTGAFSVA
                     AAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEYFKYAKRKQFLYD
                     MIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNAANVSIEQFQTMI
                     EEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH"
     gene            9863..10621
                     /gene="aroD"
                     /locus_tag="LP452_002181"
     CDS             9863..10621
                     /gene="aroD"
                     /locus_tag="LP452_002181"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL2014882.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10644..11129
                     /gene="ybaK"
                     /locus_tag="LP452_002182"
     CDS             10644..11129
                     /gene="ybaK"
                     /locus_tag="LP452_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL2014883.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11200..12453)
                     /locus_tag="LP452_002183"
     CDS             complement(11200..12453)
                     /locus_tag="LP452_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2014884.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12570..13919
                     /gene="gdhA"
                     /locus_tag="LP452_002184"
     CDS             12570..13919
                     /gene="gdhA"
                     /locus_tag="LP452_002184"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL2014885.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14031..15377
                     /locus_tag="LP452_002185"
     CDS             14031..15377
                     /locus_tag="LP452_002185"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL2014886.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5143 a         2754 c         3134 g         4527 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt
      301 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      421 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      481 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      541 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      601 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      661 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      721 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      781 cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta gcgaaatctg
      841 ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta
      901 aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag
      961 gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg
     1021 tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc
     1081 taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa
     1141 ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt
     1201 cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag
     1261 tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa
     1321 taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa
     1381 aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt
     1441 ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt
     1501 ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta
     1561 ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt
     1621 cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa
     1681 tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt
     1741 tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg
     1801 atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca
     1861 tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga
     1921 gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta
     1981 atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg
     2041 gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt
     2101 gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac
     2161 acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac
     2221 aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat
     2281 aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat
     2341 aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca
     2401 ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa
     2461 ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg
     2521 gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt
     2581 tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga
     2641 atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa
     2701 atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag
     2761 aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta
     2821 tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag
     2881 ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca
     2941 caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg
     3001 agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat atcgagacta
     3061 gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag
     3121 gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat
     3181 tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga
     3241 cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca
     3301 tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta
     3361 tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat tgatcgttgg
     3421 agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt
     3481 tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt
     3541 tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca
     3601 aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc
     3661 tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga
     3721 aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt
     3781 gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa
     3841 tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag
     3901 tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg
     3961 gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca
     4021 tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa
     4081 agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc
     4141 ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata
     4201 caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga
     4261 gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat
     4321 cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt
     4381 agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg atgatattga
     4441 tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat
     4501 acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat
     4561 catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga
     4621 tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg
     4681 tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga
     4741 actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta
     4801 aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa
     4861 aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag
     4921 catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt
     4981 ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat
     5041 tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca
     5101 actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca
     5161 aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct ttaagattca
     5221 gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa
     5281 gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa
     5341 cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt
     5401 tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg
     5461 agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg
     5521 aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc
     5581 aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga
     5641 agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa
     5701 agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga
     5761 ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa
     5821 aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg
     5881 ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc
     5941 cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc
     6001 aataaaaaag cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa
     6061 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
     6121 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
     6181 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
     6241 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
     6301 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
     6361 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
     6421 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
     6481 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
     6541 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
     6601 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
     6661 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
     6721 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
     6781 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
     6841 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
     6901 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
     6961 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
     7021 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     7081 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     7141 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     7201 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     7261 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     7321 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     7381 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     7441 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     7501 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     7561 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     7621 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     7681 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     7741 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     7801 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     7861 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     7921 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     7981 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     8041 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     8101 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     8161 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     8221 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     8281 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     8341 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     8401 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     8461 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     8521 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     8581 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     8641 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaaa gagcaagcaa
     8701 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     8761 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     8821 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     8881 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     8941 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     9001 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     9061 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcatag gaggctatga
     9121 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     9181 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     9241 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     9301 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     9361 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     9421 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     9481 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     9541 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     9601 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     9661 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     9721 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     9781 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     9841 ccattagaag gagcgatgca gaatggttcg ggtaaaggga ttgaatatag gaagcggtag
     9901 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     9961 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
    10021 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
    10081 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
    10141 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
    10201 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
    10261 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
    10321 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
    10381 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
    10441 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggttgg
    10501 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
    10561 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
    10621 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
    10681 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
    10741 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa
    10801 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
    10861 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
    10921 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
    10981 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
    11041 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
    11101 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
    11161 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
    11221 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
    11281 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
    11341 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
    11401 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
    11461 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
    11521 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
    11581 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
    11641 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
    11701 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
    11761 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
    11821 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
    11881 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
    11941 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
    12001 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
    12061 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
    12121 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
    12181 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
    12241 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
    12301 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
    12361 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
    12421 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
    12481 atctgaagaa agaaaacaga aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
    12541 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
    12601 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
    12661 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
    12721 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
    12781 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
    12841 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
    12901 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
    12961 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
    13021 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
    13081 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
    13141 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
    13201 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
    13261 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
    13321 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
    13381 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
    13441 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
    13501 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
    13561 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
    13621 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
    13681 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgtccagc
    13741 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
    13801 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
    13861 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
    13921 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
    13981 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
    14041 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
    14101 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
    14161 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
    14221 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
    14281 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
    14341 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
    14401 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
    14461 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
    14521 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
    14581 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
    14641 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
    14701 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
    14761 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
    14821 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
    14881 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
    14941 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
    15001 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
    15061 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
    15121 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
    15181 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
    15241 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
    15301 acaagaaaaa catgttcgca cttctaggca aaccaggctt tgaagatctt gctaaagaat
    15361 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
    15421 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
    15481 attcagaata aggggctgtg acaagacgtt tgtcaggctc tttgtcaaat gatgttggta
    15541 aagagaaatc ttagaata
//
LOCUS       DAFELO010000063        15139 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFELO010000063
VERSION     DAFELO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.065"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..344
                     /locus_tag="LP452_002186"
     CDS             <1..344
                     /locus_tag="LP452_002186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014887.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="LP452_002187"
     CDS             425..1045
                     /locus_tag="LP452_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014888.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="LP452_002188"
     CDS             1746..2030
                     /locus_tag="LP452_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014889.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="LP452_002189"
     CDS             2033..2950
                     /locus_tag="LP452_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2014890.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="LP452_002190"
     CDS             2943..3725
                     /locus_tag="LP452_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014891.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="LP452_002191"
     CDS             3726..4406
                     /locus_tag="LP452_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2014892.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="LP452_002192"
     CDS             4399..5295
                     /locus_tag="LP452_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2014893.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="LP452_002193"
     CDS             5490..7139
                     /locus_tag="LP452_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL2014894.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="LP452_002194"
     CDS             7733..8668
                     /locus_tag="LP452_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2014895.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="LP452_002195"
     CDS             8661..9431
                     /locus_tag="LP452_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2014896.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            9847..10830
                     /locus_tag="LP452_002196"
     CDS             9847..10830
                     /locus_tag="LP452_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL2014897.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            11277..12311
                     /locus_tag="LP452_002197"
     CDS             11277..12311
                     /locus_tag="LP452_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2014898.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(12400..12723)
                     /locus_tag="LP452_002198"
     CDS             complement(12400..12723)
                     /locus_tag="LP452_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2014899.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(12710..13417)
                     /locus_tag="LP452_002199"
     CDS             complement(12710..13417)
                     /locus_tag="LP452_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2014900.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13503..13844
                     /locus_tag="LP452_002200"
     CDS             13503..13844
                     /locus_tag="LP452_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL2014901.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(14015..14863)
                     /locus_tag="LP452_002201"
     CDS             complement(14015..14863)
                     /locus_tag="LP452_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014902.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         4853 a         2579 c         3199 g         4508 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtggagag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
     9601 caaacaaaaa tagctcctcc tatttttcgt ttggttggac ttattaccgc agatgcaagc
     9661 ttttgaacac catttattcc ggaaaatgga ctgtgataag atttttgtca cagtcttttt
     9721 tttgctcaat cagaaacatc ttttaagctg ttcactttgc ttcttttttt gtttcctagt
     9781 ataatgacaa tgtttttgag aaagattgcg acttcactgc ttgatagaat ggagagtaac
     9841 aaataaatga ctactaattt ttggaaagag ctgccaaagc ctttttttgt tttagcacca
     9901 atggaagatg taacagatgt tgtatttcgc catgtcgtaa agcatgcagc agcaccggat
     9961 gtcttcttta ctgagtttac aaactcagac agtttctgtc atccagatgg aaaagacagt
    10021 gtacgaggaa gattaacatt tacagaagat gaacagccga tggttgccca tatttgggga
    10081 gacaagccag aattttttag agaaatgagc attgctatgg cagaaatggg cttccaagga
    10141 atcgatatca acatgggctg cccagtccct aatgtagcag aacgtggaaa aggcagtggc
    10201 ttgatccttc gtccagaagt agcggcacaa ttgattgaag cagctaaagc tggcggtttg
    10261 cctgtcagcg tcaagacgcg tatcggctat acggaaatgt ctgagatgga agaatggatc
    10321 agtcacctac ttcatcaaga tatagccaat ctttctgtcc atttgcgtac gcgtaaagag
    10381 atgagtaaag tagatgcaca ttgggaagtc attcctaaaa tcattgcttt acgtgaccaa
    10441 atcgctccac aaacactaat cacgatcaat ggagatatcc ccgatcgcca gacaggagaa
    10501 gaattggccg agaaatatgg cgttgatggc atcatgattg gccgagggat tttcaagaat
    10561 ccttatgcat ttgaaaaaga cccgagggaa catacagaaa aagaactgat cggactactt
    10621 cgtctgcaac tagatctgca agatcattat tcagaaatca tcccgcgttc catcacagga
    10681 ctacatcgtt tctttaaaat ctatatcaaa ggatttccag gagcaaatga cttgcgagtt
    10741 cagttgatga atacgaaatc tactgatgaa gtacgagaga tcttagagac ctttgaaaaa
    10801 gaacatccaa cgcttttcag tgaacaatga tttcttgctt gaacaccgtg aattagaggt
    10861 cgtgaaaaag ctgtcctgta tcaagtaata agaacagcca aacaaaaata gctcttcata
    10921 ttttgtcgtt ttttgagcta ataccgaggg tcagttgatt gaacaccgtt tatatcagag
    10981 gttgtgagcc tgccgtttaa ctctgagtca caaggagcaa tttcaaaaaa cagcttctca
    11041 tgtttgatga atattttgac ttgtgaccga agagttggta ggagaacacc gtttataaga
    11101 ggttgtgaca aaagtcttgt cgcaacctct tttttaacca aaaaagtgca aatagtttac
    11161 tttaagtaag agaataatta tgatgaataa aaaaggtgga acaaatagca tactatatat
    11221 attgaaatga acactgtatg gatgctccaa cttgatgaag ctaaggagga attcgtatgt
    11281 cagaaaagga attaatgaag gtagtcggtt tttatgaagg gttgccgatc gatgatccaa
    11341 aaagctttat tgatgaaaaa gaacagatac ctgtacccag ttcacgggat cttctggtaa
    11401 aagtcaacgc agtatctgtt aatcctgtcg atacgaaact tcgccaagat aacgggataa
    11461 gaaatgcttt acgtatctta ggttttgatg gagtcgggaa agttgttgct gtaggtgacg
    11521 aagtgcaaaa attttctgtc ggcgatcgcg ttttttacgc aggtacaaca agagcaggaa
    11581 gcaatcaaga ataccaacta gttgacgaac gtattgtcgc tcttgcacca aaaaatcttt
    11641 cagatgaaga agctgcagcc ttgccgttga cctcattgac cgcttatgaa ttgttatttg
    11701 aaaaatttgg tctaactcca gaagaaaatg ccaatcgcgg gaaaaaaatc ttagtgatca
    11761 acggatccgg gggcgtcggt tcgattttga atcaactagc gcattgggct ggcctagaag
    11821 tctacgcgac tgcaagtccg aagaattttg aatggctgaa aaaaacaggt gtagactatc
    11881 caatcgatta tcatcacgac ttaagtaaaa gttttcgtga attaggaatc gatcaagtag
    11941 agtatgtcgc tgtattattc gatattaccc gctactttga tcaaatcaag caccttgtcc
    12001 gaccatttgg acatgtggga acgatcgtag ggatcaaaga gccgttagat ataggtgttt
    12061 ggaaaaacca atctatcagc ttcgactggg agtatatgtt cgctaaaaca gactataact
    12121 atgaaatcga aacgcaagga gaagctttag cgcacattgc tgctttagca aatgaaggga
    12181 ttatccgcag tacattgagc aaggtctatt cagatgggat gaatgcgaag aacttgaaac
    12241 aagcaaccaa agacgtagaa acaggacata tgttaggaaa agtcgttgtt agtggtccgt
    12301 tcaacggata agcaagaata cgccgatttt tttcttattt gactttgata aaaacaagag
    12361 cgcatgacag taatttgcca taagctcttg tttttttatt tataataagc gaatcaacga
    12421 aagggaaaga attcctgcca agacgatcac caataagctt tttgaaagaa tagcagctaa
    12481 aagtgtagga actgaagcca aagcattttc gagattgatc tgcggcagat gtccgatatt
    12541 ttgggtaaat agactgctga accataaagc agacataatg acgatcggga caaaacttaa
    12601 atactcaagc aacggttgcg gtaaatcgaa ttttttcagt aagacaaatg gtaggaccct
    12661 agaaagccaa gttgctaaag tacaacctaa aatagtcagc aatgtaaact caaaagaagg
    12721 catgtttgat ccacaccccc aatccgcacc caatcagtgt tacaactaca atcactaagt
    12781 tgcttgggat aaaaatcagt ccgacatata ccagaatcaa agtgagacca atcaggatca
    12841 actgtaatcg tttagaggtg tttctatcag aaatgacttg aagataaagt aacccgataa
    12901 acatagcgac gatggcgaaa tccaaaccaa actttttcgg atcagtaata aaattcccta
    12961 aaagtgcgcc aacaagagaa gaacccaccc acgtcaaata agaaatccaa ttagctgtat
    13021 tcatccaacg aaagtttaac ttttgtccag tatagttcaa tttattcatt cccagtgcga
    13081 aactttcatc tgtcagcaat gtgccaatca gcagattttg aagcagccga tttttcttga
    13141 aataaggagc aatcgtcata ctcatcagaa tcattcggga attgactaga aaggtcgaga
    13201 aaacgatgga aagcaatggg ctgtgactag caagcatgct gactgtgata aattgggcag
    13261 aaccagcata gaccagcagg gacatcaacg tgacgactag tggatgaaat ccggcagctt
    13321 tcccaacgat accaaatgca agtccaatac cgatataacc gaaaacggta ggtaacgtgt
    13381 ctttgatcgc agtttttata tctaactttt catccaataa caaaactcct ttagcacgac
    13441 ataaattttc ataattgtcg ttaaacttgt gaatattcta aatttttaat gagcatttgt
    13501 caatggtggg attgaaacgt aaagaactag cgggtcgcag atttacacgt attgtttcgg
    13561 tgggaggagg cgcgaaaaat cttgcatggc tacaaatcca agctgatatt ttcgatgcaa
    13621 cggtctattg tttaagtgtg gaggaaggac ctgcattagg cgcaacaatg attgctgcac
    13681 tagggttagg gtggttcgac acacctgaaa gttgttgtca gaattttatc caatatacag
    13741 atgcagtgga acctctctta caaaatgtag aaaagtatca taaaatctat caacaatgcc
    13801 ggaaaatcta tccagcaacg aaggaaattt gtcatgactt ataaaacaaa acgcgtacct
    13861 aatagtcaaa tgactaatat tgggtacgcg ttaagtcttc tttacttcat cctttgcatg
    13921 attcgtgttt ttaaagcaag gtctgctgta tttcttgtgg atttaggatg gttgttatcg
    13981 tttacttgct atttttcatt gaaatcgtct ttctttaccg tttctttagt tttttatcta
    14041 tttttttctt ttggttagaa aattcttttt cccgaaagtc ttctttcagg atttttatag
    14101 agtagcgttt tgaataagag gcgtcagtat gatcgacttg gcagacgaac caagagaaca
    14161 tttgcctttt ttctgcatga ggtgcttctg ctaataattc tttccataaa taataacagt
    14221 cctctatcag aagaagggtt cgttcatcct caggctccca atagaatgtt tcgagagtag
    14281 caatcacttc attgatcaat tcgaatgcgg gaagataggc attttgctga ataagcgatt
    14341 caactgcttc ttggatgaac tttcgcatct ccatgatgaa cttcgctgtt ttcctgtttt
    14401 tgtttttctt gataatatga tcgtatttat ggatgatagt tgttaattta tctgaataat
    14461 ttattgcatg aaatggcatc ttaactttga agcgttcgat aaagtgcgga tgttctataa
    14521 gaatctctgt taaaagctcc cgtacaagag aggaactagc ggaggaaaca agggacgaga
    14581 gttcggaaag tgagtagttg gattttttta ttggacgttc ttcgtaagcg aataagacag
    14641 cagccatgtg tttgcatttt tttccttcta tggcatatag acaatcacaa gtcatgttgg
    14701 tcttttcatc ccaagtgatc actaccttat aatcttccgt gccagaaact gttgcggaca
    14761 agtacttgct tgtatagttc aaatgacgga caaaaccgtg cagatgatag ctgtaaccgc
    14821 gttccaaaac gttttcagga aaatactcaa gccaattatt catctagttc tcctttcatg
    14881 aaacacttat ttatatttag tataaagaaa cgtttaagaa aaaagtagct gattttacat
    14941 atttgataat cacttttgct cgaattgtca aaaattcatt ctccttttct tttcttagag
    15001 aatgtgagaa aaagtaaaag aatagcgtgt tttaaagatg gaactttttt tgttttttat
    15061 ttatctgaca aaaatgaagt gattttgtta gaaataaata attgttccaa ttgataaaag
    15121 aggtatgatg tatcgcaaa
//
LOCUS       DAFELO010000064        14901 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFELO010000064
VERSION     DAFELO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.067"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     misc_feature    112..237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            281..805
                     /gene="infC"
                     /locus_tag="LP452_002202"
     CDS             281..805
                     /gene="infC"
                     /locus_tag="LP452_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL2014903.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            877..1077
                     /gene="rpmI"
                     /locus_tag="LP452_002203"
     CDS             877..1077
                     /gene="rpmI"
                     /locus_tag="LP452_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL2014904.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1130..1489
                     /gene="rplT"
                     /locus_tag="LP452_002204"
     CDS             1130..1489
                     /gene="rplT"
                     /locus_tag="LP452_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL2014905.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1741..3048
                     /locus_tag="LP452_002205"
     CDS             1741..3048
                     /locus_tag="LP452_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL2014906.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3125..5254
                     /locus_tag="LP452_002206"
     CDS             3125..5254
                     /locus_tag="LP452_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL2014907.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5229..5918
                     /locus_tag="LP452_002207"
     CDS             5229..5918
                     /locus_tag="LP452_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014908.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6326..7012)
                     /locus_tag="LP452_002208"
     CDS             complement(6326..7012)
                     /locus_tag="LP452_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2014909.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7148..7342)
                     /locus_tag="LP452_002209"
     CDS             complement(7148..7342)
                     /locus_tag="LP452_002209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014910.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7392..7832)
                     /locus_tag="LP452_002210"
     CDS             complement(7392..7832)
                     /locus_tag="LP452_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL2014911.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7836..8681)
                     /locus_tag="LP452_002211"
     CDS             complement(7836..8681)
                     /locus_tag="LP452_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL2014912.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8830..10248
                     /gene="pepV"
                     /locus_tag="LP452_002212"
     CDS             8830..10248
                     /gene="pepV"
                     /locus_tag="LP452_002212"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL2014913.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10305..11252)
                     /locus_tag="LP452_002213"
     CDS             complement(10305..11252)
                     /locus_tag="LP452_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2014914.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11475..11825)
                     /locus_tag="LP452_002214"
     CDS             complement(11475..11825)
                     /locus_tag="LP452_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL2014915.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12025..13104
                     /gene="pepA"
                     /locus_tag="LP452_002215"
     CDS             12025..13104
                     /gene="pepA"
                     /locus_tag="LP452_002215"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL2014916.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13248..13568
                     /locus_tag="LP452_002216"
     CDS             13248..13568
                     /locus_tag="LP452_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL2014917.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13590..14054
                     /locus_tag="LP452_002217"
     CDS             13590..14054
                     /locus_tag="LP452_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2014918.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14259..14864
                     /locus_tag="LP452_002218"
     CDS             14259..14864
                     /locus_tag="LP452_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL2014919.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
BASE COUNT         4739 a         2749 c         3116 g         4297 t
ORIGIN      
        1 gggaatcact gctagaaagt ctgctaatct aatcattttc agaaaaggaa gacaaactgt
       61 atgaaaaaca ttgacaaaaa gattagtttt tgctatacta tgatggttga gaaataaaag
      121 cagaagcacc cgcttctcgc cttagttgat aatgtcactg ggctagatag ttttcactgg
      181 ttctattagg tgaagtacgc gcgggttctg taagagaact cgttttttta ttgccgttta
      241 ttcggtaagc ttttctttct ctcataatat ttggaggtga atgaccatag caaaggatat
      301 gatggttaac gacggcattc gtgcacgaga gttacgattg atcggttcag atggtgagca
      361 attaggagtt aagacaaaag cagaagcatt gaatattgca gaacaagcaa acttggatct
      421 tgtgttagtt gcccctggtg cgaaaccacc agttgcgcga atcatggact acggtaaata
      481 ccgtttcgaa caacaaaaga aagaccgcga agcgcgtaaa aaacaaaaag tgatcaatgt
      541 aaaagaagtt cgcttaagtc ctacgatcga cttgaatgac ttcaatacaa aacttcgtaa
      601 tgcacgtaag ttcttggaaa agggcgataa agtgaaagct tctatccgtt tcaaaggccg
      661 tgccattacc cataaagaaa ttggtcaaaa agtcttgaat cgcttagctg aagaaactgc
      721 ggatattgca acagtggaac aaaaagcgaa aatggacgga cgcagcatgt tcttgacgct
      781 ggcaccgaaa aacgacagta agtaatttga atgactaact ggctgttgga cagccgatag
      841 tcggataacg aaagattttg aggaggaaat atagtcatgc caaaacaaaa aacacaccgc
      901 ggatcagcaa aacgtttcaa acgtactggt aaaggcggat tgaaacgctt ccgcgcgttt
      961 acaagtcacc gtttccacgg caaaacaaaa aaacaacgcc gtcaattgcg taaagcaagc
     1021 atggtttcta gtggcgactt caaacgtatt cgccaacaac tagcaaaaat gaaataagaa
     1081 gtacaaataa cagaatctga agatttattt ttattaggag gaattataca tggcacgtgt
     1141 taaaggtgga acagtaactc gtaaacgtcg taaaagagtg ctaaaattag caaaaggtta
     1201 ctacggttca aaacacactt tatttaaaac agcaaaagaa caagtaatga attcatacaa
     1261 ctacgcatat cgcgatcgtc gtcaaaagaa acgcgacttc cgtaaattgt ggattgctcg
     1321 tatcaacgca gcagctcgta tgaatggctt gagctactca aaattaatgc acggtttgaa
     1381 agttgctgaa atcgacatca accgtaaaat gttagctgac ttggctgtta acgatgcagc
     1441 agcatttact gcattggctg accaagcaaa agaagctttg gctaaataag ctaaacaatt
     1501 ataaagagga aagaagataa cgaaaagtta tgttctttcc tcttttttta tttacaatcc
     1561 tccatcaaat gaaacaggac aataacgtat aagtctaaaa tagcataatt aaggtttttt
     1621 aattgaaaac tgaacattta tggtcaccct ttaatgaaag cgctaactac ataagttaga
     1681 atgagtttga ctgatatatt agtttgctta tcagaaaaac aaaatggacg gaggaataac
     1741 atgagtgata ttattattgg tattttgtta gttatctcat tctttttcat ggtctggtac
     1801 tgtgtaaaag gctacaactt gatggtcggc tttgccatca tggctactgt atggatggga
     1861 ttagcgctag tcggaaacac gttttcacca aacccggcga tggaagggca aggagtaatt
     1921 gatgtgttga cgcatatcta tacgacgggg ccagctgagt acgccaaatc gatcttggta
     1981 aatgtcttct ttggtgcctt tttcgggcgt gttctagtag atagcggaat cgcagctacc
     2041 ctgattcgaa aggtagttga acttggaggg gacaagccaa gaatcacgat gagcttacta
     2101 tgtattgtaa ccgctgttat attcatgtcg atgacaggaa tcggaccagt gatttctatt
     2161 gcagttattg tgttgccaat cttgatgtca ttaggtattt cagtaccggt tgcactgttc
     2221 tcatttatgg gctcgattat ggctggaatc tttgctaata tcgtcaattt caaacaatat
     2281 caaacgattt atgctggttt caatccagcg gctgaaagct atacttataa tgactatttc
     2341 caaattggta tgattgggat gattgtaagc ttagttgtgg tactaacggt cgctaatata
     2401 tcaatgaaca aaaagaaacg ctatgcaatg gcagcaaacg taccagctga aggtggcgac
     2461 gcaccaatga tctcatggtt agccgtatta ttaccagttt taggggtcgt tcttttagat
     2521 cttccaatca tcttaggttt tattctagca ggtatctggg cattgctgtt tactggaaaa
     2581 ttgcgcggag gttataaaga aatctgtcgt cagtttgcga aattatttac agatggcgca
     2641 gtagatgttg caccaatggt tggtttcttg atgaccttag cgatgttcaa taattcagct
     2701 gcgtatgctt ctccttattt ttcagctatt tttggagatt ggataccgca atcaccatta
     2761 gtcttggcaa tcgtatttgc tatattgaca ccactaggat ttttccgcgg tccaatgaat
     2821 ctagtcggtt caggctcagc tattttagct gtcgtattag ctgttaatcc aacgatgtcg
     2881 ccagcctttt tgttcccgtt atttgcaatc acaacgattg ctcctcagca tttagatatt
     2941 acacaatctt gggttgcttg gggattaggt tatacaaaag taacttctcg agaatatatg
     3001 aagaaatcca tcccaactgg ttggatcatc ggagcgatct ttgcctgatc acgttcttat
     3061 tgtacgggaa cgcctagtaa acaaaacgtc gataaaaacg attagatgaa tggaggaaaa
     3121 gaaaatgaaa cgttttgtgc gaatgggaat tgatgttggc ggtacacata cgaaagcagt
     3181 cgcaatcgat aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa caacccacga
     3241 tgatgtccgt ggagtagccg caggtgtcgt tcagtctttt caaaattgtt tgcgggaaaa
     3301 taatatcagt ccagaagatg ttgtgtttgt tgcacattct accacacaag cgactaatgc
     3361 actgattgaa ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag gtggattaga
     3421 aggtttttta gcaaaacaac aaactagatt gaacgatatc gatttaggga ataagaaaaa
     3481 aatagagatt gtcaatgcat tcttacctgt caaacactta aatgtagacc gtgtttcaga
     3541 aacgatttct tctttagaaa gagaaagagc ggaggtcttg gtttcttcta tggcatttgg
     3601 agtagataat ggggaaccag aacgagttgt ttatgaagca gcatcggtaa aatcgatccc
     3661 tacaacgatg gcttctgaca tcacaaaatt atatggattg acaagaagaa ctcggacagc
     3721 agcaatcaat gcatctattt taccaaaaat gctagatacg gcaacctcta cagaagattc
     3781 cgtacgagaa gcaggggtca atgtttcttt gatgattatg cgcggagacg gcggagtaat
     3841 ggaaatcaat gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc cagcagcatc
     3901 agtcatgggc tctttgatgt acttgagagc gtctaatggt gtttattttg aagtaggcgg
     3961 aacaacaacc aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt attcaattgt
     4021 aggtggccac ccaacatata tctcttcatt agatgttcgg gttctaggtg tggcaggcgg
     4081 ttcgatggta cgggctaatc agtcaggaat catcgatgtc ggtccacgtt ctgcacatat
     4141 tgccggtcta gattatgcag tatttacgga gacagaaaaa atcaaaggtc ctaaggttga
     4201 atttttctct cctaaagaag gagaccctgc tgattacgtc aaggtagtga tggaagacgg
     4261 agaagaagtg actattacaa atacatgtgc agccaatgtc cttggattag tgcaagaaga
     4321 acatttttct tatggaaatg tcccatctgc acgaaaagcc atccaagcat tagctgatta
     4381 ttgtcatacg acggttgaag acatcgcaga acagatcatg gaaaaatcct atgctaagat
     4441 tgaaccggtg attttagaat tggcagacaa gtatcacttg gagaaagatc agatttctct
     4501 tgtaggtgta gggggcggcg ctgcctcatt gattacttac tttagtaata aaatgggtgt
     4561 gaaatattcg attcctgaaa acgccgaagt gatttcttct attggtgtag cacttgcaat
     4621 ggtccgagat gttgtagaac gtatcatccc ttcgccaagt aaagaagata tacgatcgtt
     4681 gaaaaatgaa gcgatgaata aagcgattga atcaggcgct acaccagaat cgatcgaagt
     4741 ccacgtagaa atcgatccac agacttcaaa agtaacggct atcgctactg gttctacgga
     4801 agtgaaagca accgatctaa ccaaggagat cacaacagaa gaagcactag agcttgctgc
     4861 agaggatatg cgattaaata aaaatgaagt ttgcttattg gaaaatacac ctttctttta
     4921 tgtatgcggc gaacagaatc gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa
     4981 aggatttatc aaggtccaac gtggacatgc ttcatgtatg aaaacaactg cagccaatta
     5041 tatgacagct gtcgaacaac tttgggaaga tatggcagtt tatcaaactg aattgattgc
     5101 ccgaccagaa ttttatctct gcttaggagc aagagtctct gattttacag cgacggatct
     5161 ggaacaacta cagcttttga tggatctgga agtatctact atggaaccgg aggaagaagt
     5221 gatcgtcgtt gccggaaata tcaaacaaac ataagttaga agagttacta aacgaattac
     5281 aacagatccc cgaagaaatt tggggattct accagtttcg gcgagattta ttttggaaaa
     5341 agattccttt gtccaagcag aaaattctta tacaacaatc aatcgattgt ggtatcgaaa
     5401 cggcatgtag catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa
     5461 tggctgttcc aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa
     5521 cttatgcaga agatgaaggg attcgattga tgacggagcc gttagaaaaa ttaaaatgtt
     5581 caggactagc ttcgatctct aaagaaacag cgcaagcttt gattattgga catgaactat
     5641 ttcaccacat cgaagcttct gttaagggaa tttatacgca aagcgaaaaa attgtgctgt
     5701 ggcggttgcc tttttataca catcaatcaa acattcgtgc attgagcgag atagcagcga
     5761 tgagtttttc aaaagagatg aatcaaagcg atttttcgcc ttatgtatta gaggcagtct
     5821 tactttggcc atataatgaa attcgtagcc aagggatctt agaagaaata aaaaaaatag
     5881 aaaaaagatg tacggagtat gatttagatt acaaataaag agactgtgac aaaagtcgtg
     5941 tcacagtcct ttttttccga ataaacgatg ttctcctgtc aactcctcgg tcacaagtca
     6001 aaatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagatga
     6061 acggcaggct cacgacctct tggaaaacgg tgttcaaaag cttgcatctg cggtaataag
     6121 tccagccaag ttaaaaacat aagaagctat ttttacttgg ctgttcttat tacttgatgc
     6181 agggcagctt tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta
     6241 gctcaaaaaa aacggcaaaa tatgaggaac tattttgccg tttctttctc taatactcag
     6301 gtcaaaacgc tgattccaaa acctcttatt tgatgggtct gtcctgaata tcttcaaaat
     6361 aatcggggta attatgaatc acgtattctt gctcctcgat aatcatatgt aaatgcatcg
     6421 tgcaaagaaa tccgacttca tcaaagttat gatcaatcat tgattgaagc attgtctgat
     6481 gttcatctaa tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac catctgaaac
     6541 ggttcaagtg cgtactataa ctttctagcc aatcccaaat ttctcgtttt ccagcaattt
     6601 cataacaagt tcggtgaaaa tcagtatcca aatcaaaaaa agctttttta ttttgagcga
     6661 ttactgcctg atcggtttgt tctagtattt tttctagttt agcttgatct tgcttggtca
     6721 ttttcgcact gcattcttgc aggatcggtt tttccaactt ttcccgcgta taacgggcta
     6781 atgaagcaga tcggatatcg atttttgaaa cataagtgcc ggactgtgga atggttttta
     6841 caagctcttg attgcgcagt tggatcagtg cttctcgaat cggtgtacgc ccgatagcta
     6901 gttgttcttc taacacttta tcggagattt tacttccagg tgctaactcg gaataaatga
     6961 tttgtttacg tatagattca taagcttgct gttgcaaatt attgataacc atttaacctt
     7021 cctttctatg cagatataat ctattataac ataaatcgct tacaaatcgt gtgggcataa
     7081 aaacacagtc tgcaaaaaaa gccctgatag gcatgcctat tagagcctcg aaaagaaaaa
     7141 tattgaatca atctggtcgt acctcttgta tcggtttgac acgaccgccg attcttttag
     7201 caaaagcttc ggattctttt ttgtctttga aaatgagcag attgtctaag tttttttccc
     7261 gagtgcgaca gccagtttta tcgatgtaac cgtgcattac tttaacgaca tacatgggga
     7321 tcacctgctt ttttcttttc attatacgaa ctactcgaca caatacaaga aaaatgctta
     7381 gacggtcttt attaagaatt tacatcatcc aaagaagcgt acgcttggaa taactgatct
     7441 aactccggat tgactttgct cagcttcact aatcgttgcg aatcagaaag taaaaaacag
     7501 tgtttactac tgccagtagt ttttaatttt ttgtcttcag ttcccacaat ttcgtagcta
     7561 aaatccaacc gtgtccccgt atacttttgg atagttggat ggatgctcac tttttctccg
     7621 taatggatca tttctttata gctgcaattt acttccagaa cggggatgat gatcccagat
     7681 tcttcgattt ttttataggg aaaccctaga tgtaccaata aggctacccg agcctcttca
     7741 aaccagcgga tataattgct gtgatgaata atccccattt gatctgtttc ataataaaaa
     7801 ggttctcgca cataacctgt aaatttctcc atgactcatg cctcgttttc cattgatttt
     7861 ttacaaaaat aattcatcca tttcggaagt gcttcgctga tttgtgtcgg caaaacaata
     7921 tattgttctt ttgccagctg gtctccaatg aaactatgaa gataaatagc tgctgcaatc
     7981 gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga tcatcccagc taaagtgtca
     8041 cccatgccgc ccgttgccat agcagcagaa cctaaaggat tggtgtagaa gtgctggtga
     8101 ccataaattt ttgtctcatg acttttcaaa acgagcacag caccgatttc ctgttgcttc
     8161 tttttgtttt ctttatcccg ttgttcttgt atcggtagag aactcaaccg ttgccactcc
     8221 atttgatgag gtgtaagtac agtttgttcg ggatagggta gcaaaagatg attagcagca
     8281 agtaaactta aagcagagcc atcaatcacc agccattgtt cttttgattg gttttctagt
     8341 acttgtttga aacgttccaa actcttagaa gaagtaccca atccgggacc aatcaagagt
     8401 acatctgctt gctttattac ggaaagaaac tggtcagtat ccgtccaaac tacaatcatt
     8461 gcttctggca gatgactgtg aagagcactc cgattttttt catcagttac tacagtcgtg
     8521 agacccgctc cgctgtgtac ggcagctaaa gcactcataa tgattgctcc gccatattgt
     8581 tcattgccgc caatcaaaac gatacgtcca aatgttcctt tatgtgaaat ttttggacga
     8641 ggtgtgatga ttcttttcac tagttgttcg tttatcagca tctaaaaacc tccttttact
     8701 cattatagcg tatatttttt ttagagaaaa acagataaga aaaggaattg tcagattttt
     8761 tgaactattt ttttctgtaa tttgtggtat catgaacacg aagacttaaa taagtggagg
     8821 attaacttta tgacaattga ttggcaaaaa gaagtagaag cacgtaaaga agacttgctt
     8881 gaagacttga aaaatctttt acgtgtcaac agcgagcgcg atgactcaaa agtaacacca
     8941 gatgcaccat ttggacctgg acctcgcgac gcattgaaac atatgttagc ttatggcgaa
     9001 cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag ggcatattga tcttggcgaa
     9061 ggagacgaaa cgctagggat atttggacac atggatgtcg ttcctgcagg agatggctgg
     9121 gacacagatc cttatgaacc agtaatcaag gatggaaaga tctttgcacg cggatcaagc
     9181 gatgacaaag gtccaagtat ggcagcttac tatgcgatga agatcattaa agaacttgac
     9241 ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg atgaagaaag cggctggggc
     9301 gatatggctt attatttcga acatgaagaa gaaccagatt ttggtttctc accagatgct
     9361 gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt tagctttacg cttcaaaggc
     9421 gacaatgcag gagattatgt cttgaaatct tttgtttccg gacttcgcga aaatatggtg
     9481 ccaggaacag caactgcagc acttcaagta ccaagtgcag atgcagctat cgcgatggaa
     9541 gaagcatttt accaattcat cgaagcaaat ccagtaagcg gaacgattga agcggacaat
     9601 acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg gtgcaagccc acaatctggt
     9661 atcaatgcag gttctttctt ggcattgttc ttagataact atgaattcct aggatctgct
     9721 aaacaattta tccatgtagc agctgcttat gtccatgaag atttttatgg agaaaaacta
     9781 ggagtcgctt atgaagatga aaaaatgggc aaattgacaa tgaatgctgg actatttgct
     9841 tttgaagaaa atggtacaga ggaagcgaac ttcatcaata tgaatttccg ttttccaaaa
     9901 ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa cagtggggca agaaggcgca
     9961 acagttactc gaggtgctcg cgtaatggaa ccacattacg taccaatgga tgatccattg
    10021 gtggctacat tattgcaagt ctatgaagac cacactggag aaaaaggcta cgaacaaatc
    10081 atcggtggcg gaacttacgg acgtttgttg aaacgtggcg ttgcttatgg agcgatgttc
    10141 ccaggatata ctgatacaat gcatcaagca aacgaattta tgagtcttga tgacttgttc
    10201 agagcaacag caatctatgc agatgccatc tatcgtttgg cgaaataatt aattaaaaag
    10261 aggatcaaga attgcaaatt atttttgcag tcttgatcct ttttttactt tctactgtgg
    10321 tattcatgat aaacatgata tagattcaat tcatattctt tcttagcgtt cgtgacgact
    10381 gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta aagtcatcaa tccagtactt
    10441 aaaatcgcag aagggatgcg tgtaataaag cttgtcagca tatattcgcg aatcacagga
    10501 atccccgtga ccaatccaaa taaaaagaag aagatggtcc aaatcccaaa gaaaagcaaa
    10561 ggcttataat ctttaaacat tttaataatc atcatgatca ctttgaatcc gtcagagaat
    10621 gtattcaatt ttgattcgct gccttcagga cgatcacgat aatcgattgg gatttcaacg
    10681 aacttgaatt ttttatctaa agcatggatc gtcaattccg tttcgatttg aaaaccagaa
    10741 ctcataatag gaaaactttt gacaaagata cgattgaatc ctcgatagcc ggtcatgaca
    10801 tcaagaattt ttgttttata cattctagta attgtatttc taacaaggtt atttccaaaa
    10861 tcgtggaaag gtctcttatt ctcatcaaag taggttccat ttgataggcg atcaccaatg
    10921 accatatctg cttcaccaga acgtaatttc tcgagcaaac catgtacagc ttctgcagga
    10981 taagtatcat cgccatctac cattaaataa tagtcggcat cgatatcaaa gaacatttgg
    11041 cggatcacat ttccttttcc ttgacgaggt tcttttttta cgattgctcc accttgaacg
    11101 gccaattcgt atgttcgatc ggtcgaatta ttatcataga cataaatatc tgcttctggt
    11161 aattctcgtt taaagtctgc aatgactgtt gaaattgttg cttcctcatt gtaacaagga
    11221 atcagaacag caattttttc ttgttgttcc atttttcact ctccttaact atagaaaaca
    11281 aaagctaaat taaaaatacc tcataatcaa aggaaatgct agtttttccc gctaaatatc
    11341 atttatttaa aaagaaaata ctggaatata tgtttggaaa gctctatcta aaaaaccacc
    11401 cactacaaaa gaaggacatg atacctctct tttgaatagt gggtgcgtat tcgttctctc
    11461 acttttttga cgtattaatc tgttgcacct ttttttcttg aaatttctaa tacggttcct
    11521 gtatgagcat ctgctaaaaa ttcataataa acagtttcgc catcttctat tcgagaaaga
    11581 cctccccgat aagcactcga ccgaacggcg aatttcctaa taggttcctt ttcaaaagag
    11641 atccaagaac cttcgattgc accttctttt agaaaagatg acttgatatt ttcaagtact
    11701 tggtctgctg acaatacttt tttgcgatga taccaaatcg ttgaagctac cccgctcaat
    11761 aatcctagac cgattccaac tgctaacccg cctttaaaat agcttagttc gctttcttca
    11821 tacatgtgga catacctcct tcttctcact tgttactcct tgtgcttatt ttaccacact
    11881 ctcatatttt ctttgaaata aataaccgct accagaaaaa ataagctata ataaatacga
    11941 tcggtctgca gaaaaaatca tattttatcc gcagatcaat ataagtctga gaggagttta
    12001 gtctgaaatt tagacgaata gcatatggaa gaaaaaacat ttcaacgaat caaagagtta
    12061 acagaattac aaggaacaag cggttttgaa caagatgttc gcgcttatat ggaaagtcat
    12121 atggaaccgc tggtagatga gttacagtta gatggtttgg gcggcatttt tggtctgcgc
    12181 catcatgaag aagcggatgc accccgtgta atggtcgcag cacatatgga tgaagtcgga
    12241 ttcatgctga cacaaatcca agaaaacggg ctttttaaag tcgtacctct aggcggctgg
    12301 aatccttacg ttgtttctgc tcagcgtttt acacttaaaa cctctactgg taagaattat
    12361 ccatgtattt cttcttccgt tcccccacat ttattgcgag gaacaagcgg acaaaaatca
    12421 gtagaagtaa ccgatatttt gttcgatgct gggtttgaat caagagaaga ggcaatgagt
    12481 tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg aaacgatcaa aacagccaac
    12541 ggaaagaata ttatcagtaa atcatgggac aaccgctatg gctgtacaat ggtattagaa
    12601 gctttagaag cgttgcaaaa tgaaacatta ggtcatacat tgattgcagg cgcgaatgtc
    12661 caggaagaag taggcttgcg tggttctaaa gcttctgtca ataaattcaa gccagatctg
    12721 ttctttgcag tagactgctc tgcagcagat gatacagtga cgaaaaatgg cacattcgga
    12781 catttaggag aaggtacatt gatgcgtatc caagatccag gtttgatcat gcttcctcgt
    12841 ttgcgcgagt acttgctgga tatcgctgaa acaaacaata tcccttatca atactttgtt
    12901 tcaaaaggcg gtacagatgc aggtgctgct catacacaaa acgaaggaat cccaagtaca
    12961 gtgattggcg tagtgggacg ttatatccat acgcatcaaa cgatgttcag tattcgcgat
    13021 tttgaagctg cacgagaaat gttgattcaa acactgaaag gtttggataa atcaacagtg
    13081 aatacgatcg tatacggaaa ataatgcgat agtgaaacta taaaatctgg caaaagattg
    13141 ctttttgcca gatttttttt ataaaaagag gatcaatgtt tgttagttcg aaagaaaggt
    13201 gtataattta attgaaaaat acataaacag aaagtagagg acaaagaatg attatcccaa
    13261 atagtttaga agaattagca ggctacgtag aaactggaaa gagtgtattt ttctttactg
    13321 ccggctggtg cggagactgc cgttttatca aaccgcaaat gcctgaaatt gaaaatgact
    13381 tttctgattg gcaatttatt gaagtagacc gtgacaagta catagatgta gccgctgaat
    13441 ggaacatttt tggtatccca agttttgtcg tgatccaaga cggaaaagaa ttaggtcgtc
    13501 tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc gttcctagaa agtgtaacaa
    13561 aaggctgaca tctgttagga ggggtcaaga tgaatcaaga atataagaat attttagtag
    13621 gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa agcggtagaa gtggctcgcc
    13681 gtaataatgg gaaagtatat gtagcaaacg taatcgatca gcaattttat aactttatgg
    13741 gctatgcacc tattgatcaa aatctagtcg atcagcaaac tgaaacagcc aaggaaatga
    13801 tcgaagaatg taaagaatat gggaaatctg ttgattacac aaatatagaa ggaattattg
    13861 catatggttc tgttcgtgaa tccatggcag tcaaattgcc aaaaaaatat gagatcgatc
    13921 taatcatggt tgggcagtca ggtctaaatg cagtcgaacg ctttatgaca ggaagtgtag
    13981 ccagctacgt gatacgtcat tcaccttgtg atgtcttgat cgtttctgac gaagaagaac
    14041 agccaagtaa ttaagcaagt ggaacttgta cgaccttttt aatcaattaa ctaaaacagt
    14101 aaagggactg tgacaaaagt cttgtcacag tcccttgtta cgaataaatg gtgttccaga
    14161 agaggcttct tcggaaataa agcatgaaaa aaatttcatg cagtgaatgc aattttttgt
    14221 taagataaat ctcagcaaag gataatggag gaaagaacat gatttttgct tataacaaag
    14281 cacacgtcgg agacaccttg atggttatta tagccgacga taaaggaaca gaaaacactg
    14341 ttgaacgcaa atggaatgtt gctcggatca aagatgaaca aggtcaagtt gttgcttgga
    14401 actttttcca tatctccgat catctaacaa tcgaagggaa cggccaagta agcttaacag
    14461 atgaggaatt agcgaaattg aatcgattga tcaaagaagc aggattcgaa gaacagcttg
    14521 aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa aacatgcgtc ccacatcctg
    14581 attctgacca tttatcgatt actgagacag aagtagacaa tggtacagta ttgcaaatcg
    14641 tttgcggtgc acccaatatc gaagctggac aaaaagttgt cgttgcaaaa ccaggagcta
    14701 tgatgccaga tggaatgatg atctggccag gaactttgcg aggggtagat agctatggaa
    14761 tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc agcaaaaaaa ggaatcttag
    14821 tattgcctga ggatgccgta gtcggagaag catttccaaa ataaagaggt tgtgacaagt
    14881 ctttgtcaca acctcttttt t
//
LOCUS       DAFELO010000065        14548 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFELO010000065
VERSION     DAFELO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.068"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            4..816
                     /locus_tag="LP452_002219"
     CDS             4..816
                     /locus_tag="LP452_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL2014920.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LP452_002220"
     CDS             813..1613
                     /locus_tag="LP452_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL2014921.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LP452_002221"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LP452_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL2014922.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LP452_002222"
     CDS             3110..4819
                     /locus_tag="LP452_002222"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL2014923.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="LP452_002223"
     CDS             5031..9389
                     /locus_tag="LP452_002223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL2014924.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="LP452_002224"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="LP452_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL2014925.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="LP452_002225"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="LP452_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL2014926.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="LP452_002226"
     CDS             11227..11520
                     /locus_tag="LP452_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL2014927.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="LP452_002227"
     CDS             11517..11828
                     /locus_tag="LP452_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL2014928.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="LP452_002228"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="LP452_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL2014929.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLTSLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="LP452_002229"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="LP452_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL2014930.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4960 a         2583 c         3237 g         3768 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttacaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaa
//
LOCUS       DAFELO010000066        14441 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFELO010000066
VERSION     DAFELO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.069"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            282..2753
                     /locus_tag="LP452_002230"
     CDS             282..2753
                     /locus_tag="LP452_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2014931.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2856..4592)
                     /locus_tag="LP452_002231"
     CDS             complement(2856..4592)
                     /locus_tag="LP452_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL2014932.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4589..5293)
                     /locus_tag="LP452_002232"
     CDS             complement(4589..5293)
                     /locus_tag="LP452_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2014933.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5424..5927
                     /locus_tag="LP452_002233"
     CDS             5424..5927
                     /locus_tag="LP452_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL2014934.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5887..6225
                     /locus_tag="LP452_002234"
     CDS             5887..6225
                     /locus_tag="LP452_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL2014935.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6246..7664
                     /gene="ffh"
                     /locus_tag="LP452_002235"
     CDS             6246..7664
                     /gene="ffh"
                     /locus_tag="LP452_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL2014936.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7776..8354
                     /locus_tag="LP452_002236"
     CDS             7776..8354
                     /locus_tag="LP452_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL2014937.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8358..8879
                     /locus_tag="LP452_002237"
     CDS             8358..8879
                     /locus_tag="LP452_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2014938.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8958..9512)
                     /locus_tag="LP452_002238"
     CDS             complement(8958..9512)
                     /locus_tag="LP452_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2014939.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9765..10040
                     /gene="rpsP"
                     /locus_tag="LP452_002239"
     CDS             9765..10040
                     /gene="rpsP"
                     /locus_tag="LP452_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL2014940.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10052..10303
                     /locus_tag="LP452_002240"
     CDS             10052..10303
                     /locus_tag="LP452_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL2014941.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10428..11219
                     /locus_tag="LP452_002241"
     CDS             10428..11219
                     /locus_tag="LP452_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014942.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11389..11910
                     /gene="rimM"
                     /locus_tag="LP452_002242"
     CDS             11389..11910
                     /gene="rimM"
                     /locus_tag="LP452_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL2014943.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11900..12661
                     /gene="trmD"
                     /locus_tag="LP452_002243"
     CDS             11900..12661
                     /gene="trmD"
                     /locus_tag="LP452_002243"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL2014944.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12797..13144
                     /gene="rplS"
                     /locus_tag="LP452_002244"
     CDS             12797..13144
                     /gene="rplS"
                     /locus_tag="LP452_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL2014945.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13402..13695
                     /locus_tag="LP452_002245"
     CDS             13402..13695
                     /locus_tag="LP452_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL2014946.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4843 a         2593 c         2932 g         4073 t
ORIGIN      
        1 attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa
       61 agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt
      121 ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg
      181 tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag
      241 tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa
      301 atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc
      361 catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag
      421 caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat
      481 ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct
      541 ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg
      601 aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg
      661 tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg
      721 ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc
      781 aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc
      841 cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg
      901 gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac
      961 agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc
     1021 ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa
     1081 gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga
     1141 cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca
     1201 atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa
     1261 cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa
     1321 tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag
     1381 acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc
     1441 accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg
     1501 tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag
     1561 agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga
     1621 ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta
     1681 atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca
     1741 tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga
     1801 tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag
     1861 ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc
     1921 aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag
     1981 ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg
     2041 cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag
     2101 gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt
     2161 tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat
     2221 cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag
     2281 aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc
     2341 caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga
     2401 atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc
     2461 tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct
     2521 gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta
     2581 tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta
     2641 ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc
     2701 cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag
     2761 tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc
     2821 atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg
     2881 aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg
     2941 tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg
     3001 tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca
     3061 aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg
     3121 tcgctttttg aatattggac agcattttca atcagatttt tgataattgg ataaaggata
     3181 tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt
     3241 tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc
     3301 tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta
     3361 tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca
     3421 tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt
     3481 aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc
     3541 gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc
     3601 aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga
     3661 tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg
     3721 taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc
     3781 agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt
     3841 aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg
     3901 ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact
     3961 tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc
     4021 tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga
     4081 aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac gcttgtcggt
     4141 tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat
     4201 aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc
     4261 aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg
     4321 tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg
     4381 aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg
     4441 atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga
     4501 aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag
     4561 aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa
     4621 atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca
     4681 aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc
     4741 ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta
     4801 attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg
     4861 tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt
     4921 gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac
     4981 taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt
     5041 ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct
     5101 gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg
     5161 attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc
     5221 cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta
     5281 gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag
     5341 aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc
     5401 tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct
     5461 gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag
     5521 aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg
     5581 aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa
     5641 gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc
     5701 aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt
     5761 cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt
     5821 attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg
     5881 agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc
     5941 ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta
     6001 ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg
     6061 acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt
     6121 cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa
     6181 aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag
     6241 aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa
     6301 aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct
     6361 tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac
     6421 gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg
     6481 ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa
     6541 aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg
     6601 gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg
     6661 acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc
     6721 ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt
     6781 tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg
     6841 atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt
     6901 tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc
     6961 agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg
     7021 cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa
     7081 aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg
     7141 gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag
     7201 aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg
     7261 atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc
     7321 aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga
     7381 tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc
     7441 gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac
     7501 aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag
     7561 gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc
     7621 gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct
     7681 tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta
     7741 ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa
     7801 cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa
     7861 agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt
     7921 taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa
     7981 attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa
     8041 gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg
     8101 gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc
     8161 attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa
     8221 agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg
     8281 ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca
     8341 gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct
     8401 atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa
     8461 ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc
     8521 aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt
     8581 tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg
     8641 cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc
     8701 gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc
     8761 ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg
     8821 aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga
     8881 agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt
     8941 tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa
     9001 atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc aattagattc
     9061 gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt
     9121 gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg
     9181 actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa
     9241 gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg
     9301 tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt
     9361 tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct
     9421 tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa
     9481 ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat
     9541 aataatctta caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac
     9601 atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa
     9661 aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt
     9721 tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc
     9781 gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac
     9841 gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc
     9901 ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac
     9961 cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag
    10021 ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac
    10081 aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa
    10141 agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa
    10201 acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc
    10261 gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc
    10321 gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa
    10381 aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa
    10441 tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag
    10501 acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta
    10561 ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg
    10621 ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct
    10681 cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt
    10741 acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg
    10801 caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc
    10861 catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc
    10921 agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa
    10981 tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt
    11041 atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg
    11101 gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga
    11161 tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa
    11221 atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata
    11281 gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc
    11341 ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac
    11401 ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca
    11461 acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac
    11521 ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta
    11581 ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta
    11641 aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc
    11701 atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca
    11761 ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca
    11821 tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt
    11881 ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga
    11941 aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa
    12001 tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg attatccata
    12061 cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat
    12121 tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg
    12181 attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg
    12241 tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg
    12301 agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg
    12361 gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg
    12421 tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga
    12481 agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag
    12541 aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac
    12601 gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg
    12661 actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg
    12721 caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa
    12781 taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc
    12841 tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg
    12901 tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat
    12961 cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc
    13021 attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc
    13081 aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg
    13141 ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct tttttcttag
    13201 catgtaattt gaacaccctg atccagtttt ttctttaatt attggtaggg gagaataatg
    13261 gattagaata aatctaccta taagtagtag taaaaaaaag agcactatga taatatcttt
    13321 cttaagagta accgctttaa taaaaaatcc agaatattct aacataaatg gagtattctt
    13381 agaatcaaaa ggagaaagaa gatggtttat acttatacgg agcaagaaaa gatgaaagtt
    13441 ttagacacat tcatgaatga tggaaaagta aaacaagtac caagttcaac aaaaaagaaa
    13501 tacatcctat tgaaagaagt cttgaaacgc tttgaccatg gagtgactta tacagaaaca
    13561 gaggttaatt cgatactgct gaacgtattt tctagtggcg attatgtcga acaaagacgt
    13621 tatctaatca catttggctt tttcaaaaga agttcagacg gtcgggcata tcagatgatg
    13681 ggaattgaaa attagaagtt ttaatttata ctcattgaaa aaagttcaag tatgcgatat
    13741 tggttctaca ccgcatactt gaactttttt tgtcatctgt atctataata caaagccaag
    13801 aacgttattc aaaatatgga tagctatgat aaaagctaat gagcgattcg cagttaaata
    13861 agcaatcgtc agtaaaatcg ttccaggagc gtacatgaaa aaatcaattg gatgcgttac
    13921 atgaataaag ataattctct tttatttgta cgaataaata ccaaaacgta cctcctaaaa
    13981 ttagattttg gacagatgcc ctttaaaaag ataacaagat ttcgttcttg aaacaatata
    14041 tatgctttta ttccaatgag gttgtgaaaa cagtattttg tcctgagcgt tagaaaattg
    14101 aaaatgggga atagcaactc gtactttacc attttttaag tgaatacaag atcagctaat
    14161 tgaacaccgt ttattcgtga tgagggacac aacactgcag tcgacacatt tctcttttat
    14221 gttcaaagat aacaaatatt taaaacaaag aaaaatagta gataatcccg tcctaactct
    14281 tttattcttc cctcatagct tccaaattta agcaatactt caattaatca agttttatct
    14341 ttttttttca ataatcgtaa aaaaagagta ttttttattt aaaaataaaa tctttgtatt
    14401 aaaggacaag actaaaataa ccttagaata aaaagagaaa a
//
LOCUS       DAFELO010000067        14238 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFELO010000067
VERSION     DAFELO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.070"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP452_002246"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP452_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL2014947.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP452_002247"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP452_002247"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL2014948.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LP452_002248"
     CDS             complement(2169..2906)
                     /locus_tag="LP452_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL2014949.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LP452_002249"
     CDS             complement(2997..3524)
                     /locus_tag="LP452_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014950.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LP452_002250"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LP452_002250"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL2014951.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LP452_002251"
     CDS             complement(4401..4886)
                     /locus_tag="LP452_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL2014952.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LP452_002252"
     CDS             complement(5039..5236)
                     /locus_tag="LP452_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014953.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP452_002253"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP452_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL2014954.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP452_002254"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP452_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL2014955.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LP452_002255"
     CDS             9211..9423
                     /locus_tag="LP452_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL2014956.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LP452_002256"
     CDS             9425..11104
                     /locus_tag="LP452_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL2014957.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LP452_002257"
     CDS             complement(11358..11558)
                     /locus_tag="LP452_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2014958.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="LP452_002258"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="LP452_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="LP452_002259"
     tRNA            complement(12249..12322)
                     /locus_tag="LP452_002259"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="LP452_002260"
     CDS             complement(12634..13032)
                     /locus_tag="LP452_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014959.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="LP452_002261"
     CDS             complement(13025..13477)
                     /locus_tag="LP452_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014960.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13970)
                     /locus_tag="LP452_002262"
     CDS             complement(13464..13970)
                     /locus_tag="LP452_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014961.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            14075..>14238
                     /locus_tag="LP452_002263"
     CDS             14075..>14238
                     /locus_tag="LP452_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2014962.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
BASE COUNT         4211 a         2989 c         2399 g         4639 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatact
    13861 tgtaaaaaat tatctgtaag atgttcatct tcaaagatag tttcttcgag atatttaatc
    13921 gccaattcta aattgctatc attttgttga accaaccact taatgtccat ctcactaaac
    13981 gagagtgtaa aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt
    14041 accacaacac attcacagaa aagaggactt ccatatgaac gattttacta cagaaattct
    14101 aaagactcta gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggaaaaagc
    14161 tgtcaatacg cttctcaaaa cggagttaac ggctttcctc gattacgaaa agtacgatcg
    14221 cattggtttt aacacggg
//
LOCUS       DAFELO010000068        14110 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFELO010000068
VERSION     DAFELO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.071"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..153
                     /locus_tag="LP452_002264"
     CDS             <1..153
                     /locus_tag="LP452_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2014963.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="LP452_002265"
     CDS             159..575
                     /locus_tag="LP452_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014964.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="LP452_002266"
     CDS             681..944
                     /locus_tag="LP452_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2014965.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="LP452_002267"
     CDS             944..1216
                     /locus_tag="LP452_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2014966.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="LP452_002268"
     CDS             1293..1622
                     /locus_tag="LP452_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2014967.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1899
                     /locus_tag="LP452_002269"
     CDS             1612..1899
                     /locus_tag="LP452_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2014968.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            2185..3042
                     /locus_tag="LP452_002270"
     CDS             2185..3042
                     /locus_tag="LP452_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2014969.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            3043..3591
                     /locus_tag="LP452_002271"
     CDS             3043..3591
                     /locus_tag="LP452_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014970.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(3884..4831)
                     /locus_tag="LP452_002272"
     CDS             complement(3884..4831)
                     /locus_tag="LP452_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBL2014971.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            5019..6206
                     /locus_tag="LP452_002273"
     CDS             5019..6206
                     /locus_tag="LP452_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBL2014972.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            6187..7491
                     /locus_tag="LP452_002274"
     CDS             6187..7491
                     /locus_tag="LP452_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL2014973.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            7517..8530
                     /gene="citC"
                     /locus_tag="LP452_002275"
     CDS             7517..8530
                     /gene="citC"
                     /locus_tag="LP452_002275"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBL2014974.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            8534..8827
                     /gene="citD"
                     /locus_tag="LP452_002276"
     CDS             8534..8827
                     /gene="citD"
                     /locus_tag="LP452_002276"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBL2014975.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            8832..9737
                     /gene="citE"
                     /locus_tag="LP452_002277"
     CDS             8832..9737
                     /gene="citE"
                     /locus_tag="LP452_002277"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBL2014976.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            9730..11289
                     /gene="citF"
                     /locus_tag="LP452_002278"
     CDS             9730..11289
                     /gene="citF"
                     /locus_tag="LP452_002278"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBL2014977.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            11286..11843
                     /gene="citX"
                     /locus_tag="LP452_002279"
     CDS             11286..11843
                     /gene="citX"
                     /locus_tag="LP452_002279"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBL2014978.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            11836..12699
                     /gene="citG"
                     /locus_tag="LP452_002280"
     CDS             11836..12699
                     /gene="citG"
                     /locus_tag="LP452_002280"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBL2014979.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            12933..13342
                     /gene="tnpA"
                     /locus_tag="LP452_002281"
                     /pseudo
     CDS             12933..13342
                     /gene="tnpA"
                     /locus_tag="LP452_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13979..>14110)
                     /locus_tag="LP452_002282"
     CDS             complement(13979..>14110)
                     /locus_tag="LP452_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2014980.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         5045 a         2103 c         2781 g         4181 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
     1681 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
     1741 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
     1801 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
     1861 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
     1921 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
     1981 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
     2041 atttcaactt ttccttattt atgtactacc ttttacttat taattataag gtagtacata
     2101 tatatctttt ttttggtagt aactaaaaat aaatatgtta taatagtatc aaatgaatat
     2161 tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat atgtaattgg taattttaaa
     2221 ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa
     2281 ggaaaaagaa ccttgataat tgacttggat atgcaaggaa atacttctga tgtgatgaac
     2341 ttaacgcata tgaacttcac taacgaagaa ggaggcggag aaggtgaacc gattgtttac
     2401 gaaaatactg tgactgacgt gttgataaac aatcttccac ctaaagaggc tgtctataaa
     2461 gtaatcaata acctctatat acttccagca gatatgtcct ttgaaatgta tgatgattgg
     2521 atcaaagacc aatataatga ttcattatcc caattcaaat atatgaaaga aaagctagaa
     2581 ccattatttg atgagtttga cgtcatctat ttagatgtcc caccttctat ttctgtatat
     2641 agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg tcttacagac ccaagtaaaa
     2701 tcgatgagaa atgcgatgca atatcttgag tatatggact tttttgtgaa agaatttgat
     2761 acaaatttat atgttgctgg tgttattcct ttcatgttgg aaagcggtga cgcagtagat
     2821 caggaaatgt ataaacaagc ccaagagatt tatggggaac atttgattaa aaatgttgta
     2881 ctcaaaaatg cgcgcttaaa acgatacgac ggatcaggta ttacgatgga gaagacaaag
     2941 aaaggtctct taaaacaatg ggatcgcaaa agccacgaat tatttatcag tattttgaaa
     3001 gaattggaag aacacgagaa ttggtatgcg gaggtggagt aattggctga catcaaagga
     3061 cttgatattt ttgggaacaa caaaggactc gccaaaataa aaaaagaagc agaagcaaag
     3121 aaaaagcagc aagcaattac accatctgaa aataagcaat tagaagtgca ccaagttcaa
     3181 gaaataaaaa cttcctccaa tgttgaaaga cagagtagag gaagaacaaa aaaagttggc
     3241 gcaccaatca gaaagtttga taaggtttat tccaccaagc atcctttaaa actgtcgact
     3301 ttgttaaatg cgacttccag agttttagta gaaaagtatg agactaatat gacaagagat
     3361 gaattgctta gaaaagcttt agatgagtat attaaacaga atcttacgca agaagataag
     3421 caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt tcagagaaaa atatccaatt
     3481 gttccagaat tagatgaccg tggagaaata ataagaacta ccagtcagat aaaacaagat
     3541 accgaagact cactgcgcaa agggtgggga atgaataata aagttagata gaaccccctt
     3601 tttatagcat aaaaaagatt tagaaggata tatgatctgc atgagaagta caataaaatt
     3661 aataagaagt aacattaacg aaaaaacgtg gaaattattc agtgaataca aagaaattaa
     3721 atcttgaaaa gtaatggagt atggcttgga aaagttcaaa aaatatcaag acaagcttct
     3781 caaaaaaagg gattttgata tccaataaaa agagtgaatc attgtactgc cctccaaaaa
     3841 agtagtacca aaaatctaaa ttttttggag gattttacat gactcactct ttttttatca
     3901 aagaaagtat tttccaagca gttttttcat ccaaaaataa atctgtaaaa aaattaccat
     3961 tcaaggcgcc taaacaactc tccgctttga acttactttt acaaatacca attcgttttg
     4021 gaatatcgaa aatttctttt aaagaaagac caaaagtctt atcctcagat gctttgatga
     4081 attcaccatt gatattgaag ggtcttccgt aaagaaatcc aacagtatcc gtcatgcttg
     4141 gaaataggat tttacgatgc ttgttccagg gttcgacaga atcaatagag gataaggtgc
     4201 caattccaga aattaacata tctgcatgcg aagcggttgc taaggtatta gctaaagaag
     4261 gttctagagc cattagttca cgtgcagttt cattgatgac atataaagga atactgagag
     4321 tcaaataaga agactcataa cgttcagcag ctttttgaac taatcgtgtg gagccagcta
     4381 aggaattata ttttccgatt tcgccaatga attgagtaaa aattaattct tcacgagcac
     4441 ccattttaaa ctgatcaatc actttgtaaa cggtatctcc ccaagatagt gcaaccacat
     4501 tggctttctt aattaattct tgaatgtaat cggcagcaaa ttttgaaaat aataaatcgt
     4561 gttgtgataa gtcttcacta ctttttaata tatagatatt cgttttaaag taatttttga
     4621 agatttcttc tagctcataa ttcctagcat acggagaatt aattgaaata ctaactaatt
     4681 gtttttcacg tgcatcttca atatatttta aagctttata tctggtgatc ccatattttt
     4741 tagaaatttc actaatattg atatcctcga gataatagtc ttcacaaatt tttacaagga
     4801 gtgattcata gtcagagaat tccagatgca tagtatagct cctaactttt tcaaatagat
     4861 attttttctc attataccaa aaaaattgaa tttagtcttt ttttaaattt ttaaaaataa
     4921 gcatcatttt tttaaaattt aaaatgaaag ggttttttta ttttaaaaaa agtgttaaag
     4981 tggttttgta attaaaaaat gtaaaaagga gtgaaggtat gcttgatgaa gttttagaaa
     5041 tccataaaaa aaatgtaggt gttttacgga tggaaacaac agaagccgtg caagatgatc
     5101 aagatttagc aaaaatgtac accccaggtg tagcagaact aagtttaatg attgagaaaa
     5161 atcatgcttt ggcaagagaa ttaacaatta gtggtaaatt agtggccgtt attacagacg
     5221 ggtcggcggt attaggtctt ggaaatgtag gaaaccaagc tggattacca atcgtcgaag
     5281 ggaaagcgct gctttataaa aatttagcag gtgtcaatgc gattccatta gccatcgaac
     5341 aaaaatcagt agacgagatt gtacaaacaa ttgtcaattt gcaaaatagt tttgcaggga
     5401 tccatttaga agatattgca gcaccgaagt gttttgagat tgaagaaaaa ttacaagaaa
     5461 aactttctat tcctgtgtat catgacgatc aagaaggaac agcgatagtt gttttagctg
     5521 gattgatcaa tgctgctaag atccaaagga aaacattaac tgaacttcga gttttgatca
     5581 acggcatggg agcatctggg gttgcaacag ctagactatt aatcgctgct ggtatcaaaa
     5641 atctaacatt ggtggataaa caagggagat taaagaagca agatagtaca ttaaataaat
     5701 atcaaagaga gttgttggat catatacatg atacaaataa taatgcaagc gttttggaag
     5761 atgcaatcgt tggacaagat gtttttatcg gtctttcaat aggaaatgtt ttaacgcctg
     5821 aaatgattgg aaaaatgaat gataatccga ttatttttgc tttagccaat ccagtaccag
     5881 agatcacacc cgatttagca aaagaatctg gtgcaaagct aatcgctaca ggttcttcga
     5941 aatatcctaa ccaagtaaac aatattttag tatttcccgg attgttcaag ggactattac
     6001 tatcgaaagc gaataaagtc gatctttcat tacaacttga agtagccaaa caattagctg
     6061 ctaaggtcgc agagcctacg gtagaaaaat tcatccctaa tgtttttgat gaaggagtcg
     6121 caaacgtcgt ttcacaggca gttttggatt atttacaaaa actaaaagaa aacgaggata
     6181 atatacatgc tgaaaacatt aaatagaatt aaaattggtt ttattccttt atctctttat
     6241 atcattttgg tactcgcgta tcttgcaatg acgataagca atatcgttcc agaaaatgat
     6301 atgttagttt ctttgggtat tatgactgtt tttgcttatt tattagaaga aattggtaaa
     6361 actattccta ttttaaaaga tttaggtggt aaagtattag ttgttacatt tttgcctgca
     6421 tttctagtct ataaacattg gctacctcaa agtactgtaa ctgtggtttc tgattttatg
     6481 aataataaca atttcttgat gttctttatc acactactag ttgtcggcag tattgcttca
     6541 atgaatcggc aaacattggt taaagcaact tcacgtatta tcattatttt aattttatgc
     6601 gatgtgattg gttctttgat aggaacaggt gtgggaatac tccttgggct atctgcttgg
     6661 aatgcgtatt tctttatcgt tgcgccgatt atggctggtg gtgttgggga aggagcgatg
     6721 ccattatcga ttggttatgc agcaatatta tctggtatgg gacaaaatga tatctttggg
     6781 aagattctac cgtgtgtgat gttgggaagt ttagtcgcag ttctactgtc tggtatgctc
     6841 aaaaaaattg gagagaaata cccacaatta aatggtcagg gtaatttgat cgatggggat
     6901 gatgaacatt tagagaactt gaagggttca gctaaaccag tcaatattga aaaaacattg
     6961 ttagcaggcg tggtagcaat aacgatttat tttgttgctg tctatgttga gcacttatta
     7021 aaatcactcc ttggcttttc tattccttca cctgtgatct tattagtctc tcttatgggg
     7081 gctaaaatgc ttggttttat ccctaaaaat atcgaagaag gtggaaattc gttgtatgga
     7141 ttcactgttc gtgggatcac acctccctta ctttttggcg taggggttgc aaaaacagat
     7201 tggttagagc tgatcgaagt atttaaaaat cctgcaacga tagtaacaat cattgtgacg
     7261 gtagtagcta ttgtcgctac agcttttatc gccgcaaaat ttttaaaaat gtacccagtt
     7321 gatactgcca tcgcagtttc atgttgtagc ggacaaggcg gtacaggtgc tttagctatc
     7381 ttagctgcgg gtgatcgcat ggaattaatg ccatttgcac aagttgctgt tcgactaggg
     7441 ggtgctatga ccgtaacgtt cgctatcttt ttaatgggtc tgctatcata agaacataaa
     7501 gaaaggacgt gaccatgtgt ttgatcaaac agatatccaa cgattatttt taaaaaataa
     7561 ctatatcaaa gaccaatggg aaaatctttt gcttgattca ggaatacaaa agaagcaaat
     7621 cgaagatttt caacatttag atcaaacatt gggtatatac cataaagaaa agttagtagg
     7681 aacagtatct tatcaaaaca acgtgattaa atatatcgcc gtgtctgaaa aatataaaga
     7741 tggtggacat acatttaatg tactagtctc tgcaataatt caggcaatga gtcaacaaga
     7801 gatttttcat tatattgttt tcacgaaacc gcgctatcaa aaaagtttta actttttagg
     7861 ttttgaaaca atcgtatcta ccccaaaaat agctgtatta gaaatgggag atagttctat
     7921 aaaacagtac ctagagagga ttcctaaagt gaatggtgaa aaaaaagcag caattgtcat
     7981 gaatgcgaac ccatttacag aagggcatta ttatttagta aaacaagctg ccaataacaa
     8041 cgattgggta tatgtatttg tactcgaaaa agaacaaaat cttttttcga cgaaggaccg
     8101 actcaaatta gttcaacaag gattacaaaa actgaaaaat gtcattgttc tatcaggtgg
     8161 cgagtatatt attagccctg caacgtttcc cacctatttt ttacgagaag atgatgaaat
     8221 agcaaaggag caaatggtcg tagatgctac tttattcaaa gaaagaattg ccacttattt
     8281 agatatctct gctagatatg tgggagaaga accactttcc ccaatgactc gttcatataa
     8341 cgacatactg gaaacgattt taccccctga aataaaagtt catgtccttg caaggaagaa
     8401 aacggaacaa caccaagtta tttctgcttc tcaagttcgt aaagcgtatc ttgctgggca
     8461 attggaaaaa ataaaatata tggttccaga aacaacctat caatacttaa aaaataaaag
     8521 ggagcgttaa gtaatggaaa taaataaatc agcgattgct ggaacattag agtcaagtga
     8581 tgtccaaatc atgatttcac cgaatacaga aggaatagaa atcgatttag acagtcaagt
     8641 gattgagcaa tttggaaaac aaatcaaaaa agttatctat gacacactag cagtttatca
     8701 gattaaaaac gtaaaactca aggtagtaga taaaggggca ttagattgcg ttattgttgc
     8761 acgattaaaa acagcgatcc atcgttcctt aggtatccaa caccaagaaa caccgtggga
     8821 ggtttaatta gatgatgaaa gaacgtttga gaagaacaat gatgtttgtt ccaggaaata
     8881 atccagcgat gatcaaagat gcaggtattt atggtgctga ctcgattatg ttcgacctgg
     8941 aagatgcagt gtctatggca gaaaaagaca gcgcaagaat gcttgtggct gaggcattga
     9001 aaacagtgga ttatggtgac acagaattaa ttgttcgaat aaatgacctg cactcagaat
     9061 ttggacgtgc agacattttt gcaatggtta aagccggcgc gcatgtatta cgtttaccta
     9121 aaacagaaac ctctgaagat ataacagaag tagaaaaagt gattatagaa gcagaaaact
     9181 atcataatgt tccgataggg cgaacattga tgatggcagc cattgaaggt gcttctggtg
     9241 tattgaacgc acctgcgatc gccaaagcat ctgatcgatt gattggaatc gccattgggg
     9301 cagaagacta tgtgacaaat atgaaaacca aacgatatcc agatggaaac gaattgtttt
     9361 ttgctcgcag tatgatcgtt catgctgcac gtgcagcagg catttccgca tttgacactg
     9421 tttactctga tgtggatgat atggaaggat ttttaaacga agtacgttta atccatcaat
     9481 taggttttga tggaaaatct tgtatcaatc cacggcaaat tccagtagtc aatactgttt
     9541 atcagccgtc aatcaaagaa atcaaacgat cactagacat catctcggca atcgaagaag
     9601 caaaagaaaa aggttctggc gtaatttctt taaatggaaa aatgatcgat aaaccaattg
     9661 tagaacgtgc acaacggaca ttgatgttag caaaagcatc acgattgatt gatgaggagg
     9721 gaaatatcca tgcataataa acaaagacgt agcattccat caagtgtttt aacaaagtat
     9781 aaaaaagaat tccaaagtgt ttcattatcg ccacaaacga ttcaacgttg tgcaccaaaa
     9841 gtaacgactg taactaatca gacaaataaa ttgatgcact cgatcaaaga agctatagaa
     9901 aaatcgggct taaaagatgg aatgacgatt tcatttcatc atcatttgag agaaggtgat
     9961 tttgttttta accaagtgat ggccgtgatc cgtgaaatgg gcatcaaaga attgacctta
    10021 gcaccaagtt ctttaacgaa cactatgaat gagatggtta ttgaatgcat caaggacggt
    10081 acagtaactc atatcacaag cagtgggatg cgcggcagct taggtgaatt tatttctcat
    10141 ggaggaatgg acaatcctgt gattttacgt tctcatggta atcgtccacg tgcaattgaa
    10201 agtggtgaat tgaaaatcga tgttgcattt ctaggagtac caacatctga tgattatgga
    10261 aatgctaatg cgactgttgg aaaatccgtt tttggttcat tagggtatgc aaaagtagat
    10321 gcacaatatg cagataaagt cattcttcta actgatcatt tagttcctta ccctaatacg
    10381 cccattagta ttccgcaaac acaagtcgac tatgtcgtgg aagtagaagc aattggtgat
    10441 ccgaataaaa tcggtgctgg tgctacacgg tttactaaaa acccaaaaga attaaaaatt
    10501 gctgaaatgg tcaaagatgt cattgttcac tcaccttatt tcaaagatgg cttttctttt
    10561 caaacaggaa cgggtggtgc agctttagca gtaacgcgtt acttagctag tgaaatggaa
    10621 gaaaaacaga tcaaagcttc ttttgcatta ggtggcatca ctaaaccaac ggttgattta
    10681 ttggaaaaag gtcttgttga aaaagtgtta gatgtccaag actttgacaa aggagcagct
    10741 gaaggaatgg cgcggcttga gaaccaacag gagatcgatg cttcctttta tgctgatcca
    10801 agtaataaag gagcggttgt taaccaatta gatgtcgcta ttttatctgc attagaaatt
    10861 gacacaaact tcaatgtaaa tgttatgact ggttcagatg gtgtgctaag aggtgcgatt
    10921 ggtggacatc aagatgcagc aacggctaaa cttactatca tctcagcgcc gttagtacgt
    10981 ggccgaatac ctacagtagt aaatgatgtt actacattga tcacaccagg gaaaagtatc
    11041 gatgtattag tcacagaagt aggggttgct atcaatcccc aacgtaaaga tctaatagct
    11101 atttttgaaa gaattccaca aattcctgtt tttacaatag aagaattaca gcaaaaagca
    11161 gaaaaaatag taggcatccc ggaacctttg caattcacag atcggatagt tgcctatgta
    11221 gaataccgag atggttcaat tttagatgtc gttcgtcagg tgaaagaatt tgaagaagag
    11281 agatcatgat gtttgatact ggagaaattc aagaaatcga ggatattttg atccataaag
    11341 aacaacgtgc taacttgcaa agcaacctgc tttccaagta ttcaaatagt tcattgatgg
    11401 tctttacatt gaatattcct gggccgatca aaaataatcc aatgattgag caggtatttc
    11461 gtcagggaca gcagtcaatt cagcaaatgt ttttaaaaaa aaaggtggaa atgacgtaca
    11521 caaaagaaat caatttgcca agtggattgg actggtacgt aatagtaaca gaagcacctt
    11581 tgaatctcaa aaaatggttg gtcacgttag aagagcaaga cgcattaggt agactttttg
    11641 atttagatgt cttgttcaaa aaaggagagc agataacaat tgtcagtcgc caagctctta
    11701 atcatgggga acgtccttgt tttatttgtg ggaaatcagc aaaggtatgt gcacgtaatc
    11761 gtacacatac tgtgaaagaa atgcaaaaga aaatcgaaca aatagtagcc gattccttaa
    11821 aaggagcatt gaaacatgca tgataatatt caaaaaatag cacaagaagc acaattagcg
    11881 atgctttatg aagttacatg tatcaataaa ccaggattgg ttgatccagt agaccccggg
    11941 tctcatcaag atatggacat ttttactttt ctacaaagta gtgtagtttt gactccctat
    12001 tttgaagaat ttatccaaac aggattggac cgacaacatc aaccaattga agaaaccttt
    12061 atggcgattc gacagattgg tttagaggct gaaactgcga tgttctcagt gacaaatggt
    12121 ataaatacac ataaaggtgc tatcttttca ttggggattt ttcttgctat ttgtggccgg
    12181 ttaactattt ggaaagttcc ttgtaaaaaa agtttctttc aaaaagaaat tcaaaaaatg
    12241 acgaaaaatc tacttaacga ttttgggaag attgatcaaa caaaaccaaa agcatggact
    12301 tggggagaat atctatttgt taaccatgga ttgacaggga tacgagggga agccaaaaga
    12361 ggttatcctt gtgtttttga ccgaggattg ccttattatc aagcatatca agggtcgcaa
    12421 caagataaga tgatagacac tttacttttt ctatcaatgg aagtagaaga taccaatttg
    12481 atcaaacgaa gtaataatag acatgttttt gaagagtatc aattacttat tcgtccttat
    12541 tttgaattag ggggtgtaaa aaccatacaa ggtaaaaaat atttggatta cctaaatcag
    12601 caatttaaaa agaaaaattg gagtattggt ggaagtgcag acttattgat ccttacaatc
    12661 tttttagata aaatcatgga caaaggatgg ttatgttgat actataatac attattttgt
    12721 attagtaagc taataactaa aaaatggttc aatacaaata ttttataaaa aatggtagtg
    12781 cctatccttt cagattgtag acaaagaaaa atttctttgt ctacaatttt tttgttaatg
    12841 acttcagtca gtttcgtgcg caaagcgtgc gaaacaagaa aaccatgcgt ttctacacgt
    12901 ggaagaacta gcatatcgaa aggagttttt tcatgtcaaa tgacgataaa agtttagccc
    12961 atactcgttg gaattttaag tatcatattg tatttacacc aaaatatcga cgaaaagtaa
    13021 tctataggca attacgaaaa gatattggaa aaatacttag gaagttgtgc aaaataaaaa
    13081 acgtggaaat tatagaagca catgcaacgc cagatcatat tcacatgctt gttcaagtac
    13141 catcaaaaat aagtatatcc agttttatag ttatctgaaa gatcgtagtg ctgtgctaat
    13201 ccacgagcag cacgtaaatc acaaatatag atacggaaat aaatcatttt ggtcggagga
    13261 atattatgtg agtacagtcg aattgaatca aaagacaatt gaaaaataca tccgtgaaca
    13321 agaagctgat gatcgcattt gagatagtat aagtaaaaga gagtagatgt tgcaataaca
    13381 aaagggtaaa aatggactac ctccagtaaa ataccgaaaa atagtttaac ttttggagat
    13441 aagtccattt aagggtctat agaatgacaa tttttttagg ggattttact attattactt
    13501 caagacccgc ttggattcgc aaagtgagtg aactcttaaa actacctgat agtaggttgt
    13561 tttaagagtt atctgaaagt aaaaattgca tttatatttt tgataaatac gtaaatttat
    13621 atatacgtat ttatcaaagt tttaggccaa aggaatattg tgtaaataca ctaggattga
    13681 atgaatagac cagaagaaat tgcgtccaag aacaaaagaa gcatgatagt gctctatata
    13741 aattgggcat tcaagaatct gaaatttaaa ggtagaattt tagttagact tttagatttt
    13801 aaacacaaaa ataaccatac aagcaacaag aagcaaagtg ctatattata aattgtccgt
    13861 ccgtttactt acataagatc attggggcta tttttaaaag agaataatag ataataaatg
    13921 aaaaaaaagt caagtccaca atcctgtgta aaatactata caatgctctc actatttttt
    13981 acttggcaaa tttaatatat tttcttgatg aagaaagcca ctcatcgtgt tgatcaataa
    14041 ggactgctcc aattaaacga ttggctgaaa cgcagtttgg aaagatacga ataatttttt
    14101 ctcttctgcg
//
LOCUS       DAFELO010000069        13869 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFELO010000069
VERSION     DAFELO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.072"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP452_002283"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP452_002283"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL2014981.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LP452_002284"
     CDS             complement(2260..2511)
                     /locus_tag="LP452_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL2014982.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LP452_002285"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LP452_002285"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL2014983.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="LP452_002286"
     CDS             3461..3679
                     /locus_tag="LP452_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2014984.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="LP452_002287"
     CDS             complement(3968..4480)
                     /locus_tag="LP452_002287"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL2014985.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LP452_002288"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LP452_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL2014986.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="LP452_002289"
     CDS             complement(6933..7376)
                     /locus_tag="LP452_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL2014987.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="LP452_002290"
     CDS             complement(7377..8165)
                     /locus_tag="LP452_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2014988.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="LP452_002291"
     CDS             complement(8162..9103)
                     /locus_tag="LP452_002291"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL2014989.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="LP452_002292"
     CDS             complement(9280..9867)
                     /locus_tag="LP452_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL2014990.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LP452_002293"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LP452_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL2014991.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="LP452_002294"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="LP452_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL2014992.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="LP452_002295"
     CDS             complement(12784..13719)
                     /locus_tag="LP452_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2014993.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3835 a         3102 c         2445 g         4487 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtat
//
LOCUS       DAFELO010000070        13803 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFELO010000070
VERSION     DAFELO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.073"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            191..709
                     /locus_tag="LP452_002296"
     CDS             191..709
                     /locus_tag="LP452_002296"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2014994.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="LP452_002297"
     CDS             837..1520
                     /locus_tag="LP452_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL2014995.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="LP452_002298"
     tRNA            1829..1902
                     /locus_tag="LP452_002298"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="LP452_002299"
     CDS             2061..2420
                     /locus_tag="LP452_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL2014996.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="LP452_002300"
     CDS             2543..4174
                     /locus_tag="LP452_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL2014997.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="LP452_002301"
     CDS             complement(4236..4877)
                     /locus_tag="LP452_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL2014998.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5063..5139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="LP452_002302"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="LP452_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL2014999.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="LP452_002303"
     CDS             5494..5838
                     /locus_tag="LP452_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL2015000.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="LP452_002304"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="LP452_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL2015001.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6237..7385)
                     /locus_tag="LP452_002305"
     CDS             complement(6237..7385)
                     /locus_tag="LP452_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2015002.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7397..7708)
                     /locus_tag="LP452_002306"
     CDS             complement(7397..7708)
                     /locus_tag="LP452_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015003.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7761..8156)
                     /locus_tag="LP452_002307"
     CDS             complement(7761..8156)
                     /locus_tag="LP452_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL2015004.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8192..8536)
                     /locus_tag="LP452_002308"
     CDS             complement(8192..8536)
                     /locus_tag="LP452_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015005.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8837..9058
                     /locus_tag="LP452_002309"
     CDS             8837..9058
                     /locus_tag="LP452_002309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015006.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9185..9961
                     /locus_tag="LP452_002310"
     CDS             9185..9961
                     /locus_tag="LP452_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL2015007.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9964..10152
                     /locus_tag="LP452_002311"
     CDS             9964..10152
                     /locus_tag="LP452_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015008.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            10154..10438
                     /locus_tag="LP452_002312"
     CDS             10154..10438
                     /locus_tag="LP452_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015009.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10459..10647
                     /locus_tag="LP452_002313"
     CDS             10459..10647
                     /locus_tag="LP452_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015010.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10973..11212
                     /locus_tag="LP452_002314"
     CDS             10973..11212
                     /locus_tag="LP452_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015011.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            11218..11904
                     /locus_tag="LP452_002315"
     CDS             11218..11904
                     /locus_tag="LP452_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015012.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKKMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11907..12737
                     /locus_tag="LP452_002316"
     CDS             11907..12737
                     /locus_tag="LP452_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBL2015013.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            12754..13605
                     /locus_tag="LP452_002317"
     CDS             12754..13605
                     /locus_tag="LP452_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2015014.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13602..>13803
                     /locus_tag="LP452_002318"
     CDS             13602..>13803
                     /locus_tag="LP452_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL2015015.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDF"
BASE COUNT         4610 a         2237 c         2704 g         4252 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaataaata aactgctcta tctcttgtgt agcaagggat
     6181 ggagcttttt tgtaaattat gggatttttt ttgaccatac ttttgaccat actttcttag
     6241 aagttaatat aatttgttag tttttgtgtt gtttcttctt ttttgctgtt agtaatgtgt
     6301 gtatatgtgt ccatagtagt ttttatattt gcgtggccca ttctttcttg gacttctttg
     6361 tgatctgcgc ctgcctcata tagcattgat gcatgtgtat gtctaaaacc atgcagaccg
     6421 atataaggta agccggcacg ctcacgatat atattgtatc tttctgccac tgactgatta
     6481 attgtgaaat tattttcgtc attggtaaac aaataattag ctttagtaaa tcctaattga
     6541 aataagtact ttctttgttc gagcttccaa aattttagtt gctttatagt tttttcatct
     6601 aaataaatta ttcgatttga ttttttggtt ttaggagttt cagatatata atatttatct
     6661 gaacgagcta cagttttttt aatggccatt tcatttgttt taaaattaat attgtcccaa
     6721 gtgagtgcta atatttcgcc gattctacat ccgctgaatg ctaatagtct aaagagcgta
     6781 tagtctctgt ttttaaaata tggattctgt tcttgagata cttcatttag aaataattcc
     6841 aactgttctt ttgtatataa ttttaatttc ttttctgttt taatttgtgg ctttggaata
     6901 agtaaattta atgttggatt atcagatgtt aaaccaatat ttatagcata cttaaaaact
     6961 ttattcatat agtttatgaa aagagggtac tgctttgaag ttcctttttc actccaatca
     7021 tttactacct tttgacaata tgccgtatta attttagaaa gcttaatatt tccaaatttg
     7081 ggatatatat gtttattaaa gattatttta gtccttgaat atgaactttc ttttacagtt
     7141 tttttgtagc tttctagcca taattctgct gcttctttga aagtagtatt tgtacttgtg
     7201 ggcattcctg ttttttgtaa ttctagttct aacctagaaa gtgctgtttt tgcttctttt
     7261 tgtgttctaa atcctcgttt agtagtatat tttttctttc cagttagcgg atcaataccg
     7321 agataagttt taaagtacca tgctttttcg ccatttttct ttttatattg ttttatcatt
     7381 gccatcaata ttacccctat tcaaattcta attctgccat gctatcattg aaaaattcga
     7441 tcagtccttg ttcctctata aattcaagcc atatttcctt cccgttaatt aatggaactc
     7501 ctttttcttt acgaattgtt tgatacaagt tagtcacgac ttgtttccac atttctaata
     7561 cagctttatc tattttctta tcatcccagc gttttttttg gggtctattt ttgttttttt
     7621 caatttgata attagtagct ttgatgataa atgtttcgta tccggttgtg cttttttcta
     7681 accattcttt gaattctgta tatatcattt ttgttcacct ttctattttt gaacgtatgt
     7741 tcttttttgc ttaaaaaatt ttagagagct gcataacaat agatgcgagc ttttaataat
     7801 tctttcacaa gtggttcgaa gcgaacgctt aaattgttat cttctataaa tttcatgtaa
     7861 ttgatattgt gtggttctaa ttcagacttt gcaatatatc tatctaataa tttttcaatc
     7921 atgaagcgat cagcctcata ttccattttt gaatggagag agaatgctaa gttatataaa
     7981 aaataattat catgatgttt agaagcgtgt cctaattcat gtaataaggt tctttgtctt
     8041 ctatacttag ataaattagc ttttattact atggtattca tacaagcaac ataatggcca
     8101 tcggcatcaa ggttttcacg ctcttctacc ttaacgccga gttcattaat tatcatttca
     8161 atttgactgt ccaaatgact cacctactta tttagttatc ttttttctct aaatatgctt
     8221 caatcatagc tgatagtatt tccctatcat tatcggtgag cggttttccg tcgctactca
     8281 taacagatgc taaagcttct tccacggtta gttttctttc ctctggtgca actcccgcgt
     8341 taggatcatc tgtgcgtcct agcaaataat ctgttgatac atggaagtag tcagcaactt
     8401 tttgcaaagt ttctatttta ggactttgtg tcttccattt ataaatagag ttttttccaa
     8461 atcctattct attttctaat tcagaaatag aaatacctct ttttttacag agctctttaa
     8521 ccctttcgaa aagcatgtca aatcaacctt cctagagcgt acgataataa tttatacaaa
     8581 aagatagaaa ggtagttgac aaatctatcc aattgtctta tatttaactc gtaagctaat
     8641 ttaataagct aacaagcaac aagaaataca cctaataaaa taaacgaatt cgaggtcggc
     8701 aaactaagaa tgttaatttt tagggttttt ctaggcttat ttaactgtat ataaatataa
     8761 gacagttgga tagaaatgtc aataaaaatt agctaatttt attagcttac atatttaaag
     8821 aaaaggagga aacatcatga aacaaaataa aaaaactcct tccgcaaaag gagctttaat
     8881 aactttaaat tttaaagacc aaaagtttag tgctgttgtt gatgaaattc atccacaaga
     8941 gcatcaaaaa aattcttttg caactgacga gaaaaagact cagaaacgct ctgagaaagt
     9001 tcttcgaata attcaatttc tgttggaaaa gattcgtcat tttggaagtc tattttgaat
     9061 tctttattaa cagtcttgaa tacggtttgt ttgaataagg cgtcgacatc catattaaat
     9121 tcttccatac tattttcacc tcgctttcta ttcaaaatta tatcaaagaa aggaatgagc
     9181 aaaaatgaac acaccgcaaa tttttaactt tgaacaacat gaagtaagaa cagtaacaat
     9241 acatgatgaa ccattttttg ttggaaaaga tgtggctaaa gttctagggt accaaaatgg
     9301 tagtagagat attaacagac acgtcgatgt agaagatcgg caaaactacc aaaacggtac
     9361 ttttgaatcg cctagagggt taactattat taacgaatca ggtttgtact ccttaatttt
     9421 aggaagcaaa caaccaaatg ctaagaaatt taaacgttgg gtgactagcg aagtgttgcc
     9481 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
     9541 tccagataca atcatccaat tagcaactaa gttgaaagaa gaacgtactg gaagactaat
     9601 cgcagaacaa aaaattgcag aatacgaacc caaaatttcg tatttggata gtatcttatc
     9661 ttctacagat tcagtaacta ttagtcaaat cgcggctgat tatgggatgt ctccacaaca
     9721 gatgaacaaa ctacttcata agctaggtat tcagaaaaaa gtaggcaacc aatggttatt
     9781 atgcaaaaaa cacatgagac agggttacac aaaatctcat acaactgaga ttccgaaatc
     9841 tgatggcgga acaaaagttg tgatgaatac aaaatggact caaaaagggc gtctgttcat
     9901 ttatgagtcg ctgaaaaaag aaggatatat tccagaaatc gatttattag aggaggaatg
     9961 acgatgtcac aagaatttat tttgaaagtc agaattcaat tagcaaaata cggaaagtct
    10021 cagaactggt tagctgatac tatcggaatt tctagaccat atatgtcgga cattatgaat
    10081 gggcgtagaa aaccagataa acaaatcaag ccaattgagg cagcactagc agagttagag
    10141 aaggagaaat aacatgcaaa taacaattcc agataattta gtagtttccg aattaactac
    10201 acagattacg aatgctgttc ttaattcatt ggacgaacga ttacacctta tgaacaaatc
    10261 agtagagctt cctccatatc caaacaaatc agaggtaaaa aaagttttag gcattggtga
    10321 tgacaaatta acacattgga taagcctagg cttaaaaaca cagcagtgga gcaagttaga
    10381 catcagaatt gaacgatcgg aactccaaag atttttgaaa gaaaactttg agttctaaag
    10441 gcaaaggaga atgattttat gtcctacaca ttgcaacaag aacatcaaat tctaggtctg
    10501 attaaacagc gtagaaaaca attacaagat gatcgtgcag cgcttagaaa atccgatgag
    10561 ctatcagata gacaagctga actaattgct tctgaacttg aggatttgag aatgctagaa
    10621 ataaaaaata gggagattag attatgaaga agacagacac actttttata ggattcattt
    10681 tggggttatt agtgattgta gcgcaccaaa gtattatcgg aggaagcttg ttcgcagcat
    10741 tgatggtttt aatcaatctg cttgactcaa aagaaaggag caactatggc acgagaagaa
    10801 gcgctaaaaa tcggtaaagt gattgctgat aattggtggg caaatagccg ttctattatt
    10861 ttaagcaagc aacatatcga aaagaaaaaa gcatggcaac aaataaaaaa gtgactccgc
    10921 cgaccaaagc aatgagtcac tcaaaaaatc ataactaagg agattttaac atatggaaaa
    10981 tgaactttcc actctagatc aatatttggc cgatcctgat tggggcaaat cgaatttaca
    11041 actggttgct aacaagtacg aaaaatcaat tgatgaagca attgttgcat tgactgatgc
    11101 agcttccata accgctgatg acagtaaatg gaccaagaga gacttgggca ttctaaaacg
    11161 aggcgttaat tggcttgaag atcagtacag agaagaaaca aaagagaagt gatatgagtg
    11221 tttaaaccac taatagattc atactcggcg gtactaaaga aatttaaagg taacgacatt
    11281 ggtgcaacaa tcaacgaaga agtaaatatc gaacggctga aaaagatgta tgacggatat
    11341 gatggcgatc gagtcattga aattcgcttt attgatccac gacggttcac agtacagcaa
    11401 agaaacttca tctatgcgct cataggcgat attttcatcg atacaggcat gccaacggac
    11461 ttctggaagg aattcttcta cttccgtttt gaaggtgtca cagggcgcga aataagcctc
    11521 aaagacgaat cgaatacaac cgtgagtgat gccaatatct tagcgaatat catcctagat
    11581 ttcatctttg aacatcatat tcctttcaaa gaaggatatg agattttacc agcgaatcaa
    11641 gagtattact tctacaaatg cattacaaaa agagtctgct gcatctgtgg caaaacagga
    11701 gctgatatcg atcactttga caaagcgctg ggaagacgaa agcgcaaaga agttgatcat
    11761 tcagagtaca catttgcagc actctgcaga atccatcaca cagagaaaca caaaataggt
    11821 gtgatcaatt tcaaaaataa atatcaaatc aaagggatca agttaaacca ggaaacaatt
    11881 aaaaagttaa ggataggagg ataaatttgg ctgagataag ttggatcaaa cttaaaacta
    11941 ctatgtttga cgatgaaaaa atacgattaa tccaagctgt tcctgagtcg gatgccatca
    12001 tcgttatatg gattcgatta ctagttttag caggaaagac taacgacgat ggtctgatat
    12061 atatccagag gaacatgcct tataccgaag aaatgcttgc tacattgttt ggcaaaaacg
    12121 taaatacggt tcgcttagcg ctaactacat tggcaaattt caacatgatt gatctaagca
    12181 gtgatggact aattgccatc agtaattggg aaaaacatca aaatatcgag ggtatggata
    12241 aagtaaggct aaaaaatgct gaaagaaacc gtaaatacag ggaaagaaag agacaggaac
    12301 gtctcaaatt ggaaaatgac gttagcgtga cgtcacgtga cggtacagat aaagatatag
    12361 aagaagataa agatatagat aaagaagaaa agaaaggtaa gtattctgac gaacacttac
    12421 gccttgctaa aaagttgcaa agtaatttaa ctgaagattt tccaaaagaa atgaacaaag
    12481 tagatatcga aaaatgggca gacacaatca ggttgatgga agaaagagat aaagcatcta
    12541 tagaagcgat tgagtatgtg atcaattggc tacctacaaa tgaattttgg tttggaaata
    12601 ttagaagtgc taagaaattg agagaaaaat ttgagaagct caaattcgaa atcaaagcag
    12661 acaagaagaa tcataaaaag caaagtcaaa aactacagta cagcaatcct agtgaatatg
    12721 acgacttgcc aatttaaaaa ggagatgcat cacatggaaa gcctagcaaa tgctatggag
    12781 aaactaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg
    12841 tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac atgcatgagt
    12901 tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat atataacgat
    12961 agcttaaaag cacgagcgtt gagttttttt caaaatggtt cggtattaac agataaaact
    13021 ttgtttaaat gcaaaatgga aaattatcac gtagtggatc aagaaacaaa aatcgcttta
    13081 gaaaaagcaa aaagctatac gaatgaggtt ctgctgaacc atcctgcaca tttcattcta
    13141 tcagggaaat caggaagcgg aaaaagccac ttatcaatgg ccacagcttg ggaaatactt
    13201 gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagaatt attagagcaa
    13261 ataaagtttt cttataacaa tactgaactg agaaaagaaa ttgaaggatc gcttatagcc
    13321 gatattaaaa caactgattt ggtggttttt gacgatattg gagctgaatt aggtagtggg
    13381 gtatcaaata gtaggcagtt tacaaacaac acgttaaaca cgctcttaga agccagacag
    13441 aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga agcctacggt
    13501 gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa attccaacaa
    13561 acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa taccgtaata
    13621 aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag tattacgatc
    13681 tagccttgtg gcaagctgaa gcaaatggct ggaaagtaaa acttcaggaa agatttgagc
    13741 tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat attcccgact
    13801 tca
//
LOCUS       DAFELO010000071        13787 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFELO010000071
VERSION     DAFELO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.074"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            46..2211
                     /gene="acm"
                     /locus_tag="LP452_002319"
     CDS             46..2211
                     /gene="acm"
                     /locus_tag="LP452_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL2015016.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2483..2968)
                     /locus_tag="LP452_002320"
     CDS             complement(2483..2968)
                     /locus_tag="LP452_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL2015017.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3058..3403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3448..4089
                     /locus_tag="LP452_002321"
     CDS             3448..4089
                     /locus_tag="LP452_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2015018.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLSVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4108..4743
                     /locus_tag="LP452_002322"
     CDS             4108..4743
                     /locus_tag="LP452_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2015019.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4761..5576
                     /locus_tag="LP452_002323"
     CDS             4761..5576
                     /locus_tag="LP452_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2015020.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5895..7331
                     /locus_tag="LP452_002324"
     CDS             5895..7331
                     /locus_tag="LP452_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2015021.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7520..9967
                     /locus_tag="LP452_002325"
     CDS             7520..9967
                     /locus_tag="LP452_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL2015022.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10197..11264
                     /gene="rlmN"
                     /locus_tag="LP452_002326"
     CDS             10197..11264
                     /gene="rlmN"
                     /locus_tag="LP452_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL2015023.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11457..12560)
                     /locus_tag="LP452_002327"
     CDS             complement(11457..12560)
                     /locus_tag="LP452_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2015024.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13113..13661
                     /gene="aac(6')"
                     /locus_tag="LP452_002328"
     CDS             13113..13661
                     /gene="aac(6')"
                     /locus_tag="LP452_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL2015025.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4720 a         2298 c         2917 g         3852 t
ORIGIN      
        1 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
       61 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      121 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      181 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      241 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      301 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      361 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      421 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      481 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      541 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      601 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      661 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      721 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      781 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      841 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      901 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
      961 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1021 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1081 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1141 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1201 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1261 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1321 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1381 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1441 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1501 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1561 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1621 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1681 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1741 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1801 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1861 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1921 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     1981 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2041 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2101 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2161 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat
     2221 aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa
     2281 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
     2341 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
     2401 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
     2461 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
     2521 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
     2581 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
     2641 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
     2701 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
     2761 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
     2821 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
     2881 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
     2941 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
     3001 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
     3061 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
     3121 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
     3181 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
     3241 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     3301 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     3361 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     3421 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     3481 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     3541 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     3601 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     3661 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     3721 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     3781 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     3841 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     3901 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     3961 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taagcgtggc aatcatctat
     4021 cttacattga ctggtattct aacaatcgta atgaaacaag ttgaaggaaa attaaattat
     4081 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     4141 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     4201 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     4261 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     4321 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     4381 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     4441 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     4501 ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg
     4561 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     4621 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     4681 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     4741 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     4801 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     4861 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     4921 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     4981 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     5041 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     5101 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     5161 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     5221 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     5281 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     5341 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     5401 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     5461 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     5521 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     5581 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     5641 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     5701 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     5761 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacatatttt
     5821 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     5881 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     5941 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     6001 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     6061 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     6121 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     6181 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     6241 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     6301 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     6361 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     6421 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     6481 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     6541 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     6601 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     6661 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     6721 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     6781 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     6841 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     6901 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     6961 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     7021 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     7081 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     7141 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     7201 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     7261 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     7321 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     7381 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     7441 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     7501 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     7561 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     7621 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     7681 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     7741 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     7801 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     7861 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     7921 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     7981 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     8041 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     8101 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     8161 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     8221 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     8281 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     8341 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     8401 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     8461 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     8521 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     8581 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     8641 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     8701 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     8761 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     8821 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     8881 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     8941 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     9001 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     9061 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     9121 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     9181 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     9241 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     9301 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     9361 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     9421 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     9481 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     9541 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     9601 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     9661 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     9721 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     9781 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     9841 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     9901 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     9961 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
    10021 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
    10081 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
    10141 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
    10201 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
    10261 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
    10321 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
    10381 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
    10441 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
    10501 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
    10561 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
    10621 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
    10681 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
    10741 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
    10801 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
    10861 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
    10921 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
    10981 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
    11041 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
    11101 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
    11161 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
    11221 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
    11281 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
    11341 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
    11401 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
    11461 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
    11521 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
    11581 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
    11641 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
    11701 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
    11761 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
    11821 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
    11881 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
    11941 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
    12001 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
    12061 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
    12121 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
    12181 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
    12241 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    12301 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    12361 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    12421 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    12481 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    12541 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    12601 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    12661 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    12721 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    12781 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    12841 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    12901 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    12961 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    13021 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    13081 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    13141 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    13201 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    13261 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    13321 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    13381 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    13441 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    13501 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    13561 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    13621 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    13681 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg
    13741 aaattccaac ttatttccga agaagctact tttggaacac cgtgtac
//
LOCUS       DAFELO010000072        13488 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFELO010000072
VERSION     DAFELO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.075"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(164..388)
                     /locus_tag="LP452_002329"
     CDS             complement(164..388)
                     /locus_tag="LP452_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL2015026.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="LP452_002330"
     CDS             complement(586..2166)
                     /locus_tag="LP452_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL2015027.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="LP452_002331"
     CDS             complement(2567..3943)
                     /locus_tag="LP452_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL2015028.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="LP452_002332"
     CDS             complement(4071..5189)
                     /locus_tag="LP452_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2015029.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="LP452_002333"
     CDS             5331..5765
                     /locus_tag="LP452_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2015030.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="LP452_002334"
     CDS             complement(5843..6376)
                     /locus_tag="LP452_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL2015031.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LP452_002335"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LP452_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL2015032.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LP452_002336"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LP452_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL2015033.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="LP452_002337"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="LP452_002337"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL2015034.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="LP452_002338"
     CDS             complement(10101..10679)
                     /locus_tag="LP452_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL2015035.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="LP452_002339"
     CDS             11001..11471
                     /locus_tag="LP452_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL2015036.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="LP452_002340"
     CDS             complement(11579..12805)
                     /locus_tag="LP452_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2015037.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3959 a         2720 c         2248 g         4561 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat acccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaa
//
LOCUS       DAFELO010000073        13427 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFELO010000073
VERSION     DAFELO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.076"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..912)
                     /locus_tag="LP452_002341"
     CDS             complement(<1..912)
                     /locus_tag="LP452_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL2015038.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="LP452_002342"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="LP452_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL2015039.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2004..2174
                     /locus_tag="LP452_002343"
     CDS             2004..2174
                     /locus_tag="LP452_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015040.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="LP452_002344"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="LP452_002344"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL2015041.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="LP452_002345"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="LP452_002345"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL2015042.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="LP452_002346"
     CDS             4438..5319
                     /locus_tag="LP452_002346"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL2015043.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="LP452_002347"
     CDS             5316..6032
                     /locus_tag="LP452_002347"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL2015044.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="LP452_002348"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="LP452_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL2015045.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="LP452_002349"
     CDS             6749..7750
                     /locus_tag="LP452_002349"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL2015046.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="LP452_002350"
     CDS             7747..9108
                     /locus_tag="LP452_002350"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL2015047.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="LP452_002351"
     CDS             9068..10219
                     /locus_tag="LP452_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL2015048.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="LP452_002352"
     CDS             10194..11363
                     /locus_tag="LP452_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2015049.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="LP452_002353"
     CDS             11538..12917
                     /locus_tag="LP452_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2015050.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="LP452_002354"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="LP452_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4483 a         2339 c         2923 g         3682 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gagaaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DAFELO010000074        13346 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFELO010000074
VERSION     DAFELO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.077"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(45..1364)
                     /locus_tag="LP452_002355"
     CDS             complement(45..1364)
                     /locus_tag="LP452_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2015051.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LP452_002356"
     CDS             complement(1361..2014)
                     /locus_tag="LP452_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2015052.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LP452_002357"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LP452_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LP452_002358"
     CDS             complement(2724..3440)
                     /locus_tag="LP452_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL2015053.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="LP452_002359"
     CDS             complement(3564..3749)
                     /locus_tag="LP452_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015054.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="LP452_002360"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="LP452_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="LP452_002361"
     CDS             complement(5356..6387)
                     /locus_tag="LP452_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015055.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="LP452_002362"
     CDS             complement(6394..7224)
                     /locus_tag="LP452_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015056.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="LP452_002363"
     CDS             complement(7221..8036)
                     /locus_tag="LP452_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2015057.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="LP452_002364"
     CDS             complement(8033..8857)
                     /locus_tag="LP452_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL2015058.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="LP452_002365"
     CDS             complement(8847..9947)
                     /locus_tag="LP452_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2015059.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="LP452_002366"
     CDS             10125..10910
                     /locus_tag="LP452_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL2015060.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="LP452_002367"
     CDS             10943..11326
                     /locus_tag="LP452_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL2015061.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="LP452_002368"
     CDS             11402..11533
                     /locus_tag="LP452_002368"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2015062.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="LP452_002369"
     CDS             11502..11738
                     /locus_tag="LP452_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2015063.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="LP452_002370"
     CDS             complement(11816..12787)
                     /locus_tag="LP452_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL2015064.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4055 a         2911 c         2306 g         4074 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacgtcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atgtcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DAFELO010000075        13241 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFELO010000075
VERSION     DAFELO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.078"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(94..2193)
                     /locus_tag="LP452_002371"
     CDS             complement(94..2193)
                     /locus_tag="LP452_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL2015065.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(2413..3522)
                     /gene="rpoD"
                     /locus_tag="LP452_002372"
     CDS             complement(2413..3522)
                     /gene="rpoD"
                     /locus_tag="LP452_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL2015066.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRHLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(3546..5387)
                     /locus_tag="LP452_002373"
     CDS             complement(3546..5387)
                     /locus_tag="LP452_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL2015067.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(5569..7884)
                     /locus_tag="LP452_002374"
     CDS             complement(5569..7884)
                     /locus_tag="LP452_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2015068.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(8069..8929)
                     /locus_tag="LP452_002375"
     CDS             complement(8069..8929)
                     /locus_tag="LP452_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL2015069.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(8988..9509)
                     /locus_tag="LP452_002376"
     CDS             complement(8988..9509)
                     /locus_tag="LP452_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL2015070.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            9787..10548
                     /locus_tag="LP452_002377"
     CDS             9787..10548
                     /locus_tag="LP452_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2015071.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            10574..13165
                     /gene="mprF"
                     /locus_tag="LP452_002378"
     CDS             10574..13165
                     /gene="mprF"
                     /locus_tag="LP452_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL2015072.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSAFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
BASE COUNT         3573 a         2759 c         2333 g         4576 t
ORIGIN      
        1 aaaaaggagt atgacaacag ttgtgtcata ctcccctttt tcgaataaac ggtgttaaac
       61 ggcaggctca cagcctcttt catttgccta aaattaattg ctcccatttc ctccgaacca
      121 tccagtttta cagttgatgg ttacgatgta aaatggcaaa gcactttctc caatgccttt
      181 catcaatgca gattttgttg aagtggcata tagattataa tagccttccc cattttcgat
      241 tctggctttt tctaatctgt aacctccttc aacgatatat ccacgttgga tacatgtagc
      301 aatcactttg ctttggacac ctggtgccca cgtccatgct ggattagatg catcggcaat
      361 atcacttgct ctggcagatt tgattggctg attcgctgag tttgaagagg aaggggtcga
      421 ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg cttgagctgc
      481 agctgctgct tgagcggctt gttttgcttg ttcttcagct gcttttttcg cttcttctgc
      541 ttttttcttt tcctcttctt cttttttctc tttatcagtg agcatcttat ctacttgttt
      601 caattgttca ttcaactgag cagatagatc tgtatttttc actttttcta ctgcttcttt
      661 tgccgtcttg tattggtcgc gtgttgctga atccactact tttccgtctt taaagactat
      721 agctgttttt tctttagctg tttgcaattc agcatattga tttttagctt ctgtttgtgc
      781 ttgttgaatc aattttccaa aatctgtgtt atccgtttcg attgcttgga tctctttatc
      841 tgctttcaac ttgatgtcat ctgctagatg atcatcgacg atggcaggtg ctatgaacca
      901 ctcattcact ttttggatat ctcggatctt agattggata tcttggtaag ttgtttctaa
      961 ctctgaatat cctttttcat tcttaagact ggataaatca ttttttagtt ttgataaatc
     1021 ttgatgaatc atatctgcac gaataaattg atggtgatca tctgtataaa aggcagcaag
     1081 tttctcatga agttcttcca tttgttgctg tctttttgct tctgctgcta cttcggcttc
     1141 tactttttga tgatggtcat aataagccca gcttcctcca ccgagtagaa gtactgctgc
     1201 taaaacgaga taaggtactt tttttgattt tttcttttgc gctttatctg gatctgtttt
     1261 ttctaagttt gattcttgat gtgttttttc agtatctggc acgattgtta ttgatttcgc
     1321 ttctgcttca aacagcttag gctggttctc ttctttttct atttttgctt caagatcagt
     1381 accctctttg cttacagatt gattttcagc atcggattca ctatttattt catcgggctc
     1441 tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg gcagtttatt
     1501 cgttttttcc ttttctaatt gtttttcagt aaatacttca gttttttctg tgatatccgt
     1561 ttttacgtct tttattttta ctgtttcttt acctgtggcg ttttctctat cagagtcttt
     1621 ttcagtttct tgttcctttt ctggctctac tttgttttcc tgtgcaattt tttcactgat
     1681 cattttgagc agctctttcg cgtgctcttc ttttgtatct tcacgatgtg ctttgatata
     1741 agcggatagt atcgaatttt cttcctcatt atcgacgatt tctttcagtt cagctactct
     1801 tcttttttgt tttgattcaa gcgtttcttc ttgattcttt cctgattctt ccgaagtctc
     1861 atgaacagtt gctttcttct gttcattttt ctctatatta gttacttgat cgtgagcagg
     1921 tggttcttcg tcttctggtt ctgctttttc gaataattcc attacagatt ctagaggtaa
     1981 atctttcaat tccgaccatt cgattggatc gtttaattct gtatcaggaa agactgcatc
     2041 ttcagttgat ttatcctgca tagctgcatc tgtatttgtt tcttcaattg attcttttat
     2101 ttgctgatct tctgcagtta tttgatgacc gcagtctgga cagatagtat tctgctccat
     2161 ttttttcgat ccgcaatttg gacatttttt catcacttag cgatcggctc ctttactagg
     2221 tttacgctac catttaatct atcatattct atacgtcacg ccaatagctt atttttagga
     2281 tttacttaaa aataatgaat gaatttcctt cctatagaaa aaaaagcttg agactgcgta
     2341 gtctcaagct ttttttcttt taaaataaac caaacagttt tactgatttc tcttctggtc
     2401 aatagtccac gcttattcta agaaatcttt taattgtttt gaacgagaag gatggcgcaa
     2461 ttttcttaat gctttagcct cgatctgacg aatacgttcc cgagtaacac caaatacttt
     2521 ccctacttct tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac
     2581 attctcttcc cgatcagtca atgtatctag tacatcttcc aattgttctt ttagtaactc
     2641 ataagctgcg tgttctgctg gacttgttgc ttcctggtct tctatgaaat cacctaagtg
     2701 agagtcatct tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa
     2761 gatttctcgg actttttctg ttggaagatc catttccgca ccgatttctt ctggcgtagg
     2821 ttctcttcca aggtcttgca gcaaatgacg ttggatacgt atcaatttat taattgtttc
     2881 aaccatatga actggaatac ggatcgtacg tgcttggtca gcgattgctc gagtgatggc
     2941 ttgacgaatc caccatgtag cataggttga aaacttaaat cctttgcggt agtcaaattt
     3001 ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga actgcatgcc
     3061 tcgtcctaca taacgtttag cgatacttac tactaaacga aggtttgctt ctgccagacg
     3121 ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct aacgctactt cttcttccgc
     3181 agttaaaagg gagacacggc caatttcttt cagatacata cgaaccgggt cattgatttt
     3241 tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt tctcagcact
     3301 cttcaagctg tgaattgatg gatcaccatt ttcatccacc acactgatac ctgcgtcttc
     3361 aatcttttgg atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg
     3421 attggataaa tcatcatata caacttgtcg tttgggtttg ttttctttga tgaaagctgc
     3481 tactgctgct tcatacgttt ttttatctgt tccatttgcc ataaaagaag gccccccttg
     3541 agtagctatt tggcttgttt taactgtttc gttaaattga tgatttcaat cgtcaattca
     3601 agctcaagct gtttgtttcc agttcgtctg gcttcttgct gcatttgttt ttttaatgtt
     3661 atttcatcag ctaagccaga tttgctgatc acatctagca agtcttggat ttctcgtggc
     3721 gaactttctt ctgccaccgg taaatattcg atttccacca ccaaccgcct taaattttca
     3781 tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga gacatatgca
     3841 tcgaataaaa aataaacttc tgcatatgca tcatgaataa actgaaattc tcgttctttt
     3901 aaaatattac ggacagaaac ttctttaaat gcacgatata atagaatctg ctctgccttt
     3961 tctacttgcg tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt
     4021 tgactttcct gaggttcttg acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt
     4081 ttttgtatcg tctctctgcc gatctgaaac tctgttgaca gttgagatag atacatatct
     4141 tgttcgattg gtgaaacaac tcggctaagt tcaactagta attcttcgag gtattcgatc
     4201 ttgtcttttt cattatctaa atttttccct tgttttaaat aacgtgattt aaaagtaaag
     4261 actgtttcgc gcccatgagc cactaattct tggaaagatg cttctccata cttacggaga
     4321 tattcatcag gatcaagctt ttctggaacg actacgacag aaagtgctaa tcgactgttt
     4381 tgccgaagca attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag
     4441 caaaagacca attctttcgt cagcttttcc atttgttgga tctgttgagc tgttagactt
     4501 gtccccatag aagctaatcc agttttgata ccagcctgcc atgcagcaat cacatccata
     4561 aacccttcga acaacaaaat ttctccgttt ttccggatat cgcttcttgc tttatcaaga
     4621 ttaaataaaa cttgtctttt attgaacagt tctgtttctg gactattcaa gtatttgggc
     4681 tgatcttttt cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg
     4741 ccttgagaat tacgaagtgg aaacatcact ctttgataaa aacgatcaga catacggcca
     4801 tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt catttgaaat tgattcattc
     4861 ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt gaattcttca
     4921 attaattcta aagacaaccc acgactttgc aaatattcta aagctggctg tccagctttt
     4981 gtatgcaata gcatgtgatg atagatttcc gctgcttttt catgagcagc aatcagtttc
     5041 cccgtagcag attcaggatt ttggacagct atagtacgct gacgccattg atcttctaac
     5101 ggtatctgtt cgaagtctgc cacttttacc actgcttctg gaaaacttag accttctagt
     5161 tcttgaagaa aagtaaacac gtttccgcct ttaccacaac caaagcaatg aaaaatctgc
     5221 ttgtcttctg caacagaaaa agacggtgta cgttcttcat gaaaaggaca cagccccaag
     5281 taatttttac cagacttttt caactggaca tattgtccaa tcacctcaac gatattcgtt
     5341 tgactgcgta ctgtttcgat aacctcttga ggaatccgtt gagccatttt ttcaccccct
     5401 gctctattga tatcttttgg gtttaacaga agatatttca ggagaataac cggtaaaaaa
     5461 tcgcactaac tgaaatcagt gcgattttaa aggacacaat tatacatgtt tgattgtatc
     5521 atatttcatt gctttttcaa gtataacaca tttctatctc attaagcatc aatttgctac
     5581 tcttttcagt aactgatttt gaatatcagc cttccagtac aatactcgaa gaacaatatt
     5641 tacgagcatg aatacaaaga agtatcctac gctctctccc aaccctaagt ttacttccaa
     5701 aattccatgg aacagcattg cagctacata tatccctacg atcgtcgtta ctgctaggaa
     5761 gatgcgcaat gggttgaagg gataacaagc ttttacaact gccatgcaac tgatcccaat
     5821 cagcaagtaa tacatcaaag tagtagtttc agcatttccc catgcgaatg tttttccaag
     5881 tagatacacc gcaatgatat tcactactac aagcaaggca tttggtaaag cattaataag
     5941 tgctgtaggc aaataacggt aagtgatttt tcttttatct tgttcaaaag ataaaaagaa
     6001 ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt tggataggga taaacggaaa
     6061 tccaattgct gtaacggcac atataatcga taaaataaat gaataaatcg ttttgataaa
     6121 aaagatgcct gagactttcg tcacattgtt gaccactcgg cgtccttcaa acaggacttc
     6181 tggcaatgta gtaaaatcag agtccaacaa taccagattc gcaatctgac gtgttgctcc
     6241 atcaccttct gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa catcattcac
     6301 accgtcgcct gtcattgcaa ccgttctgcc gctttctttc aattcattta ccagcaattt
     6361 cttttgctgc ggactcactc gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc
     6421 catttcttca gtaatttgag aaagatcgac atacgcatca taattcggta atcctgcacg
     6481 tctagcgata ttggagacag taaaaggatt gtctccagaa atcactttca gatccacacc
     6541 ttcctcatga agataagcta gtgtttcttg agcgtttttt cggattggat catcgatttc
     6601 aaacagagca accggctcaa caaatgctgg tttttcttcg ttcaattgct gatttggagc
     6661 gatccccaac ataagtacgc gatagccatt cgcttgggct gtttttagac tgtccggaag
     6721 ccgatcttct tccaccaaac gttcaggcgc tcctacgtac acgactccca gttctggaaa
     6781 ttccatcgcg ccccatttgc gatcagaaga aaatgcgact ttgtttgtca catcataccg
     6841 attagaaagc tcaaaataat ctctcatagc ccgcatagtg atgttgttat cactgctttc
     6901 tgccagatac gtccccataa tttctgggat ggctttctcg tacagcggat gtagtgcttc
     6961 tacccgctgg atactcattt tcccttcagt aatggtccct gttttatcca agcataatgt
     7021 atcgacatgg gccagcgttt caattgaata catgtcttga acaaggattc gtttttttgc
     7081 cagtttggtt acccctgtcg ttaatgcaat acttattagc aataccaaac cctttggaag
     7141 catgcctaac aaagctgctg cagatgcaat aacagaaatt ttgatagaag catcgcggat
     7201 gaacagtgct tccaaaaata gtatgatccc caaaggaata atcacgaaac tagtgaattt
     7261 ggatactttg cgaatcgaat ctacaagttc agaacgaata gctttgtgag tctttgcctc
     7321 gctagtcaag cggacagcat aattatctgc acctactcgg ataacttcag ccatgacaga
     7381 accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca gtttctttga caatcaaatc
     7441 agattcgcct gtcaaaagag cttcatttgc ttctgcccgt ccagatataa cctgtaaatc
     7501 ggctggtatc tgttctcctg ctccgatttt taccaaatct cctaatacca gctctttagt
     7561 agaaatcgat tgttctttac cgtcccggat cactagtact tctgattggg aaacaatcga
     7621 taatttccgt acaagatttc ttgcatgtat ttcttgataa attccaataa cgatattgac
     7681 gacaataatt gctaaatacg ccatgttgga atacgcacct acaaataaca gacaaatccc
     7741 aataccaaag ttcagaaaat tgaataacgt caatatatta tcgctgaata tttgttttgt
     7801 actttttgat gcatcttcct ggtagtcatt ttgctggcca gaagccattt tctgaactac
     7861 ctcttgctgt gtaagtccat tcattttctt tttctccatt ttccgccaac tttcaaaaat
     7921 aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac aacttgtttt tataaaattt
     7981 atagaaaaac acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc
     8041 gtgtgctgtt tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc
     8101 accaacctag tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc
     8161 ccaatcccgc gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa
     8221 tgaaatggaa tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc
     8281 caagaacaca aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt
     8341 taaatccgtc agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca
     8401 atcctgggac gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat
     8461 tctgatcgac ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa
     8521 caaaagtaac aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt
     8581 tccataacgc aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt
     8641 catacgcacc taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc
     8701 ctagaccaac aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta
     8761 aaaaagaaat cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc
     8821 ctggcaagat aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt
     8881 tggttgtgta ccccacttct tgattttcca ttttttgcct tttttccact atttgcgcac
     8941 tccttctttc atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact
     9001 ttttcatagg tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta
     9061 cctccattat gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg
     9121 tattgtttcc cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct
     9181 gctaattcta ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca
     9241 aaattatatg cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc
     9301 atttccgcca aatatttgac accttgatca atactgtcag ctggattatc aatgatctca
     9361 gcttctccat gcagactttc cgagctctgc atgagatctg tcccttttcc ttttgattcg
     9421 gtcaatatta tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct
     9481 gttgttattt ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcat
     9541 aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata
     9601 gataatcgtt ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact
     9661 atttctttat cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg
     9721 aattttcctg tattttttga catcttacga aaatgatcgt tgtttttcag taaaaacaat
     9781 tgcccaatga ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa
     9841 aagaaatatg caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc
     9901 ttccctctct ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc
     9961 cctgtactcg aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg
    10021 acaaacgaag catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg
    10081 ttgctggaaa aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca
    10141 ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac
    10201 acgcttgtta aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg
    10261 gtatggttac tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt
    10321 ttgctgcatg acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg
    10381 atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact
    10441 ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat
    10501 cctgaacgct tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata
    10561 gaaggtgatt tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt
    10621 caaaacaatc tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg
    10681 taaaactcta tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag
    10741 cggactaatg ctattgattg gtcttatctc tgtgttacct atggttggtt atgatattat
    10801 tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga caagctggct
    10861 gatcaatacg atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc
    10921 tgaactatat ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt
    10981 cttgtttttg atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt
    11041 cacgccagtt gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata
    11101 ttttcctgtc gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc
    11161 acttaaaact caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt
    11221 tagtttcctt tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc
    11281 attattcatt gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag
    11341 tttcgatgta atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt
    11401 tgtgtggatc ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt
    11461 ctttttcttc aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca
    11521 attagcaaca gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat
    11581 gctagtactt tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt
    11641 gaacccattg aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt
    11701 aatcattatg ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt
    11761 cttgattgtt ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt
    11821 tttcttgtct attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca
    11881 attgatctat tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt
    11941 gctttacatc gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt
    12001 tatttccttt ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat
    12061 cgctgttagt ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat
    12121 tggcgaagcc tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc
    12181 cgatagtcaa ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga
    12241 accaactgtt cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc
    12301 ttctggtaag aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg
    12361 actatgctat ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga
    12421 attcggctac gatttcatta agatggggga agaagcttat gtagacctga atagttttac
    12481 aacttcaggg aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg
    12541 atttacattt gatgtcttac aaccaccatt ttctactgag caaatgagtg ctttcaaaaa
    12601 tatctctgat aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc
    12661 tgaagattat ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt
    12721 cgcttttgca acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat
    12781 gcgatatgat tctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt
    12841 tgcctatatg aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa
    12901 tgtcggaact tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt
    12961 tggttctcat ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa
    13021 ctgggtctca cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc
    13081 tttgctgatt attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa
    13141 aaaatggata cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg
    13201 aataaaagag actgaagtaa aacgaaagtt catgcttcta c
//
LOCUS       DAFELO010000076        13112 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFELO010000076
VERSION     DAFELO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.079"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(68..1747)
                     /locus_tag="LP452_002379"
     CDS             complement(68..1747)
                     /locus_tag="LP452_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL2015073.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            2295..3797
                     /locus_tag="LP452_002380"
     CDS             2295..3797
                     /locus_tag="LP452_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015074.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNLKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3810..4472
                     /locus_tag="LP452_002381"
     CDS             3810..4472
                     /locus_tag="LP452_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015075.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4573..4776)
                     /locus_tag="LP452_002382"
     CDS             complement(4573..4776)
                     /locus_tag="LP452_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL2015076.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(4887..6071)
                     /gene="iadA"
                     /locus_tag="LP452_002383"
     CDS             complement(4887..6071)
                     /gene="iadA"
                     /locus_tag="LP452_002383"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL2015077.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(6228..7967)
                     /gene="yfcC"
                     /locus_tag="LP452_002384"
     CDS             complement(6228..7967)
                     /gene="yfcC"
                     /locus_tag="LP452_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL2015078.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(8740..9354)
                     /locus_tag="LP452_002385"
     CDS             complement(8740..9354)
                     /locus_tag="LP452_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2015079.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(9960..10337)
                     /locus_tag="LP452_002386"
     CDS             complement(9960..10337)
                     /locus_tag="LP452_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015080.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(10637..11518)
                     /gene="galU"
                     /locus_tag="LP452_002387"
     CDS             complement(10637..11518)
                     /gene="galU"
                     /locus_tag="LP452_002387"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL2015081.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(11561..12580)
                     /locus_tag="LP452_002388"
     CDS             complement(11561..12580)
                     /locus_tag="LP452_002388"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL2015082.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3889 a         2771 c         2207 g         4245 t
ORIGIN      
        1 tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt catcttactc
       61 tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc aagatcactg
      121 ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg tcttgcttta
      181 atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt tcaatcatat
      241 tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc cgtgaagtaa
      301 tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc gctacgaaaa
      361 tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata tcacctatgc
      421 ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc atacgcttgt
      481 tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat ggcatcccta
      541 ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga actggcacaa
      601 aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg gcatggccag
      661 aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa atgatatctt
      721 ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata taaaccaaat
      781 ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc agagacttga
      841 tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca tagtttttca
      901 tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct aaacgcatcg
      961 ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt tgtgcttttt
     1021 ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa gcgacgatga
     1081 tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg gcttcagaag
     1141 ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag actccttctt
     1201 tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc gtttggtcaa
     1261 atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg atccccactg
     1321 aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca aagtctattt
     1381 ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca ttacgactga
     1441 cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca gaaatttttg
     1501 acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa aagacaccag
     1561 ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg tcgatcccca
     1621 acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc tcgcccactt
     1681 ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata attttaatgg
     1741 tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga ttttcttctt
     1801 tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt ctcaacttat
     1861 ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat gggcatttta
     1921 ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc cagaatactt
     1981 atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc gctagtatca
     2041 gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg atttcttttg
     2101 ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat tttaccacat
     2161 tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga tggtgtgcaa
     2221 acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg catctactag
     2281 gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa gtaaaatggg
     2341 gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg ctagctggcc
     2401 tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa gttggagcaa
     2461 cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa acttcttcaa
     2521 gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca ccagtttcct
     2581 taacggacgc ggaaaaaata gctgaattag ataatttaaa gagttattcc tttgaaagtt
     2641 ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat tcaagtgaag
     2701 acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc gggaatccgc
     2761 aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa aatagtaatt
     2821 tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc gacaaaggca
     2881 caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca gtcggagata
     2941 ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg atcaaaggaa
     3001 tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc atgaatccgt
     3061 cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca tctgacgata
     3121 acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat gagtttctca
     3181 aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat gattccatgt
     3241 atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat gttgttttac
     3301 tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc attcgggagc
     3361 gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa atcattctcc
     3421 aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct tcgcttagcg
     3481 gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca gaaaccacga
     3541 cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa gaacaaggcg
     3601 gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct gatcaagtca
     3661 gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga gtagcatttg
     3721 gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta aacccaagaa
     3781 aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg ggattttggt
     3841 atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa gctggaaaaa
     3901 tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg cttatttctg
     3961 gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg aacaaaatcg
     4021 ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac aacttgcttc
     4081 cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag accaagcaag
     4141 agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat gaaatattag
     4201 caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct atcgttcgtg
     4261 ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat ctagatgaaa
     4321 caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa aatcgctgta
     4381 ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc tttgaactaa
     4441 aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca aataaaaaat
     4501 ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga ttcaattagt
     4561 aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga cttcgtcgcc
     4621 taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc cttctgtttc
     4681 tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga ctactttatc
     4741 tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc ttagtttgtt
     4801 tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa cgctcgtttt
     4861 ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc ccatactttg
     4921 acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat atcaagcgtt
     4981 tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc ttttttatcc
     5041 aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc caaagggata
     5101 ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact tcctactcct
     5161 aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc atctgaggtc
     5221 atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc tttgctgaag
     5281 cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt gaagtcaatc
     5341 gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt gcgatttgca
     5401 tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg tgtcaatagc
     5461 tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt tccggataac
     5521 atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact tggctgagaa
     5581 gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat tgtcaaaaag
     5641 tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc ttcgtataca
     5701 taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc agtcatatct
     5761 ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc tgtagtcaat
     5821 tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc tccacctaaa
     5881 atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc tgaaagttct
     5941 tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa aatcttccca
     6001 ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc aatttgtcga
     6061 atgattttca tacatttttc ctctttcttt aagtataact gatgatagga tcttaaaaaa
     6121 taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg gttcttggag
     6181 tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta ctgcagacct
     6241 attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc ttgcatcttg
     6301 atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac accaatcaag
     6361 ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata agcaagaact
     6421 gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg catagtcaaa
     6481 gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa gatgtacatc
     6541 gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc atgcaaaatc
     6601 gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag aatccctttg
     6661 gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg gaaagaagaa
     6721 gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat tccagcaact
     6781 aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt ttgagtcacg
     6841 ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt atgcccaagc
     6901 atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata atcatctgat
     6961 ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat catcatataa
     7021 ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc accagacaac
     7081 acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat ccaagaggtc
     7141 gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga gtcatatcct
     7201 aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc tgccatcccg
     7261 aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa acgctccagt
     7321 cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt acgcataata
     7381 ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc agatccatac
     7441 ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc gccaaaatca
     7501 tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata gatattttcg
     7561 ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt ttcctgcgta
     7621 agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac atccagtttt
     7681 tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata cgacttatcc
     7741 agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact tgtttcgcca
     7801 tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac gacaaaagtc
     7861 agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg tgtcgataat
     7921 ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt tatttcttcc
     7981 tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat tatacagtaa
     8041 aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt ttgttttttt
     8101 tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt atttctctgt
     8161 ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct gtttttttca
     8221 ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca cttataaata
     8281 tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt
     8341 ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat
     8401 tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag
     8461 aatccatatg gattctttct tgtctctttt tccactactt tttatttttc tgacactaca
     8521 tattcttata actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa
     8581 caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt
     8641 cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga
     8701 acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg
     8761 gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga
     8821 ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt
     8881 ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg
     8941 cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc
     9001 tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag
     9061 aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag
     9121 cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt
     9181 ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct
     9241 accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc
     9301 tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct
     9361 ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata
     9421 aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc
     9481 attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa
     9541 aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc
     9601 actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct
     9661 gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa
     9721 tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat
     9781 gatttatata tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat
     9841 tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca attaaataaa
     9901 tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc tactaaatat
     9961 taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga aaaagtagtt
    10021 gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt
    10081 agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat
    10141 tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt
    10201 tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca
    10261 aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca
    10321 gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa ttcgcctttt
    10381 tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca ttttctttta
    10441 tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat tcacgaaagt
    10501 ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa ctaaaacgac
    10561 agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc attctcagca
    10621 ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg ataatatatg
    10681 cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt gtcttcatat
    10741 atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct
    10801 ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct gctcctggtt
    10861 cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg ataatggcta
    10921 aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact ctgcaacgac
    10981 ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca ttttctggta
    11041 cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc aattgttgag
    11101 tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac ggttcatttc
    11161 ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga gattgacgga
    11221 tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct gttttttcct
    11281 tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca atgggtcttt
    11341 tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct tcttccacaa
    11401 taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg gcttttgttg
    11461 ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg accttcactt
    11521 gctgttcctc ctaaacgacg attaactatt gatcatctat ttaacgaaga gaaaattcat
    11581 tctccatttt tccgtcacgt aacatgattt cttttgctgc ttttttgata tcttctccat
    11641 tatacaaaac attatagatt gtttgagtaa ttggcatctc aacacctagc tgttgagcta
    11701 gctctactgc tgcttttgtc gttgccacac cctcaacgac cattcccatg ttttctagaa
    11761 cttcttccag cttatgtccc tgtcctaaca agttaccggc acgccaattg cgagaatgaa
    11821 cacttgtaca agtaacgatc aagtccccca caccactcaa accgataaat gtcagaggat
    11881 tggcacccat cgcgacacca agacggctga tttctgccaa tcctcgagtc atgattgctg
    11941 cttttgcatc atctccgaat cctaatccat ggatagcgcc ggcaccaatt gcaatgatat
    12001 ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt atttgtataa atcctgaaat
    12061 aatcattcat gaacagtttt tgtacatagg ctgctgcttt ttgatcaata cttgcagctg
    12121 taattgttgt gatatcatgt actgccactt cctcagcatg gctaggacca gataatacaa
    12181 caattgcctg tcttttttct tctggaattt cttcagccaa aacttctgag attcgtttat
    12241 gcgtatcttg ttctagtcct ttacttgcat gtataatgat tggcttagta ttcattttac
    12301 caataagttc ctgtgcgact gaacgaatcg cttttgttgg aacaacaaat aaaatagcat
    12361 ctgcgttatc cactgcttct gccaattctt tatatccgat aatcgattct gggatcgtca
    12421 aatcaggtaa aaaatgctga tttgtatgat acttattgat ttcatcaatc tgttcaggta
    12481 catttcccca aatacggaca tcatgcccgt tttctgccaa tgtttgtgct aaagcagtgc
    12541 cccaagatcc aggaccaaga actgcgattt tttgtttcat ggatattcct ccaaataaat
    12601 aattgataat ttccattata gtatacattt cttaaagttg ggactaattt ccattgtttt
    12661 taagtgtttg tttacttctt ttatcgtttt tacttgaaat aatcaatgat atgttacaat
    12721 agtctacatt ttatttttac actcgttatg ctttgcttgt tcctaagagg aagcagtttt
    12781 gacaatgtta ctaccagcat tactattagg aggttaggaa acttctccta gtgagtattg
    12841 atttaaaata cataagagac agattgaaca tttctaaatc tgtcaggtcc tgcaacggac
    12901 agattgtctc tgttgacggt tgacatagga acttttgttt tagtcaacat tctggataca
    12961 aaacgctatg taaactggcg caactcgttg tacttggact tttactacgg atccatgcaa
    13021 caactaaata gtactattgc ataacaaaaa atggttggca cccacatgtc ccattaaaca
    13081 gattataagt tgaactgtag ataatgcaaa aa
//
LOCUS       DAFELO010000077        13049 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFELO010000077
VERSION     DAFELO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.080"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(77..149)
                     /locus_tag="LP452_002389"
     tRNA            complement(77..149)
                     /locus_tag="LP452_002389"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="LP452_002390"
     CDS             530..1099
                     /locus_tag="LP452_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL2015083.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="LP452_002391"
     CDS             1086..1946
                     /locus_tag="LP452_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2015084.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="LP452_002392"
     CDS             2136..2840
                     /locus_tag="LP452_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2015085.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="LP452_002393"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="LP452_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL2015086.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="LP452_002394"
     CDS             4677..5993
                     /locus_tag="LP452_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015087.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="LP452_002395"
     CDS             5994..6863
                     /locus_tag="LP452_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL2015088.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="LP452_002396"
     CDS             6918..7727
                     /locus_tag="LP452_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2015089.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="LP452_002397"
     CDS             complement(7830..8459)
                     /locus_tag="LP452_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL2015090.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="LP452_002398"
     CDS             8629..9921
                     /locus_tag="LP452_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2015091.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="LP452_002399"
     CDS             complement(9997..10659)
                     /locus_tag="LP452_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2015092.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="LP452_002400"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="LP452_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL2015093.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="LP452_002401"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="LP452_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL2015094.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGVETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4337 a         2254 c         2825 g         3633 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaaggggt
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttctt
//
LOCUS       DAFELO010000078        12596 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFELO010000078
VERSION     DAFELO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.081"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(9..158)
                     /locus_tag="LP452_002402"
     CDS             complement(9..158)
                     /locus_tag="LP452_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015095.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            554..754
                     /locus_tag="LP452_002403"
     CDS             554..754
                     /locus_tag="LP452_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2015096.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1322..1885
                     /locus_tag="LP452_002404"
     CDS             1322..1885
                     /locus_tag="LP452_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015097.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1946..2290)
                     /locus_tag="LP452_002405"
     CDS             complement(1946..2290)
                     /locus_tag="LP452_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015098.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2387..2632)
                     /locus_tag="LP452_002406"
     CDS             complement(2387..2632)
                     /locus_tag="LP452_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015099.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2702..2956)
                     /locus_tag="LP452_002407"
     CDS             complement(2702..2956)
                     /locus_tag="LP452_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015100.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3120..3443)
                     /locus_tag="LP452_002408"
     CDS             complement(3120..3443)
                     /locus_tag="LP452_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015101.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3630..3703
                     /locus_tag="LP452_002409"
     tRNA            3630..3703
                     /locus_tag="LP452_002409"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3664..3666,aa:Ile,seq:cat)
     gene            complement(4088..4585)
                     /locus_tag="LP452_002410"
     CDS             complement(4088..4585)
                     /locus_tag="LP452_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2015102.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4625..5434)
                     /locus_tag="LP452_002411"
     CDS             complement(4625..5434)
                     /locus_tag="LP452_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2015103.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5768..6625
                     /locus_tag="LP452_002412"
     CDS             5768..6625
                     /locus_tag="LP452_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2015104.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6618..7520
                     /locus_tag="LP452_002413"
     CDS             6618..7520
                     /locus_tag="LP452_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2015105.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7553..7903
                     /locus_tag="LP452_002414"
     CDS             7553..7903
                     /locus_tag="LP452_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2015106.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7952..8875
                     /locus_tag="LP452_002415"
     CDS             7952..8875
                     /locus_tag="LP452_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2015107.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8953..9846
                     /locus_tag="LP452_002416"
     CDS             8953..9846
                     /locus_tag="LP452_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2015108.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9884..10447
                     /gene="amaP"
                     /locus_tag="LP452_002417"
     CDS             9884..10447
                     /gene="amaP"
                     /locus_tag="LP452_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL2015109.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10461..10649
                     /locus_tag="LP452_002418"
     CDS             10461..10649
                     /locus_tag="LP452_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL2015110.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10662..11564
                     /locus_tag="LP452_002419"
     CDS             10662..11564
                     /locus_tag="LP452_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2015111.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11596..11838
                     /locus_tag="LP452_002420"
     CDS             11596..11838
                     /locus_tag="LP452_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2015112.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12094..12465)
                     /locus_tag="LP452_002421"
     CDS             complement(12094..12465)
                     /locus_tag="LP452_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015113.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4461 a         2113 c         2366 g         3656 t
ORIGIN      
        1 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
       61 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
      121 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
      181 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
      241 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
      301 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
      361 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
      421 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
      481 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
      541 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
      601 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
      661 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
      721 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
      781 cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata
      841 gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata
      901 aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa
      961 tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat
     1021 taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag
     1081 taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg
     1141 aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag
     1201 aatgagatct aataaaacct gatctttcat ctgttttttt aataacgtaa taattttttt
     1261 attttttgtg ctttcgccgt tattgtagag tgatttagca ttcttatttt taaaaataac
     1321 tgtgttatat attaaaaaag gcataatagt tataagcttt tttaaagaat atttagaaat
     1381 tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct attttcaggg
     1441 ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag caatggcagg
     1501 ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag ccacgacaga
     1561 ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac gatgcatggg
     1621 aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag
     1681 caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat cgtatacaga
     1741 cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt ctagggaagt
     1801 tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg aagataaccc
     1861 taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat gaagtaagag
     1921 gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc tccaaatttg
     1981 ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg ttgcgctcta
     2041 cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga tttttccact
     2101 ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga tgatattgga
     2161 ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc tttccccagc
     2221 catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga cgaggaagct
     2281 ttgaaatcaa tttttcatgc cataatcaca tagaaatgtc tcctttatat atagattgtt
     2341 aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc
     2401 ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag tcagcgatat
     2461 agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga tatttgaata
     2521 aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca gttcttaaga
     2581 aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc atttaatcac
     2641 ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca atcaatagta
     2701 ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa gacaaaacca
     2761 gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt
     2821 tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt tctttgttcc
     2881 aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc
     2941 tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag ataacatgtt
     3001 attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat
     3061 gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc
     3121 tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg
     3181 atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca
     3241 aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga
     3301 agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc tgaggtttga
     3361 taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat cgcaataaat
     3421 ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt ttctaaaata
     3481 agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa taagaataac
     3541 aatattattt tacttatatt ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa
     3601 aagtaacaaa aataaatttt atttatgatg tacctttagc tcagttggtt agagcagacg
     3661 gctcataacc gtccggtcgt aggttcgagt cctacaaggt acataagagg ttgtgaaaaa
     3721 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt
     3781 ggctgttctt attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa
     3841 atcagcgttt tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc
     3901 cgtttgttaa gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg
     3961 agcctgccgt ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt
     4021 aggactaaaa cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata
     4081 gatattttta tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt
     4141 ctccgtgttt tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag
     4201 cactctgcag gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt
     4261 cgatctcttt ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt
     4321 caggtggaat cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggagaaagaa
     4381 gagggatcaa ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat
     4441 cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac
     4501 gttctgccaa ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat
     4561 tttgcatatc aataataact aacattttct ttcctcctac attcatatcg tcattttatt
     4621 ctttttaata gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta
     4681 cttggtttgc aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc
     4741 tgtaattcaa catatcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt
     4801 cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg
     4861 tgatttctaa gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc
     4921 tttgtagctt ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa
     4981 gagaaggatc ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat
     5041 gttcataata agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt
     5101 gggtttctcg gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc
     5161 tcagtgtatc gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca
     5221 tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa
     5281 aaatatcttt agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt
     5341 catcaaattt ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca
     5401 aggtaccgtc taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat
     5461 atctattttc ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct
     5521 ttgtcattat gaattttttc aaaaaacatt cttaagaaaa aggtggctat atatagtagt
     5581 ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg
     5641 cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta
     5701 aaaccaaatg aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt
     5761 gattttgatg gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta
     5821 tgctccttac ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg
     5881 cataaaattt tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga
     5941 aacggtaaat aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat
     6001 ctacgaagac ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt
     6061 accaaactta ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt
     6121 agtactagac gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc
     6181 tattatcgat ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt
     6241 gtacttcaat tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac
     6301 agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga
     6361 tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca
     6421 gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa
     6481 agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac
     6541 gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac
     6601 gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa
     6661 aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta
     6721 aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac
     6781 gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag
     6841 aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg
     6901 cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca
     6961 tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag
     7021 cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat
     7081 caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa
     7141 atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg
     7201 accaagatgc attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga
     7261 atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg
     7321 aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt
     7381 ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg
     7441 ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa
     7501 cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag gaatggaaat
     7561 tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat
     7621 cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa
     7681 aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat
     7741 cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg
     7801 attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt
     7861 actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc
     7921 aaaaaggagg tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac
     7981 agatgaaaaa aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca
     8041 agcaatcgaa gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga
     8101 agtttctcgg atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt
     8161 gattaatcta gatcagtcag ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt
     8221 actttccgat aatctcttga ttacttgtat cgatagaaaa accgaatttg tggatcggct
     8281 gctgacaaag catggaaatc atttaaactc gtttgaaaag atcatcacgt atacattatt
     8341 agatatttac acccattacg tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt
     8401 ggataaagca gctcgtaaaa caacggaaaa tgaagaactg tataaacagg cagatcttga
     8461 aagagacatc gtatatatcg atcatacctt aagagatcaa cgggaaacaa tggatcaatt
     8521 atggaattct gaaactttca tcaaacgttt agacgatgaa cagctactct atgatgtcca
     8581 tttgagacag cgacaaacag aaaaaatgat cgagatctat cgagatttat tagagagtat
     8641 tggtgatcta ttcaacggga tgatggacaa taatctaaat catatcatga aatatctaga
     8701 ttcagctgca ttaatcattt ccgttccggc gttgttcgca gggatttggg gaatgaatac
     8761 aggaggatta ccgggtaaaa gttctgctct aggctttata ctcgtagtct ttggctcttt
     8821 gatagcagca gtagttttag gttaccattt ataccggaaa gattatacaa attaaagaat
     8881 ctatttaaaa caggtatcaa acaaagaaat ttgttttggc aatacaattt aaaaactagg
     8941 aggaatacag caatgacaga accaaaatta atcgacccgc gaaacttgta ccatacggag
     9001 aaatttccta aacaagatca ggatacacca gctttacagc agaatatgat tcctgttcct
     9061 gattgtggag aagaatccta tgaaggcaat aatcaactag aaaaccgccg tgtactaatc
     9121 actggtggag attcagggat cggtcgtgca gcagcgattg catttgctag agaaggggca
     9181 gatatcgctc ttcaattctt cccaggagaa gaaaaagatg cagaggaagt agcaaaatac
     9241 gtgaaagttg caggacgtaa gattgtctta ctaccttttg atcttcgtga cgaacaagca
     9301 cctaaagaaa tcatagataa agccgtgagt gaacttggcg ggctagatac gatggtacta
     9361 aacgcaggac agcaaatttc ttgtcagtct attgctgagt taccacttga gcaagtcaaa
     9421 gacacattta tgataaacat catcgctatg tttgctttag tgaaagaagc tgttccgcat
     9481 atcccagcag gaggagcaat cgtaacaaca acttctgttc aagcattcaa tccaagcgaa
     9541 catttattag attacgcagc aacaaaagct tcaatcgcta actttacgat cggtttagca
     9601 aaacagcttg cacctaaagg aatccgtgta aatggtgtag cacctggtcc aatctggaca
     9661 ccattacaac tagacgaagg acaaccttct gattcgatcc ctgaatttgg tcagcaaagt
     9721 ttactagaac gtgcaggtca gccaggagaa ttagcaccag tttatgtttt cttagcatcg
     9781 aataaagcaa gttatgttac agcccaaatt tacggagtaa ctggtggaga agcgatcaac
     9841 ttataagaag ttgatcgttt cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa
     9901 aacgatcgga gtgatcggaa cattactttt gctatctgtg cttttgtttg cgatactatt
     9961 aagtgcaacc tatccattgc cttttcagct agagaagttt cgattctttt ccatcactaa
    10021 ttcatacgtg caacaatatg tattttggct tgccgttgca tttgctattt tgacaattat
    10081 cacgttactt gttttcctgt tttatccaaa aacaatcgga aactttctac tgaaacatgg
    10141 tgatggggag ttgacgttgg ataaaaaagc aattgaagga atggttcgtt ctcacttgca
    10201 tgaagatgaa ttcatccatt caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc
    10261 cgtcaatgta aaaggggagt tgaaacgaac ttcagcactc attggtaaaa caggaacatt
    10321 gatgaaagaa atcgaaaatg aagtacaaca catattggga acaaaagagc cagtgaaagt
    10381 atcggtgaaa tacacaggct ttgaagaacc agctaaacga agcaatgacc gcaaacgagt
    10441 agaatagggg gggaccaaaa atgaaagaac tattttcgac atacaagctc cctatcatct
    10501 ttggggtact tggattgata ttagccgttt tatggatcac cgtgggcttt ttcaagacct
    10561 tattgatctt gatcatggca gcgatcggcg tcgctatcgg tttttatctg aaacaaacga
    10621 aaatcttaga tcactatttt aaatcttagg aggaaacatc catgaataac aataacaaaa
    10681 ataataacaa taaaatgaac aaccaaaaac cagtgtctgc acatccagac ccaacgacac
    10741 caaaccaatc aaatggtgtg aacgatgaaa agaacaaaac agcaccagta tctgcacatc
    10801 cagatccagt agtccctaac gttcaacaag gagtagaaga gaagaaacat gatgcagaaa
    10861 agcatgcacc tcatcatcca ggaactccag aatctaaagt tccagaacaa catgatgcaa
    10921 tcgatccttc taaacctgtg gaagagcaaa aacatccttc tgaccatgcg aaagcacaag
    10981 aaaaacttag ttctgaccac acaaacggac aaggaaaact tggttctgat catacaaata
    11041 accaaggaaa gcatgattct gaacaggaag attcaatgcg cggtgagctg aattatgaag
    11101 ataaagttgt gcaaaaaatc atcggaatcg caattgaaca agtagacggt ttattatctg
    11161 caaacggtgg tttcttctct aatgtggcag gaaaacttgt gaatacagat aatgtaacgg
    11221 ctgggatcga aacagaagtc ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg
    11281 aatatggcaa agatatcgaa aaaatctttg aagaaatgca agaagtaatc ggcaaagaag
    11341 taaaaaatat gacacattta gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa
    11401 caaaagaaga atttgaaaaa gatcaagaaa cggtgcaaga caaagtaact gacgcagcga
    11461 aaaaaaccgg tgaatttgct tcgaatcaaa cagataaagt aaaatcagca gctggaagcg
    11521 gcgcacaaaa agtaaaagaa aatacagaac cacgggttca gtaataagga agtaaaacat
    11581 taggaggaac gaaacatggg atttatttgg tcacttatcg tcggaggaat tatcggagca
    11641 attgcaggag caatcacgaa taaaggttct tcgatgggga ttattgcaaa tgtcgtagca
    11701 ggtttagttg gatcagcact tggtcaagca ttattaggtt cttggggacc aagtattgca
    11761 ggaatggcaa tcgtgccatc attgattggt gccatcattt tagtactaat cgtatcattc
    11821 tttgctcgaa aactgtaaaa agacttttaa aaagaggtcg tgaaaaagct gttcttcatc
    11881 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttgggcatat
    11941 tatcgaagat gcaagctttt gaacactgtt tatacggaat aagaggctgt gacaagactt
    12001 ttgtcacagc ctcttattta tgcgggcaaa ttcccaatta gatttttaaa agtttttcct
    12061 taaatgtgtc tttattgata cagagaagct tctttaataa aacaatgcct ccctaaattt
    12121 tcttgagata ggaaaggtga acatacaaaa taatacgacc actttgaaaa cccagatgat
    12181 ccacaaatgg atcgtatgat actgatgaag taaaaagcaa tggatcagaa actcaaagag
    12241 attgaaaaag acaaggaaga aataatatct cttttttact cttgtctgtt ttagccaata
    12301 atagctcgta aagtaatcta gacaaacaaa tagaagtaaa caaagtttcg taagagcagc
    12361 aatcgtatca tgttgctctg ctagacgatt gacgagcagg aaaaatgttg gtaatggaat
    12421 atagatcacg agtaatgaaa gaaaaaagta aacaataaat ggcatactat tcctcctaat
    12481 tgtgtgagtt cgccctcatt ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg
    12541 taacaaatgt tatctaatgt atttttaaat aaataatcat tattatcaaa ataatg
//
LOCUS       DAFELO010000079        12554 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFELO010000079
VERSION     DAFELO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.082"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(94..969)
                     /locus_tag="LP452_002422"
     CDS             complement(94..969)
                     /locus_tag="LP452_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL2015114.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="LP452_002423"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="LP452_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL2015115.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="LP452_002424"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="LP452_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL2015116.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="LP452_002425"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="LP452_002425"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL2015117.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="LP452_002426"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="LP452_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL2015118.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="LP452_002427"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="LP452_002427"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL2015119.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="LP452_002428"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="LP452_002428"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL2015120.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="LP452_002429"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="LP452_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL2015121.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="LP452_002430"
     CDS             complement(9420..10187)
                     /locus_tag="LP452_002430"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL2015122.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="LP452_002431"
     CDS             complement(10180..11040)
                     /gene="ylqF"
                     /locus_tag="LP452_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL2015123.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11300..11698
                     /locus_tag="LP452_002432"
     CDS             11300..11698
                     /locus_tag="LP452_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2015124.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11977..12477
                     /locus_tag="LP452_002433"
     CDS             11977..12477
                     /locus_tag="LP452_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL2015125.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT         3318 a         2692 c         2203 g         4341 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    10681 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    10741 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    10801 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    10861 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    10921 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    10981 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    11041 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    11101 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    11161 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    11221 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    11281 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    11341 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    11401 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    11461 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    11521 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    11581 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    11641 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    11701 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    11761 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    11821 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    11881 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    11941 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    12001 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    12061 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    12121 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    12181 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    12241 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    12301 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    12361 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
    12421 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    12481 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    12541 ctgcacgctt tatt
//
LOCUS       DAFELO010000080        11879 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFELO010000080
VERSION     DAFELO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.083"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LP452_002434"
     CDS             complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LP452_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL2015126.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1819..3042)
                     /locus_tag="LP452_002435"
     CDS             complement(1819..3042)
                     /locus_tag="LP452_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL2015127.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3073..3912)
                     /locus_tag="LP452_002436"
     CDS             complement(3073..3912)
                     /locus_tag="LP452_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL2015128.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3929..4609)
                     /locus_tag="LP452_002437"
     CDS             complement(3929..4609)
                     /locus_tag="LP452_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015129.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4611..5684)
                     /locus_tag="LP452_002438"
     CDS             complement(4611..5684)
                     /locus_tag="LP452_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2015130.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5740..5978)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6021..6254)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6331..7002)
                     /locus_tag="LP452_002439"
     CDS             complement(6331..7002)
                     /locus_tag="LP452_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2015131.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="LP452_002440"
     CDS             complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="LP452_002440"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL2015132.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10426..10863
                     /gene="ndk"
                     /locus_tag="LP452_002441"
     CDS             10426..10863
                     /gene="ndk"
                     /locus_tag="LP452_002441"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL2015133.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10885..11751)
                     /locus_tag="LP452_002442"
     CDS             complement(10885..11751)
                     /locus_tag="LP452_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2015134.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         3435 a         2584 c         2007 g         3853 t
ORIGIN      
        1 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
       61 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
      121 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
      181 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
      241 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
      301 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
      361 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
      421 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
      481 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
      541 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
      601 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
      661 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
      721 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
      781 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
      841 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
      901 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
      961 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     1021 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     1081 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     1141 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     1201 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     1261 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     1321 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     1381 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     1441 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     1501 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     1561 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     1621 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     1681 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     1741 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     1801 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     1861 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     1921 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     1981 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     2041 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     2101 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     2161 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     2221 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     2281 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     2341 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     2401 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     2461 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
     2521 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
     2581 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
     2641 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
     2701 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
     2761 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
     2821 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
     2881 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
     2941 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
     3001 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
     3061 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
     3121 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
     3181 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
     3241 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
     3301 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
     3361 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
     3421 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
     3481 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
     3541 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
     3601 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
     3661 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
     3721 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
     3781 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
     3841 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
     3901 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
     3961 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
     4021 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
     4081 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
     4141 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
     4201 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
     4261 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
     4321 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
     4381 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
     4441 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
     4501 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
     4561 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
     4621 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
     4681 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
     4741 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
     4801 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
     4861 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
     4921 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
     4981 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
     5041 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
     5101 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
     5161 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
     5221 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
     5281 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
     5341 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
     5401 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
     5461 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
     5521 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
     5581 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
     5641 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
     5701 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
     5761 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
     5821 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
     5881 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
     5941 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
     6001 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
     6061 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
     6121 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
     6181 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
     6241 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
     6301 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
     6361 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
     6421 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
     6481 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
     6541 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
     6601 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
     6661 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
     6721 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
     6781 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
     6841 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
     6901 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
     6961 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
     7021 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
     7081 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
     7141 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
     7201 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
     7261 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
     7321 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
     7381 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
     7441 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
     7501 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
     7561 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
     7621 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
     7681 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
     7741 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
     7801 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
     7861 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
     7921 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
     7981 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
     8041 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
     8101 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
     8161 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
     8221 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
     8281 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
     8341 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
     8401 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
     8461 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
     8521 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
     8581 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
     8641 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
     8701 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
     8761 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
     8821 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
     8881 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
     8941 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
     9001 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
     9061 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
     9121 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
     9181 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
     9241 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
     9301 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
     9361 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
     9421 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
     9481 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
     9541 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
     9601 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
     9661 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
     9721 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
     9781 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
     9841 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
     9901 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
     9961 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    10021 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    10081 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    10141 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    10201 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    10261 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    10321 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    10381 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    10441 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    10501 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    10561 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    10621 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    10681 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    10741 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    10801 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    10861 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    10921 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    10981 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    11041 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    11101 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    11161 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    11221 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    11281 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    11341 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    11401 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    11461 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    11521 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    11581 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    11641 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    11701 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    11761 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    11821 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatg
//
LOCUS       DAFELO010000081        11152 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFELO010000081
VERSION     DAFELO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.084"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            258..533
                     /locus_tag="LP452_002443"
     CDS             258..533
                     /locus_tag="LP452_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015135.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            546..2624
                     /locus_tag="LP452_002444"
     CDS             546..2624
                     /locus_tag="LP452_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL2015136.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMLQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2784..4670
                     /locus_tag="LP452_002445"
     CDS             2784..4670
                     /locus_tag="LP452_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2015137.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4682..5629
                     /locus_tag="LP452_002446"
     CDS             4682..5629
                     /locus_tag="LP452_002446"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL2015138.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5649..6176
                     /locus_tag="LP452_002447"
     CDS             5649..6176
                     /locus_tag="LP452_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2015139.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6239..6892)
                     /locus_tag="LP452_002448"
     CDS             complement(6239..6892)
                     /locus_tag="LP452_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL2015140.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7024..7866
                     /locus_tag="LP452_002449"
     CDS             7024..7866
                     /locus_tag="LP452_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2015141.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8024..8920
                     /locus_tag="LP452_002450"
     CDS             8024..8920
                     /locus_tag="LP452_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2015142.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8923..9636
                     /locus_tag="LP452_002451"
     CDS             8923..9636
                     /locus_tag="LP452_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL2015143.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9656..10174
                     /gene="msrA"
                     /locus_tag="LP452_002452"
     CDS             9656..10174
                     /gene="msrA"
                     /locus_tag="LP452_002452"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2015144.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10171..10398
                     /locus_tag="LP452_002453"
     CDS             10171..10398
                     /locus_tag="LP452_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL2015145.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10550..11101
                     /gene="lepB"
                     /locus_tag="LP452_002454"
     CDS             10550..11101
                     /gene="lepB"
                     /locus_tag="LP452_002454"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2015146.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         3868 a         1881 c         2301 g         3102 t
ORIGIN      
        1 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
       61 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
      121 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
      181 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
      241 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
      301 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
      361 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
      421 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
      481 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
      541 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
      601 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
      661 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
      721 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
      781 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
      841 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
      901 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
      961 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     1021 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     1081 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     1141 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     1201 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     1261 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     1321 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     1381 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     1441 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     1501 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     1561 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     1621 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     1681 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     1741 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     1801 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     1861 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     1921 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     1981 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     2041 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     2101 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     2161 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     2221 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     2281 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     2341 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgctcc
     2401 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     2461 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     2521 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     2581 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     2641 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     2701 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     2761 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     2821 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     2881 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     2941 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     3001 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     3061 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     3121 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     3181 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     3241 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     3301 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     3361 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     3421 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     3481 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     3541 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     3601 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     3661 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     3721 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     3781 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     3841 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     3901 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     3961 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     4021 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     4081 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     4141 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     4201 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     4261 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     4321 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     4381 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     4441 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     4501 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     4561 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     4621 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     4681 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     4741 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     4801 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     4861 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     4921 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     4981 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     5041 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     5101 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     5161 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     5221 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     5281 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     5341 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     5401 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     5461 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     5521 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     5581 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     5641 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     5701 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     5761 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     5821 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     5881 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     5941 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     6001 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     6061 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     6121 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     6181 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     6241 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     6301 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     6361 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     6421 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     6481 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     6541 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
     6601 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
     6661 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
     6721 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
     6781 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
     6841 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
     6901 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
     6961 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
     7021 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
     7081 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
     7141 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
     7201 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
     7261 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
     7321 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
     7381 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
     7441 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
     7501 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
     7561 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
     7621 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
     7681 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
     7741 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
     7801 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
     7861 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
     7921 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
     7981 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
     8041 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
     8101 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
     8161 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
     8221 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
     8281 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
     8341 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
     8401 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
     8461 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
     8521 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
     8581 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
     8641 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
     8701 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
     8761 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
     8821 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
     8881 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
     8941 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
     9001 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
     9061 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
     9121 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
     9181 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
     9241 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
     9301 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
     9361 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
     9421 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
     9481 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
     9541 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
     9601 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
     9661 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
     9721 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
     9781 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
     9841 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
     9901 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
     9961 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    10021 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    10081 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    10141 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    10201 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    10261 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    10321 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    10381 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    10441 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    10501 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    10561 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    10621 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    10681 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    10741 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    10801 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    10861 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    10921 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    10981 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    11041 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    11101 agaaacaatg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa ta
//
LOCUS       DAFELO010000082        10155 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFELO010000082
VERSION     DAFELO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.085"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(560..1600)
                     /locus_tag="LP452_002455"
     CDS             complement(560..1600)
                     /locus_tag="LP452_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2015147.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="LP452_002456"
     CDS             complement(1949..2275)
                     /locus_tag="LP452_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015148.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="LP452_002457"
     CDS             complement(2262..3065)
                     /locus_tag="LP452_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2015149.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="LP452_002458"
     CDS             complement(3418..3708)
                     /locus_tag="LP452_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL2015150.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="LP452_002459"
     CDS             complement(3844..4023)
                     /locus_tag="LP452_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015151.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="LP452_002460"
     CDS             complement(4020..4370)
                     /locus_tag="LP452_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015152.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="LP452_002461"
     CDS             complement(4363..5688)
                     /locus_tag="LP452_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2015153.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="LP452_002462"
     CDS             6138..6752
                     /locus_tag="LP452_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015154.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7921)
                     /locus_tag="LP452_002463"
     CDS             complement(6917..7921)
                     /locus_tag="LP452_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015155.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8380..8637)
                     /locus_tag="LP452_002464"
     CDS             complement(8380..8637)
                     /locus_tag="LP452_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2015156.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8630..8899)
                     /locus_tag="LP452_002465"
     CDS             complement(8630..8899)
                     /locus_tag="LP452_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2015157.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9030..9209)
                     /locus_tag="LP452_002466"
     CDS             complement(9030..9209)
                     /locus_tag="LP452_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015158.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9590..>10155)
                     /locus_tag="LP452_002467"
     CDS             complement(9590..>10155)
                     /locus_tag="LP452_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015159.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3101 a         1854 c         1458 g         3742 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca
     7201 tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt
     7261 tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat
     7321 taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct
     7381 caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac
     7441 aggctgtaat tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa
     7501 ggagtataag agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag
     7561 cttcagaagt tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta
     7621 atgaaatttc tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt
     7681 cgtcagcaag catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca
     7741 tttcagcaat attgctaaaa tcagctaatt tttctttaga ataaaaaaaa acatttatat
     7801 cagaatcaat aaattctatt ttttttatta ctatatcgtc atcattatct tgaccattta
     7861 tgtttattgt tccctgagtg gggagtactt ctttcaaatt caagttaaat ttatattcca
     7921 ttacttctcc tccaatatat aaaaattact taactgtatc caaatttaat atgtaaataa
     7981 atcattaatt tttagctaaa ttctatgaga agtttatttt tgttgttttt gctaattaac
     8041 taaaactagt acaaaaaatt taaaatagat tattaaacct attgcttatt ctattcagtc
     8101 atatatataa gcgtcatcct ttagtaacta acatcccaca ctacaatagt attataacat
     8161 atctattcaa cttcaaattg ttgaaattta agccatttat atttactttt cctcaacttt
     8221 agaaatgatt ttactaacct tatatccgta atacgcgtaa caaacacatt cattaataac
     8281 aatcaacact aagctatatt agctttttaa atgactaggg taaataaaat aaggatcaaa
     8341 aacttttaag tttctgaccc tttccttact tccgattggt taatagtgat cttttgcaga
     8401 ataaataaat atcgtttcat tttcaactct atatatcagt ctatgttcat ctgtaattct
     8461 tctggaccat tttccagata aatcatgctt taatggctca ggttttccta atccagcaaa
     8521 gggggaacga tcgatatctt ttattaactt gttaatcttt tttatattgc ttttgtttcc
     8581 ttgctcatgc caataaagat aatcatccca agcatcatca gaccaagcct taatcatcag
     8641 attcaacctc gattaagtca tgtgttttaa atgcaccttt ggagaattgt tcatctcctc
     8701 gacgaatttt ttccatgacg taattattag aaagtgttct caacgtttct tgcatagaat
     8761 cataatctct ttttgataat acaacaactg tatcttctac atctttactt gttacaataa
     8821 gtgtttcagc atcctcatta acttgtttca tataactacg taaattttgg cggaaatttg
     8881 aataagctac tgcttccatt cctttcaccc cttatttatt tctataaaac aattgtacat
     8941 tatattgtac aattaagcaa atgattaata ttctaacttc ttataattaa aagtgctatc
     9001 acttttagtt tttaggataa aaagtgacgt cactttttga gtgctggtac ctcaaatcct
     9061 gctttttctt gggcaatcaa taaatctaac gcaatacaca taatcctgga ttcacttttg
     9121 ccgtacttct gctttagttg ctgcaattct ttttgtctct tttcatccaa cgtaacgggt
     9181 aaccgtttat ttcctattgc aattggcatt agtactctct cctttcatcg aaataaccct
     9241 atgaaaaata tggctgcaat catcttgaat agtagactca caagggctaa tacaaagcgc
     9301 caaaagatca aacaaaatac cagtattgcc aaaaatacta cgaagtttaa atgcacagct
     9361 gcagcatggc gcataagtat ttcgccaaca aataggacta acaatagagg aatgatttta
     9421 ttcatatgga tctccttctt tctcttaggt tatcgggtgt tgcccgctta tttcgcacca
     9481 cctaagcggc gggggcggtt ggagcgattg gtgcgaaata agaaccattc ccttagttac
     9541 taggtttttt gtccttattt cacacattct cacttttcgg tataaatgat tattgccttt
     9601 ttttcctttc aaatcgttga tatggcattc gtttacggtc aagttcattt tggatttctt
     9661 ctgtatcgtc gtaagtatag gtgtctaaaa aatcagttaa agtcgtgtac cagtcgtcgc
     9721 ctgggttatc tacttcttct acatattccc tcaaaaatgc ttcgggtaca gcgagttcat
     9781 aatccattcc cattaacacg atagattccg ttccacacgt caaaaatcta gtaaatacaa
     9841 ggtaataatc gtctatcgtc gcatctttgc tgtttagctc ttccatttct tcttccatta
     9901 attccatgct ctcaaaaatt gaataatatt ctgattctga gtaatagtgt ttcctgcaaa
     9961 tcgtgtagct gacaaagttt tcgaccagct cactcctttc taggtaaaca ttcatgatgt
    10021 atttttctgt ttctagtcgt agaagctccg ttcctttttt taccgtttca ggacgacgtt
    10081 tatacacgtc taaacgatta tggctgactg tgaccttcgt ttgactcgtt tcactttcca
    10141 aatacaattg ttctt
//
LOCUS       DAFELO010000083         9413 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFELO010000083
VERSION     DAFELO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.086"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(42..680)
                     /gene="plsY"
                     /locus_tag="LP452_002468"
     CDS             complement(42..680)
                     /gene="plsY"
                     /locus_tag="LP452_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL2015160.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            807..1241
                     /locus_tag="LP452_002469"
     CDS             807..1241
                     /locus_tag="LP452_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL2015161.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1407..3452
                     /gene="parE"
                     /locus_tag="LP452_002470"
     CDS             1407..3452
                     /gene="parE"
                     /locus_tag="LP452_002470"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL2015162.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3464..5914
                     /gene="parC"
                     /locus_tag="LP452_002471"
     CDS             3464..5914
                     /gene="parC"
                     /locus_tag="LP452_002471"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL2015163.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6191..8422
                     /gene="pflB"
                     /locus_tag="LP452_002472"
     CDS             6191..8422
                     /gene="pflB"
                     /locus_tag="LP452_002472"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL2015164.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8666..>9413
                     /gene="pflA"
                     /locus_tag="LP452_002473"
     CDS             8666..>9413
                     /gene="pflA"
                     /locus_tag="LP452_002473"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL2015165.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTK"
BASE COUNT         3323 a         1627 c         2037 g         2426 t
ORIGIN      
        1 tttgtcacac tcccgtttta tctctttgga atgaaaaagt atcaggattt tttcttgctg
       61 ctattcaaac caaacgggat tcgactttca gtatggtttc tgatacgctt gatattgtct
      121 ttatgccgat aaaagataaa ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg
      181 ggcttagcca aaatcacagg agctacataa ggaaggacga tcgtagataa agtgatcaat
      241 actgcactga tcatactgct caaactcacc atacttgtac aaaataaact aacgataaag
      301 atgatacaag aataaacaaa aaatacagga ttaaatccta aaagcattcc tgcacttgtc
      361 gcaacagctt ttcctccttt gaacccagca aaaatcggaa atgtatggcc aagaatggcg
      421 cataccccaa accaaagagg gttaatccca gaaaggtgga agatcatcgg caaacaagta
      481 gccaatgtac cttttaaaat atccataagc aggacaacaa tcccggctgt tttgcctaat
      541 acacgaaatg tattggtcgt tccagtatta ccgctcccgt gttgtcggat atcttttttg
      601 aaaaacaatt tgccaatcca gacaccggaa ggaatagaac ctaaaagata tgctaaaata
      661 agtaataaga tcaatttcat tttttaccct ctttcaagtt gaaaactatc ctttatttta
      721 gcatgaatag gtaacaggaa caattgaaaa aatgaaagat acaagtataa tagatggaat
      781 aaaaagaaaa gcaaggggtg gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt
      841 tcaactattt ggaagaagca aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac
      901 gaacaagtta catggttgca caggtattgc aagacaatgg atataaaatc ataccagtaa
      961 atccattatt ggctggtgaa gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa
     1021 tcgatgatcc aatcgatatc gtagatgtat ttagacggag tgaattttta cctgaaatag
     1081 caggtgattt tttgaaaact gatgctaaag tgttctgggc acaattaggg attgaaaaca
     1141 aagaagcagc tgagatgcta agaaaagcag gacgtacaga tatcgtaatg gatcgttgta
     1201 ttaagattga acttggtaag ttgaaagaaa accaaaaata aaaatgacca caaagaagag
     1261 atgatcgata gaggggaatg tcgaaaaagc ggttaggaga taattaaaat tgatttctcg
     1321 tctaccctct tttttttcac gaccttttct agtatgataa tcgtggtgcg gttttgccgc
     1381 aaagtattat tgaaggagcg tttgccttgg caaaaaaagt aaacaacgaa tacaatgact
     1441 cgtccatcca agtattggaa ggattagagg cagttagaaa aagaccaggg atgtacattg
     1501 gatccactga cagccgaggc ttgcatcatt tagtctatga aatcgtcgat aatgcagttg
     1561 acgaagcatt atcaggttat ggcagtgaga ttgatgtgac catccatgaa gacaatagta
     1621 taactgttgc agatagcgga cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa
     1681 cagttgaagt gatttttact gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca
     1741 aaacttctgg tggactgcac ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc
     1801 taattgtaac gatcgttaga gatggcgtag agtatcagca gaaatttaaa aacggcggta
     1861 aacctgacgg tactttgaaa aagataggaa aaacaaaaaa agcaaacggg acgactgtcc
     1921 attttttacc agatgataca atattcagta caacgaaatt ttcatatgaa attttagccg
     1981 aacggttaag agaatctgcc tttcttctaa aaggcgttaa aatctctctt tctgatcttc
     2041 gtggagaaga accagtcaag gaaatttttc attacgaaga aggaatcaaa gaatttgttg
     2101 attatctgaa tgaagaaaaa gatacattga caccagttgt ttatttttca ggagaaaaag
     2161 aaggtatcga agtagaggtc gcttatcaat acaatgacgg ttattcagaa aatgttttat
     2221 cttttgtcaa taacgttcgg acaaaagatg ggggaaccca tgaagctggt atgaaagcgg
     2281 cgatgacgaa atcttataat gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca
     2341 agaatctgga aggtagtgat ttcagagaag ggttagctgc tgtactttct atccgtgtac
     2401 ctgaaaatct tctgcaattt gaagggcaaa caaaagaaaa acttggtacg ccagttgctc
     2461 gtaccgtagt agataacgtc atcagtgaac aaatgggctt ttatttgcag gaaaatagcg
     2521 aaatgagcca gatgttggtc cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac
     2581 gtaaagccag agaagaaagc cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt
     2641 caggaaaatt gacacccgca caatcccgaa accctaagaa aaatgaattg tatttagtcg
     2701 aaggggattc tgctggcgga tctgctaaac aaggaagaga cagaaagttc caagcaattc
     2761 tacctttacg aggaaaagtc atcaataccg aaaaagctaa aatgcaagat atcttgaaaa
     2821 atgaagaaat caatacgatg atctatacga ttggtgcagg agtcggtcct gagttttcta
     2881 tcgaagactg taattacgac aaagtaatca tcatgaccga tgcggataca gacggcgccc
     2941 atattcaagt attactgctt actttctttt atcgttatat gaaaccgctg attgaagctg
     3001 ggaaagtata tatcgcgtta ccgcctttat ataaagtatc caaaggacaa ggaaagaagc
     3061 aagtcattga atatgcgtgg acagatgacg aattggcagc aatgattaaa aaagtcggca
     3121 aaggatatat gctgcagcga tataaaggac ttggagagat gaatgcggaa caattgtggg
     3181 aaacaacgat ggatccgact tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc
     3241 aagctgaacg tcgagtaacc acactgatgg gtgacaaagt agaacctcga agaaaatgga
     3301 tcgaaaatca cgtgcaattt acgctcgaag aagatggcag tatcttagat aaaaaagagg
     3361 atactgaaat ctctccttca gtttctaatg atttattaga tgaagaacga gcagataaaa
     3421 atgaaaacaa tcagttattt gaagtagaat aggaggtcac ctgatggaaa atcgacaaga
     3481 agtacaagaa ttaactttag aagaagtgat gggtgaccgc ttcggacgtt attccaaata
     3541 tattatccaa gaacgtgcac tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg
     3601 gatattattt gccatgaaca aagatggcaa tacgtatgac aaaggattcc gtaaatcagc
     3661 aaaatctgtc ggaaatatca tggggaatta tcatcctcat ggtgaccgca gtatttatga
     3721 agcgatggtc cgcatgagcc aagactggaa gttgcgggaa gtactgatcg aaatgcacgg
     3781 gaataacggg agtatggacg gtgatccacc tgctgcgatg cgttatactg aagccagatt
     3841 atccaagtta agtggagaga tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg
     3901 gaattttgat gatacagaaa aagaaccaac agtccttcca gctcgatacc caaaccttct
     3961 cgtcaatgga tcgacaggta tatctgctgg atatgcaaca gaaatcccga ctcataactt
     4021 agctgaagtg atcgatggca caatctatat gatcgatcat ccacaagcga gccttgaaaa
     4081 attgatggaa tatattcctg gacctgactt tccaacagga gggatcttgc aaggaaaaga
     4141 tgaaatcaaa aaagcttatg aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa
     4201 gatagaggca atgaagggta ataaacagca aatcgtgatc acagaaatcc catatgaagt
     4261 aaataaagcg accttggtga aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg
     4321 aatagctgaa gtacgagatg aaagtgatag aacaggtctg caaattgtcg tcgaattgaa
     4381 aaaagacgcc aatgctcaag gaattttgaa ttatttgttc aaaaacactg agctacagat
     4441 caattacaat ttcaatatgg tcgctatcga ccatatgact ccgcatcaag ttggactgaa
     4501 agacatttta agaagttata tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt
     4561 cgatttagcc aaagctcaaa aaagacagca tatcgtagaa ggattgatga aagctttatc
     4621 gattctggat gaagtaatcg caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa
     4681 gaatttagtc gacgtctttc aatttacaga agaacaagca gaagcaattg tcacattgca
     4741 attataccgt ttgacgaaca cggatatcac agagcttcaa aaggaatccg aaagcttgat
     4801 tgcgcaaatc acagagttga ataaaatcct ttcaaatgac aaagagcttt tctctgttat
     4861 gaaaaaagaa ttgcgagaag tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga
     4921 agacgaaatc gaagaaatca aaattgatac gcaagtctta gtggcacaag aagacgtaat
     4981 tgtcagtgtg acgcgagaag gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc
     5041 caaaccagaa gaaatcggga tgcgagaagg cgactacttg ctatactcag gtgaattgag
     5101 tacattggac catgttcttc tgatcacaaa taaagcaaat gtgatctacc gccctgtcca
     5161 tgaactaccc gatttgaaat ggaaagatgc tggtgagcac atttctcaaa caatctccaa
     5221 tctgtctgta gacgaatcga ttttagctgt tttcccatat caaaaaattc aagcagagaa
     5281 aacatttgta ttcatcagca agaatggatt gatcaagcaa acacgaatga cagagttcga
     5341 accatggcgc acctataaga gccgtccgct cagctgtatg aagctaaagg cggaagaaga
     5401 tgaactgatt gctgtgtatc tgaaagaaga tcagccaggc ttagatgttt tcttagtaac
     5461 gcatcaagga atgggtttgc gctatccttt aacagatgtt cccgttgttg gagcaaaggc
     5521 agctggagtg aagtctatga atttgaaaga tggagatagg attatcaacg ggctgcttgt
     5581 gttagcagaa ggtgatacgc cgatcgtaat cgttactcag cgaggagctg ttaaacgcat
     5641 gcttgctcaa gaaatcagcc aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga
     5701 gctgaaaaaa aatccgcacc gtattatcta tatgtcggaa ggaagatcaa aagaaataac
     5761 cttgatgaat caaaaagggc agcagttaac gattgaccca actgatttcc ctataggtga
     5821 tcgaacatcg aatggatcat ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt
     5881 tatcgatgct cctgagattt tcatcgagtc gtaactgttg tagtatttta atgaagagac
     5941 gccatctgta ttaatacagg tggcgtctct ttttcattca tttttaataa atccttatac
     6001 gtttgcaaaa aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt
     6061 taaatatatg taaataaaac ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa
     6121 gatagggtgc tgtgttatac taatcacgta aaaactgtta cataaaacag aaatttagga
     6181 ggtccataca atggagcaat ggaatggatt taaaggtacc aaatggaaag aaggcgtaga
     6241 cactagagac tttattcaaa ataactacac tgaatatcgc ggtgatgaca gctttttgga
     6301 aggcccaaca gaagcaacag aaaaattatg ggatgaattg caaaagttaa atgatatcca
     6361 attcgagcgt aacggtgtct atgatatgga cacagaaatc gtatcaacta ttacttcaca
     6421 cgatgcaggc tatttggata aagatttgga aaaaatcgtc ggtctacaga cagacaaacc
     6481 tttgaaacaa gcattcatgc cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag
     6541 caatggctat acaccaaacg atgaattgac acatattttc actgactggc gcaaaacaca
     6601 taatcaaggt gtatttgacg cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat
     6661 cattacaggg cttccagacg cttacggtcg cggacgtatc atcggtgact atcgtcgtgt
     6721 ggcattgtat ggaatcgact atttgatgga acaaaagaaa aaagatcatg atatgactgg
     6781 ttatcataca atgagtgaag aagtgattcg tttacgtgaa gaaatcactg aacaatatcg
     6841 tgcattagcg caaatgaaag aaatggctgc gaaatatggc tatgatattt ctaaaccagc
     6901 tgcaaatgca aaagaagctg tccaatggct atacttcgga tatttagcag caatcaaatc
     6961 acaaaatggt gctgcgatgt caatcggacg tatctcagca ttccttgata tctatatcca
     7021 acgtgatttg gataatggag taatcacaga aaaagaagca caagaattga tcgaccactt
     7081 agtaatgaaa ttacgtatgg tcaaattcgc gcgtacacct gaatataacc aattattctc
     7141 aggattccct atctgggcta ctttatccat tgcaggtatg ggattagatg gtcgttcatt
     7201 agttacaaag aatgatttcc gtctgcttca tacattaaca aatatgggac cttcaccaga
     7261 accaaacttg actgttttgt attctgaaca ccttccagaa ggattcagaa catatgctag
     7321 cagaatcgct atcgaaagtt cttcgattca attcgaaaat gataatcagc ttcgtgaaca
     7381 atggggttct gatgattgtg cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga
     7441 catgcaattc tttggcgctc gtgctaactt ggctaaaaca ctattatatg ccatcaatgg
     7501 cggagtcgat gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga
     7561 aatactagac tttgaagaag tgaaacataa cttcatgaat atgatggatt ggttagcaga
     7621 actatatgtc aatacgttga acatcattca ttatatgcat gataaatatt cttatgaagc
     7681 agctcagctg gctttgatgg attctgactt gcaacgcaca tttgctacag gtattgctgg
     7741 gatctctcat gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg
     7801 taatgaaaaa ggaatagcag ttgattttga agtggatggc gacttcccta aatacggaaa
     7861 tgatgatgac cgtgcagatg aattagtttc atggatcttg gaatacttca tgggacaaat
     7921 caaacgtcat aaaacttacc gcaactcaaa accaacaact tcattattga cgattacttc
     7981 aaatgttgtt tacggtaaaa atacaggaaa tactcctgat ggccgtgaag ctggaaaacc
     8041 acttgcgcca ggagcaaatc caagttacaa tgcggaacaa agtgggttat tagctagcct
     8101 gaactcaaca gctaaaatga attacggctg tgcattggat ggaatttcaa atacccaaac
     8161 aatcaatcct tcaggtcttg gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt
     8221 cttagatgga tacttctcaa aaggagctta tcatttgaat gtcaatgtat tcagtaatga
     8281 tttattgcgt gatgcaatgg aaaatccaat gaaatatccg aacttgacga tccgggtatc
     8341 tggttatgca gttaaattcc gtgacttgac aagagaacaa caattagatg tcttgatgag
     8401 aacttcacac gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa
     8461 ggacgttgaa tagccatcgc atcaaaatcg aataatcagt atgcttattt gaaagaaacg
     8521 atttacaggg gcaggacttg tatgttccgt ccctgtaaat tttacacata acgaagcggc
     8581 aattgcgaaa aattttgttc agaaagcttc gcttcgacag ctaacttgta cttaaaatga
     8641 aatcaaagaa aagatgtgaa cttaaatgga agaaaaaaca atcggctatg ttcattctat
     8701 cgaaactttc ggttcagtag acggacctgg actgcgcttt gtcgtgttta tgcaaggatg
     8761 ccgcatgcgt tgccaatttt gtcacaatcc agatacttgg aatattggcg gagggaaaga
     8821 atacacagca gatgaacttt tagataaagc agaacgcttc agaccttact ggggagataa
     8881 aggtggtatt actgttagtg gtggagaacc tctattgcag atcgattttt tgatcgaact
     8941 atttaaaaaa gcaaaagagc ggaagatgca tactacattg gatacatgcg gaaaaccttt
     9001 tacgtatgaa gaacctttct tttcacgttt tcaagaattg atgaagtata cagatttact
     9061 gttatttgac atcaaacaca tcgataatga agagcataaa aaactaacgc attgggacaa
     9121 tgaaaatatt ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg
     9181 ccatgtacta gttcctgagc gaagtgatta tgatgaatat ttgatccgac tagacaactt
     9241 tattcaaacc ttgtcaaatg tagacagagt agagattctt ccttatcata cgatgggaaa
     9301 atacaagtgg gaaacacttg gtttgaaata tccattagaa gggatcgaac cacctacaaa
     9361 agaacgtgtc gaaaatgcta aacgattgct tcatacagaa gagtatacaa aat
//
LOCUS       DAFELO010000084         9388 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFELO010000084
VERSION     DAFELO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.087"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            2..77
                     /locus_tag="LP452_002474"
     tRNA            2..77
                     /locus_tag="LP452_002474"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="LP452_002475"
     tRNA            79..149
                     /locus_tag="LP452_002475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="LP452_002476"
     tRNA            163..236
                     /locus_tag="LP452_002476"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="LP452_002477"
     tRNA            261..348
                     /locus_tag="LP452_002477"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="LP452_002478"
     tRNA            370..441
                     /locus_tag="LP452_002478"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="LP452_002479"
     tRNA            464..553
                     /locus_tag="LP452_002479"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="LP452_002480"
     tRNA            566..639
                     /locus_tag="LP452_002480"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="LP452_002481"
     tRNA            658..730
                     /locus_tag="LP452_002481"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="LP452_002482"
     tRNA            734..806
                     /locus_tag="LP452_002482"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="LP452_002483"
     tRNA            811..881
                     /locus_tag="LP452_002483"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="LP452_002484"
     tRNA            894..967
                     /locus_tag="LP452_002484"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="LP452_002485"
     tRNA            979..1050
                     /locus_tag="LP452_002485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     regulatory      1239..1318
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1316..2509
                     /locus_tag="LP452_002486"
     CDS             1316..2509
                     /locus_tag="LP452_002486"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL2015166.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="LP452_002487"
     CDS             2639..4111
                     /locus_tag="LP452_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2015167.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="LP452_002488"
     CDS             4194..4982
                     /locus_tag="LP452_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL2015168.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="LP452_002489"
     CDS             complement(5049..5717)
                     /locus_tag="LP452_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL2015169.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="LP452_002490"
     CDS             5832..7544
                     /locus_tag="LP452_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015170.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="LP452_002491"
     CDS             7547..9319
                     /locus_tag="LP452_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015171.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2767 a         1643 c         2122 g         2856 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggcag
//
LOCUS       DAFELO010000085         9326 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFELO010000085
VERSION     DAFELO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.088"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..740
                     /locus_tag="LP452_002492"
     CDS             <1..740
                     /locus_tag="LP452_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015172.1"
                     /translation="RFKTLQQNHIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKY
                     FSFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFN
                     QINPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMI
                     FVSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIF
                     MIFLINLITSIIIYKKNSFKYFKENIL"
     gene            737..1357
                     /locus_tag="LP452_002493"
     CDS             737..1357
                     /locus_tag="LP452_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015173.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1471..1608
                     /locus_tag="LP452_002494"
     CDS             1471..1608
                     /locus_tag="LP452_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015174.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            2050..2430
                     /locus_tag="LP452_002495"
     CDS             2050..2430
                     /locus_tag="LP452_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL2015175.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            2412..3326
                     /locus_tag="LP452_002496"
     CDS             2412..3326
                     /locus_tag="LP452_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL2015176.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3566..4255
                     /locus_tag="LP452_002497"
                     /pseudo
     CDS             3566..4255
                     /locus_tag="LP452_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4375..4461)
                     /locus_tag="LP452_002498"
     CDS             complement(4375..4461)
                     /locus_tag="LP452_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL2015177.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            5084..5821
                     /locus_tag="LP452_002499"
     CDS             5084..5821
                     /locus_tag="LP452_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL2015178.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            5849..6388
                     /locus_tag="LP452_002500"
     CDS             5849..6388
                     /locus_tag="LP452_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL2015179.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            6404..6574
                     /locus_tag="LP452_002501"
     CDS             6404..6574
                     /locus_tag="LP452_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015180.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(6886..7347)
                     /locus_tag="LP452_002502"
     CDS             complement(6886..7347)
                     /locus_tag="LP452_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015181.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            7480..7854
                     /locus_tag="LP452_002503"
     CDS             7480..7854
                     /locus_tag="LP452_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBL2015182.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            7908..>9326
                     /locus_tag="LP452_002504"
     CDS             7908..>9326
                     /locus_tag="LP452_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015183.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDI"
BASE COUNT         3603 a         1416 c         1453 g         2854 t
ORIGIN      
        1 gtagatttaa aactttgcaa cagaaccaca tacagtttag acttgaggac tctcctcctc
       61 aagctcctta taaaaatcct aatttgaaaa atttaaaaat aaaactaata tttgttaata
      121 ataaccaaaa gtattttagt tttaatacct tatatggaaa ggcaaaagat aactatacaa
      181 taactgatcc catagttaga gttattaatc cagaaataac agaatcatta ttttggggaa
      241 atatattgac tagtgatggt ggattatttt ttgatcagaa acatacttca aaccaaaact
      301 tttttaatca aattaatccg tatataaaag aatttaacct agaaaatatc ataaattttt
      361 cttattctgt ttttcaagaa actggtgaat tattatctca aaaaaaagta aataaattaa
      421 atgcatatct acaatatctt ttaatattag gattatttat aataattaat tttcaaatga
      481 tttttgttag ttttactata aattcaaaaa aaatagcttt aaaaaaaata tttggatatt
      541 caccaataag tcaagtttta gatttatgtt tactcccttt tacaatagag ttatttgcta
      601 ctatgtgttt tcaattgata ttttcaaaaa acacttactt gtattttata atttttatga
      661 tctttttaat aaatctaatt acttctatta ttatttataa aaaaaatagt tttaaatatt
      721 ttaaggagaa tattttatga tattatcagt tgagaatttc accctgtcaa ttaataaaaa
      781 aaagatcaaa caaaattgga acttttcaat atcagaaaaa gattttatta tagtaacagg
      841 aaaaagtggt agtggaaaaa ctactctgtt aaataattta agcctaatag ataaacaata
      901 taccggtaac ttattctatt ttgaaaagaa agtttctaaa catgatatac aacgtctacg
      961 aagaaatcat attagctatt tatttcaaaa ctatgctctt cttgaaaatc aaacagtaca
     1021 atacaatttc gaattagcaa aaaagtttaa cagtaatatt tccaatgatc atatttataa
     1081 tttattaaca gatttttctt tgccaaaaga tatcttaaaa gaaaaaatct ttctcttaag
     1141 cggtggagaa caacaaagaa tagcattaat tagaaatatg ttaaaaccat ttgatatatt
     1201 atttgcagat gaacctacag gaaatttaga taaagaaaac tcaactttta taatgaactt
     1261 tctgaaaaat ttaacagaaa aagaaaaaaa agccgttgta ctagtaacac atgacttaaa
     1321 tgttctaaac tatggaacta atattataga attataattt ttacccgtga tgctagctga
     1381 tttaaaacta gcaccacggg tatttatttt atattccatc tctcctatcc caaaatttaa
     1441 ataatcactt aataaaaagg aggcacccaa gtgattttat tgaaaaaaga gtatgaaaaa
     1501 ttttatgata gacttattta ttctttgcaa ttgccaaaaa aattaaaccg tcaattacaa
     1561 aagacttatg aaactaaatc aactgaagaa tgggagagtt attgctagat ttttggtaga
     1621 agtaacaaaa agtggacata ttatcataat gactcgccga aagtctgata aaaaattggc
     1681 taaaaatatt gctgaatgtt taataaaact atcagtttta gctaaatagt tagtagttta
     1741 cacaaaaaag catttatgtt ttttgttgca aagcaacaat ttcatcttcg gtgaaataag
     1801 cgaaacttta caaaaagtgg ctagcatttg ctagcttttt ttgtattgtg gagcgtggct
     1861 tttgaggggg attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcactaaat
     1921 acgtaggtat ttggctagca aatatagagc ttgccaaacc cttttagtga gctttactca
     1981 ttgtaccttt ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg tactgaaagg
     2041 gtgggactta tgagcgaaca aaatcaaaat ttggctagca attcttctaa aaaatatacc
     2101 tatcgatccg agccaaaaca aatcagtttt cgagtgagcg aatccgaatt tgcaaagctg
     2161 aagcaatcgg ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc caaggcacaa
     2221 aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg cccaggcaat cgcgcgcgat
     2281 ttagccaaag ctggagggaa tatcaatcaa attgctaagt ggtgcaatac acaccaacat
     2341 gacgttgctt ctggtgacgc tcaacgttta tccgaaaacc taaaaatcat gcaaaaggag
     2401 ctacaaaaga tatggcaaca attaaggtaa gccgaaccac ttcttgtagt cgtgcaatta
     2461 attacgcaga accacgagca accattaaaa caggaataaa ctgtgacgtc gattatgcca
     2521 aaagtgaaat gaaacaaatt cgcatgctgt atggaaaaga cgaccgtgta caggcacact
     2581 tattgattca atcttttcga ccaggagaaa ttaccgctga aaaagctaat caactagggg
     2641 cagagtatgc aaaaaaaata gcccccgatc accaaatagc tatttacaca catacagaca
     2701 aagaccatat tcataatcac attgtcatta actccgttaa tctggagact ggaaaaaaat
     2761 ttcaagctca tggtcaagca tttctaaaca aatgttatga cctaaatgac gagatttgtc
     2821 ttgcacataa cttaccaatc actgagcgag gaaagaaacc agaaaaacga accatgtctg
     2881 aaataaaatt aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg attgcgatcg
     2941 atcaaacaat gaaaaacaaa aaaaccagaa cctatgacca attttgtaat tccctcaaaa
     3001 cattggggat tcattgcttc aatcgcggga aaaatttcac ttacgaattg atccacaaaa
     3061 agaaaaaagt acgatccaac agactaggaa aggactacga aaaggagacg attttacatg
     3121 agctggacag acgagaacga acctataacc aacagcgaac caacgagcta cgaagagctc
     3181 gagctagcat tcgcgaaact gcagaagaag tacgaagaga acaacgaact cttacgcctg
     3241 aacaaccgga aattagaccg gctcgaagaa accatgaaca acacgttgaa agaggaacag
     3301 accaccaaca agaattatcg ctctaaaacg ttaaaagaca tagaaaacga aataaagaaa
     3361 caacgtgctt ttctcgttcc ttatacgcaa gaaaggatac aaactacgat tgatttacaa
     3421 gttaaaaaac tccatcaaga gctattagac cagcgaaata aggaacagaa aaaggtgctt
     3481 attcaacgat cattggattt tcttgccccc ttatgttgct tagtcctgac actgttcgca
     3541 ttttggttag tatggggagg ctaaaatggc attttattta ccctatctac tcatttttgt
     3601 tagtatttca ggaagtattt ggctcatcta taaaattttt taaacaagat attcattaaa
     3661 aggctctaaa attcgattta agcgattttt ctttcttggc tgtatatttt ccttgataat
     3721 cgtttcaagc ggcttattcg gcgttttaga gggaaataag cgcgtttcta gaagtatttt
     3781 attaggaaat gtcactcaaa agtatgaatc agcgcgtgat aaaaagaaaa aagagcaagc
     3841 attagcacaa aaaatggaag agtttaccac ttgttatgag gaaatgaacg atatttttgt
     3901 gaatcaagaa aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa
     3961 cttgccagaa aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt
     4021 gcaatatgta aaagatacaa agatagaaga agcctgtagt gatctatttg gagacacaaa
     4081 cccttggttt gccagtgaag aagaaaaaaa agaaaaacaa caatctgtca catatgaacg
     4141 ctatgaaatc ttacttcaac aagcaactaa catgcaaaat ccgacaaaaa aagaaaccgc
     4201 cttaaactat ctaaaaagtg tcaaggaatg gttagatcag caacaacaaa attgaatttg
     4261 atttaaaatc atgttaaact gaatgtgcaa atgatcaaac attagataca cgaaagcccc
     4321 agagaggtcg gattctctgg ggcttttttc aggttgatgt cgcctttatt gattttactt
     4381 cttgtttcgg ttattcaacc aataactgaa taaagagaca caaactccta caagaagggg
     4441 gcaaatgaac agttcaaaca ttagatacac ctccttccag tcgcaagact gtcggatagg
     4501 cgacaagtta ttatatctta aaaaattaaa tttgtgtggt gtttttaagt atcacttgtt
     4561 ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc ttctcgacta gattaaggga
     4621 aattaatcaa gaattgtctt agaacggctt agacaagctt tcagagtctc acacctcctc
     4681 tttgaacaac ggttttagca aaggagccgg aggcgactgc gcagtataat atgagagcat
     4741 agcgatcaca cctttttagc gaccgtaggg agcaagtttt ccagtttaga aaaattcgcg
     4801 aacctttttg gaggaaaaag taggagtttt ttaggcggga aattcaatgt gctcttaaga
     4861 tatactgtta gatatactgt tagatatact gttaataata actgttaggg attagcaacc
     4921 ttatgggaga gtaagtattg acataggtag tgggggataa attgtcacac tatgggggat
     4981 aaattgtcac actatggggg ataaattgtc acactatggg ggataaattg tgttttacat
     5041 cctcttatgt ctatgataac ataatttatt gaggtgattt ttattgagta acgatatagt
     5101 tcgatataat aatggattaa attctgtccc tttaagaaaa tttaccccta tagatatgga
     5161 tttattttgg ggaatatgtt ctaaaatgaa aagaaaaggt acaaaagaag aaatttttac
     5221 atttaaacaa ataaaagaat taattgatta caatataaat gcacgtggcg accgttttgc
     5281 taatgattta ataaaaatgt caaaaaaaat cattactttg tcttttattt atgaagacga
     5341 aaaaacatat gaacaattaa atctgtttca aaaatttaaa atcgataaag tgaatgaaac
     5401 attattaata aaggtttctg aagaatttga atatatactt aattcaatcg gaactaattt
     5461 tactcgtttc gaattagaaa atatgacaaa cttaaagagt agctacgtta aagagttata
     5521 tcgccaactt atggctcata aagacagaag atctaaaaaa ggtcactggt ttgtaaaaat
     5581 agaagatttt agagctctat tagcaatacc agaaagttat cgcatgagag atattgataa
     5641 acaaattttt gcccaagcga aaaaagaatt tttaaacgtt aattcaactc accctgctat
     5701 ttttaaaact tttgaaatca aaaaaattaa agcctacaaa ggaaataaga taggatctct
     5761 gcaaatttat tttgaagaaa atataccttc aattacctta gataattggt tagagaaata
     5821 atacctaaca atttaggagg caattatcat gcaacccaaa gaaaccttta caataaagga
     5881 attatctgaa ttatttgaaa taagtaggca agcaatctct aaacatgttg ataaattaga
     5941 cccgtcctta ttagctaaaa atgagcgtgg ttataaggtt gtattacgtt ctggagtatt
     6001 gcaacttgct agcaacttag gtaaccaaag gttactatat aggttgcatg agtatgaaca
     6061 agaaaacgat aaaaacgttt atatatcaag caaaaacgtg attgtaaacg agttgctaga
     6121 taagttgcgt gaagaaaatg aatttttacg agaacaactt tccctcaaag agcaaaataa
     6181 ttctaatatg caaaaattac tcgaccagca acaacagctc accctacagg caaatcaaca
     6241 gatcgaaaaa ctgcaagaac agttacaatt agcttatacg gaagaaactc cagaagatga
     6301 atcaactact attctttctg aagaaacaga atcgatagaa aaacacacaa aaaagaagaa
     6361 gcaaaaatgg tggcaacttt ggaaataact ggagggaaca ttcatgacaa gaaaaaatct
     6421 tctattcggt tctattttac taggaattct aggcttcagt ccgatgttat tgtcagtaat
     6481 caataaaggg aataaacatg gtgaattgaa attgaatcca aattattaca acagtcctaa
     6541 cgaagaattg tttgtaaaag agacggataa ataataaaaa agactgctct tccgagcagc
     6601 cagttaaaaa ataatgagtg tttattcttg aaaaagcacg cgcttatgaa gcgctaccaa
     6661 aaatttacca tattttacaa aaaataacaa gtcttaaaaa aatgacagat aaaaatgcac
     6721 aaagaattag atatagataa ccgcgaaaaa tcaagcttta gaaagttgct tttagctaga
     6781 ccaattaaaa atcacaaagt gagcgtctct atccattggt agaggggcta cttggcatag
     6841 tgtacattat gtcaactatg caattaaata actaactact tcatattagc ttaataaaat
     6901 aatcaaaata actaggggga aaaacattgt ataaaaaaat ttttcatttt ttaaataaga
     6961 atagtaaata taaaaataat ttaagatatc tattactatt aatccagata gagcaaacca
     7021 agtaaatatt ccatgtgtaa agctaaaaaa attcatttga atcaattcaa ataaacctaa
     7081 tatagtaaca aatatattaa aaataatagc tgtcaatacc agtaaattat tttttttatt
     7141 ttttgaatta tcttcattta aaatagaatt tatgttagta ctataaaatt cacaaatttt
     7201 ttttagtgtc tctatatctg gtgttgtttt gttattttcc cactttgaaa tagtttgacg
     7261 tgtaacgtta aattttcttg ccacatattc ttgagtatac ccttttttca ctcttaattc
     7321 tcttagctta gatcctaaat cctccatttt ttctcctatg taaacgatta tttacataaa
     7381 attttatcat aaaaatacta acgcgccaaa aaagttcttt attttataaa acacactaaa
     7441 ataaaataat tctagtaaaa aagataggag aaataagtta tgaaaaaatt tttatgtctt
     7501 tctgctttaa tgggtgtctt attaactagt ggaggaatag ctagtgccac agaagcttta
     7561 aatctagatg tacccgaaga ccataccgcc atttacggag gaggaatgga agctacagaa
     7621 gaaggatttt cttctagtaa attgagatat gctgctggcg gcggggattt caactgtggg
     7681 gttaatggat ttaaagttta tgcaaattac taccatgcta gagctaaaca tagtgcaaca
     7741 gctaaaaatg gacgaggcgg tcaagtacga tcagttcaga aagctggagt aagagcttat
     7801 gcaacatgca atgccacact tactggaaat actggttggt ggaatgttta ttaaaaaaga
     7861 gagtttaaac tctctttttt atttacattt gaaaggaaat tcaaaatatg ttaaaaaaaa
     7921 taaacgctgt tttatcactt tttgtctgca ttacaatact atttttttct actaatgtta
     7981 taaatggtat aaagacttct gaagcgctat ataaaaatac cgctgaaatt catatgcaat
     8041 ataaagatga tatggacttt aacagttata taaacgcttt gtatgaaata tctacacaag
     8101 aaaatattaa tatttctcaa tatagtttta ctgctgttaa ccaactaact attcttgcta
     8161 caaaccctaa cgctaacaaa cactggaaat tttctaaaaa aaatatatta gcaagaaacg
     8221 attctaaagt ccattatact aataacaaaa attctaagta ccaattaaaa cttccaaata
     8281 attttttaaa tataaatatt tatccttttt ctcatgtgga aaatattgga ttaagtgaaa
     8341 tattatatgt acaaggaaac tcaaacaaat taattcctgt actgaaaaaa tatggaacac
     8401 ctactatttc caatatcaat caatcagata cttttcaaat taatactaat attgttcttg
     8461 ttatctgcta tcttagtata tttattgtaa ttactacaat tatatttgct ttttcaaaaa
     8521 tgaaagaaat cactctaaaa aaaatgttag gctataactc ttttgatact ataaaagatt
     8581 ctattaaaga gttaaacctc ttattttgtt ctttgattat aactttcatt ttatctattt
     8641 ttttgagcat atattacagt aacgaaaatt ttttcctatc atttcttcta tgtagtattt
     8701 ttcattttat aattagtttt ttatttatca tagttactct aagtatctat catttatcta
     8761 gtataaaaaa ttatctaaaa aattcacgac cacttaagtt aaatctttgt atattaaata
     8821 tctgtctatt cttaagcatg atccttctgt taatagcttc tacaaaagta ataaataccc
     8881 ataatgaagc cgaaaataat tctcttaaat attgggaaag aactactaat ttatacaaaa
     8941 caaatattac taatcaatta aatagaaaca acaccgtaga agaaaataac tacttgaaaa
     9001 aagctagtaa atttgtttat aaaatacaga agaattataa aacttttatt atcgcgccat
     9061 acaattacgc aactatccaa gagaataata aagaattttt tattggacaa aaagtttatc
     9121 caaattttga agactatgtt agtcaacctg ctggtgcagg aatatgtgtt gatttaaact
     9181 atctaaaata ttacaatcct atttcttttg aagaatctac tgacttaaat ttaataaact
     9241 ctgatccact aactacttac attctagtac ctaaaaaata taaagaatat gcaaaaatga
     9301 ttgaaaaaaa ttatttagaa gacata
//
LOCUS       DAFELO010000086         9209 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFELO010000086
VERSION     DAFELO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.089"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            470..2107
                     /locus_tag="LP452_002505"
     CDS             470..2107
                     /locus_tag="LP452_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015184.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LP452_002506"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LP452_002506"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL2015185.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="LP452_002507"
     CDS             complement(3183..3818)
                     /locus_tag="LP452_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL2015186.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LP452_002508"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LP452_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL2015187.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="LP452_002509"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="LP452_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6416..8434
                     /locus_tag="LP452_002510"
     CDS             6416..8434
                     /locus_tag="LP452_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL2015188.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9186)
                     /locus_tag="LP452_002511"
     CDS             complement(8494..9186)
                     /locus_tag="LP452_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL2015189.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         2978 a         1706 c         1445 g         3080 t
ORIGIN      
        1 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
       61 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
      121 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
      181 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
      241 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
      301 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
      361 tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg
      421 atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa
      481 caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc
      541 tctattcatg tctatgggaa tctatcatag tgtcaatcag gatacttact ttcatttatc
      601 tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata acttcgatca
      661 tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt
      721 ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt
      781 tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc
      841 gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg
      901 tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta
      961 cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt
     1021 agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt
     1081 tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac
     1141 cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc
     1201 acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct
     1261 gacacacatt ttaaataata actataacaa ctatactatt ggattattct tatttctcgg
     1321 attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt
     1381 tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca
     1441 tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttca
//
LOCUS       DAFELO010000087         8654 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFELO010000087
VERSION     DAFELO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.090"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="LP452_002512"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="LP452_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL2015190.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="LP452_002513"
     CDS             complement(738..1460)
                     /locus_tag="LP452_002513"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL2015191.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="LP452_002514"
     CDS             complement(1596..2477)
                     /locus_tag="LP452_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL2015192.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP452_002515"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP452_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL2015193.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="LP452_002516"
     CDS             3717..4196
                     /locus_tag="LP452_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL2015194.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="LP452_002517"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="LP452_002517"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL2015195.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="LP452_002518"
     CDS             5497..5625
                     /locus_tag="LP452_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL2015196.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5629..6174
                     /locus_tag="LP452_002519"
     CDS             5629..6174
                     /locus_tag="LP452_002519"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL2015197.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LP452_002520"
     CDS             complement(6628..8319)
                     /locus_tag="LP452_002520"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL2015198.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2403 a         1771 c         1568 g         2912 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaaccca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataa
//
LOCUS       DAFELO010000088         8573 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFELO010000088
VERSION     DAFELO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.091"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP452_002521"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP452_002521"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL2015199.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LP452_002522"
     CDS             complement(1098..2201)
                     /locus_tag="LP452_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL2015200.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LP452_002523"
     CDS             complement(2291..3247)
                     /locus_tag="LP452_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL2015201.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LP452_002524"
     CDS             3528..5462
                     /locus_tag="LP452_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2015202.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LP452_002525"
     CDS             5919..6335
                     /locus_tag="LP452_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL2015203.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LP452_002526"
     CDS             6349..6915
                     /locus_tag="LP452_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL2015204.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LP452_002527"
     CDS             6938..7924
                     /locus_tag="LP452_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL2015205.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8573)
                     /gene="scm"
                     /locus_tag="LP452_002528"
     CDS             complement(8079..>8573)
                     /gene="scm"
                     /locus_tag="LP452_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL2015206.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSK
                     TSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYK
                     KSKR"
BASE COUNT         2519 a         1654 c         1742 g         2658 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt ggtagaggta gtatctgtac tgtcacttga agctgacgtg
     8461 tccgtgctat cacttgttgt ggtagatgta tccgtactgt cgcttgaagc tgacgtgtcc
     8521 gtgctatcac ttgtggtaga ggtagtatct gtactgtcac ttgaagctga tgt
//
LOCUS       DAFELO010000089         8246 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFELO010000089
VERSION     DAFELO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.092"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="LP452_002529"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="LP452_002529"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2015207.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="LP452_002530"
     CDS             complement(1347..1982)
                     /locus_tag="LP452_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL2015208.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="LP452_002531"
     CDS             complement(2248..3300)
                     /locus_tag="LP452_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2015209.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="LP452_002532"
     CDS             complement(3313..4251)
                     /locus_tag="LP452_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2015210.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4702..5352)
                     /locus_tag="LP452_002533"
     CDS             complement(4702..5352)
                     /locus_tag="LP452_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015211.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5452..5961)
                     /locus_tag="LP452_002534"
     CDS             complement(5452..5961)
                     /locus_tag="LP452_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL2015212.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6046..6525)
                     /gene="rlmH"
                     /locus_tag="LP452_002535"
     CDS             complement(6046..6525)
                     /gene="rlmH"
                     /locus_tag="LP452_002535"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL2015213.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            6953..8242
                     /locus_tag="LP452_002536"
     CDS             6953..8242
                     /locus_tag="LP452_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL2015214.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         2616 a         1674 c         1318 g         2638 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatctgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catctattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt
     4681 attatcatga aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt
     4741 tttattaatt agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac
     4801 cggaattatc caagcccagt ccaaataatt ttttaaaatc tcatcatatt ttgcaaatat
     4861 ttcattgcca tattccgtcc ttaaaattat attttcagtg ccagacaagt gatttgtatt
     4921 gagttgataa aaaagatctc catctaaaaa aatatcatta gaagaagatt tccccacagt
     4981 aatgaccatt ccatcagctc tatgtgttac atacaaataa ttgtatgacc tcatttcacc
     5041 taatgaatca attatttctt gccctttagc tctaatatat tgatctctat tacctatctc
     5101 actcttattt aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta tttttgtaaa
     5161 ttcgcttcta ctactaatcc aatccgagat taatttttca acatgtccca gagtaaactc
     5221 tgatttattc gctaaatact ttttttgttg tattaaagac atctttcccc gaagctcaaa
     5281 attgagccta catcgattct ctatctcttc aaaaagcatc ttatcttcat cattcttata
     5341 ttcatcattc ataaaaccaa tccagctcct cgattaagat aatattttaa tctcatacaa
     5401 aaactagctc tcatcaaaac aaccattgtt ttgatttctc atgaaatttc tttatacttt
     5461 tctccgatta taaattccta accccttgag aaaattacgc atatatttat ctccgcaagg
     5521 cttaaaattt cgttggcctt cttttctcaa aaatgccccg atttcgcctt ttgatggata
     5581 gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg gtcatagaca aggcgatttt
     5641 taattttttt agcaacacat tgttgatata tttgatttcc ttgctttttt gcggcgtgtc
     5701 tactatcggc ttatcttttt gcggaccacg cgccaagaga atcaggccat ttaggaatgc
     5761 gtcaaaaact tggttgttga tggttttttc atacacatta ttttctgcat tatctttgtt
     5821 cttatcctgt actttggtca gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc
     5881 tagctcaaaa gctttaatca aatccgtatc tttcaaatca atcgcataac gcaatcgcaa
     5941 taacctatca ttgttattca tttgttcctc cgaagtattt agtctttact ttcttacgta
     6001 ctttttgtct tgctaagatt tttaggatca atgataaaaa ccgcatcact tatgataagg
     6061 cgcccccgca ttgatccgga aggcacggta gatttgctcg acaaggatca gacgcatcaa
     6121 ttgatgtgga tacgtcattt ttccaaaaga gatctgagca ttgcttcttt tcatgacggc
     6181 ttcgctgagt cctaaagagc cgccgataac aaaaacaagg tggctttttc cttggatacc
     6241 taattggtcg atttgtttag caaaagattc acttgtagga tttttccctt caattgctaa
     6301 ggcatagacg tattcttgtt ctttgatttt cgctaagatc cgttcccctt ctttttcttt
     6361 tacttgtctc atttgcgctt cgcttaaatt ctccggggct ttttcgtcag gaacttcgat
     6421 caattcgatt tttgcatagg cattcagccg tttgacatac tcattgatac cttggatcag
     6481 gtatttttct tttaattttc ctactgagat aattttgata ttcatgtttt ctctcctttg
     6541 gtcttcatgc tcactttgtt tattataatc aattttttcg tctatatgtt tattttggct
     6601 atttttgtta aaaagatttc cccaacagtt atgcacaaca tccacaggtt tatccacaga
     6661 aaacagtgtt attttacctt atttcatcac attcattagg caaacatacg ttttataaga
     6721 atttatccac tttatacaca ggctgtggat atgttttatc ccttattttt ccaatcctat
     6781 ccgcgtagtt ttacacagta tctgtggaaa aagaaaatca aatattttca tctgaaaaca
     6841 atgatttttc ctaaagtttt tcataaaaag ttcacagtct caaagtagac taaagtcaac
     6901 aaataagact atactaacag tagataaatg aatgtaattg gaggaggaac acatggatag
     6961 aaaaaatgtg acaccaaaga tgaaaaaaag taaaaataat agcctctggc gtaagttagg
     7021 tctggggctt gttggaggga tcgttggagg actactgaca gctggcattt tttatgcagc
     7081 gatgggcact ggcaacaata gttctactgc tacaagcggc aatcagaatt ctgccggtga
     7141 aacagttgtg gaaaatatca aagtcaatgt agattctgat atcaccaaag cggtagataa
     7201 agttcaagac gctgtcgttt ctgttatcaa cttacaaagt caaaaccaat caagcggttt
     7261 tggcggacta ttcgggcagc aagaagaaag tagcagctca gatgatagta atttggaagc
     7321 ttatagtgaa ggaagcggcg tgatctacaa aaaagacggg aatactgctt atgtagtcac
     7381 gaataatcac gtagtagatg gccagcaagg tcttgaagtc ttgatgaaag acggtacgaa
     7441 ggtaaaagct gaattagttg gaacagatgc ttattcagat ttagcggtgt tgaagatcaa
     7501 ttcagacaaa gtcgaaacag ttgcctcttt tggtgactca agtgctctga aggttggtga
     7561 gcctgcgatt gcgattggtt ccccactagg atctgaatat gcaaattctg tcacttcagg
     7621 aatcatttct tccttgaacc gacaagtgac aagtacgaat gaatcgaacg aaacagtcaa
     7681 tatcaatgcg atccaaacag atgcagcaat caaccctgga aattctggtg gtcctttagt
     7741 taatatcgaa ggacaagtta tcggtatcaa ctcaagtaag atcgcaagta cttctgaatc
     7801 tacttctaat gtaagcgtag aaggaatggg atttgctatt ccaagtaacg atgtagtgaa
     7861 tatcatcaat caactggaaa aagacggaaa agtgacacgt ccagctctag gtatcacaat
     7921 ggtcgactta tctgccgttt ctactcagca acaagaacaa atcttgaaga ttccttcatc
     7981 tgtaactaac ggtgtcatcg tgacaaatgt ccaaacagct acaccggctg aaaaagctgg
     8041 attgaaacaa tacgacgtca ttactaagat cgatgacaaa gaagtttctt ctggtgtaga
     8101 acttcaatct gctttgtata agaaaaaagt gggagattct gtcaaaatca cttactatga
     8161 aggctcgaaa gaaaaaacaa ctactgttga attgacgatc gatcaatcgg cattaaaaca
     8221 aaatcaaaat agtgaaaatt aataaa
//
LOCUS       DAFELO010000090         7867 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFELO010000090
VERSION     DAFELO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.094"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(81..713)
                     /locus_tag="LP452_002537"
     CDS             complement(81..713)
                     /locus_tag="LP452_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2015215.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(720..1787)
                     /locus_tag="LP452_002538"
     CDS             complement(720..1787)
                     /locus_tag="LP452_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2015216.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(1784..2515)
                     /locus_tag="LP452_002539"
     CDS             complement(1784..2515)
                     /locus_tag="LP452_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL2015217.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(2684..3163)
                     /gene="greA"
                     /locus_tag="LP452_002540"
     CDS             complement(2684..3163)
                     /gene="greA"
                     /locus_tag="LP452_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL2015218.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(3299..4474)
                     /gene="mltG"
                     /locus_tag="LP452_002541"
     CDS             complement(3299..4474)
                     /gene="mltG"
                     /locus_tag="LP452_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL2015219.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            4678..5847
                     /locus_tag="LP452_002542"
     CDS             4678..5847
                     /locus_tag="LP452_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL2015220.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(6114..7748)
                     /locus_tag="LP452_002543"
     CDS             complement(6114..7748)
                     /locus_tag="LP452_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015221.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2540 a         1457 c         1168 g         2702 t
ORIGIN      
        1 gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat
       61 tatcctcttt atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct
      121 tgtgtacggt cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt
      181 ttcagtgtaa taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc
      241 attaaaattt cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga
      301 ctcatttttt ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc
      361 gttgccctta tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct
      421 gcacctgctt caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg
      481 attttagctt ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct
      541 tccatgacta agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct
      601 tgtcgtccat tttcagcttc tccaattact tcaatgtctt cttgaataga taaataagaa
      661 gagacgccta agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc
      721 tatgcctcct tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta
      781 atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa
      841 cttcccgttt tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa
      901 agattattgt ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat
      961 gtatttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt
     1021 tttacatctt ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat
     1081 aactgctcga taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga
     1141 agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc
     1201 ttgcgaaaca tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc
     1261 atcattgctg caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt
     1321 ctttctaatt ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt
     1381 acaatttgta attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta
     1441 tcaatatctt tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg
     1501 gattcataat tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc
     1561 ttttgaataa gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac
     1621 aaatggaaga taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga
     1681 ctttgattgc tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca
     1741 atccccgaat aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct
     1801 cgacatctcc aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat
     1861 cactgctgta gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga
     1921 catttcctac taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc
     1981 gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat
     2041 tgcccaaatc tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat
     2101 agacgtcgtt tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat
     2161 gtgtttttaa ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt
     2221 tggttaaatc tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata
     2281 cagccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta
     2341 atatcaattg aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat
     2401 tgaaaatacc gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag
     2461 caaataacag cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc
     2521 tttctccctc ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag
     2581 gactttagat gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga
     2641 gtgtatcatc agtaagatga actctctaga tctttcagtc agactacgct tgttctactt
     2701 tgacgatttt aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac
     2761 gtttgccaat caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat
     2821 ctgcttcagc gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga
     2881 atgtaactgt tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt
     2941 gagcaaaacg aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat
     3001 ctttcgctga ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga
     3061 ttcgttcaat gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt
     3121 tttctttacc ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt
     3181 ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac
     3241 gcttttcttc aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct
     3301 aagaattatt tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat
     3361 aaacatttcc agtgctcgta tctgctacga aataataata gtcattttct gctggttcta
     3421 atactgcctt gattgcctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat
     3481 tggtatacaa attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt
     3541 gttcacctaa tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc
     3601 ggttaaagaa gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa
     3661 ctagagaagc tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat
     3721 aatagttttg catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg
     3781 aagtactcgt gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg
     3841 tgttctcagc tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa
     3901 agaaagtctc atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg
     3961 ctacccgttc agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg
     4021 caacttttgt tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag
     4081 tcatattagg cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg
     4141 tataataatt gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa
     4201 tctgcttgtt agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat
     4261 ctaaaggctt caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa
     4321 taatcaataa tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt
     4381 tacgcagatt tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt
     4441 tatctttact tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc
     4501 gtaactttat tcattataca tgatatttga attggcgtga atgattttat ttaaatattc
     4561 tatcaaaaat gtaagtagta aatccattac tataagatta aaataaatgt tatcaagata
     4621 caagctattt tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg
     4681 aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg
     4741 tttagcctta gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa
     4801 cactctcaga tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat
     4861 gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca
     4921 atttacgtaa agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa
     4981 ctcacgcaac aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca
     5041 ttgactactc ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag
     5101 ctggctcaat tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg
     5161 tatatgcgct caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa
     5221 gtcgttcaat ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat
     5281 tcattgaact ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa
     5341 aaattattga ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac
     5401 caagtcaaaa attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac
     5461 tttaaaccac tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc
     5521 agtatgtccg tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt
     5581 tccaaagact tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt
     5641 ggggtatata gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca
     5701 tctgctatat ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct
     5761 gacaaaacta tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa
     5821 ggaactacta agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg
     5881 aatagcattt gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct
     5941 aaaaatcaat ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct
     6001 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacattat ttgacaaagg
     6061 gcctattaaa tctaaaatag tatctttaga ttgctaaaat atgtgctagc aaatcattca
     6121 tggcttgttc ttatttttaa ttttatgcaa taaaaaagaa gaacaattag cgtgattata
     6181 gaaataaata atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt
     6241 attccacttt cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt
     6301 tttgaattaa cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg
     6361 ccttttgaag atttaagcgt aaatgtaatt tcatctttat ttggagaaaa gctactataa
     6421 ataacttgac catcttctgt aacttgatgg tttttcaaaa gcaattgatc ttttttatag
     6481 cctataggta cataatcaaa attctcaaat tcttcaagtt tattttccat ttgttcatta
     6541 gaataagttc cttgaaaatt catttctttc accatgttaa ctactgatga ataatttaaa
     6601 attgaaatag ctaatattaa taatagtata aaatttcttc tccttgtata agaaaatttt
     6661 tccaactgtg gagaaataaa tagagatact gaaaaagata tgaacaatgt tgaaaatatc
     6721 ataaaacgcc aaggagcttg aataatattt aaaggagtat tttgtaaggc aaaccatgga
     6781 aatatttttg tacaaagtag aatagatatt atacccatta tataagtgat tttagataag
     6841 attcctaatt ttttaaagtt aaatgttagt ataattaaac aaataagcaa agttaagcca
     6901 ataccaaatg atagtaattc tgaatctata cttccaatca aaaactcttt acccagataa
     6961 attgctcttt tttgtaatat atccacataa ggtctattca gatcttgata caatgtctgc
     7021 tcaatcatag gacctataaa cgctagtgaa agaagtaaag taactatagc agcttttatt
     7081 aatttaataa ttaagaagat ttctattttc tttttccgat aaaattgaat aattaaagca
     7141 attataatca ttaccatagt catagctaca gaaagaacat gagtgtaaat taataaagtc
     7201 atgctaatcg ccaacataac ccaagattct ttttttgagt cgtaaagctt tataaacccg
     7261 agtaaaataa gaggaagtat tgacattgcg agtaattccc ccattgcccc tcgaagaaat
     7321 atttctacac tcctaaaaga agatgttgta tatataatag cgaaaaagga gctagtaaca
     7381 tgttttttgg taatttcata cattacataa tgacatataa atagagtaat gagcgtaaca
     7441 atataaagaa aaaggttata gcctattgct aaattagtaa acttgatcaa taaaaataaa
     7501 ggatatagag ttaaccatgg gtaaaaatta tttacttgca tgccatatga tctaaacgtt
     7561 tcaaaattaa taggggagct aaaaatagat tctaaactta acgcacgatt aatgtgaaat
     7621 gccgtatcat aaaagccaaa tatttccatg tttttaaatg ctggaaataa atataataaa
     7681 ctaacaaatg cataaactat aaatattttt acagaaaaaa tttttttcaa attcatatac
     7741 cacctcatgt ttttttatat tacatatagt atcatttatt tttttaaaac actagattag
     7801 tcactatata aaatgtgatt aattttatta ttaataaaat attttttata ttacaaaagt
     7861 agtgttt
//
LOCUS       DAFELO010000091         7760 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFELO010000091
VERSION     DAFELO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.096"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="LP452_002544"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="LP452_002544"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL2015222.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="LP452_002545"
     CDS             complement(2032..3285)
                     /locus_tag="LP452_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL2015223.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="LP452_002546"
     CDS             complement(3291..4214)
                     /locus_tag="LP452_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL2015224.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="LP452_002547"
     CDS             complement(4435..6927)
                     /locus_tag="LP452_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL2015225.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="LP452_002548"
     CDS             complement(6934..7401)
                     /locus_tag="LP452_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL2015226.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="LP452_002549"
     tRNA            complement(7537..7610)
                     /locus_tag="LP452_002549"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="LP452_002550"
     tRNA            complement(7668..7741)
                     /locus_tag="LP452_002550"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAFELO010000092         7690 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFELO010000092
VERSION     DAFELO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.097"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            546..1109
                     /locus_tag="LP452_002551"
     CDS             546..1109
                     /locus_tag="LP452_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015227.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1388..1675)
                     /locus_tag="LP452_002552"
     CDS             complement(1388..1675)
                     /locus_tag="LP452_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2015228.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1665..1994)
                     /locus_tag="LP452_002553"
     CDS             complement(1665..1994)
                     /locus_tag="LP452_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2015229.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2299..2628
                     /locus_tag="LP452_002554"
     CDS             2299..2628
                     /locus_tag="LP452_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015230.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LP452_002555"
     CDS             complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LP452_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL2015231.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3553..4464)
                     /locus_tag="LP452_002556"
     CDS             complement(3553..4464)
                     /locus_tag="LP452_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL2015232.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4892..5500)
                     /locus_tag="LP452_002557"
     CDS             complement(4892..5500)
                     /locus_tag="LP452_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL2015233.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5506..6537)
                     /gene="vanA"
                     /locus_tag="LP452_002558"
     CDS             complement(5506..6537)
                     /gene="vanA"
                     /locus_tag="LP452_002558"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL2015234.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6530..7498)
                     /locus_tag="LP452_002559"
     CDS             complement(6530..7498)
                     /locus_tag="LP452_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL2015235.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2348 a         1531 c         1278 g         2533 t
ORIGIN      
        1 agttaaaact tttttagcag attttaaagt ttcgtcaaaa ctaatatatt ctggtagctg
       61 agcattcaaa tagctttcag acttcagaag aaaattacga gcatttttat agtccatctc
      121 taataaagtt attctttttt tatccaatac attcagctca attccttata atattctatt
      181 ccttagtatc tcaacattat ttactttata aaacgaataa attttgtagt tctaattcaa
      241 atattgcatg ttatatctaa tatgggagca ttttataatt ttcattgtga taaactcgtt
      301 attaacttta ggatacacta gttaggatta aattaggtat ctagaaattt acttaagatt
      361 tttttgttta tttcgtatac acactcgtat caaaaacaat cgattatgag acaaaatgaa
      421 atgacctctt ttattaaata aaagaggtca tattttgtat cataacttgt tgcattaact
      481 atgtatctat aactcccttt tatgatacaa tcaagccaga attaaagtac gaaaggaaga
      541 aaagcatgag tggatacatt tatggttatg cccgagtgag tacacgttca caagaactaa
      601 atcgtcaatt agacttactc aatgaacaaa attgtaatga aatcctaact gagaaaatga
      661 cgggaaccaa agcggttcgt ccccaactaa atcgtttaaa agataaaatt cgtccgggag
      721 atacggtaat tgtagaaagt ttttctcgat tgggtcgtag tacaaaggat ttgattgacc
      781 ttgttaccta ttttgaagaa caccatgtta agctggtgag cttgaaagaa aacttcgata
      841 ccagcacgcc tcacggacgc ttaatgatga ctgtcttcca agcatttagt cagttcgaac
      901 gtgatttaat tgttgaacgt accaaagaag gtttgaaaag tgcacgtgcg agaggtcgta
      961 aaggtggccg tccacgagtg aaccagaaag atgttgatcg agcagtaaaa ttgtataaaa
     1021 gtcaggttta tagcgtaaaa gaaattacgg aaatgactgg tattagcaag gcaacactct
     1081 accgctactt aaaagacaat agggaataat ctattttaat ctttaggaat ctataatatt
     1141 tatgggagaa ttagagtgat ttttttatta ttactaaaaa acaatataaa ataacgcatt
     1201 ttaagccctc tttgtgcata aggatgaagc ttttaatttg aaagaggctt aaaatgcgtt
     1261 ttaatttttt ctatttttca taaatatata ggttagtggt ttaaaattat agggtattac
     1321 aactctcagc tgttaaaaat tatcttttaa agtgtcgtct atcctctcaa gttgaaacat
     1381 tgggttctta cctaatcttt tttaattgtt cataaaaatt ttcatgcgtt ccacatagta
     1441 agataggtat cactgcatta tctttaattt catatgcaac gcgatatgtc gtacctgcat
     1501 acttgaatcc aatagcccaa attcctgata aatcacccgt tttttgttct cccgaatgag
     1561 gacgaactgc tatctcatca taaattatcg ttaaaaattg ttcttttaat gttttctctt
     1621 ttaatttttt tagatacttt attacgtgtt tagaaggtag aatctcataa gccaatttct
     1681 gctgccaact cctcttttgt catcggttta gaatttgcaa cagtatcttc agcaatagat
     1741 ttaaatgcag aagttaacgc attttttcta tttctaaatt cttttaagat ctcttgtttg
     1801 ctataacctt ctgaaagaat atcagccaaa atatcttcac taaaatcaaa aaattctttt
     1861 ttaggctcta caaactcata aaggatacca ttctctacaa gcttagcttc tacttcaaca
     1921 ccattgggca catcgaattc tttagggacc gttaacataa tagaatttcc ttgttttctt
     1981 gtttttgtaa tcatattatt tcctccatta ttttacattt cgtaattact tagtaattac
     2041 attgtacgct atttttagta gaaatacaaa ataataaaac tcctttagaa caattttcaa
     2101 aggagtttta tttgattagg aaattatgta gaataataat atgtttttaa cttggaatca
     2161 aaaacgcact tgcaagtaag tttacttcaa tacatgaaat tgcttataat agtgtattgg
     2221 aattaaggta gattgacaaa agagtgatca aataggtata cttcattcaa ataatcgttt
     2281 gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga ctgtcgtaca taaagaaaaa
     2341 gtaaatggaa taaaaaataa attggatcaa aaagattttt caaacttact tgtgttaggg
     2401 aaatgtttca gcgatttttc tagaattaaa attttctatg ctttagaaac ctataaggaa
     2461 atgtgcgtct gtgatttagc agaaatactt actgctagtg tcgctacaac gtcacaccat
     2521 ttacgttttt taaaaaaaca tggaatggca aaatcacgtc aagatggaaa agtagtttat
     2581 tactctttag cgaatgaaga catcctttcc gctattcgtg ttttttaaag ttatcagaaa
     2641 atctatctat gaaatcttag tttgggggta ccgtcaggaa aatgcggatt tacaacgtta
     2701 aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg
     2761 ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct
     2821 acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg
     2881 tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg
     2941 tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta
     3001 attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc
     3061 caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa
     3121 ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt
     3181 ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca
     3241 tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat
     3301 taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta
     3361 aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt
     3421 ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa
     3481 agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc
     3541 attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat
     3601 tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt
     3661 ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga
     3721 aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt
     3781 cataatcgca ctatgtggta aaccaacata gcgaatatgc catggttcat attgaattcc
     3841 tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc
     3901 ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag
     3961 tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc
     4021 ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact
     4081 aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga
     4141 catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa
     4201 attcacgata tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag
     4261 cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt
     4321 cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc
     4381 ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa
     4441 caataaaaaa aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat
     4501 gaaatatttc tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt
     4561 gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag
     4621 atgaattttc ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa
     4681 tgggcggtgt gaaataccac ccctctatgc gatcagtctt attatcaggc tatcttgccg
     4741 cagcgcacgc agataatgac cgcaatatcg ctctgcctta catcttcaat actatctact
     4801 atatataaat tccctaagaa ccagttacat acgtcgggtt tcctgccgaa agagttagct
     4861 tatatagttt gtccgtgcaa cggttaaaag tttatttaac ggggaaatca aaatagctat
     4921 tggggtatgg ttcgtctctt aatacatagt gccaccattc gaggctatat gcttcaaacc
     4981 cactgttttc catgatggag cgcaaacgtc tgcgattttg cgcttcattg catgatattc
     5041 catttgccgc atgatgagag cgttcatcca taaaatcaaa tcggctcccc attggtacaa
     5101 gctcacccgt gtctaatcga taaagcgtaa gatcaatggc actgccgcgg ctatggcttg
     5161 attttgaagc cacgtatcct tttgaaatca tctcagttcg gtcaatattg ggataataac
     5221 tttcctttgt caggttattt tccggctgtg cagcccattg cataaaacag tttacagcac
     5281 gattaggacg gtaaccgtcc catagaagca atccgtaccc ttgggtagca gccagttctt
     5341 ttgccttcaa aagcgattca gccaactcgt atgtccctac aatgcgattt acttcataac
     5401 cgtcaaccgg ttttccggtg aaattatccc aagtggcata tttagcgtcc caacgaacac
     5461 cgtgtactat ttcatctaaa aaagtaaatc ctatttccat gcttatcacc cctttaacgc
     5521 taatacgatc aagcggtcaa tcagttcggg aagtgcaata cctgcagcgg ccatcatacg
     5581 gggataacga ctgtatgacg tgaaaccggg cagagtattg acttcgttca gtacaatgcg
     5641 gccgttatct tgtaaaaaca tatccacacg ggctagacct ctacagccga gcgctttata
     5701 tatttttttt gccgtttcct gtatccgtcc tcgctcctct gctgaaaggt ctgcgggaac
     5761 ggttataact gcgttttcag agcctttttc cggctcgact tcctgatgaa tacgaaagat
     5821 tccgtactgc agcctgattt ggtccacctc gccaacagct aacgcggcac tgtttcccaa
     5881 taccgcacaa ccgacctcac agcccgaaac agcctgctca attaagattt tgctgtcata
     5941 ttgtcttgcc gattcaattg cgtagtccaa ttcgtccgcg ctattgactt ttttcacacc
     6001 gaaggatgag cctgaacgcg ccggcttaac aaaaacagga taggtaaacg tagctgccac
     6061 cggcctatca tctttattaa taacccaaaa ggcgggagta gctatcccag catttttcgc
     6121 aacgatgtat gtcaacgatt tgtccataca aattgctgag ctttgaatat cgcagcctac
     6181 aaaagggata ccggacaatt caaacagacc ttgtatggat ccatcttcac ctgacttgcc
     6241 atgcaaagct gaaaatgcta catcaacatg gttgatttca tattcatggt tctttttaac
     6301 aagtaatccg tgcatttttt tatccggcga gagtacagct gaatagcaat tgtcgttttc
     6361 ccattccgcg caaggttttt cgcacatttt ccatacacca gatttcgtaa ttccaatgta
     6421 taacggctcg tatttttctt tattaatgtt agcggctatc tctattgcag attttaccga
     6481 tacgtcatgc tcctctgagc aacccccaaa cagtattgca acttttattc tattcatgct
     6541 cctgtctcct ttcaaaatcc aaacagtttt taatggtttt ttcaacggta tcacgcaacg
     6601 cttgctcggt ataataggcc gtatgcggtg tgattatcac gttaggcatt ctttgaagtt
     6661 taagtaaaaa ttgattatca attggttttt gggtgcaatc agagtagaaa aactcttcct
     6721 ctccttccaa tacatccaat gcggcaccgc ccagtttccc gttttctaat gctttaacca
     6781 actcataggt atctacaagt ggaccgcgcc cagtattgat aagaaatgct ccttgcttca
     6841 ttctctgtat ttgttcgtgg ctgataatat agtgcgtatc cgtattgagc ggcacatgaa
     6901 gcgtaacgat atcgctattt tgcagcaact catcaaacgg tacatagttt acctctatac
     6961 ttcggctgcg actataagcc aacactttac atccaaatcc tcgcagccgc tcaataaccg
     7021 ctttgcctat ctggcccgtt cccaccacac caactgtcat gtcgctgagt accttgccac
     7081 ggtcgctgtc caacctgaaa tcatgttttt ccacagagcg cacaatcgat tttacgttgc
     7141 gtactgccat aagaattagc atcatagtat aatcggcaac gctatccggc gagtacgcca
     7201 cattgtcgac agtgatgccc attctcttag cagcagttgt atctatatga ttgcagccga
     7261 tgcttcgggt agaaatatat ttcacaccgg ctctcttcag cgcaagaaga atagaggcgg
     7321 aaatctctga tttatgtccc acactgatac attgattgaa aggcgcggat ttggcgttgg
     7381 attccgacac gttggcgtta attatcgttg ccataacgcc aaagcgaggc gaaagagcat
     7441 ggaatgcatc tgcctcatcc tgctcacatc cataaacagt aatgccgatg ttattcataa
     7501 ttaagaccaa ccctttctgt gaaaggcacc aaataaaacg gggatgccaa tggtaatctt
     7561 gtcaaatctt tctctgaaga acgaaaacgg ctcgttctta cggtattaag tctaccagcc
     7621 gtttcttaat attaaattgt taatttccta agaaaaagta aagataacct tgagattttc
     7681 ctaaaaaatt
//
LOCUS       DAFELO010000093         7625 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFELO010000093
VERSION     DAFELO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.098"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(14..706)
                     /locus_tag="LP452_002560"
     CDS             complement(14..706)
                     /locus_tag="LP452_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2015236.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LP452_002561"
     CDS             complement(1143..1712)
                     /locus_tag="LP452_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2015237.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LP452_002562"
     CDS             complement(1786..2109)
                     /locus_tag="LP452_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015238.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LP452_002563"
     CDS             complement(2263..3075)
                     /locus_tag="LP452_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015239.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LP452_002564"
     CDS             3429..4277
                     /locus_tag="LP452_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL2015240.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LP452_002565"
     CDS             4422..5363
                     /locus_tag="LP452_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL2015241.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LP452_002566"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LP452_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DAFELO010000094         7595 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFELO010000094
VERSION     DAFELO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.099"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(939..1352)
                     /locus_tag="LP452_002567"
     CDS             complement(939..1352)
                     /locus_tag="LP452_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL2015242.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="LP452_002568"
     CDS             complement(1808..2293)
                     /locus_tag="LP452_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015243.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2677)
                     /locus_tag="LP452_002569"
     CDS             complement(2432..2677)
                     /locus_tag="LP452_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL2015244.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2870..3670
                     /locus_tag="LP452_002570"
     CDS             2870..3670
                     /locus_tag="LP452_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2015245.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3689..5557
                     /locus_tag="LP452_002571"
     CDS             3689..5557
                     /locus_tag="LP452_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2015246.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNAVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5550..6041
                     /locus_tag="LP452_002572"
     CDS             5550..6041
                     /locus_tag="LP452_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL2015247.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6029..6583
                     /gene="srlA"
                     /locus_tag="LP452_002573"
     CDS             6029..6583
                     /gene="srlA"
                     /locus_tag="LP452_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL2015248.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6601..>7595
                     /locus_tag="LP452_002574"
     CDS             6601..>7595
                     /locus_tag="LP452_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL2015249.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1441 g         2233 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatatag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaagt
     2641 tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat
     2701 tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc
     2761 tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta
     2821 gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg
     2881 gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag
     2941 tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg
     3001 ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat
     3061 agaagaaggg atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa
     3121 tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga
     3181 attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat
     3241 gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc
     3301 ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc
     3361 gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt
     3421 aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc
     3481 tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag
     3541 tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta
     3601 tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac
     3661 aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt
     3721 gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac
     3781 cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc
     3841 aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga
     3901 tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat
     3961 cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc
     4021 tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat
     4081 gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg
     4141 agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac
     4201 gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa
     4261 tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat
     4321 ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat
     4381 ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca
     4441 atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat
     4501 aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg
     4561 attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc
     4621 tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat
     4681 agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc
     4741 tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt
     4801 agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac
     4861 aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat
     4921 acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc
     4981 tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac
     5041 taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt
     5101 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     5161 agaatattta aacaaattag tcgctccttt gatcagaggc tattatgtgg atgaagaatt
     5221 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     5281 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     5341 tgatcaaggt ttagcagaaa caggaggaaa tgcagttatt tttttatttg ccatacctga
     5401 agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact
     5461 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5521 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5581 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5641 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5701 tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca
     5761 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5821 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5881 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5941 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     6001 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     6061 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     6121 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     6181 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     6241 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     6301 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6361 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6421 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6481 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6541 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6601 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6661 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6721 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6781 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6841 atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca
     6901 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6961 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     7021 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     7081 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     7141 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     7201 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     7261 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     7321 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7381 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7441 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7501 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7561 attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAFELO010000095         7317 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFELO010000095
VERSION     DAFELO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.100"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(462..887)
                     /locus_tag="LP452_002575"
     CDS             complement(462..887)
                     /locus_tag="LP452_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015250.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1261..2232
                     /locus_tag="LP452_002576"
     CDS             1261..2232
                     /locus_tag="LP452_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2015251.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2264..3661
                     /locus_tag="LP452_002577"
     CDS             2264..3661
                     /locus_tag="LP452_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2015252.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3820..4443
                     /locus_tag="LP452_002578"
     CDS             3820..4443
                     /locus_tag="LP452_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015253.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            5192..7285
                     /locus_tag="LP452_002579"
     CDS             5192..7285
                     /locus_tag="LP452_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2015254.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         2492 a         1226 c         1484 g         2115 t
ORIGIN      
        1 aaaaattact acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat
       61 atttgaaata aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt
      121 ttactgatga agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat
      181 agtgagtttt gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat
      241 aagattcaaa tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca
      301 agcttccttg gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat
      361 caggactatc gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca
      421 gaaaagaagg ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg
      481 ccatcctcat ccttcaaacg gtaattaccg atttttgtaa atatgactgt tgtttctttt
      541 ttattttctt ttcctgaaat aatatattcg tcatctttgg tagtgatctt gatatctgtc
      601 agcactgttt tttcttcaga tgattcagaa gaatcagttc cttgtgtctg atacgtgcgt
      661 gtccattcca tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta
      721 cgtactttta gcgtatatac actgttggtg gtatctgctt tttccgcttt taagacagaa
      781 gaatcggttg tttcgtccga ggattggtca gagccgccaa acaaatcaca gccagtcaat
      841 gtcaaaagtc ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca
      901 tttgatagga taaagaaatt ataccattgg aatgtaaaac ttcaaacgga tggaagagaa
      961 aaacgcacaa aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa
     1021 aatccgccac tttcagaaag cgttttaaaa aagtgttgca atcttttaaa aaaatgctat
     1081 attaaaaatg aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc
     1141 tctactgaag actttagtta tatggatatt gatgacaagc aacactatgc aaagaatgcg
     1201 gtcatcataa tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt
     1261 atggcatcga tccgtgatgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt
     1321 tacttgaatg gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca
     1381 gcgttggact ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct
     1441 attggacttt tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc
     1501 gaaagagtag ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca
     1561 gaaaaaattg aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc
     1621 ttcttatccg aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt
     1681 atcgcattga attgtccaga aggaccagca ggtattgata caatattagt gaatgatcga
     1741 gaaagtgtag caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt
     1801 attgctgcca ctcaaacaga ctacgtagca agagaacgtt tagcaggagc caaagaagcg
     1861 gtccaaaatg atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagc
     1921 ggctattacg gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat
     1981 tacaatatgt cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag
     2041 gatattgcgt tcagtcacta tgattattta gataaaaacg atctgagtat ccttccgaaa
     2101 atcacgatca cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttaaaa
     2161 cgaatcaaaa aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat
     2221 ggaatcaact aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt
     2281 tccagcaaat ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc
     2341 aggcgatgga aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt
     2401 ctttgatcaa gtaggacctg aaaaaacctc gcaagtttat acgaagtatc aagaggatgt
     2461 acagttgatg aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgttt
     2521 gatacctgat gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt
     2581 tgatgagttg attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc
     2641 gatgtatctg caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa
     2701 atatgccaaa agttgttttg aattgtttgg ttcgaaagta aaaaaatggt ttactcataa
     2761 tgaaccaatc gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc
     2821 agatatgaaa aaagcgatcc aagttggcta tcatgaagcg ttggcttcag cgttagccat
     2881 caaatcttac catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc
     2941 aagctatcca agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc
     3001 gttcttcaat cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat
     3061 tgagatagta aaagaacttg atctcatgcc tgtgatccat gcagaggatt tgacggttat
     3121 caaagaaaat acagtagatc ttttaggtat caattattac cagccacgcc gggtaaaagc
     3181 aaaagaaaca ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta
     3241 tgaaatgcct ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat
     3301 ctatgatatt ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga
     3361 aaacggtatg ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga
     3421 tgattatcgg atcgaatttg tccaatctca tctgaaatat gtccatcaag ccattcaaga
     3481 aggtgccaat tgtttaggct atcatatgtg gacatgtatg gataactggt cgtggaccaa
     3541 tgcatataag aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac
     3601 aatcaaaaaa tcgggtcatt ggttcaaaga cgtatcagat aaaaacggat ttgaggctta
     3661 agaaagaata gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag
     3721 aagttcaatt atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata
     3781 taatagaata gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt
     3841 cttctctttt ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga
     3901 aaaatgacag cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca
     3961 gtagataatt tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc
     4021 aattacgaaa aaggcgaatt ggacatttat ttgccaaaag gaaaagaaga aaatcaattt
     4081 attaatatcg atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag
     4141 catttgaccg ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga
     4201 aaagtgatca ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg
     4261 gaatggaaaa agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag
     4321 tcgacaggaa caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa
     4381 aatcaagcct caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa
     4441 tgaaaaacag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggcaa
     4501 aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
     4561 cgtgtatcaa gaggtcttga gcctgccgtt taactctgag ccacaaggag caatttcaaa
     4621 aaatagctcc ttatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
     4681 caccgtttac caagaggttg taaaattagc gtcctgacat aatctaatac cgagggtcag
     4741 ctgattgaac accgtgtatc aagaggtctt gagcctgccg tttaactctg agccacaagg
     4801 aacaatttca aaaaacagct tctcatgttt tataaatatt gtgacttgtg gccgaggagt
     4861 tggcaggaga acaccgttta aggaagaagt tgtgaaaaag ctgtcctgca tcaagaaata
     4921 aggagctgcg acaagaattt tgtcacagcc tctttttttg tacgaatacc gagaaactgg
     4981 cttttgaata ccgtggagtg aagcggtcac cacaagactt ttatctcttc attagtttaa
     5041 tcgcttacat cgcagcggtt ttatgctgtg aaaagtggac ggaataatct ttagaaagaa
     5101 taaaaaaatc ttaagataac aactttttta ggaaattttc atttttcagg agcaaaataa
     5161 gaaaaaatgt gaatagagag aagtgatata cgtgacacaa cttaaaaaat tgtgccaaaa
     5221 tcgtttagca atcgttttga cagctgtttt ctttttctgg ctgaagacga tcattgccta
     5281 ctatgcagat ttttcattag gtgtggaagg tacgatccag tactttatct tgtggatcaa
     5341 cccaattgcg actaccttat tgttttttgg cctttctttg tatattaaaa agccaaagcc
     5401 agcgttagct attcttttga tcattgatat tttaaatacg cttttgttgt acttgaatat
     5461 tattttttat cgggaattca cagatttcat tacagtcaaa tctgtattag gcttttcgaa
     5521 agtgtcgcaa ggattgtcag gaagctcctt ttctttgatg aagccccatg atgtcatcta
     5581 ctggctggat atcgctgtgt tcattgggct actggtttgg ttgaaggtca agaaaatacc
     5641 tatcaaaagc aaccctgtcg gcaaaccgat ggctatcgct gttacctgtc tttcagggct
     5701 tattttttca ggaaatctag cgctgagcga agccaaccgt ccacaattgc tgcagcgaac
     5761 atttgaccgt tcatatattg tcaaatatct aggaatcgat gcgtatacta tttatgacgg
     5821 aatcaaaaca ggaatgacta gcagtgttcg cgcgcatgca agcagtaatg ggattgatga
     5881 agttttagat tatacaaaga aacattatgc agagccaaat cccgaaacat ttgggatcgc
     5941 taaagggaaa aatgtgatcg ttctccattt agaaagtttc cagcagtttt tgattaatat
     6001 gaaggtagac ggacaagaag tcacaccgtt tttaaatagt attttccaga atcaagcaac
     6061 gatcagcttt gacaatttct ttcatgaagt aggacaggga aagacaagtg atgctgaaaa
     6121 tatgttggaa actggcacat ttggtttacc tcaaggctca ttattcacag aacttgggtc
     6181 agacaatgtc tttcaagccg caccagcgat ccttggacaa aagcaaggct atacaagcgc
     6241 tgttttccat gggaatgtag ctagtttttg gaatagagat catgtatata aaaacttagg
     6301 ttatgacaat ttctttgatc gatcttattt tgatgaatcc gacgaaacgt taggttatgg
     6361 gatattagat aaagatttat tcagagaatc tgcccaatat cttgagcatc tacaacagcc
     6421 gttttacacg aaatttcttt ccgtaaccaa ccacacgcct tattatacgg atgacaagaa
     6481 tttcgacttt ccatctttga atactggcaa tagcacagta gataattatg ttcgaacggc
     6541 acattatctg gatcaatcat tggaacaatt ttttacgtat ttgaaaaaat ctggtattta
     6601 ccaaaattca atctttgtga tttacgggga tcattttggg atctcgaata cagataataa
     6661 agatttagca agcgctcttg gtaaagatcc agatacatgg gatgaatttg acaacgcaca
     6721 gatgcaacga gtgccattga tgatccatat gcctggttat actaaaggga cagtcaatca
     6781 tgaatacggt ggagaaatcg atgttttgcc gacattacta catctattgg gaatcgatga
     6841 taaagattat cttcattttg gtacagactt attatcttct cagcatgatc aagtagttgc
     6901 ttttcgaaat gggaactttg tgacacccaa atacaccgtc ctaggaggaa aagcttacaa
     6961 taatcagaca ggagaaatca tcgatacgaa agcagcagga atcgatgagg aaatctccaa
     7021 agatcaggaa aaagtcaaga ctgcgttaag tctttcagat aagctgaatc aggaaaatct
     7081 cttgcggttt tatgtgccaa acggatttga gacgattgat ccgaaaaaat atgactataa
     7141 aaatgaagcg gaacgtaatg aagcaatcga aaagcaaaaa ggcgaacatt cgacaagtgt
     7201 cttttcaaaa aatggtaata aaacaacaac caacttatat ccagtaacaa gaacagacgt
     7261 tgaacagcaa aaaaataatc aataaacaat caaaccaacc ttcagtagtt agacaac
//
LOCUS       DAFELO010000096         6901 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFELO010000096
VERSION     DAFELO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.101"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            1..345
                     /locus_tag="LP452_002580"
                     /pseudo
     CDS             1..345
                     /locus_tag="LP452_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            361..510
                     /locus_tag="LP452_002581"
     CDS             361..510
                     /locus_tag="LP452_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015255.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            591..1187
                     /locus_tag="LP452_002582"
     CDS             591..1187
                     /locus_tag="LP452_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL2015256.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1200..2099
                     /locus_tag="LP452_002583"
     CDS             1200..2099
                     /locus_tag="LP452_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL2015257.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2102..2233
                     /gene="ssb"
                     /locus_tag="LP452_002584"
     CDS             2102..2233
                     /gene="ssb"
                     /locus_tag="LP452_002584"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2015258.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2255..2587
                     /gene="ssb"
                     /locus_tag="LP452_002585"
     CDS             2255..2587
                     /gene="ssb"
                     /locus_tag="LP452_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2015259.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2632..2865
                     /locus_tag="LP452_002586"
     CDS             2632..2865
                     /locus_tag="LP452_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015260.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3024..3146)
                     /locus_tag="LP452_002587"
     CDS             complement(3024..3146)
                     /locus_tag="LP452_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL2015261.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3336..3560
                     /locus_tag="LP452_002588"
     CDS             3336..3560
                     /locus_tag="LP452_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015262.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4003..4209
                     /locus_tag="LP452_002589"
     CDS             4003..4209
                     /locus_tag="LP452_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2015263.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4209..4460
                     /locus_tag="LP452_002590"
     CDS             4209..4460
                     /locus_tag="LP452_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015264.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4476..4631
                     /locus_tag="LP452_002591"
     CDS             4476..4631
                     /locus_tag="LP452_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015265.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            4641..4913
                     /locus_tag="LP452_002592"
     CDS             4641..4913
                     /locus_tag="LP452_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015266.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5358..5537
                     /locus_tag="LP452_002593"
     CDS             5358..5537
                     /locus_tag="LP452_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015267.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5596..5952
                     /locus_tag="LP452_002594"
     CDS             5596..5952
                     /locus_tag="LP452_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015268.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         2469 a         1114 c         1369 g         1949 t
ORIGIN      
        1 ccacttttct agccaatatc caaaaatttg tctctcactt attggaagtc gtaccagggc
       61 aaatccaatc cactgatttt ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc
      121 aagaagccgc gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa gtcttactct
      181 atcaaaaggt ggcagcttgt acgtctgaag cctgtgattt taaaaggtca gaagggttca
      241 tttgaagcaa cgattgtttt aaaagaagac ttcactacag gctttgaatt ttctgaaaag
      301 aaaaaaacga acttcaagaa aagaacacgc cgcactgcaa aataatggag ggttaatctt
      361 atgcgtaata ccacacacaa acaacaagaa atcgaaaatc aaaaagccaa aattcgttca
      421 gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc aattgaagaa ccagcagagg
      481 attcaaaaag aaatcgaatt agatcgataa aatcttccta actattgtat acgtataaac
      541 ttatgttata ctgtttttat aagtttatac gtatacaaag gagggagaaa atgacaaaag
      601 caatggtata caagctgcta gaagaattta atggaacttt ggctttgaag gatgccaaaa
      661 aagcgggcat ttctcctgta acgatcaaac gtatggtaga tcgaggggag ctggatcgag
      721 agtatccagg ttttttcacc ttacctggtc aatttcctga tgaattgttt atggcgcaaa
      781 aaaaatatga acgcggcatt atttcacata ttaccgcgtt agatttgtat gatttaacag
      841 atatgatccc tagacagatc gatctgactg taccttacgg ttatcacgtt tctgaaaaag
      901 gattgaagga attcgcagtt gagctacact atacaaaagc ggaatggtat gaattaggta
      961 agattgaaat aaaaagtcgc tatgggaatc ctattatagc ttatgatcca gaaaaaacct
     1021 tgtgtgacat ttggaatccc tggtataacg ttgaggatga aatcaaagta aaagcaatca
     1081 aaaactacat ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt
     1141 tgcctacaga taaaacgatg cgctcttata ttatggcttt aaattaaagg agaatacaca
     1201 tgacacctac gcaattaaaa gcaaaagtga agaatatttc tagagaaaaa gaagttgatc
     1261 cgcagcttgt tttaagacat tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc
     1321 cttatcgtga aaattttgtg ttaaaaggcg gcttcttaat tggctcaaag tatggcattg
     1381 aaaatcgaac aactaaagac attgatacga ctttacggga aatgaaagta accaaagaaa
     1441 cattgacaac ggttctaaac gatatttttt caacgccgac gaaagaaggc attcaatttg
     1501 agattcaagg aatgaaagaa acgagggaag ctgattatta tccagggttt agcttacgtg
     1561 tactggcaca tttggaaaac atgcggccag attttaaggt agatgttact acaggtgact
     1621 cgatctatcc tgcgacgatc actcacagtc ataaattgat gtttgaggat cgaacgattc
     1681 ctttggaatc ctatcctaca gaacaaatta ttgctgaaaa attaagtgca acctttgatt
     1741 ttttaacaga taattcgaga ggaaaagatt tttatgatct ctatactatt cctaaaatgg
     1801 agaagatcga tgagaagacc ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa
     1861 aaactgatcc gttaaagata tactatgaaa aggatatgga tgtactgaaa aatagtcacg
     1921 agctaaaaga tacctggaag aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt
     1981 acgatgagac aatggattcg attagtaatt tgatggaaaa agtgattcac gtcgaagaaa
     2041 aagagcgaaa actagcttat caaagacacc aacagatgaa tagagaaatg gagagataaa
     2101 gatgattaat aatgtgacat tagtaggacg cttaaccaaa gatcctgatt tacgttatac
     2161 cgcaagtggt actgcagtag cgacttttac cctagccgtc aaccgaaatt tcacgaatca
     2221 aaacggcaat tgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac
     2281 aatggcgaca ttagctaaaa aaggtagctt gattggtgta gttgggcgga ttcaaacgcg
     2341 gacttacgac aaccagcaag gacaacgtgt atatgtgact gaagttgtag cagataactt
     2401 tcaactatta gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc aatcgtccac
     2461 cagtccaagc accgccacct ttgaacaacg gaatacagcg acgcctaaca acaatggatt
     2521 gaacgcttca cagaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc
     2581 gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa ttagtctgac gatggatcta
     2641 ttgatacccg taattgcatt tatagacggg atttctttgg gcgtcactga aaatcctttg
     2701 gtaaatagaa ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat
     2761 cttttaaaaa gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacagtagca
     2821 gcgacagctg ttatacttac ggctattcta ccgctttata tgtagagtta acgggaaatc
     2881 gttgaacttc ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgctaacaa
     2941 cccctcatta acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac
     3001 cgctgacctg ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc
     3061 aaactaatca aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat
     3121 gcggacaaaa ggcttactcc tttcatagat tttagcactt acattcataa gcaccaccac
     3181 ctttccagaa gtcgccaccg tcttcaaact ttactgtctt gactattata gcatatctaa
     3241 aatcgaataa aaattctcaa ttttagctgt ttttgctata attttagcga ggtgatcgtg
     3301 atggaacaat atattggttt actcgataaa ttagggtgat caagacacag ccattgcttg
     3361 tgcgatttac attacgcacg agtcatgaac caatcaattg tgtagtggcc aatatagaaa
     3421 tagcggataa attgctgatt ttggaagaag gcaagtatac tatttcagtg acaggtcact
     3481 tcaataaacg aaagcaacta gtcattgaat caatgcaaat tagaaaccct gatcatttta
     3541 cgcgatcgat gagcatataa aagaagagct gcagtcctaa atgaggattg tagctctttt
     3601 tgttttacat agaaacagta aaaatgaaaa ggcctgttta ggaaggcaca agcgccttct
     3661 caattgaaag acctttctct cactccaacc gccaccgccg ttcgagaaga aaggaagtga
     3721 acgctacgcg ttcactaaga taaagggtct tgcatagcaa gacaaaatag aaaaaatttc
     3781 ctctcaccaa aaggttcgct cctgttcgtt atctctttat tgatttgtca gaccggcaac
     3841 aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc
     3901 ttgtcttgtc gcttttcgtt gattcattct ggccaacaaa tcaataaaga aagaactaac
     3961 gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat
     4021 tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgtcgaa
     4081 tgcgattgtg gagaaagagc gaaatgtatc aaaagctatt gtttgtatca atgtccaaca
     4141 tgcgggaaaa aatatacctt gtcttatggc gattatattt taattgatga gaaaggaaag
     4201 aaacgctgat gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt caaattttgg
     4261 tggacaaaga agtcatttct gaaaaagtag cagaagtttt acttattccg cggtactgga
     4321 cgatagaaac ccctttagta gaggatgcac cgactatgct atttgaagac ctagtgaaga
     4381 ttgcaggata tgaactgcat tatcaagaaa acttggaaac ttatgaaaac gaatattggt
     4441 taaccaaatt agagaactaa aaaggagaaa aaaatatggc tcaaaattac tatgacgaat
     4501 ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg ttcctataca
     4561 acgaaacacg tgttccaaaa aaacattata aagaaaaatt gtcggtagct gtggaagaaa
     4621 tgattgaata aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca ctggaagaac
     4681 tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa gtaggtgtca
     4741 aaccaagcac tattaaacct tctgaatacc aagctttaga actaacgtat gcgaaattta
     4801 tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt gaaaatatca
     4861 ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa taaggtgatt
     4921 tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc aataatcatt
     4981 tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa agcctagtaa ctaaggaaat
     5041 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataaa
     5101 cgggcaaagc ccgataactt aagagaaaga aggtgatcca tttgaacaag attattccct
     5161 tattgttagt actgtttgtt ggcgaaatac ttatgcgaga tagtgcagct gtacagttaa
     5221 acctcgctgt atttttggta atactggtgt tttgtttgat cttttggcgg tttgtattag
     5281 tcctcgtgag tctactattc aagatgattg cagccatatt tttcataggg ttatttcgat
     5341 gaaaggagtg agtaccaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
     5401 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtatggcaaa agtgaatcta
     5461 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatgtac
     5521 cagcgctaaa aaagtgacgt cactttttaa tttgatctat ataagtgata tcacttttac
     5581 caaggaggga attaaatgtt aaaaactaaa aagaattcgt acaaattaaa gcttagtata
     5641 ctatctatta tttgtctttt aggcatgata attagtggtc atttgtatag agtggcatat
     5701 acgcagttta ttgagtacaa ggttcactct ttaggattag ctggggatcg atacgttgaa
     5761 tatgtccatt attcattaaa atgttcttca tatattcttc tttggcttat tttttcattg
     5821 tttgaattgc taaatttaat agcaatatct tcatatcact tagataagct aaataccaaa
     5881 tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt tagaaagtga cttaaacaag
     5941 ctaaaaaaat aaaaagaaga aaacataagt taactaaaat tattttatta agaatgatgg
     6001 aatataagga taaggttacc aacataacga tcattacttc tctgatatgc tatggacttc
     6061 tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc ttagttttgg
     6121 ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta tttgttttat
     6181 caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa gaactcaaag
     6241 aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga catactggta
     6301 ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat gaagaaaaaa
     6361 caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa gatggtgttg
     6421 ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa atgccttact
     6481 tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt gattttaggc
     6541 gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac atgaaactga
     6601 attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg attggattat
     6661 atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa tttaaccgat
     6721 taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc aacgaataaa
     6781 ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga tgatgtacta
     6841 tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt aaaggttttt
     6901 a
//
LOCUS       DAFELO010000097         6748 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFELO010000097
VERSION     DAFELO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.102"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(613..930)
                     /locus_tag="LP452_002595"
     CDS             complement(613..930)
                     /locus_tag="LP452_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2015269.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(931..1185)
                     /locus_tag="LP452_002596"
     CDS             complement(931..1185)
                     /locus_tag="LP452_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2015270.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1544..1948
                     /gene="tnpA"
                     /locus_tag="LP452_002597"
     CDS             1544..1948
                     /gene="tnpA"
                     /locus_tag="LP452_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL2015271.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1965..3113
                     /locus_tag="LP452_002598"
     CDS             1965..3113
                     /locus_tag="LP452_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2015272.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3336..3554)
                     /locus_tag="LP452_002599"
                     /pseudo
     CDS             complement(3336..3554)
                     /locus_tag="LP452_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3820..4014)
                     /locus_tag="LP452_002600"
     CDS             complement(3820..4014)
                     /locus_tag="LP452_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015273.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(4114..4200)
                     /locus_tag="LP452_002601"
                     /pseudo
     CDS             complement(4114..4200)
                     /locus_tag="LP452_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            5366..6406
                     /locus_tag="LP452_002602"
     CDS             5366..6406
                     /locus_tag="LP452_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2015274.1"
                     /translation="MTDFKFFKVDRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         2404 a         1048 c         1031 g         2265 t
ORIGIN      
        1 aatatatttt ttaccagcaa accatacata tcttttttca ttaagtatgt tcactatctc
       61 tcctccatta attatattta gcatcaaata gttattgtac ctatatttta tcccattcct
      121 tttaaaacaa gaatacgtac aaagtaaaat gttgtaaata gtctagcagg gaagtatatt
      181 ttcatgtacc aacataatat ttaagaagtt tcccatagat atattggact tcctctttta
      241 aaacacaaaa ataaatacta ccttatttag gtagtattta ttaaaagtta cgtcaatttt
      301 gattaaaaat atactacttg aacctctcat gagtaaagtc acgaaatttt tgggaataga
      361 gcgtacttga tatcttattt tttcactaat agcagtgtct cttattcacc aagcgatccc
      421 cgtagttcct aaggttctta atttttctct agactaatga ttgtatcttt agaccttcgg
      481 ccataacatg gagcaaaata aaaaggtact aagtcttata tatcaatctt cgagtaaccg
      541 acggttaaca accatatcga gcggcattaa tcacccacct gtagaggatg agcgactttt
      601 tcctaattat tgttaaaagc ctagtttcat ttgtttagat tggaaaatac gtcctacaag
      661 tatctctttt ttttcatggt cgatccgata aaataaggca tagcttttgc cgatcacgat
      721 ccgatatgtt gggatatcca ttccatagat ttcggaaact tcctcatgca tagttggaaa
      781 atttttggtt agttctaaag ccttctggat cgcacctaca aatcttttta tgctggctgg
      841 agaaagagca agctcatttt cccattcttc tattatcgtt tcaaggctgg tctgaaaact
      901 tttagaataa actagcgtgt actctttcat ctattcactc cagccgtatt ttccgaaagc
      961 ttttaccatt tcaatttctg aaactgttaa acctttctca aagtcttgga aactttcttc
     1021 aatcaatttg cgatcgtgtt cttttgctat ttgagtagta tctaatatga ataagttgtc
     1081 ttcttgtttg gcttggataa tgtctccgct ttttaatcca agagagcttg gaaggatcac
     1141 tcccacgcta ttgccgatct ttctaacctt tagttctttt cccatgttat cacactcctt
     1201 ttagtttttg atgaataaat tataacatgt tataacaaca attgccaacg gtgtttctta
     1261 agggattcca ctccatatta ttattggaca aaataagcca cttagaacgc tgaaaacata
     1321 tacaaacgat atcaaaatac gagtgaacta ctcaccatac acttccgtta acatccattt
     1381 aacagaatta ggcagaagtc gcccatcctc tataggtggg tgatgaatgc cgttcggtat
     1441 gagggttacc gttggtaact cgaagaccga caactaaggc gtattgcctt tttattttgt
     1501 cccatgttac aatcagtcta tacaagaaat gagttgatat aacatggaat tagatacaaa
     1561 taatcattca gtatttcttc tttattacca ccttgtttta gtaacgaaat atcgtagaca
     1621 agtgattgat gatgaaatat ctgattatgc caaaactact tttgaaagaa tttcagaatc
     1681 gtatcacatt actttagtgg agtggaatca tgataaagac cacgttcata ttatgttcaa
     1741 agctcaacct aaaacagaat tgacaaaatt catcaatgcc tataaaagtg ccagctcaag
     1801 attgataaaa cgtgactttc caagagtcaa acaatttctt tggaaagaaa tgttctggtc
     1861 taaaagcttt tgtcttctga caactggtgg cgcaccaatt gatgtgatta agaagtatat
     1921 ccaaaatcaa ggaaacaatc ataaatagaa agtaggtgaa ctttatggaa caactaaaag
     1981 catacaaatt cagaatctat ccaacagagg aacaagaaat cttctttgcc aaatcttttg
     2041 gttgtgtccg taaggtctac aatctaatgc ttgatgaccg aaaaaaggcg tatgaagaag
     2101 ttaaaaatga ttcttctaaa aaaatgactt ttccgacacc agctaaatac aagaaagaat
     2161 ttccgttttt gaaagaaatt gatagccttg ctttagccaa tgcccaactt aatttagata
     2221 aagcatacaa gaattttttt cgggataaat ccgttggatt tccacgtttc aaaagtaaga
     2281 aaaatcctgt tcagagttat acaacgaata atcaaaatgg tacaattgct ttgattgata
     2341 gtaaattcat caaagttcct aaattgaagt cattagtaag aattaagctt catagacaac
     2401 caaaagggat tatcaaatct gctacaatat cacgtcattc tagcggtaaa tactatattt
     2461 ctttgttgtg taaagaagaa attatcgaat tacctaaaac taattccaca attggtattg
     2521 acttaggtat tatggacttt gcgattcttt ctgatggaca aaaaattgat aataataaat
     2581 tcacgtccaa aatggaaaag aaactaaagc gtgaacaacg caaattgtct aggcgtgctt
     2641 tattggctaa aaataaaggt accaatctat ttgaagctaa aaattatcaa aaacaaaaac
     2701 gcaaagtagc tagattgcat gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt
     2761 tgagtacaga aatgatcaaa aatcacgata ttatctgtat tgaagactta aacacaaaag
     2821 gtatgttgcg aaatcataaa ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg
     2881 tgactaaatt acaatacaag gctgactggt atggacgaaa aatcatcaaa gtagataaat
     2941 ggtttccgtc tagtcagatt tgttcagagt gcggacataa agatggcaag aaatctcttg
     3001 aaattcgaga atggacttgt cctgtttgtc atactcatca tgatcgtgat atcaatgcta
     3061 gtatcaatat tctgaccgaa ggtctaagac tacattctat gggattggct tagatccaat
     3121 catcaagaac cgtaggaact acggggatag cttggtaaat aagagacacc gctgttagta
     3181 aagaaatacg ctatcaagta tgctctattc ccaagaagct cccacttcaa gcgttaagaa
     3241 ccgtcaggtt tagctaagtg gtgagtagtt cacgtaggta tttttattat atatagattt
     3301 aacatatgtc ggtcggttga ttgatatagc taaagttata actcaaccga agccgattca
     3361 tctacacgaa taaagttgcg agtgttctcg gctaattcat aaaaattgtt atgtgttcca
     3421 tatagcaata ctggaataac tttattttct tgaatctcat aagcaactct atacacggtg
     3481 accgcatact taaatccatt agaccaatta cctgataaac ctccagtttt ttgctcttac
     3541 ttaaatggat caatggcaat ttcatcacaa acctatagag gatgagtgac ttctgcctaa
     3601 ttacgttaaa aaatattgaa agtaactcta tgtagcaaaa aatatctaga atttcatttc
     3661 ctcaaaactg atcgatattg tatctaaaaa tatatactac ctaaaatagg tagtatatat
     3721 taaaatatgt gcttttgtta gaaaaatcaa tacataaatt atcatatctg tatatgtcat
     3781 ttaggtattt cagttatgtt ataatagttt tgctgtctgc taccccggcc taatctggca
     3841 aggggaggag gagatataat gaaatacctt tttgattttg tttttgcaat caagccactt
     3901 gcagatgtag tgtgcagtta cctctgcaaa tggcttgatt gcaaaattgc caaaaaagac
     3961 agtaagccta cccgttaatt ctataatata gaaacgtaaa aaaaacccca tcagcctgca
     4021 cgctgatggg gtttggtgat ataatgaaat acctctttgt tactcataat tgtaccataa
     4081 ttagtcacga atgcaaccaa tagaaaaaac tacttagaac ggtagatcat cgctaataac
     4141 aatgcattgt ccaccaaatg gattttgtga agcattaaat acattgttat taattgtcgc
     4201 tgcttttaac taggcctctc cccttctatt ataaaagaag atatatatca tttcttaatt
     4261 acctactacc ttaaaccaaa atttaagtac tacctaattt aagtattact taaacaatct
     4321 aaaaaatagt tatcccaatt gttatgcttt tataatacga ttatttgtaa caaaatgaat
     4381 attatataat taaacactac catatttagg tagtgtttaa ttattagcaa aacaaaaaat
     4441 atttattcta ttctcttaac ttcaactaag ttatgctgca cacctttata taaatcatat
     4501 gcaactcatt agctagacaa actacaagca tgtagtttta tctgttaatt atgtactacc
     4561 taaataaagt agtttataat tagcaagtat atatatttaa aaatttaaga ttaatttttt
     4621 tatgacttgt acaaatttat ctgatattct cttattaaga actacctaat ttgggtagtt
     4681 cttatttttt acattttaag tagtatttaa ttgcaatttg gtagtatttt tttattatct
     4741 tattcttaga acggtagtac ttaatataaa tataggtagt atctaattta agtattaatg
     4801 gtagtatcta aattaacagg tagtatgtaa ctactttaaa gtaatttttt tcagattaac
     4861 acagtagtat cttattaaat aagttaattt taatggtagt acataattat tatatatatc
     4921 tgacattggt agtacttatt gtatttttta gtagtatata atctattgtt ttctagccta
     4981 aagccttgtc ttataagaat aatataatat atactactga aataaggtag tatatattac
     5041 tagtaatggt agtatgtttt atatgcataa ggtagttctt aatttatttt ttgttttgac
     5101 actggtagta cttaattatc tttaatttta aaactaaatt ttattcatac gtttttctgg
     5161 tagtatttaa ttataaataa tatttattta aagtagttct taatgacttt aaaggtagta
     5221 cataattcat tttatttctg attttaggta gtatttaatt aaaaaacagt tttaatgcgt
     5281 ttgcattttt tatttatctt tgctattttc gaaaagagta ggtaatttta cataaataaa
     5341 acaacgtgaa agtagaggat ctacaatgac tgattttaaa ttttttaagg tggatagagt
     5401 ttacaacgaa ttattttatc aatttccaaa agtctttatt gtttctgacg aatacaaaaa
     5461 aatgaaagat tcaactaaga ttgcctatat gcttttaaaa gcaagattag agatcgcaat
     5521 cagcaaacgg caaatcgatg aagaaggtaa tgtttatttt acttatacga caaatgaact
     5581 atgtagagta ttaaactgcc aaaaacaaaa agcgatagca atcaaaaaag agttggaatc
     5641 ctttggttta ttattacaaa agcagatggg atttaacaaa cagttaggga aaaataatcc
     5701 taatagacta tatctagcag aattaaaagt ctcagaaaat gatatctact tactcgaaaa
     5761 atttgataga gagaataggg aaaacgttga taaatcagag ggtatgaaaa tcatacccac
     5821 cctcgacgaa aaatcagacg ctgaatccct tggggctcaa gagggtatga aaatcatacc
     5881 gtgccaaaac gttgataaat cagagggtat gaaaatcata ccagaactta ataataatat
     5941 attagacact aatagacaca atatagacac tgaaaaagac cgcctacaag atcaattgtt
     6001 gttagacaat tttgagacaa ttatgacaaa cgacagcatt gctacgtttg tccctgaacg
     6061 agtattaaat ttgataaaaa cattttcttc aagttacagt gaagctcaaa aaaccgtcca
     6121 gactattcat aatgcaaaga aaaaagctga aatagaaagt ggtatttcga tagtttttga
     6181 agaactcgat agttattatg tcaatgcaga acaagaatta tacacgacac tgttaaaagc
     6241 ctatcaaaaa ttaaaaaccg aaaaagtcga aaatatccag aacctgattt ttgtctatgt
     6301 aaaaaattgg tttatcgaaa aaccaatagc tgctaaagta tcaagtgaaa aacgtttgaa
     6361 ttatgaaagc tccccaagca ctattacgaa agactggtta gagtgaaact ttactgacaa
     6421 agtgattgtt gcttcatgga gtaacattaa aattaaaaat tttttattat tctaattata
     6481 ttacgaatat tacaaaataa tagctatttt aatagaattt ataagttaaa atattatgat
     6541 aaaaatcaca aacgtagcct tttacgtcat aagtgttaaa agaaataatt aatttcaaat
     6601 atagaaaagg gaacgtatgt ttgcatactc cttttttttg agttataata atacgcgaag
     6661 agagataatt tcattattta aacaagctaa aaatcttgat attacttctt ttcaggcaat
     6721 acgaatatta ttcgcttaca ttttttgt
//
LOCUS       DAFELO010000098         6676 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFELO010000098
VERSION     DAFELO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.103"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            71..154
                     /locus_tag="LP452_002603"
     CDS             71..154
                     /locus_tag="LP452_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL2015275.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="LP452_002604"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="LP452_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL2015276.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="LP452_002605"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="LP452_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1609..2274
                     /locus_tag="LP452_002606"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="LP452_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="LP452_002607"
     CDS             2310..2555
                     /locus_tag="LP452_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2015277.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="LP452_002608"
     CDS             2658..3527
                     /locus_tag="LP452_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL2015278.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="LP452_002609"
     CDS             3508..4242
                     /locus_tag="LP452_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2015279.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4275..5190
                     /gene="ant(6)"
                     /locus_tag="LP452_002610"
                     /pseudo
     CDS             4275..5190
                     /gene="ant(6)"
                     /locus_tag="LP452_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5187..5729
                     /gene="sat4"
                     /locus_tag="LP452_002611"
     CDS             5187..5729
                     /gene="sat4"
                     /locus_tag="LP452_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL2015280.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5822..6616
                     /locus_tag="LP452_002612"
                     /note="aph(3')-IIIa"
     CDS             5822..6616
                     /locus_tag="LP452_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL2015281.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2340 a         1005 c         1393 g         1938 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat aacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac attcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcactttttg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag
     4321 aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac
     4381 ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata gaacctagaa
     4441 ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag
     4501 ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt
     4561 gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta
     4621 aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata
     4681 gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc
     4741 tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt
     4801 ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca
     4861 tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt
     4921 gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc
     4981 tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt
     5041 gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat
     5101 accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc
     5161 gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag
     5221 atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     5281 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     5341 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     5401 agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa
     5461 attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc agggggcaag
     5521 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     5581 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg
     5641 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     5701 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     5761 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
     5821 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
     5881 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
     5941 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     6001 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     6061 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     6121 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     6181 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgatt
     6241 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
     6301 agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
     6361 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
     6421 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
     6481 cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct
     6541 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
     6601 ggatgaattg ttttagtacc tagatttaga tgtctaaaaa gctttaacta caagcttttt
     6661 agacatctaa tctttt
//
LOCUS       DAFELO010000099         6644 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFELO010000099
VERSION     DAFELO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.104"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(1..859)
                     /locus_tag="LP452_002613"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LP452_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LP452_002614"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LP452_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL2015282.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LP452_002615"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LP452_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LP452_002616"
     CDS             complement(2143..2373)
                     /locus_tag="LP452_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBL2015283.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LP452_002617"
     CDS             complement(2603..3421)
                     /locus_tag="LP452_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2015284.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LP452_002618"
     CDS             3582..4271
                     /locus_tag="LP452_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL2015285.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LP452_002619"
     CDS             4285..5787
                     /locus_tag="LP452_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2015286.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LP452_002620"
     CDS             5800..6276
                     /locus_tag="LP452_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2015287.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LP452_002621"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LP452_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFELO010000100         6508 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFELO010000100
VERSION     DAFELO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.105"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            599..1288
                     /locus_tag="LP452_002622"
     CDS             599..1288
                     /locus_tag="LP452_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2015288.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1275..2483
                     /locus_tag="LP452_002623"
     CDS             1275..2483
                     /locus_tag="LP452_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2015289.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2777..4048
                     /gene="serS"
                     /locus_tag="LP452_002624"
     CDS             2777..4048
                     /gene="serS"
                     /locus_tag="LP452_002624"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL2015290.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4597..6081)
                     /gene="guaB"
                     /locus_tag="LP452_002625"
     CDS             complement(4597..6081)
                     /gene="guaB"
                     /locus_tag="LP452_002625"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL2015291.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1983 a         1220 c         1253 g         2052 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctcttttttt aaaagttttt tcgatttcgt ttttcgaaat
      121 cttccagcaa ctcatttatc ataacttatc gtttgttatt tgtcaagaac ttttttgaag
      181 tttttttcga tagaatcaag cttcgcaagt ggttcttttt taacgactca gttatcttat
      241 catgtcattt ccaacatgtc aacaacttat tttttattat ttaataagtt attggtgttc
      301 ttttgaacaa ataataatat atcaacatca catgtgaaag tcaataagaa aatgcttatt
      361 tatcaacatt tctagcttac atgatcattg gttagttctg gtcctttttc gttcgtcttt
      421 ataactccga gagacgtaaa atccttttgt tttcagttta atgttcatgt tcttgtctta
      481 ttcacaccaa ctagatgtct caccttctaa aattttcctt aacaaagtgc tgcttttatc
      541 tatttctgct tttttttgct agaataatag taacaatatg actctggagg atttatttat
      601 gaaaattttg attgctgacg atgataaaga aatcgttgaa ttattaagta tctatgttca
      661 caatgagggg tatgaagcag taaaggctta tgatgggaaa gaagcattat ctaaaatcag
      721 aacaatccct gatattgaat tacttatttt agatatcatg atgcctgaga tggatgggat
      781 gcaagtagtg aaagagctac gaaaagaatc ccaaatacca atcattatgc tgacagcaaa
      841 aactactgat atggataaga tcaaaggact ggtcgctggt gcagatgatt atgtgaccaa
      901 gccatttaat ccgttagaag taatggctag agtaaagtct ttattacggc gtacaaaaat
      961 gcagattgcc ccagatcagc ctgatgtatt agaagtaaat gcattaatca tcaataaaga
     1021 ctctcatgaa gttaaaacaa tcgatgggaa agaaatccag ttaacagctt tggaattcgg
     1081 tatcctctac ttgcttgcta gtcatcctaa tcgagtattt agcgcggatg aaatttttga
     1141 gcgtgtttgg aaacaagaaa gtattgtctc tgctaaaact gtcatggttc acgttagtca
     1201 tttaagagat aagattgaag aagcaactgg tggtgaaaaa gtcattcaaa ccgtttgggg
     1261 agtgggctat aaaattgacg caagatagta aacgagcaac tgaaaaacgc cgtcggatca
     1321 atttgacttc aaaagaaatc agcgaattgt tagcggaagg aatcatcacg attatccttt
     1381 tgcttctatt aaatgtttct attctggttg ttatcagttc ggtgatcaac agttcacctt
     1441 ctttgacgaa tgcgatttgg gattcaaaga atatttttgc agaacgattg aacactgatt
     1501 tgttctggaa cggccggaat tttattattc cctttttctt tctattagat attggtgttt
     1561 tgtattggcg cttgattcga agatatcgcc aaatgcaatt gcggcatatc atcagcgaac
     1621 ttcactacat cgcaaacgga aattatgatc atcgtatccc ttttgaactg agtggtgact
     1681 taagccgagt ggtaacaagt atcaatggat tggtggatag taccgtagca gcgattgaag
     1741 acgaacgtaa aatcgaaaaa tcaaaagatg aattgatcac gaatgtcagt catgatatcc
     1801 gtaccccact cacgtccatc attggatacc taggactgat agaagatggc cagtatcact
     1861 cagaagaaga tcttttaaag tatacgcata ctgcttatat caaagcaaag cagatgaaat
     1921 ctttagtgga cgacttattt gaatacacta aggtacgcca gcctgctgtc ccggtgaatt
     1981 tttctgcctt tgacatgatc cagttgatcg aacagttagc agctgatttt gagttggaag
     2041 cttctaaaaa aaatatccaa atacttgttc aatcaaaagt agactccctt atcatggatg
     2101 gcgataccga aaaacttgta cgtgtcttca ataatctttt gacgaatgcg ttgaaatacg
     2161 gaaaaggagc aacgaagatc gtaatcgaag tagaacgtat cggttcagaa gttgttgcaa
     2221 cagtcaaaaa taacggagca atgatcccac aacaggcaat cgataatcta ttcgaccgtt
     2281 tttatcgtgt agaagaatct cgttcacaag caactggagg aactggactt ggtttagcaa
     2341 tcgctcaaag cattgtggct ttgcacggcg gatacattta tgcaaaatca gataagcaat
     2401 ggacttcttt catcatgcac cttccaatca agaaaaatga aaaattacct attgatacac
     2461 aggaagttat tgacgaaagc tgaaatattc gctattctta ttacaattat tagaataaat
     2521 gaaaaacaca gaacccagta cactttattc tttttcagag agctgatggc aggtgtaaat
     2581 cagcaaagga taatttggaa gttccagttc gtttacgtgc cctttcgaaa taagaaaggt
     2641 cggttcaaca gccgttatgt gttcaacaag tgcagctgac gctgaacttg ggtggtaccg
     2701 cgaatttcag actgattcgt cccaagaata atgggatgga tcagtctttt tttattttca
     2761 aaaaggagga aatcatatgt tagatgtaaa aatgatgcga caaaatattg aagaagtaca
     2821 agaaaaattg atgactcggg gcgtaaaaaa agaaacatta gctgaattca tggaactaga
     2881 tgaaaatcgt cgtaagctac tagtcaaaag tgaagaatta aaaaaatatc gaaatgatgt
     2941 ttctgctgag atcgcccaat tgaagcgtgc aaaagaagat gcttctgcta aaatcgctga
     3001 aatgaaagaa gtcggtggca acatcaaagc attggatact gaaattgctg aagtggatga
     3061 aaaattgaaa caaattgcaa ctactttgcc aaaccttccc catgattctg tgccagttgg
     3121 aacagatgaa gaggacaatg tagaaatccg tcgttggagc gaaccacgta catttgcttt
     3181 tgaaccaaaa ccacactggg aaattgcaga aaatcttgat attttagatt ttgaacgtgg
     3241 cgcaaaagta gccggtagcc gttttgtcta ttacaaagga ttaggtgctc gattagaacg
     3301 tgctgtctac aacttcatgc ttgatcagca tatttacgaa cacggctata ctgaaatgat
     3361 cactccttat atcgtcaaca gtaaagcgat gtttggtacg ggacaatttc caaaattcaa
     3421 agaagatgtt ttccaattgg agaatactga tctaacatta atcccaacag ctgaagtacc
     3481 attaactaac tattataatg atgaaatttt agatggtgca gatttaccta tttatttcac
     3541 tgcactaagc cctgcttttc gttctgaagc cggaagtgct ggtcgggata cacgaggact
     3601 gattcgtctt caccaattca ataaagtaga aatggtcaaa ttttcggatg tagagcattc
     3661 ttacgaagaa ttggaaaaaa tgacagctga tgcagaagat atcttgaaaa aattgcattt
     3721 gccttatcgt gtgatggcct tatcaactgg agacatgggc ttttctgcag ctaaaactta
     3781 tgacttagaa gtatggatac ctgcacaaga tacttatcgt gaaatcagtt cttgttctaa
     3841 ctgtgaagat ttccaagcac gccgcgcaat gatccgttat cgcgataatg atggaaaaat
     3901 ccactatact catactctga atggttcagg acttgcagtt ggccgtacag tagctgccat
     3961 tttggaaaac taccaaaatg aagatggttc tgtcacagtt ccagaagcgc tcgttcctta
     4021 tatggggaat ctgaaagtta ttaaataaag aggctgtgaa aaagccgctt agtttcgagt
     4081 attaagaaaa attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc
     4141 gagaaactgg cttttgaaca ccatttatcg agaggttgtg aaatcagcga attaacctga
     4201 gtattagaga aaaaaatgga aaaatagtcc ctcatatttt accgtttgtt gaactaatac
     4261 cgagggtcag ctgattgaac accgtttagt ggagaggtcg taagcctacc ccccagctcc
     4321 tagttacaag gacaaattca aaaaacagct cttcatattt tatgaatgtt gtacttgtga
     4381 ttcggaggtg gcaagggaac actgtttatt caaaaagagg ctgggacaga agcgtttaac
     4441 tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcttctt
     4501 atttccgaag gagttgcttc tacttccgcc gtttatacgt gtttagagcg tgagacaaaa
     4561 gtgttcttta cttttgtccc acgctcttta atgtttttaa gattcaactg aatagtttgg
     4621 tgcttctttt gtgatttgta catcatgagg gtgtgattct tttaatccac tgccgctcat
     4681 ttggataaat tgcgcttcat cacgtaattg ctgtagattg gctgccccaa cataacccat
     4741 tcctgaacgc aaaccgccga tcatttggaa aataatatct gcaacacttc ctttataagc
     4801 aacccgacct tcgattcctt ctggtactaa tttatttgct tcattgacac taccttggaa
     4861 gtaacggtct tttgaacctt tttccatcgc gcctaatgag cccatacctc ggtatgtttt
     4921 gaatcgacgt ccttggaaga tttcgaattc acctggtgat tcatctgtcc ctgccaacat
     4981 acttcctagc atcactgcat gtccgccggc agctaatgct ttgacgatgt caccagaata
     5041 tttgatccct ccgtctgcaa taatcgcttt gccatattga cgagcaactg atgctgcatc
     5101 ataaatagct gtgagctgag gtacacctac accggctacc acacgtgtcg tacaaatcga
     5161 tcctggtccg ataccaactt ttacgacatc tacacctgca tcatataaag cttttgtcgc
     5221 ttcagccgtc gcaacgtttc ctgcaatcaa tgttgcatct gcaaaagttg aacggatttc
     5281 ttgaattttt cggatcacac ctgcactatg tccatgagct gtatcgatga tgattgcatc
     5341 tgcccctgct tctaataaag cattcgcacg ctcaaatgta tcgcttgtta caccaacggc
     5401 tgctgctaca agcaatcgac catgttcatc ttttgctgca tttggaaatt cgatcacttt
     5461 ctcgatgtct ttgatcgtga tcaatccgct taaacgacct tcgttatcta cgattggtaa
     5521 tttttcgatt ttatgttttt gcaaaatctt ttctgcatct tttagagagg tacctactgg
     5581 ggcagtcact aagttgtctt ttgtcatcac atcagcaatc ggcatgtgat aatctgttac
     5641 aaaacgcatg tcgcggtttg taattatccc taccaatttt ctgttttcaa gtgtttcaac
     5701 gattggcaca ccactgatac gatatttgct catcaattct tctgcgtctg ctacaagatg
     5761 ctgtggcgtt aagaagaacg gatcaatgat tacgccactt tcagaacgtt tgacttttct
     5821 tacttcatcc gcttgctggc tgatcgtcat gtttttatgg atcacaccta gtccgccttg
     5881 tcttgccata gcaatggcca tcttgctgtc ggtaacagta tccatacttg cactgatgat
     5941 tggaatattc aacttaatat ttttagctaa ttgtacgctc atatccactt cgtttggtaa
     6001 cacgtgactt tctgctggta tcaataagac atcatcaaaa gtaaatcctt ttttcgtgaa
     6061 ctttgtttcc cagttggaca tttttagtac cactcctcta atttagttta tctattaaaa
     6121 taattgattt tttcatttaa gtcaatgatc tacttggaat caagttagct ttctcataga
     6181 ataccaatat aaagcgttta catctgtgta tattaaaaga gattgtaaaa aagctgtcct
     6241 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga attggttggg
     6301 tttattaccg cagatgcaaa cttttgaaca ccgtttagtg gagaggctgt gaaaaagctg
     6361 cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
     6421 tggacttaat accgagaaac tggcttttga acaccgttta tcgtaaaaag ggactgtgac
     6481 aagacttttg tcacagtccc ttactatt
//
LOCUS       DAFELO010000101         6467 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFELO010000101
VERSION     DAFELO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.106"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(110..565)
                     /locus_tag="LP452_002626"
     CDS             complement(110..565)
                     /locus_tag="LP452_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015292.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="LP452_002627"
     CDS             complement(719..1927)
                     /locus_tag="LP452_002627"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL2015293.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="LP452_002628"
     CDS             complement(2039..2371)
                     /locus_tag="LP452_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL2015294.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="LP452_002629"
     CDS             2519..3394
                     /locus_tag="LP452_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2015295.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="LP452_002630"
     CDS             complement(3476..4057)
                     /locus_tag="LP452_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL2015296.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="LP452_002631"
     CDS             complement(4063..5322)
                     /locus_tag="LP452_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL2015297.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="LP452_002632"
     CDS             complement(5324..6370)
                     /locus_tag="LP452_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015298.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1803 a         1338 c         1102 g         2224 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcact tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaa
//
LOCUS       DAFELO010000102         6379 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFELO010000102
VERSION     DAFELO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.107"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(98..1228)
                     /locus_tag="LP452_002633"
     CDS             complement(98..1228)
                     /locus_tag="LP452_002633"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL2015299.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3078)
                     /locus_tag="LP452_002634"
                     /pseudo
     CDS             complement(1877..3078)
                     /locus_tag="LP452_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3138..3341)
                     /locus_tag="LP452_002635"
     CDS             complement(3138..3341)
                     /locus_tag="LP452_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL2015300.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3483..4715)
                     /locus_tag="LP452_002636"
                     /pseudo
     CDS             complement(3483..4715)
                     /locus_tag="LP452_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4878..5690
                     /locus_tag="LP452_002637"
     CDS             4878..5690
                     /locus_tag="LP452_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2015301.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1910 a         1306 c         1006 g         2157 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 acactgtctg agttttaagg aaagcttctt ccccaaccca atcgttcgat aagcacattt
     2281 agttttggga gtcgaaaagt accaatcatc ttttgtatta taaatcaatg tcttctcaat
     2341 cgttatcgtg tttttctcaa aatctacatt cgaccaagac aaagcttcaa gttctccaat
     2401 ccgaatgcca gtataaaaga gcatttcata cacaatcttc cgatggtggg tttgaatctt
     2461 atcatccttc aattcattca tcactttttg aaactcactg tgttcccaaa actcaacttt
     2521 cttattctca gaaggaagcg aagaaacatc ttcaacggga tttttagcaa tcaccccctc
     2581 tttaacagcc cgatccaata caatttttag gtgccctcga atccgattga tataagattt
     2641 tgataacgtt tcgccattaa cttttttccg tgaattacgt gactctgtct ggcttagttc
     2701 aaataaccaa tcctcaatat cgtgacttgt aatatctcga acacgaaaat gatagaaaaa
     2761 ctgcatacgc ttttcgaaaa tgaattgcgc attctcatat gtttgacttt tgacctgtct
     2821 ctcgtaccat tttacaaacc tatccttgta aaactcttcg aaagtcattt gattggtaga
     2881 acacagttga ttaggaattg tcttgttaaa atcatgtgtt tccatgtact tgtcataagc
     2941 aattttggca tctttttgtg aagcataacc tcgtttagtc ttctgaacct ttcttccatt
     3001 ttcatctata cctagatacg ctcggaagaa ccattttttt gtctttttat cttggtaaat
     3061 atttttaatt tttggcatcg ttccattcct ccctgtactt gagaaaagct actttaatag
     3121 taccgcgctt gtactgttta ttccgcaact tgaatgtgaa aaagctgttc aattactcgg
     3181 gatggcacca caccaacttg gcgattatta taaaaatcaa taccttcaat ccttgcaaga
     3241 tatgccttgc tctctttaat cattcgtgca gcttgactag gtttaaagcc catctctatc
     3301 aaaattgcac gatttaccat gaaatcacat tttgtttcca ttctctcacc tcctgattct
     3361 ttactccttt taaaataatc ctctttataa tctattgttt ctcattcgat ggttgtgtta
     3421 tactcgtctt gtcgaggcgg agtacgttgc acaacttgtt gtgtaagcag tactttgctt
     3481 ttttagtagc ttgttttctc atttgatata ctttgtaatt ttcgttcata atactcaacc
     3541 tcctcctgat aatactccgg gtctgccttc atattttgga gaagtttgat ctgtcttgga
     3601 ttcatttctc cttgttcaat aatcaaaggc aacaaggaag aatgaaacgt ttgatcgacc
     3661 tgtgcaataa aaactaatga tggtgaaaca ccatgaatta accaactcat agttttctca
     3721 aaactttgag gaattgtatc taaagaaatg gtgattgcgc cataattgct aataaaggct
     3781 gcccatttgg catcaacttt tgcacctaga tcatttggat cacggtcata aaaacaaatt
     3841 gctccattaa gtaactcaaa gaacagtttg gttaaatcat gggtttttga aataatcttt
     3901 gccaagcttt gcgctttctc ttttcgatac cgtagttcaa agcgattctt tacttttgcc
     3961 tcttctaaag gaattcctcg tcgcttccgt tgttcatggt ccttctgata aaagcaaaaa
     4021 cgacactgac tcttctttga gcctaaatac aaggtctcac catttgattc attgtttatg
     4081 atatccgtac cgccatgaga ttcactcact tgaaaagtgg tccacacatg gcctagtatt
     4141 accttatgct taagttctgg aatgctgaga ctccccacaa aatcatctaa agtaaaatca
     4201 atccgtgtaa agttcccctg ataatctaat accttacgaa aaaactgttg ccaatttgtt
     4261 tttcgtgttt ttagccatct tgctaagtgt ttacagcctt gacctgaaaa ctccatcaca
     4321 gttcctttaa ccgcatcgtc aatggaatac cgaatcgtga ttacttgatt ccacacgtat
     4381 tggccaatat agccctttgg cgcataatcc agttcctcaa aacgttcaat gggcatttca
     4441 ataatctctt caattagctt taaatgttga ttcgagaaaa agtgaatggt tagataatca
     4501 atcaaaacac ggttaactgg aacgacctct tcttgcaaac tatccaacac aagattcaat
     4561 ttttcttcac ttaattgtct cgttcctttt tctactttcg ataataacta tctcgaaatc
     4621 cccacttttt ctgctaaata tcgttgtgtc cacccccttt cttctcgaga aactcgaatg
     4681 gcttcaccga gatcatgttg attaacttgg ttcatcgaca aacctccttc tgctatttac
     4741 ttgtcaaaga acctattgaa cttattttaa gtacattttt attgtacgcc tgtatttatt
     4801 ttaagtcaat atgttttgac tttttttaag tacacacgta tactaaattt aagaacttat
     4861 cctgaggagg agttttcatg gagatagaca agcaagcagt cggcaatcga attcggcaga
     4921 ttcgccaaga attaaagttg agtatggaga aatttggcaa actaattggt gatttaccgc
     4981 gaagtacagt caataactgg gaacgtggga tcaaccttcc aaaaaccgaa acccttcacc
     5041 aaatcgcaga gataggtcat gtcaccaatg aatatctttt gtatggtgat caagagaatc
     5101 aatacatttt agaaatgctc caaaaaaagg caggtaagct tgatcctaag attgaaggtc
     5161 tcatagtaga cgaaatcaaa cacgctgacc ttgttagtga aaaagaaatg gatcgaatga
     5221 ttgaattttt tattacaaat ctcattccac caactgaaca agaccagttt actttccaat
     5281 gtatcgatga aaaaaagcag ctttatcttg gttcaactaa ttttggtaag gaagcccagc
     5341 tctatttgca acatgataat caaaatcata ttctgcacat gatgcccttt accttctcca
     5401 actttagtgt ggatcgcata ctcgtctatt tagcaaatca aaaatcctat tcctattttg
     5461 ggaaacattt acccaaggaa ctaggtgaga aagccatcct tctatactca attaatcaat
     5521 tgaccagcga tgtgcgaatt gccccgttgg tctattcaga agaaaccgct tcttatcagt
     5581 acacagagga caatcaatat ctactagaac agcaatatct ttatctacca ttcgtgatgg
     5641 aagtcgagaa agaacgcctg ttaaatgcag cgtatccatc atcaaaatga ggtgacaaaa
     5701 ttgataagca cgtgacaaag aaaatactgc taggacactc cccaatgggt agttgtcaca
     5761 gtttcctgtt ctttgttagc cccctgttag aaccgggggc tttattcctg cagtccggct
     5821 acgccttcct gcaatagaca aaactccaga tcgggtaatt cgcttgtggc gaaacccgac
     5881 ctggagtttg tctctgtatt ttgaacccta aaaattaatc aaacagactg atattctcat
     5941 aatcagcgcg aaataagcca ttaagtacag ttttcctacc tatttttgat aacccgctgg
     6001 ttagttaata ttccttttcc gagagagata aatgattggt taaatctata tacatctcaa
     6061 cctttaaaaa tgttttcgaa atacttcatc agtacatgtc atacaaatta ccagtgcatt
     6121 ctcaaattct agttctttaa ttgatttcag ttgaggagca gttacttttg gcggaaattt
     6181 ccatttttgt ccaaacctct tcccccgatg aaatttcttt gttgcaatta ttgcaatgaa
     6241 aaatttttgt ttcaaaaata tacataatta cttcctccaa taaaaactat ctacctctca
     6301 aattattttt ctctaataag aaaaacagaa aaggtaataa gatacaacag tgatgattcc
     6361 taataaagaa aagactaca
//
LOCUS       DAFELO010000103         6265 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFELO010000103
VERSION     DAFELO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.108"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(95..667)
                     /locus_tag="LP452_002638"
     CDS             complement(95..667)
                     /locus_tag="LP452_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2015302.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LP452_002639"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LP452_002639"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL2015303.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="LP452_002640"
     CDS             complement(1352..1867)
                     /locus_tag="LP452_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBL2015304.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="LP452_002641"
     CDS             complement(1869..2153)
                     /locus_tag="LP452_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2015305.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="LP452_002642"
     CDS             complement(2187..3515)
                     /locus_tag="LP452_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2015306.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="LP452_002643"
     CDS             complement(3529..3984)
                     /locus_tag="LP452_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2015307.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5933)
                     /locus_tag="LP452_002644"
     CDS             complement(3987..5933)
                     /locus_tag="LP452_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2015308.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2060 a         1205 c          875 g         2125 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taattttaat aaatttacat gtctttcaat caaacttgtt
     5941 cacctcttaa cgctagcatt tgttacctct tttcattatt tattctagca ctggaacccc
     6001 cttccctcaa ttacatctat gtcaataata cttgtggaag tttgttgtaa aggaaatcca
     6061 aataatctta aaaaatgaga aacaaaaaag gagaaatcct taaaagagct aagatattcc
     6121 aatagaaaat actccttatt atggaagtaa tatgaaatat tacaattgaa aaataaaaac
     6181 aatataatgg cgtcactaaa attcgtattt ttttattata atataactta attacaaatg
     6241 ctcatctatg aacaattgag tccta
//
LOCUS       DAFELO010000104         6173 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFELO010000104
VERSION     DAFELO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.109"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            725..811
                     /locus_tag="LP452_002645"
     CDS             725..811
                     /locus_tag="LP452_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL2015309.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="LP452_002646"
     CDS             complement(932..1621)
                     /locus_tag="LP452_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015310.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="LP452_002647"
     CDS             complement(1628..1906)
                     /locus_tag="LP452_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015311.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="LP452_002648"
     CDS             complement(1866..2780)
                     /locus_tag="LP452_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL2015312.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="LP452_002649"
     CDS             complement(2762..3142)
                     /locus_tag="LP452_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL2015313.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="LP452_002650"
     CDS             complement(3592..3879)
                     /locus_tag="LP452_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2015314.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="LP452_002651"
     CDS             complement(3899..4123)
                     /locus_tag="LP452_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBL2015315.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="LP452_002652"
     CDS             complement(4578..4748)
                     /locus_tag="LP452_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015316.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="LP452_002653"
     CDS             complement(4763..5296)
                     /locus_tag="LP452_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL2015317.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="LP452_002654"
     CDS             complement(5317..6159)
                     /locus_tag="LP452_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL2015318.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAFELO010000105         6135 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFELO010000105
VERSION     DAFELO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.110"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(207..1169)
                     /locus_tag="LP452_002655"
     CDS             complement(207..1169)
                     /locus_tag="LP452_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2015319.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LP452_002656"
     CDS             complement(1171..2610)
                     /locus_tag="LP452_002656"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL2015320.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LP452_002657"
     CDS             2795..4741
                     /locus_tag="LP452_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL2015321.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="LP452_002658"
     CDS             5005..5877
                     /locus_tag="LP452_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2015322.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1947 a         1054 c         1084 g         2050 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt ggggggcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttctt
//
LOCUS       DAFELO010000106         6043 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFELO010000106
VERSION     DAFELO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.111"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(108..977)
                     /locus_tag="LP452_002659"
     CDS             complement(108..977)
                     /locus_tag="LP452_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL2015323.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(1269..2879)
                     /locus_tag="LP452_002660"
     CDS             complement(1269..2879)
                     /locus_tag="LP452_002660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL2015324.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4072..5412)
                     /gene="glnA"
                     /locus_tag="LP452_002661"
     CDS             complement(4072..5412)
                     /gene="glnA"
                     /locus_tag="LP452_002661"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL2015325.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(5440..5832)
                     /locus_tag="LP452_002662"
     CDS             complement(5440..5832)
                     /locus_tag="LP452_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2015326.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1782 a         1286 c          996 g         1979 t
ORIGIN      
        1 ataaaaaaaa gaaacgagca tggtctgccg ttttaagtaa aaaagaatgg agagcgactt
       61 caaaagtcgt tctccattcc tatatatcgt atctgttatg tggatgctta cgctttgtta
      121 gctgaaccga accagtcgat atgagattta gcatcttcaa cgattgctgc tttacctgga
      181 gctaataatt tacgagggtc gaatccttta ccttctttat ctttaccagc ttcgatatat
      241 ttacgagtag ctgcagcaaa tgataattgg aattcagtgt taacatttac ttttgagata
      301 cccattgaga tagctttttc gatttgatct tgaggaatac ctgatccgcc atgtaatact
      361 aaaggcatgt ctggacctac agcatcagca attgcttgta agtggtcaaa agctaatcct
      421 ttccagtttt ctggatattg accatggatg ttaccgatac cgcaagctaa gtagtcgata
      481 cctgtagcaa ccatttgttt gcattcttca atatcagcta attcgccgtt accgatgatt
      541 ccgtcttctt ctccgccgat tgaaccaact tcacattcaa ctgagatacc ttttgcatgt
      601 gctttttcaa caacgtcttt agctaatttc aagttttctt caaatggtag atgtgaacca
      661 tcgaacatta ctgaagtata gcctaattcg atacattcca aagcagcttc gtaatcacca
      721 tggtctaagt ggatagcaac tggaacagtg atgttcattg cttcaactaa atcagtgatt
      781 aaatctttac agattttgta accacccatg tatttagctg cacccattga tgtttggata
      841 agtactggtg cttgttttgc ttcagccgct tcaaggattg cttgagtcca ttccaagttg
      901 tttgtgttga atccgccgac agcgtaaccg cctttacgag cagcttttaa aaattcagct
      961 cctgatacta ctggcataat aaaattcctc ctaagttttg taaaactaaa tttgttaaga
     1021 caattcttaa ccaacgaccc tattttacca aacatttcgg tagttttcta ctaaaatcaa
     1081 tgaaaatgaa aattttcaag aagttttttg caaaaaataa aatatccaat agcgctttaa
     1141 tcgaaaaaag tcttttgctt tctatctttt ttcctacaaa taataacaaa tcagaaaaag
     1201 agaaaagtga caagaaaatt gcttatcccc ttctcttgct acttttctct ttctatcaag
     1261 ttcaaaaatt attttgtttc gtgatctgct aatgatgctc cgacaaatgc tttgattaat
     1321 ttttgcgggc gatttggacg agaaatcaat tctgggtgga attggcatcc cacaaagaat
     1381 tttttctctg gcagctctac gatttctact aaacggttat ctggtgagac acctgaaaag
     1441 actagccctt gttcttcaaa caattgacga tatttgttat tgaactcata gcggtgacgg
     1501 tgacgttctt gcacaacatc ggctccatca tatgcttcag ccgtcttcgt tccttttttg
     1561 attttacatg ggtataatcc aaggcgtaaa gttccaccca aattctcgat gttttcctga
     1621 tcagccatta aatcaataat attgtttgct gtatctggat tcgtttcagc tgatgacgca
     1681 tcttctaatc caacgacgtt acgtgcaaat tcgacacaag ccatctgcat accaagacaa
     1741 atacctaaaa acggaacgtc attttcgcga gcataacgaa tcgcttcgat tttcccttct
     1801 actccgcgat caccaaatcc accaggaacc aagattccat ctgcgtcttt caaacgttct
     1861 tctacatttt ctctcgttaa ttcttgtgct ttgatccaat cgatctcgat gtctgaatta
     1921 taggcgaaac cagaatgttt caatgcttca actactgaga tatatgcatc tggtaactcg
     1981 acatatttac cgacaagcgc aatccgtact tttttcttca agttcaagac acgttcttct
     2041 aattctttcc attctgtcat gtctgcttct ggtgtatcta gttttaaatg gtcgcaaaca
     2101 atttgatcca ttccttgtgc ttgtaaaact aatgggatgg agtaaagtgt atctacatct
     2161 ggcgactcga taactgcttc tggttcgaca tcacaaaatt gtgccaattt gtttttcacg
     2221 ctttgagaaa caggttgctc tgtacgtacc accaaaatat tcggttggat acctaaacca
     2281 cgtagctctt ttacactgtg ctgcgttggt ttagttttca tttctccagc agctttcaag
     2341 taaggaatca atgttgtatg gatatacata acattatctg taccgacatc tgctttcatt
     2401 tgacgaagtg cttcaaggaa tggcaaagat tctatatccc ctactgttcc ccctacttct
     2461 gtaatgatca catctgaatc agtcatttta gctgctcgca tgattttttc tttgatctca
     2521 tttgtaatat gaggaataac ttgaacagtt gctcccaagt attcgccctt acgttcttta
     2581 cgcaaaactt cagaatagat ttttcctgtc gtcacgttgg agtatttgtt caaattgatg
     2641 tcaataaaac gttcgtagtg gcccagatcc aaatctgttt cagcaccatc atctgttacg
     2701 aatacttctc cgtgctgata tgggctcatc gtccccggat ccacgttgat gtatggatca
     2761 aatttttgga tagttacttt taagccgcgg tttttcaata agcgtcctaa agaagctgca
     2821 acaatccctt ttccgattga tgaaacgaca ccgccggtca caaaaatata ttttgtcata
     2881 agttcaaaaa ctccttttta atttatgtgc aggaaaagtc tcagacaata aacaaagctc
     2941 cctattcttc aataagaata gggagccgaa atttctgtaa acttctgacc ctctaaaagg
     3001 gtgcccaagg tgtataatac aagctgacta agggaaagtc aagtactcaa atgaatacta
     3061 tccgcttaca attttttctt tttcgatatc ctttgagaaa ttatttctta agtcctttat
     3121 gagtgcaaaa aaaatttaac ccacataata agcttgatat aaacatagct aacgttaaac
     3181 ctaagcaaat cagaaaatcg ttactgaaaa attttaagtg aaataataga taaaaaaata
     3241 aaacaaacat tatgatagat aatatcccac ctaacaaaga actacttttt cctacttgca
     3301 gcaacagttt atatagctta tcgtagaaac aatctttttc tttcaccctt atttacctcc
     3361 tttacgtaaa aaacgtagtc cacagttctc ggatttttgg catatctttt ctattaaata
     3421 atatcaaaaa caaaacaatt ccactaattt tttcaaagat gacctttttt tcaacaaaag
     3481 tgccttgtag ggattcattg aaggtaacga atcacataag taaaaaataa ggagatgcga
     3541 caaaagtcgt gcctccttat ttctaaataa acggtgttct cctgccaact cctcggtcac
     3601 aagtcacaat atccatcaaa catgagaagc tgtttttgaa attatttctt gtgattcgga
     3661 gttaaatggt aggctcacaa cctctggata aacggtattc aaaagccagt ttctcggtat
     3721 taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttaatactcg
     3781 aaactaaacg tctttttcaa aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg
     3841 taataagttc agccaattca aaaacaagaa aagctgtttt tgcttggcta ttcttattac
     3901 ttgttgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct
     3961 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt tttccgttag tttctctaat
     4021 actcaggtca aaacgctgat ttcacagcct ctaattcacg gtattcctgt tttaataaag
     4081 ttctagatat tgttctcttt cccactcgga aacagtttgt ctgaatgccg cccattcgag
     4141 acgttttgct tcaacaaaat ttgagaagat atgttgtcct aatgcgtcaa tcattacatt
     4201 atccttacgc aattctttga tagcattatg gatagttgaa ggtaagtcct ggatatgtgc
     4261 agcttctctt tcttcctcat ccattacata aatgttacgg tcaacagctg ctggcggtgt
     4321 gatctgatta cggataccgt ctagtcctgc ttgcaacaat actgccattg ccaagtatgg
     4381 gtttgcagat ggatcaactg accgcaattc caaacgtgtg gataaaccgc gagattctgg
     4441 tacacgtact aatggcgaac gattacggcc actccatgcg acataaacag gcgcttcata
     4501 acctggaacg agacgtttat aagaattgac tgttggattg caaacggctg tgtaggcacg
     4561 agcatgtttc agcaaaccac ctaagaaatg ataagctgtt tcacttagtt ccaattcacc
     4621 gtctttatca tagaaaacat ttccttcatc attgaacaaa gacatattgc aatgcatacc
     4681 agaaccattg atcccaaata acggtttcgg catgaaagtc gcgtgaagtc catgcttgcg
     4741 cgcaatcgtt ttgacaacga gcttgaaggt ttggatatta tcacaagcat ctaccacatc
     4801 tgcatattta aagtcaattt catgctgtcc tggcgctact tcatgatgtg aagcttccac
     4861 ttcaaagcct agactttcta attccaacac gatgtcacgg cggcaatttt cacctaaatc
     4921 tgttggtgca aaatcaaaat aaccaccttt atcattcaag gtcgttgtga tcccaccgtc
     4981 ttcgtctaat ttaaataaga agaattctgg ttcaggtcct aaattgaaag aggtaaaacc
     5041 aaggtcgcgc atatctttca atgctcgttt caaattcccg cgaggatcgc cggcaaatgg
     5101 tgtcccatcc ggattataga tatcacaaat taaacgagca acttttccat gagcactttc
     5161 ccacgggaag atcatccaag tggagagatc tggatacaaa tacatatcgc tttcttcaat
     5221 tcgaacaaat ccttcaatcg aagaaccgtc aaacatcatt ttattttcta atactttgtc
     5281 gatctggctc actggaactt ctacattttt gatcgtacct aagatgtctg tgaacatcaa
     5341 tcgtaaaaat cgtacgtttt cttcctcaat gattcgtttg atttcttcac ctgttatctg
     5401 tttctttacc atggtatccc tcttcctttt ttaatttatt cagtcaaaga attgtctttg
     5461 caatccttgc gactgataat gacctgtttt gcttaaccca ccttgcgcca aaatctcatc
     5521 acgaaaaatt tgtcgaacgt catgatctgt caacggcttc ttgttttttg cttcttgttt
     5581 tttcgcctgt gccatttcgt atacacgttt gatcccagcc atattcaatc cctctgaaag
     5641 atagtcttta atctcaagca acatatcaat atcattcagc gaatacatac gtcggtttcc
     5701 ttcgcttcgt tccggatgaa ctaaatcttg ttcttcataa tagcgaattt gccgagcaga
     5761 caaatcagtc agcttcataa ctgtaccaat tggaaatacg gacatagatc ggcgcaattc
     5821 cttttctccc atcagacgtc ccctttcgtt ttataagttg gctaaagtgt accaattaaa
     5881 aaaaatattg tcaactgcat atgttaggtt ttctaacatg agatctctat tttttataaa
     5941 ataacaaaaa aataagaaca caaaaaaggt atctagttga ttttcgtacc agataccttt
     6001 tattttttat tatactgtac tatcctttat tagtgttttt att
//
LOCUS       DAFELO010000107         5975 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFELO010000107
VERSION     DAFELO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.112"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(313..2205)
                     /locus_tag="LP452_002663"
     CDS             complement(313..2205)
                     /locus_tag="LP452_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015327.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="LP452_002664"
     CDS             complement(2202..3929)
                     /locus_tag="LP452_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015328.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="LP452_002665"
     CDS             complement(3945..4595)
                     /locus_tag="LP452_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL2015329.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="LP452_002666"
     CDS             4799..5095
                     /locus_tag="LP452_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2015330.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="LP452_002667"
     CDS             5287..5907
                     /locus_tag="LP452_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL2015331.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         1751 a         1225 c         1096 g         1903 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggat
//
LOCUS       DAFELO010000108         5913 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFELO010000108
VERSION     DAFELO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.113"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(368..1486)
                     /locus_tag="LP452_002668"
     CDS             complement(368..1486)
                     /locus_tag="LP452_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015332.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="LP452_002669"
     CDS             1957..3264
                     /locus_tag="LP452_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL2015333.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="LP452_002670"
     CDS             3383..4582
                     /locus_tag="LP452_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL2015334.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="LP452_002671"
     CDS             complement(4609..5877)
                     /locus_tag="LP452_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL2015335.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1817 a         1253 c         1057 g         1786 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcccatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaa
//
LOCUS       DAFELO010000109         5894 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFELO010000109
VERSION     DAFELO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.114"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            641..1375
                     /locus_tag="LP452_002672"
     CDS             641..1375
                     /locus_tag="LP452_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2015336.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="LP452_002673"
     CDS             1391..2560
                     /locus_tag="LP452_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2015337.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="LP452_002674"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="LP452_002674"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL2015338.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="LP452_002675"
     CDS             3650..4576
                     /locus_tag="LP452_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL2015339.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="LP452_002676"
     CDS             4595..5845
                     /locus_tag="LP452_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2015340.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1971 a          958 c         1207 g         1758 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagtatc
     5881 gccgtttgta taat
//
LOCUS       DAFELO010000110         5501 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFELO010000110
VERSION     DAFELO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.115"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            153..1142
                     /locus_tag="LP452_002677"
     CDS             153..1142
                     /locus_tag="LP452_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2015341.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1124..1585
                     /locus_tag="LP452_002678"
     CDS             1124..1585
                     /locus_tag="LP452_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2015342.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1635..2099
                     /locus_tag="LP452_002679"
     CDS             1635..2099
                     /locus_tag="LP452_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2015343.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2113..2862
                     /locus_tag="LP452_002680"
     CDS             2113..2862
                     /locus_tag="LP452_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2015344.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2862..3692
                     /locus_tag="LP452_002681"
     CDS             2862..3692
                     /locus_tag="LP452_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2015345.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3692..4339
                     /locus_tag="LP452_002682"
     CDS             3692..4339
                     /locus_tag="LP452_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2015346.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4437..5213)
                     /locus_tag="LP452_002683"
     CDS             complement(4437..5213)
                     /locus_tag="LP452_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2015347.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5295..5438
                     /locus_tag="LP452_002684"
                     /pseudo
     CDS             5295..5438
                     /locus_tag="LP452_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1882 a          747 c          979 g         1893 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa
       61 aaaatttttt ttgaaaaata gtattgcaaa ataaagcaat ttcatagata ataggtatcg
      121 taaagttata taactttata atacctatta gagtgaggga taaagaaatg agacaactac
      181 acaaagaaca gattgaaaca gcaatggcag cgttaaagga aaagacaatt gatcatgtat
      241 attttacagc atgtgggggt tcacaagccg ttttaatggc tggtcaatat ctatttgata
      301 aagaattaac tgtaccttgt catgtttata ctgctaacga atttaactat gctataccaa
      361 aaggattatc tgaaaattct ttagttatca cttgttccca ttctggcaca acaccagaaa
      421 ctgtagaagc aacaaaaaaa gcaagagaat ttggagcaat gactatttgt ttatctaatg
      481 aagaaggatc tcctctatgg gaagcagctg aatatcctgt tcactatgat tggggtaaag
      541 atgttgatgc tagtgattta aataaaggaa ttctttatgg tttattattt aatattttac
      601 ttgttttaga tggctctaaa aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg
      661 atcttgttat tgctgaaaag agcaaatttg aaataactgc taaagaatgg ggtaaaaacc
      721 aaaaacgaga taaaattatt tacacaattg gtagcggtat taattacgga gaaatttact
      781 ccacagcagc ttgctggttc atggaaatgc aatggattaa ttctaatgct attcattctg
      841 gggaattttt ccatggtcct tttgaaatca ctgattatga tgtaccattc atcttagtga
      901 aaagtattgg agatacaaga tttttagatc aacgggtaga agacttcact aaaaaattca
      961 gtaataaatt aatggtttta gaccaagctg attttgactt agaatctata gatagtgtag
     1021 cgaaagaata tattgcagct gtacttagtg gcgttgttat tcgccaaatg gtagaagcga
     1081 ttgcttttga aagaggccat tccttagatg ttagacgcta tatgtggcaa atgaaatatt
     1141 aattaatagg aggttgcaaa agatggcaaa tcaattattt cacattgtga ttacaacaca
     1201 ttctaccctt tgtgagggtt atttaaaggc aacaaaatta attttaatga atgacgccac
     1261 aggtattagt gctgttccat ttaaagaaaa tactagtcca gatgattttg aggagagtat
     1321 gaaagaaata attagtcagc atcatttgca acctctaatt attttaacgg atttaattgg
     1381 aggaacacct aataatgttt ctattaaaca gttgaacaac aatagaattc aaattattgc
     1441 tgggattaat ttacctcttt tactggagtt attaatggcc cagtcagctg gtaaatctat
     1501 agaagaaata gattttaagg aattactgaa taactctcaa aaaagtatgg ttcatattaa
     1561 ttcgctttta aaagatgtca gttaaggtgg caatttctat tctataaatt ttaaaaattg
     1621 aaggaggaaa tagaatgatt aaattagtgc gtgtagacca cagattactt catggacaag
     1681 taatattttc ttggactaaa cagtatgata ttaaccatat tattgtggct gataacaaag
     1741 ttccaggaga tccaatgtct gtgatggctt tgacaattgc taaacctgct gattgcaaat
     1801 tagacattgt taaaattagt gatgttaaag ggattatgga aacatcccca aatgataatt
     1861 acatgatttt aattaaagga cctaaggaag cttttgaatt agttcaaaat ttaccagaaa
     1921 ttaaagaaat taattttggc ggtgtgtcta agaaaagcga gtccaaacaa tatggaaagg
     1981 cagtcttttt aactcctgaa gaattagaag ccacaaaaaa aattcttgat caagaagtga
     2041 aaatatttgt gcaacaagtc ccatcttcaa gtattgaaaa tgtagatttt agtaagtaag
     2101 gggggaaaat aaatgttatt aaaagccatt ttattaggtt tagttggtgt ggtagctgtt
     2161 attgactcca ggttaattgg tcgacaaaat attggtagac cactaatttt gagtacactt
     2221 gccggacttg tgctaggtga cttgcataca gggattgttc ttggggcatc tttagaatta
     2281 atttctatgg gattcgtctc aattggggcg gctggacctc ctaatatgca acttggtagt
     2341 attattgcca ctgcttttgc aattttaact aatagtagta cagaaacagc cttaacaata
     2401 gctatcccag tggctgtagc tggtgaattt atgagtatta tcatgcgtat gataattgct
     2461 caatttggac atgctgcaga taaagccatt gaaaataata aatttagaaa agctcaaatt
     2521 attcatattt attggtcatt tatttttaat gcttttgttt actttattcc aatcttttta
     2581 acagtatatt ttggtgctga cgttgtggca gatttagtag ataaaattcc agaaatgatt
     2641 acagacgcct tgactgtctc tggaaatatg ctttccgcat tagggtttgc aatgctatta
     2701 agcacaatgt tgagtaaaaa attatatcct tactttattt ttggattctt tattgttgcc
     2761 tattcaggtc tttcactaat tggcgtgaca atttttgcag ctctgattgc ctttgtaatg
     2821 gatcaagtta aatatggaaa aagagaagag gcgcatggtt aatggaaaat agtacagaaa
     2881 aaaaacgttt aatcacaaaa aaagatcttc ataagatgtg tcttcgttca tggacaatca
     2941 tgtgttcgtg gaattatgaa agacaaatgc atatgggatt tatgtatggg atggcgccaa
     3001 ttttaaataa agtttataaa aatgatgaag agaaaaaaag agaagcttat actcgtcata
     3061 tggcattttt taactgtacg cctcaaatga ctccatttat tatgggactt tgtgcttcta
     3121 tggaagaaca aaatgctcaa gcagaagaag gaaactttaa tactgaatct atctccatga
     3181 ttaaaaccag tttaatgggt ccttttgctg gaattggtga ttcatttttc caaggtactt
     3241 taagaattat tacatttggg attggattgt cttttgctaa gcaaggtagt attttaggac
     3301 cgattttagc tgttggcctt tttgctatac cttctatttt agctgttttt tacggcggta
     3361 aattaggata tgagtctggc aataaatacc ttactaaatt gtatcaagaa ggtcttatgg
     3421 atcgagtaat gcaatttgct tctatcattg gtttggctgt agtaggtgga atggtggcta
     3481 gcatggtgcc aattgtcacc cctttaaaat tcactacagg gggaacagaa cttgttttac
     3541 aagacttact tgattccatt attcctggat tattaccact tgtatgtact ttgggaatat
     3601 ataaattgat taagaaaaat gttagtacaa atatgctctt attaggttgt attttggcag
     3661 ggtttgtttt aagtataata ggtatcttat aatgattaag gctctaattt ttgatatgga
     3721 cggagtttta attgatagcg agattatgta tcaaaagtgg gtatttaatt ttttaaaaca
     3781 agaaaattta agctatcctt tgtattctta tcaaaaaaag attggaacta cagcaagtat
     3841 ttttgatgat atagaaaagt acaatgaggg atgtaacagt ttagaattga agggaaaatt
     3901 tactaaatat tggaaatcgc aaaagattga ttacttggat atttttccta atatactaat
     3961 tgaacagctt gaatggttaa aaaagaaaaa aataaaaatg gcaattgctt cttccagtcc
     4021 aatggatact ataaatgaag ttgttaccac ttgtaaaata gagtcatatt ttgattgttt
     4081 aattagcgga agagatttgc cagaaagtaa acctaatcct actatattcc ttaaagcttc
     4141 agaacagctc agaattccag ttgaagaatg tctggtgata gaagattctt ataatggtat
     4201 aaaagctggt aaaagagcaa atatgaaggt tcttgcaatt aaagataaac gtttttctca
     4261 agatgtaagt ctagcagatg atgttattta cgatattaaa tatctgcgac aagaagtaca
     4321 ggtgaaattt ggaatataaa taagtttttt gatgtattta gagaatatta gtaaaaataa
     4381 aaatcatgcc caaatctaat caaaattgat tgctttgggt atgatttctt ttaatatcaa
     4441 tctattactt taggatctga atcattttta tgcaccgtat ctaaagtgat attttctgtg
     4501 acttcaataa cataactaat tctatcacaa cgaactgtgg attgagaata atgaaatggt
     4561 tgattatttt tatctttgcc aaccttttct aaaataaata gcggatctcc tactatacat
     4621 tgcaataagt cggaaagctc aggtgttgca gataaaccgt ttatttccat aatagagctc
     4681 gtagctttga catcatagtc atcttctaga agtttatata atgaagtata ttggctacct
     4741 ttttcaaata aagttggaaa tttttcttct gatgcgaaaa aattatcaat tcccatcggg
     4801 gcaccgtctt cccacattaa acgtttaata ttgtaaactc tatcagttac attgatttgt
     4861 aatttattag ctaaaaactc atcagccttt ataattttag aatgtaaaac agtatatcta
     4921 attttatgtc ctcgggtaga aagagaatcg ctaaatccat gaactccagc taattttaaa
     4981 ctttcttttt tttgagttac aaaggtccct ttccccctta atcgttcaac tgttccactt
     5041 tctgttaaaa gtttaaaagc ttttcgaatg gtaatcttac ttactccata aattttacat
     5101 aattcttctt ctgtaggtaa ttgctctcct ggaagatatt ttttttcacg aatatcgtta
     5161 cgtatagaag cttctacttg tagatataat ggtctattat cttgaaaatt catagtacac
     5221 tccctaaaaa gttatacttc attatatcac aatttattaa aataaaagat taagatataa
     5281 atataaatga actagtcttt gactaaccaa tgcccgcttt tgaaagaaaa caaattcatt
     5341 ctgttttaag taaaaatcct tatgttacca ctcattctga aggggatgag ccttatcgct
     5401 acttggttgt ggaaccaagc gataagacaa ttttataaat tcaatattaa ttaaaataaa
     5461 aacaaacaaa gtagattatt ttactttgtt tgttttttta t
//
LOCUS       DAFELO010000111         5486 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFELO010000111
VERSION     DAFELO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.116"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(126..772)
                     /locus_tag="LP452_002685"
                     /pseudo
     CDS             complement(126..772)
                     /locus_tag="LP452_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(1180..1683)
                     /locus_tag="LP452_002686"
     CDS             complement(1180..1683)
                     /locus_tag="LP452_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2015348.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="LP452_002687"
     CDS             complement(1806..2570)
                     /locus_tag="LP452_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL2015349.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="LP452_002688"
     CDS             2678..2953
                     /locus_tag="LP452_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL2015350.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3391..4341
                     /gene="yidC"
                     /locus_tag="LP452_002689"
     CDS             3391..4341
                     /gene="yidC"
                     /locus_tag="LP452_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL2015351.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4520..4720)
                     /locus_tag="LP452_002690"
     CDS             complement(4520..4720)
                     /locus_tag="LP452_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015352.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1611 a         1140 c          954 g         1781 t
ORIGIN      
        1 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
       61 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
      121 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
      181 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
      241 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag
      301 attgcacaga ttcccttatt tatcgttggt gataaagtac cgctaatccc ttttaatttt
      361 tcatcttcgt aatagaccat gaattttttc tgcgtacctg gatcattcca gccgatgtac
      421 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt cacttttcgg
      481 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
      541 tcctccaatg ctctttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
      601 aatgtatcga ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag ctggcatctg cggtaataag ccgaaacttc
     1081 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttatctat taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tggtgacata ggctgagcgg cttctttgac
     1261 tgcgcagtat ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat
     1321 cgttgcgccc cacatgtgct tcaaaatgat atctcgctca agatccgaca attcggtgat
     1381 cttttctgca ttctttacag caattcttgg atggatatac gcatgtgtgc cttcatcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat
     1861 tccgcatttc ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 cttaccccct gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg
     1981 tctaatgcgt gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc
     2041 attaaaggaa tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc
     2101 tgcatgctgc gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca
     2161 ccagccatat caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt
     2221 acattatcta acaacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc
     2281 atcactgcta agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc
     2341 gaaactagaa aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg
     2401 cctcgctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc
     2461 agatggaacc cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc
     2581 tccccatgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattaattt ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt
     2701 ccaaggacgc gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact
     2761 aggcgtgact ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg
     2821 caatgacgac atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga
     3001 aaaatagctc tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga
     3061 acaccgtcga taaggttatt cttgaggcgc tttgcctcaa aaattaagga cagcagtgaa
     3121 aaagctgatc tatcacttgc agtgagttca tttgactccg ctttttcggg cagaaaaaaa
     3181 gtttttagtt tattaaagta agaaagaaac acttggaatc attctacttt ttcctagtat
     3241 ttctttcttg attttttgtc tttttttcaa aatttttcgc tacgtaaaag aacgaatatt
     3301 tatatgaaat ttctatgaag ttgattgaat tccatgcgct tttttagtat agttaagttt
     3361 gtgtaaaaat tatagaagag gaagtattct atgaataaat ggaaaaactg gttattggga
     3421 tccggacttg tcagtatcct gctcttttta tctggctgtg tgcggatgga ttcaaacgga
     3481 aaccctgaca cttcgggaat cgtttatcga gtactcgtcc atcctatggg ccaagcgatt
     3541 acatatctag ttgaaaattt caattggtct tatggctggg cagttattgc aatgactgtg
     3601 atcgtccgta tcatcatctt gccactaggt attagtcaat cgaaaaaaac aatgatccaa
     3661 tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag cccaacaaaa attgaaagct
     3721 gcaacaacac gtgaagaaca aatggcagca caagcagaaa tgcagcaagt ataccgcgaa
     3781 aacggattga gtatgaccgg cggaatcggc tgtctgcctc tattgatcca aatgccgatc
     3841 ttctctgcct tgtactttac ggcacgttat acagaaggta tccgggaatc atctttctac
     3901 ggcatcgatt taggctcacc aagtatggta cttgttgcca ttgcaggggt cgcttatctg
     3961 cttcaaggat acatctcaac gatcggtatt cctgaagaac agaaaaagac gatgcgtacg
     4021 atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga tcagtgcgcc agccggtgtt
     4081 acactttact gggtagtcgg tggtgtgttc agctgtctgc aaacattcat cacaaacgtt
     4141 atgatgaaac cacgtttgaa agcacaagtg gcagaagagt tgaaacaaaa cccaccaaaa
     4201 caagtcgtca ctccaagaat tgatgtgaca gaatcagcta aaccagctga cagcaaacaa
     4261 gcaaccaaac aaatcaagca acccaaaaac agtacaaaca ataaaggccg caacgcagga
     4321 aagcagcaga agcggaagta gatagtaaat aagagtgtaa taaaaattca ttaacagttt
     4381 tagcaattaa aaatatcgac caagattttt gaaaaagaaa tctggtcgat atttttattt
     4441 aagtgaatgt ttaatttgaa tcatcgattg atcgctatct aattgcagca gcatcttttt
     4501 atgaagaaac aactttttct tatctatcaa cgtagacagg tgttgctgtt tcttctaata
     4561 gtgtacttaa ttccttaata ttttgttttc cttcttctaa catccaacta ctcgcttttt
     4621 cttcgccttc ggtaacaaaa ggcgccactc ccttcgccca tgttgctcgc ccacataaga
     4681 caccattctc cataaaggac aacgcggaga taccacccat ctaaataaca tccataaacc
     4741 tttctagtag aagaatagtg atagtatcca tccctttttc tactgggaat acaattacac
     4801 gattcaaagc ttgatacaca acatttccct tcttataaac cgctattgta accgaattgt
     4861 tttgaatcac ctttttggga tagaaactgg ctcctttccg caacttctta aatgttggtt
     4921 tcttttatac tttctttgta gcaactacaa atttcttcaa atctctaaat gtttgcataa
     4981 caaccgcctt tctgactttt actcaggatg gtatttaaaa atcagcaaaa taaggcttct
     5041 ctccatcttt tttatttcct agctgcttta aagacactct tgctgttttt taatctctat
     5101 caagggggct ctgaatctat tttgatgaac ttatcttatt aatttctcaa atgagctgtc
     5161 agacacaact gtattttttc aatctcatac cggacaacta tgccagctta aaaaaatttc
     5221 ctctggcaaa acttctttat tgaaaaatga caatgaaaaa agaccattct ttttgttgaa
     5281 tgctcattgt gtaaacatat ttcctgcgac tgctcttcga accattctca gtgcaatccc
     5341 taatttatct attaaatctt cttttagttc ctcatctttc atattcaaaa tttcctttgt
     5401 atgttacaca agtttgttct ctaaataacg cgaattgtaa aattaagtta gaaaaataaa
     5461 aaggcattta tggtacactc aaattg
//
LOCUS       DAFELO010000112         5391 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFELO010000112
VERSION     DAFELO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.117"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(203..565)
                     /locus_tag="LP452_002691"
     CDS             complement(203..565)
                     /locus_tag="LP452_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015353.1"
                     /translation="MKKTMLKVFVGLFLLTGIGGIFSKTVDAGSYYYDKSSGNYVINV
                     TNSETGKNLNSIAGKTPGSVTNKMRNAAIKRYGSIQNNRRYYFSYKNIVTPYGGKKGG
                     VSFQWTGRVINMNCTGRS"
     gene            661..1032
                     /locus_tag="LP452_002692"
     CDS             661..1032
                     /locus_tag="LP452_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2015354.1"
                     /translation="MNKNKYYPIYEDLKYKIQHGEYKENEKIPSENQLCNLYSSSRAT
                     IRKAIELLAIFGFVDRSQGKQTIVSSKKNWNKRFLTEEEYKKLVLFNLKSTELNEIIA
                     LSEKYSVLKKLVDFWNNNNLI"
     gene            2138..3070
                     /locus_tag="LP452_002693"
     CDS             2138..3070
                     /locus_tag="LP452_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL2015355.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKHEDLRIKLFNIEHNGELTQEEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEGTQKQALSNELKQDLIGNLQNLFD"
     gene            3086..3610
                     /locus_tag="LP452_002694"
     CDS             3086..3610
                     /locus_tag="LP452_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL2015356.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSKENRKLWFSKVGNKFVIND
                     IGQKAIKSMFYSENENLNRKLVSGKTKTENLKNANQVSELELVSLLNKQLDQKDKQLQ
                     EKDTQLKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            3986..4267
                     /locus_tag="LP452_002695"
     CDS             3986..4267
                     /locus_tag="LP452_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015357.1"
                     /translation="MDILVRKIDSKIISEIDRRCQELTTKTSKKWSRNTYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNEYVRTNNQLIETLIGGL"
     gene            4269..4685
                     /locus_tag="LP452_002696"
     CDS             4269..4685
                     /locus_tag="LP452_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015358.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISILSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNHQKETNQTFTAGNFDPTNFLKR"
BASE COUNT         2017 a          870 c          868 g         1636 t
ORIGIN      
        1 aaaaaaaact atgttggtac taggttctat ttctctagga gtattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaataattc caaaggggaa cttaaattaa atccaaatta
      121 ctacaatagt cctaatgaac ctatgtttat ttcagaaaag caaaacgacg gtcaggacaa
      181 ataatcctgc cgtcgttttg ctctaacttc taccagtaca attcatgttg attactcttc
      241 cagtccattg gaaacttacg ccaccctttt tacctccata tggcgtaact atgtttttat
      301 aagaaaaata ataacgtcta ttattttgaa ttgaaccgta tcttttaata gcagcatttc
      361 tcattttatt agtcactgat cctggagtct ttccagcaat agaatttaaa ttttttccag
      421 tttcagaatt tgtaacgtta ataacataat tcccactaga tttatcataa taataagaac
      481 cagcatcaac tgtttttgag aaaataccac ctataccagt taatagaaat agaccaacaa
      541 atacttttaa catagttttt ttcatataaa aacctccttt agtttaagaa tatactaata
      601 attataaaga aaacgtttta tattatcaat gttaaaataa taaaaaggag agcttgatct
      661 atgaacaaaa ataaatatta tcctatttat gaagatttaa aatataaaat tcagcatgga
      721 gaatataaag aaaatgaaaa aattcctagt gaaaatcaat tatgtaatct ctactctagt
      781 agtagagcaa ctatcagaaa agctatagaa ctattagcta tattcggatt tgtggataga
      841 tctcagggaa agcaaaccat tgtttcatct aaaaaaaact ggaataaaag atttttaacc
      901 gaagaagaat ataaaaaatt agttctattc aatttaaaaa gtactgaatt aaacgagatc
      961 attgctttat ctgaaaaata ttctgtatta aaaaaacttg ttgatttttg gaataacaat
     1021 aatttaattt aactagcaaa ttttctaaaa agataaaaaa agaagtgggc aatatgggaa
     1081 attaaaccac tacttttttg ttttgttgct ccagcaacgc ttagtcctaa attatccgaa
     1141 agataaaata acaaataaaa tgttcattta ccatataaat ggtcgctaaa ataagcccac
     1201 aaaaaagagc cattaaccct ttgattataa aggattaatg gttcttttat tcttcgaata
     1261 ttttctattt atttgataaa atcacgattt ttttgaattg tgctaccaaa ataaatgcct
     1321 gtatatcaat cattgctacc aaatatttct ctgttattta gcattacgct cccctattag
     1381 agtagggggc acagtatttt ctacctgttt tgggggtttg tcccacatag cattggtgtt
     1441 caagtgctcc gtttttctgt caaaaaggtt cttcctggtt tcatatgttt atgggcctta
     1501 atttgcattt taagttgttt tggtgtctga atcatataag tataccaaag gatgggcaaa
     1561 acagcttaaa tcgaatttta gaaagggttt atgacgttcc agggttgtta gggcttgccc
     1621 tgaccgccta taaggcgctt taagagcaag ataagggttt ttatttaact tgtcgtagaa
     1681 agccttaaac aagcatttag ggaataataa aggccttttt aggaacgggt tgaagcaaag
     1741 gagcctttag tcgactgcgc agtacaatgt gagagcgtag cgatcacacc tttttagcga
     1801 ccgtagggag ccagtttccc agtttaggaa aatgcgcgaa tctgtttgag gagaaaagga
     1861 gtaatttttt aggcgggaaa ttttgcactc cctcttttat aatctctttt taaatctctt
     1921 tttaaacctc tttaccgtcg tctgttccct tactctccca aggggtgtag cttttttagg
     1981 gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     2041 ttataggcat gaaattatct gttttaggta ttgttttaag acataatatg tcttaaaatt
     2101 atgtcaataa aattagttag actggagtat caaataaatg gcaaatgaaa ttgtaaaata
     2161 tgaaccagaa ttaaatacca ttcctttaag aaaatttacc ccaatagaaa tgaacttgtt
     2221 tttttctatt gtctctagaa tgcgtgataa aggtaatcaa aaaatacgct tttatttcag
     2281 tcagcttaaa gatttaagtg cttataagca aactgctggg gaagtcttta taaaagattt
     2341 aacaagaaca tacgatcatt taatggattt acgttttgga aaaaaaagca atggtggatt
     2401 aaccatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag tcgataaccc
     2461 ttatgtagat ataaaagttc acgaaaaagc tttgccttta cttaacaatt tagaaagttg
     2521 ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt
     2581 tagattgcta aaacaatacc gaactcaagg atgggctgaa ttttccaagg aagatttttt
     2641 agagctatta gatattccta aaagctatag acaaacaaac ataaatcagt ttgtactcaa
     2701 acccataaaa gaagaactaa ctccactgtt taaaggatta acgattcgta aaaaatatgg
     2761 taaaggacga ggaaagcctg ttattggtta tcggtttact tggaaagctg aaattaatca
     2821 tgctgacgat ttctcaaaag gaaaacatga agatttacgt ataaaattat tcaatattga
     2881 gcacaatgga gaattaactc aagaagaaaa atggcgcgct aaagatcgaa tattaaatct
     2941 tcccttaggc actcatgaag cagattttaa aaagcagcaa caaactgaaa aagaaggaac
     3001 acaaaaacaa gcactctcaa acgaactcaa acaagattta ataggaaatt tacaaaactt
     3061 attcgattaa atataaggag tatttatggc agataaaact atcaaagaac ttgctgaaga
     3121 attaggggtt agtaaaacag caatcaataa aaaagtttct aaagaaaatc gaaaactttg
     3181 gttttcaaaa gtcggaaaca agtttgtgat taacgatatc ggtcagaaag ctattaaatc
     3241 aatgttttat tccgaaaacg aaaacttaaa tcgaaaacta gttagcggaa aaacaaaaac
     3301 cgaaaacttg aaaaacgcta accaagtttc cgaattagaa cttgtatctt tattgaataa
     3361 gcaattagac cagaaagaca aacagttaca agaaaaagat actcaactaa aacaaatgca
     3421 aaaattactc gatcagcaac aacaactcac cctgcaagcc aaccaacaaa tcgagaaact
     3481 gcaggaacaa ctacagttga cttatacgga agaatcttcg gaaaataaat caactactct
     3541 ttctgaagaa acagaatcga tagaaaaaca atcaaagacg gagaaaaaat ggtggcaatt
     3601 ctggcaataa aaccagcaat ttttattaat aaaaattgct ggttttattc ttctttttgc
     3661 ttttgcgagc agagcaacat gggcccatgt tgcgatattt tggtagcgcc ccaatccccg
     3721 ctttttcgtt gaatcttact cagactgcac tcgttttata gctgattttt ctgaattttg
     3781 aaagagcata aggaaacgtg gtatcacttt cttacgaaag gtgatagata ggctgtgcct
     3841 atcacatttc taagaaaaaa atttgctatc acttttttgc cattatggat agtgtatgta
     3901 tagtattttg gtaaaaaaca gctatacaaa tactatattt gtatgatata catagattat
     3961 acgcacatgc gaaaggaaaa ttgctatgga tattttagtt cggaagattg attcaaaaat
     4021 tatttcagaa atagatagac gttgccaaga acttacaacc aaaacgagca aaaaatggag
     4081 ccgtaatacg tacctaaaag ttctaatcga aaatgatttt gaccaggcac ttaggcatta
     4141 caaaaaagat caattcgatt tattactcga aaaatttatc gatatacaag caaaaaatac
     4201 ggaaacactg aatgaatatg ttcgtacaaa caaccaactc attgaaacac ttatcggagg
     4261 actataacat gagaaaaact tctgttcaaa aacacgttac cctttatccc agtcacgtgg
     4321 acgttttaga ttccattatt gaaagaaatc cagatatcaa caattactcg gaagccattc
     4381 gttttttatg tatgagttat gaacctgata ccaacacaac cactactagc aaccaagcca
     4441 aactaaatgc tatgggaaaa gaaatttcta tcctgtccga aatcattgca gaatttgctg
     4501 atttatatct aaaagatact ggtgttctta ttggtaccaa ttctaaggtg tatcaagaag
     4561 tgaaagaaaa aattgaaaat caactcaatc cttcccgacg aaatcaaccc aaaaatcatc
     4621 aaaaagaaac caatcaaaca ttcactgctg gaaattttga cccaaccaac tttctaaaaa
     4681 gataaaaaca gaacctgaaa aataaaaaaa ttcctcgatt tctttttagg gggggcggtc
     4741 ttccagcaag atttgcaagt tgggaccaac ttgcggaaaa aaagaaaaat tcgcttgcgc
     4801 gaatccttct ttttgcattt tggggcgctg ccccaatccc caagtatcgg ttatttaata
     4861 gcgtggcaac tcaaagaaca gaacatactc tgaaacaaaa aaagactgct ctaacgagca
     4921 gccagttaaa aaataatgag tgtttattct tgaaaaagca acgcgcttat gaagcgctac
     4981 caaaaattta tcatatttta ttaaaaataa caacccagta aaaaatgact ggtaaaaatt
     5041 cacaaagaaa cggaaagaag caaccgcaga aaatcaagct taaaaagagc tttttaccta
     5101 gaccaatgaa aaatcacaaa gtccgcccct gtacctgttg gtagctgggc tacttggcat
     5161 actgtacatt atgttaactt gaatttaaaa taacatgtat tattgtatta taaagcggac
     5221 tctattgaaa tctataattt tcacttttaa atattacaaa aaatcatata atttgagtta
     5281 ttattgttta gaagtgattt ttacaattta aacatttgac aaactataga acagcactta
     5341 aaatgtgtat acaagtgaca gcgctttaaa aaaggaggtg cagcctatgt t
//
LOCUS       DAFELO010000113         5255 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFELO010000113
VERSION     DAFELO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.119"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(46..366)
                     /locus_tag="LP452_002697"
     CDS             complement(46..366)
                     /locus_tag="LP452_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL2015359.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="LP452_002698"
     CDS             complement(514..2748)
                     /locus_tag="LP452_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2015360.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQKAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="LP452_002699"
     CDS             3227..3493
                     /locus_tag="LP452_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL2015361.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="LP452_002700"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="LP452_002700"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL2015362.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
BASE COUNT         1580 a          983 c         1057 g         1635 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttc
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt tttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaaga
//
LOCUS       DAFELO010000114         4948 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFELO010000114
VERSION     DAFELO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.120"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..156
                     /locus_tag="LP452_002701"
     CDS             <1..156
                     /locus_tag="LP452_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL2015363.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="LP452_002702"
     CDS             175..1452
                     /locus_tag="LP452_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015364.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="LP452_002703"
     CDS             1462..2118
                     /locus_tag="LP452_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015365.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="LP452_002704"
     CDS             2118..3494
                     /locus_tag="LP452_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015366.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3780..>4948)
                     /locus_tag="LP452_002705"
     CDS             complement(3780..>4948)
                     /locus_tag="LP452_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2015367.1"
                     /translation="NANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLE
                     KEVKLKERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQV
                     AFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNE
                     FLYDEYFDCYICPQDQMLAFSTVTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVIT
                     RHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATH
                     TMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGD
                     ISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1853 a          631 c          876 g         1588 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     4201 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg aagatgttcc
     4261 acttcatcca tcagatctcc ccaaacatgt ctcgtgatga ctcgttgatg atttttactt
     4321 ttggtacatt ggtttagcag aggacagttc acacattctt ttggattgga tttgtattct
     4381 cggtaccttt ctcgagtaac cgttgaaaaa gcgagcattt gatcttgcgg acaaatataa
     4441 caatcaaaat actcgtcata gagaaactca ttttttgata atagcccctt tttccctttg
     4501 ggccttgaat aaggcaaaca aggtcgaagg ttcaaatgag tcaaaaagtg aacaaactta
     4561 ggtgtcttat acccagcatc agcgacaaca caattgattt caggaaaaag tctcttcgat
     4621 tgtttgacga gctgaaaggc gacttgactg tcatgaatat ttccaggagt gatgattgtg
     4681 ctaagaataa atccattgtc gtcgcatgaa gtatgggcag aataagcaaa ttgtttttct
     4741 cgttcgcctt tgacgtagta gccgctttcc ggatcagctt tacttatttt tctttctttc
     4801 agcttcactt ccttttctaa gtccaaagta aaagggcgtt ttccagcctc tattcgttga
     4861 cgattgatct catcttctaa ttcatcctga taggctttcg cttcatcatg ggtcatttct
     4921 ttcgtaaact tatgtttatt ggcgttag
//
LOCUS       DAFELO010000115         4861 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFELO010000115
VERSION     DAFELO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.121"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..440
                     /locus_tag="LP452_002706"
     CDS             <1..440
                     /locus_tag="LP452_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBL2015368.1"
                     /translation="TFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATR
                     HLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSV
                     RGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            456..605
                     /locus_tag="LP452_002707"
     CDS             456..605
                     /locus_tag="LP452_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015369.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            681..1274
                     /locus_tag="LP452_002708"
     CDS             681..1274
                     /locus_tag="LP452_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL2015370.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            1287..2186
                     /locus_tag="LP452_002709"
     CDS             1287..2186
                     /locus_tag="LP452_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL2015371.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            2189..2662
                     /gene="ssb"
                     /locus_tag="LP452_002710"
     CDS             2189..2662
                     /gene="ssb"
                     /locus_tag="LP452_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2015372.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            3028..3288
                     /locus_tag="LP452_002711"
     CDS             3028..3288
                     /locus_tag="LP452_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015373.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            3733..3939
                     /locus_tag="LP452_002712"
     CDS             3733..3939
                     /locus_tag="LP452_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2015374.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3939..4190
                     /locus_tag="LP452_002713"
     CDS             3939..4190
                     /locus_tag="LP452_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015375.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            4206..4622
                     /locus_tag="LP452_002714"
     CDS             4206..4622
                     /locus_tag="LP452_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015376.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            4604..>4861
                     /locus_tag="LP452_002715"
     CDS             4604..>4861
                     /locus_tag="LP452_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015377.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVE"
BASE COUNT         1815 a          808 c          965 g         1273 t
ORIGIN      
        1 ctactttctt agctaatatc caaaaatttg tctctcactt gttggaagtc gtaccgggac
       61 aaatccagtc aacagatttt ggatcaacgt tacaagaggt gaaagcagcc agtgaaaaac
      121 aggaagcaac gcgtcattta ggtgtttgtc caaaatgtcg ggaacaagaa gtcttgcttt
      181 atcaaaatgt ggcggcatgt acgtctgaag cctgtgattt taaattatgg acaacgattg
      241 ccaaaaagaa actcacagcc acacaactga aagagatcat tcaaaacggc cgaacaagcc
      301 aatcagtcag aggactaaaa ggtcagaaag gttcgtttga agcaacgatc gttttaaaag
      361 aagactttac aacaggcttt gaattttctg aaaagaaaaa aacgaactat aagaaaagaa
      421 cacgccgcac tacaaaataa tgaaggggtt atcctatgcg aaatacaaca cacaaacaac
      481 aagaaatcga aaatcaaaaa gcaaaaattc gtttagaaca agagaaatta cgtgcaaata
      541 aaagaagact gcaacaatta aagaaccagc agcgcattca aaaagaaatc gaattagatc
      601 gatagacgtt aatcttgtta caaataaact actatgatat atttgatgta gtgtatttgt
      661 aacgaaggga ggggaagtaa atgcaagata aactgaaaga attgttggct attcgcgatg
      721 gaaatttatc tatgaaggaa gccaggaaat ttggaatcgc agcaaccact gtacaaagat
      781 tggtgaatca aggaaaattg atcaaagttg atcgtggtta ttatgtagag gatggtcatg
      841 gaatagatga tttattttgg cttcagcaac gattttccag gggaatcttc tctcatgaaa
      901 cgactttaga tatttaccag ctttctacga atatgccgaa attcattcac ttaacttttc
      961 cccatggcta taacgttgat cgttcaatta caaaagagca gcagttacaa tttcatttcg
     1021 tgaagaaaga agtttatgaa ctaggaaaag tagagggaac ttcctttcaa ggaaatccta
     1081 ttactatgta tgataaagag cgtacacttt gtgatatttg gaatccgaga tatgaatttg
     1141 aatatgaaat gaaattagaa gcattaaaag agtacatgga agacccattg agagatccaa
     1201 gtaaattaag agattacatg acaaaactac atgtagaaaa agaaatgaaa taccacatgc
     1261 aaagcttata ttgatggagg aaaaagatga caccgacaca atttgcagca aaaacaagaa
     1321 atttagcaaa gagtacagga ttaaatgcac agttagtgca acggcatttt atgatggatc
     1381 gtctaattga acaaatttct gaatcaaagt acaaaaatga cttcattttg aaaggcggct
     1441 ttttattagg atcaaaatat ggaatcgata gaaggacaac aattgatatc gatacgacat
     1501 ttagaaatag caaattaact gaagaaaaat tgaagagcat ttttaatgat ttaactagaa
     1561 aacccacaaa agaaggcatt gttttctcta ttcaaggaat gaaggaaaca agagaagccg
     1621 atttttatcc tggatttcaa attaaattgg tggctaattt agagaaagta cgtgtaccgt
     1681 ttaaaatgga cgtgacaaca ggagattcta tttatcctga agtagataca cactatcacc
     1741 aattaatgtt tgaagataaa aaagtggaga ttccagctta tccaacagaa caaattattg
     1801 cagaaaagct aagtgctacg ttcagctttg gcacagataa ttcgagagca aaagattact
     1861 acgacttatt taccattccg aaactagaaa atatcgatac gaaagagtta tacaaaagtg
     1921 ttcgaaatac atttacaaaa cgtggggata caaaaccgtt gtccttttat tatgaaagag
     1981 aaatggataa gatcagagga aatgactatt tggaaaagca gtgggaaaaa tatcgctcag
     2041 ttaacacgtt tgcgaattcc atttcattta cagatacagt caacgaaatt gatcacctaa
     2101 tgaaaagtgt gatcaaaatt gaaaatcaag aaagaaaacg gcagtttaaa agatcacaac
     2161 aaatgaatca agaaatggag agataaagat gattaataat gtgacattag taggacgctt
     2221 aaccaaagat cccgatttac gttatactgc aagtggcaca gcagtagcga cttttaccct
     2281 agccgtcaac cgaaatttcg cgaatcaaaa cggcaatcga gaagccgatt ttattaactg
     2341 cgtgatttgg cgcaaaccag cagaaacaat ggcgacatta gctaaaaaag gcggcttgat
     2401 cggtgttgtc ggacggattc aaacacgaac ttatgacaac cagcaaggac aacgtgtgta
     2461 tgtgactgaa gttgtagcag ataactttca actattagaa agtaaagcag cgacagagag
     2521 ccgtgcgcac gctgatcaat caaccaccag tccaagcccc actacctttg aacaacggga
     2581 tacaaacaat ggattggacg cttcacaaaa tccatttggt ggacaatcaa tcgatattag
     2641 cgatgatgat ctaccattct aagtagcatt ttctattggt tgcatttgct acggtttatg
     2701 ttataattta gtcaacaaaa agaattttca atctataacc aaaaaccact agctggcggg
     2761 ctagtggttt tcttttacgt ttctattata agaattaacg ggtaggctta ctgtctttct
     2821 tggcaaattt acaatcaagc catttgcaga tatagtgcgc ggctacttct gccatgattg
     2881 caaaaacaaa atcaaaaaga attttcaaac ctataacctc ctccccttgc cagattaggc
     2941 cggggtagca gacagcaaga ctattatagc ataattgaaa tattcaaatg attggatttt
     3001 tgctataatt ttagcgaggt gattgtgatg aacaaatatg ttggtttgct tgataaaatc
     3061 cgagtaataa aaacgcaacc gttattggtt cgctttactt tacaaacgat tcatgaatcg
     3121 attaattgtg tagttgcaga tattgaaatt atcgataaat tgctaattat ggatgatgga
     3181 aaatacaata ttgccgtcac aggtcacttc aataaacgaa accagctagt tattgctagc
     3241 atgtatgtta gaaatcccga tcagtttacg cgattgatgg gcatataaaa aaagaagagt
     3301 tgcaatccta atgtaggact gcagctcttt ttgttttaca tagaaacaag aaaaatgaaa
     3361 aggccttttt aggaaggcgc gagcgccttc tcaattgaaa aacctctctc actccaaccg
     3421 ccaccgccgt tcgagaagaa aggaagtgaa cgttgcacgt tcactaagat aaagggtctt
     3481 gcaaagcaag acaaaataga aaaaacttcc tctcaccaaa aggttcgttc ctgttcgtta
     3541 tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa aagcgacaag
     3601 gacaagggta aatggcacgc tgcgcgccct tgtccttgtc gcttttcgtt gattcattct
     3661 ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc ctaagaggag
     3721 gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg ttcccttcag cgaattattc
     3781 ccaaactatc atgcaccaca tactgtcgaa tgcgattgtg gagaaagagc gaaatgtatc
     3841 aaaagctatc gtttgtatca atgtccaaca tgcgggaaaa aatatacctt gtcttatggc
     3901 gattatattt tgattgatga gaaaggaaag aaacgttgat gaaaacacaa cacaaattat
     3961 tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct gaaaaagtag
     4021 cagaagcttt acttattccg cggtactgga cgacagaaac ccctttagta gaggatgcac
     4081 cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat tatcaagaaa
     4141 acttggaaac ttatgaaaac gaatattggt taaccgaatt agagaactaa aaaggagaaa
     4201 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
     4261 aaaaaatgga ggatatgaca ttcctataca atgagacacg tgttccaaaa aaacattata
     4321 aggaaagatt gtcggtagct gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
     4381 ttgcgacgta ttatcgaaca ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc
     4441 aagccttact atgcttggaa gtaggcgtca aaccaagcac aatcaaacct tctgaatacc
     4501 aagctttaga actaacgtat gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa
     4561 gtgaatggtt agattatttt gaaaatatca ataagtatgg cgcatgttac atcatgaagt
     4621 aatcagacgg attcaaaaag actttagttg tatggaagaa atgaagtact ttgttcaaaa
     4681 tgttgaagaa cagttatatt tggaaagcga aacgagtcaa acaaaggtca cagtcagcca
     4741 taatcgttta gatgtgtata aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct
     4801 acgactagaa acagaaaaat acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga
     4861 a
//
LOCUS       DAFELO010000116         4807 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFELO010000116
VERSION     DAFELO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.122"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LP452_002716"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LP452_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL2015378.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="LP452_002717"
     CDS             complement(3250..3768)
                     /locus_tag="LP452_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL2015379.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          787 c          730 g         1711 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc tcaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DAFELO010000117         4791 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFELO010000117
VERSION     DAFELO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.123"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(75..1073)
                     /locus_tag="LP452_002718"
     CDS             complement(75..1073)
                     /locus_tag="LP452_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL2015380.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LP452_002719"
     CDS             complement(1148..1444)
                     /locus_tag="LP452_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2015381.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LP452_002720"
     CDS             complement(1437..1730)
                     /locus_tag="LP452_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015382.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LP452_002721"
     CDS             complement(1876..2427)
                     /locus_tag="LP452_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBL2015383.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LP452_002722"
     CDS             complement(2601..2900)
                     /locus_tag="LP452_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL2015384.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="LP452_002723"
     CDS             complement(2927..3235)
                     /locus_tag="LP452_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL2015385.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
     gene            3540..4223
                     /locus_tag="LP452_002724"
     CDS             3540..4223
                     /locus_tag="LP452_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015386.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="LP452_002725"
     CDS             complement(4260..4757)
                     /locus_tag="LP452_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2015387.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1424 a          908 c          700 g         1759 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcga aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DAFELO010000118         4616 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFELO010000118
VERSION     DAFELO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.124"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(15..962)
                     /locus_tag="LP452_002726"
     CDS             complement(15..962)
                     /locus_tag="LP452_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL2015388.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="LP452_002727"
     CDS             complement(1140..1943)
                     /locus_tag="LP452_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL2015389.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="LP452_002728"
     CDS             2089..3003
                     /locus_tag="LP452_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL2015390.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="LP452_002729"
     CDS             3051..3521
                     /locus_tag="LP452_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL2015391.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="LP452_002730"
     CDS             3606..4214
                     /locus_tag="LP452_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL2015392.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="LP452_002731"
     CDS             4348..4548
                     /locus_tag="LP452_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2015393.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttag
//
LOCUS       DAFELO010000119         4494 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFELO010000119
VERSION     DAFELO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.126"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            104..367
                     /locus_tag="LP452_002732"
     CDS             104..367
                     /locus_tag="LP452_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBL2015394.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            361..711
                     /locus_tag="LP452_002733"
     CDS             361..711
                     /locus_tag="LP452_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2015395.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(735..1349)
                     /locus_tag="LP452_002734"
     CDS             complement(735..1349)
                     /locus_tag="LP452_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015396.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1663..2085
                     /locus_tag="LP452_002735"
     CDS             1663..2085
                     /locus_tag="LP452_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015397.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2095..2298
                     /locus_tag="LP452_002736"
     CDS             2095..2298
                     /locus_tag="LP452_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015398.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2509..3093
                     /locus_tag="LP452_002737"
     CDS             2509..3093
                     /locus_tag="LP452_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015399.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            3262..>4494
                     /locus_tag="LP452_002738"
     CDS             3262..>4494
                     /locus_tag="LP452_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2015400.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVH"
BASE COUNT         1567 a          678 c          853 g         1396 t
ORIGIN      
        1 gattcatgga atgttaatct aatttaatag aaaaggataa ttaagtatat acgttttttc
       61 ttatgtatgt tatactatgt atatacagaa agaggtgtct actatggaaa tgttacctat
      121 aaaaaaatgg ggaaatagta atggccttag attgccaaag tatattatgg aatatcttgg
      181 aattcataca gaagacaaag tgaagataat acaagaagaa gtaaacggtc agaagcggct
      241 aattatcgaa gcggctaata tagaaaatga attaacaatt gaacagttat ttgaaaatta
      301 cactgatgaa agaaatcatg tgacgattca aaatttaggt gaagcagtag gtaatgaaaa
      361 atggtgagaa aacctagaca aggcgatatt ctattattga atacagcacc tcgatctgga
      421 cacgaacaga caggtaaaag accttatatt gttttaagtc atgatattat tgcggattat
      481 agtaacgttg ttacagttgc tccaatttct agtacaacta gaaactatcc gctatatgta
      541 aatataaact cagaatatga aatgaagaca acaggaaaag tattattaga tcaactaacg
      601 acaattgatt atgaagccag agagtgtatt tttttggaaa aagcgcatga tagtttagtt
      661 gaggagttat tgatgaaagt tagaacagtt tttcaaaaag taagcaaata aaatagaaac
      721 agcagcatga tcaactattc gtgattgacc atgctgtctt tttttattct atatacggtt
      781 tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg caacaccttt ttttagatcc
      841 tcaacgatgt ttttataaac aagacgacgt tggggatctt ttgcattggc actgtatagc
      901 ttaggacgtc ctttataaac accgttcgct tttgcaatct taatgccttg tgcctgacgt
      961 cgtttagatt cggtacgttc ttgttcagcg atcatggcca aaatttgaat gatcaaatct
     1021 ttaatgaatt tatccaggag aggattgcca attgcttcgg ccatgatcgg tagacttgtg
     1081 atgatcagct gcacattttt cttcttcaag taattgactg aagcgatgat ctcgtcgtaa
     1141 tttcttccaa gtcgatcaat agcttccaca ataaaaatat cttgatctcg gacgaaatcc
     1201 agtgcttctt gaaaaacagg tcgatgagta atcgttgcgc cagattgttt ctcaatgaaa
     1261 attctctctg caccgaattt tttcatttct tcaatctgcc gttgttcatt ttgatcgatg
     1321 gatgagacac gtatgtatgc aactctcaaa atttttacct actttctgca actatttaaa
     1381 aaacacccta atcatacacg agaaaaccag atttttcaat gtcgcggtcg tgtctagtta
     1441 gggtatacct tatatagaca aaaactaatg atggaggaaa tagtatgtgt cacttttctt
     1501 tttaaatggc aatatttttt gtaaataaat gtcaatcaag aaaatttaaa cgattatgac
     1561 attaaaaata tattttatga attttctagt atataacttg atttatctgt taagatttat
     1621 taagaaaacg ttttcttatt tttgatttcg aggtgataga caatgaaaaa taagtttatt
     1681 ttatctttag gtattttaac tattggaatt agtgtaactg gaactacaat tcctacattt
     1741 gctgatagta caaatgaacc aactactgta gtagataata accaagaatt aactccttta
     1801 gataggaacg gaatagccat tggtaaccca actaacgact taactgttac tgaaaattat
     1861 ttaatagttt ctccattact tgctcgagca gcttctaaaa cgatacgagt gacaggaacg
     1921 atgatttttg ataaagttgg ttggccttct agacctcctc aatcacggaa ttggaaagaa
     1981 aaaagaaatg gttattggta ctatggaaaa ttgtatttac aaagttataa atcagctgga
     2041 aacaattgga ttgcgactta ttcaggaacc ttaactagaa agtagggatt ttttatgcca
     2101 gaatttgtga acacaccaaa tttttatcaa gatagcgata ttctaagtaa aattaattta
     2161 ccgaaagaag aatcagcttt acaagttcag cgaactttac aggagacaca aaaaattcaa
     2221 aaatcaattt ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga ccaaaaaaca
     2281 tatcaaaaag aaaaataatg attttaaaaa aagagtgtct agtcgacatt ctttttttct
     2341 aattgaaagt gttcataaaa tcacccattt ctggacactt tctttttttg ctttattttt
     2401 caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt cagttaaact
     2461 atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt gaaaatagga
     2521 tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag cctaaataaa
     2581 tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa tcgaccagga
     2641 ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg gcgcttagat
     2701 cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa tgagcgtggt
     2761 gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc tacaggacag
     2821 ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat cctggaacga
     2881 tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg tcctgaaaaa
     2941 ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac acctattaag
     3001 actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt ggcaaaaatg
     3061 gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc tgaaaatttt
     3121 tgccatttat ctagaaaagt gacaatagta tgagaatttt ttgtaagtgt ctactgataa
     3181 cttattctca aatatttagc tatttcttct ttacaacatc gaacatatgt ttgtataatg
     3241 tggaagaatg gagtgttatt tatgatggat tattcaaaag agccagtaaa tgattacttt
     3301 ttaatcgata tgaaaagctt ttacgcaagt gtcgaatgta tagaaagaaa tttagatcca
     3361 ttaacaacag aacttgttgt tatgagtcga tctgataata ctggttcagg attgatatta
     3421 gcttcttctc ctgaagcaaa aaagcggtat ggtattacaa atgtgagtag accacgtgac
     3481 ttaccacaac catttcctaa aacactacac gttgttccac cacgtatgaa cctatatatc
     3541 aagcgaaata tgcaggtaaa taatattttc agaagatatg tggctgatga agatctactg
     3601 atttactcga tcgatgaatc gattttaaaa gtgacaaaat cactgaatct ttttacgact
     3661 gaagaaacac gaagtcagcg aaggaagaag ctcgctcaaa tgatccaaga acgtattaaa
     3721 gaagagttag gtttgattgc tacggtaggc gtcggcgata atcccctttt ggctaaatta
     3781 gccttagaca atgaagcaaa gcataatgaa ggatttattg cggaatggac gtacgaaaac
     3841 gttcctgaga aagtatggaa tattcccgaa atgacggact tttggggaat tggttcgcga
     3901 atgaaaaaaa ggctcaacca aatgggaatt ttgagcatta gagaccttgc taactggaat
     3961 ccttatacga ttaagaatag attaggtgtg attggattac agctgtactt ccatgctaat
     4021 gggatcgatc gaacagatat agcaatccca ccagaaccga caaaggaaaa atcatatgga
     4081 aattcacaag tgttaccacg agattatact agaagaaatg aaatcgagct ggtggtaaaa
     4141 gaaatggcgg aacaagtagc gatacgcatt aggcagcata actgtaaaac aggttgtgtc
     4201 catttacaca ttggcacgtc gatcttagaa acgagaccag ggttttcaca ccagatgaag
     4261 atccctatca cagataatac aaaggaattg cagaattatt gtcttttctt gtttgataag
     4321 tattatgaag gacaagaagt aaggcacgtt ggaataacct atagcaagct tgtgtacacc
     4381 gattcgcttc agctggattt atttagtgat ccacaaaagc aaattaacga agaaaattta
     4441 gataaaatta ttgacaagat tcggcaaaaa tatggtttta cgtcgattgt acat
//
LOCUS       DAFELO010000120         4462 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFELO010000120
VERSION     DAFELO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.127"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(306..836)
                     /locus_tag="LP452_002739"
     CDS             complement(306..836)
                     /locus_tag="LP452_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2015401.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1018..1674)
                     /locus_tag="LP452_002740"
     CDS             complement(1018..1674)
                     /locus_tag="LP452_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL2015402.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1801..2274)
                     /gene="dfrF"
                     /locus_tag="LP452_002741"
     CDS             complement(1801..2274)
                     /gene="dfrF"
                     /locus_tag="LP452_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL2015403.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2693..2911)
                     /locus_tag="LP452_002742"
                     /pseudo
     CDS             complement(2693..2911)
                     /locus_tag="LP452_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2942..4156)
                     /locus_tag="LP452_002743"
     CDS             complement(2942..4156)
                     /locus_tag="LP452_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL2015404.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1373 a          944 c          658 g         1487 t
ORIGIN      
        1 gaaatcaact gtctttctca tatttactat tcaattttca tagtgatata ggtacaattt
       61 atatcactac tgtaaaaatg gggttttgcc ttataaatac agcgttttcc gcttgttagt
      121 gtatagtgtt tcttgttttt tgtgcccccc ttgttagata acgggggctt tgaacggctc
      181 gcaagctcgc cgaaccgccg aagcgaaccg cccccttgca agcatggcgg aaaaatctta
      241 aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt tctgtttctc
      301 tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg tgttttacaa
      361 agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt tcctaacaga
      421 gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa ctgctgaaca
      481 agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag ccgttccaat
      541 aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca ctctttatgt
      601 gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag ttcagtaaca
      661 acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt aaattctgta
      721 taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac tccaccatta
      781 ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt tctcattttt
      841 tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag ggaacaactg
      901 attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa atcactaacc
      961 atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa tttttgccta
     1021 tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc aatgtcttga
     1081 aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa tcagccaaat
     1141 tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc tttctttcgg
     1201 tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag tgctttccca
     1261 tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa agtctgcaat
     1321 ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac gctcttgata
     1381 attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa ctatatcttc
     1441 aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac catcagcgtt
     1501 tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag atactaaggt
     1561 ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat aagtatcttt
     1621 cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat tcatattcaa
     1681 tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct tacaacaatt
     1741 tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa tttactcaat
     1801 tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa gtgtttcccc
     1861 tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc catcttcaat
     1921 atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt ccttaaatag
     1981 tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg catcttctaa
     2041 tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa caacaatatt
     2101 cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca taataaccac
     2161 attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc atggtatatt
     2221 accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta tcatctattt
     2281 catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca tcttaacaaa
     2341 ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc atctgcatta
     2401 tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta cttatcagcc
     2461 tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg acaaaccata
     2521 gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt cacggcaaca
     2581 aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat attcttttgt
     2641 ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa acaccttttt
     2701 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
     2761 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
     2821 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
     2881 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
     2941 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
     3001 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
     3061 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
     3121 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
     3181 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
     3241 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
     3301 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
     3361 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
     3421 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
     3481 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
     3541 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
     3601 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
     3661 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
     3721 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
     3781 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
     3841 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
     3901 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
     3961 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
     4021 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
     4081 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga
     4141 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
     4201 agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt
     4261 aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata
     4321 aacggtcttc tttttataga aaaccgttta ttttcgtctt tttttatttt taaagatatt
     4381 ttttcataag gaataaagaa tacttcatat aaaatatata aaaaagaacc gcagcgagtg
     4441 tcgctacggt tctcaaattt gt
//
LOCUS       DAFELO010000121         4434 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFELO010000121
VERSION     DAFELO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.128"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(543..2048)
                     /locus_tag="LP452_002744"
     CDS             complement(543..2048)
                     /locus_tag="LP452_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2015405.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LP452_002745"
     CDS             complement(2045..2731)
                     /locus_tag="LP452_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2015406.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LP452_002746"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LP452_002746"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL2015407.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAFELO010000122         4400 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFELO010000122
VERSION     DAFELO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.129"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(97..1617)
                     /locus_tag="LP452_002747"
     CDS             complement(97..1617)
                     /locus_tag="LP452_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL2015408.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1860..2045)
                     /locus_tag="LP452_002748"
     CDS             complement(1860..2045)
                     /locus_tag="LP452_002748"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBL2015409.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2029..2211)
                     /locus_tag="LP452_002749"
     CDS             complement(2029..2211)
                     /locus_tag="LP452_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015410.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2452..2670
                     /locus_tag="LP452_002750"
                     /pseudo
     CDS             2452..2670
                     /locus_tag="LP452_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3165..3764
                     /locus_tag="LP452_002751"
     CDS             3165..3764
                     /locus_tag="LP452_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2015411.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3828..>4400
                     /locus_tag="LP452_002752"
     CDS             3828..>4400
                     /locus_tag="LP452_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBL2015412.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1402 a          799 c          785 g         1414 t
ORIGIN      
        1 aattcagtag ttttgagcgt aagcgtgtca tagaatggta cctaaacaca aatagcccga
       61 tttgatacac tcaaatcagg ttatttgtgg cttatattat ttacagattt cttgtatctg
      121 ctgattccat ggcaaataag cgcgtagcgc ttcaggtgtc atcgaagaca gattgggaat
      181 ctctgtaaaa agatgattta aatatttgcg gatgtttaac ccattcgcaa cagctgtctg
      241 gataatactc aatataattc catttgatct agcgcctttt aagcttttag aaaatagcca
      301 gttttttcga ccgatcacta actctttgat ggcacgctct gctcggttat ttgaaagttc
      361 caagcgtcca tcaagcaaca caatctgaag tccgtcttta tgattgactg cgtattgaac
      421 cgcctttcca agctgcgatt ttggcaaata agcaaaactc tcacaccaat gaaaaaattc
      481 agtcatcaca ggctgcaacg tttccttcct cgctacaaac ctttcttctg cgctaagctt
      541 attaaaggtc cgttcgattc gaaacatctt gtcacagaag tctaatccta ttttggcatg
      601 actttttggg gacggttgtt tacccaatgc ttcaaaaaac tttctgcgaa catgcgccca
      661 acaataaaca ttgtttaccg gcagctttcc ataggccgcg taaccatcag tatgcaagaa
      721 accgctaaat gattctaaaa aactttttgg ctcatcaacg gaacgcgaat ttgcgtgctc
      781 gtagagcaca acttgttttt cttcgctttt acctgaagca tataaccagt aaaaggtctt
      841 ttctttatga gtattcaata cccgatagct tgtctcatcc gcgtgtaaga cctcctgatt
      901 gcttaattct tgatgaaggc aattgtagat tggctctaaa accatttcac ttaccttgat
      961 atgccaatta gtgatttttt tacgatcaag agggaaacca agtgcatgcc attctttttc
     1021 ttgtcgatat gccggcacct ttttaacaaa tttttcccaa atactctgtg tcacaattga
     1081 agaggaacct agactattat tgatcaacgg tttaggaaca ggcgcttttt tgatttttgt
     1141 ttctcctaat gcttcacatt ctctgcaatg ataggcatgt tggtagatga cttgtttttc
     1201 tatatgggca ggaacataca atggctcttc tctaagcctt gtcgcaccga tatcatgtaa
     1261 agtatgacca cagtcttcac acaaacaatc ggcacctttt aattcataat gaataggaac
     1321 ttcaggcaga ccttctaatg aaagtgcttt gagtcccttg cgttttctgc gtttaaaaga
     1381 agataccgta atgggttcct ggctttcttg tccagtgtgc tctggttcat taaaagttga
     1441 gtcgtctaat aaatcgattt gcccatctac gggtggtgtt tctttttttc gtccaaacaa
     1501 gcgtcgattc atttcagcaa tttgttctct aagaagagta ttttcctgtt gctggatttc
     1561 aagcaactgt gttaattgct tgatttgtac aagaagttca gattccattt ttgtcatttt
     1621 tacaccacca ttctagcgat tttcttacat ctattatacc attttatgac aacgaaaaac
     1681 agacaaaatc cttgatttaa caaggttttc gtctaatata tcgcaccttt tttcgtttct
     1741 tttagcgtac atttttcttc gattgaaagg ccagtcagta agacttcttt tctggaacgc
     1801 ggccattgaa ggtacccact ttcaaatctt ttgtacatta atacaaatcc attttgatcc
     1861 tagtaaagag ccttgaagcg atctttcttc gtaccgcaaa ataagaaaag cgcttgatcg
     1921 taaacatcaa ggtcatattc ttctataacg aaagctgcta gtccatcaat tccttttcga
     1981 agatctgttt ttccacaaac gataaaaata tgtttcaatg cagttgcatc aatgagcatt
     2041 ggcattcaac tcttttaaca aggtatacaa aagatattta tccattccat tataaataaa
     2101 tatttcctta tcttccgatt tgattctgca agctagtttt ggcttggtgt caatttgcga
     2161 tttttgagcc gcacttgaaa attgaactgg tacaatatct tttttattca ttcgtttttc
     2221 tccttctata tttgatagtt taattataga agaaagataa tcggcgtacg agatactatc
     2281 ctatgacacg cttacattta atttaatatt ttgcctcaaa ttagccacac tttttataga
     2341 actaagagga aaaatgaagt tttcttccta ttaatatagt tgtttgaaat cttaaatata
     2401 gcgttttacg atgtaatggg aagtatggtg aataaatcaa ctatgttcgt acgaagcatt
     2461 tactcaacta atttaatcga atctttcaat aaacaaatca agaaatacag ccaccgcaag
     2521 gaacagttcc aaaatgaaga gtcgatggaa cgtttcttag tctcgtcttt tgatacttac
     2581 aaccaaaaat tcctaggtcg cagtcataaa ggtttccaac aggccgaagg cgagctcgaa
     2641 caaatgctga gtcaaccgat ggagaattag aaacaatcta cagtagggaa gaaccattga
     2701 cacaaaatta ttgacgctcc caactaaaag tgtcaaaata atgatgaaat catttagttc
     2761 ttataaatta gaaaagagaa aataaatatt ccatatctac tttattggat ccgtaacatg
     2821 actaaattct gaatactttt taaagcttat catttggtaa aactgaatat aagttaatta
     2881 tatagactga aaattttgaa ttttcagaat agaataactt aaaagtactg acaaaatttt
     2941 ttaaaggggc aatattaatg ttctgagata atcaaatcct accgaaaata tcaaaaatcc
     3001 aatgtgacac aatcagattg tgtctcattc gagggaatca cacaatccca cgtggctcta
     3061 ggcagaattg aatttttgct tggggctttt tccttatatt gattttgaat gagacttatt
     3121 atatgttata atgcaattaa gtatcattgg agagggcgaa gaaaatgaag acggtagaac
     3181 ctattcgaga caaaaaaaag atcgatgcga tgaaggcaat cttggcttcg ggaaaatatg
     3241 gccaacgaaa tttggttctt ttttcgattg ggatcaacac cgcctatcgc atttctgatt
     3301 tgcgtcaatt gaagctggcc gacgtgctgg aaatttcacg cggtcgcgtg gtggtcaaag
     3361 atcgattagc gatgaaggaa caaaaaacag ctaaacataa ttcggtctat atttccaaca
     3421 aattgcgaaa aacgattttg gagtacgttc aacaggaatt tcctgatcaa ctccttcagc
     3481 aagattttga cggttatttg ttccctagtc gaaaagggac gaatgaaccg ctcaaccgcc
     3541 aaagtctgtg gcgaatcatt catgatgctg gcacggcaat cggattgaaa gacattggcc
     3601 cacattccat gcgaaaaacg ttcggttact ttctctataa acaggggacc aaaacagaaa
     3661 tcattcagtc cttgttgaac cattcttctc agcgcgaaac attgcggtat attggtatta
     3721 cacaagaaga caaagataca gctgtaaaaa gcttagattt ataatataaa agttaagttt
     3781 aaacatggat agttttaaat tagtagattg agtgttggga ggaaagaatg agtacattat
     3841 atattatagg aaacggtttt gatttaagtc atggtttaaa aactagatac actgattttt
     3901 caaaatgggt tgaaaaaagt catagtgaaa tattcgataa aatagacaca atgataaatg
     3961 aaaataatga ggtgagaagt acagctgatg ataagatagg ttggtttaat tttgaaagta
     4021 gtttgcatga gttaattcaa agtccagtct ttgaagatta tgtagaagag tctaaggatt
     4081 atctcatagg ttatggacat gaagattggt cagattctgc ccatcatgat tttcaaaata
     4141 tggtttatga ttatgttgtt tctctagaag aattacaaca acttttcaag gaatggatac
     4201 gcaaagaaat acagccgaca atagaacata aaattccaaa ataccaattt tcgtccgatg
     4261 tactattctt tacattcaat tatacagatg ttttggaaat tctatacaag caaccgaaag
     4321 aaaacatata tcatattcat ggcagttatg acgatataat aatcggccac tccgagaatt
     4381 ttgacaactt tataggagat
//
LOCUS       DAFELO010000123         4334 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFELO010000123
VERSION     DAFELO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.130"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            127..579
                     /locus_tag="LP452_002753"
     CDS             127..579
                     /locus_tag="LP452_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015413.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="LP452_002754"
     CDS             593..1282
                     /locus_tag="LP452_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015414.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="LP452_002755"
     CDS             1407..3758
                     /locus_tag="LP452_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL2015415.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="LP452_002756"
     CDS             3838..4233
                     /locus_tag="LP452_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015416.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1393 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttat
//
LOCUS       DAFELO010000124         4316 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFELO010000124
VERSION     DAFELO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.131"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            148..1137
                     /locus_tag="LP452_002757"
     CDS             148..1137
                     /locus_tag="LP452_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL2015417.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="LP452_002758"
     CDS             1134..1553
                     /locus_tag="LP452_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015418.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1532..1870
                     /locus_tag="LP452_002759"
     CDS             1532..1870
                     /locus_tag="LP452_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015419.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1874..2236
                     /locus_tag="LP452_002760"
     CDS             1874..2236
                     /locus_tag="LP452_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015420.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNEYDGLLGVIPSKK
                     SQMNESKFMSIFNDVNLR"
     gene            2458..3864
                     /locus_tag="LP452_002761"
     CDS             2458..3864
                     /locus_tag="LP452_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL2015421.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELGYISRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKKAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1506 a          606 c          987 g         1217 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc ccgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc aaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gttatcgtca atttctgacg atcagcaaca cgaaaaatca gtggataatg agtggcttgc
     2101 tgatgaaaaa tcagcgccaa gcgaacgaga ttatcccaat gaatatgacg gcttactcgg
     2161 agtgataccg agtaaaaaaa gtcagatgaa tgagagtaaa tttatgtcaa tttttaatga
     2221 cgtgaattta cgataagcta ttattttgcg tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacagcga aatccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtctaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaacaaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat cgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaattg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tgttcacgaa
     3181 tataaagaaa tcatgagcga gatttcagaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttggg gtatatttct aggcaaagag aagaaaacga gcgagaaaag
     3301 gctctggtgg cttctgagag agcaaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatattgttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctacat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaaagcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttggtct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtctg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacctag cgttagcgaa agtttggggg cgcccttttc
     4021 aaagggagcg gttttatttt tgcatgacta agaatgagtg ggaaaaactt aaccgcattt
     4081 ctaacaggcg gttaaagtat caaagtttga cccttccctt aaaaatgcag tggtcaattg
     4141 accgaatttt atgcagaagt gggtttttga gttttggggt ttgaaacgag aaaaaagagg
     4201 tcgatttcga cctctttttt tgatgtttct tcttatcttg atactatagg caactatttc
     4261 tgcgaagaaa tcggggctat agtatgggtc tatcccttgt ggctctaagg aagaac
//
LOCUS       DAFELO010000125         4156 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFELO010000125
VERSION     DAFELO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.132"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            178..774
                     /locus_tag="LP452_002762"
     CDS             178..774
                     /locus_tag="LP452_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2015422.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="LP452_002763"
     CDS             898..2697
                     /locus_tag="LP452_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL2015423.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="LP452_002764"
     CDS             complement(2975..3187)
                     /locus_tag="LP452_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015424.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="LP452_002765"
     CDS             3649..3798
                     /locus_tag="LP452_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015425.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4051..>4156)
                     /locus_tag="LP452_002766"
     CDS             complement(4051..>4156)
                     /locus_tag="LP452_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2015426.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1493 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DAFELO010000126         4112 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFELO010000126
VERSION     DAFELO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.133"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(39..281)
                     /locus_tag="LP452_002767"
     CDS             complement(39..281)
                     /locus_tag="LP452_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2015427.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(313..870)
                     /locus_tag="LP452_002768"
     CDS             complement(313..870)
                     /locus_tag="LP452_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2015428.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(883..1071)
                     /locus_tag="LP452_002769"
     CDS             complement(883..1071)
                     /locus_tag="LP452_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL2015429.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="LP452_002770"
     CDS             complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="LP452_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL2015430.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2067..2240
                     /locus_tag="LP452_002771"
     CDS             2067..2240
                     /locus_tag="LP452_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015431.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2880..3353
                     /locus_tag="LP452_002772"
     CDS             2880..3353
                     /locus_tag="LP452_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL2015432.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3362..3589
                     /locus_tag="LP452_002773"
     CDS             3362..3589
                     /locus_tag="LP452_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015433.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1235 a          740 c          616 g         1521 t
ORIGIN      
        1 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
       61 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
      121 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
      181 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
      241 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
      301 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
      361 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
      421 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
      481 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
      541 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
      601 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
      661 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
      721 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
      781 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
      841 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
      901 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
      961 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
     1021 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
     1081 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
     1141 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
     1201 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
     1261 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
     1321 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
     1381 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
     1441 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
     1501 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
     1561 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
     1621 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
     1681 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
     1741 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
     1801 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
     1861 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
     1921 tttattaaat tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc
     1981 gttcttactt tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc
     2041 ttataaataa aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa
     2101 cgattggcta tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat
     2161 tcagttggac tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac
     2221 aaaattatcg aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta
     2281 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
     2341 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
     2401 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
     2461 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
     2521 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
     2581 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
     2641 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
     2701 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaata cactccacac
     2761 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
     2821 ttgttttaca ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa
     2881 tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt
     2941 tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt
     3001 ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg
     3061 cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa
     3121 gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg
     3181 gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg
     3241 taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa
     3301 aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga
     3361 catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga
     3421 attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc
     3481 aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg
     3541 tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga
     3601 acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt
     3661 acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat
     3721 gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca
     3781 actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca
     3841 ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt
     3901 tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat
     3961 tcttttttag taagctccca tatatactgt aaagccttct atatattatg tcatgttgct
     4021 ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct
     4081 tttttatttt taacattatt ttgataataa tg
//
LOCUS       DAFELO010000127         3894 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFELO010000127
VERSION     DAFELO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.134"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..321)
                     /locus_tag="LP452_002774"
     CDS             complement(<1..321)
                     /locus_tag="LP452_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2015434.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSART"
     gene            1063..1380
                     /locus_tag="LP452_002775"
     CDS             1063..1380
                     /locus_tag="LP452_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL2015435.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1471..1836)
                     /locus_tag="LP452_002776"
     CDS             complement(1471..1836)
                     /locus_tag="LP452_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015436.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2044..2526
                     /locus_tag="LP452_002777"
     CDS             2044..2526
                     /locus_tag="LP452_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015437.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2723..3613
                     /locus_tag="LP452_002778"
     CDS             2723..3613
                     /locus_tag="LP452_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL2015438.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1309 a          611 c          718 g         1256 t
ORIGIN      
        1 ggtacgagca gaacaactaa taggaaacgc tgcacgacca acaatcacat cgggtgtcca
       61 tccttgaaca acctttcttt gaatatattc tgattgttcc tcgggtaaaa caagcggacg
      121 tctaccacag tttgatttgt ttttcttata ctgttgataa tattctaagg ctgattttcc
      181 ttgcttgaag aacagatata ctttatggat cgtttgtctt gaacgattca agcaatgggc
      241 agttttagca acagattgat tcattttgaa ataagactct attattacaa gttcatccgt
      301 tgtaagatgg gtataggtca tttgtactca ctccttataa ttctttggtc ggaactattt
      361 tgagtgtagc acaaatgact tttttagttg tctagcttaa ttttacaatc ggcgacttat
      421 tgcttttgca aaatttattt acctgttttc tggatcaacg atagtgtaca ttatgctatc
      481 cttgtttgga ttttaggacg tttaaagagg cactttttta cataacttaa tccatagacc
      541 ttttaagcat gctgtgagat aataataaag aagagtttaa agcgcacccc aaaccacttc
      601 cccataagtg tattacgctt taaactcttt tatatttgaa ttcactaaga agcataccat
      661 atatttgaat ttactaagaa gcataccata tttttgaaaa aaagtgagaa aaaaggctta
      721 taattaggat ttagagtaat taattagatg tttattttat aggactgata ctatagatta
      781 caaatagtat cgtatttcgc aatcttaaaa ttatcttctt actgtgaata agataataaa
      841 atttgtattt gtccttttta ggtaacttat tttaatctat atacctttct ggatcaacga
      901 aagtatactt tatatagtca taacgccgat ggtaactatg cactgtaacg ataatgctgc
      961 taaaatatct ttttacgaaa aacatcacaa ggaaaaactt aactatggta ttatctaaac
     1021 ataatttttt cggtatttaa tatttaatta ggaggtaata ttttgctttt ttatagtaat
     1081 tttatcttaa tagtagcgat attattactt ctgaatatat ggatttttga taggagtaga
     1141 aattcaagta taggttttag aactaaacga agtttgtctt ctaaaaaaaa ttgggtatac
     1201 tcacaaacta ttttttatgg aggaattgtt ttaattagtt tactttcttc aacactttat
     1261 tctttgaata ttatagatgt ttccacctca aattcaatat ctattattgg aatcattatt
     1321 gctgcaatta ttacacaact gtttttagtg tttggagaga agaaaagaag taaaaaatag
     1381 aaaaactagt gatagttttg gtgatataag aaaaacatat atgtataaaa agaagaaagt
     1441 agcttctctt tgtacatata tggtttgttt ttatggatgc cgagcttttt tataagataa
     1501 ggctaccgcc cataacgacc caaggatttg tggaactaag taaatacctg caagaagcaa
     1561 gatcagctgg gtcggtttta aaggatgata gaagtaacgc cattcagtaa aattggcgca
     1621 aaataaatag gaagtgacag tagatataat attggcaatg aataaagata taatacgcct
     1681 gttcctttga aaataatatc cagcaaaaag agataaaaat atcaatgtga tgaacccgct
     1741 tacggaatga taacgaaaat caagcagtaa ggcgaaatgc acataaggaa caaaagataa
     1801 aagcaacgga aggtttttcc agcaagtttt tatcatcttt catcaccacc taacaagtgt
     1861 tattattttg ttagttttat tgtaaattga atcctgcaat aatcaattac tttttcatta
     1921 tttctagatt aaatgaataa actctcatgc cattatctct aaaaaaaaag tttttccccc
     1981 taattttttt agatagaaaa ttgtatagta tgatgagaga aaaagtagtt tgggggaacg
     2041 ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat ttttttggtt ttttcctatc
     2101 gttttgctac cttttacatg ggtaaatgct gttgtacaag gtgcggagat attaggcctg
     2161 atccaaggaa gcggatttgt ggtttctagg aattttccag ctttgattgt tttctattta
     2221 gtgataagtg taggtctctt attaccaaaa tctctgatca atcactacaa ttggcagctg
     2281 ctttactttt cttgcttatt agtatttaca tctatgtttt ctttgctgcc tcgcttgatt
     2341 atcggcccta atggtttgag cgagagtgga ttgaaggatt tagcttattc ctccatgtat
     2401 ttcttgacga tcaaaccatg gttctattta gtaattgcct catttattct tgcaacattt
     2461 ttttacttct ggacagaaat caggataaaa agtgaacgat ctgtatctat aaaaaataac
     2521 tattgatggt ggatcatcag taaaaaaaca tatgagctgc gtaaaatgca tgtttctcat
     2581 atgctttttt tattattatg taaagaatgc gaaaaacgtt tacatgattt ttacataact
     2641 taactatatt tttacagcaa ccatacgagg gatttacagt cgactgataa actttagttg
     2701 taaataagaa aggagcaata aaatgaaaaa agtattgttt actctaggta tgatgggaat
     2761 tcttttagcc ggatgcggct caggatcagg aaatacagat ggctctgcca cacaaaataa
     2821 ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta ggttcgacag cattacagcc
     2881 gttagtcgat gcagcacaag aaagctttgt acaagaaaat ccaaattatc aaatctctgt
     2941 acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa gctggagcag ttactatcgg
     3001 aaactctgat gtatttgcag aagaaagaga cggagttgat gcttcaaaat tagtggatca
     3061 taaagtagca gtggtcggga tggcaccaat cgtcaataaa gatacagatg tcaaagatat
     3121 caccaaacag gaattgattg atatctttac aggaaaaatc actaactgga aggaagttgg
     3181 cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac
     3241 attcgaaaaa tggggactgg atggtgctac ccctgtgcag tcccaagaac aagattcatc
     3301 aggaacagtt cggcagctgg taagtcaaac accaggagca atcagctatt tagctttctc
     3361 atatctcgat gattccacac aagcactaag tatcgacggt gtagaaccaa aagaagagaa
     3421 tgtagcagat aacagctggg gaatctggtc atatgaacat atgtacacga acggaaaacc
     3481 ttcccctgaa gtccaaaaat tcttagacta catgatgaca gaagaaatcc aagaaggacc
     3541 tgtcaaagaa ttgggctatt taccaatcac aatgatggaa gtagaacgtg atcacgaagg
     3601 aaatatcaaa taactagcag ataaacatgc cacggtagtc gttactatta aggaaaaatc
     3661 aactatgttt ccccacatat acatttttcc tttaattgtg aaaaatagtt cctcatattt
     3721 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat tcgaaaaaaa
     3781 gagctacgac atagaactgt cgtaactttt tttggctctt cgtcaaatcg tgttggtgaa
     3841 gacaaatttg aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg cata
//
LOCUS       DAFELO010000128         3576 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFELO010000128
VERSION     DAFELO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.135"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            116..976
                     /locus_tag="LP452_002779"
     CDS             116..976
                     /locus_tag="LP452_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBL2015439.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="LP452_002780"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="LP452_002780"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBL2015440.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="LP452_002781"
     CDS             1830..2678
                     /locus_tag="LP452_002781"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL2015441.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="LP452_002782"
     CDS             2688..3083
                     /locus_tag="LP452_002782"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL2015442.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3285..3566
                     /locus_tag="LP452_002783"
                     /pseudo
     CDS             3285..3566
                     /locus_tag="LP452_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1396 a          395 c          713 g         1072 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tatcaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc gctggtaagc
     3361 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     3421 agcaagcaca atcaacgggc tcattcaaaa aaagagacaa cttaacacca gaacaagcag
     3481 aattgatcgc actaagaaag aaaaataagc aactcgaaat ggagaatgat attttaaagc
     3541 aagcggcgct gatattcgga cgaaaagaca agtaat
//
LOCUS       DAFELO010000129         3536 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFELO010000129
VERSION     DAFELO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.136"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(184..741)
                     /locus_tag="LP452_002784"
     CDS             complement(184..741)
                     /locus_tag="LP452_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015443.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(920..1531)
                     /locus_tag="LP452_002785"
     CDS             complement(920..1531)
                     /locus_tag="LP452_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015444.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(1644..1901)
                     /locus_tag="LP452_002786"
     CDS             complement(1644..1901)
                     /locus_tag="LP452_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015445.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2498..2776)
                     /locus_tag="LP452_002787"
     CDS             complement(2498..2776)
                     /locus_tag="LP452_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015446.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(2810..3061)
                     /locus_tag="LP452_002788"
     CDS             complement(2810..3061)
                     /locus_tag="LP452_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015447.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1079 a          725 c          470 g         1262 t
ORIGIN      
        1 atttgccact ttgattcgta taaagctaac aactattaac tcctctcagc taatagttgc
       61 tctctttttt attcagattc atttttgctg atcacagcct agtatttcaa tggttttgaa
      121 cactaaacca aattgttcca ttacaacatt cactggctga tgaaatggat tagtagattt
      181 cgattattca aagcgatttt ctattggtgg atcgtgttcc caactaataa gacggattcc
      241 aaacctatcg tacagatctt ctaaaccact tataaatttt gcacctattt caggtgtttt
      301 attgcgtgga tattctattg ttacgccatt aaatggatac caattaggat taggctctct
      361 ttttttctgt acatctccca tagaatagtt agcactttct gggacataat atattcttcg
      421 accatcctct aattcatcta ataagatatt tcgtgatcgt ctatcacttg ctaaatgtag
      481 acgccacgtg cgataccatg ccatgctacc tgcatctgca gaaaatccat caaataatgc
      541 aacaattgta tcttctatca caatcctttc cccatctact tcatattcta atctaaaatc
      601 aaactcccct cgggtaattt ctggtcttgg tggcttagga cccaatatta ttctacttaa
      661 aaccgcattc gttcccagat gaaacacagc tataacaaca ataatgatta atattttgtt
      721 tctatttgat aaatttctca ttattcgcac atcacctcta cattctcaca tattagttcg
      781 taatactcct ttaatatcaa aatatctact ggtcgtaata atattcgcta tgaattccta
      841 ctactcgtat aagaaaggcc aaatcatcag cttttacttg ctgatgattt ggcttgtttc
      901 tatttatcgt aattgttttc taatcaccaa aattgttaac aatcggttca gatatttccc
      961 agttgattaa ggtaattccg tattgtgtca aaacttcttg cgcttctccg aaattaagta
     1021 cagttcttat gtatccactt tctcctaata taaagtctac ctctctttta aaaccttcct
     1081 cgagatttct aagggctata tgtggtttta atgacattga ttgtacattt tcccccatat
     1141 aatatcgcgc acttcctcga ctaaaataga ttcctagatc gtccccaaaa ggtagatctt
     1201 ctctaccact agctaatcta cttctccatc ttcgttctct tttcatagaa cctgcgctga
     1261 actcacaccc gttaaactca gctatgacag tatcttcaat tatgttaatc tgtccatcta
     1321 gttcgtattc aatcctaaat ggaaattctc cataggtgat ttccggttcg ggtattttag
     1381 gtgagattaa tgcaaagaaa ggaaatatta agcttggcaa catcaaaaaa actcctccta
     1441 tgatcacaat tcccgatgta atccaaaata taatttgtac aattctcatt gatccattgt
     1501 attccatttt attcccccta ttactattca tatccgccta tctatatcga tcttccaatg
     1561 cagagataat tgcttccata ccttgatttt ttactgagtt acctatgcga gatatcattc
     1621 tccactaagg tgcatcatat tgtctatcta acatgattaa ttctccaata ggtgtatact
     1681 cctccaaaaa caacatttaa tgcatctccc tcgacaacat aagctaccat gtttgctgta
     1741 tatgtgtttg agtctaattc ataagcagat aaatttactg tcgctggatt aaatataatc
     1801 gcattcgtat ttatagctac tgaatttgct gcttcagcac caccttttga atgtccgatc
     1861 attgtgattt caatagtagg atttacgtgg acagccctca ccgtttaccc tgaccattat
     1921 agaaatcgct tacttttttg aactacgcaa caaaggatac aatgctccta acttcactgt
     1981 ctccattttt gtccactaag gaatataaaa aataggcatt aattcattta attcggtcac
     2041 aacacgttac cttttaaatc attatactgt atctcataca ttattatcaa taaactatta
     2101 taaaaatgca aaaaatgctt tatcaatcta ttttgtaatt ggtcataata tcattgaaat
     2161 cataccctgt atcgattttt cctatgaatc tcgtctattt gtgattgacg aaaaagaggc
     2221 aagacagaca ctggaatggt aattttaccc actattttgc tccaactatt tgtaatcgta
     2281 cccacttttg aaataccgag atgatcattt taattgatat gctgattccc aagcaaaacc
     2341 ccaagaaatt aaatgctcta tcgcatctag cttcttgggt tttgcttata taaattccaa
     2401 catttaaatt ttttatgctg ttcggtctca gaaatgccgt ttttgcttat atcacaatat
     2461 caagatgtca aattattcgt gttgccgatg actttgatta atttttttca aattgtttat
     2521 taagactaat gctgtctttt tgcagatacc aatgttcgaa ctttttaaca ttatcatatg
     2581 atcctgcaag ttttaacttc ttaccattag atgttatcaa agttataatt aaccttgcct
     2641 ttttcccatt aggatctttt attttatgaa ttttctgcag gtaatctctc ctaatggcag
     2701 ctatttttac ctcatcagtg ctgataaacc attgatcatc cacatacata ctgcctgtta
     2761 tgttgtgttt ttccatttct tccgagaacg aaaagtttaa ggctcgttct tacttccaaa
     2821 gttgaatgag tccatggatt ggacttagtg tgagaattga gaacaacaat aagaatccat
     2881 atcctcccgc aaacatccct aaggctatac tgtctctcca tccataagta ggattagaag
     2941 agaaaagagt caccgtttgt aataatatga aaaggaataa tccagcacaa acaccaaaga
     3001 taacaactcc aaacattgaa tttttataca atgaccattt atttataggc ttttctttca
     3061 ttttacttcc ccctactgtt ctgttcacac ggaatgaatt caaagttcat aagccctcct
     3121 ctttgaacat atgaatacga cttgctaact atactttaaa aaagcttgtt ttaagcggtt
     3181 ataggcgagt tgtaatctct attcatctca ttagcaaata acttgccgaa aagtctactg
     3241 aaattaaagc agatagtgga agaattattt agccaggata gacactattt gtgaaacaca
     3301 tgtctttact tttgaaattc tatgatgaaa agccttggaa tacagtagct cttaattggt
     3361 aatacttttt ccattataaa taacctcttt tcattttata tttatcccct ctaaaaataa
     3421 tatctaatct aatattttaa acttatattt ccctcgctgt gctaatagag cattactaaa
     3481 taaatagaaa ctagaaaaaa gcattacgcc tgccagaata aaccaattat ttgtat
//
LOCUS       DAFELO010000130         3481 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFELO010000130
VERSION     DAFELO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.137"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(354..1439)
                     /locus_tag="LP452_002789"
     CDS             complement(354..1439)
                     /locus_tag="LP452_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2015448.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1759..2166)
                     /locus_tag="LP452_002790"
     CDS             complement(1759..2166)
                     /locus_tag="LP452_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015449.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(2185..3003)
                     /locus_tag="LP452_002791"
     CDS             complement(2185..3003)
                     /locus_tag="LP452_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2015450.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(3229..>3481)
                     /locus_tag="LP452_002792"
     CDS             complement(3229..>3481)
                     /locus_tag="LP452_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL2015451.1"
                     /translation="YIIKNLGITVEQFCELYAYPQGTVASWITRQRRIKSLPASFVYD
                     LSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT         1061 a          539 c          477 g         1404 t
ORIGIN      
        1 aacaactaaa tttatacaaa aaagtgtaat tgaatattag cagcactgcc tgaaaagaag
       61 taatatcagg cttttcagct tgggtaaata agtgaattac ctctcttcat gaattattat
      121 aactcaaaaa aatacatatg caaacactag ttccctgttt ttttgaatga aaaataacta
      181 aaatatctaa cgattatgac attaaaacct acgtttgtga ttgttttcat aaacttttaa
      241 tctaaaaact ccggaataat aactatttat ttgtaacatt tgtaataaaa ttaaaattgt
      301 aaaaaagttc taatttaaaa ttgatcattt ttatagtgtt cgctttttat caactactct
      361 agccaatttt ccgtactaac tgttggtaat tcgttaaatg tttctctttg ttcgtttgca
      421 agctttgccg caatagcttt ttcaataaac cagtttttta catacacaaa aatcaaattc
      481 tgcaaattat ttactttctc agtcttttgt ttttgataag ctttcagcaa cgtcatatat
      541 aatcctttat ccgcatcaat tccgtaagat tcaagttctt catacacaac taccgtcttc
      601 tcaagttcct cggctttatg ttttgctttg tgtattgtgg tgacggtttg ttgggcttct
      661 tcgtaagtgt tagaaaatgt ttttatcaag cttaatacct tttctggtac aaatgtgttt
      721 atgctgtctt ctttcatgat ttcaacgaag ttatctagca aaacttgatt ttgtaattgg
      781 tctgtttcag tgtcttttaa gtgtctttta gtgtctaaag atttattaat ataataatct
      841 tgtgcgattt ccgcatttcc agtgttatca agggtttcag ctgttttttc tcgacgtgcg
      901 atttccgcat ttccagtgtt atcaagggtt ttagcagttt cctctcgacg tgcgatttcc
      961 gcattccgag aagaggttga tttatcaata gtttcgctta aatcgtcatc gaaagaattt
     1021 tgaataaaaa gatcatcggc acttatttca ggttgaagta aatagtattt atttttttca
     1081 ttttttttcg tttttgggtt aaattctcct tttacgactg aaatcaaatt atatgtttct
     1141 aactcgttta aaatggtttt tactttgcct tggtagcaat ttaatttgtc cattaggtct
     1201 gcttgtgtaa attccaaata taatagacca tcatcgtcga tccatttttc atgtaaggct
     1261 ctacgaaatt catttttaaa aagcatatag gctatttttg agctgtcttt cattttagag
     1321 tatttagaat tctgaattaa aaaactagga aatttataat atttttcaat ataaacattc
     1381 gttgctttat atccccaatt tttaattatc attttttgag attgattttc tgtagacatt
     1441 aaatttctcc ttcttcttag aaacttgtat atattcagga ttttcaacaa gtgaactata
     1501 ccatgtatta agggtgaata aaacactttc agtatttaag gtataagtta tatattaaaa
     1561 aaataagtat aagttatact ttaaatacaa ataatgtata acttatactt taaataataa
     1621 ttttataatg ttgatttaat cgcaaattta cacgttgttt attaagttat accttaaata
     1681 tttaatgtat aacttatact ttaatattca tttatctagt ataacttata ctttaattga
     1741 ataccgcatg aaaaaaaatt attttggtaa tttttcaaac tcttcttgta tttcttctct
     1801 tatgtgttct tcttttgcta ctcctagtct atgctggata tatgagtctg ctaatgtatt
     1861 aacaattttt tcgaaagaaa ttctcccttt ttcactttgt aactccgtca tatagtcttg
     1921 taaaattgag agtttgctat ttaatgccgg agagatcttt ttaggaactg ataccttgtt
     1981 tcttttatca ggaattgaaa ctggtcggcc tctttttttc aatgtaatat ttcttgtgtt
     2041 actcaatgca ctattgctaa agttatattc accgcttatt ttcacatctt ggcgtttgtt
     2101 attgggtaat aatggctcaa attcaccatt ctccatcatt ttaggaatat caaaatcttt
     2161 aggcattact tatgcaactc cttattattt atttaaaaac tcttgtctag catctagttc
     2221 tgccaaaata tttataaata cttggtgtgc tttataatcc cattgatcaa aatttccatt
     2281 tttgtatttt cttaaatgta taccaatttt agaatacctt ctcaatcttt tttgataatg
     2341 aatgagattt ttggatacca cggttccgta tttttcaatt gcttcttcta ggatttcttc
     2401 atcaaaatca tcatttggtt ctaccataca aggtacaata tctataattt gcaaatcaag
     2461 atcgtatcta tcgaccatga atttttgata atttacatat ttgttgactc cctctaagga
     2521 ttcttcagct gtttgaaatg aaattatcga atagtctgaa gctgccatag cattatcgga
     2581 aaattcgcta atggttggtg gtacatcaat aaatatttct tgataattgt tctttattgg
     2641 aagaagtaat tttttcaaga cagtaacttg gtctaattca ttttcatgtt tattagcaaa
     2701 agcggagaat gatcgaaaac tagtgtcaca tgccagaagg tcaagatttt catttacctt
     2761 aataatacta cttgctaatt ctccatttgc gacaccattt gttatagtgg ttttgatatc
     2821 ggttataccg aaagttcttg ctatgacttg tgttaagttc gcttgagggt ctaaatcaat
     2881 aataagcgtt tttctatttc ttatcattgc attgtcataa gcaagcatgc tgactatctt
     2941 agttttacct actcctcctt taaaattgcc aactgtaaat gtagtccctt gaaaagtatc
     3001 catgtaaatc ctcccaatct ttaattgata attaaagtat aacttatata tcggaaatgg
     3061 tcaacattaa aagtataact taaataataa tatctaaaaa cgtttggata tagcggtttt
     3121 taataataaa agtataagtt ataccatata tatttatggt ataacttata cttttattat
     3181 taaaggtata acttatacct taaatattgc ttttcgaaaa tagctattct agtctatctg
     3241 tttctttact tttcgctgct gatttgaatt gaattgatca tattcatttt ctagtattaa
     3301 taacttttcg tatacatcgg tcatattcat actagaagca agacttagat catatacaaa
     3361 tgaagcaggt aatgatttta ttctacgttg tcgggttatc cagcttgcaa ccgttccttg
     3421 tgggtaggca tataactcac aaaattgttc tacagtgatt cctaaattct tgataatgta
     3481 a
//
LOCUS       DAFELO010000131         3277 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFELO010000131
VERSION     DAFELO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.138"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(552..2165)
                     /locus_tag="LP452_002793"
     CDS             complement(552..2165)
                     /locus_tag="LP452_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2015452.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="LP452_002794"
     CDS             complement(2306..3214)
                     /locus_tag="LP452_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015453.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1061 a          560 c          573 g         1083 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaat
//
LOCUS       DAFELO010000132         3199 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFELO010000132
VERSION     DAFELO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.139"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            301..585
                     /locus_tag="LP452_002795"
     CDS             301..585
                     /locus_tag="LP452_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015454.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="LP452_002796"
     CDS             588..1004
                     /locus_tag="LP452_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015455.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="LP452_002797"
     CDS             complement(1141..1860)
                     /locus_tag="LP452_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL2015456.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="LP452_002798"
     CDS             2513..2608
                     /locus_tag="LP452_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL2015457.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="LP452_002799"
     CDS             complement(2910..3155)
                     /locus_tag="LP452_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015458.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAFELO010000133         3070 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFELO010000133
VERSION     DAFELO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.140"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(334..696)
                     /locus_tag="LP452_002800"
     CDS             complement(334..696)
                     /locus_tag="LP452_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015459.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="LP452_002801"
     CDS             complement(770..1150)
                     /locus_tag="LP452_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL2015460.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="LP452_002802"
     CDS             1214..2407
                     /locus_tag="LP452_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2015461.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="LP452_002803"
     CDS             complement(2484..3068)
                     /locus_tag="LP452_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2015462.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          504 g         1021 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat
//
LOCUS       DAFELO010000134         2993 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFELO010000134
VERSION     DAFELO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.141"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(23..1189)
                     /locus_tag="LP452_002804"
     CDS             complement(23..1189)
                     /locus_tag="LP452_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2015463.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1201..1554)
                     /locus_tag="LP452_002805"
     CDS             complement(1201..1554)
                     /locus_tag="LP452_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL2015464.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1682..2704
                     /locus_tag="LP452_002806"
     CDS             1682..2704
                     /locus_tag="LP452_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL2015465.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            2723..2884
                     /locus_tag="LP452_002807"
     CDS             2723..2884
                     /locus_tag="LP452_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015466.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
BASE COUNT          973 a          610 c          550 g          860 t
ORIGIN      
        1 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
       61 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
      121 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
      181 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
      241 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
      301 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
      361 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
      421 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
      481 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
      541 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
      601 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
      661 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
      721 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
      781 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
      841 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
      901 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
      961 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     1021 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     1081 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     1141 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     1201 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     1261 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     1321 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     1381 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     1441 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     1501 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     1561 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     1621 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     1681 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     1741 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     1801 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     1861 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     1921 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     1981 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     2041 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     2101 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     2161 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     2221 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     2281 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     2341 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     2401 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     2461 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     2521 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     2581 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     2641 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     2701 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     2761 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     2821 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     2881 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     2941 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaa
//
LOCUS       DAFELO010000135         2666 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFELO010000135
VERSION     DAFELO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.142"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..318
                     /locus_tag="LP452_002808"
     CDS             <1..318
                     /locus_tag="LP452_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2015467.1"
                     /translation="KAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIG
                     KRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHL
                     VKK"
     gene            732..1550
                     /locus_tag="LP452_002809"
     CDS             732..1550
                     /locus_tag="LP452_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2015468.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1623..1982)
                     /locus_tag="LP452_002810"
     CDS             complement(1623..1982)
                     /locus_tag="LP452_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2015469.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          924 a          371 c          471 g          900 t
ORIGIN      
        1 aaagcaatca ttcctgagaa cttagatgag gttaaacttt atttatggga agataacgga
       61 gaaattgtcg gttttagtgg tgtaagtgca gaagaattag ttatgctatt tcttgatcca
      121 gattttattg gtaagcgata tggtagtcaa atattaattg agctgattga aactgaagat
      181 ataaaaagaa ttgatgttaa tagtcaaaat aaatatgcaa aacattttta tttaagccat
      241 ggatttgaaa ttgaatctga agaagactta gatggttttg gtaaaccata ccctataacg
      301 catttagtaa aaaaataatt catgaatggt ttattacctg attgaacatg aataaaagta
      361 atatttttat tttgatggcc tatttctagg ttatgaatta tccagatcaa atttgtgtat
      421 tacattcagt aataaggtgc ttatcttagg atcaacttat gatactagct gaccaaggat
      481 gtaccagtta tagaaaatca gttaaagtaa aaatgataaa aatcttatgt caacactgat
      541 ttaggctgac ctgtgggtta ggagaaagac aggtcggtag cttttaatac aaaaaatggc
      601 ttgtatgtat ctagatagtt aactgaaatt agaaatgttc ttatttttaa cggagtaaga
      661 aaaataggct gctttaggtc tgaatacttt ttcaatataa tgaagatatc aaaggcgaca
      721 ggagtgtttt tatgaataag aatcctcata atctaaagtt ggtagcagta gatatggatg
      781 gaacatttgc tcgtactgat tacacatacg atatcccaag gttcaaggct gttttatcgc
      841 gaatgaaagc tgttgattgt caatttgttg ttgcaagtgg aaatcaatac taccaattga
      901 gatcgttatt tccaaattat tatgatgaat taagttttgt tgctgaaaat ggtgcttttg
      961 ttaaagataa aacagaggta ctattttcag ccgatatgcc tagaaaagcg atcgaggtgg
     1021 tccttgattt atgtaaagac tatcctgaag taaaaaatgt gatgtgtggt ctgaatagtg
     1081 cctactgtga aagaggaaag gtgagtcaag cattttttga tctaaccaat atctattacc
     1141 atagattaga atgggtagct gattttgcag aagttcaaga taaaatttta aaatttgctc
     1201 cgactatgcc tgttgaaaaa accgaatttt acgttgaatt gttccgtgaa aaattagccg
     1261 ggctattagt acctaccagt agtggtcatg gttctgtaga cttgattctt tctgagtgcc
     1321 ataaaacttt cggcttgaaa atgttagttg agcgcttggg gattaatcct gaccagtgcg
     1381 ttgcttttgg agatgggggc aatgacattg agatgttgga gtattgtggt ctgagttatg
     1441 aaatggataa tgcaacggaa gctgttaagc aagttgctaa gcatcaatgt ccttcaaatg
     1501 acgaagacgg tgttttagtg acgctcgatc gattgttccc taacacgtaa acactaggta
     1561 attcatcaaa aaacctcgat ataagaaata ttcgaggttt taaaactata tttagaacag
     1621 atttacgaag cgtctgatga gtcaaaatag acttggacat tgctatctaa aataccactt
     1681 tcattcttta aacgaattgt ttgatttgat ggagttacct ctaatactaa atgcccatta
     1741 ataaaggtag tatcgtattc agatgtttta actgttgctt cttcgcaagg tattgttaaa
     1801 tcatatgctg gaatataatg ttgtgtggaa actaataaaa aatgatcacc aatcatacag
     1861 catccctctg ctgaagaaaa ttgattagag ccatcattta aagttaacaa gaatccatcc
     1921 tttaaacgag gtacttgctt aaatacttct ggttgtactt ctaaaaataa attatcgctc
     1981 attttcacga ccaccatcga cttccacagt tgaaattgac attttattta acacacctat
     2041 tcccccttaa ctataactca attctatata aaaaatattc tattctcaaa taattgaact
     2101 tttcaaatcg ccaatttact tcggctttat cagagctcat tcatatcaag agcgattaac
     2161 gctgtaaaaa ttaaaccgaa gatctgataa attttctcta ttttctacaa tatttaaaac
     2221 gcttccttag tctattatat atctatttct cttaggagta tgtttagatg tttgaataat
     2281 agttgcccaa ctttaaaggt tattcttata atattttact tcattttcca atagaaaatt
     2341 caaaggatta ctggtttttc taaaatagtt tagtttaaat acataagtat cctccgacac
     2401 tgtattagtt cataatcatc taaatacaaa attttcaaat aaaaatattt caagctctat
     2461 cgttgacgaa tgtaaacgat acttataaag taatagaaat tgattacatt tgtaaactta
     2521 aaatggttaa tttatctcac ataaaactaa gaataaaata atgattaaaa gtggttagtt
     2581 tttatacgat ttagcctatc ggtaaaaatg aattagagag tgtaaaatat tttgtgtaaa
     2641 tgaaaaaatc catacaaaaa aggaag
//
LOCUS       DAFELO010000136         2601 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFELO010000136
VERSION     DAFELO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.143"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            5..1150
                     /locus_tag="LP452_002811"
     CDS             5..1150
                     /locus_tag="LP452_002811"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL2015470.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="LP452_002812"
     CDS             1321..1779
                     /locus_tag="LP452_002812"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL2015471.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="LP452_002813"
     CDS             1938..2408
                     /locus_tag="LP452_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL2015472.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="LP452_002814"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="LP452_002814"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          852 a          499 c          491 g          759 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt a
//
LOCUS       DAFELO010000137         2468 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFELO010000137
VERSION     DAFELO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.144"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(493..1785)
                     /locus_tag="LP452_002815"
     CDS             complement(493..1785)
                     /locus_tag="LP452_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2015473.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSVGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="LP452_002816"
     CDS             complement(2059..2319)
                     /locus_tag="LP452_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015474.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          824 a          458 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accaactgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DAFELO010000138         2398 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFELO010000138
VERSION     DAFELO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.145"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            207..2069
                     /locus_tag="LP452_002817"
     CDS             207..2069
                     /locus_tag="LP452_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2015475.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="LP452_002818"
     CDS             2069..>2398
                     /locus_tag="LP452_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2015476.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAFELO010000139         2289 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFELO010000139
VERSION     DAFELO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.146"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            1173..1727
                     /locus_tag="LP452_002819"
     CDS             1173..1727
                     /locus_tag="LP452_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2015477.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1981..>2289
                     /locus_tag="LP452_002820"
     CDS             1981..>2289
                     /locus_tag="LP452_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015478.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          887 a          253 c          355 g          794 t
ORIGIN      
        1 ggaaaacgta gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa
       61 tttatataaa ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata
      121 atttttaggt ctatttaact catcagacat tctatgctca ttttctccaa atttgtttca
      181 actctttcgt acttaatttt gcgtatttga aaattcttct tttagagtac ttttattacg
      241 atactagtat gatatatatc tatagcaaac agttaaaatc ttcctaaaat aagcgaatag
      301 cattcctaaa acctataaaa tttttagatg aagcgacaag tggacaatta ataaactact
      361 tgaacatgaa attatggatc acctttcaaa tttagaaaat ggaattctta tacttattgc
      421 gcaacattta ttgattgcaa aattatgtga caaaaagaac tagtagaaga aggaataatg
      481 gcttgataat aaagtactac ctattaaagg tagtacttta tttaatttag tataaattaa
      541 catactacta tattttaagt ttcatattat ataaattaat aatatactac cattttttgg
      601 tagtataatt taaaggtaat aagttattct tttattacta tgtttgggtt ttatagcaag
      661 gaaagtttaa taagagtttg gacctagcaa acaatattaa tgaggtcatt aaatcttatt
      721 atctttagtg atataacgaa atatctacac agacaaaatt atgatcttgc gtaaagttat
      781 aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta ttcatatgct
      841 aatttatttc actttatagt aaggggagtt cttagtatat tgtccaaatt taaatttcat
      901 ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg cccgtctaaa
      961 atttaattaa gagcttagaa tcttcatata agtaaggtta atttttttgt acaaaatagt
     1021 cggaatattc tgagttactg aaatgatata aaatcataaa ctaaacccct tgattttctt
     1081 cacttttaac agagtatata tataagttca tgattatggt aaaatatata tataatcatg
     1141 aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga
     1201 caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat ccagaaagag
     1261 atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat
     1321 taaaagttgg agacatacga ggtaaaagta agcctattat tgttgaacat aaaacaggta
     1381 agcgaaaacc gatttttata gataatttga gagaagagat tttattatat actgaaggta
     1441 aagaagaaaa tgattggtta tttccaagtc ggcaacaagg tcgacatata acaagggatc
     1501 gtgtctatca gatatatgca gatatagcag aaaaattagg aagagatgat ataggaacac
     1561 atacattaag aaaaactttt gggtatcact actacaagaa aactagagac attgctacct
     1621 taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg
     1681 aagatgaaat aggggcatcg ttacgtggtt ttaaattggg aggctaattg agatattagg
     1741 atatattttt tgtgtataat gaaaacttag aaggtgagat taatgaagat aactgaattt
     1801 tcggaagtat ttaaaacgtt tagtaacatt tacaatagaa taccagagat agaacaaaat
     1861 gttgtagttg ataatgatta ttatatagag aatgatttta ataaaccaag aaaatatgac
     1921 aacctataca attttataca caatagaaca acaataaata acaacagtaa cactcgagtt
     1981 ttgattaatt cagcatattt tcatattaaa tatttaaaga aatatgagta tgaagaagtt
     2041 gtattagatt atgggctaaa tagacagtca tttttaattt atgcaataaa taacggaaat
     2101 ttgccaagca ataaaaagaa cataattatt gaaacatatg cctatctgga ggggggatct
     2161 aaaaaactaa ttaagtcatg taatatagag tatcaattat cagtaggaga aattaaatgt
     2221 gttttcgaag aagatttaga acttgactta aaaaaaattt ttgccagcat tcatgattat
     2281 ataggtttg
//
LOCUS       DAFELO010000140         2260 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFELO010000140
VERSION     DAFELO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.147"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..1444)
                     /locus_tag="LP452_002821"
     CDS             complement(<1..1444)
                     /locus_tag="LP452_002821"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL2015479.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
     gene            complement(1782..>2260)
                     /locus_tag="LP452_002822"
     CDS             complement(1782..>2260)
                     /locus_tag="LP452_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HBL2015480.1"
                     /translation="KALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVL
                     IIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFV
                     SKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
BASE COUNT          659 a          422 c          387 g          792 t
ORIGIN      
        1 cttcttcttc aaaggtatcc acgtaacggg gaatgtttaa gttgtaatca ttctccacaa
       61 tttcatcaaa ttctgcgaca taagcatact tgtctttaat tgtacgggtt ttataggcct
      121 ctaaaatagc gtcaatatgg taatctcgta gcgtattttg cgtttttatt ttttcaaaat
      181 cttgtgacgc atcaataaag agcacatctt ttttcgcacg gtctttcttc aagataatta
      241 cggtcgttgg aatggacgta ttaaagaaga tgttagcagg taagccaatg acggtgtcaa
      301 tggcaccatt ttctaacaag gctttgcgta tcttcccttc tgcgccccct ctaaagagca
      361 ccccatgtgg caaaacaatg gccatgacgc ccgtatcttt caaatgatag tagccatgta
      421 ataagaaagc aaagtccgct tttgatttag gggctaatac gccataggca gagaagcgtg
      481 ggtcatctaa gaagccttta tcagccgacc attttgcaga atacggtggg ttcatgagca
      541 ccccatcgaa gttcgttggc tcttctgttg gccagtcttg atccaaggta tccgcattat
      601 gcaagtgttg gttggcaatg ggaaccccat gcaaaatcat gttcatacgc gccaaattat
      661 aggtagacgt attcaactct tgtccgaaat aagaaatggt tcccggttga tgggaatatt
      721 ttttagcgtt caaaagtaac gagcccgaac ccatggtcgc gtcgtaaaca gaaaagcctt
      781 ttttatcttc ttttccttgt aaaacaattt gtgtcatcaa ttttgcgacc ggttgtgggg
      841 tatagaattc accggctttt ttaccggatt cagatgcgaa ttggccaatt aagtactcat
      901 aggcatcgcc taagacatcg ccagcatgcc ccgccatgtt taacccttct aattctttca
      961 taacatctgc gatggtttta ttttgttttt gtggggttga ccccagtttt ttagaataca
     1021 gatcaatgtc ttcaaataag ttctcaaagt tttcgctgga ttgttcaatg tcacggaaac
     1081 cttgtaccag gtcttctaat tgaaaagatc catcatgaat ggctgccact tgcgcggtaa
     1141 aggtcaatgc gggtgcgatg gtatatgaaa actcatcttt tagttcatac aagagatcat
     1201 cctttatatc tgcatcttta aatgcttcaa cgtaaaggtc ttgtgcttta tttaggtcgt
     1261 ccgtttcttc ctccaataga acggagacat accgcagcat gttgtcggat aagtatttat
     1321 aaaagaccaa gcctagtaaa tagctcttgt attcattagc gtccattttt gaacgcagga
     1381 tatctgcgga attccacagt gcctgatata agctttttga atttttttcg atttgttctg
     1441 tcattggtta ttttcctcat tcgtgttgta ttttttataa atcagttttt taaaaaatta
     1501 gttactattt gaacttgttt tttcaccata tgtattttaa tgaaatggat aagctacatt
     1561 tgcttagaca attcatgtct tacttaattc ttattataac aaacctattt cacctttttc
     1621 gatacattaa ataataagta acttaaaata aggttcgaat aaccttgtta aatagtggtt
     1681 ttcttatctt atagtataga aatcaagaga ttacttccga tataacattt ttttaaaaaa
     1741 attagattgt aatgtttttt atattcattc taaattttca cttaaaaagg ataatcatta
     1801 aaatcatcaa attcagtaat atatattttc ttactcattt taatatattt ctcttcgtct
     1861 ttaatggtcc cttcataaaa agatttctta gcatcttcat ttagccagtc tacagaaaac
     1921 atctgattag agacaggatc tttgatttta gatacaaatt cggatgataa aacactatca
     1981 aacatttctc caaaaatggt ggtcaatctt tgaaaccata tcatcataaa ttcagtattt
     2041 ggctttaaag catatttgtt tataatcatt tcaactattt ctctagcctt actctcattt
     2101 tcgaaattta ataactgccc tataattaag acacactgcg gcaaagaacc cgggttattt
     2161 atcatgatat ctaccaggat accaataagt ggtaaaagat cgttcggctt tgaagtgatt
     2221 ttaataatgc gttctttatt aaactcagaa agtgcttttt
//
LOCUS       DAFELO010000141         2257 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFELO010000141
VERSION     DAFELO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.148"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..308)
                     /locus_tag="LP452_002823"
     CDS             complement(<1..308)
                     /locus_tag="LP452_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2015481.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     L"
     gene            982..1794
                     /locus_tag="LP452_002824"
     CDS             982..1794
                     /locus_tag="LP452_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015482.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          745 a          326 c          377 g          809 t
ORIGIN      
        1 agggcttttt cgtttcgttg ataacgttca aacactgtgg gtgaaaaatg gccatcacgt
       61 gttctgggta cttttaattc tagcgtgcct atacgtgtcg taaagctgcg ctcataatag
      121 ccatttcgtt gactttgtcg gttttctgtt cgttcatatt cttttgcttg aatatattct
      181 gttcgttgat tttccattag ttgattaaat accgttgtta aaatattttt agaaacgtca
      241 tcctttacag aatattcaat aatgctttga atctcttcgc ttttcagtgt aaaatgtact
      301 tgggtcatgt aaaagtcctc ctgggtatgt ttttgtcgtt aaaaacattg taccgtaaaa
      361 ggactgttac atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca
      421 gttatttgtt aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata
      481 aaaggttttt gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag
      541 tctttattct tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc
      601 ttttcacttt tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac
      661 cacatttaat agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag
      721 ccgtaatatt ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt
      781 gggatactga atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc
      841 agccttctta ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca
      901 atttcagaat caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt
      961 ctttatgaaa gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat
     1021 acccatattc tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct
     1081 cttttctcta agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa
     1141 gctgttggtt ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc
     1201 ctacatcaaa aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt
     1261 tctgatagtt taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt
     1321 ggtgtggttt catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag
     1381 attattaatt tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa
     1441 tttttacaaa aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa
     1501 gtgctttttg gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa
     1561 aaaagtatgt gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca
     1621 gaagcgaatt attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa
     1681 gatgttacac tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat
     1741 gcattgaaat ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag
     1801 aatgcatctc ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag
     1861 caaaatatca cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa
     1921 agagcactat agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta
     1981 tagttgaggt ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa
     2041 taacttttat attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag
     2101 attagataga tattataaat atgttgattc tattttcata gcaatggaga aaatacatta
     2161 gcaatatata aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa
     2221 caaatataaa atttaataaa aatatagaac taaatag
//
LOCUS       DAFELO010000142         2214 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFELO010000142
VERSION     DAFELO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.149"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            4..>2214
                     /locus_tag="LP452_002825"
     CDS             4..>2214
                     /locus_tag="LP452_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015483.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVIL"
BASE COUNT          883 a          358 c          496 g          477 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat ttta
//
LOCUS       DAFELO010000143         2201 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFELO010000143
VERSION     DAFELO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.150"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..738
                     /locus_tag="LP452_002826"
     CDS             <1..738
                     /locus_tag="LP452_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBL2015484.1"
                     /translation="KDKQIVCGNCMKKAGYGMTTPYKIIRSLNLEDLDKDPSEKRKLN
                     ISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKKWTTHHFNLISDQLNSDEYV
                     IFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGNNVQSIILDNLNNISKKRGV
                     LLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKRRNISATNSNTSTISSASEI
                     RKYKELLDDGIITEEEFNKKKQELLDL"
     gene            834..1136
                     /locus_tag="LP452_002827"
     CDS             834..1136
                     /locus_tag="LP452_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015485.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1650..1723)
                     /locus_tag="LP452_002828"
     tRNA            complement(1650..1723)
                     /locus_tag="LP452_002828"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1687..1689),aa:Ile,seq:cat)
     gene            complement(1913..>2201)
                     /locus_tag="LP452_002829"
     CDS             complement(1913..>2201)
                     /locus_tag="LP452_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2015486.1"
                     /translation="GWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSG
                     NVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          795 a          332 c          359 g          715 t
ORIGIN      
        1 aaagataaac aaattgtttg cggcaattgt atgaaaaaag ctggttacgg tatgactact
       61 ccttataaaa taataagatc tttgaattta gaagatttag ataaggatcc atcagaaaaa
      121 agaaaattaa acatatctga tcatactgca caaaacaatc atgatatagg taatgataga
      181 atgagaacca ctgaagagat ggtagaattt tgtcttaaat acggctacgg taaaggaatg
      241 actaaaaaat ggacaactca tcattttaac ttaatatcag atcaacttaa cagtgacgaa
      301 tacgtaatct tctgctttgt aggattgcat aattatatct catcaactaa gcatgacaat
      361 aattatgcat acgcattaac taataaacgt ttaattgtag ctcaacaaaa aatgataggc
      421 aataatgtac aaagtattat attagacaat ttaaataata ttagtaaaaa aagaggagtg
      481 ctgttgggta cattaactat tgatactctt ggtaaagtat tgaatgttgc agttgataaa
      541 gatactgttg ataggataag tgattcttta aacgaaatta tttataattt aaaaagaaga
      601 aatatttctg ctacaaattc caatacatca acaatttcct cagcttcaga aataagaaaa
      661 tataaagaac tattggatga tggaatcatt acagaagaag aatttaataa aaagaaacaa
      721 gaattattag atttataata tctatcaaag aagatgggca tttaatttag ctcatctttt
      781 tctatttaca atacaaacat ttgttcgtat actctttttg aggtgaactt tatatgttaa
      841 tggaaggaaa aacacaatta tggtttaaat tcgatccttc gaatagattt gtcaaagatt
      901 tttataaggt gtgggattca gaagttttct ttttagcaat cgaagatagc ttattaatca
      961 atctctacta ttctaataag aactatttca aaattcctgc tgcgaaaact agaatgaaga
     1021 agggtgtata ctttttgttt gatatcgtga ctgacgtgcc ggacgcacgt agcgatcatc
     1081 ggcgttatga ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta
     1141 ggctacctaa aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt
     1201 ttttaagtta gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc
     1261 tataaaatca aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca
     1321 ctttttttca aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg
     1381 taacacactt atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc
     1441 acaatttaac ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag
     1501 aagtataact atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac
     1561 tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta
     1621 acacagaacg attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg
     1681 acggttatga gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa
     1741 agccataaac aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga
     1801 gtgtgactat ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaataa
     1861 tttttatagt tgtcaatata gttatgtact gctcctcaac gaggagctat ttttatcgtt
     1921 taggaatatt taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc
     1981 cctctggatc attcgttgcc cgcatcatta catagacttt cttactagga aaattacgca
     2041 tattgaaaga tacatgataa ccaacatttc cagaagtatt ataagcttgg tttacatctg
     2101 gtctataaat tccatcagct cttactcgag ctaattcttt cccagtattg tagtccataa
     2161 tgaaaatata ctcgtattta tagttagcaa tgtgccatcc a
//
LOCUS       DAFELO010000144         2173 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFELO010000144
VERSION     DAFELO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.151"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            227..844
                     /locus_tag="LP452_002830"
     CDS             227..844
                     /locus_tag="LP452_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015487.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            885..1184
                     /locus_tag="LP452_002831"
     CDS             885..1184
                     /locus_tag="LP452_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL2015488.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1218..1385
                     /locus_tag="LP452_002832"
     CDS             1218..1385
                     /locus_tag="LP452_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2015489.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1430..1993)
                     /locus_tag="LP452_002833"
     CDS             complement(1430..1993)
                     /locus_tag="LP452_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015490.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2021..>2173)
                     /locus_tag="LP452_002834"
     CDS             complement(2021..>2173)
                     /locus_tag="LP452_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2015491.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
BASE COUNT          691 a          370 c          422 g          690 t
ORIGIN      
        1 tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga ataaatataa
       61 tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg ccttgatttt
      121 ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc tgatttaatc
      181 tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga gaaaattttt
      241 ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt ccgcgaagga
      301 attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag attttaaacg
      361 ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct atctacaatc
      421 attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag aattaactaa
      481 ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg ctagttacaa
      541 gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac tcagctatat
      601 ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag aagctgcgaa
      661 acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat ttcctgcgat
      721 ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc aattgggcat
      781 gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc accacttttc
      841 ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac ttaacgaatc
      901 aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc atcggtttgg
      961 ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat ttcctggttt
     1021 gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag gaacaaaccg
     1081 cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc gaattttatg
     1141 cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta gtaaaattta
     1201 caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt gaaataacta
     1261 gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta gcaaaaagtg
     1321 tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg gtacatggaa
     1381 catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc tacaaatcat
     1441 tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt gcaatctctg
     1501 caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag cgtttttgcg
     1561 gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg gctttttgag
     1621 ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt tgtgcaatat
     1681 aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca tttccagtat
     1741 taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt ttcataaaag
     1801 caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat gtaacgatga
     1861 cgcaatcacc ttcacggata taatttagta atttttgaaa ctcaggacgc tcagtatctt
     1921 taccactggc tttttcttgg aataatttat caatgttaag tccttcggtt ctgttgcgaa
     1981 gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta
     2041 ataccttgat ttcagtagac accgaaaagc cgaagagcgt tccatttctt cggttctttt
     2101 tatatattcc tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt
     2161 gataaaattt att
//
LOCUS       DAFELO010000145         2139 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFELO010000145
VERSION     DAFELO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.152"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(51..1598)
                     /locus_tag="LP452_002835"
     CDS             complement(51..1598)
                     /locus_tag="LP452_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL2015492.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="LP452_002836"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="LP452_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL2015493.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          607 a          444 c          379 g          709 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttg
//
LOCUS       DAFELO010000146         2104 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFELO010000146
VERSION     DAFELO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.153"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            169..1539
                     /locus_tag="LP452_002837"
     CDS             169..1539
                     /locus_tag="LP452_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBL2015494.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="LP452_002838"
     CDS             1560..1844
                     /locus_tag="LP452_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015495.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAFELO010000147         1916 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFELO010000147
VERSION     DAFELO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.154"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..1031)
                     /locus_tag="LP452_002839"
     CDS             complement(<1..1031)
                     /locus_tag="LP452_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2015496.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="LP452_002840"
     CDS             complement(1113..1802)
                     /locus_tag="LP452_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2015497.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
BASE COUNT          635 a          427 c          336 g          518 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tggctctata atttatggga ctgatgaatc agaattgatt
     1861 cttcagcccc attttcattt taggtattaa aaaacatatc gttctctagt atgatt
//
LOCUS       DAFELO010000148         1891 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFELO010000148
VERSION     DAFELO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.155"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            333..743
                     /gene="spx"
                     /locus_tag="LP452_002841"
     CDS             333..743
                     /gene="spx"
                     /locus_tag="LP452_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL2015498.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            757..1113
                     /locus_tag="LP452_002842"
     CDS             757..1113
                     /locus_tag="LP452_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL2015499.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            1787..>1891
                     /locus_tag="LP452_002843"
     rRNA            1787..>1891
                     /locus_tag="LP452_002843"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          683 a          281 c          352 g          575 t
ORIGIN      
        1 gtgacagaag tggtcagttt caagaaagaa gaaggaatgt acgaaaattg cttctcaaat
       61 ttttgtgaaa tttcagctta ttttcgaaga agctatttct ggaacacagt ttattcggaa
      121 aagggaggtg tgacaagaat tttgtcacac ctcccttatt tattttgaaa atgagaatac
      181 tattatcaaa atagagaata tatagaaaaa ttctcattat tcaaacgtaa ataatcagtt
      241 attttgattt tttttatatt ttattagcta ataattggta ttttaaaaga aattactgta
      301 gaattggatt ataattaaaa aggagtgaga aaatgataaa agtatatact tcgcctagct
      361 gtacttcctg tagaaaagca agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga
      421 agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa aaagatcttg gcagcgactg
      481 aaggaggagt tcaagatatt atttccactc ggtcgaaaat ttacgaaaag ctgggcatag
      541 attttaacga attaacttta aagcaaatga tcgtcttatt caaagaatat ccttcattat
      601 tgagacgacc tattttgatt gatgaaaaaa gaatactaat cggatttaat gaagatgaga
      661 tccggtcatt tgtccctcgt gaaattcgat gtgttgccag aaatctgatc tatttacaaa
      721 acaataacat cttaaattca tagggaggga gccttcgtgg aagtgaaagt ctatggatca
      781 gcgcactgtg taacaagtct gaaggcgaaa caatggttag aacaccatca attgccttat
      841 caatttgttg acttagaaga acgcgatcta agtacagaag aagcagaaga attatgcagt
      901 tttgaagaag tgcaagcaga acagctattt gcgacttggt ctgaggcttt tcacaacatt
      961 gcgattgacc tttcctgtgt aggaaaaaaa caactgattt ttttatgcac acaacatacc
     1021 aatctcctca gacgtccttt gattattatc gatgaagttc tatttattgg atacaatgag
     1081 cagttgatgg aacagcttat cttaaataaa tagactttaa ctgttttaat caagtcactg
     1141 agcatttcat acagttgtaa cagtcgaaca cttagcatca agcgtgttat gtgttctttt
     1201 tttgacttta tttttctact atagagacat gctcaattta tgtggtcaaa attcaaatct
     1261 aggtggggat atagagttaa aaatatattg acatgtgtaa atttacatga tatattcatt
     1321 tctgttgttt taagtagcaa agcaataaaa tagtatttta aaaaacatat aaaatagttg
     1381 ttgacatgtt agaaatgaca tgataagata actgagttgt taaaaaagaa ccacttgtga
     1441 agcttgattc tatcgaaaaa aaacttcaaa aaagtccttg acaaataaca aatgataagt
     1501 tatgataaat gagttgctgt aagatttcga aaaacgaaat caaaaaaact tttaaaaaag
     1561 agttgacaat tacttgtctc tctgatatga tataaaagtt gctacggcag ccaaaaacga
     1621 atcggggaac actcctgatg aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa
     1681 atgtgtaggg cgtcttgatt caattcaaga caacaaacat tttttaacaa gcaagcaata
     1741 tgctagcaaa caaattgagc ttaacaatcg caagattgtt cgaacttttt atgagagttt
     1801 gatcctggct caggacgaac gctggcggcg tgcctaatac atgcaagtcg aacgcttctt
     1861 tttccaccgg agcttgctcc accggaaaaa g
//
LOCUS       DAFELO010000149         1870 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFELO010000149
VERSION     DAFELO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.156"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            24..1868
                     /locus_tag="LP452_002844"
     CDS             24..1868
                     /locus_tag="LP452_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015500.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          613 a          342 c          311 g          604 t
ORIGIN      
        1 aagttttaga aaggaatacc tttatgtctt tttttaataa catatataat aaaaaagaat
       61 ctttttattt acgtataaag tggagcataa tcattatatt atcattgtta tttttaacag
      121 gaattgctta tttcttttat tttatttttt ctcctgcgga cagcaatcaa atgaaagaaa
      181 taaaagattc gtgggttttc tatacaaaag atgatcctga ttttaagttt gacagcaagt
      241 acactcgtac gattcctacc gtggataaag acgaaacatt cattatggaa acgacacttg
      301 aaaaaccgtt ggacgaagca aatttactaa tcaaaggaaa ccatcaatgg attactgtat
      361 atctaggcga aaagatttta taccaacgga aagattatca ggaagaaagc aatcccggac
      421 tctcattggc tgtgatagat ttaccagcaa attatatcgg aaaaaaattg acccttacag
      481 tctcatctcc ttatcagaac tatgctggac tgccaccgaa agtttatcta ggaacaactg
      541 gaccattgat tagttttatt tattcacaat cagtgccgca agtattgacg atgataatcg
      601 ctgtttttat cgctataggg acctttttct atacgatcta cttgatgtat aaacaaaaac
      661 gtcttgaata ctcacttatt ctcttaagtt gctttgcttt ggcattagga tttgaagctg
      721 tatcagaaga tattttaagt ggtattcttt ttgaaccatt cgttcattct tttttatctc
      781 atttatttat tatcttaacg tcagctttca tcatctgcta ctattggagc cgaatgtttt
      841 actataaaaa atggtacgga attttttgta cgttccaact tttgattttt atcggcgttc
      901 tgctttatgc aacttttact cctgcagaac ttcctgaatt aatgccgatt gctaatattg
      961 ctagtgttat aagcacttta gtcacttcac ttgcatgcat tggcgaagca tataagaata
     1021 accgttttta tgtagcttgt acaccttgga tcgtcttggt agccattatc cattgcttga
     1081 tttacatcca aacggcatta ggtatctatc aaacaacgat caattggtct acgattttat
     1141 atattatcat tctaatcgtt attatcagct acaatctaat cgatcacatc ttaaataccg
     1201 acaaagatcg gagacaagta gatttcttaa aaatcaaaaa tgatttgttg gagcaacatt
     1261 atgagcagct tcgacagcat atccaagaag taaatacact tcgcctggaa tttgtccaag
     1321 agatggagaa tctgcaagat ttaatgcata cggaccaaac aaaagctaaa aactatgtgg
     1381 aaaagatatt agaagaagca aaaaatttcg aaatgctgtg ttctttttgc aaccaccagg
     1441 tgacaaatct tatcttagcg cgttaccaga agttagctgc caaacggaat attcagataa
     1501 ccttccaagc agatcttccc gaagaacttc ctatcaacga tgacgatctg gctcaacttt
     1561 tgatccacgc tttagagcat tcctttagag aaactcatgc catcgaaaat ccactgtatc
     1621 gaaaaattta tttgtcgata cacgaacaag aggaacattt cgtcatttgt tgcgaacata
     1681 gtgcacatta cgatacaaac ctatttagtc gaggcatcac agaagaatta gatgatcagg
     1741 aacgttttga cttaatgatg atgaaagacg tagcggatcg ttacacagga acactaacgc
     1801 aggaaaaaga tactttcatt gatcggatca ctgttcaact ctctcgaaat tacccgaatc
     1861 ctgtataaaa
//
LOCUS       DAFELO010000150         1863 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFELO010000150
VERSION     DAFELO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.157"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..830)
                     /locus_tag="LP452_002845"
     CDS             complement(<1..830)
                     /locus_tag="LP452_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2015501.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     T"
     gene            complement(841..>1863)
                     /locus_tag="LP452_002846"
     CDS             complement(841..>1863)
                     /locus_tag="LP452_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL2015502.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT          459 a          402 c          327 g          675 t
ORIGIN      
        1 gtttcgactt gaaggatcgt acctggctgt tttcctaatt gaacaccttt ttcttttaaa
       61 taggcttcaa atgcttcttg tgtcgcttgg gtttcatttg gatcaagctt cgcatcaaat
      121 gcgccatttt gagcgattac ttgggctttt ccttcaaaat atggttcttt ttggaagttt
      181 ctaatctttt cctgcaattg ataaatcata tagagtgttg gggtttggac tctacctaaa
      241 gaaaaacttc ctggtatgcc tttttgctgc aagagtaaac tatacagtgg gctaccgttc
      301 atcccaatta accaatcggc gatctgacgt gcttgtactt cttcaaattt tggataggtc
      361 tcttctgcgt ctcgtaactg ttggaatccc tgataaatgg cttctttttc taaggagttg
      421 atccaaagtc ttttaaaggt ttttccttga ttggcgcctg cttgttgaat gattgaccag
      481 gcaatcagct ccccttcacg atcgctatca gtcgcgatca caatcgtatc agctgactgc
      541 agctgtcgtt tgacgacact aaactgcttt ttcttatctt taggaacatg atagtggtat
      601 tgattgggga aaataggcaa atgttctaat gcccattgtt tccatttttc atcgtacata
      661 tcgggcgaat ctaactcgac taaatgacca aaaccatacg tgatcacagt ttctcctgta
      721 aacaatcgat cttggatctc aaaataccca tcttttcggg tagctttaga aaagctatcg
      781 gcataagcct tcgcttgtga cggtttttct gctaaaatga cggttttcat agtgacgact
      841 tcactccctt tcttgttgtc tgccttgttg aaacaacatc gcacgttttt gctttctgat
      901 ttcttgttgc tggatctcat gcttcaattg atcattccaa tcctttttca cttcaggtac
      961 aaattcatgt tcatgctttt tatagccttt gtagtattgt tctgctttat aggaaagaag
     1021 gattcctgca ttaacgtctt ttatgttggc ttttctacta taaaaacgat caaagttgta
     1081 tgttccatct gcgcgttcac atactttcag ttccttcgcg accgtggtac atgcttcttt
     1141 caaatcaatt gtttctactg gttcgccctt cgccaacatc tttccaaacg tagattgaac
     1201 atcatgagca gacaccaatt gatttgtcag ctgtaaatta gtttcagttt tgtgtatggc
     1261 cataattgct tcaggttcaa cactcatttc ttttgcgact gcttcataag tagctttgta
     1321 ttctttaggt acttgccaat catttggaat tcctcgctca caacggattt tcttattggt
     1381 aaaaggatca acgattccct tcatctgttc tttttcataa aaaatatgtc ctgcacgatc
     1441 attgtccaca catatttcaa tcgattgagg aaatgtttgt tttcgtctta attcagaaat
     1501 cgactcttca acgtaatgac tgattacatg atgttttaac ccgtccatcg aaactaacca
     1561 ggcatcttgt agtttctccc gattcaatgc agcgtaactt aacagatcaa tactactttc
     1621 aaagaatttt aaatgcttcg gatcgcctat ttttaaatta aatccgtgat ttggcgtagg
     1681 attcttgtca atgtgtttat acgttccccg tttaccgaaa cgattaaaat cgacgatcgt
     1741 tccttgataa cttgccccta ctgtctctcc tgtctgtgga tctttccata aaaaagcaac
     1801 cgcatgatct aaacggtcgt ctccaactaa aaaattttgc cgttcatgtt tcggctttag
     1861 ttc
//
LOCUS       DAFELO010000151         1854 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFELO010000151
VERSION     DAFELO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.158"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..830)
                     /locus_tag="LP452_002847"
     CDS             complement(<1..830)
                     /locus_tag="LP452_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2015503.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     T"
     gene            complement(841..>1854)
                     /locus_tag="LP452_002848"
     CDS             complement(841..>1854)
                     /locus_tag="LP452_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL2015504.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT          471 a          399 c          322 g          662 t
ORIGIN      
        1 gtttcaactt ggtggatcgt acctggctgt tttcccaatt gaaccccctt ttcttttaaa
       61 taatcttcaa atgcttcttg cgtcgcttgg gtttcattcg gatcaagctt cgcatcaaat
      121 gcgccatttt gagcgattac ttgggctttt ccttcaaaat atggttcttt ttggaagttt
      181 ctaatctttt cctgtaattg ataaatcatg taaagcgttg gggtttggac tctgcctaag
      241 gaaaaacttc ctggtatgcc tttttgctgc aagagtaaac tatacagcgg gctaccgttc
      301 atgccgatca accaatcggc gatctggcgt gcttgtgctt cttcaaattt tgggtaggtc
      361 tcttctgcat ctcgtaactg ctggaatccc tgataaatgg cttctttttc taaggagttg
      421 atccaaagtc ttttaaatat ctttccatga tcggcgcctg cttgttgaat gatcgtccaa
      481 gctatcagtt ccccttcacg atcgctatct gtcgcaataa taatggtgtc ggccgattga
      541 agttgctgtt tcaccacctt gaattgcttt ttcttatctt taggaacatg atagtgataa
      601 tgtgtaggga aaataggcaa atgctctaat gaccattgtt tccaattttc atcgtacata
      661 tcgggcgaat ctaactcgac taaatgacca aaaccatacg tgatcacagt ttctcctgaa
      721 aacaatcgat cttggatctc aaaataccca tcttttcggg tagctttaga aaagctatcg
      781 gcataagcct tcgcttgtga cggtttttct gctaaaataa cggttttcat catgacgatt
      841 tcactccctt tcttgctgtc taccttgttg aaacaacatg gcacgttttt gctttctgat
      901 ctcttgttgg tagatttcat gcttcaattg atcattccaa tcctttttta cttcaggtac
      961 aaattcatgt tcatgatttt tatagccttt gtagtattgt tctgctttat aagagaaaag
     1021 aatctgagca ttaatatccc ctttttcttg ataaaaacga tcaaaatcgt acgtaccatc
     1081 cacgcgttca caagctttta attcttttgc gacttctcta caaatatctt tcaaattaat
     1141 agcttctatt gaatcgttaa cagaaagctg ctgaccaaaa gacgcattta ctttgtgagc
     1201 ggaaaccaat tggttcgtta attgcagatt gttttctgtc ttgtgtatgg ccataatagc
     1261 ttctggtgtg atcttttctt cttttgcgac ttcttcataa atgactttgt attcttttgg
     1321 cacttgccaa tcattagcga ttcctcgttc acaacgtact ttctgattcg taaatggatc
     1381 aactgcgccc attaattgtt ctttttcgta gaaaatatgt cctgcacggt cattatccac
     1441 acaaatctcg atggattgag gaaaggcttg tttctttctc aattcagata ctgcctcacc
     1501 aaaataatga ctgatcacat ggtgttttaa cccttccatc gaaactaacc acgtatcgtt
     1561 taactgatca cgatttagtg cagcatagct aagtaaatca atactactct caaaaaattt
     1621 taactgctta gggtcaccga tctttaggtt aaagccatga ttcgctgtcg aatttttatc
     1681 tatatgctta tatgtgcctc tctcaccaaa acgttcataa tcaatgaacg ttccttgata
     1741 actcgcccct actgtctcat ttgtctgtgg atctttccat aaaaaggcga ccgcatgatc
     1801 taaacgatcg tctcgaacta agaaattttg ccgttcatgt tttggcttta gttc
//
LOCUS       DAFELO010000152         1810 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFELO010000152
VERSION     DAFELO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.159"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            325..1188
                     /locus_tag="LP452_002849"
     CDS             325..1188
                     /locus_tag="LP452_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2015505.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1288..1770
                     /locus_tag="LP452_002850"
     CDS             1288..1770
                     /locus_tag="LP452_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2015506.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
BASE COUNT          629 a          281 c          394 g          506 t
ORIGIN      
        1 aaagaaaaat ccaagcagcg ttctgatcag cagtttgaac aacaatttgc tttgtacagt
       61 aggtagtaag cggattaact aagggaataa aagatatttt taccgctttg tttacttaga
      121 atctgtcttc cccaaaattt atttttctaa aggtaaaaca tagataggca gataatacca
      181 attatccgtt acgaaacgct gcttgagaat tttctttaga tccatacaac attttctaca
      241 tctgactaaa ttggttcctg attcatcaag taattgactt tctttgataa tatgataaaa
      301 aggcaatcaa ggaggaagat aaatatgctt aaagatctga accaattgat cgattacatc
      361 gaagaacatt tgacagaagg actttcctat aaaataatag ccgaaaatac aggaatctct
      421 gaatatcaat taaaacgcat ctttacgttt attgcaggga tgtcgctaat ggactatatc
      481 aaaaaccgta gattggcttt ggctaatcgc gatcttctag atggatcaag agtgacagaa
      541 acggcgttta gatatggata tgaaacagtc gaaggttttt caagagcttt tcgtgattgg
      601 tcaggatatc tgccttcaga aatcagcaga tacggcatac aaaaatcttt tccacgcttg
      661 acatttttta ttgatgtcag aggaggaaaa tcgatggaat ttcgtatcga aaagaaagaa
      721 cagttttatg tggcaggtgt ggcgaaaaaa gttcctgtca aatttgaagg agtcaatcaa
      781 gaaatacaaa agttagcgca gacgatcacg gagactcaaa gggaagagat gcataagtta
      841 ggagatttat atccaaatca agtaatcaac gtttcttatg gatttgatgg agaccgcttg
      901 gaagaaaagg gcagtttgat gcacatgatt ggctttgcta cgacaaaaga aaatccgtac
      961 gctgatttag aaatgcttgc tatagaagaa cacacttggg ccatttttcc taataaaggg
     1021 ccatttcccc aaactttaca agatacttgg gggaaaatct atgcagaatg gcttccttct
     1081 tcagattatg ggttagtcga agctcccgag atctctttta caaaatgggg agaggatttc
     1141 agtaatgttt acagtgagat ttggattgca gtgaaagcaa aaaaatagtt aaaaaataaa
     1201 aatatcccta gaaaaaagag cgtaatcgtg tcatttccct ttaattttat gtaagatgaa
     1261 cttaatataa tagaaagggt gggagaaatg gcaaagtata ggatcgaaga aaaaccagat
     1321 tttacggtga cgggtattgg tgtggaattg aagagtagca atacggattt tgtcggtttg
     1381 gctgcagaga aacaagcatt tgagcaaaaa gttatcggta atggaacaat agatgcattg
     1441 aaacaagctg ctgaaaatca ttatttcttt acagtcaacg aaccctataa cgggaaaatg
     1501 atgtactatg tcggtgttca aacgagtcag gatgttccaa acactacgcg aatcatccca
     1561 ttcccaaaag gaacgtatct tgttgtaagc gctttagcag aatcgcctga agaattaaga
     1621 aatgatttga cgaatacggc ctttgggcat gttttaagag aagtagaaga ttacgcctat
     1681 gttgggggtc caaatgctgt agtcgaaatg ggagttacca acgggaaata tatgggagaa
     1741 atctggatac cggtagtcaa taaaccatga taatatagag gtcgtgaaag tagcgctttg
     1801 acctgagcat
//
LOCUS       DAFELO010000153         1802 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFELO010000153
VERSION     DAFELO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.160"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            160..561
                     /gene="tnpA"
                     /locus_tag="LP452_002851"
     CDS             160..561
                     /gene="tnpA"
                     /locus_tag="LP452_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL2015507.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            573..1718
                     /locus_tag="LP452_002852"
     CDS             573..1718
                     /locus_tag="LP452_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2015508.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
BASE COUNT          681 a          274 c          310 g          537 t
ORIGIN      
        1 cacccgtgat tttaatcatg ggatgaatcg gattcagttg tgacctagct ttagctaggt
       61 taaacaactg acaaatataa actaaataat agtgaaatgt cttgcaataa ttgattttgt
      121 agaaatacat atattaaaat aattgcgagg tgtttttgta tgtcagaaat tcattatgga
      181 cggggctatg tctattctat tcaatatcat cttgtttggt gtgtaaaata tcgacatgat
      241 gttttgtatg gtcaggtaga tattgatgtc aaacagtttt tgaaacaaat tgctgatgat
      301 aataatataa aaattataga aatagaatca gataaagacc atattcattt actcattgaa
      361 tgcaccccac aacactatat tcctagtatt gttaaagtat ttaaaggcgc atccgctaga
      421 ctgctgttta aaaaacatcc tgaacttaaa caactccttt ggggtggaca tctttggaat
      481 ccaagctact ttgtagctac tgttagcgaa aatacagaag aacaaattag aaaatatata
      541 caaaatcaga aaaagaaatg aggtgaatcc gtatgctaca acatagggct tataaattta
      601 gaatctatcc taatcaagaa caagaaatat taatgactaa aactattggc tgttcaagat
      661 ttgtctataa ttattttttg aatttgtgga atcatgaata tacgactact ggaaaaggat
      721 tatcttataa ttcttgttct gccatgcttc cacaaatgaa aagaaatgca gagacaattt
      781 ggttaaaaga agttgacagt attgcgattc aatcctcatt gaaaaatctt tctgatgctt
      841 tttctcgctt ctttaaaaag caaaataaac gaccacaatt taaaagtaaa aaacatccta
      901 tacaaagtta tacaactaaa aatgtgaata atagtatcga aattagaaac aatttcgtaa
      961 aactacctaa gttaggattt gttaaatttg cgaaaagtca agagcccaaa gggcgaatct
     1021 taaatgctac gattcgaaaa aattcgagtg gtaaattttt tgtttccatc ctgtgtgaag
     1081 aagaaatagt tgagtttcct aaatctaatt ccgctattgg aatcgacctt ggtataactg
     1141 attttgcaat cctttctgat ggtcaaaaga ttgataacaa taaattcacg tcaaaaatgg
     1201 caaaaaaact caaacgtgaa caacgtaagt tgtcaaaacg tgcattacta gctaaaaaga
     1261 aaggcgtcaa tctctttgaa gctaaaaatt atcaaaaaca aaaacgaaaa gtagcacgac
     1321 tacatgaaaa agtaatgaat caacgcaatg actttttaaa caagttgagc acagagatta
     1381 tcaaaaacca cgatattatc tgtatagaag acttaaatac aaaaggtatg ttgcgtaatc
     1441 ataaactagc aaaaagcatt tctgatgtgt cttggtctag ttttgtgact aagttacaat
     1501 acaaagctaa ctggtacggt cggaaaatca tcaaagtaga taaatggttt ccatctagtc
     1561 aaatctgttc aaattgtgga cacaaagacg gcaagaaatc tctagaaatt cgagaatgga
     1621 cttgtcctgt ttgtcatgct catcatgacc gagatatcaa tgctagtatc aatattttga
     1681 ccgaaggtct aagattacat gtcatgggat tagcttagat ccaatcatca aaaaccgtag
     1741 gaactacggg gatagcttgg taaataagag aaaccgctgt gagtaaagaa atacgcttgc
     1801 aa
//
LOCUS       DAFELO010000154         1718 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFELO010000154
VERSION     DAFELO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.161"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..1307
                     /locus_tag="LP452_002853"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="LP452_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBL2015509.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1437..>1718)
                     /locus_tag="LP452_002854"
     CDS             complement(1437..>1718)
                     /locus_tag="LP452_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2015510.1"
                     /translation="IHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIE
                     RLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          686 a          159 c          297 g          576 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagtgagtc aagtccagac tcctgtgtaa aatgctatac aatgttttta ccatttctac
     1441 ttatcaaaat tgatgtattt tcttgaagaa taaatccatt catcatgtag gtccataaga
     1501 acggctccaa ttaagcgatt ggctgatgtt tgattgggga agatgcgaat aatcttttct
     1561 cttctgcgta cttcttgatt cagtcgttca attagattgg tactctttag tcgattgtgg
     1621 gaatttcctt gtacggtata ttgaaaggcg tcttcgaatc catcatccaa tgatgcgcaa
     1681 gcttttgaat attttggttg atcgatataa tcatgaat
//
LOCUS       DAFELO010000155         1685 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFELO010000155
VERSION     DAFELO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.162"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(45..779)
                     /locus_tag="LP452_002855"
     CDS             complement(45..779)
                     /locus_tag="LP452_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL2015511.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="LP452_002856"
     CDS             1106..1516
                     /locus_tag="LP452_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL2015512.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..>1685
                     /locus_tag="LP452_002857"
     CDS             1509..>1685
                     /locus_tag="LP452_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL2015513.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYN"
BASE COUNT          536 a          294 c          325 g          530 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataat
//
LOCUS       DAFELO010000156         1623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFELO010000156
VERSION     DAFELO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.164"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            43..675
                     /locus_tag="LP452_002858"
     CDS             43..675
                     /locus_tag="LP452_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL2015514.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(677..1582)
                     /locus_tag="LP452_002859"
     CDS             complement(677..1582)
                     /locus_tag="LP452_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2015515.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
BASE COUNT          492 a          334 c          295 g          502 t
ORIGIN      
        1 cccgcttctt tgctatacta aagaaaaacg aggtgaaccc acatgaaaaa aatcgaacct
       61 attccacctt ctcttcctct tcttacaaag ggagagtttt atttagaaga tgaggagatc
      121 ttctctggtc ttctgatcaa cgggggtgac caaagctatc tcgatgccaa aaatatggta
      181 ttaagagaga gtcatatcca aaaacttacg atgcaaaaaa cagttttacg tcattttgaa
      241 tgcagtaatg tcatttttga aaaatgtgat ttttccaatc tggagtggat tggtggcagt
      301 ttccaccaag tcgtgtttca tcaatgtaaa ttaacaggta cgaattttgc cgaaagttat
      361 cttcgagact gtactttcac tgattgtatt gcttcgatgg cttcctttag tagtacaaac
      421 ttgaaagctg tctccttcga tcattgtcaa ttagaagaca gcgaatttta tgaggtcact
      481 tggaaaaacc tttttttgtc tgacaatcag ttgaccggtt cgaactggtt tcgaacttct
      541 ctgaagggat tagactttac gactaatttc ttcacagcaa tcgctttatc tcaagattct
      601 ttggcgggac taatcgtcaa ccaagagcaa gcacttgtga tcgcccaagc ccttggtttg
      661 cagatcaaag actagatcaa cgaatccgcc gccacagtgc aataagcatc caaatagata
      721 agaccaaact gatgatcaag gcgattcccc atccgaaaac ggaatgggta aagggtaact
      781 ggagattcat tccgaagaat cctgtcacaa tagttggaac ggtcaagatc agcgaccaga
      841 ccgtcaaaaa tttcatcgtg tcattcaagt tgttattcag caaattatta taagtgccag
      901 agagctggtc taaaatctgc gaagctaagt tcgtcatttc aactgcttgt cgtgcttcga
      961 tcagcgcatc gtccaactct tctttttcct tttctgacaa cttgcgataa atagccaatg
     1021 ccttgatttg ttctaaaagt actacatttt gttttgttcc tgtaactaag taaaccaaac
     1081 cgatctctaa atctgaaagt tgcagcagat tcttgttcgt tgttttttct cttaatttca
     1141 gattaagccg ctgacgttca ctattcactt cctcgattaa aggaaaaaag gaatcagaaa
     1201 tcaaaaacaa tgtacgaaac aataaactgt atacactcat gtctggcatt cgttccaaaa
     1261 gcgatcgaat tagtcgaatc acgtactcgg tattcggcgt ggtaaatgta aacaaaccgt
     1321 cttcttttaa aataaaagcg ataggactag tttcataatg attatcgact ttttgctggt
     1381 gcggaacatt tgatacaagc agtaacgctt catcaaaaga atcgtactct acgcgcgccc
     1441 gttcattttt atctagagaa taagtcagca tctcgtcact aatgccatat tcttctttta
     1501 agtttttaac agctagcaca tcgttttgct gtacattgat ccaaaaatta cggtcttgat
     1561 taaaatagtg tttttcccgc atactctatc ctcctgacat ttgtttccag tataaagaaa
     1621 aaa
//
LOCUS       DAFELO010000157         1531 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFELO010000157
VERSION     DAFELO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.165"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            108..971
                     /locus_tag="LP452_002860"
     CDS             108..971
                     /locus_tag="LP452_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL2015516.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            1076..>1531
                     /locus_tag="LP452_002861"
     CDS             1076..>1531
                     /locus_tag="LP452_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2015517.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT          549 a          238 c          307 g          437 t
ORIGIN      
        1 ttttttcacc tttccaaacc aaggctcagc tattcaagta gataacatta tgttatgata
       61 gcaaagaaat gtattaagaa aacctttcac tatagaggga gaaaaaaatg aacgatttat
      121 tagcaactat atttacaggt attattattg atgaaaatga aaaaaactac tttgtccaaa
      181 aaaacggaca aacctttggc ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag
      241 ttgaaggatt cggttaccaa aatcaaaaac atgaaaatcg gatcactaca caaattccta
      301 aaagccgtat cggtcattac gcttttggaa tcgttactgc tacacgaaaa gatttaggtg
      361 cgtttgtcga tatcggtctt ccagataaag actttgtcgt atctcttgat gaattgccga
      421 caatgacaga gctatggcct aaaaaagggg atcgcttgat gatttccctt agagttgatg
      481 caaaagaccg tatttggggc agtttagcag atgaaaaaat attcaaatct ttaagcaaat
      541 atggaacaga agaaatgaaa aacaaagatg tgataggaac agcataccgc ttgaaattaa
      601 caggtacgta cgttttaacg gaagatttct atcttgggtt tatccatcct tctgaacgat
      661 ataatgaacc tcgtctaggc gagcagataa aaggtcgagt gattggtgtc cgaccagacg
      721 gtgtactgaa tctttcattg aagccaagag catatgaagc tatatcagat gatgcacaaa
      781 tgattttaac cattttagaa cgttctgcag atcagcaaat caactttaca gataaatcgg
      841 atccagaaga aatcatgaaa gcttttggta tcagcaaagg agcatttaag cgcgcattag
      901 gtaatctttt gaaacaagga ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa
      961 aatctaattg agaatccctt gcatgttttt cttaactagc ttataataat tttaattaac
     1021 atagtgttat atcttttttt atttgtaatt attataaatt gttggggttg agtaaatggt
     1081 catgaatgct atttcggcga tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa
     1141 gctgacaccg caaagagaag ctactctgct tgtgcttttg gaaaatgaga aagagcattt
     1201 atctgcagaa gaaatcttct ttttagtgaa aaaaaagagt ccggaaattg gacttgctac
     1261 tgtttatcga acccttgaaa tcttgacaga attgaaaatt gtcgataaag taagttttaa
     1321 tgatggatta gcccgttatg atttgagaaa agaaggcgcc aaacatttcc atcatcattt
     1381 actatgcttg aactgcggca cgatcgagga agtagaagag gatcttttac ttgaagtgga
     1441 gcaagtagta gaagaacgtt atcatttttt agtaaaagat catcggctaa cgttccatgg
     1501 aatctgtcag gaatgctatg aaaaagaaaa a
//
LOCUS       DAFELO010000158         1529 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFELO010000158
VERSION     DAFELO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.166"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..903
                     /locus_tag="LP452_002862"
     CDS             <1..903
                     /locus_tag="LP452_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBL2015518.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1529
                     /locus_tag="LP452_002863"
     CDS             916..>1529
                     /locus_tag="LP452_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL2015519.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLA"
BASE COUNT          511 a          218 c          358 g          442 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagc
//
LOCUS       DAFELO010000159         1502 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFELO010000159
VERSION     DAFELO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.167"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(312..611)
                     /locus_tag="LP452_002864"
     CDS             complement(312..611)
                     /locus_tag="LP452_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015520.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="LP452_002865"
     CDS             complement(617..847)
                     /locus_tag="LP452_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015521.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="LP452_002866"
     CDS             complement(1097..1297)
                     /locus_tag="LP452_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2015522.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          533 a          243 c          219 g          507 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aa
//
LOCUS       DAFELO010000160         1493 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFELO010000160
VERSION     DAFELO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.168"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(136..1155)
                     /locus_tag="LP452_002867"
     CDS             complement(136..1155)
                     /locus_tag="LP452_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL2015523.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1166..1372)
                     /locus_tag="LP452_002868"
     CDS             complement(1166..1372)
                     /locus_tag="LP452_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL2015524.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          413 a          310 c          254 g          516 t
ORIGIN      
        1 aaacctgtta tcgcatagct tggagtgtga ctattatggg tgatagtgaa gatatgcgat
       61 aacatcacta ttttatcgaa tgatttttat agttgtcaat atagttatgt aatgctcctc
      121 aacgaggagc tatttttatc gtttaggaat atttaaatac caacgtttgt catggaaatc
      181 ttgcgcaccg cctttagtgt ttccctctgg atcattcgtt gcccgcatca tgacatagac
      241 tttcttatta gggaagttac gcatgttaaa agatacatga tagccaacat ttccagaagt
      301 attataagct tgatttacat ctgatctata aattccatca gctcttactc gagctaattc
      361 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag caatgtgcca
      421 tccagctaca tgcaaatttg cattttcgat ttctccaaac tgatcaatgt gggcgtaatt
      481 cgttccatct gtcagtgtag gattagctgc accagctcgt gttggatcaa tgacaggctt
      541 gttttcagaa gttgttggat tttcatcggt aaatccatga gctaaatcat atgctaattt
      601 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccagat
      661 gttttgtgtt acccataaat gcgatttgat tcccggttgg ttataaggag tgtccaacgt
      721 taatggaata ccatatttca ttgctgaatc tctagccaat tcaacgtatg ccttgtagtt
      781 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactgttggc
      841 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      901 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      961 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tatactgatt
     1021 atttgctact tgtgagctac cttcatttgc gcccaaatta aattcattgt tgatagtata
     1081 ggcaaatccg ttaattggca ataaaaaaag agccgttaac aggctcatcg cagtaatagt
     1141 aattttcttt ttcatatcta tcctcctatt tatctgatga aattttctta tacgttcgat
     1201 ttgatacacc taaaatactc cctagaaatg ctccaaaacc tgtaataatc atcaccgtta
     1261 tatctgtgta ccgccagtta acaccttttc cgacaagacc gacaaaagtc gctaaagcgg
     1321 ggataactac tatagcgatc catttcaaaa tatcaaaggt tctattgttc atactatcca
     1381 cctcccttca ttggttgggc tactttcttt ttcaatcgct tgttttcttc tatcaatatt
     1441 tctctttctg aaagcaaggc atcaatttgc ccgtccagtt gttcaatttt atc
//
LOCUS       DAFELO010000161         1456 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFELO010000161
VERSION     DAFELO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.169"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            108..1040
                     /locus_tag="LP452_002869"
     CDS             108..1040
                     /locus_tag="LP452_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL2015525.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LP452_002870"
     CDS             1112..>1456
                     /locus_tag="LP452_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015526.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFELO010000162         1451 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFELO010000162
VERSION     DAFELO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.170"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            105..1406
                     /locus_tag="LP452_002871"
     CDS             105..1406
                     /locus_tag="LP452_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2015527.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DAFELO010000163         1410 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFELO010000163
VERSION     DAFELO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.171"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            96..1388
                     /locus_tag="LP452_002872"
     CDS             96..1388
                     /locus_tag="LP452_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2015528.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          460 a          287 c          256 g          407 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     1321 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     1381 tcaactaaaa aagatcaaga ggaactgatc
//
LOCUS       DAFELO010000164         1385 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFELO010000164
VERSION     DAFELO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.172"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..249
                     /locus_tag="LP452_002873"
     CDS             <1..249
                     /locus_tag="LP452_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015529.1"
                     /translation="NFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAG
                     AQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            249..743
                     /locus_tag="LP452_002874"
     CDS             249..743
                     /locus_tag="LP452_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015530.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            757..1188
                     /locus_tag="LP452_002875"
     CDS             757..1188
                     /locus_tag="LP452_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015531.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1190..1327
                     /locus_tag="LP452_002876"
     CDS             1190..1327
                     /locus_tag="LP452_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2015532.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIISKEESA
                     S"
BASE COUNT          511 a          201 c          302 g          371 t
ORIGIN      
        1 aattttgttt taccaaaggg agataaaggt gatcctggaa taaatgccac aactacagct
       61 gtagctacaa ctacagcaaa tgggttgatg tcgtccacag ataaaactaa gttggatgga
      121 attgcagctg gagcacagaa aaatccaggt aatgctacaa ctacgacagc aggtttaatg
      181 tcagcaaccg ataaagtgaa acttgatgga ttagcgaata ttacatttga gaaagtgggg
      241 acggtttaat ggctgatatt gttcaattaa aagaagacgg agttgctaaa tacttaaaaa
      301 cgcatgtaga agctattgat ggtaaagaag cattggtgca gacagatgga gaccaagcca
      361 ttgcaggaca taagaatttt tcaggttctg taacaatcaa taataaacgt gttttgacga
      421 ctgatgataa cagatatgaa gtggtaaatc tggttgtcac taacggtaat acagggacag
      481 caaagcttta tcgtgaagga aaaacagttt ctatatattt tgtggcttta aacggaaaaa
      541 gtagtggcgg gaatgattcg gttattttaa ctgttccaga aggctatcgg acaccaatta
      601 gttttgaaca actggttggg tcaatagacc gttctacttt gaacagtgct caactatcta
      661 ttggcgcaga cggagccatt aaatggagaa gaaattcaag ttatggatca gcttattcat
      721 ttgttatcac ttattcaatt taagggagga aatctaatga aagtagttta caaatcaatc
      781 aagccttacg gattcgagca aatcattttg aacaatcaag aaaatatccc tgaaaactgt
      841 acagagatta aaccaccagt tcctaactgg agaccaagat ttgattttga taaaaaacag
      901 tgggttgagt tagctactga agaagagaaa aaagggaatg ctgttgatag cgtagatgaa
      961 ttggctaata ttaaggcatt atacgaaacg ctgaaggcag aaaatgatga attgaaacag
     1021 ctaaattcta aagcaatgct aaacaatgta gcaatcaagc aagaaaatgt cttattgaaa
     1081 gaaaagtcag aaagtttagc gcagttgaat tcaaaaacga tgcttgcttc tgtacaaaat
     1141 accaaggaaa tcgaagaaat caaaaaacaa ttacaaggtg ggaagtaaca tgtattcata
     1201 tgaagatatc aaactaatgt atgactgggg ctgtttcact aacgaacaag taatggtttt
     1261 cgttccacta tgcattacag acgaagaagc agataaaatc attagcaaag aagagagcgc
     1321 atcttaattg atgtgctttt tattttgatt caaggagttg tcacatgatt aatttagggg
     1381 aatgg
//
LOCUS       DAFELO010000165         1362 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFELO010000165
VERSION     DAFELO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.173"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            82..>1362
                     /locus_tag="LP452_002877"
     CDS             82..>1362
                     /locus_tag="LP452_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL2015533.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAFELO010000166         1352 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFELO010000166
VERSION     DAFELO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.174"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            11..1350
                     /locus_tag="LP452_002878"
     CDS             join(11..452,452..1350)
                     /locus_tag="LP452_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL2015534.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DAFELO010000167         1350 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFELO010000167
VERSION     DAFELO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.175"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            200..742
                     /locus_tag="LP452_002879"
     CDS             200..742
                     /locus_tag="LP452_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015535.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="LP452_002880"
     CDS             898..1347
                     /locus_tag="LP452_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL2015536.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
BASE COUNT          540 a          159 c          262 g          389 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt
//
LOCUS       DAFELO010000168         1336 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFELO010000168
VERSION     DAFELO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.176"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            15..1310
                     /locus_tag="LP452_002881"
     CDS             15..1310
                     /locus_tag="LP452_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2015537.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          418 a          264 c          258 g          396 t
ORIGIN      
        1 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
       61 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      121 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      181 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      241 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
      301 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
      361 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
      421 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
      481 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
      541 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
      601 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
      661 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
      721 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
      781 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
      841 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
      901 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
      961 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1021 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1081 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     1141 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
     1201 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
     1261 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga
     1321 aagaggtttt cttctt
//
LOCUS       DAFELO010000169         1302 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFELO010000169
VERSION     DAFELO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.177"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..248
                     /locus_tag="LP452_002882"
     CDS             <1..248
                     /locus_tag="LP452_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015538.1"
                     /translation="FVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGA
                     QKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            248..772
                     /locus_tag="LP452_002883"
     CDS             248..772
                     /locus_tag="LP452_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015539.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            800..1207
                     /locus_tag="LP452_002884"
     CDS             800..1207
                     /locus_tag="LP452_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015540.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            1207..>1302
                     /locus_tag="LP452_002885"
     CDS             1207..>1302
                     /locus_tag="LP452_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2015541.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT          473 a          203 c          284 g          342 t
ORIGIN      
        1 attttgtttt accaaaggga gataaaggtg atcctggaat aaatgccaca actacagctg
       61 tagctacaac tacagcaaat gggttgatgt cgtccacaga taaaactaag ttggatggaa
      121 ttgcagctgg agcacagaaa aatccaggta atgctacaac tacgacagca ggtttaatgt
      181 cagcaaccga taaagtgaaa ctcgatggat taaccaatat tacatttgag aaagtgggga
      241 cggtttaatg gctgatattg tgcaattaaa agaagacgga gttgctaagt atttgaaaac
      301 tcatgccaaa ggaattgatg gtgtggaggg tgttttagta aaagccacag gtaatgaaac
      361 ggtcttagga acgaaaaatt ttaaagacgg attgcaattc aatggattgc ctgtgcaagc
      421 aggtatgatc gagcgagcta tcacactagc tgatcgaagt gatacaacaa acgtaactga
      481 tgtgaatgga aaaataatca gaatcggaaa catcgtattt cttactttta attttaaatg
      541 tggcacatgg cccgaaggat cagaaactcg ttggatttta aaaatcccgg acggtttcaa
      601 acgtgatcaa ggctatccag cgcaaaccgc gctttcacta gttagaaatg caagtcagcc
      661 agcggatgca cgtgcattca ttgatcaaag tagcatcata caagccaaat caggttcagg
      721 tagttcatat atttcaggta tgtggatcac tcaagatcct tggccggctt aacagattac
      781 taattggagg aagagtaaaa tgaaaacaat ttataaagta ttgtatccaa tgggatatga
      841 agaacatcaa gtagaagata actttccaac atctttacca tttgtcgaag ttccacctat
      901 tttgtttgaa aagaaggaag atgaaacgga tgaaggtttc ggaagaagac aacagtcgca
      961 attctttaac ttcactgaaa ataagtggga agaagcagtt acacaagatt attcaaaaaa
     1021 attagaacta ctagaaaatt tatctatagg tttacaggta gataatgctg cattgaaaaa
     1081 atcaaatgaa gagttaacgg aaaaagcaga ttcgatggcg cagttgaacg ccaaactgat
     1141 gcttaatgat ttaacaatta ataaaaaaat tgaagcaata gaaaaacaaa ttggaggtgc
     1201 ttcataatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg ttttactgac
     1261 gatcaagttc gacaattcgt tccactatgc attacagacg aa
//
LOCUS       DAFELO010000170         1288 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFELO010000170
VERSION     DAFELO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.178"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(14..1201)
                     /locus_tag="LP452_002886"
     CDS             complement(14..1201)
                     /locus_tag="LP452_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL2015542.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          375 a          277 c          218 g          418 t
ORIGIN      
        1 caggttcatg gtgctaaatg ttattcgaat taaattctaa atattttcgt ggagaagcca
       61 gccactcagt gtctttttcc atcaaaagtg ttccaattat ccgagtgatg gaattgatat
      121 ttggaaaaat ccgaatcact cgttctctcc ggcgaatctc ttcgttcaac cgttccaaca
      181 cattggttgt acgaatcctt cggcggatat tttcaggaaa agacaaaatt tgaatagcat
      241 cttcaaagcc gttttctaaa atgtcgcaag cagcacttaa ttttgaatca caaccatatt
      301 tttccaagaa gtgctctttt ctttcacgtg tcaattcaag ttcagaggct ttaaagatac
      361 tcttcaactc gtcttttaca tcagaagaaa ctttctttgg cagcttatca aaaatatttc
      421 tcaaaaagtg ggcttggcat ctttgccagc tcgcacctaa aaagttcttg cgaacagcct
      481 taactaaccc tttgtgtgca tcagaaatga ccagttttac accgaacaaa cctcgttgaa
      541 cgaacgattg aaaaacggta tcccaattat cttccgattc gccgtcttga acatcaaaac
      601 caagaatcgt acgtcgtcca tcagaatcaa tgccgatacc aatatataca ccttttgaga
      661 caatacgatg attttccctt actttcatat acagggcatc acacatgaaa aatggatatt
      721 ccgagccttc taggctccgg tttctccact caaaaacaaa tggatccaac tgtttcatca
      781 gatttgaaac gaatgattta gaaacagtcg caccatccgt tagcagttca actgttttag
      841 ttactttgcg agtggagaca ccagaaatga ccatttcaat catggcagcg atcaaagctt
      901 tttcgcttcg ctgatatctt tcaaagatag taggactaaa cttaccgtct ctggttcgtg
      961 gaacatgaag ttctaacgta ccaattcgag ttgtaaacga acgatcgtag taaccatttc
     1021 tttgtgattg ccgttcttct gaacgttcat aggcatttgc ctgaatatat tgatttcgtt
     1081 gttcttccat cagctgatta aacatgatag tcataagtgt ttttgcggta tcattcgcac
     1141 cgctttcgtt tataattgct tgaatttctt cgctttcaaa agtaaaatgt acttgagtca
     1201 ttgatatcag tcctattcga gtatgttttc tgcaaaaaac attgtactcc taactgagat
     1261 caatggcttt tttgatgctt cactttta
//
LOCUS       DAFELO010000171         1241 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFELO010000171
VERSION     DAFELO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.179"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            432..764
                     /locus_tag="LP452_002887"
     CDS             432..764
                     /locus_tag="LP452_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL2015543.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          414 a          187 c          272 g          368 t
ORIGIN      
        1 cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc
       61 ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg
      121 ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag
      181 tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat
      241 gcctaagcac aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa
      301 attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc
      361 aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg
      421 tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta
      481 aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg
      541 ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt
      601 acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc
      661 caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag
      721 tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata
      781 acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt
      841 agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga
      901 cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt
      961 ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt
     1021 tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct
     1081 agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac
     1141 aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga
     1201 tcaatcgagc atttaccgct gtctactagg gtttctcgtt t
//
LOCUS       DAFELO010000172         1222 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFELO010000172
VERSION     DAFELO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.180"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            23..1201
                     /locus_tag="LP452_002888"
     CDS             23..1201
                     /locus_tag="LP452_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL2015544.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          447 a          227 c          205 g          343 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa aacgtcaaac
      541 cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca ctaaagaagt
      601 actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc tgctacagga
      661 tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct taaaaggcat
      721 gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattgtt gtatccatgt
      781 atctcgtaac atcgctcata aagtacgtgt caaagaccga aaagaaatct gtgatgactt
      841 taaggctgtt tatcaagcta actcaaaaga agaagcgaat accttcttat ccggcatgat
      901 tgagaaatgg aagaaaaact atcctaaagt gacgcagtca ctcatagaaa accaagactt
      961 attaactttt tatgattttc cacctagcat tcgtagaacc atttactcaa ccaatctaat
     1021 cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt ttcaaaatga
     1081 agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa aatttctaaa
     1141 cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt ttactgagta
     1201 actaattatt ttgcaggagg ac
//
LOCUS       DAFELO010000173         1193 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFELO010000173
VERSION     DAFELO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.181"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="LP452_002889"
     CDS             <1..1076
                     /locus_tag="LP452_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2015545.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFELO010000174         1188 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFELO010000174
VERSION     DAFELO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.182"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="LP452_002890"
     CDS             141..398
                     /locus_tag="LP452_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015546.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LP452_002891"
     CDS             complement(630..1061)
                     /locus_tag="LP452_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL2015547.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DAFELO010000175         1134 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFELO010000175
VERSION     DAFELO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.183"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            55..>1134
                     /locus_tag="LP452_002892"
     CDS             join(55..331,331..>1134)
                     /locus_tag="LP452_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2015548.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DAFELO010000176         1106 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFELO010000176
VERSION     DAFELO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.184"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(57..350)
                     /locus_tag="LP452_002893"
     CDS             complement(57..350)
                     /locus_tag="LP452_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL2015549.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="LP452_002894"
     CDS             complement(378..1043)
                     /locus_tag="LP452_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL2015550.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
BASE COUNT          318 a          245 c          166 g          377 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaa
//
LOCUS       DAFELO010000177         1091 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFELO010000177
VERSION     DAFELO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.185"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LP452_002895"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LP452_002895"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL2015551.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DAFELO010000178         1061 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFELO010000178
VERSION     DAFELO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.187"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(36..992)
                     /locus_tag="LP452_002896"
     CDS             complement(36..992)
                     /locus_tag="LP452_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015552.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAFELO010000179         1020 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFELO010000179
VERSION     DAFELO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.188"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            128..>1020
                     /locus_tag="LP452_002897"
     CDS             128..>1020
                     /locus_tag="LP452_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2015553.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWK"
BASE COUNT          369 a          154 c          194 g          303 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
//
LOCUS       DAFELO010000180         1002 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFELO010000180
VERSION     DAFELO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.189"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            52..307
                     /locus_tag="LP452_002898"
                     /pseudo
     CDS             52..307
                     /locus_tag="LP452_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1002
                     /locus_tag="LP452_002899"
                     /pseudo
     CDS             371..1002
                     /locus_tag="LP452_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt ac
//
LOCUS       DAFELO010000181         1000 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFELO010000181
VERSION     DAFELO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.190"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..260
                     /locus_tag="LP452_002900"
     rRNA            <1..260
                     /locus_tag="LP452_002900"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            355..470
                     /gene="rrf"
                     /locus_tag="LP452_002901"
     rRNA            355..470
                     /gene="rrf"
                     /locus_tag="LP452_002901"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            484..556
                     /locus_tag="LP452_002902"
     tRNA            484..556
                     /locus_tag="LP452_002902"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:517..519,aa:Val,seq:tac)
     gene            560..632
                     /locus_tag="LP452_002903"
     tRNA            560..632
                     /locus_tag="LP452_002903"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:593..595,aa:Lys,seq:ttt)
     gene            640..721
                     /locus_tag="LP452_002904"
     tRNA            640..721
                     /locus_tag="LP452_002904"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:674..676,aa:Leu,seq:tag)
     gene            739..811
                     /locus_tag="LP452_002905"
     tRNA            739..811
                     /locus_tag="LP452_002905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:772..774,aa:Thr,seq:tgt)
     gene            823..894
                     /locus_tag="LP452_002906"
     tRNA            823..894
                     /locus_tag="LP452_002906"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:855..857,aa:Gly,seq:gcc)
     gene            911..996
                     /locus_tag="LP452_002907"
     tRNA            911..996
                     /locus_tag="LP452_002907"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:945..947,aa:Leu,seq:taa)
BASE COUNT          261 a          214 c          280 g          245 t
ORIGIN      
        1 aggagctgtc cttagtacga gaggaccggg atggacttac cgctggtgta ccagttgttc
       61 tgccaagggc attgctgggt agctatgtag ggaagggata aacgctgaaa gcatctaagt
      121 gtgaagccca cctcaagatg agatttccca tttctttaag aaagtaagat ccctgagaga
      181 tgatcaggta gataggtcag gagtggaagt acagtgatgt atggagcgga ctgatactaa
      241 tcgatcgagg acttaaccaa taggttaaaa gaaaagaact cggaaaaaaa cttatgtgac
      301 ttcaaatcca gttttgagtg aacaaaattt actcaataaa agataacacc acagtgtggt
      361 ggcgatagcg agaaggatac acctgtaacc atgccgaaca cagaagttaa gcttcttagc
      421 gccgattgta gtgaggggtt gccccttgtg agagtaggac gtcgccacgc ttaagttatc
      481 catggaggtt tagctcagct gggagagcat ctgccttaca agcagagggt cagcggttcg
      541 atcccgttaa cctccatatg agccgttagc tcagttggta gagcatctga cttttaatca
      601 gagggtcaca ggttcgagcc ctgtacggct catatttttg cgggtgtggc ggaattggca
      661 gacgcactag atttaggatc tagcgcctta cggcgtgggg gttcaagtcc cttcacccgc
      721 ataatttaag ttttacatgc cggcttagct cagttggtag agcatctgat ttgtaatcag
      781 agggtcgagg gttcaagtcc tttagccggc atctttaaac acgcggaaat agctcagtgg
      841 tagagcacca ccttgccaag gtgggggtcg cgggttcgaa ccccgttttc cgctttgcca
      901 aagcaatcgc gccggggtgg cggaactggc agacgcacag gacttaaaat cctgcggtga
      961 gtgatcaccg taccggttcg attccggtcc tcggcatttc
//
LOCUS       DAFELO010000182          963 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFELO010000182
VERSION     DAFELO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.191"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            50..>963
                     /locus_tag="LP452_002908"
     CDS             50..>963
                     /locus_tag="LP452_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL2015554.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLK"
BASE COUNT          395 a          118 c          161 g          289 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaa
//
LOCUS       DAFELO010000183          960 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFELO010000183
VERSION     DAFELO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.192"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..>960
                     /locus_tag="LP452_002909"
     rRNA            <1..>960
                     /locus_tag="LP452_002909"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          247 a          222 c          294 g          197 t
ORIGIN      
        1 agaatcctaa ggtgagcgag agaactctcg ttaaggaact cggcaaaatg accccgtaac
       61 ttcgggagaa ggggtgctga tcatacgatc agccgcagtg aataggccca agcgactgtt
      121 tatcaaaaac acaggtctct gcaaaatcgt aagatgaagt ataggggctg acgcctgccc
      181 ggtgctggaa ggttaagagg agtgcttagc gcaagcgaag gtacgaattg aagccccagt
      241 aaacggcggc cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc
      301 gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa cgagagactc ggtgaaattt
      361 tagtacctgt gaagatgcag gttacccgcg acaggacgga aagaccccat ggagctttac
      421 tgtagtttga tattgagtgt ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc
      481 ggaacgctag tttcgatgga ggcgctggtg ggatactacc cctgcgttat ggccactcta
      541 acccgcacca ctaatcgtgg tgggagacag tgtcagatgg gcagtttgac tggggcggtc
      601 gcctcctaaa aggtaacgga ggcgcccaaa ggttccctca gaatggttgg aaatcattcg
      661 aagagtgtaa aggcagaagg gagcttgact gcgagaccaa caagtcgagc agggacgaaa
      721 gtcgggctta gtgatccggt ggttccgcat ggaagggcca tcgctcaacg gataaaagct
      781 accctgggga taacaggctt atctccccca agagtccaca tcgacgggga ggtttggcac
      841 ctcgatgtcg gctcgtcgca tcctggggct gtagtcggtc ccaagggttg ggctgttcgc
      901 ccattaaagc ggcacgcgag ctgggttcag aacgtcgtga gacagttcgg tccctatccg
//
LOCUS       DAFELO010000184          947 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFELO010000184
VERSION     DAFELO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.193"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            40..264
                     /locus_tag="LP452_002910"
     CDS             40..264
                     /locus_tag="LP452_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL2015555.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            261..>947
                     /locus_tag="LP452_002911"
     CDS             261..>947
                     /locus_tag="LP452_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2015556.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGA
                     ANPTLTDGTNH"
BASE COUNT          319 a          159 c          200 g          269 t
ORIGIN      
        1 cacgcaacaa attaaaactt tatttaggga gagaagaaaa tgaataataa aacgttcgaa
       61 gtactaaaat ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt
      121 ggtaaagcgc tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc
      181 gcgtttttag ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa
      241 agcgaagaag gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcctgttaa
      301 cggctctttt tttattaccc attaatacgt ttgcttatac tattaatgac gagtataatt
      361 tagcgccgaa tcaaggagac tccagattag caattcctaa caaaattatt ttgcacgaaa
      421 ctggaataga tgcaccagca agaaacgtag ccgccaacat gaaaaataat tataacggaa
      481 gcaattctta tactacagat gttattggtg acggtgggat tgtttaccgt gtgggtgagc
      541 aaggatatgt ttcgtgggga gctggtaacg ctaatcctta tgcgcctgta cagattgaat
      601 tacagcgcac atatgataaa gcattgtttg aaaaaaacta tcgagcttat attgaatata
      661 caagagatag tgcaaaaaaa tatggaattc cattgactct tgatcaagga acttctttat
      721 ttacaaaagg aatcatttct catttgtggg tgacaaatta tgtttggggg aaccacacag
      781 atccatatgg ttacttatcg caaatgggag ttagcaaaga aaagcttgct tatgatttag
      841 ctcatggatt taccgatgaa aatccaacta cttcagatga taaaccagtc attgatccaa
      901 ctagagcagg tgcagcaaat cctacgctga cagatggaac aaatcac
//
LOCUS       DAFELO010000185          908 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFELO010000185
VERSION     DAFELO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.194"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..750
                     /locus_tag="LP452_002912"
     CDS             <1..750
                     /locus_tag="LP452_002912"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2015557.1"
                     /translation="SIYIASESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNE
                     VWTWDITWLKGPIKGLFYRLYLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERI
                     QGQPLVLHSDNGSPMKAATFQVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEY
                     PNNGFKTIDDARKWTKEFVDWYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDA
                     KKRHPERWSKGVRNWTPHQSVALNPLKETES"
BASE COUNT          300 a          179 c          192 g          237 t
ORIGIN      
        1 tcgatttata ttgcatcaga gtctagcttc tatcgtgtat tgcgagaaaa aaatatgcaa
       61 catcaccgtg gccgaagtca agtttcacaa aaacgcatac cgccaagtca ccttgcgacg
      121 aagccaaatg aagtttggac ttgggatatt acttggctaa aaggtccaat aaaaggactc
      181 ttttaccggt tatatctcat cattgattta tttagccgta aaatggtagg ctgggagatt
      241 tgggaatcag aagaagctcg tcatgccgaa aaacttatta aacagacggt gcttaaggaa
      301 cgtatccaag gacagccgtt ggttttacac tccgataatg gcagccctat gaaagccgcg
      361 acttttcaag tcttactcga aaaattagga attcaaagtt cttattcaag accacgagtg
      421 agtaatgaca atccttattc ggaagcggct ttccgaacgt taaaatatcg accagaatat
      481 cccaataacg gctttaaaac aatagatgat gctcgaaaat ggacgaaaga gtttgtcgat
      541 tggtataacg attctcattt gcatagtggg attcaatttg tgtctcctgc agcgtgtcat
      601 gaaggacgac atctcgctat tttagagaaa cgaaagcagg tttacgagga cgctaaaaag
      661 cgccatcctg aacgctggtc aaaaggtgtt cgaaattgga ctccacatca atcggtggcg
      721 ttgaatcctt taaaagaaac agaaagttaa caggagcata atctttcaag attatcaaat
      781 aaccctcgtc acccaatagg ggaaaaagtt attcttcccc tattggataa cgaggccaac
      841 cgaccatagg gaggttctgc tttttgatag gtggaaaata aaaatacgac aactatattg
      901 acaaacac
//
LOCUS       DAFELO010000186          891 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFELO010000186
VERSION     DAFELO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.195"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..>891
                     /locus_tag="LP452_002913"
     CDS             <1..>891
                     /locus_tag="LP452_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBL2015558.1"
                     /translation="PRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDT
                     PYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMT
                     AAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGW
                     KKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAG
                     KTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVE
                     KEPLLTSAEMTAKWESYLLKI"
BASE COUNT          335 a          169 c          195 g          192 t
ORIGIN      
        1 ccacgtcttt tttctctatc cagccttcaa tctaaaatga atcagctcat gaaagctagt
       61 gccaaagata ctttggaagc catgcaaggg ttgtatgaag ggaaatatct aagctaccca
      121 cgaacagata ccccttatat tacagaaggg gagtatgcgt atctgttgga tcacttagac
      181 gaatacaaac actttttaaa ggccgaagcc attcctactc ctattcatac accgaatagt
      241 cgatatgtga ataataagaa agtccaggaa cattacgcga tcattcctac aaagacggtc
      301 atgacggcag cggcttttga acaattaagt ccactgcagc aggctattta tgaacaagtg
      361 ttaaaaacaa cggtggccat gtttgcggaa aagtatacct atgaagaaac aacgatcttg
      421 acgcaagttc agcagcttca gctaaagaca gtcggaaaag taccagtaga tctaggttgg
      481 aaaaagctgt ttggcaaaga gagcgacaga aaagaaaagg aagaagaacc gctacttcct
      541 aaagtgacaa aaggagaagc ggtgacggta gatctacaag tccttgaaaa agaaacgaag
      601 ccaccacaac cttataccga aggtacgttg atcacagcga tgaaaacagc tggaaaaacc
      661 gtggatagcg aagaagcaca atccatattg aaagaagtag aaggaatcgg tacagaagca
      721 acacgagcaa atattattga aactttgaaa caaaaggaat atatcaaggt agaaaaaaat
      781 aagcttgtgg tgacaaataa agggatttta ctttgccaag ccgtggaaaa agagccgctg
      841 ctaacaagtg ccgaaatgac cgccaaatgg gaaagttact tattaaagat t
//
LOCUS       DAFELO010000187          865 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFELO010000187
VERSION     DAFELO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.196"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            136..822
                     /gene="gpmA"
                     /locus_tag="LP452_002914"
     CDS             136..822
                     /gene="gpmA"
                     /locus_tag="LP452_002914"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL2015559.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT          308 a          163 c          156 g          238 t
ORIGIN      
        1 ttttttatat tctctacttg ttttctcata gaatcttatc taatttttcg atggaaatct
       61 cttccaaaaa attgtacaat aaaacagaac tgaaaatttc agaaatataa acctatctta
      121 aaaggagagc taaacatgcc aaaattagtt ttttctcgtc acggtcttag tgaatggaat
      181 gcattgaacc aatttactgg ttgggctgat gtaaatctag caccagaagg aatcgaagaa
      241 gcaaaagaag gcggacgcaa aatcaaagaa gctggaatcg aatttgatgt tgcttacact
      301 tctgttttaa ctcgtgcaat caaaacatgt aacttgatct tagaatattc agatcaactt
      361 tgggtaccac aaatcaaatc atggcgctta aacgaacgtc actatggtaa attacaaggt
      421 ttgaacaaaa aagaaacagc tgaaaaatac ggcgacgaac aagtgcacat ctggcgtcgt
      481 tcttacgata ctcttcctcc attaatggaa gaaactgacg aaggttctgc agcaaatgac
      541 cgtcgttacg caatgttaga caaacgcgat attcctggtg gagaaaactt gaaagtgaca
      601 ttagaacgcg ctttgccatt ctggcaagat gaaattgctc cagcattact agacaacaaa
      661 actgttttag tagctgcaca tggtaactca ttacgcgccc tagctaaaca tatcgaaggt
      721 atctctgacg aagacatcat ggatcttgaa attccaacag gtcaaccact tgtttatgaa
      781 ttaaatgatg acttaacagt tgctaaaaaa tactacctat aaaaaacact ataaaaaagg
      841 gactgtgaca cgatttgtca cagtc
//
LOCUS       DAFELO010000188          861 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFELO010000188
VERSION     DAFELO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.197"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            554..>861
                     /locus_tag="LP452_002915"
     CDS             554..>861
                     /locus_tag="LP452_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2015560.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     L"
BASE COUNT          308 a          164 c          139 g          250 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata tagataaagt ccgtataatt gtgtaaaagt
      481 aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa aacataccca
      541 ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga ttcaaagcat
      601 tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaacaacgg tatttaatca
      661 actaatggaa aatcaacgaa cagaatatat tcaagcaaaa gaatatgaac gaacagaaaa
      721 ccgacaaagt caacgaaatg gctattatga gcgcagcttt acgacacgtg taggcacgct
      781 agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt ttgaacgtta
      841 tcaacgaaac gaaaaagccc t
//
LOCUS       DAFELO010000189          852 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFELO010000189
VERSION     DAFELO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.198"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(1..152)
                     /locus_tag="LP452_002916"
                     /pseudo
     CDS             complement(1..152)
                     /locus_tag="LP452_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(429..626)
                     /locus_tag="LP452_002917"
     CDS             complement(429..626)
                     /locus_tag="LP452_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2015561.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          284 a          159 c          116 g          293 t
ORIGIN      
        1 gtgatgacgg cacttttatg gtggatgccg acaatcgtat caccctctaa atgtccaaat
       61 tcttcttcga aaacgacata atctttttta cgctcagaaa tatttctttt gaatgcctgt
      121 tttccacgtt tttccttata accatttcat tgtgatccac ctcttttgtt tattagttta
      181 aaccatcaaa ccatttttaa taaaatatgt aaattccttt gctcaaaaaa gtagcttctt
      241 tacgattcga caatttcaaa taaagcaggt aaaaactggc tgtcagaaaa tagccaaact
      301 gaagcaaaaa ataatgttaa aattaattaa tgaacgaaaa aaatccctca cctaatatta
      361 cggtgaggaa ttttttctac cgacataaac aaaaaaacca cctcatatga ggcggttaac
      421 gttcaacatt attttacgat atttactgct tgaggaccac gttgtccttc ttcaatatca
      481 taagtaactg cttggccttc atctaaagac ttaaagcctt ctccttggat tgctgagaaa
      541 tgagcgaata cgtcatttcc atcttcacca gtgatgaaac caaaaccttt atctgagtta
      601 aaccatttta ctgtaccgtt attcatatat atttcctcct gatacgtata taatacgtgt
      661 tttattgcaa ttaatattac ttggtaaaag gagtttttat agtgtgaata tatcgctcag
      721 attacgtttc aaattagatt acttatttac tataacacgg atgtaatgct aacacaagga
      781 taatcactcg atatacggtt taaatattat tatttgtcaa attaagcgag acaaaacttt
      841 ccatttacgt aa
//
LOCUS       DAFELO010000190          791 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFELO010000190
VERSION     DAFELO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.199"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..782
                     /locus_tag="LP452_002918"
     CDS             <1..782
                     /locus_tag="LP452_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2015562.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAFELO010000191          775 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFELO010000191
VERSION     DAFELO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.200"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(249..728)
                     /locus_tag="LP452_002919"
     CDS             complement(249..728)
                     /locus_tag="LP452_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015563.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DAFELO010000192          756 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFELO010000192
VERSION     DAFELO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.201"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..>756
                     /locus_tag="LP452_002920"
     CDS             <1..>756
                     /locus_tag="LP452_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBL2015564.1"
                     /translation="FITFKEIMRTVSNIYHNCVPDKIKNRRNTDQLKQRDTVIIACVI
                     WGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKG
                     ELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPIT
                     YSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDK
                     SEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNS"
BASE COUNT          267 a          140 c          127 g          222 t
ORIGIN      
        1 cttttataac attcaaggaa attatgagaa ctgtttctaa catataccac aattgtgtac
       61 cagataagat taaaaaccga agaaatactg atcaactgaa gcaacgcgat acagtgatta
      121 ttgcttgtgt tatatggggc ataattaatg gctatactag ccaaagagcc acgtatagag
      181 cggtttgttc cgtcttattt cctaatggtg actttcctag taggagtcga ttcactcgct
      241 taagttcaaa cttagcttac actatcaaga ttattcgata ctttttcatc aagaagctca
      301 ccaaaggtga actagtcgga attatagata gttttcctag tccattatgt aaaccagtta
      361 gaaatagaca agcaaaacta ttgaatcaaa tagccaaagt tggctataac tcaacaaaaa
      421 aatcttattt ttatggtctt aaaattcata tgatcgttac taagactggc tttccgatta
      481 cttactcaat cacgaatcca ggtgtccatg acgtgaaagt gttagagact ttatcggaag
      541 aagcgaatct tcccaatatc ctaggggaca aaggatatat tagccataaa atccatgaaa
      601 agcttgccct taagggcatt actatatccg ttccgccacg taaaaacatg gataaatcag
      661 aaaaactaga ccacagctta cttggaaaac aaagaaaaac agttgagact gttttttctt
      721 ccttagaaaa attaggttgt caaaatttca actcac
//
LOCUS       DAFELO010000193          698 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFELO010000193
VERSION     DAFELO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.202"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
BASE COUNT          247 a          116 c           85 g          250 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta atggaatggc aacagttt
//
LOCUS       DAFELO010000194          689 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFELO010000194
VERSION     DAFELO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.203"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..>689
                     /locus_tag="LP452_002921"
     rRNA            <1..>689
                     /locus_tag="LP452_002921"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          204 a          137 c          209 g          139 t
ORIGIN      
        1 attcgattcc ctgagtagcg gcgagcgaaa cgggaaaagc ccaaaccagc aagcttgctt
       61 gttggggttg taggactcca atatggtagt tctttcagat agtcgaatga cttggaaaag
      121 tcagtcaaag agggtaaaaa ccccgtagac gaaatgtgga agacacctag gaggatcctg
      181 agtacggcgg aacacgagaa attccgtcgg aatccgggag gaccatctcc caaggctaaa
      241 tactccctag tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga
      301 aggggagtga aatagaacct gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg
      361 tgatggcgtg ccttttgtag aatgaaccgg cgagttacga ttgcatgcga ggttaagttg
      421 aagagacgga gccgcagcga aagcgagtct gaatagggcg tttgagtatg tagtcgtaga
      481 cccgaaacca tgtgatctac ccatgtccag gttgaaggtg cggtaaaacg cactggagga
      541 ccgaacccac gtacgttgaa aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa
      601 cgaacttgga gatagctggt tctctccgaa atagctttag ggctagcctc ggaattgaga
      661 atgatggagg tagagcactg tttggacta
//
LOCUS       DAFELO010000195          652 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFELO010000195
VERSION     DAFELO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.205"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(21..251)
                     /locus_tag="LP452_002922"
     CDS             complement(21..251)
                     /locus_tag="LP452_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015565.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          224 a           96 c           94 g          238 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa ac
//
LOCUS       DAFELO010000196          625 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFELO010000196
VERSION     DAFELO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.206"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..140
                     /locus_tag="LP452_002923"
     CDS             <1..140
                     /locus_tag="LP452_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015566.1"
                     /translation="IQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKER
                     V"
     gene            201..>625
                     /locus_tag="LP452_002924"
     CDS             201..>625
                     /locus_tag="LP452_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015567.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT          248 a           97 c          118 g          162 t
ORIGIN      
        1 agattcaaac ggcgttacct tcagaagaat ttgcgctagg aaatccattc cgattttcac
       61 ttgaaacagt gtcagaagtg gccaaacgaa aaaatgaaat ctattatgaa caacaattgt
      121 cacaaaaaga acgcgtataa aacaagtaaa aaagactatc tgaaaaacgg atagtctttt
      181 ttcttttggg aggtttgaaa atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac
      241 agatcgatat agtggatttt gctcgaaaca atggaatggc tgtagtcaat aaaggacgag
      301 actatcgctt agaagaccat gattcatttg tttttgatcg aagaaaacaa cgtttttatt
      361 ggaacagtca aaatattagt ggcgacatta ttgaattagc taaacttttt ttcatagaca
      421 aagagatcca agactctaag caacaattta aagcagccac agattttatt ctaaagaatg
      481 aagacaaaac agaacgagta gaaaatcttc attttgagac agaaaagtac aaagatcacc
      541 ccgttgatta tcaaccactc acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac
      601 taccagagtg gctgattgat tatgc
//
LOCUS       DAFELO010000197          621 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFELO010000197
VERSION     DAFELO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.207"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(<1..214)
                     /locus_tag="LP452_002925"
     CDS             complement(<1..214)
                     /locus_tag="LP452_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBL2015568.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGN"
     gene            complement(201..530)
                     /locus_tag="LP452_002926"
     CDS             complement(201..530)
                     /locus_tag="LP452_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015569.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          188 a          133 c           66 g          234 t
ORIGIN      
        1 cattaccttc atcatcttca ggtagcaagc cgaatccaat tggattatca gtcaatgttt
       61 gatcgtataa atcatcctcg tctaatgcta aaacatctaa tcctttacaa tttccgcctc
      121 ttctaaacgt cacaaatcct ttgtaatccc aacgttggaa agaaacctta atcgtatgtg
      181 tgcccaatcg tatattcgga tcataatttt tcatttgttg taacctcctc ataatcagtt
      241 agtactataa aggtatcttt gtttttatcc aaaaaatcta ataaatcttt tgtagaagat
      301 atctcttcat caacttcacc ataataaata ccttcgataa ttgttgctat atctgaaaaa
      361 ctaattttta ctggacattt atcgtctacc gcattcaata ccgacaaaat tgcttctttt
      421 tctgcaatct ctcgtttgaa tttctttgga gctaatcgca tgacactcaa gtcattttca
      481 agcttttcaa tccatgcagt tttactcatt ttcgcaaatt tttgaatcat tcctctacca
      541 cctttgctat ctactaaatt agttttactt cataacgaat atctttatcg tttttcgttc
      601 actcaaaaat atataaaata a
//
LOCUS       DAFELO010000198          615 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFELO010000198
VERSION     DAFELO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.208"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            75..>615
                     /locus_tag="LP452_002927"
     CDS             75..>615
                     /locus_tag="LP452_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015570.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQ"
BASE COUNT          215 a           78 c          138 g          184 t
ORIGIN      
        1 ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa
       61 gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta
      121 aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat
      181 caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag
      241 ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg
      301 gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt
      361 tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta
      421 ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa
      481 agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag
      541 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag tcatatgtag
      601 atcaaaaatt ccaag
//
LOCUS       DAFELO010000199          558 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFELO010000199
VERSION     DAFELO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.211"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            53..>558
                     /locus_tag="LP452_002928"
     CDS             53..>558
                     /locus_tag="LP452_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HBL2015571.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQ"
BASE COUNT          196 a           91 c          114 g          157 t
ORIGIN      
        1 aaaaaatcta ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca
       61 ttttaaaggc aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg
      121 ttacaatcta agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg
      181 tcatactacg atttatcgtt gggtgcaaga gtacagcaaa gtcctctatt atctttggaa
      241 gaagaaaaat agacaatcct tctattcatg gaaaatggac gaaacctata tcaaaattaa
      301 gggacgttgg cattatcttt atcgtgcaat tgatgcggac ggcttaacct tagatatctg
      361 gttacgaaag aaacgggata cgcaagcagc ctatgctttc ttaaaacgac tccataaaca
      421 gtttggtgag ccgaaagcaa ttgtgaccga taaagcacct tctcttggct ccgcctttag
      481 aaagttacag agtgtgggtt tatatactaa gacagagcac cgaactgtga agtatcttaa
      541 caatttaata gaacaaga
//
LOCUS       DAFELO010000200          519 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFELO010000200
VERSION     DAFELO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.denovo.213"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..>519
                     /locus_tag="LP452_002929"
     CDS             <1..>519
                     /locus_tag="LP452_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2015572.1"
                     /translation="LFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNE
                     FGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLF
                     KSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFN
                     SVDEFFIQSVAS"
BASE COUNT          177 a           87 c          100 g          155 t
ORIGIN      
        1 ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag
       61 aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc
      121 aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa
      181 gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg
      241 gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa
      301 acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg aaacagatca
      361 gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct caaagaggcc
      421 taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct atggatttca
      481 attcagtaga tgaatttttt attcaatctg tcgcatcta
//
LOCUS       DAFELO010000201        21454 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.047, whole genome shotgun sequence.
ACCESSION   DAFELO010000201
VERSION     DAFELO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.047"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            288..404
                     /locus_tag="LP452_002930"
     CDS             288..404
                     /locus_tag="LP452_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL2015573.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            420..2339
                     /gene="tet(M)"
                     /locus_tag="LP452_002931"
     CDS             420..2339
                     /gene="tet(M)"
                     /locus_tag="LP452_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL2015574.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2437..2625
                     /locus_tag="LP452_002932"
     CDS             2437..2625
                     /locus_tag="LP452_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL2015575.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2683..3036)
                     /locus_tag="LP452_002933"
     CDS             complement(2683..3036)
                     /locus_tag="LP452_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2015576.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3567..3989
                     /locus_tag="LP452_002934"
     CDS             3567..3989
                     /locus_tag="LP452_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2015577.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3986..4216
                     /locus_tag="LP452_002935"
     CDS             3986..4216
                     /locus_tag="LP452_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2015578.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4714..4914
                     /locus_tag="LP452_002936"
     CDS             4714..4914
                     /locus_tag="LP452_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL2015579.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4941..6134
                     /locus_tag="LP452_002937"
     CDS             4941..6134
                     /locus_tag="LP452_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2015580.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(6202..7767)
                     /gene="guaA"
                     /locus_tag="LP452_002938"
     CDS             complement(6202..7767)
                     /gene="guaA"
                     /locus_tag="LP452_002938"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL2015581.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7826..8749)
                     /locus_tag="LP452_002939"
     CDS             complement(7826..8749)
                     /locus_tag="LP452_002939"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL2015582.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8945..9550
                     /locus_tag="LP452_002940"
     CDS             8945..9550
                     /locus_tag="LP452_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2015583.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9858..10874
                     /locus_tag="LP452_002941"
     CDS             9858..10874
                     /locus_tag="LP452_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2015584.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            11015..12316
                     /locus_tag="LP452_002942"
     CDS             11015..12316
                     /locus_tag="LP452_002942"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL2015585.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            12362..13024
                     /gene="deoC"
                     /locus_tag="LP452_002943"
     CDS             12362..13024
                     /gene="deoC"
                     /locus_tag="LP452_002943"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL2015586.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            13045..13443
                     /locus_tag="LP452_002944"
     CDS             13045..13443
                     /locus_tag="LP452_002944"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL2015587.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13509..14591
                     /locus_tag="LP452_002945"
     CDS             13509..14591
                     /locus_tag="LP452_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2015588.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14803..16365
                     /locus_tag="LP452_002946"
     CDS             14803..16365
                     /locus_tag="LP452_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2015589.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            16358..17491
                     /locus_tag="LP452_002947"
     CDS             16358..17491
                     /locus_tag="LP452_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015590.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVILLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17491..18447
                     /locus_tag="LP452_002948"
     CDS             17491..18447
                     /locus_tag="LP452_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2015591.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18546..19712
                     /gene="deoB"
                     /locus_tag="LP452_002949"
     CDS             18546..19712
                     /gene="deoB"
                     /locus_tag="LP452_002949"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL2015592.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19736..20554
                     /locus_tag="LP452_002950"
     CDS             19736..20554
                     /locus_tag="LP452_002950"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2015593.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20571..21278
                     /gene="deoD"
                     /locus_tag="LP452_002951"
     CDS             20571..21278
                     /gene="deoD"
                     /locus_tag="LP452_002951"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2015594.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKKFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6921 a         3800 c         4578 g         6155 t
ORIGIN      
        1 gttgatatta gaaaaacaag ataattggaa gattgtaaaa taacaaatat tggtacatga
       61 ttacagatac tttgtaatca tgtactcttt ttgataaaaa attggagatt cctttacaaa
      121 tatgctctta cgtgctatta tttaagtgac tatttaaaag gagttaataa atatgcggca
      181 aggtattctt aaataaactg tcaatttgat agcgggaaca aataattaga tgtccttttt
      241 taggagggct tagttttttg tacccagttt aagaatacct ttatcatgtg attctaaagt
      301 atccagagaa tatctgtatg ctttgtatac ctatggttat gcataaaaat cccagtgata
      361 aaagtattta tcactgggat ttttatgccc ttttgggttt ttgaatggag gaaaatcaca
      421 tgaaaattat taatattgga gttttagctc atgttgatgc aggaaaaact accttaacag
      481 aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa
      541 cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa
      601 cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt
      661 tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg
      721 caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga
      781 ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt
      841 atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt
      901 atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg
      961 gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg
     1021 aacaagagga aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg
     1081 caaaaagtaa tatagggatt gataacctta tagaagttat tactaataaa ttttattcat
     1141 caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa
     1201 aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta cgagattcgg
     1261 ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg
     1321 aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt
     1381 tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa
     1441 atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt
     1501 tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt
     1561 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
     1621 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
     1681 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
     1741 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
     1801 agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga
     1861 tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt aaaatctgtt
     1921 ttaagtacgg tttatactat agccctgtta gtactccagc agattttcgg atgcttactc
     1981 ctattgtact ggagcaagcc tttagaaaag ctggaacaga attgttagag ccatatctta
     2041 gttttaaagt ttatgcacca caggaatatc tttcacgggc atataacgat gctcccaaat
     2101 attgtgcaaa tatcgtaaat actcaactga aaaataatga ggtcattatt attggagaaa
     2161 ttcctgctcg atgtattcaa gattatcgca atgatttaac tttttttaca aatgggctta
     2221 gtgtttgttt agcagagcta aaaggatatc aggttaccac tggcgaacct gtttgccaga
     2281 cccgtcgtct aaatagtcgg atagataaag taagatatat gttcaataaa ataacttagt
     2341 gcgttttatg ttgttatata aatatggttt cttattaaat aagatgaaat attctttaat
     2401 atagatttga attaaagtgg aaaggaggag attgttatta taaactacaa gtggatattg
     2461 tgtcctattt gtggaaataa aacaagacta cgaatacgag tggatactat acttaaaaat
     2521 ttccctttat actgccccaa atgtaagaac gaaactttaa ttaatgttca aaaaatgaat
     2581 ataataacaa tcaaagagcc agacgccaag acgcagagcc gataatttga gaaatgaaac
     2641 tctcatctta tcggctcttt ttgtttatct gaattttact gactagcctt caatatttct
     2701 tgcttcattg ataccttttg ctagtgcttc cattaaggat agttctttgt ctgtaaagct
     2761 atccatgtat ttttctacct gtaaccttcg agtgctttta atcaaattat ctgtaggtaa
     2821 gaaaaattca tcaatagata catggagtaa tgatacaaga tcatacagca cttgtgtact
     2881 tgggtgttgt cctttgtttt ctatatttgt taaataacgt gggtcaattt caatcattgt
     2941 tcccacttgt tcacgagtga gaccccgttt cattcgagcc tctttaatcg ctaaaccaac
     3001 ggctctaaaa tcgtatttat cttctttttt acgcatacta aatcacctct atacatttta
     3061 ctgttcctat ataaccataa acaggtatag aaaaacatgt tatatagttt atgagttcct
     3121 atagaacact gtggaattta ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga
     3181 ttttatttac ctatacaact ttataaaatt tagtcatact cctagaaagt agataaaatt
     3241 ggcgacttaa tggagggaaa agtcgccatg agaaagataa tctgtcatct agttctctta
     3301 attaagttct tatcaaaaaa acttatccac ctgtactgga tagatatgac catcaatgtg
     3361 gttttgcaga ttacatagat agaaaaatca aagcatatga acagagaaaa caatctgttt
     3421 atgtgctttt ttggtcactt aataaatatc ttttaggatt tctcttcatt tatgcaacaa
     3481 atcccccttt cgtgtcgggg tttagtatga agggagataa agaagcacat gccactccct
     3541 ttcacgacag aaagggggtg aaaagcatga aaccatcttc ttttcaagaa accatagaaa
     3601 atcagtttga ctatatctgc aaacaagtta ttgaagatga acgaaaagat tacttcaaac
     3661 atctagacag actatcagca aaagaagtct cgttttctga tatagataac taccttgtca
     3721 actctttttc tactgttgat acgtacgtca ctgatttcca actatttaaa ttatttggat
     3781 taagaatcaa tgttgaaagt gatttattaa gtgaagccct gcgtcatctt tcagagaaaa
     3841 aacggaacat tattttactt cattacttta tggatatgag tgatgtggag attgcggagt
     3901 tactaaattt gaatcgttcg actgtttatc gtcatcgaat aagtggacta gccatgatta
     3961 aagaattcat gaaggagtgt aaagaatgaa aacagcttat ccaagggttc ccttccccct
     4021 cattgtaaag gcgacagatg gcgacgtgga agcaattaat cagattgtta aacattatcg
     4081 agggtatacc tcaaaacgtt cattacgtcg catgacagac gaatatggaa attctcatat
     4141 ggttattgat gaaaccctac gtggcagaat ggaaacgaaa ctcatctcta agattttatc
     4201 gttcgaaatt aaatagtccc attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc
     4261 cgatttggga ggtttgttat tccatcaaag catattgtgt gttcagtagg ttttgatagg
     4321 ctaacaagcc aaacgttctt tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga
     4381 aaagagctga tgaactaaca cgccatgacc tacaaaatta gtgagcgatt catgttagta
     4441 attcgaaagt aatctttagc aggattgctg ccatgacttt cttatcgtga taatgatact
     4501 cccgtatagc catagtccga gcgttagaag cgtcacaggc aatgggtacg gctacatgag
     4561 aaccatgcag aagtgaaatt cctgtgagct ttgctagagc tgttcgattg ctaagatatt
     4621 cttctcttgt cagtgtatta ttataaaact atttgatata attataggtg aatattggag
     4681 ttttcctagc ctataattgg gaaaggagta ataatgataa cggtcacaaa aaaggattta
     4741 gttgagctag gatatggaac ttctttttca gcagacatca taaaaaaagc aaaagaattg
     4801 atgattacaa aagggcatat ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa
     4861 gctgttgaag aaattctagg aattcggttt tcaaatgaaa acaggtctga gtagtttttg
     4921 cacagtaagg agtaaaaact atggttaaag acccaataaa aaaagcaaag aatggaacct
     4981 attattttcg tgcaaatcta ggttatgatt ctaaagggaa aaaaattcaa aaatatcgta
     5041 gtggattcac tactaaaaaa gaagcaagag aagaatattc gaaactgcta ttgatgaaac
     5101 cagaagaatt aagtgagaat aaggataaaa tgacgtttaa acattatatc tttgaaattt
     5161 tccttccatg gtataagaca caggtaaagc tcagaacata tgaaaatcgc ttaccaacta
     5221 ttaagaagca tttctcttat tttgataaaa tggctgtttc cgatattgag cctattgatg
     5281 tgcaaaattg gcaactaaaa ttatcaaaga agtgtaagtc ttcttatgta agagctgttc
     5341 aaggactatt ttcagttgct atggatcgag caattgtcct tgggataacc accacaaatc
     5401 catctaagat tattggcaat gtgaaaaagc aaaagtctaa aattgaattt tggacaaaag
     5461 aagaatttga aaaagtaatc tctctaattt acaaagaaga ttactatcaa cactttttat
     5521 ttatctctct gtggttttta tttatgactg gaatgcgaat tggtgaagct actgccattc
     5581 aatgggaaga tattgatttt gactcaggag tactaaccat caacaaaaca ctctattaca
     5641 agaatcaatc gaattataga tttgttgaac caaaaacaaa ggcgagcgtt cgtcatattg
     5701 tattagataa atgcacatta acttatttat ccgaatggaa ggaagtacaa caaaatctaa
     5761 ttcagacaga ctttgtaatg agctataacg gtattcctac ccaaaaacat acactatcat
     5821 atgctattga acgctatgct aagctggctg gtatccatcg aatcagaatc catgggttac
     5881 ggcattctca tgcttccttg ttaatcagta tgggagaaaa tccacttatc attaaagatc
     5941 gtctaggaca cgaagatatt gagacgactt taggaacgta tggacatctt tatcctaaca
     6001 gtaatttcga ggtagctaac aaattaaatg gagccatttc ctttaaagaa gcaaaacaaa
     6061 attatgatac ttcaccaaag aatcaattta ctgtagacta cttgcgaaac aaagaaatga
     6121 aaaaagtgca ataaaagtgc agtaaaaaga taagaagagc cagaaaccct tggtataaga
     6181 ggatttctag ctctcctaca actattccca ctccactgtt gctggtggtt tagaagtgat
     6241 gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg
     6301 caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc
     6361 acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat
     6421 ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc
     6481 ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc
     6541 aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg
     6601 catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc
     6661 gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt
     6721 ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa
     6781 agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc
     6841 attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa
     6901 gcgatctctt gcatcgactt tgatgatgtt caagccgaat tttccgccta gactttccat
     6961 tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag atgtcaattg
     7021 gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag
     7081 accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat
     7141 aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag
     7201 caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt
     7261 acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa
     7321 gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg
     7381 tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc
     7441 gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata
     7501 gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt
     7561 ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt
     7621 tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat
     7681 acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac
     7741 gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc
     7801 aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat
     7861 gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct
     7921 gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac
     7981 gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta
     8041 acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga
     8101 cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat
     8161 tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga
     8221 tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac
     8281 ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca
     8341 tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct
     8401 gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac
     8461 tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt
     8521 tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa
     8581 tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca
     8641 aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt
     8701 ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct
     8761 gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag
     8821 agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg
     8881 acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga
     8941 agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg
     9001 ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg
     9061 tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc
     9121 tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc
     9181 atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc
     9241 gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta
     9301 tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg
     9361 aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac
     9421 actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga
     9481 aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt
     9541 gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt
     9601 acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt
     9661 cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac
     9721 agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata
     9781 ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat
     9841 agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat
     9901 cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa
     9961 gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa
    10021 atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct
    10081 tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca
    10141 tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc
    10201 accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat
    10261 tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc
    10321 gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt
    10381 ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat
    10441 tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg
    10501 ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat
    10561 tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat
    10621 gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat
    10681 tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa
    10741 gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa
    10801 gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat
    10861 tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt
    10921 gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg
    10981 tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa
    11041 aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac
    11101 gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga
    11161 catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat
    11221 cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga
    11281 cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat
    11341 gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg
    11401 atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc
    11461 ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga
    11521 cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat
    11581 cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa
    11641 gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa
    11701 ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa
    11761 tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt
    11821 agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac
    11881 cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt
    11941 caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat
    12001 tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat
    12061 cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga
    12121 agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac
    12181 aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga
    12241 attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga
    12301 catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga
    12361 aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc
    12421 tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc
    12481 agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt
    12541 aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc
    12601 catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa
    12661 ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc
    12721 gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg
    12781 cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg
    12841 cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt
    12901 caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc
    12961 tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta
    13021 ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct
    13081 attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc
    13141 gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta
    13201 acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt
    13261 acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga
    13321 caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt
    13381 gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta
    13441 taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct
    13501 ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt
    13561 tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat
    13621 cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca
    13681 accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa
    13741 atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc
    13801 aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga
    13861 tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg
    13921 atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag
    13981 gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag
    14041 gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag
    14101 aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata
    14161 aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt
    14221 caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca
    14281 aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa
    14341 acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag
    14401 aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg
    14461 gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag
    14521 cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg
    14581 aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt
    14641 gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac
    14701 aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc
    14761 aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt
    14821 gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc
    14881 aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa
    14941 tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg
    15001 cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg
    15061 gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga
    15121 agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa
    15181 gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt
    15241 ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt
    15301 tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg
    15361 aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc
    15421 aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac
    15481 gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc
    15541 aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg
    15601 gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc
    15661 gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg
    15721 actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat
    15781 aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa
    15841 tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa
    15901 aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa
    15961 ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca
    16021 ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg
    16081 attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt
    16141 ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa
    16201 attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt
    16261 gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa
    16321 gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt
    16381 ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt
    16441 gatggctgct tttggttttc agcccatcca aggttattct tctatgctag atgcagccct
    16501 tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc
    16561 attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc
    16621 actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt
    16681 tattttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt
    16741 tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa
    16801 ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg
    16861 ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt
    16921 aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct
    16981 tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg
    17041 attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc
    17101 gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta
    17161 tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc
    17221 cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt
    17281 gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt
    17341 atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc
    17401 taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac
    17461 gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc
    17521 cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt
    17581 tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt
    17641 agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc
    17701 gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca
    17761 gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct
    17821 ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag
    17881 aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc
    17941 tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg
    18001 atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct
    18061 gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc
    18121 ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct
    18181 ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat
    18241 cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt
    18301 ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat
    18361 ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac
    18421 gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga
    18481 agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa
    18541 taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac
    18601 cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag
    18661 caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag
    18721 gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag
    18781 ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc
    18841 gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc
    18901 gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac
    18961 accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg
    19021 cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg
    19081 atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg
    19141 aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac
    19201 acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca
    19261 acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg
    19321 gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt
    19381 tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga
    19441 ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc
    19501 taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag
    19561 aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag
    19621 gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa
    19681 atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac
    19741 caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga
    19801 aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga
    19861 tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca
    19921 tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag
    19981 attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc
    20041 cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt
    20101 tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt
    20161 gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac
    20221 gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga
    20281 aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc
    20341 tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc
    20401 tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca
    20461 agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa
    20521 agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc
    20581 atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc
    20641 gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg
    20701 gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa
    20761 tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga
    20821 aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag
    20881 tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaaattc caagcctttc
    20941 attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa
    21001 agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt
    21061 tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg
    21121 ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg
    21181 accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa
    21241 tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc
    21301 tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg
    21361 acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg
    21421 tttaactctg agtcacaagg aacaatttca aaaa
//
LOCUS       DAFELO010000202        18009 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.055, whole genome shotgun sequence.
ACCESSION   DAFELO010000202
VERSION     DAFELO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.055"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..324
                     /locus_tag="LP452_002952"
     CDS             <1..324
                     /locus_tag="LP452_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL2015595.1"
                     /translation="SRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRP
                     ENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLS
                     SVENK"
     gene            complement(474..1415)
                     /locus_tag="LP452_002953"
     CDS             complement(474..1415)
                     /locus_tag="LP452_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2015596.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1541..2662
                     /locus_tag="LP452_002954"
     CDS             1541..2662
                     /locus_tag="LP452_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2015597.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2920..4422
                     /gene="eat(A)"
                     /locus_tag="LP452_002955"
     CDS             2920..4422
                     /gene="eat(A)"
                     /locus_tag="LP452_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL2015598.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4450..5016
                     /locus_tag="LP452_002956"
     CDS             4450..5016
                     /locus_tag="LP452_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2015599.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5227..6288
                     /locus_tag="LP452_002957"
     CDS             5227..6288
                     /locus_tag="LP452_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2015600.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6291..7187
                     /gene="murQ"
                     /locus_tag="LP452_002958"
     CDS             6291..7187
                     /gene="murQ"
                     /locus_tag="LP452_002958"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL2015601.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7201..9183
                     /locus_tag="LP452_002959"
     CDS             7201..9183
                     /locus_tag="LP452_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2015602.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9248..9694)
                     /gene="nrdI"
                     /locus_tag="LP452_002960"
     CDS             complement(9248..9694)
                     /gene="nrdI"
                     /locus_tag="LP452_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2015603.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            9905..11329
                     /locus_tag="LP452_002961"
     CDS             9905..11329
                     /locus_tag="LP452_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2015604.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11510..12283)
                     /locus_tag="LP452_002962"
     CDS             complement(11510..12283)
                     /locus_tag="LP452_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2015605.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12280..13185)
                     /locus_tag="LP452_002963"
     CDS             complement(12280..13185)
                     /locus_tag="LP452_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2015606.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13339..13659)
                     /locus_tag="LP452_002964"
     CDS             complement(13339..13659)
                     /locus_tag="LP452_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL2015607.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14133..14819
                     /locus_tag="LP452_002965"
     CDS             14133..14819
                     /locus_tag="LP452_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL2015608.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            14821..15765
                     /locus_tag="LP452_002966"
     CDS             14821..15765
                     /locus_tag="LP452_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL2015609.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            15964..16608
                     /gene="pcp"
                     /locus_tag="LP452_002967"
     CDS             15964..16608
                     /gene="pcp"
                     /locus_tag="LP452_002967"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL2015610.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            16687..17964
                     /locus_tag="LP452_002968"
     CDS             16687..17964
                     /locus_tag="LP452_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2015611.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5752 a         3195 c         3847 g         5215 t
ORIGIN      
        1 tcgcgcggga ttctggaact gttgcgggag ggtcagcaag gagtgacttt gcggacattt
       61 gaatccatgt attttcaaaa gaaactagtg acggataatc aagcgattgc tcattatcat
      121 ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg atttagatga attaccggaa
      181 tttctgcata caccatttaa gccaatccta tcagagctat tggaatttta tgatgcagaa
      241 aattgggcaa aacgatttat cactaacgat tccactattt atgagcaata cgaatatgac
      301 ctctcctctg ttgaaaacaa ataaaaaacc tacattttga agagattatg tactgaaaca
      361 aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc ttttattggg
      421 cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc cttttattct
      481 gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca atatggtgta
      541 gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc gatttcagtc
      601 aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc gatgttgtca
      661 ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat gattccttct
      721 tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc tgccgctttt
      781 tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt caatgttcca
      841 tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat cggatttaaa
      901 tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc tggttccaag
      961 ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc agctagacca
     1021 gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt ttccagtaca
     1081 tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt aataatggat
     1141 tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac gatcaagtcc
     1201 acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt tgcgaccact
     1261 tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc
     1321 caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc gattcggctg
     1381 cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt tattcaatga
     1441 gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg
     1501 aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt tggcggcaat
     1561 tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc taaatggctg
     1621 ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag aaaatactgc
     1681 agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga tggatctctt
     1741 attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa acaccgcagt
     1801 catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg atcaaactat
     1861 ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc ttcaagaagt
     1921 agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc taggattact
     1981 ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat tatctactaa
     2041 aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc
     2101 agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg gaagacaaaa
     2161 aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga ttgctcagca
     2221 aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag cagcaggcgg
     2281 tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg atttagtcag
     2341 ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta caggagaagg
     2401 gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag tgcttgcaaa
     2461 aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc tagaaggact
     2521 ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt tagaagaagc
     2581 aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac ttagcagatt
     2641 tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc gtaatttcaa
     2701 atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca
     2761 ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga ttggcttggt
     2821 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
     2881 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
     2941 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
     3001 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
     3061 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
     3121 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
     3181 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
     3241 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
     3301 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
     3361 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
     3421 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
     3481 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
     3541 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
     3601 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
     3661 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
     3721 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
     3781 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     3841 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     3901 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     3961 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     4021 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     4081 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     4141 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     4201 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     4261 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     4321 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     4381 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     4441 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     4501 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     4561 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     4621 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     4681 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     4741 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     4801 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     4861 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     4921 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     4981 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     5041 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     5101 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     5161 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     5221 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     5281 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     5341 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     5401 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     5461 ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat ggattatcat
     5521 atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt gaatgctagc
     5581 acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt ctcacagatt
     5641 gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga atggtatcag
     5701 aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt accaggagat
     5761 acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca tcgagggatc
     5821 catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt ttacatcgga
     5881 gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa agaacagaca
     5941 gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt agggaagcat
     6001 acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg ttttcgagag
     6061 attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat tactttagat
     6121 aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga tttgccagcg
     6181 gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt gatcaaccaa
     6241 atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa atgagcaaaa
     6301 tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga ttagatgaga
     6361 tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg gccgctgcgg
     6421 ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag tcctttaaaa
     6481 aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt gtgttagatg
     6541 ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt ctaattgctg
     6601 gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat gagctagggc
     6661 aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt atcgcagcta
     6721 gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc ggtgctgtca
     6781 caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat ttcccaatcg
     6841 aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca ggaacagcac
     6901 aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt aaagtattca
     6961 ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa
     7021 tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt gaagaagcag
     7081 atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa gaggaagctc
     7141 aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg ggaaaagacc
     7201 atggcaaaaa acgaactgac tttggaagaa ctggcaaaac aaatttataa aggggcaggc
     7261 ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat gaagatcatc
     7321 aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct tggtgtagta
     7381 gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt agcacagcaa
     7441 atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag tacagctaca
     7501 gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg
     7561 gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga catttctaat
     7621 atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg gattgccgca
     7681 gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca acaatatatc
     7741 gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta tacaggtatc
     7801 aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg ggccgttgtg
     7861 atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg tgaagctctt
     7921 gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt
     7981 gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac accaactctt
     8041 tcgttgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc aggtttcatt
     8101 tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc atttgccggc
     8161 ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca aattttgact
     8221 ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg
     8281 atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg caaaaaagat
     8341 aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg aatcggcgaa
     8401 ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc ttgtatcggt
     8461 ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat cgctattgga
     8521 ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg ctacatcctt
     8581 ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg tattcctaaa
     8641 gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga agtgcctgaa
     8701 aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt tgctgacgga
     8761 acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat gatgggtgaa
     8821 ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg ggtagtcagc
     8881 tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt agaaatcttg
     8941 cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt gaaagtaaca
     9001 gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga agcgatcaaa
     9061 gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg tgtaaaaaac
     9121 tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga tgtcaaagct
     9181 taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt cagcttcttc
     9241 ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca tcagatggtg
     9301 ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca tattgtttgg
     9361 ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca ccaagacaaa
     9421 gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag atttctgtat
     9481 caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta aaaaatggtt
     9541 ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact tcaggattga
     9601 caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca aaggcacgcg
     9661 tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt ctttcctttt
     9721 agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact aggcgctttg
     9781 cttgtataat gagaaaaaag ccggtagaat tatatttcta ttaaagtttt tattaattaa
     9841 gtaaaaataa aaaaggtttg ttatactttt tgataaaagc ggttttattt tggaggatgc
     9901 ttacatggat acagagcaac agaaaaaagg aattggttta attccacttg ctgccttagt
     9961 aattggttca gcgattggag gtggcgtatt tggtattatg acggacatct ccggatcggc
    10021 tggcggccca gctattttca gttgggtact cgtaggaaca gcaatgttga tgctctcctt
    10081 gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa ggcgggatct ttagttacgc
    10141 agaagaaggt tttggccgtt tcgctggttt tatcagcggt tggggttact ggctcacatg
    10201 ctggttaggc aacgtagctt ttgcaacatt gttgatgagc gcattaggat atttcttccc
    10261 agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg agtacagtct ttttatgggg
    10321 atatgcatgg ctagtgaatc ggggagtcga aaatgctagt ttcgtcaatg ctatcgtcac
    10381 attctgtaaa ttagtgccat tatttttatt tattgtagta gctatcagca cgttttcgat
    10441 ccaacaattc attgataact tttctagcga cattatattg aatgcgaatg gacagccgat
    10501 cccgttttcc agtcaagtat tgagttgcat catggtgatg ctttgggtat ttgttggtat
    10561 tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa tcagatgttg ggaaagcaac
    10621 gattctaggg atattcggct tgattattat ttacgtcttg gtttccatgc ttccatatgg
    10681 gacaatgacg atgaatcagc tgcaaacgat caaggcgcag cctgctatgg gctatgtgtt
    10741 tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac ggcggtttga tcatttcttt
    10801 agttggtgtt tggctctcat ggacgatctt accgattgaa acaatgggaa acatggctga
    10861 agacggactt ttgcctaaag catggggaaa actcaacaaa aatggggcac caacttatgc
    10921 tatcgtattg acgacgttat gtacgaatgt ctttttactt tcattattat tcactgacag
    10981 tgcttataac tttgcttaca cacttggtac agcagctatt ttcttcacgt ggcttttctt
    11041 aggattgtac caaatgaaac tttcttatca gcggaaagaa tgggtccaat ttttagtagg
    11101 gcttttagca agtgcctttc aagtatgggc tatgctagtc gtagccttta atgaagtaat
    11161 gcttgttctt gttcttttct tgccaggcat ttatttttac ctcaaagcac gtaaggagca
    11221 aaacgagcct gtacaacata tccacttaga taagatttta cttataggtg tgacgatggt
    11281 cggagtgatt gcaatggttt tatttgccac tgggatgctt cacgtataga agtataaaag
    11341 aagtataaag agaggttgtg agcctgtcgt ttattcaaaa acatactagg aaaggagctg
    11401 tgacacaaat gttgtcacag ctcctttttc agaataaacg gtgtttaaga gacagccaat
    11461 cggcaataag gctgaacgtc tctttcacag cctctttttt gatgagtcat catacatttt
    11521 ttcgcttaaa tacgagatat cctaatgcta agaacaaaac gatgtaagct aaattaccga
    11581 atagcatttg attagtagaa agatgagtca attgcttcat gatctcttcg tttccaattt
    11641 ggttttgcag attcaacata ctgatcggat tccatttgat ccactcccat ttttggatga
    11701 gtgcaataag gataccagat atgattgacg aagcaaaata aaagacgatt cctgctgaga
    11761 tggatgcgcc ggaactattg ataaagcaag caatcatcaa cactaaactt aaaatcagcc
    11821 ataaaccgat ataactaccc aaagtataag tgaacaagct ttggatcagt gtttgaccag
    11881 tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat cagtttcatc aacacagtga
    11941 caataatagc taaaaggtat aaataaacag aataaataac taatgtgatc catttgctta
    12001 ctaatatttc tcctcgagaa tactttcgat aaaggagatt tttcaatgta ccatattgtg
    12061 cttccatcga aatgatcgtg cttgcagctg caatcatgat aaagacaatc caagatgtgg
    12121 cactaaaaag ctgagcagtc atttcaactg gatcaatgat cgtcgtgtat tttttggcta
    12181 gaagtgctgt tcctatgagc aaaacaacta atagaacaga taaaacagca gttgactttt
    12241 tcttgatcaa tttaaataat tcttgtttta caagcaatgt catttttctt cttccccctt
    12301 tactgaatcc agaatttcca atagagaaga ttccaaatca ttcgtcagat gctggacgtc
    12361 tgtcacatgg atattttgtg cagacaatag ctggatcacg gttcctaata attcttcgtt
    12421 ttcttttaaa agcttgattt tatcaggatc ttccaaagta tatccagctt ctgtcaagat
    12481 cgttcgagca gctagatcat ttgttgtaga gataacgatg aaatcttgcg ctgtttggtt
    12541 caattcagcc attgtacatt gtttgatgat ctttccttga ttgatcagga tcagctcatc
    12601 gaccattttt tcaagctcgc tgagtaagtg actggagatc aagaaagagg tgccctgagt
    12661 agccaagtcg gtaatgattt ttcgcacatc tcttgttgct tgtggatcca aaccgttcat
    12721 cggctcatct aaaataacga tagacggatg gttcagtaag gcaagagcaa ttcccaattt
    12781 ttgtttcatt cccaacgagt aggatttcgc ttttttgtga atgtactttt ccatcttgag
    12841 ctgattcacg atagtgtcga tagcagatac atcgttggtt tcagagaata acttcagatg
    12901 atcatagcct gttaagaatg gatagatacc cgggtattcg atcaatgctc ctacttgttc
    12961 caacccttta tgactagagg gagaaatctc attttcacca atacagattg ttccatctga
    13021 gtaatggatc agtcctaaaa tagctttcat gatcgttgtt tttccagcgc cattaggacc
    13081 tactaatcca acgattttgc caggggataa gctaaaactg acatcgctga gggcttgttt
    13141 gtgaccaaag ttcttggata attcttggac cgaaagactt ttcaaaaata ttcactcctt
    13201 ttctatagat agtacaagca tacacctgat gagtggagaa gtcgctaggt cattggactt
    13261 atttcttatt ttttcataaa aaaattgaat attaaataaa aaggcttatt ttttctgatc
    13321 agtacttatt ccaagtgtct agtggccgct agtaatcttg atgccaatga tactgatgag
    13381 tagcatcccc acaaagaacc aagtggaaag attcagctta tcatgaaaca agaccacacc
    13441 aattatgacc gaaccaactg cgccaatccc tgtccagata ggataagaga gactcatcgg
    13501 caaacttttg attgcctttg acaaaaagta aaaactagca atcatgccaa tcactgtgta
    13561 aaaagaataa ttgattttag aaaaaccatc actccatttc atcatcgttg accagaatac
    13621 ttccaaaatt cctgctatga ttaattccat ccatgccata cttacattcc tccttaacta
    13681 taaaaaaata cacaaaaaga ggaaaatcat cccttcaaag acctaatgga atgatcatcc
    13741 tctttatcct acccggtggc agattgtata tctttttctg tagtatagca cacctatcca
    13801 gatatcggca atctcatact ttttagtaaa gtgtaccaag cactgacgga tatatcgtta
    13861 gcggaaaaga agatgaatgc tttgcaattt catagaaaga agttgttatt tattaatcat
    13921 ttaaaaaaaa cagtaactag accttatttc tatatattat cgggcaataa attaaaaaaa
    13981 gctttacctt acaataagta agataatttt tgtgataaaa aaataacgaa aaatatttaa
    14041 aaatagtgtc tatttcgtga gaaaacaact atatttttta tgtgttttgc tttataatta
    14101 aaaaaatatt gtcattttat ggaggaaaat atatggaaaa ttatcttagc cttttaggcg
    14161 tcttgattgt tattctcggg tttgcattta aattagactc gatattgatc gtaatggttg
    14221 ctttagttgt tacagctttg actggcggtc ttggcattca aggaatgtta gaacttttag
    14281 gtacaagttt tgtaaacaat cgcgggatgg cgatttttat catcattatg ttggcaacag
    14341 gaacgcttga aagaaatggt ctgaaagaat cagcggcttc cttgatcaag cgattcaaga
    14401 aagtatctgc tggtatgatt gtagatattt atgggatctt tcgaatgatc tttgcggctt
    14461 ttaacgtgag ttttggtgga gttgctggat ttgttcgtcc aatcatttta ccgatggcat
    14521 tggggaccat cgaatcaaaa gggttagaag tccatcctaa gcatgaagaa gaactaaaag
    14581 gtatggcttc agctatggaa aatgtctgct ggttctttgg ccaagtacta ttcatcggag
    14641 gagcaggggc gttgctagtc caatcaacac tgaaagatct aggatatgac gtaacattgg
    14701 gacagcttgc tttagtggaa gttccagtgg ctatcacggc tttagttgtg gcaagtgtct
    14761 acttctatct taaagaaaag aaattagcga agaaatatta tccaggaaag gggcaataag
    14821 atggactttt ttacaaatcc tgatatttta ttaggcgata aattactgga gatcctttat
    14881 atcgtgatgg gactcgtatt gatttacact ggagtaagaa atttaacaga taaaaccaat
    14941 cctcatcgtt atgggtcagc ctttttctgg acggtcttag gggtagttat tgcaggagga
    15001 agatggcttc cggcaatcgt gaacggtggc ttgatctttg cgatgaccat accagcaatt
    15061 gctaagaaag tcagcaaagg agaaagccgg cttccatcaa aagaatatat ggaaaaaatg
    15121 tcagacaaac taggaatgaa aatttttatt ccagctttga gtatcggtgt ctttgcgatc
    15181 ttgtttgctc tatttaccaa tttaggtgct ttagtcggcg tgggcgtcgg tgtttttgca
    15241 gccatgctaa tcttgatgtt cttttctcga gataatcgac caagtacatt tttagatgat
    15301 gcagcagata tgctgggcac agtagggccg ttaagcatgt tgccaatgct tttagcttct
    15361 ctaggtgcgg tatttacaag cgctggagtt gggacagtga tttccaatgc agtaggtacg
    15421 atcgttccag aaggaaatgt gaacatcggc atcatcattt acgctcttgg catggtgata
    15481 tttacggtaa tcatggggaa tgcatttgcg gccatcaccg tgatgacggt tgggatcggt
    15541 gcaccattcg tatttagtca tggagcgaat ccagcgttaa taggaatggt tgcattgact
    15601 tgcggttttt gtgggaccct gctgacaccg atggctgcta acttcaatat cgtgcctgta
    15661 gcgatgctgg agatgaaaga caaatacgga gtgatcaaaa accagttgtt tattgcgtta
    15721 tttatgctag ttttccaaat tgcttatatg attctattta aataatagaa gagaggggtt
    15781 gtgacagaat aattgtcgca acctcttcta acattgtagt ggaacactat ttttttataa
    15841 gaatatatta agtttgatct gttatgttat ttaaactatt gtttccttta tttacgctgt
    15901 tcaaggaaaa tggtatgata aaagataacc atatgttaaa tcaatcggac ggaggaacaa
    15961 cagatgaaag tactagtaac aggttttgat ccatttggtg gggacaaagt gaatccagcc
    16021 tatgaagcag taaaaaaaat gccagatgaa atcagcggtg ctgaaatcat caaagtggaa
    16081 gtaccgaccg tattcgaaaa aagcagtcaa gtagtcaaag aagccatcca acagcaccaa
    16141 ccagatgtcg tgatctgtgt cggacaagca ggtgggcggt cagcagtctc gttcgaacgt
    16201 gtcgcaatca atctagcaga agcaagaatc cctgataatg aaggaaatca gccattcgat
    16261 acggctttag aagaaaacgg tccagccgct tactttacct cattgccaat caaagcaatg
    16321 acaaaaaatg tacaagatca tggtctacca gcgtacatct cttatacagc aggaacattt
    16381 gtctgcaatg atatcatgta tcgtttgctt catttgatcg aaacacaatt cccggcaatt
    16441 cgaggtgggt tcatccacgt gccattctca cccgatcaag tgatcgatcg tccagtgggt
    16501 acgccttcta tgtctttaga agacattgct tcttctttga cttatgcagt tgaagcagca
    16561 gtagaaaata aagaagatat ttccggaaat gccggaacaa ctcactaatt tagcaagaaa
    16621 cactggctgg ttgctaacgt tatgttagta gccagctttt tgtaattaga aaggaaagaa
    16681 acagccgtgg aagaatggca atccgtcttt gaagaatggt ttccaaaaga aatcagcaaa
    16741 agctatccca tcaaaatttc taaacaatac acaagcagtc aacgttggga gatctatgca
    16801 aaattaacga aaaaacagcg ggaactagtc gataagcacc gccgatatct aatcagctct
    16861 cgttttatgg aagaacatta tttagctgca acagattggg tgttttctga cttcaagatc
    16921 aatccgtttt ttcgaacaaa acgaagccag caaaaattgt attgtgagtg tggtagagaa
    16981 ttaaaagtgc aatacatcgt taaatcgcca aaaaccggaa agatcctaaa actaggcatc
    17041 aaccactttg ccgatcacct gcatgtctct ccgacagtgg cagcttcgat ccatcaggga
    17101 atgacaaaag ttgatctggc cttagacgaa ttattatggc ttaagcagaa gaatatcgat
    17161 tttcctgaag gactatggca aaaatactgt tttgtcttgt accaaaatcg tcggatgaaa
    17221 cagccatatt taccggatat aaaattagct cagcggctcg cagaatttcg ccaagtagaa
    17281 atgccgattt acatagcgga ttatcaagca ttggaaaatg aaatcaagaa aataagtgaa
    17341 cacatcaatg ggcagtcaaa aaagcgacag ataaaaaaag aactatttga cgactttgca
    17401 gaagaattag taaaggatgt agaagaattc ttgatcaact atcgcgcatt tttacgtaaa
    17461 gattggcaat ccatcgtata tgaagaagtg ccagtacatc ctaatgctta ttttgaaaca
    17521 tttatttccg tgttgagaaa gacaaaacgt caacgaacac cagaagtcac tgctcagatg
    17581 gaatattttg cgaaaaacca acgattcatc caaccaaaaa tctatctatt tatttggaag
    17641 caatattgtc actatggttt tacagaaggg ttcttcgaca gcattcctcg gatagtcaga
    17701 aatggttttt taaaagtatt gcgtaaagaa cgagaagcta tacaatctgc tgataagaaa
    17761 gaccgtacag tctcaaaaga aaaatggcaa ctagtagtga aagatatcca atctggaaat
    17821 gtgcaagaga caatcgacaa atggaaagga aaacattatc gttttactga agctcagaaa
    17881 caagcgttgg aatattatca gaagttagaa gaatcactgc gtttcaatga tgaggcaaga
    17941 aagtatctga aggaattact ataaaaaata gaggctggga cagaagcgtt taactccaag
    18001 aaataagaa
//
LOCUS       DAFELO010000203        14956 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.066, whole genome shotgun sequence.
ACCESSION   DAFELO010000203
VERSION     DAFELO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.066"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            complement(255..722)
                     /locus_tag="LP452_002969"
     CDS             complement(255..722)
                     /locus_tag="LP452_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL2015612.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            845..1696
                     /locus_tag="LP452_002970"
     CDS             845..1696
                     /locus_tag="LP452_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2015613.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(1947..3905)
                     /locus_tag="LP452_002971"
     CDS             complement(1947..3905)
                     /locus_tag="LP452_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2015614.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3902..4774)
                     /locus_tag="LP452_002972"
     CDS             complement(3902..4774)
                     /locus_tag="LP452_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2015615.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(5116..5988)
                     /locus_tag="LP452_002973"
     CDS             complement(5116..5988)
                     /locus_tag="LP452_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2015616.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            6116..6685
                     /locus_tag="LP452_002974"
     CDS             6116..6685
                     /locus_tag="LP452_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL2015617.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            6752..7489
                     /locus_tag="LP452_002975"
     CDS             6752..7489
                     /locus_tag="LP452_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2015618.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            7720..8988
                     /locus_tag="LP452_002976"
     CDS             7720..8988
                     /locus_tag="LP452_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2015619.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            9475..9939
                     /locus_tag="LP452_002977"
     CDS             9475..9939
                     /locus_tag="LP452_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2015620.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            9923..10552
                     /locus_tag="LP452_002978"
     CDS             9923..10552
                     /locus_tag="LP452_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2015621.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            10906..12384
                     /gene="msr(C)"
                     /locus_tag="LP452_002979"
     CDS             10906..12384
                     /gene="msr(C)"
                     /locus_tag="LP452_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL2015622.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            12431..12877
                     /locus_tag="LP452_002980"
     CDS             12431..12877
                     /locus_tag="LP452_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL2015623.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(13076..13591)
                     /locus_tag="LP452_002981"
     CDS             complement(13076..13591)
                     /locus_tag="LP452_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2015624.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            13708..14910
                     /locus_tag="LP452_002982"
     CDS             13708..14910
                     /locus_tag="LP452_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2015625.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4675 a         2711 c         3079 g         4491 t
ORIGIN      
        1 caaaaccgga aggttaaaac cctaactcta atatacaagg aaaaatggga aatagctttc
       61 catattttgc atttttcaga actaatgccg agggtcagct acttgaacac cgtttatcaa
      121 gacgtcatga cttcagcgct ttgacctgag cattagagag gaaaaagggg catgacaagg
      181 cttttgtccc ccggagaata aaagaattta tgtagacgat tccgatcaga aaaatgcagg
      241 cacagtagag agaattataa aaatctctct acgaggcctg tgttttctgg tcttttttct
      301 ttttccaaaa cggctatttt atgtatcaga ctatcaagtg tgatcttagc catttcttct
      361 tctgcctttt tctgcagccg agcgtagctt tcttctaaag cttcttggat attcgcacca
      421 acgacgcaat ttggatttgt ttttggatcg acttggataa gctgtgtatt accttcgatg
      481 ctgcgataga catctagtaa agaaatctga tctggacttt tggcaaggga aggctgcggt
      541 tttccggttt ttgtaaagat caaaccggat tttttgagct gactcatgat tttacgtaca
      601 tttgctgcat ttgtttcgat actactggca atcatctcac tagaaagtga tcgggtatct
      661 tgaaacattt caatataggc aagtacatgg attgcatcac taaattgcag agaatacttc
      721 atcagttgtc atcctttcat tttttttgct tgacttatct taccacactc tttatgataa
      781 aggtgtaatt tataaatgta cagctgtaaa tcttaacaaa taaaaagaat agggagtggc
      841 aaatatgcat tatttagtaa caggagcaac tggaggtttt ggtggttatg cgctggaatt
      901 cttaaaaaag atggttccac aaaatgaaat ctatgtacta gtacgtagtg aagaaaaagg
      961 agaagcactg aaagaagctg gatttaatat acgtattggt gattatgatg atttagactc
     1021 aatgaaagaa gcgttgaaag gaatagatcg attgcttttt gtctctggcg ttccaggaaa
     1081 tcgtcaagcc gaacataaga atgtagtgaa ggcagcacaa gcagctgggg tatcttatat
     1141 cgcttataca agttttgcag atgccgatca ctcgatcagt gtattagcgc cagatcacca
     1201 attcactgaa aaaatgatta aggattcagg tatttcccat acatttttac ggaataattg
     1261 gtatgtagag aatgaattac ctatcattgg gcaggcttta aaaactggtc aattcgttta
     1321 tgctgcagga aatggaaaaa caggttgggc attgaaacgt gaatatgcag aagctgcagc
     1381 aaaagtacta gtagaaagtg actctccaga aatcttagaa ttatccggca atctaactga
     1441 ctatgaagaa ttagccaagg cattagagcg tgcgacaggt aaagagttag aaattattga
     1501 agcaagtgat gcagcgtttg tcgaaaatct aaaagaagcc ggattccctc aagaagcagc
     1561 tgacatgttt ttagcgatcc aacatgatat taaaaaggat cagttagcta tttcctctga
     1621 tgatttagaa aaagtattgg gaaaaccatt aacttctctt gaagcagcat taaaagaaat
     1681 attggatcag gaataatgta taaagaggtt gtgaaaaagc cgattagttt tgagtatgaa
     1741 gaaaaatttt gtgaaaaagc tgttctgcat caagtaataa gaagagtcaa ataaaaaata
     1801 gctcttcata tgtttcgttt agctggactt attaccgcgg agttggcagg agaacgccgt
     1861 ttttattcgg aaaaaggagc tgagacaaga tttttgtctc agctcctgtt tgtttatcga
     1921 taaaaaagtc tggggaagac gatggctcag gctttgttgg aaacagagcc gattttatca
     1981 tttgctttta catcacgtgc gggttcgatt tctaagcttt ggactttgtc gccatttgtg
     2041 aatacaacga ccatatccgt gtttttgcct gcagattgaa tagcggcaag atcaactaaa
     2101 gcaatcaatt gacctcttgc gactttttga ccttcttcta catataaagt gaatggttcg
     2161 ccttttaagt caacagtgtt gatgcccata tgaagcaaga cttcgatacc agcatctgtt
     2221 tggattccga cagcatgttt cgttgggaaa atgtttgtga tcgtaccagc gattggagag
     2281 aagatttctc cattttctgg taatacagca tagccgtcac ccatcatttt ttccgcgaat
     2341 acatcatctg aaacttcagt gattggaatc agtttcccat ttgcgatagc gtaaatatct
     2401 tgttcgatcg tttcaccggc aaatgtttct gttttaacca cggtgtttgt ttgtgcaggt
     2461 cgtgctgcag caccaccatg tagttttgcc atctcatctg caacgaattg tacttctgta
     2521 cctacgatga cttgcgcatt ttttccgtcg atgatcttca taccaggtac gccagtggct
     2581 ttgattttat tttgatcgat caggcctgta tctttgactt gcaaacgaag gcgtgtagta
     2641 cagttatcga tcgaagttac attgtcggct ccgcctaatg cagcgtagat tttgcttgct
     2701 tgtgccgcat atttattgtc tgaattagtc gttgcagtat cgtcatctga aagatctgct
     2761 tcctcgcggc caggagtcat cagatggaat tttttgatag caaagttaaa gccaaagtag
     2821 taaatcactg ctgttactaa tccttggacg atcaacatca atggctgatt cgcaatcgga
     2881 tttttcaaag ataggaagta atcgacaaat ccggcactaa aggcaaatcc tgctgtccaa
     2941 tggaagaatg cactcactgc taaagaaatc ccagtgaaaa ttgcatgcaa gacatataat
     3001 ggccaagcaa cgaacataaa tgaaaattca agtggttctg tcacaccagt aaagaatgaa
     3061 gcaaaagctg ctgctagcat caaggaagct gtttttgctt tgttttctgg acgtgcattt
     3121 ctgtaaatag catatgctcc tgctggaagc ccgaacatca tgattgggaa aaatccagct
     3181 tgatacattc ctgttacgcc ttttgttcct tcagaagcca aaaatttacc aatatcattg
     3241 attcctgcta cgtcaaacca gaacacggag ttcattgctt gatgcaatcc tgtagggatc
     3301 agtaaacggt tgaagaagcc ataaagacct gcaccacccc agcctaaact caaaatgaat
     3361 ttagcaaaag aaactaaagc agagtaaacc ggaggccata cgagcagtag cactgcactg
     3421 atcagcaaca tgacaaaggc agccaagatt ggcactagac gcttgccgct aaagaaagat
     3481 aaagccatcg gtaattttgc ttcgtggaaa cgattataaa gagctgccgc aatcaatccg
     3541 gcaataatac caataaatac gttactgatc tgagcaaaag atggatcaac tgattctggt
     3601 ttgatattca ataaagtagc tactgaatca gtttttaaaa catttactgg aacttcaaat
     3661 gcgaccaaac ctgccaaggc agcagcacct gatttgtcca ctgacatccc tagtgctaac
     3721 ccaacggcaa acagcaaagg taattgacct aaaactgcgt taccgccagc ttgtaaaaaa
     3781 tgagcgacaa tgtcgttact ataagatgcc caccagtttc cgatccctac tagtaaagca
     3841 gcagcaggta agaccgcaac tggaagttga agggaacgtc ccattcgttg tagatatgct
     3901 ttcaattttt ttcctcctca tgattgttaa catttgtttg tacgattgcg cttcttaaac
     3961 gctcgatatg gatggccaag taacccagtt cttctttcgt gatcgcgagc tggtattctt
     4021 tggtcagcaa cacacggatt tttttagaaa tcttatagct ttctgggtat tttcgtttga
     4081 ccatttctac gatttccgtg tctaaaacag catattgttg cgcatgggtt cgttgtagaa
     4141 gtagtcgtaa atgattcacc agtcttgaat aattcagtga aaatggttct gaatggatat
     4201 ctgtttccaa ttctgattcc actaaatgga tgatgtcaga tacaatcgtc acttcacgga
     4261 tactgcgatg gttattgttc cgaccgcttc gtccgctgtg aatatggatt gcgatatatc
     4321 ctgcttcgtc ataagtatat ggaatcccaa gctcttttgt cagatactct actgcccatt
     4381 gagcaatact gaattcatca ctgtaaagca cttcgatttc tcttaaaagt ttgttgcgga
     4441 tgacgatacc gttattaatg ttctccgcag aaaaagcaat gtggtcggtc aaagcgatca
     4501 atacatgttc attcaacttt tccatcaaaa cagtttccgc atgatcaatg atcgtttctg
     4561 ctgcaaataa aaagcgttca tcaatctgat taagtaaatt ttgcaacttg atttgacctt
     4621 ctggttccat gacaaatatc cgttcgacat cacgatcaaa aatcaaatca tttctttttc
     4681 tgtcaaatcc aatccctttt ccaatagcta ctttttcctg tccaccgtca tctaccaata
     4741 ctgcattttg attaaagatc tttttgattt tcatgcgcgc accctttttg aagatttggt
     4801 tttcacgttt gctagaccag ttgattgcgg ccaaacaaaa atagacacga aaaacctcct
     4861 agggaaacca tgttcctcag agatttttca tgcctagtat gatctagtca catgaaacga
     4921 attattgtta acgtaatcaa ttttatatag acagttatgt tttgtcaatt ctttttttat
     4981 aattatagta atttactgga attatcagaa ataaaaggaa gaaataaatt aagaaactta
     5041 tgttatttaa tcgttggatc ttttgcttat cttattcgtt gttaaaaagc tgaaagcgaa
     5101 ggaaaaaaac tctacttaac tgtatttctc cgttaggaca tctaaaaatt tacgagcagc
     5161 aggagaaaga ggctggtctt ttttccaaat gagattcagt gaagcttgta atggcggatc
     5221 gagtgggatg aatatttttt ccgtcccata agtattgatg atcccatcta gagctaatgc
     5281 accaaaagtc ccgttatcga ccatcaaaga agcattgtac aataagttat atgttccgac
     5341 gatcgataac tgatctatgc tgaaacctag ccattcagct agttgactgt ccactgacgt
     5401 ttgccgggaa atgatcaaag gaacagtttt cagttcctgc ggatggatga cggtttgctt
     5461 tgctaaagga tgatttcgat gtatcaaaag tccccaacga tcttgggctg gcagtcgaaa
     5521 tgactggtat ttttgtttgt ttgtcggttc gattacgagt ccaaaatcaa gtaatccgct
     5581 atccagtttg tctatgatat catctgcatt cccgctatat aaatgaaact ggatatcagg
     5641 atgcgccttg atcaacgaat gaaggctatc agaaatgaaa gagattgctt tagtttcgcc
     5701 tccgccaatg tagatttctc cagagatgat tgcgtcattt gttaaattgt tcgccgtttt
     5761 ttctaccaac gacaagattt cttttccttt tgaataaaga tatcgaccag aatccgtcaa
     5821 ctgtatctct cgggagcccc gagtgaaaag tgtcacacca agttcttctt ctaaatcttt
     5881 cagttgtttt gagagagtag gttgcgtgat atgcagagat tttgctgctc ggctgatatt
     5941 tttttctcta gctactgcta aaaaatacgt cagtatacga atatccattt tttccctcct
     6001 gtttttaaaa aagcatacca ctaagtatag ttaaaaggaa tagttaagta tgttttataa
     6061 gtattagaca tgataagccg tccgttataa agtaaggata aaaggaggga gcaaaatgac
     6121 aagcctcatc gaaaaacaaa tcgtcaataa agagattcga gtaacggatc cgctctttga
     6181 agtgatccat cagatccaag ctgaaaacga agagccttta gcagcgctga atactgggta
     6241 ccatgaacca gcagatatcc gcaaacggct agaaaatatc attagtgaca aagtagatga
     6301 tacggtgact gttctgctgc ctttttatac agatttcggc aaacatatta gtatagggaa
     6361 aaatgtcttt atcaatcgtc aagtgatgtt cgttgactta ggtggcatct gtttagaaga
     6421 ttcagtattg attggtcctc gtgtcaacct catcacggtg aaccatctaa ccgatccaaa
     6481 agaacgaaga ggcttgtcag taaagccaat ccacatcaag aaaaatgctt ggatcggagc
     6541 aggagcaact attctgccag gcgtaacgat tggcgagaat gccattgttg cagcgaatgc
     6601 aactgttacg aaagatgtcc cagacaatac catcgttgca ggaatccctg ccaaaatcgt
     6661 caaacctgtc gaacggatta tttgaacagt aagatgaaat aagagatagt gagtataagc
     6721 aagagatttt tcaaaacagg aggagaatag aatgaatgat ttatcagaaa aagtgattgt
     6781 catcatgggc gcttcaagcg gaatcggtga agcaacaacg aagctattag cagaaaaagg
     6841 ggctaaacta gttatagctg cacgccgaga agatcgccta aaagcaataa aagaatcttt
     6901 accagaagct gagctatata ttcaaacagc agatgtaaga gattttgcac aagtccaagc
     6961 agttatcgat ttagcgatgg aaaagttcgg tcgaatcgat gtattgtaca ataatgcagg
     7021 aatcatgcca accgctccat tagttgaagg tcaccgtgat gaatggcaaa atatgcttga
     7081 tatcaacatc atgggtgtgt taaatggaat ttccgccgta cttccaatca tggaaaaaca
     7141 aaaatctggg catatcattt caactgattc tgtggcggga catgttgttt atcctgattc
     7201 cgccgtttat tgcggaacaa aatttgctgt ccgtgcaatc atggaaggat tacgtcaaga
     7261 gcaacggcaa aataacatca aatctacgat catctctcca ggagctgttc aaactgaatt
     7321 atatcaaacc atcagcgaca aaaaggctgc tcttgaactt catgaagcgc aaaaagaatg
     7381 gggattgacc tccgaagata tagcttctgc tgtagcattt gcgatagaaa cgccagaccg
     7441 gatgtctgtc agtaacatga ttatccgtcc gacgacacag gaagtataat ccataacgga
     7501 taatatccaa agagaattac ttgttaaata gttattcaaa cgaccatcaa tttaaggcgt
     7561 gaatggagaa gaatattctt catttacgtc tttttatgta gaaaaagcaa attcatcaaa
     7621 aagaaacaaa tgttatttaa atatggacat tgacttgctt ttactttttt agttacactg
     7681 ttatttagga aaagtttcat aattcaaagg agaaaaaaga tgaaagcaaa gaatttatta
     7741 tttagcgtta ttcttttagg aaatctagca gttgccaaca caacagtgct agctgaagaa
     7801 tcgacagaaa ctgtcgcaac tcagacatcg agttcttcag aaacaggaat tgaaaacgat
     7861 acagagagta ctttgcagtc agctactgtc tcagaaaata caatttcaac cgaagaacca
     7921 acaactgaaa caacagaaca aacacaaacc gaagacagta catcagcaga agctcagaac
     7981 aatccagaga atgcagaaga aatcacgaac caattgacag atgggcaaac attgacacat
     8041 acccatcaag cagatttgtc tgaattcgaa aactatactg gctatgcttt ttatgctcgc
     8101 tcatctgcta attctcaaca agcgttcatt gatagtattg cctcaactgc acaaagtttg
     8161 gcaagtgcca atgacttata tgcatcagtc atgattgctc aagccatcgt tgaaagtggc
     8221 tgggggaaca gtactttggc ttctgcccct aattacaatc tatttgggat taaaggaagc
     8281 tataatggcc aatctgttac gatgcctact tcggaatatg tgaatgggca atggattacg
     8341 gtgaatgccg cttttagaaa atatccttcg tacaaagaat cattgcaaga taacgtaacg
     8401 gtattgaaga cgacttcttt tcagccaggc gtttactact attccggtgc atggaaaagc
     8461 aatacgaact cttacaaaga tgctacggct tggctaacag gtcgttatgc cactgcgcct
     8521 aattatggtt ctacattgaa caatgtaatc gaaacctata atctgacaca gtatgataca
     8581 gcaccaactt caacttctcc gatgtatcgt ctgtataacc gtcatacagg agaacattta
     8641 tatacattga atgccggaga aaaaaattat ctgccaactg taggctggga atatgaaggg
     8701 attgcttggc aagcaccaaa cagcggacaa cctgtctacc gtttatacaa tccttacagt
     8761 ggagaccatc attacacaat ggcacaatct gagatcaact ctttaacaaa aatcggttgg
     8821 cgttatgaag gtctaagttt ttattctggt ggttccaagc caatttatcg actatttaat
     8881 ccaaatgaaa aaactggtac ccatcactat accttgagtg ctaaggaaag agattggcta
     8941 acaccgatgg gctggagata tgaaggaata ggattttatg gttattaaaa agaggtagtg
     9001 aaaaagctgc cctgcatcaa gtagtaagaa cagccaaaca aaaacagatt ttcatgtttt
     9061 ttgtttggtt gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag
     9121 gttgtgacaa catcttgtca taactttttt attactgttt aagcatactg atccaatttc
     9181 taatattcga cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaattt
     9241 tctactgcag aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg
     9301 ttgtgctttc tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt
     9361 ttctattgag agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat
     9421 tctattattg acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct
     9481 ttagaagaga ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag
     9541 atctttgaag aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa
     9601 gaagaaaatc cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca
     9661 gttactcgtc atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct
     9721 cagaacaacc gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcgagcat
     9781 tgtgaaatgg accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa
     9841 atgaaacaat ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca
     9901 gaaacgaaag aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg
     9961 tagaagatcc gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga
    10021 aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt
    10081 ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag
    10141 acaaaatgat acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata
    10201 tggcgaagaa atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga
    10261 tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga
    10321 tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca
    10381 tggctatgaa acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg
    10441 tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga
    10501 aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt
    10561 cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag
    10621 gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa
    10681 ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg
    10741 aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg
    10801 ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt
    10861 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
    10921 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
    10981 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
    11041 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
    11101 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
    11161 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
    11221 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
    11281 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
    11341 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
    11401 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
    11461 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
    11521 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
    11581 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc
    11641 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
    11701 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
    11761 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
    11821 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
    11881 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
    11941 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
    12001 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
    12061 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
    12121 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
    12181 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
    12241 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
    12301 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
    12361 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
    12421 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
    12481 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
    12541 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
    12601 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
    12661 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
    12721 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
    12781 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
    12841 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
    12901 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
    12961 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
    13021 tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact
    13081 caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa
    13141 gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga
    13201 ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga
    13261 atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa
    13321 attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac
    13381 gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg
    13441 tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt
    13501 cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca
    13561 cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt
    13621 tattatatca cagtaaagtt tccacgtttt acatgtgcat atttacatgt aaaataaatc
    13681 atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa
    13741 gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt
    13801 atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag
    13861 ttaaacgcta cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt
    13921 gtggtgatgc tatttacagg tttcttatct agtcggatcg gtttgaaaaa aacattgatg
    13981 acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga
    14041 atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt ctctactgct
    14101 ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat gatgtatgaa
    14161 gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt gggcagtggt
    14221 tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt tatagcccta
    14281 gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg ggaaggtgtc
    14341 aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt atataatttc
    14401 ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc tgaattagaa
    14461 gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat tttcgttgca
    14521 ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt tgtcttattc
    14581 attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt accgatcagc
    14641 attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac gattgctatg
    14701 gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt ccgctttttc
    14761 ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg tgaaagctat
    14821 gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat ttatcaccgt
    14881 aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa agctgtcctg
    14941 catcaagtca taagaa
//
LOCUS       DAFELO010000204         8061 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.093, whole genome shotgun sequence.
ACCESSION   DAFELO010000204
VERSION     DAFELO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.093"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            128..1564
                     /locus_tag="LP452_002983"
     CDS             128..1564
                     /locus_tag="LP452_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2015626.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LP452_002984"
     CDS             1753..4200
                     /locus_tag="LP452_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL2015627.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LP452_002985"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LP452_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL2015628.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LP452_002986"
     CDS             complement(5690..6793)
                     /locus_tag="LP452_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2015629.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LP452_002987"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LP452_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL2015630.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2695 a         1375 c         1751 g         2240 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAFELO010000205         7769 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.095, whole genome shotgun sequence.
ACCESSION   DAFELO010000205
VERSION     DAFELO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.095"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            234..1202
                     /locus_tag="LP452_002988"
     CDS             234..1202
                     /locus_tag="LP452_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL2015631.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1195..2226
                     /gene="vanA"
                     /locus_tag="LP452_002989"
     CDS             1195..2226
                     /gene="vanA"
                     /locus_tag="LP452_002989"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL2015632.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2232..2840
                     /locus_tag="LP452_002990"
     CDS             2232..2840
                     /locus_tag="LP452_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL2015633.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3268..4179
                     /locus_tag="LP452_002991"
     CDS             3268..4179
                     /locus_tag="LP452_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL2015634.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4332..4817
                     /gene="vanZ"
                     /locus_tag="LP452_002992"
     CDS             4332..4817
                     /gene="vanZ"
                     /locus_tag="LP452_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL2015635.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5104..5433)
                     /locus_tag="LP452_002993"
     CDS             complement(5104..5433)
                     /locus_tag="LP452_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2015636.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5738..6067
                     /locus_tag="LP452_002994"
     CDS             5738..6067
                     /locus_tag="LP452_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2015637.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6057..6344
                     /locus_tag="LP452_002995"
     CDS             6057..6344
                     /locus_tag="LP452_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2015638.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6623..7186)
                     /locus_tag="LP452_002996"
     CDS             complement(6623..7186)
                     /locus_tag="LP452_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2015639.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2564 a         1294 c         1545 g         2366 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctggtag
      121 acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc
      181 attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata
      241 acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt
      301 cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca
      361 aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta
      421 ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca
      481 atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact
      541 cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa
      601 aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg
      661 tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg
      721 agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg
      781 taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc
      841 cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag
      901 gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag
      961 cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt
     1021 ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa
     1081 gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg
     1141 ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat
     1201 agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa
     1261 tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga
     1321 attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac
     1381 aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag
     1441 aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca
     1501 ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc
     1561 gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat
     1621 gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct
     1681 acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa
     1741 aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc
     1801 aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac
     1861 agtgccgcgt tagctgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt
     1921 attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca
     1981 gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg
     2041 ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta
     2101 ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg
     2161 gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag
     2221 gggtgataag catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt
     2281 gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag
     2341 taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg
     2401 ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaat cgtgctgtaa
     2461 actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc
     2521 ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata
     2581 gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa
     2641 tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat
     2701 gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt gggtttgaag
     2761 catatagcct cgaatggtgg cactatgtat taagagacga accatacccc aatagctatt
     2821 ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat aagctaactc
     2881 tttcggcagg aaacccgacg tatgtaactg gttcttaggg aatttatata tagtagatag
     2941 tattgaagat gtaaggcaga gcgatattgc ggtcattatc tgcgtgcgct gcggcaagat
     3001 agcctgataa taagactgat cgcatagagg ggtggtattt cacaccgccc attgtcaaca
     3061 ggcagttcag cctcgttaaa ttcagcatgg gtatcactta tgaaaattca tctacattgg
     3121 tgataatagt aaatccagta gggcgaaata attgactgta atttacgggg caaaacggca
     3181 caatctcaaa cgagattgtg ccgtttaagg ggaagattct agaaatattt catacttcca
     3241 actatatagt taaggaggag actgaaaatg aagaagttgt tttttttatt gttattgtta
     3301 ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca ggaaaaagtc
     3361 gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg gacttctgaa
     3421 aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct gctattaatc
     3481 aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa tttatctaaa
     3541 catgacgaat taataaatgg atacgggttg cttgatagta atatttatat gtcaaaagaa
     3601 atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt tagtcatttt
     3661 attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca agaaatgggg
     3721 gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc actagatgta
     3781 ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga agaaaatgct
     3841 tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac aggaattcaa
     3901 tatgaaccat ggcatattcg ctatgttggt ttaccacata gtgcgattat gaaagaaaag
     3961 aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat ttctgttagt
     4021 gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac caccattcat
     4081 gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt aattgtgaca
     4141 gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa tgaaaccaac
     4201 gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc tttttaattt
     4261 aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca aaagaattag
     4321 aggaaattat attgggaaaa atattatcta gaggattgct agctttatat ttagtgacac
     4381 taatctggtt agtgttattc aaattacaat acaatatttt atcagtattt aattatcatc
     4441 aaagaagtct taacttgact ccatttactg ctactgggaa tttcagagag atgatagata
     4501 atgttataat ctttattcca tttggcttgc ttttgaatgt caattttaaa gaaatcggat
     4561 ttttacctaa gtttgctttt gtactggttt taagtcttac ttttgaaata attcaattta
     4621 tcttcgctat tggagcgaca gacataacag atgtaattac aaatactgtt ggaggctttc
     4681 ttggactgaa attatatggt ttaagcaata agcatatgaa tcaaaaaaaa ttagacagag
     4741 ttattatttt tgtaggtata cttttgctcg tattattgct cgtttaccgt acccatttaa
     4801 gaataaatta cgtgtaagat gtctaaatca agcaatctga tctttcatac acataaagat
     4861 attgaatgaa ttggattaga tggaaaacgg gatgtgggga aactcgcccg taggtgtgaa
     4921 gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa cactatagta acaaagaaag
     4981 cccaattatc aattttagtg ctgaggaatt ggtctcttta ataaatttcc ttaacgttgt
     5041 aaatccgcat tttcctgacg gtacccccaa actaagattt catagataga ttttctgata
     5101 actttaaaaa acacgaatag cggaaaggat gtcttcattc gctaaagagt aataaactac
     5161 ttttccatct tgacgtgatt ttgccattcc atgttttttt aaaaaacgta aatggtgtga
     5221 cgttgtagcg acactagcag taagtatttc tgctaaatca cagacgcaca tttccttata
     5281 ggtttctaaa gcatagaaaa ttttaattct agaaaaatcg ctgaaacatt tccctaacac
     5341 aagtaagttt gaaaaatctt tttgatccaa tttatttttt attccattta ctttttcttt
     5401 atgtacgaca gtcacttggc aaactttttt cattcatctc acctcacatt caaacgatta
     5461 tttgaatgaa gtatacctat ttgatcactc ttttgtcaat ctaccttaat tccaatacac
     5521 tattataagc aatttcatgt attgaagtaa acttacttgc aagtgcgttt ttgattccaa
     5581 gttaaaaaca tattattatt ctacataatt tcctaatcaa ataaaactcc tttgaaaatt
     5641 gttctaaagg agttttatta ttttgtattt ctactaaaaa tagcgtacaa tgtaattact
     5701 aagtaattac gaaatgtaaa ataatggagg aaataatatg attacaaaaa caagaaaaca
     5761 aggaaattct attatgttaa cggtccctaa agaattcgat gtgcccaatg gtgttgaagt
     5821 agaagctaag cttgtagaga atggtatcct ttatgagttt gtagagccta aaaaagaatt
     5881 ttttgatttt agtgaagata ttttggctga tattctttca gaaggttata gcaaacaaga
     5941 gatcttaaaa gaatttagaa atagaaaaaa tgcgttaact tctgcattta aatctattgc
     6001 tgaagatact gttgcaaatt ctaaaccgat gacaaaagag gagttggcag cagaaattgg
     6061 cttatgagat tctaccttct aaacacgtaa taaagtatct aaaaaaatta aaagagaaaa
     6121 cattaaaaga acaattttta acgataattt atgatgagat agcagttcgt cctcattcgg
     6181 gagaacaaaa aacgggtgat ttatcaggaa tttgggctat tggattcaag tatgcaggta
     6241 cgacatatcg cgttgcatat gaaattaaag ataatgcagt gatacctatc ttactatgtg
     6301 gaacgcatga aaatttttat gaacaattaa aaaagattag gtaagaaccc aatgtttcaa
     6361 cttgagagga tagacgacac tttaaaagat aatttttaac agctgagagt tgtaataccc
     6421 tataatttta aaccactaac ctatatattt atgaaaaata gaaaaaatta aaacgcattt
     6481 taagcctctt tcaaattaaa agcttcatcc ttatgcacaa agagggctta aaatgcgtta
     6541 ttttatattg ttttttagta ataataaaaa aatcactcta attctcccat aaatattata
     6601 gattcctaaa gattaaaata gattattccc tattgtcttt taagtagcgg tagagtgttg
     6661 ccttgctaat accagtcatt tccgtaattt cttttacgct ataaacctga cttttataca
     6721 attttactgc tcgatcaaca tctttctggt tcactcgtgg acggccacct ttacgacctc
     6781 tcgcacgtgc acttttcaaa ccttctttgg tacgttcaac aattaaatca cgttcgaact
     6841 gactaaatgc ttggaagaca gtcatcatta agcgtccgtg aggcgtgctg gtatcgaagt
     6901 tttctttcaa gctcaccagc ttaacatggt gttcttcaaa ataggtaaca aggtcaatca
     6961 aatcctttgt actacgaccc aatcgagaaa aactttctac aattaccgta tctcccggac
     7021 gaattttatc ttttaaacga tttagttggg gacgaaccgc tttggttccc gtcattttct
     7081 cagttaggat ttcattacaa ttttgttcat tgagtaagtc taattgacga tttagttctt
     7141 gtgaacgtgt actcactcgg gcataaccat aaatgtatcc actcatgctt ttcttccttt
     7201 cgtactttaa ttctggcttg attgtatcat aaaagggagt tatagataca tagttaatgc
     7261 aacaagttat gatacaaaat atgacctctt ttatttaata aaagaggtca tttcattttg
     7321 tctcataatc gattgttttt gatacgagtg tgtatacgaa ataaacaaaa aaatcttaag
     7381 taaatttcta gatacctaat ttaatcctaa ctagtgtatc ctaaagttaa taacgagttt
     7441 atcacaatga aaattataaa atgctcccat attagatata acatgcaata tttgaattag
     7501 aactacaaaa tttattcgtt ttataaagta aataatgttg agatactaag gaatagaata
     7561 ttataaggaa ttgagctgaa tgtattggat aaaaaaagaa taactttatt agagatggac
     7621 tataaaaatg ctcgtaattt tcttctgaag tctgaaagct atttgaatgc tcagctacca
     7681 gaatatatta gttttgacga aactttaaaa tctgctaaaa aagttttaac ttcgccgttt
     7741 gtataattaa gcgagacaaa taaaaaagc
//
LOCUS       DAFELO010000206         5290 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.118, whole genome shotgun sequence.
ACCESSION   DAFELO010000206
VERSION     DAFELO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.118"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            187..852
                     /locus_tag="LP452_002997"
                     /pseudo
     CDS             187..852
                     /locus_tag="LP452_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            888..1133
                     /locus_tag="LP452_002998"
     CDS             888..1133
                     /locus_tag="LP452_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2015640.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1236..2105
                     /locus_tag="LP452_002999"
     CDS             1236..2105
                     /locus_tag="LP452_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL2015641.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2086..2820
                     /locus_tag="LP452_003000"
     CDS             2086..2820
                     /locus_tag="LP452_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2015642.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2853..3768
                     /gene="ant(6)"
                     /locus_tag="LP452_003001"
                     /pseudo
     CDS             2853..3768
                     /gene="ant(6)"
                     /locus_tag="LP452_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            3765..4307
                     /gene="sat4"
                     /locus_tag="LP452_003002"
     CDS             3765..4307
                     /gene="sat4"
                     /locus_tag="LP452_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL2015643.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4400..5194
                     /locus_tag="LP452_003003"
                     /note="aph(3')-IIIa"
     CDS             4400..5194
                     /locus_tag="LP452_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL2015644.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         1810 a          785 c         1160 g         1535 t
ORIGIN      
        1 gattttatct ctttttcaaa ataaacatgg ggctgaccca aaagtagctc ctatatgaaa
       61 aatagaggtc tttccattta gatagtagtg gaaagacctc tatttatctt ttgaaagtga
      121 atacaaaaat aaagcacact ctgctataat ataggtgatt aatccatttg agagaggtgc
      181 tttatcatgt atcaaaatta taccataaac caactttgtt tgcctataga tcttgaaatt
      241 aaattagaag aaaatgattt tgctcatgcc attgtgcagt ttgttgactc tattcccgat
      301 gaagtctttc ttccttatta tcaatctatg ggtagacccc aatatcatcc tcgtatgcta
      361 ctttccatta ttctttgtgc ctatattcaa ggggtttatt cgggccgcca aatacaaaat
      421 atgctgatag atagtattcg gatgcgctac ttatctcaag agcaattccc taactttcgt
      481 actattaatc gttttcgcgt tcatccgatt atgaacgata ttctagatca ttcatttgtt
      541 caatttcgtg agctactggt tcaaagtggc ttaatcagtg gcagtgctct atacattgat
      601 ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg tttggaaaaa aagtatttta
      661 aagtataagg atagccttga tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca
      721 aaaatcttac ctgagttaat agatgattta aaggatgaac tttctataga agagattgaa
      781 aggattcgtc aatctttgga agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc
      841 gaaacagtag aagggggctg acccattatt aataaggagt ggacaacatg attgatgtaa
      901 atgagctatt ggaagaagct attagagaaa ctgaaaatct aaatgatggc gaagtttttc
      961 ttgttaaaga tttattcaag ggatatgtgg ggaataggat acccagaaag gatcgacttc
     1021 tgcttggaac gttattttta aaccatgtta gcaaaatgaa tggtaattta aaggctattg
     1081 aaaagacttc atctaatcag cagaggtata aaaagacaat tgataagttt taactatctt
     1141 ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc aaaagatttg gcttggaatt
     1201 tttacagcag tgaatcgata aaaggagcat gcttgatggt agataatatt attaaatcag
     1261 tagcagagaa attatcctct ctgtcttata tagaaggtat tgttttaggt ggttcacgtg
     1321 caaggggcac ccatacagag gattcggata tagatatcgg catctattac aattcagaat
     1381 catttgacat aaatactatt aatcaattcg ctacaaagct ggatgatgag catagaaata
     1441 accttgttgt acctcccgga gcatggggtg attggattaa tggcggcgga tggttagtca
     1501 taaacgggta tcatgtggat ttaattttac gtgatattaa acgtgtggaa caaataatga
     1561 aagatacaga gcacggaatt gttactgcca attatcagac tgggcatccc catggttata
     1621 ttagtgcaat gtatcgagga gaattagcga ttagcaaaat actatatgct aagaatgaaa
     1681 gcttatgcga attaaaaaaa caggcagaaa cttatcccaa tgctttgcag aaaagtttag
     1741 ttaacttttt tatgtttgaa gcagggttct ctttaatgtt tgtaaaagca aattcgggaa
     1801 cagacgataa atattatatt gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag
     1861 tgttatttgc atgtaataat gcttattgta tcaacgaaaa gaaagctata aaactgcttg
     1921 aaacttttga acataaacct gaaaaatata ccgagaaggt aaatcatatt tttgaagtac
     1981 tcggtatctc actttttgaa tgctacgaca tgaccgagaa gctttataat gaagtgaatg
     2041 aaattgtatc ggagataaat aactttttaa acgaggagag ttcagatgaa agaaaacaaa
     2101 tatgatgata atatattttt tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg
     2161 gctggtgcgg gagaatggga gactttgaaa aagatgctac ctgattttaa gggtaagcgt
     2221 gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct
     2281 tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc
     2341 cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag
     2401 agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta
     2461 ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat
     2521 cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg
     2581 catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa
     2641 aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt
     2701 tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg
     2761 atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa
     2821 taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt
     2881 tagtactttc tttagcagaa caggatgaac gtattcgaat tgtgaccctt gaggggtcac
     2941 gcgcaaatat taatatacct aaagatgaat ttcaggatta tgatattaca tattttgtaa
     3001 gtgatataga acctagaacc gtttatatct aatgatgact ggcttaatca atttgggaat
     3061 ataataatga tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt
     3121 tcctatctta tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa
     3181 gagttagata attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt
     3241 agaattaaaa gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca
     3301 agggagtatg atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga
     3361 ttgtgccgta aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag
     3421 ctgctgagaa tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc
     3481 aagaactata agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc
     3541 acctaccgga tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg
     3601 ttcagggcgg tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat
     3661 aggaatataa caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc
     3721 ctggatagca catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga
     3781 aagcagggca cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga
     3841 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     3901 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     3961 atattgacaa tactgataag ataatatatc tttactacca agacgataaa tgcgtcggaa
     4021 aagttaaact gcgaaaaaat tggaaccggt acgcttatat agaagatatc gccgtatgta
     4081 aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa
     4141 agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta
     4201 aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
     4261 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
     4321 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
     4381 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
     4441 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     4501 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     4561 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     4621 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     4681 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     4741 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     4801 tagcttagac agccgattag ccgaattgga ttacttactg aataacgatc tggccgatgt
     4861 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     4921 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     4981 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     5041 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     5101 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     5161 aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg tctaaaaagc
     5221 tttaactaca agctttttag acatctaatc ttttctggct ctttgtcaaa tgatgttggt
     5281 aaagagaaat
//
LOCUS       DAFELO010000207         4544 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.125, whole genome shotgun sequence.
ACCESSION   DAFELO010000207
VERSION     DAFELO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.125"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            348..1562
                     /locus_tag="LP452_003004"
     CDS             348..1562
                     /locus_tag="LP452_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL2015645.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1593..1811
                     /locus_tag="LP452_003005"
                     /pseudo
     CDS             1593..1811
                     /locus_tag="LP452_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2230..2703
                     /gene="dfrF"
                     /locus_tag="LP452_003006"
     CDS             2230..2703
                     /gene="dfrF"
                     /locus_tag="LP452_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL2015646.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2830..3486
                     /locus_tag="LP452_003007"
     CDS             2830..3486
                     /locus_tag="LP452_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL2015647.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3668..4198
                     /locus_tag="LP452_003008"
     CDS             3668..4198
                     /locus_tag="LP452_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2015648.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1520 a          667 c          960 g         1397 t
ORIGIN      
        1 atagaatgat tttctatcag tcctagaaat tatagagccg aacaaatttg agaaccgtag
       61 cgacactcgc tgcggttctt ttttatatat tttatatgaa gtattcttta ttccttatga
      121 aaaaatatct ttaaaaataa aaaaagacga aaataaacgg ttttctataa aaagaagacc
      181 gtttattttt tgtctttatt tgtgtgaaaa acgaaaaaga gcacaacaca tcaagctttt
      241 attaattgtc tgtcttttat aaatgcaagt attaattcaa aaaaacagaa gaaaaaaaca
      301 actacacctt cttttatact cgtaatcgta gaaaggaggc gaaaaaaatg aatacattac
      361 ctatcaatac aggagataca gcattcatga ttttatgtac agcaatggtt tgcctgatga
      421 ctcctggtct agcttttttt tacggcggat tagcacggaa gaataatata ctaacgatca
      481 tgtctcaatc ttttctctcc gttgggataa caacgattat gtgggtattt ggtggattcg
      541 gattggcctt tggacgggat atcggcggtg ttattggaaa cccggcagat ttctttttga
      601 tgagacatgt tacaagcttc cccaacatgt ttcatggtgc aacgattcca tttttaatgt
      661 tctttcttta ccaactgatg tttatcgtga ttaccgttcc tttgatgaca ggagcttttg
      721 cagggagatt gaatttaaaa ggctatatta tacttgttat tttttggaat ctgctgattt
      781 atttccctgt ttgccactgg atatggggtg gcggattttt ggatcagatg ggatttagag
      841 atttcgctgg tggagcagtc atccacacga cagctggatt cggctcttta gcatgtatcc
      901 tcacacttgg tcaaagaaag attcctttag gagaaagaag tcagcatagc agtcttatgg
      961 cagctgctat cggtacagga ctgctttggt ttggctggtt cggttttaat tccggtggtg
     1021 cacttcgagc agatgaacaa gcagtcaatg catttggttc aacattcatc gctcttgcat
     1081 ttgcgatgat tacttggctg atcattgcaa aagtaaaagg aaatggcttt gattttgtcg
     1141 acgtactaac aggatccgtt gctggattgg cagccattac gccttgtgcc ggttatgtag
     1201 aagcaaaaag tgccatgctg attgggatca ttgcgggtat cgtctgtcat gctgctgttg
     1261 attttcgtaa aaagaaacaa tgggatgatg cacttgatgt gtggggtgtt catggcatgg
     1321 gcggattcac aggtacgatt ctgataggga tctttgcatc taacagccat ttactaacga
     1381 atccaagcag ctggtacttc ttagggattc aagtgattgg tgtcatcatt actgccgtgt
     1441 atgcctatgt gctgacaaca gtgattttaa aatcggcgat gcactttaca acgatcacta
     1501 ctacaaaaga agaacaagaa gaaggattgg actttttctt ctttggcaac aaactcaatt
     1561 gaaacaaatt caaatagaaa agaggaagat tcatgcaaca aagcaaaacg aaaattcctg
     1621 caaaaaaatt aacgttcttt ggctttttgg ctatgaccat ctcaatggtc gtttcattat
     1681 atgaatatcc cacattcgcg acctcaggct tcagcttagt atttttcttg ttattaggcg
     1741 gtttgctatg gtttatccct gtggcattgt gcgcagcaga gatggcaacg gtgaaaggat
     1801 gggaaaaagg tgtttcccaa acaaaaagat attaagaaac agcccatgtt tacctgcttg
     1861 caaacaaaag aatattgcta ctagactaag agatactaca taagtgtgtt ggtatctctt
     1921 ttttgttgcc gtgaaacagc agactatttc atttctgctt gattacctta aaacttatta
     1981 gtctatggtt tgtcaagggc ttgttgcgtg agaaatgctg aatatcaccc tttacaaaac
     2041 ataggctgat aagtaacttt tcaaaagcag aaaagaaatg gcaatgctta taacaatatg
     2101 atataatgca gatgaagaca aaaaaggaat ttgtcgagct ggttagttcg agataatttt
     2161 gagtttgtta agatgtggtg cacttgatgg aatcacaggt acgacatcat attatggagg
     2221 atgaaataga tgataggttt gattgttgcg aggtcaaaga ataatgttat aggcaagaat
     2281 ggtaatatac catggaaaat aaagggagaa caaaagcaat ttagagagtt aacaacgggt
     2341 aatgtggtta ttatggggcg aaagtcttat gaagaaatcg gtcatccgtt gcctaataga
     2401 atgaatattg ttgtttccac cacaacagag tatcaaggag ataatttagt ttcagttaaa
     2461 tcattagaag atgcattatt attggctaaa ggacgagatg tatacatatc tggtggatat
     2521 ggactattta aggaagcttt gcaaatagta gataaaatgt atatcacaga agtagattta
     2581 aatattgaag atggagatac attctttcca gaatttgata tcaatgattt tgaagttttg
     2641 ataggggaaa cacttggtga ggaagtgaaa tatacgagaa cattttatgt aaggaaaaaa
     2701 tgaattgagt aaattttgga tttagggtgt tttcataaat atattgcttt ttatgcatat
     2761 ataaattgtt gtaagacaaa ttccagtttg tatggggcga taaaatcaga atttgagaga
     2821 ggattgaata tgaataagga atggtctgaa cttaataaaa caatgcaggc acaaataaaa
     2881 aagaaagata cttataagag gggtattgat actttactta ctttacgaag tcagttaata
     2941 caaaccttag tatctttcaa agaagaatta tgcagagaag attttaactc aatccccttc
     3001 ataaacgctg atggttatca tagtaagacg attgcttatt ctatttggca tattttccgc
     3061 attgaagata tagttgtgca tacagtgata aatgaagatg aacaagtatt ttttgcaggc
     3121 aattatcaag agcgtatcaa ttcacctatc attacaacag gaaatgaact catgaaacag
     3181 cagattgcag acttttcaaa acagcttaat ctggaagaat tatatttata tattttcgaa
     3241 gtatgggaaa gcactgagaa aatgcttgaa cggttatctt atgatgaatt gaaaagaaaa
     3301 ataccgaaag aaagaaaagg atacttagaa tcgttgaatg tagtaaacga caatgaaaaa
     3361 gcaatttggc tgattgatta ttggtgtaat aaagatattt gtggactaat tcaaatgccg
     3421 ttttcaagac attggattat gcacacagaa gcctgtttgc gtataaagaa caagatacat
     3481 tcataggcaa aaatttcagt ttgtagaact aaggaaattg gaatttaagg aggaataaag
     3541 tatggttagt gattttataa gagcagcatt tccatggatt ttaatgggtt tatttgtagc
     3601 aatcagttgt tcccttatga gtaaaaaaga gaaataaatt cctgtttgta tttataaaag
     3661 agaaaaaatg agaaaagtag ttttatttat tgccatgagc cttgacggat atattgcgga
     3721 cggtaatggt ggagtagctt ggctaaatgg tcatggaaat gacaatgaaa acatcgacac
     3781 ttatacagaa tttaccaaag atatagatac tgtcttaatg gggtggaata cttctcatca
     3841 ggttgttact gaactttctc cgcaagaatg ggtatataat aaatttacaa cttatgtatt
     3901 aacacataaa gagtgcaatt cttcccaaca aattcatttt acaagtgaaa atcctgtttt
     3961 attattggaa cggctaaaac aagaagcagg aaaggatatt tggatttgtg gcggagcaaa
     4021 tcttgttcag cagttggtaa gcaaaaatat gattgataaa tattacattt ctgttattcc
     4081 tactctgtta ggaaatgggg ttcgtctttt gggcaacata gacagggaaa taaagttaag
     4141 gctttgtaaa acacagactt ataacggaat aaccgatttg atttatatgc gtagataact
     4201 agagagaaac agaaaagctg ttaaaccgac ggagcgatcc gaccacgttg gcgggaaaat
     4261 ccttaagatt tttccgccat gcttgcaagg gggcggttcg cttcggcggt tcggcgagct
     4321 tgcgagccgt tcaaagcccc cgttatctaa caaggggggc acaaaaaaca agaaacacta
     4381 tacactaaca agcggaaaac gctgtattta taaggcaaaa ccccattttt acagtagtga
     4441 tataaattgt acctatatca ctatgaaaat tgaatagtaa atatgagaaa gacagttgat
     4501 ttcaaaaaat aggctctttg tcaaatgatg ttggtaaaga gaaa
//
LOCUS       DAFELO010000208         1650 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.163, whole genome shotgun sequence.
ACCESSION   DAFELO010000208
VERSION     DAFELO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.163"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            <1..1348
                     /locus_tag="LP452_003009"
     CDS             <1..1348
                     /locus_tag="LP452_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBL2015649.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..>1650)
                     /locus_tag="LP452_003010"
     CDS             complement(1478..>1650)
                     /locus_tag="LP452_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2015650.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          671 a          148 c          278 g          553 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta
//
LOCUS       DAFELO010000209         1081 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR44537_1
            SAMN20574206-rid13343073.guided.186, whole genome shotgun sequence.
ACCESSION   DAFELO010000209
VERSION     DAFELO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20574206
            Sequence Read Archive: SRR16917305
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177028.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177028.1
            Genome Coverage       :: 312x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:56:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,012
            CDSs (total)                      :: 2,948
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 64
            rRNAs                             :: 3, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 33 of 64
            Pseudo Genes (incomplete)         :: 27 of 64
            Pseudo Genes (internal stop)      :: 17 of 64
            Pseudo Genes (multiple problems)  :: 12 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20574206-rid13343073.guided.186"
                     /isolate="PR44537_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2020-01"
                     /collected_by="University of Michigan"
     gene            111..194
                     /locus_tag="LP452_003011"
     CDS             111..194
                     /locus_tag="LP452_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL2015651.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="LP452_003012"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="LP452_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL2015652.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          406 a          174 c          166 g          335 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 t
//
